CN115449495A - Complex microbial inoculant and semi-coke wastewater treatment method - Google Patents
Complex microbial inoculant and semi-coke wastewater treatment method Download PDFInfo
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- 239000000571 coke Substances 0.000 title claims abstract description 66
- 230000000813 microbial effect Effects 0.000 title claims abstract description 15
- 239000002054 inoculum Substances 0.000 title claims description 6
- 238000004065 wastewater treatment Methods 0.000 title abstract description 8
- 239000002351 wastewater Substances 0.000 claims abstract description 60
- 239000002131 composite material Substances 0.000 claims abstract description 52
- 241000316848 Rhodococcus <scale insect> Species 0.000 claims abstract description 35
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 34
- 239000012266 salt solution Substances 0.000 claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 33
- 241000589519 Comamonas Species 0.000 claims abstract description 31
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 25
- 239000000126 substance Substances 0.000 claims abstract description 23
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 22
- 241000589776 Pseudomonas putida Species 0.000 claims abstract description 22
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 12
- 241000894006 Bacteria Species 0.000 claims description 47
- 239000001963 growth medium Substances 0.000 claims description 25
- 239000000203 mixture Substances 0.000 claims description 25
- 241000589518 Comamonas testosteroni Species 0.000 claims description 11
- 238000011081 inoculation Methods 0.000 claims description 9
- 230000000593 degrading effect Effects 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 8
- 230000001590 oxidative effect Effects 0.000 claims description 8
- 239000002609 medium Substances 0.000 claims description 7
- 241000187562 Rhodococcus sp. Species 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 6
- 150000001875 compounds Chemical class 0.000 description 15
- 150000002989 phenols Chemical class 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 7
- 239000011734 sodium Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 6
- TUAMRELNJMMDMT-UHFFFAOYSA-N 3,5-xylenol Chemical compound CC1=CC(C)=CC(O)=C1 TUAMRELNJMMDMT-UHFFFAOYSA-N 0.000 description 4
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- 238000002360 preparation method Methods 0.000 description 4
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- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
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- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 231100000086 high toxicity Toxicity 0.000 description 2
- 239000010842 industrial wastewater Substances 0.000 description 2
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- 238000007254 oxidation reaction Methods 0.000 description 2
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- 239000012137 tryptone Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- QWBBPBRQALCEIZ-UHFFFAOYSA-N 2,3-dimethylphenol Chemical compound CC1=CC=CC(O)=C1C QWBBPBRQALCEIZ-UHFFFAOYSA-N 0.000 description 1
- XKZQKPRCPNGNFR-UHFFFAOYSA-N 2-(3-hydroxyphenyl)phenol Chemical compound OC1=CC=CC(C=2C(=CC=CC=2)O)=C1 XKZQKPRCPNGNFR-UHFFFAOYSA-N 0.000 description 1
- QTWJRLJHJPIABL-UHFFFAOYSA-N 2-methylphenol;3-methylphenol;4-methylphenol Chemical compound CC1=CC=C(O)C=C1.CC1=CC=CC(O)=C1.CC1=CC=CC=C1O QTWJRLJHJPIABL-UHFFFAOYSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
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- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000031709 bromination Effects 0.000 description 1
- 238000005893 bromination reaction Methods 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 239000003034 coal gas Substances 0.000 description 1
- 239000011280 coal tar Substances 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
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- SOCTUWSJJQCPFX-UHFFFAOYSA-N dichromate(2-) Chemical compound [O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O SOCTUWSJJQCPFX-UHFFFAOYSA-N 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
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- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 1
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
- C02F2101/345—Phenols
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Abstract
The invention discloses a composite microbial inoculum and a treatment method of semi-coke wastewater, and relates to the technical field of wastewater treatment. Aiming at specific semi-coke wastewater, a composite microbial agent is designed, bacillus cereus, pseudomonas putida, rhodococcus and comamonas in the composite microbial agent jointly act to degrade phenolic substances, and an inorganic salt solution provides nutrients for the composite microbial agent.
Description
Technical Field
The invention relates to the technical field of wastewater treatment, and particularly relates to a composite microbial inoculum and a method for treating semi-coke wastewater.
Background
Semi-coke wastewater is also called semi-coke wastewater and is industrial wastewater generated in the processes of low-temperature dry distillation, coal gas purification and coke quenching of coal. The wastewater has complex components, contains a large amount of pollutants which are difficult to degrade and have high toxicity, such as coal tar, phenols, ammonia, polycyclic aromatic hydrocarbon, cyanide and sulfide, and is typical industrial wastewater with high pollution and high toxicity. Wherein, the content of phenols is 6000-15000 mg/L, about 50% of phenol, about 20% of methyl phenol, about 15% of diphenol, and the rest phenols comprise dimethyl phenol and the like.
At present, phenols in semi-coke wastewater are mainly treated by a stripping distillation method, an extraction method, a chemical oxidation method, an adsorption method and the like, however, most of phenols can be removed by these methods, but the phenols still remain.
Disclosure of Invention
The invention mainly aims to provide a composite microbial inoculum and a method for treating semi-coke wastewater, and aims to solve the problem of residual phenolic substances after semi-coke wastewater treatment.
In order to achieve the purpose, the invention provides a composite microbial inoculum for removing phenolic substances in semi-coke wastewater, which comprises composite bacteria, a culture medium and an inorganic salt solution, wherein the composite bacteria comprise bacillus cereus, pseudomonas putida, rhodococcus and comamonas.
Optionally, the mass fractions of the components in the composite bacteria are as follows:
40 to 50 percent of bacillus cereus, 20 to 30 percent of pseudomonas putida, 10 to 20 percent of rhodococcus and 10 to 20 percent of comamonas.
Optionally, the medium comprises LB medium.
Optionally, the inorganic salt solution comprises the following components in concentrations:
Na 2 HPO 4 ·12H 2 O 50～70g/L、KH 2 PO 4 20～30g/L、FeSO 4 ·7H 2 O 2～3mg/L、MnSO 4 ·H 2 O 0.5～0.7mg/L、MgSO 4 ·7H 2 O 0.15～0.2mg/L、CaCl 2 2.5～3mg/L、CuSO 4 0.125～2mg/L、ZnSO 4 0.125～2mg/L、H 3 BO 3 0.125-2 mg/L and (NH) 4 ) 2 SO 4 2～3g/L。
Optionally, the pH of the inorganic salt solution is 7.0 to 7.5.
Optionally, the Rhodococcus is Rhodococcus (Rhodococcus sp.) CH35R1; and/or the presence of a gas in the atmosphere,
the Comamonas is Comamonas testosteroni (Comamonas testostesonii) strain GEN05.
The invention further provides a treatment method of semi-coke wastewater, which adopts the composite microbial inoculum for treatment, and comprises the following steps:
s10, inoculating the composite bacteria to a culture medium, and adding the composite bacteria to semi-coke wastewater to obtain a mixture;
and S20, adding an inorganic salt solution into the mixture, and degrading under an aerobic condition.
Optionally, before step S10, the method further includes:
s11, respectively filtering, extracting, chemically oxidizing or adsorbing the semi-coke wastewater.
Optionally, in step S10, the inoculation amount of the complex bacteria is 0.1% to 0.5%.
Optionally, in step S20, the amount of the inorganic salt solution added is 5% to 10% of the mass of the mixture.
The technical scheme provided by the invention provides a composite microbial inoculum for removing phenolic substances in semi-coke wastewater, which comprises composite bacteria, a culture medium and an inorganic salt solution, wherein the composite bacteria comprise bacillus cereus, pseudomonas putida, rhodococcus and comamonas. Aiming at specific semi-coke wastewater, a composite microbial agent is designed, bacillus cereus, pseudomonas putida, rhodococcus and comamonas in the composite microbial agent jointly act to degrade phenolic substances, and an inorganic salt solution provides nutrients for the composite microbial agent.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the prior art descriptions will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other relevant drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic flow chart of an embodiment of a semi-coke wastewater treatment method according to the present invention.
The implementation, functional features and advantages of the present invention will be further described with reference to the embodiments and the accompanying drawings.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
It should be noted that those whose specific conditions are not specified in the examples were performed according to the conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially. In addition, the meaning of "and/or" appearing throughout includes three juxtapositions, exemplified by "A and/or B", including either A or B or both A and B. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
At present, phenols in semi-coke wastewater are mainly treated by a stripping distillation method, an extraction method, a chemical oxidation method, an adsorption method and the like, however, most of phenols can be removed by these methods, but the phenols still remain.
In view of this, the invention provides a complex microbial inoculant and a method for treating semi-coke wastewater, and aims to solve the problem of residual phenolic substances after semi-coke wastewater treatment.
In order to achieve the purpose, the invention provides a composite microbial inoculum for removing phenolic substances in semi-coke wastewater, which comprises composite bacteria, a culture medium and an inorganic salt solution, wherein the composite bacteria comprise bacillus cereus, pseudomonas putida, rhodococcus and comamonas.
The technical scheme provided by the invention provides a composite microbial inoculum for removing phenolic substances in semi-coke wastewater, which comprises composite bacteria, a culture medium and an inorganic salt solution, wherein the composite bacteria comprise bacillus cereus, pseudomonas putida, rhodococcus and comamonas. Aiming at specific semi-coke wastewater, a composite microbial agent is designed, wherein bacillus cereus, pseudomonas putida, rhodococcus and comamonas in the composite microbial agent act together to degrade phenolic substances, and an inorganic salt solution provides nutrients for the composite microbial agent.
The proportion of each component in the composite bacteria is not limited in the invention, and the components can be correspondingly added according to the practical application, and in the preferred embodiment of the invention, the mass fraction of each component in the composite bacteria is as follows:
40 to 50 percent of bacillus cereus, 20 to 30 percent of pseudomonas putida, 10 to 20 percent of rhodococcus and 10 to 20 percent of comamonas.
Researches show that the four bacteria can better decompose phenolic substances in the semi-coke wastewater in the proportion, and the decomposition is more thorough and efficient.
The source of the above four bacteria is not limited, and preferably, in the embodiment of the present invention, the Rhodococcus is Rhodococcus sp CH35R1, and the strain is used for treating sewage containing 3, 5-dimethylphenol, which can effectively degrade 3, 5-dimethylphenol in sewage, and the treatment method is simple and efficient; the Comamonas is Comamonas testosteroni strain GEN05, and the Comamonas testosteroni strain GEN05 can efficiently degrade 3-methylphenol in sewage under the conditions of room temperature and aerobic conditions.
Preferably, in an embodiment of the present invention, the medium includes LB medium. The LB medium name is lysogen broth from English. The formula is as follows: 10g/L of Tryptone (Tryptone), 5g/L of Yeast extract (Yeast extract) and 10g/L of sodium chloride (NaCl). The culture medium is used for pre-culturing strains, so that the strains are multiplied to meet the use requirements. In the embodiment of the invention, the LB culture medium is placed in a normal temperature state to be shake-cultured to prepare bacterial liquid for use, and can also be dried at a high temperature to prepare powder for use.
In addition, the specific formulation of the inorganic salt solution is not limited in the present invention as long as it can provide nutrients for the complex bacteria, and preferably, in the embodiment of the present invention, the inorganic salt solution includes the following components in concentration:
Na 2 HPO 4 ·12H 2 O 50～70g/L、KH 2 PO 4 20～30g/L、FeSO 4 ·7H 2 O 2～3mg/L、MnSO 4 ·H 2 O 0.5～0.7mg/L、MgSO 4 ·7H 2 O 0.15～0.2mg/L、CaCl 2 2.5～3mg/L、CuSO 4 0.125～2mg/L、ZnSO 4 0.125～2mg/L、H 3 BO 3 0.125-2 mg/L and (NH) 4 ) 2 SO 4 2～3g/L。
After a plurality of experiments and comparisons, the inventor obtains the inorganic salt solution with the formula, can better provide nutrients for the compound bacteria, enables the compound bacteria to decompose phenolic substances, and is more efficient and rapid.
Further, preferably, the pH of the inorganic salt solution is 7.0 to 7.5. For example, the pH value may be 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, etc., and the pH value is more favorable for the growth and metabolism of each strain in the composite bacteria and further for decomposing phenolic substances.
The invention further provides a method for treating semi-coke wastewater, which adopts the composite microbial inoculum to treat, and fig. 1 is a flow schematic diagram of an embodiment of the method for treating semi-coke wastewater. Referring to fig. 1, the method for treating semi-coke wastewater includes the following steps:
s10, inoculating the composite bacteria to a culture medium, and adding the composite bacteria to semi-coke wastewater to obtain a mixture;
preferably, before step S10, the method further includes:
s11, respectively filtering, extracting, chemically oxidizing or adsorbing the semi-coke wastewater.
After the semi-coke wastewater is respectively filtered, extracted, chemically oxidized or adsorbed, solid particles and other impurities in the semi-coke wastewater can be removed, and then other steps are carried out, so that residual phenolic substances can be effectively removed, the treatment time of the semi-coke wastewater is shortened, and the cost of the deep treatment of the semi-coke wastewater is reduced.
The inoculation amount of the complex bacteria is not limited, and is preferably 0.1 to 0.5%, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, and the like, and the inoculation amount can not waste the complex bacteria and can completely decompose the phenolic substances in the semi-coke wastewater.
And S20, adding an inorganic salt solution into the mixture, and degrading under an aerobic condition.
The addition amount of the inorganic salt solution is not limited, and preferably, the addition amount of the inorganic salt solution is 5 to 10 percent of the mass of the mixture. For example, the amount of the compound bacteria may be 5%, 6%, 7%, 8%, 9%, 10%, etc., and the above-mentioned amount of the compound bacteria can make the respective strains grow well and further improve the metabolic efficiency of the phenolic compounds.
The method for treating semi-coke wastewater, which is definitely provided by the method, is convenient to operate, simple and convenient, can be carried out under the aerobic condition at normal temperature, has all the beneficial effects of the complex microbial inoculum, and is not repeated one by one.
The technical solutions of the present invention are further described in detail below with reference to specific embodiments and the accompanying drawings, it being understood that the following embodiments are merely illustrative of the present invention and are not intended to limit the present invention.
Example 1
Complex microbial inoculum
Compound bacteria: 40% of bacillus cereus, 30% of pseudomonas putida, 10% of Rhodococcus and 20% of Comamonas, wherein the Rhodococcus is Rhodococcus (Rhodococcus sp.) CH35R1, and the Comamonas is Comamonas testosteroni strain GEN05;
culture medium: LB culture medium;
inorganic salt solution (pH 7.0): na (Na) 2 HPO 4 ·12H 2 O 50g/L、KH 2 PO 4 30g/L、FeSO 4 ·7H 2 O 2mg/L、MnSO 4 ·H 2 O 0.7mg/L、MgSO 4 ·7H 2 O 0.15mg/L、CaCl 2 3mg/L、CuSO 4 0.125mg/L、ZnSO 4 2mg/L、H 3 BO 3 0.125mg/L and (NH) 4 ) 2 SO 4 30g/L。
Example 2
Complex microbial inoculum
And (3) compound bacteria: bacillus cereus 50%, pseudomonas putida 20%, rhodococcus 20% and Comamonas 10%, rhodococcus being Rhodococcus (Rhodococcus sp.) CH35R1, comamonas being Comamonas testosteroni strain GEN05;
culture medium: LB culture medium;
inorganic salt solution (pH 7.5): na (Na) 2 HPO 4 ·12H 2 O 70g/L、KH 2 PO 4 30g/L、FeSO 4 ·7H 2 O 2mg/L、MnSO 4 ·H 2 O 0.5mg/L、MgSO 4 ·7H 2 O 0.2mg/L、CaCl 2 2.5mg/L、CuSO 4 2mg/L、ZnSO 4 0.125mg/L、H 3 BO 3 2mg/L and (NH) 4 ) 2 SO 4 2g/L。
Example 3
Complex microbial inoculum
Compound bacteria: 45% of bacillus cereus, 25% of pseudomonas putida, 15% of Rhodococcus and 15% of Comamonas, wherein the Rhodococcus is Rhodococcus (Rhodococcus sp.) CH35R1, and the Comamonas is Comamonas testosteroni (Comamonas testosteroni) strain GEN05;
culture medium: LB culture medium;
inorganic salt solution (pH 7.3): na (Na) 2 HPO 4 ·12H 2 O 60g/L、KH 2 PO 4 25g/L、FeSO 4 ·7H 2 O 2.5mg/L、MnSO 4 ·H 2 O 0.6mg/L、MgSO 4 ·7H 2 O 0.18mg/L、CaCl 2 2.7mg/L、CuSO 4 1mg/L、ZnSO 4 1mg/L、H 3 BO 3 1mg/L and (NH) 4 ) 2 SO 4 2.5g/L。
Example 4
Complex microbial inoculum
Compound bacteria: 47% of Bacillus cereus, 22% of Pseudomonas putida, 18% of Rhodococcus rhodochrous and 12% of Comamonas Comamonas, wherein Rhodococcus rhodochrous is CH35R1, and Comamonas Comamonas testosteroni is Comamonas testosteroni strain GEN05;
culture medium: LB culture medium;
inorganic salt solution (pH 7.1): na (Na) 2 HPO 4 ·12H 2 O 65g/L、KH 2 PO 4 23g/L、FeSO 4 ·7H 2 O 2.8mg/L、MnSO 4 ·H 2 O 0.5mg/L、MgSO 4 ·7H 2 O 0.19mg/L、CaCl 2 2.6mg/L、CuSO 4 1.5mg/L、ZnSO 4 0.8mg/L、H 3 BO 3 1.5mg/L and (NH) 4 ) 2 SO 4 2.1g/L。
Example 5
Complex microbial inoculum
And (3) compound bacteria: 41% of bacillus cereus, 29% of pseudomonas putida, 14% of Rhodococcus and 16% of Comamonas, wherein the Rhodococcus is Rhodococcus (Rhodococcus sp.) CH35R1, and the Comamonas is Comamonas testosteroni strain GEN05;
culture medium: LB culture medium;
inorganic salt solution (pH 7.4): na (Na) 2 HPO 4 ·12H 2 O 55g/L、KH 2 PO 4 24g/L、FeSO 4 ·7H 2 O 2.2mg/L、MnSO 4 ·H 2 O 0.7mg/L、MgSO 4 ·7H 2 O 0.19mg/L、CaCl 2 2.9mg/L、CuSO 4 1.1mg/L、ZnSO 4 1.5mg/L、H 3 BO 3 0.7mg/L and (NH) 4 ) 2 SO 4 2.8g/L。
Example 6
The method for treating semi-coke wastewater uses the complex microbial inoculum of example 1:
(1) Sequentially filtering, extracting, chemically oxidizing and adsorbing semi-coke wastewater;
(2) Inoculating the composite bacteria to a culture medium according to the inoculation amount of 0.1%, and adding the composite bacteria to semi-coke wastewater to obtain a mixture;
(3) Adding an inorganic salt solution accounting for 5% of the mass of the mixture into the mixture, and degrading under the aerobic condition.
Example 7
The method for treating semi-coke wastewater uses the complex microbial inoculum of example 2:
(1) Sequentially filtering, extracting, chemically oxidizing and adsorbing semi-coke wastewater;
(2) Inoculating the compound bacteria to a culture medium according to the inoculation amount of 0.5%, and adding the compound bacteria to semi-coke wastewater to obtain a mixture;
(3) Adding an inorganic salt solution accounting for 10 percent of the mass of the mixture into the mixture, and degrading under the aerobic condition.
Example 8
The method for treating semi-coke wastewater uses the complex microbial inoculum of example 3:
(1) Sequentially filtering, extracting, chemically oxidizing and adsorbing semi-coke wastewater;
(2) Inoculating the compound bacteria to a culture medium according to the inoculation amount of 0.3%, and adding the compound bacteria to semi-coke wastewater to obtain a mixture;
(3) Adding an inorganic salt solution with the mass of 8 percent of the mixture into the mixture, and degrading under the aerobic condition.
Example 9
The method for treating semi-coke wastewater uses the composite microbial inoculum of example 4:
(1) Sequentially filtering, extracting, chemically oxidizing and adsorbing semi-coke wastewater;
(2) Inoculating the composite bacteria to a culture medium according to the inoculation amount of 0.2%, and adding the composite bacteria to semi-coke wastewater to obtain a mixture;
(3) Adding an inorganic salt solution accounting for 7 percent of the mass of the mixture into the mixture, and degrading under the aerobic condition.
Example 10
The method for treating semi-coke wastewater uses the complex microbial inoculum of example 5:
(1) Sequentially filtering, extracting, chemically oxidizing and adsorbing semi-coke wastewater;
(2) Inoculating the compound bacteria to a culture medium according to the inoculation amount of 0.4%, and adding the compound bacteria to semi-coke wastewater to obtain a mixture;
(3) Adding an inorganic salt solution accounting for 6 percent of the mass of the mixture into the mixture, and degrading under the aerobic condition.
Comparative example 1
The components and preparation steps were the same as in example 6, except that no bacillus cereus was added, the mass ratio of pseudomonas putida, rhodococcus and comamonas was 3.
Comparative example 2
The components and preparation steps were the same as in example 6, except that no pseudomonas putida was added, the mass ratio of bacillus cereus, rhodococcus and comamonas was 4.
Comparative example 3
The components and preparation steps were the same as in example 6, except that no rhodococcus was added, the mass ratio of pseudomonas putida, bacillus cereus and comamonas was 4.
Comparative example 4
The components and preparation steps were the same as in example 6, except that no comamonas, pseudomonas putida, bacillus cereus and rhodococcus were added at a mass ratio of 4.
The semi-coke wastewater of two factories is taken as a test object, and the total phenol content and the volatile phenol content in the semi-coke wastewater of the two factories are respectively 900mg/L and 600mg/L, and the following tests are carried out:
the total phenol content, the volatile phenol content, the water ammonia nitrogen content and the Chemical Oxygen Demand (COD) of the semi-coke wastewater are respectively measured before and after the semi-coke wastewater is respectively treated by the methods of examples 6 to 10 and comparative examples 1 to 4 to obtain a table 1, wherein the total phenol content and the volatile phenol content are measured by the national standard of volumetric bromination for measuring water volatile phenol (standard number: HJ 502-2009); the determination of the ammonia nitrogen in the water quality adopts the national standard ' determination of the ammonia nitrogen in the water quality ' Nashin reagent spectrophotometry ' (the standard number is HJ 535-2009); chemical Oxygen Demand (COD) is determined by the dichromate method for determining water quality chemical oxygen demand (standard number: HJ 828-2017).
TABLE 2 measurement results (mg/L) of examples 6 to 10 and comparative examples 1 to 4
As can be seen from table 1, after the treatment of the semi-coke wastewater treatment methods of embodiments 6 to 10 of the present invention, the ammonia nitrogen and the chemical oxygen demand COD of the semi-coke wastewater are both significantly reduced, and the total phenol and volatile phenol contents are not residual, and the treatment is relatively efficient, while comparative examples 1 to 4, in which one strain is absent in the composite bacteria, have significantly poor treatment effects, it can be seen that in the embodiments of the present invention, bacillus cereus, pseudomonas putida, rhodococcus and comamonas are absent, and the joint action of the four strains has an obvious effect on removing phenolic substances in the semi-coke wastewater.
In summary, the composite microbial inoculum provided by the invention is designed aiming at specific semi-coke wastewater, the bacillus cereus, the pseudomonas putida, the rhodococcus and the comamonas act together in the composite microbial inoculum to degrade phenolic substances, and the inorganic salt solution provides nutrients for the composite microbial inoculum.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all equivalent structural changes made by using the content of the present specification or other related technical fields within the spirit of the present invention are included in the scope of the present invention.
Claims (10)
1. The composite microbial inoculum is characterized by being used for removing phenolic substances in semi-coke wastewater, and comprises composite bacteria, a culture medium and an inorganic salt solution, wherein the composite bacteria comprise bacillus cereus, pseudomonas putida, rhodococcus and comamonas.
2. The composite bacterial agent of claim 1, wherein the mass fraction of each component in the composite bacterial agent is as follows:
40 to 50 percent of bacillus cereus, 20 to 30 percent of pseudomonas putida, 10 to 20 percent of rhodococcus and 10 to 20 percent of comamonas.
3. The complex bacterial agent of claim 1, wherein said medium comprises LB medium.
4. The complex microbial inoculant according to claim 1, wherein the inorganic salt solution comprises the following concentrations of components:
Na 2 HPO 4 ·12H 2 O 50～70g/L、KH 2 PO 4 20～30g/L、FeSO 4 ·7H 2 O 2～3mg/L、MnSO 4 ·H 2 O 0.5～0.7mg/L、MgSO 4 ·7H 2 O 0.15～0.2mg/L、CaCl 2 2.5～3mg/L、CuSO 4 0.125～2mg/L、ZnSO 4 0.125～2mg/L、H 3 BO 3 0.125-2 mg/L and (NH) 4 ) 2 SO 4 2～3g/L。
5. The complex microbial inoculant according to claim 1, wherein the inorganic salt solution has a pH of 7.0-7.5.
6. The complex microbial inoculant according to claim 1, wherein the Rhodococcus is Rhodococcus (Rhodococcus sp.) CH35R1; and/or the presence of a gas in the atmosphere,
the Comamonas is Comamonas testosteroni (Comamonas testostesonii) strain GEN05.
7. A method for treating semi-coke wastewater, which is characterized by adopting the composite microbial inoculum of any one of claims 1 to 6, and comprises the following steps:
s10, inoculating the composite bacteria to a culture medium, and adding the composite bacteria to semi-coke wastewater to obtain a mixture;
and S20, adding an inorganic salt solution into the mixture, and degrading under an aerobic condition.
8. The method for treating semi-coke wastewater according to claim 7, further comprising, before step S10:
s11, respectively filtering, extracting, chemically oxidizing or adsorbing the semi-coke wastewater.
9. The method for treating semi-coke wastewater according to claim 7, wherein the inoculation amount of the composite bacteria is 0.1% to 0.5% in step S10.
10. The method for treating semi-coke wastewater according to claim 7, wherein in step S20, the amount of the inorganic salt solution added is 5 to 10% of the mass of the mixture.
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