CN115449361A - Microbial oil displacement agent for high-temperature high-salinity oil reservoir and preparation method thereof - Google Patents
Microbial oil displacement agent for high-temperature high-salinity oil reservoir and preparation method thereof Download PDFInfo
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- 238000006073 displacement reaction Methods 0.000 title claims abstract description 38
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- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
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Abstract
The invention belongs to the technical field of microbial oil displacement agents, and relates to a microbial oil displacement agent for a high-temperature and high-salinity oil reservoir and a preparation method thereof, wherein the microbial oil displacement agent is prepared from the following raw materials in percentage by weight: 20-25% of salt-containing industrial wastewater; 5% -10% of phosphorus-containing industrial wastewater; 10% -18% of bacillus strain; 0.5% -3% of protein; 2-10% of nano magnetic powder compound; the water balance and the nanometer magnetic powder compound can obviously improve the growth rate of microorganisms, provide an adsorption surface for dominant bacteria, further promote the growth and metabolism of the microorganisms and have the effect of strengthening the oil displacement of the microorganisms.
Description
Technical Field
The invention belongs to the technical field of microbial oil displacement agents, and particularly relates to a microbial oil displacement agent for a high-temperature high-salinity oil reservoir and a preparation method thereof.
Background
Along with the development of high-temperature, high-salt and low-permeability oil fields and the successive entering of partial oil wells into a high-water-content period in China, the problems of water control, oil stabilization and water drive improvement of the low-permeability oil field rich in cracks are increasingly urgent; the principle of the widely applied chemical oil displacement technology at present is that chemical recovery aids such as surfactants, polymers and the like are utilized to reduce the viscosity of thick oil, and the method has obvious effect of improving the recovery efficiency, but has the defects of stratum destruction, environmental pollution and the like. In order to overcome the defects of the chemical oil displacement technology, people improve the water displacement operation efficiency and the crude oil recovery rate by utilizing a microbial oil recovery (MEOR) technology, and the MEOR technology becomes a tertiary oil recovery technology with the most development prospect at present.
The microbial oil displacement technology is to inject specific microbe colony and nutrients into water injection well stratum to produce oil displacing matter favorable to raising oil recovering rate, such as gas, organic acid, surfactant, biopolymer, alcohol and other metabolite, and these can lower the interface tension of rock-oil-water system, form oil-in-water emulsion, lower the viscosity of crude oil and improve the flowability of crude oil to raise the oil recovering rate. The microbial oil recovery technology has the advantages of less damage to the environment, no toxicity, harmlessness, high safety, no damage to the health of workers, no damage to the stratum, wide raw material source, economy and environmental protection. However, general microbial strains are restricted by stratum environment for carrying out microbial oil displacement in the oil reservoirs, and many oil reservoirs with the depth of thousands of meters are often high in temperature (above 90 ℃) and high in mineralization (above 15%), microbial oil extraction is limited, so that the strains are required to have high-efficiency strains with the characteristics of temperature resistance and salt tolerance, but the existing microbial strains cannot meet the requirements, and therefore, the problem of high temperature resistance and high mineralization resistance of the microbial strains in the stratum is a key for further improving the microbial oil displacement technology.
Disclosure of Invention
The invention aims to provide a microbial oil displacement agent for a high-temperature and high-salinity oil reservoir, and provides a microbial oil displacement agent which uses a strain mainly for displacing oil from strata and uses salt-containing wastewater and chemical industry wastewater as main production raw materials, so that the production cost is saved, corresponding wastewater can be comprehensively utilized, waste is turned into wealth, and the environment is protected; the microbial oil displacement agent for the high-temperature and high-salinity oil deposit and the preparation method solve the problems that the existing microbial oil displacement strain is low in tolerance of high temperature and high salinity, is restricted by stratum environment, influences oil displacement effect, is high in raw material price and cannot be produced and applied on a large scale.
The technical purpose of the invention is realized by the following technical scheme: a microbial oil displacement agent for high-temperature and high-salinity oil reservoirs is prepared from the following raw materials in percentage by weight: 20-25% of salt-containing industrial wastewater; 5 to 10 percent of phosphorus-containing industrial wastewater; 10% -18% of bacillus strain; 0.5% -3% of protein; 2-10% of nano magnetic powder compound; the balance of water.
The invention is further configured as follows: the nano magnetic powder compound is a compound of nano magnetic ferroferric oxide particles and graphene oxide.
By adopting the technical scheme, the problem of microorganism agglomeration and expansion under the micro-oxygen condition can be solved by adding the magnetic powder, the stimulation effect on the growth and activity of the microorganism is realized, the existence of the graphene oxide provides an adsorption surface for dominant bacteria, an electronic shuttle is provided for the dominant bacteria with slow growth and propagation, the electronic transmission process is enhanced, the biological productivity is increased, the microorganism oil displacement effect is enhanced, the graphene oxide and the nano magnetic powder are compounded, and the problems of agglomeration of nano materials and cell damage can be solved.
The invention is further provided with: the nanometer magnetic powder compound is prepared by the following steps:
s1: weighing a certain amount of graphene oxide, adding the graphene oxide into water, and carrying out ultrasonic treatment for 1h to obtain a graphene oxide glue solution;
s2: weighing a certain amount of nano magnetic ferroferric oxide particles, mixing the nano magnetic ferroferric oxide particles into water, and carrying out ultrasonic stirring treatment for 1 hour;
s3: mixing the graphene oxide glue solution obtained in the step S1 and the step S2 with the nano magnetic ferroferric oxide particles according to the mass ratio of 1; and then heating and drying the mixture at 60-70 ℃ for 2h to obtain the nano magnetic powder compound.
The invention is further provided with: the salt-containing industrial wastewater is high ammonia nitrogen industrial wastewater, and the salt content is 18-25%; the phosphorus-containing industrial wastewater is phosphorus chemical industry production wastewater, the phosphorus content is 0.5% -1%, and the salt-containing industrial wastewater and the phosphorus-containing industrial wastewater are subjected to biochemical treatment.
The invention is further provided with: the biochemical treatment comprises the following steps:
s1: alkali precipitation reaction: adding CaO into industrial wastewater to adjust the pH value to 7-8, adjusting the pH value to =11-12 by using NaOH solution with the concentration of 30%, performing aeration reaction on the industrial wastewater for 8h, and removing the precipitate and the retained liquid.
S2: anaerobic reaction: the wastewater obtained in the step S1 is introduced from the bottom of the anaerobic reactor, a large amount of sludge beds exist in the anaerobic reactor, and waste liquid passing through the sludge beds is discharged from the top of the anaerobic reactor, wherein the sludge beds activate sludge particles through glucose and wheat bran matched with anaerobic bacteria.
S3: aerobic treatment: and (3) introducing the waste liquid obtained in the step (S2) from the bottom of the aerobic reactor, wherein a large amount of flocculent sludge exists in the aerobic reactor, and discharging the waste liquid passing through the flocculent sludge from the top of the aerobic reactor, wherein the flocculent sludge is activated by glucose and an aerobic biological agent.
A preparation method of a microbial oil displacement agent for a high-temperature high-salinity oil reservoir is characterized by comprising the following steps of:
s1: carrying out biochemical treatment on salt-containing industrial wastewater and phosphorus-containing industrial wastewater;
s2: 2-10% of the biochemically treated industrial wastewater and the nano magnetic powder compound; adding 0.5-3% of protein and water into a fermentation tank according to the weight percentage, adjusting the pH value of the solution to 7.0-7.2, heating to 40-55 ℃, uniformly stirring, heating to 125 ℃, and sterilizing for 25 minutes; cooling to 40-60 deg.C, adding Bacillus strain into the fermentation tank, fermenting at 55-60 deg.C for 48 hr, and cooling to room temperature to obtain the required microbial oil-displacing agent.
The invention has the beneficial effects that:
1. microbial strains cultured by the microbial oil displacement agent for the high-temperature high-salinity oil reservoir can resist the high temperature of the stratum of 121 ℃ and can grow in well fluid with the salinity of 22 percent; the preparation method has the advantages of simple process, strong pertinence and operability, low cost, safety and environmental protection, can utilize the salt-containing and phosphorus-containing wastewater produced by local enterprises as production raw materials, and has low production cost and high economic benefit; the problems of large dosage, high cost, environmental pollution and oil product damage caused by the use of the traditional chemical oil displacement agent are solved.
2. According to the invention, the problem of microbial agglomeration and expansion under the micro-oxygen condition can be solved by adding the magnetic powder, the magnetic powder has a stimulation effect on the growth and activity of microbes, the existence of the graphene oxide provides an adsorption surface for dominant bacteria, an electron shuttle is provided for the dominant bacteria with slow growth and reproduction, the loss of the dominant bacteria is reduced, the electron transfer process is enhanced, the biological productivity is increased, the growth and metabolism of the microbes are further promoted, the microbial oil displacement effect is enhanced, the graphene oxide and the nano magnetic powder are compounded, and the problems of agglomeration of nano materials and cell damage can be solved. Meanwhile, the emulsification and viscosity reduction effects of the microorganisms and the metabolites thereof on the residual oil are also enhanced.
3. In the invention, the alkaline precipitation is mainly used for adjusting the pH value of the wastewater to overflow a large amount of ammonia gas, removing most ammonium ions in the wastewater and reducing the pressure of a subsequent biochemical treatment system. The biochemical treatment adopts an anaerobic and aerobic process, the anaerobic process is used as a first treatment process of a biochemical system, pollutants with higher concentration can be borne, organic matters which are difficult to degrade in the wastewater can be well pretreated, and released phosphorus can provide nutrient substances for subsequent aerobic reaction; most of ammonium salt in the wastewater can be removed by an aerobic process.
Detailed Description
The technical solutions in the examples will be clearly and completely described below. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without any inventive step, are within the scope of the present invention.
Comparative example:
20-25% of salt-containing industrial wastewater; 5 to 10 percent of phosphorus-containing industrial wastewater; 10% -18% of bacillus strain; 0.5% -3% of protein; adding water into a fermentation tank according to the weight percentage, adjusting the pH value of the solution to 7.0-7.2, heating to 40-55 ℃, uniformly stirring, and then heating to 125 ℃ for sterilization for 25 minutes; cooling to 40-60 ℃, adding bacillus strains into the fermentation tank according to the weight percentage, fermenting for 48 hours at 55-60 ℃, and cooling to normal temperature to obtain the required microbial oil displacement agent.
Example 1:
20-25% of salt-containing industrial wastewater; 5 to 10 percent of phosphorus-containing industrial wastewater; 10% -18% of bacillus strain; 0.5% -3% of protein; 2-10% of nano magnetic powder compound; adding water into a fermentation tank according to the weight percentage, adjusting the pH value of the solution to 7.0-7.2, heating to 40-55 ℃, uniformly stirring, and then heating to 125 ℃ for sterilization for 25 minutes; cooling to 40-60 deg.c, adding bacillus strain in the fermentation tank, fermenting at 55-60 deg.c for 48 hr, and cooling to normal temperature to obtain the required microbial oil displacing agent
Example 2
S1: performing biochemical treatment on 20-25% of salt-containing industrial wastewater and 5-10% of phosphorus-containing industrial wastewater;
s2: biochemically treating the industrial wastewater; adding 0.5-3% of protein and water into a fermentation tank according to the weight percentage, adjusting the pH value of the solution to 7.0-7.2, heating to 40-55 ℃, uniformly stirring, heating to 125 ℃, and sterilizing for 25 minutes; cooling to 40-60 deg.C, adding Bacillus strain into the fermentation tank, fermenting at 55-60 deg.C for 48 hr, and cooling to room temperature to obtain the required microbial oil-displacing agent.
Example 3
S1: performing biochemical treatment on 20-25% of salt-containing industrial wastewater and 5-10% of phosphorus-containing industrial wastewater;
s2: biochemically treating the industrial wastewater; 2-10% of a nano magnetic powder compound; adding 0.5-3% of protein and water into a fermentation tank according to weight percentage, adjusting the pH value of the solution to 7.0-7.2, heating to 40-55 ℃, uniformly stirring, heating to 125 ℃, and sterilizing for 25 minutes; cooling to 40-60 deg.C, adding Bacillus strain into the fermentation tank, fermenting at 55-60 deg.C for 48 hr, and cooling to room temperature to obtain the required microbial oil-displacing agent.
Table 1 shows the performance indexes of the microbial oil displacement agents in the comparative example and examples 1 to 3
Compared with the comparative example, in the example 1, after the nano magnetic powder compound is added, the growth rate of the microorganism is obviously improved, because the existence of the graphene oxide provides an adsorption surface for the dominant bacteria, an electronic shuttle is provided for the dominant bacteria which grow and propagate slowly, the loss of the dominant bacteria is reduced, the electronic transfer process is enhanced, the biological energy production speed is increased, the growth and metabolism of the microorganism are further promoted, and the effect of enhancing the oil displacement of the microorganism is achieved, in the example 2, compared with the comparative example, the industrial wastewater in the example 2 is subjected to biochemical treatment and NH (NH) treatment 4 + The excessive content of NH in example 2 causes the reduction of the dissolved oxygen concentration in the water body in the oxidation process, leads to the reduction of the water quality and influences the growth and propagation of microbial strains 4 + The removal rate of the microbial oil displacement agent is improved, but the growth of microorganisms is also obviously improved, and compared with other oil displacement technologies such as conventional chemical oil displacement, the microbial oil displacement technology has the following advantages: the adaptability is strong, and the application range is wide; the cost is low, and the effective period is long; the operation (construction) is simple; good effect (oil increasing, precipitation, slow decreasing and enhanced recovery ratio); the environmental protection performance is good.
Claims (6)
1. A microbial oil displacement agent for high-temperature hypersalinity oil reservoirs is characterized in that:
the composite material is prepared from the following raw materials in percentage by weight: 20% -25% of salt-containing industrial wastewater; 5% -10% of phosphorus-containing industrial wastewater; 10% -18% of bacillus strain; 0.5% -3% of protein; 2-10% of nano magnetic powder compound; the balance of water.
2. The microbial oil displacement agent for the high-temperature hypersalinity oil reservoir according to claim 1, which is characterized in that: the nano magnetic powder compound is a compound of nano magnetic ferroferric oxide particles and graphene oxide.
3. The microbial oil displacement agent for the high-temperature hypersalinity oil reservoir according to claim 1, which is characterized in that: the nanometer magnetic powder compound is prepared by the following steps:
s1: weighing a certain amount of graphene oxide, adding the graphene oxide into water, and carrying out ultrasonic treatment for 1h to obtain a graphene oxide glue solution;
s2: weighing a certain amount of nano magnetic ferroferric oxide particles, mixing the nano magnetic ferroferric oxide particles into water, and performing ultrasonic stirring treatment for 1 hour;
s3: mixing the graphene oxide glue solution obtained in the step S1 and the step S2 with the nano magnetic ferroferric oxide particles according to the mass ratio of 1; and then heating and drying the mixture at 60-70 ℃ for 2h to obtain the nano magnetic powder compound.
4. The microbial oil displacement agent for the high-temperature hypersalinity oil reservoir according to claim 1, which is characterized in that: the salt-containing industrial wastewater is high ammonia nitrogen industrial wastewater, and the salt content is 18-25%; the phosphorus-containing industrial wastewater is phosphorus chemical industry production wastewater, the phosphorus content is 0.5% -1%, and the salt-containing industrial wastewater and the phosphorus-containing industrial wastewater are subjected to biochemical treatment.
5. The microbial oil displacement agent for the high-temperature hypersalinity oil reservoir according to claim 1, which is characterized in that: the biochemical treatment comprises the following steps:
s1: alkali precipitation reaction: adding CaO into industrial wastewater to adjust the pH value to be within 7-8, adjusting the pH value to be 11-12 by using a NaOH solution with the concentration of 30 percent, carrying out aeration reaction on the industrial wastewater for 8h, and removing a precipitate and a reserved liquid;
s2: anaerobic reaction: introducing the wastewater obtained in the step S1 from the bottom of the anaerobic reactor, wherein a large amount of sludge beds exist in the anaerobic reactor, and the waste liquid passing through the sludge beds is discharged from the top of the anaerobic reactor, wherein the sludge beds activate sludge particles through glucose and wheat bran matched with anaerobic bacteria;
s3: aerobic treatment: and (3) introducing the waste liquid obtained in the step (S2) from the bottom of the aerobic reactor, wherein a large amount of flocculent sludge exists in the aerobic reactor, and discharging the waste liquid passing through the flocculent sludge from the top of the aerobic reactor, wherein the flocculent sludge is activated by glucose and an aerobic biological agent.
6. The preparation method of the microbial oil displacement agent for the high-temperature and high-salinity oil reservoir according to claim 1, characterized by comprising the following steps:
s1: carrying out biochemical treatment on salt-containing industrial wastewater and phosphorus-containing industrial wastewater;
s2: 2-10% of the biochemically treated industrial wastewater and the nano magnetic powder compound; adding 0.5-3% of protein and water into a fermentation tank according to weight percentage, adjusting the pH value of the solution to 7.0-7.2, heating to 40-55 ℃, uniformly stirring, heating to 125 ℃, and sterilizing for 25 minutes; cooling to 40-60 deg.C, adding Bacillus strain into the fermentation tank, fermenting at 55-60 deg.C for 48 hr, and cooling to room temperature to obtain the required microbial oil-displacing agent.
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Denomination of invention: A microbial oil displacement agent and preparation method for high-temperature and high salinity oil reservoirs Effective date of registration: 20231221 Granted publication date: 20231010 Pledgee: Jingzhou Financing Guarantee Group Co.,Ltd. Pledgor: HUBEI SANXIONG TECHNOLOGY DEVELOPMENT Co.,Ltd. Registration number: Y2023980073140 |