CN115475120B - Composition for preparing plant extract with antiseptic and antibacterial effects, plant extract and application thereof - Google Patents
Composition for preparing plant extract with antiseptic and antibacterial effects, plant extract and application thereof Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Botany (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
The application relates to a composition for preparing a plant extract with antiseptic and antibacterial effects, a plant extract and application thereof. The composition for preparing the plant extract with the antiseptic and antibacterial effects comprises moutan bark, oregano, cinnamon, acanthopanax, peppermint, chicken peppers and garden balsam stems. The plant extract provided by the application has the effects of preventing corrosion, inhibiting bacteria and enhancing efficiency, and can reduce the dosage of the traditional preservative in the cosmetics, so that the cosmetics are safer, and the allergy rate is reduced.
Description
Technical Field
The application belongs to the technical field of cosmetics, and in particular relates to a composition for preparing a plant extract with an antiseptic and antibacterial effect, the plant extract and application thereof.
Background
The cosmetic is composed of various oil raw materials, water quality and functional components, provides various nutrients required by skin, and is a warm bed for microorganism breeding. In the use process of consumers, various microorganisms can not be brought into the bad storage environment due to the contact of the skin and the air for multiple times, so that the cosmetic spoilage is caused, and various skin health problems such as allergy, dermatitis and the like are caused. To ensure stable quality throughout the life cycle of the cosmetic, and to avoid microbial infections, preservatives are indispensable ingredients in the cosmetic formulation.
The chemical preservative can accumulate on the skin of a human body, and when a certain amount is accumulated, the aging of the skin can be accelerated; on the other hand, the preservative can also accelerate pigmentation and skin darkening. Frequent use of chemical preservatives may cause varying degrees of injury to the human body.
Along with environmental pollution and ultraviolet irradiation, the cosmetic products are frequently used, especially the use frequency of the skin care mask is increased, the skin allergy rate is greatly improved, and the market investigation data of 2018 show that 58% of global consumers consider that the skin is sensitive, so that the use of dangerous substances in the cosmetics is reduced, the addition amount of chemical preservatives is reduced, the traditional preservatives are replaced by plant extracts with the preservative effect, the skin allergy rate is reduced, and the cosmetic is a popular trend of current cosmetics.
The trend of no antiseptic addition is more and more popular in the market at present, the skin allergy rate is greatly reduced, the demand for plant extracts with antibacterial and antiseptic functions is higher and higher, and the antibacterial and antiseptic functions of single plant extracts are limited, so that the antiseptic for developing the plant composition becomes an important breakthrough direction for replacing chemical preservatives with plant preservatives.
Disclosure of Invention
Based on the problems existing in the prior art, the application aims to provide a plant extract which has the effects of resisting corrosion, inhibiting bacteria and enhancing efficiency, can reduce the dosage of traditional preservatives in cosmetics, ensures that the cosmetics are safer, and reduces the allergy rate.
It is an object of the present application to provide a composition for preparing a plant extract having antiseptic and bacteriostatic effects.
The second object of the present application is to provide a plant extract with antiseptic and antibacterial effects, which is prepared from the composition.
It is still another object of the present application to provide a method for preparing the above plant extract.
A fourth object of the present application is to provide the use of the above plant extracts in skin care products.
The fifth object of the present application is to provide a mask liquid, which comprises the plant extract.
The sixth object of the present application is to provide a method for preparing the mask liquid.
In a first aspect, the present application provides a composition for preparing a plant extract having antiseptic and bacteriostatic effects, comprising moutan bark, oregano, cinnamon, acanthopanax, peppermint, chicken pepper and garden balsam stem.
The cortex moutan, the origanum vulgare, the cinnamon, the acanthopanax, the peppermint, the chicken peppers and the speranskia herb all accord with the relevant regulations under each medicinal material item in Chinese pharmacopoeia (2015 edition) and national Chinese herbal medicine assembly.
Cortex moutan is dried root bark of Paeonia suffruticosa Paeonia suffruticosa Andr. Digging root in autumn, removing fine root and silt, peeling root bark, sun drying or scraping coarse bark, removing wood core, and sun drying. It is bitter and pungent in property and slightly cold in nature. It enters heart, liver and kidney meridians. Is mainly used for clearing heat and cooling blood, and activating blood circulation to dissipate blood stasis. Is used for treating heat entering nutrient blood, toxic heat, speckle, hematemesis, epistaxis, night fever, early cooling, no sweat, bone steaming, amenorrhea, dysmenorrhea, traumatic injury, carbuncle, swelling and sore.
Oregano, oregano Origani of Labiatae, oregano vulgare L. It is pungent and warm. The main indications are exterior syndrome relieving; regulating the flow of qi; clearing summer heat; induce diuresis. The main common cold is febrile; heatstroke; fullness of chest and diaphragm; abdominal pain, vomiting and diarrhea dysentery; jaundice; edema; taking down; children's cellulitis accumulation; measles; itching of the skin; sore pain; traumatic injury
Cinnamon is the dried bark of cinnamon Cinnamomum cassia Presl, a plant of the Lauraceae family. Peeling in autumn, and drying in the shade. It is pungent, sweet and hot. It enters kidney, spleen, heart and liver meridians. Is mainly used for tonifying fire and invigorating yang, inducing the fire to return to the origin, dispelling cold and relieving pain, warming and activating meridian, and is used for treating yang fistula, cold womb, cold pain of waist and knee, asthma due to kidney deficiency, deficient yang floating up, dizziness and conjunctival congestion, cold pain of heart and abdomen, deficient cold vomiting and diarrhea, cold hernia and abdominal pain, dysmenorrhea and amenorrhea.
Acanthopanax is the dried root and rhizome or stem of acanthopanax senticosus Acanthopanax senticosus (rupr. Et maxim.) Harms belonging to the family Araliaceae. Collected in spring and autumn, washed and dried. It is pungent and slightly bitter in nature and warm in nature. It enters spleen, kidney and heart meridians. Is mainly used for replenishing qi to invigorate the spleen, tonifying the kidney and soothing the nerves. Can be used for treating deficiency of spleen and lung qi, asthenia, inappetence, deficiency of both lung and kidney, cough, asthma, soreness of waist and knees due to kidney deficiency, deficiency of heart and spleen, insomnia, and dreaminess.
Peppermint is the dry aerial part of the Labiatae plant peppermint Mentha haplocalyx Briq. When the stem and leaf are luxuriant in summer and autumn or flowers bloom to three rounds, the branches are harvested and cut in a separated way on a sunny day, and dried in the sun or in the shade. It is pungent and cool. It enters lung and liver meridians. Mainly used for dispelling wind-heat, clearing head and eyes, relieving sore throat, promoting eruption, soothing liver and promoting qi circulation. Can be used for treating wind-heat type common cold, initial wind-warm syndrome, headache, conjunctival congestion, pharyngitis, aphtha, rubella, measles, chest distress and hypochondrium distention.
Fructus Zanthoxyli is dried mature fruit of Pers of Lauraceae plant fructus Zanthoxyli Litsea cube. Harvesting in autumn, drying, and removing impurities such as stems and leaves. It is pungent in nature and warm in nature, enters spleen, stomach, kidney and bladder meridians. Warm middle energizer, dispel cold, promote qi circulation and alleviate pain. Can be used for treating vomiting due to stomach cold, cold pain in the back, hernia, abdominal pain due to cold, stagnation of cold-dampness, and turbid urine.
Garden balsam stem, the plant Euphorbia species Euphorbia lathyris L Speranskia tuberculata (Bunge) Baill, the plant impatiens balsamina Impatiens balsamina L of the species Impatientiaceae, the plant Artemisia carob Incarvillea sinensis lam of the plant Artemisia of the family Bignoniaceae, the whole plant of Vicia amoena Fisch of the family Leguminosae, and the whole plant of Leguminosae, the plant Begonia yunnanensis Gaultheria yunnanensis (Franch.) Red. It is pungent and bitter in nature, warm in nature, enters liver and kidney meridians. Is mainly used for dispelling wind and eliminating dampness, and removing toxic materials and relieving pain. Can be used for treating rheumatic arthralgia; it is indicated for sores and ulcers and other sores with toxic swelling.
According to some embodiments of the present application, the composition comprises 1-5 parts by weight of moutan bark, 1-5 parts by weight of oregano, 1-5 parts by weight of cinnamon, 0.5-5 parts by weight of acanthopanax, 1-5 parts by weight of peppermint, 0.5-5 parts by weight of chicken pepper and 1-5 parts by weight of garden balsam stem.
According to a preferred embodiment of the present application, the composition comprises 2-5 parts by weight of moutan bark, 1-3 parts by weight of oregano, 1-3 parts by weight of cinnamon, 0.5-2 parts by weight of acanthopanax, 1-3 parts by weight of peppermint, 0.5-2 parts by weight of chicken pepper and 1-3 parts by weight of garden balsam stem.
In a second aspect, the present application provides a plant extract prepared from the composition of the first aspect.
In a third aspect, the present application provides a method for preparing a plant extract according to the second aspect, comprising the steps of:
s1: mixing the composition of the first aspect with a solvent, and extracting to obtain an extract;
s2: and carrying out solid-liquid separation on the extracting solution to obtain the plant extract.
According to some embodiments of the present application, in step S1, the solvent is a mixture of an organic solvent and water.
According to some embodiments of the present application, the mass ratio of the organic solvent to water is (1:2) - (2:1).
According to some embodiments of the present application, the mass ratio of the organic solvent to the water is 1:1.
according to some embodiments of the present application, the organic solvent comprises a C1-C5 alcohol compound. According to a preferred embodiment of the present application, the organic solvent is propylene glycol.
According to some embodiments of the present application, in step S1, the weight ratio of the composition to the solvent is 1 (10-20), and may be, for example, 1:10, 1:12, 1:14, 1:16, 1:17, 1:20, and any value therebetween.
According to some embodiments of the present application, in step S1, the mixing time is 0.5-3h, for example, may be 0.5h, 0.8h, 1h, 1.2h, 1.5h, 1.8h, 2h, 2.2h, 2.5h, 2.8h, 3h, and any value therebetween. According to a preferred embodiment of the present application, in step S1, the mixing time is 1-2h.
According to some embodiments of the present application, in step S1, the temperature of the extraction is 60-90 ℃, for example, 60 ℃, 65 ℃, 70 ℃, 75 ℃, 80 ℃, 85 ℃, 90 ℃ and any value in between. According to a preferred embodiment of the present application, in step S1, the temperature of the extraction is between 75 and 80 ℃.
According to some embodiments of the present application, in step S1, the time of the extraction is 1-3h, for example, 1h, 1.2h, 1.5h, 1.8h, 2h, 2.2h, 2.5h, 2.8h, 3h, and any value therebetween. According to a preferred embodiment of the present application, in step S1, the extraction time is 2-2.5h.
According to some embodiments of the present application, in step S1, the stirring is performed during the extraction. According to a preferred embodiment of the present application, the stirring speed is 150-300r/min, for example 150r/min, 200r/min, 250r/min, 300r/min and any value in between.
According to some embodiments of the present application, in step S2, the extract after the solid-liquid separation is decolorized and sterilized. According to a preferred embodiment of the present application, the decolorization is performed with activated carbon.
In a fourth aspect, the present application provides the use of a plant extract according to the second aspect or a plant extract obtained according to the method of preparation of the third aspect in a skin care product.
According to some embodiments of the present application, the skin care product is a facial mask.
According to some embodiments of the present application, the plant extract is added to the skin care product in an amount of 3-8wt%, for example 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5% and any value in between. According to a preferred embodiment of the present application, the plant extract is added to the skin care product in an amount of 5-6wt%.
The "water" used for preparing the plant extract is not particularly limited in this application, and preferably, one of distilled water, deionized water and purified water may be used for mass production.
In a fifth aspect, the present application provides a mask liquid comprising the plant extract of the second aspect or the plant extract obtained according to the preparation method of the third aspect.
According to some embodiments of the present application, the plant extract is added to the mask liquid in an amount of 3-8wt%, for example, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5% and any value therebetween. According to a preferred embodiment of the present application, the plant extract is added to the mask liquid in an amount of 5 to 6wt%.
According to some embodiments of the present application, the mask liquid comprises the following components in mass percent:
in a sixth aspect, the present application provides a method for preparing the mask liquid according to the fifth aspect, wherein the water, xanthan gum, polyacrylate crosslinked polymer-6, betaine, dipotassium glycyrrhizinate, glycerol, hydrolyzed hyaluronic acid, glyceroglycosides, methylsilanol mannuronate, the plant extract according to the second aspect or the plant extract obtained by the preparation method according to the third aspect is mixed with hexanediol to obtain the mask liquid.
According to some embodiments of the present application, the temperature of the mixing is 75-80 ℃, which may be, for example, 75 ℃, 76 ℃, 77 ℃, 78 ℃, 79 ℃, 80 ℃ and any value therebetween.
According to some embodiments of the present application, the mixing time is 10-20min, which may be, for example, 10min, 12min, 14min, 16min, 18min, 20min, and any value therebetween.
According to some embodiments of the present application, the mixing is performed while stirring. According to a preferred embodiment of the present application, the stirring speed is 300-500r/min, for example 300r/min, 350r/min, 400r/min, 450r/min, 500r/min and any value in between.
The plant extract provided by the application has the functions of corrosion prevention, bacteriostasis and synergism, the dosage of the traditional preservative in the cosmetics can be reduced, meanwhile, the traditional preservative is compounded with the hexanediol, the true cosmetics without corrosion prevention addition are realized, the cosmetics are safer, and the allergy rate is reduced.
Detailed Description
The present application will be more fully understood by those skilled in the art from the following detailed description of the present application by way of examples, which are not intended to limit the scope of the present application in any way.
The cortex moutan, origanum vulgare, cinnamon, acanthopanax, peppermint, chicken peppers and garden balsam stem used in the following examples all meet the relevant regulations under each medicinal material item of a text of Chinese pharmacopoeia (2015 edition) and national Chinese herbal medicine assembly. Before feeding, the identification shows that the physical objects of the medicinal materials conform to the names and the quality conforms to the standard.
Preparation of bacterial suspension
Preparing corresponding culture medium, sterilizing with damp heat at 121deg.C (fungus culture medium 108 deg.C) for 30min, and cooling and solidifying in a super clean bench. Respectively picking test strains (such as Escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, saccharomyces cerevisiae, and Aspergillus niger) under aseptic condition, inoculating on corresponding culture medium by streak method, and culturing in incubator for activation. Bacteria were cultured at 37℃for 24 hours and fungi were cultured at 28℃for 48-72 hours. Taking the activated fungus lawn of the five bacteria, culturing the bacteria in a corresponding liquid culture medium at 37 ℃ for 24 hours, and culturing the fungi at 28 ℃ for 48-72 hours. The bacteria, mixed bacteria and Saccharomyces cerevisiae are prepared into a bacterial suspension with the concentration of 10 by using sterile physiological saline 8 cfu/ml, mold spore concentration was adjusted to 10 6 cfu/ml, and placing in a refrigerator at 4 ℃ for standby.
All of the above operations were performed on a clean bench.
(1) The experimental method of the inhibition zone comprises the following steps:
the antibacterial activity of the extract is measured by adopting a cup and disc method. Pouring nutrient agar culture medium into the culture dish, and respectively sucking the culture medium to 10% after solidification 8 0.1mL of CFU/mL escherichia coli and staphylococcus aureus suspension is uniformly coated on a flat plate, 3 sterile oxford cups are placed on the flat plate at equal intervals, 200 mu L of traditional Chinese medicine extracting solution is added into each oxford cup, absolute ethyl alcohol is used as a reference, and 3 parallel tests are carried out on each traditional Chinese medicine extracting solution. Culturing at 37deg.C for 18-24 hr, and measuring and recording the diameter of the inhibition zone.
(2) Corrosion challenge experiment
With reference to the CTFA method, 3 parts of 10g of the sample (mask solution) were taken per group, and 0.1ml of the buffer bacterial suspension, 0.1ml of the Saccharomyces cerevisiae suspension and 0.1ml of the Aspergillus niger suspension were added thereto, respectively, so that the initial concentration of the mixed bacteria after inoculation was 10 6 cfu/ml, saccharomyces cerevisiae initial concentration of 10 4 cfu/g, initial concentration of Aspergillus niger of 10 4 cfu/g。
The inoculated samples were placed in 37 ℃ (bacteria) and 28 ℃ (fungi) incubators for culture, and the bacterial content of the samples was measured by dilution coating on days 0, 2, 7, 14, 21 and 28 after inoculation, and each sample was subjected to three parallel experiments and the average value was counted.
Evaluation criteria: the number of fungal colonies decreased 90% on day 7 (i.e., log decrease of 1), the number of bacterial colonies decreased 99.9% (i.e., log decrease of 3), and continued to decrease during the 28 day trial.
All operations were performed on a clean bench.
Example 1
A composition for preparing plant extract with antiseptic and antibacterial effects comprises cortex moutan 2 weight parts, herba Origani 2 weight parts, cortex Cinnamomi 2 weight parts, radix Acanthopanacis Senticosi 3 weight parts, herba Menthae 1 weight parts, fructus Zanthoxyli 1 weight part and herba Speranskiae Tuberculatae 5 weight parts.
The plant extract is prepared by adopting the composition, and the specific steps are as follows:
s1: mixing and coarsely pulverizing the above composition, adding an extraction solvent according to a feed-liquid ratio of 1:15, wherein the extraction solvent is a mixed solution of propylene glycol and water (mass ratio of 1:1), stirring and soaking for 1h at 200r/min, heating to 80 ℃, continuously stirring and extracting for 2h at 200r/min, and filtering with 200-mesh filter cloth to obtain coarse filtrate;
s2: adding flocculant ZTC-3H (obtained from WUHan Zhengtian Biotechnology Co., ltd., hereinafter the same) into the crude filtrate obtained in step S1, stirring, standing for 30min, filtering with 200 mesh filter cloth to remove floccules, continuing filtering with plate YB38 (obtained from Shijiafuotai filtration technology Co., ltd., hereinafter the same), fine filtering to obtain clear liquid, and sterilizing (heating at 85deg.C for 45 min) to obtain target extractive solution.
The prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Example 2
A composition for preparing plant extract with antiseptic and antibacterial effects comprises 3 parts by weight of cortex moutan, 4 parts by weight of origanum vulgare, 4 parts by weight of cortex Cinnamomi, 2 parts by weight of radix Acanthopanacis Senticosi, 2 parts by weight of herba Menthae, 2 parts by weight of fructus Zanthoxyli and 2 parts by weight of herba speranskiae tuberculatae.
The plant extract is prepared by adopting the composition, and the specific steps are as follows:
s1: mixing and coarsely pulverizing the above composition, adding an extraction solvent according to a feed-liquid ratio of 1:10, wherein the solvent is a mixed solution of propylene glycol and water (mass ratio of 1:1), stirring and soaking for 1h at 150r/min, heating to 75 ℃, continuously stirring and extracting for 2h at 150r/min, and filtering with 200-mesh filter cloth to obtain coarse filtrate;
s2: adding flocculant ZTC-3H into the crude filtrate obtained in step S1, stirring, standing for 30min, filtering with 200 mesh filter cloth to remove floccule, filtering with YB38 filter plate, fine filtering to obtain clear liquid, and sterilizing (heating at 85deg.C for 45 min) to obtain target extractive solution.
The prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Example 3
A composition for preparing plant extract with antiseptic and antibacterial effects comprises cortex moutan 1 weight part, herba Origani 3 weight parts, cortex Cinnamomi 3 weight parts, radix Acanthopanacis Senticosi 4 weight parts, herba Menthae 3 weight parts, fructus Zanthoxyli 2 weight parts, and herba Speranskiae Tuberculatae 3 weight parts.
The plant extract is prepared by adopting the composition, and the specific steps are as follows:
s1: mixing and coarsely pulverizing the above composition, adding an extraction solvent according to a feed-liquid ratio of 1:15, wherein the solvent is a mixed solution of propylene glycol and water (mass ratio of 1:1), stirring and soaking for 1h at 250r/min, heating to 80 ℃, continuously stirring and extracting for 2h at 250r/min, and filtering with 200-mesh filter cloth to obtain coarse filtrate;
s2: adding flocculant ZTC-3H into the crude filtrate obtained in step S1, stirring, standing for 30min, filtering with 200 mesh filter cloth to remove floccule, filtering with YB38 filter plate, fine filtering to obtain clear liquid, and sterilizing (heating at 85deg.C for 45 min) to obtain target extractive solution.
The prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Example 4
Plant extracts were prepared using the same materials and methods as in example 1, except that the weight ratio of oregano to chicken peppers was 1:0.1.
The prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Example 5
Plant extracts were prepared using the same materials and methods as in example 1, except that the weight ratio of oregano to chicken peppers was 1:0.25.
The prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Example 6
Plant extracts were prepared using the same materials and methods as in example 1, except that the weight ratio of oregano to chicken peppers was 1:1.
The prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Example 7
Plant extracts were prepared using the same materials and methods as in example 1, except that the weight ratio of oregano to chicken peppers was 1:1.5.
The prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Example 8
Plant extracts were prepared using the same materials and methods as in example 1, except that the weight ratio of oregano to chicken peppers was 1:2.
The prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Example 9
Plant extracts were prepared using the same materials and methods as in example 1, except that the weight ratio of oregano to cinnamon was 1:0.5.
the prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Example 10
Plant extracts were prepared using the same materials and methods as in example 1, except that the weight ratio of oregano to cinnamon was 1:1.5.
the prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Example 11
Plant extracts were prepared using the same materials and methods as in example 1, except that the weight ratio of oregano to cinnamon was 1:2.
the prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Example 12
Plant extracts were prepared using the same materials and methods as in example 1, except that the weight ratio of oregano to cinnamon was 1:3.
the prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Comparative example 1
Plant extracts were prepared using the same materials and methods as in example 1, except that oregano was not included in the composition.
The prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Comparative example 2
Plant extracts were prepared using the same materials and methods as in example 1, except that cinnamon was not included in the composition.
The prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Comparative example 3
Plant extracts were prepared using the same materials and methods as in example 1, except that the compositions did not include capsicum frutescens.
The prepared plant extract is used for a plant inhibition zone test, and the experimental results are shown in table 1.
Table 1 results of inhibition zone experiments
Example 13
The mask liquid comprises the following components in percentage by mass:
the preparation method of the mask liquid comprises the following steps: mixing the above components, stirring at 75-80deg.C at 300-500r/min for 10-20min, and cooling to room temperature.
The prepared mask liquid is used for an anti-corrosion challenge test, and the anti-corrosion challenge test results are as follows: the detailed data are presented in table 2, with corrosion challenge.
Table 2 antiseptic challenge test results
| Colony count (cfu/ml) | Day 0 | Day 2 | Day 7 | Day 14 | Day 21 | Day 28 |
| Mixed bacteria | 2.4×10 6 | 2.1×10 2 | 76 | Not detected | Not detected | Not detected |
| Saccharomyces cerevisiae | 2.1×10 4 | 1.9×10 2 | 30 | Not detected | Not detected | Not detected |
| Aspergillus niger | 1.9×10 4 | 1.4×10 2 | 13 | Not detected | Not detected | Not detected |
The foregoing examples of the present application are merely illustrative of the present application and are not intended to limit the embodiments of the present application, and other variations or modifications may be made by those skilled in the art based on the foregoing description, and it is not intended to be exhaustive of all embodiments, and all obvious variations or modifications that come within the scope of the present application are defined by the claims of the present application.
Claims (24)
1. A plant extract with antiseptic and antibacterial effects, which comprises a composition, wherein the composition comprises 1-5 parts by weight of moutan bark, 1-5 parts by weight of origanum vulgare, 1-5 parts by weight of cinnamon, 0.5-5 parts by weight of acanthopanax, 1-5 parts by weight of peppermint, 0.5-5 parts by weight of chicken pepper and 1-5 parts by weight of garden balsam stem; the weight ratio of the origanum vulgare to the chicken peppers is 1: (0.25-1); the weight ratio of the oregano to the cinnamon is 1 (1-2);
the preparation method of the plant extract comprises the following steps:
s1: mixing the composition with a solvent, and extracting to obtain an extracting solution;
s2: carrying out solid-liquid separation on the extracting solution to obtain the plant extract;
in the step S1, the solvent is a mixture of an organic solvent and water;
in step S1, the organic solvent comprises C1-C5 alcohol compounds.
2. The plant extract of claim 1, wherein the weight ratio of oregano to the chicken peppers is 1: (0.5-1).
3. The plant extract according to claim 2, wherein the composition comprises 2-5 parts by weight of moutan bark, 1-3 parts by weight of oregano, 1-3 parts by weight of cinnamon, 0.5-2 parts by weight of acanthopanax, 1-3 parts by weight of peppermint, 0.5-2 parts by weight of zanthoxylum piperitum and 1-3 parts by weight of garden balsam stem.
4. A process for the preparation of a plant extract as claimed in any one of claims 1 to 3, comprising the steps of:
s1: mixing the composition of any one of claims 1-3 with a solvent, and extracting to obtain an extract;
s2: and carrying out solid-liquid separation on the extracting solution to obtain the plant extract.
5. The method according to claim 4, wherein,
in the step S1, the weight ratio of the composition to the solvent is 1 (10-20); and/or
In the step S1, the mixing time is 0.5-3h; and/or
In the step S1, the extraction temperature is 60-90 ℃; and/or
In the step S1, the extraction time is 1-3h; and/or
In the step S1, stirring is carried out during the extraction; and/or
In step S2, the extract after the solid-liquid separation is decolorized and sterilized.
6. The method according to claim 5, wherein in the step S1, the mixing time is 1 to 2 hours.
7. The method according to claim 5, wherein the extraction temperature is 75-80 ℃ in step S1.
8. The method according to claim 5, wherein in step S1, the extraction time is 2 to 2.5 hours.
9. The method according to claim 5, wherein the stirring speed in step S1 is 150-300r/min.
10. The method according to claim 5, wherein in step S1, the mass ratio of the organic solvent to water is (1:2) - (2:1).
11. The method according to claim 5, wherein in step S1, the organic solvent comprises propylene glycol.
12. The method according to claim 5, wherein in step S2, activated carbon is used for decoloring.
13. Use of a plant extract according to any one of claims 1 to 3 or obtained according to the method of preparation of any one of claims 4 to 12 for the preparation of a skin care product.
14. The use according to claim 13, wherein the skin care product is a facial mask.
15. The use according to claim 13, wherein the plant extract is added to the skin care product in an amount of 3-8wt%.
16. The use according to claim 15, wherein the plant extract is added to the skin care product in an amount of 5-6wt%.
17. A mask liquid comprising the plant extract according to any one of claims 1 to 3 or the plant extract obtained by the production method according to any one of claims 4 to 12.
18. The mask liquid according to claim 17, wherein the plant extract is added to the mask liquid in an amount of 3-8wt%.
19. The mask liquid according to claim 18, wherein the plant extract is added to the mask liquid in an amount of 5%.
20. The mask liquid according to claim 18, wherein the mask liquid comprises the following components in percentage by mass:
77.4-90.34% of water;
0.02-0.1% of xanthan gum;
60.1 to 0.3 percent of polyacrylate crosslinked polymer;
1-3% of betaine;
dipotassium glycyrrhizinate 0.02-0.1%;
glycerol 1-6%;
0.02-0.1% of hydrolyzed hyaluronic acid;
1-2% of glyceroglycosides;
1-2% of methyl silanol mannuronate;
a plant extract according to any one of claims 1 to 3 or a plant extract obtained according to the preparation method of any one of claims 4 to 12, 3 to 8%;
0.5-1% of hexanediol.
21. A method for preparing the mask liquid according to claim 20, which comprises mixing the water, xanthan gum, polyacrylate crosslinked polymer-6, betaine, dipotassium glycyrrhizinate, glycerin, hydrolyzed hyaluronic acid, glyceroglucoside, methylsilanol mannuronate, the plant extract according to any one of claims 1 to 3 or the plant extract obtained by the preparation method according to any one of claims 4 to 12 with hexanediol to obtain the mask liquid.
22. The method of claim 21, wherein the mixing is performed at a temperature of 75-80 ℃ for a time of 10-20min.
23. The method of preparing a mask liquid according to claim 22, wherein the mixing is performed while stirring.
24. The method for preparing a mask liquid according to claim 23, wherein the stirring speed is 300-500r/min.
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