CN115418333A - Bacillus subtilis ZLP-121 and application thereof - Google Patents
Bacillus subtilis ZLP-121 and application thereof Download PDFInfo
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- CN115418333A CN115418333A CN202211098497.6A CN202211098497A CN115418333A CN 115418333 A CN115418333 A CN 115418333A CN 202211098497 A CN202211098497 A CN 202211098497A CN 115418333 A CN115418333 A CN 115418333A
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- bacillus subtilis
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Abstract
The invention relates to a Bacillus subtilis ZLP-121 with the preservation number of CGMCC No.25253, which has stronger control effect on lepidoptera pests, hemiptera pests, coleopteran pests, orthoptera pests, cockroaches, ants and centipede larvae; it has obvious antagonistic action on various plant pathogenic bacteria, aquatic pathogenic bacteria, candida albicans, staphylococcus aureus, escherichia coli and other human pathogenic bacteria; meanwhile, the plant growth can be remarkably promoted, and the application prospect in the development of biological preparations such as biological insecticides, biological pesticides, biological fertilizers, biological seed coatings and the like is good.
Description
Technical Field
The invention relates to a bacillus subtilis ZLP-121 and application thereof.
Background
The plant diseases and insect pests bring serious loss to agricultural production, and the conventional chemical pesticide control not only kills a large amount of natural enemies and beneficial microorganisms and destroys ecological balance, but also causes the drug resistance of the pests to rise sharply and causes great harm to the environment and human health. Therefore, research and development of safe, efficient and environment-friendly products and chemical fertilizer and pesticide substitution technologies have become strategic demands for guaranteeing grain safety, food safety and environmental safety.
The bacillus subtilis is aerobic bacillus producing bacillus rod, and has the features of no toxicity, no harm to human body and animal, environment friendship, high stress resistance, fast propagation speed, etc. and can secrete various active matters. Researches show that the microorganisms have strong inhibition effects on botrytis cinerea, rhizoctonia solani, fusarium wilt, early blight, leaf mold, black spot pathogen, colletotrichum, downy mildew, cladosporium cucumerinum, ring rot, phytophthora, curvularia lunata, acid rot, fusarium graminearum, small leaf spot and the like, and can promote the growth of crops.
However, no relevant reports are found about strains which have the functions of preventing diseases, killing insects and promoting plant growth at the same time.
Disclosure of Invention
The invention aims to provide bacillus subtilis ZLP-121 with efficient insecticidal activity and application thereof.
The invention adopts the following technical scheme:
a Bacillus subtilis ZLP-121 with preservation number of CGMCC No.25253 and preservation unit of common microorganism center of China Committee for culture Collection of microorganisms at the institute of microbiology of China, institute of academy of sciences No. 3, north Chen Xilu No. 1, chao Yangchong, beijing, china, with preservation date of 2022 years, 7 months and 8 days.
An application of the Bacillus subtilis ZLP-121 in preventing and controlling Lepidoptera pests, hemiptera pests, coleoptera pests, orthoptera pests or cockroaches, ants and centipede larvae.
Wherein the lepidoptera pests include diamondback moths, beet armyworms, prodenia litura and cabbage caterpillars.
Wherein the hemiptera pests comprise trialeurodes vaporariorum and cicada pallidiflora.
Wherein the coleopteran pests include wireworms, pseudobroomrape and longicorn.
Wherein the insect pests of the order Orthoptera comprise locust, cricket and mole cricket.
An application of the Bacillus subtilis ZLP-121 in preventing and treating phytopathogen, aquatic pathogenic bacteria and human pathogenic bacteria.
Wherein the plant pathogenic bacteria comprise botrytis cinerea, rhizoctonia solani, fusarium wilt, early blight, leaf mold, black spot pathogen, colletotrichum, downy mildew, cladosporium cucumerinum, ring rot, phytophthora, curvularia lunata, acidovorax, gibberellic disease and microsporum.
Wherein the aquatic pathogenic bacteria comprise Aeromonas hydrophila, edwardsiella tarda and Aeromonas veronii.
Wherein the human pathogenic bacteria comprise Candida albicans, staphylococcus aureus and Escherichia coli.
An application of the bacillus subtilis ZLP-121 in preparing a biological control agent, a biological pesticide, a soil remediation agent or a biological fertilizer.
Wherein the biocontrol preparation, the biological pesticide, the soil remediation agent or the biological fertilizer contains bacillus subtilis ZLP-121 thallus and/or fermentation liquor; or surfactin, iturin, camellin or spermidine separated from the fermentation broth.
The pesticide comprises the following components in parts by weight: 23.65 to 76.5 portions of bacillus subtilis ZLP-121 strain raw powder, 14.3 to 19.3 portions of binder, 1.5 to 2.0 portions of dispersant, 2.2 to 2.7 portions of disintegrant, 0.8 to 1.3 portions of wetting agent and 0.95 to 2.55 portions of synergist.
Wherein, the bacillus subtilis ZLP-121 strain raw powder is prepared by the following method: adding 7-13% of beta-cyclodextrin and 5-13% of CaCO by mass of fermentation liquor into the fermentation liquor of bacillus subtilis ZLP-121 3 And 1.5 to 5.5 percent of MgSO 4 Then spray drying is carried out.
Wherein the binder is selected from soluble starch, carboxymethyl cellulose and dextrin.
Wherein the dispersant is selected from sodium lignosulfonate, sodium tripolyphosphate and sodium pyrophosphate.
Wherein the disintegrant is selected from sodium sulfate, sodium dodecyl sulfate, and polysorbate 80.
Wherein the wetting agent is selected from soybean lecithin, sulfonated oil and alkynediol.
Wherein, the used synergist is: silicone copolymer and polyol mixture, polyether modified trisiloxane, ethoxy modified polytrisiloxane and other silicones, synergistic ether, diethyl maleate, triphenyl phosphate and other detoxification enzyme inhibitors, turpentine, tea saponin, natural pericarpium citri reticulatae essential oil, corn germ oil, rapeseed oil, soybean oil, green tangerine peel orange oil, pine oil, chinaberry fruit oil, castor oil, peppermint oil, thyme oil, eucalyptus oil, sesame oil, olive oil and other plant oils, soybean lecithin nonionic surfactant mixture, polyaspartic acid, alkyl polyglycoside, d-limonene, honey, acetone, cinnamaldehyde, terpinen-4-ol, toosendanin, betula, double wound, oozing, and any one or more preferably.
Furthermore, the coating also comprises 0 to 52 weight parts of auxiliary materials.
Wherein the auxiliary material is calcium carbonate.
Further, the pesticide also comprises 0.65-1.25 parts by weight of chemical pesticide, biological pesticide or plant pesticide.
Wherein the chemical pesticide comprises: alanine methyl ester, difenoconazole, procymidone, imported methiocarpus, benzimidazole No. 44, melam, fomei cream, imported blue powder, chlorantraniliprole, lambda-cyhalothrin, spinetoram, chlorbenzuron-cyhalothrin, imidacloprid, deltamethrin, gibberellic acid, indoleacetic acid, indolebutyric acid, adenine, hydroxyenadenine, benzylaminopurine, 24-epibrassinolide, 22,23, 24-epibrassinolide, 28-epihomobrassinolide, 28-homobrassinolide, 14-hydroxybrassinosterol, triacontanol, S-abscisic acid, ascorbic acid, furfurylaminopurine, dihydroporphin iron, allantoin, hypersensitivity protein, tenuagesin, amino-oligosaccharin, lentinan, chitosan, dextransus, oligosaccharins, beta-lupin polypeptide, cholecystatin, milbemycin, sex attractant, nociceptin, apple moth, spodopterocarpus litura, spodoptera litura, and any of the above species.
Wherein the biopesticide comprises: any one of more than 1 of bacillus subtilis, empedobacter brevis, paenibacillus polymyxa, bacillus thuringiensis, bacillus methylotrophicus, bacillus marinus, bacillus firmus, bacillus sphaericus, bacillus cereus, pseudomonas fluorescens, bacillus laterosporus, empedobacter brevis, bacillus licheniformis, bacillus amyloliquefaciens, rhodopseudomonas palustris, rhodospodophilus thiophila, beauveria bassiana, metarhizium anisopliae, trichoderma harzianum, trichoderma reesei, paecilomyces lilacinus, verticillium pachyces, eremothecium, chaetomium cupreum, pythium oligandrum, blattonella virus, polyhedrosis virus, granulosis virus, spinosad, abamectin, and microsporidian lochia grasseri.
Wherein the botanical insecticide comprises: any 1 or more of azadirachtin, matrine, veratrine, nicotine, rotenone, pyrethrin, celastrol, eucalyptol, anise oil, chamaejasmine, triptolide, curcumenol, cnidium lactone, eugenol, physcion, carvacrol, berberine, sterenol, tea saponin, spirocarb, allicin, d-limonene, terpene alcohol, allyl isothiocyanate, pentadecenylphenol acid, tridecylbenzene acid and hydrocinnamone.
A bactericide which comprises a cell or a fermentation broth of Bacillus subtilis ZLP-121.
Further, the bactericide comprises a fermentation liquor of bacillus subtilis ZLP-121 and a carbendazim solution in a volume ratio of 500: 1; the carbendazim solution is prepared by mixing 1g of carbendazim and 600-1000 mL of water.
A bactericide which comprises surfactin, iturin, camellin or spermin isolated from Bacillus subtilis ZLP-121 fermentation broth.
The invention has the beneficial effects that:
the bacillus subtilis ZLP-121 is an aerobic bacillus-producing rod-shaped microorganism, has the characteristics of environmental friendliness, strong stress resistance, high propagation speed and the like, and can secrete various active substances to promote plant growth.
The bacillus subtilis ZLP-121 has strong insecticidal activity, and can effectively kill lepidoptera pests such as diamond back moth, beet armyworm, prodenia litura and cabbage caterpillar, hemiptera pests such as whitefly and cicada spodoptera, coleoptera pests such as golden insect and pangolin, orthoptera pests such as locust, cricket and mole cricket, cockroaches of blattaria, hymenoptera ants and centipedes of centipede.
The bacillus subtilis ZLP-121 has broad-spectrum antibacterial property, and has obvious inhibition effect on various plant pathogenic bacteria such as botrytis cinerea, rhizoctonia solani, fusarium oxysporum, early blight, leaf mold, black spot pathogen, colletotrichum, downy mildew, scab, ring rot, phytophthora, curvularia, acid rot, fusarium graminearum, small spot pathogen and the like.
The bacillus subtilis ZLP-121 has good application prospect in the aspects of developing into functional microbial preparations for killing insects, preventing diseases and the like.
Drawings
FIG. 1 is a photograph of Bacillus subtilis ZLP-121 colony.
FIG. 2 is a picture of Bacillus subtilis ZLP-121 strain.
Detailed Description
The technical solution of the present invention is described in detail below with reference to preferred embodiments. The following examples are only for illustrating and explaining the present invention and do not constitute a limitation to the technical solution of the present invention.
Example 1 isolation and selection of Bacillus subtilis ZLP-121
(1) Sampling soil: collecting 50-100 g of soil samples from cities in Sichuan province of China; packaging into sterilized kraft paper bag, sealing the bag, and recording sampling location, environment and date.
(2) Bacillus isolation and purification: soil samples were isolated by plate dilution on PB medium. The specific operation is as follows: accurately weighing 10g of soil sample, adding into a 90mL 0.9% normal saline conical flask filled with glass beads, and oscillating for 30min by a 180r/min shaking table to uniformly distribute the soil sample to prepare a soil suspension with the concentration of 10 -1 (ii) a Sequentially diluting to a concentration of 10 -5 、10 -6 、10 -7 . The cells were incubated in 80 ℃ constant temperature water bath for 30min, 100. Mu.L each of the dilutions was spread on a PB plate, and the plate was cultured in an inverted incubator at 30 ℃ for 48h. Picking up single bacterial colonies with different forms,and (3) spore staining is used for identification, the bacillus is purified by a scribing method and then transferred to a test tube inclined plane of a PB culture medium, and after culture at 30 ℃ for 48 hours, the bacillus is stored in a refrigerator at 4 ℃ for later use.
(3) Screening of bacterial strain with functions of killing pests and preventing diseases
Screening of insecticidal strains: taking aphids as target organisms, performing liquid shake flask fermentation culture on purified microorganisms, centrifuging fermentation liquor at a rotation speed of 12000r/min for 10min to remove thalli, filtering supernate with a sterile filter membrane of 0.22 mu m to prepare sterile fermentation filtrate, and appropriately diluting the fermentation filtrate. Putting a proper length of broad bean stem leaves into an erlenmeyer flask. Spraying a proper amount of diluent on live aphids and stems and leaves, putting the mixture into a conical flask, and covering the bottleneck with a thin gauze and fixing the bottleneck with a rubber band. And 2d, counting the aphids, and screening out the strains with better insecticidal effect.
Screening strains with disease prevention effects: and (3) primarily screening by using the gray mold of the cucumber as an indicator bacterium by adopting a plate confronting method. Preparing botrytis cinerea pathogenic bacteria growing on a PDA culture medium into a bacterial cake with d =5mm by using a puncher in a sterile operating room, transferring the bacterial cake to the center of a PDA culture medium plate, respectively connecting sterile filtrate of each antagonistic bacterium obtained by primary screening to a position 2.5cm away from the pathogenic bacteria, setting 3 times of repetition, simultaneously taking the plate only containing the pathogenic bacteria cake as a reference, culturing at a constant temperature of 26 ℃, and starting to measure the diameter of a bacterial colony and calculate the average inhibition rate when CK grows over the culture medium. And screening out the bacterial strains with strong antagonistic action on pathogenic bacteria. The bacterial strains with stronger insecticidal and disease-preventing effects are subjected to freezing preservation at minus 80 ℃.
(4) Identification of pesticidal and disease-preventing strains
According to an experimental method in a handbook for identifying common bacteria systems, the screened strains with stronger insecticidal and disease-preventing effects are subjected to morphological, physiological and biochemical identification. The strain was cultured on a PB medium plate at 32. + -. 1 ℃ for 14-16 hours, and it was observed that the colony numbered ZLP-121 was irregular, white, dull, rough in surface, and raised in the center, and the cell body was rod-shaped (as shown in FIGS. 1-2). The physiological and biochemical identification indexes are shown in Table 1.
TABLE 1 physiological and biochemical Properties of Strain ZLP-121
Note: "+" is positive; "-" is negative.
The strain with the number ZLP-121 is determined to be bacillus by morphological observation and physiological and biochemical experiments. Then, molecular biology classification identification is carried out, 16S rDNA sequence analysis is adopted, and the 16S rDNA sequence is amplified by taking the total genome DNA of the extracted strain as a template. The target fragment is amplified by adopting a universal primer 27F/1492R, the amplified product is detected by 1 percent of agarose gel electrophoresis, and the gene sequencing is carried out by adopting a bidirectional sequencing method. And after BLAST sequence homology comparison is carried out on the sequencing result through a GenBank database, the strain with the number of ZLP-121 is identified as Bacillus subtilis and named as Bacillus subtilis ZLP-121.
The bacillus subtilis ZLP-121 is delivered to the common microorganism center of China Committee for culture Collection of microorganisms, the address is the microorganism research institute of China academy of sciences No. 3, xilu No. 1, north Cheng, chaoyang, china, the preservation number is CGMCC No.25253, and the preservation date is 2022, 7 and 8 days.
Example 2 Effect of Bacillus subtilis ZLP-121 on killing Lepidoptera pests, blatta Seu Periplaneta, hymenoptera ants and Centipede larvae
Fermenting and culturing the bacillus subtilis ZLP-121. NB liquid medium is used, and the formula is as follows: 5g/L of beef extract, 10g/L of peptone, 5g/L of NaCl, 10g/L of glucose and pH7.0. Inoculating according to the inoculation amount of 5 percent, and then placing the mixture in a shaking incubator at 180r/min and 32 ℃ for culturing for 48 hours to obtain fermentation liquor.
The fermentation broth of Bacillus subtilis ZLP-121 is diluted to 10 times, 50 times and 100 times and treated with clear water as a control. Preparing medicinal soil, and using clear water to prepare the medicinal soil as a reference. And adding a proper amount of equivalent foodstuff and uniformly stirring. Selecting diamondback moths, prodenia litura, beet armyworm, cockroaches of the order of cabbage worm and cockroach, ants of the order of hymenoptera and centipede larvae with the same body size by a medicinal soil method, and keeping the temperature in an environment at 25-28 ℃ for 24 hours. The number of test insects per treatment was 45, and each treatment was 3 in parallel. After inoculation, the cells are placed in a room for normal-temperature culture, and the mortality is counted after 56 hours, as shown in table 2.
TABLE 2 killing effect of Bacillus subtilis ZLP-121 on Lepidoptera pests and Blatta Seu Periplaneta Blatta
Example 3 pesticidal Effect of Bacillus subtilis ZLP-121 on Hemiptera pests such as whitefly
Bacillus subtilis ZLP-121 was subjected to fermentation culture (same as in example 2).
The fermentation broth was diluted 10, 50, 100 times and treated with clear water as a control. The food used in the trialeurodes vaporariorum insecticidal experiment is tomato leaves, the food used in the isaria cicada insecticidal experiment is sophora flower, the two kinds of foods are soaked in the liquid medicine for 10min and then are dried by using filter paper, the filter paper is taken out and placed in a wide-mouth bottle filled with the filter paper, 2mL of deionized water is added for moisturizing, the wide-mouth bottle is covered by a preservative film, holes are pricked, and 1mL of water is added every 12 h. 50 pests to be tested at each concentration are repeated for 3 times, 50 pests with similar age are selected and placed on a prepared culture dish to starve for 4 hours, tomato tender leaves and sophora flowers treated by the liquid medicine are added respectively, the replacement is carried out once every 12 hours, the culture is carried out overnight in an incubator at 25 ℃, the death heads of the pests are recorded, and the death rate of aphids under different dilution times is calculated.
Mortality = (number of dead insects ÷ number of test insects) × 100%
And (3) determining the mortality rate: after the pests are treated overnight, the pests are touched by a writing brush head, if the pests are not moved, the pests are regarded as dead, and the number of dead pests is counted and recorded. As can be seen from the data results in Table 3, bacillus subtilis ZLP-121 has good control effect on insects of the order Hemiptera.
TABLE 3 insecticidal Effect of Bacillus subtilis ZLP-121 on Hemiptera insects such as whitefly
Example 4 killing Effect of Bacillus subtilis ZLP-121 on Coleoptera larvae
Bacillus subtilis ZLP-121 was subjected to fermentation culture (same as in example 2).
The fermentation liquid of the bacillus subtilis ZLP-121 is diluted to 10 times, 50 times and 100 times and treated by clear water as a contrast. Preparing medicinal soil, and using clear water to prepare the medicinal soil as a reference. And adding a proper amount of equivalent foodstuff and uniformly stirring. Selecting three-instar larvae of golden needle worms, pangolin and mulberrys with the same size by a medicinal soil method, and keeping the larvae at the temperature of 25-28 ℃ for 24 hours. The number of test insects per treatment was 15, and each treatment was performed in 3 replicates, which were repeated 3 times. After inoculation, the cells were cultured at room temperature, and the mortality was counted after 56 hours, as shown in Table 4.
TABLE 4 killing effect of Bacillus subtilis ZLP-121 on Coleoptera larvae such as Flammulina velutipes
Example 5 insecticidal Effect of Bacillus subtilis ZLP-121 on orthoptera pests such as locust, cricket, gryllotalpa, etc
Bacillus subtilis ZLP-121 was subjected to fermentation culture (same as in example 2).
The fermentation broth was diluted to 10, 50, 100 times and treated with clear water as a control. The food used in the locust disinsection experiment is green soy beans, the food used in the cricket disinsection experiment is green soy beans, the food used in the mole cricket disinsection experiment is cabbage leaves, the three foods are soaked in the liquid medicine for 10min and then are dried by filter paper, the three foods are taken out and placed in a wide-mouth bottle filled with the filter paper, 2mL of deionized water is added for moisturizing, the wide-mouth bottle is covered by a preservative film, holes are pricked, and 1mL of water is added every 12 h. And (3) repeating the steps for 30 pests to be tested at each concentration for 3 times, selecting pests with 30 heads and similar big and small sizes, starving the pests on a prepared culture dish for 4 hours, respectively adding the wheat, green soy bean and Chinese cabbage leaves treated by the liquid medicine, replacing the pests once every 12 hours, culturing the pests in an incubator at 25 ℃ overnight, recording the death heads of the pests, and calculating the death rate of the pests under different dilution times.
Mortality = (number of dead insects ÷ number of test insects) × 100%
As can be seen from the results of the data in Table 5, bacillus subtilis ZLP-121 has good control effect on orthoptera insects such as locusts, crickets, mole crickets and the like.
TABLE 5 control Effect of Bacillus subtilis ZLP-121 on Orthoptera insects such as locusts
EXAMPLE 6 determination of the bacterial inhibition Spectrum of Bacillus subtilis ZLP-121
Selecting common pathogenic bacteria such as: a plurality of plant pathogenic bacteria such as tomato leaf mold pathogen, tomato gray mold pathogen, pear black spot pathogen, fusarium, cotton wilt pathogen, cucumber gray mold pathogen, grape gray mold pathogen, cucumber colletotrichum, cucumber downy mildew pathogen, cucumber scab pathogen, apple ring rot pathogen, phytophthora capsici, curvularia, acid rot pathogen, rhizoctonia solani, wheat scab pathogen, corn microsporum pathogen, tomato early blight pathogen and the like; aquatic pathogenic bacteria such as Aeromonas hydrophila, edwardsiella tarda, aeromonas veronii, etc.; pathogenic bacteria such as candida albicans, staphylococcus aureus, escherichia coli and the like are taken as targets, and the bacteriostasis spectrum of the strain ZLP-121 is measured by adopting a plate confronting method to determine the inhibition capacity of the ZLP-121 strain on the growth of the pathogenic bacteria.
As can be seen from the implementation results of Table 6, the strain ZLP-121 has strong antagonistic action on 24 pathogenic bacteria, wherein the inhibition action on Botrytis cinerea is strongest, and the diameter of an inhibition zone can reach 30.77mm. The bactericide which takes the fermentation liquor of the bacillus subtilis ZLP-121 as the active component has good application value in agricultural production.
TABLE 6 inhibitory Effect of Bacillus subtilis ZLP-121 on different phytopathogens
Example 7 preparation of Bacillus subtilis ZLP-121 insecticide (Water dispersible granule)
(1) Preparation of Bacillus subtilis ZLP-121 strain raw powder
NB liquid culture medium is used for culturing the bacillus subtilis ZLP-121, and the formula is as follows: 5g/L beef extract, 10g/L peptone, 5g/L NaCl, 10g/L glucose and pH7.0. Inoculating according to the inoculation amount of 5 percent, and then placing in a shaking incubator at 180r/min and 32 ℃ for culturing for 48h. Spray drying the cultured fermented bacterial liquid of Bacillus subtilis ZLP-121, selecting 11% of beta-cyclodextrin and 9% of CaCO as filler 3 3% MgSO (MgSO) of 4 The inlet temperature is 180 ℃, the feed liquid temperature is set to be normal temperature of 22 ℃, and the wind speed is 30m 3 And/h, feeding the material at a flow rate of 15mL/min, and spray-drying to obtain the bacillus subtilis ZLP-121 strain raw powder.
(2) Process for preparing insecticide
The preparation method of the formula 1 comprises the following steps: 27.6 percent of bacillus subtilis ZLP-121 powder is fully mixed with 52 percent of auxiliary material (calcium carbonate), 14.3 percent of binder soluble starch, 1.5 percent of dispersant sodium lignosulfonate, 2.7 percent of disintegrant sodium sulfate, 0.8 percent of wetting agent soybean lecithin and 1.1 percent of synergist soybean oil, the mixture is crushed by a jet mill and then evenly mixed, then distilled water is added to be kneaded into blocks, the blocks are extruded and granulated by a granulator, the obtained sample is placed in an oven to be dried at the temperature of between 45 and 50 ℃, and the bacillus subtilis ZLP-121 insecticide is obtained by a 40-mesh vibrating screen.
The preparation method of the formula 2 comprises the following steps: 24.90 percent of bacillus subtilis ZLP-121 strain raw powder, 51 percent of auxiliary material (calcium carbonate), 16.3 percent of adhesive carboxymethyl cellulose, 2.0 percent of dispersant sodium tripolyphosphate, 2.2 percent of disintegrant sodium dodecyl sulfate, 1.3 percent of wetting agent sulfonated oil and 2.3 percent of synergist polyether modified trisiloxane are fully mixed, crushed by a jet mill and then uniformly mixed, then distilled water is added to knead the mixture into blocks, a granulator is used for extrusion and granulation, the obtained sample is placed in an oven to be dried at the temperature of 45-50 ℃, and a bacillus subtilis ZLP-121 insecticide is obtained by a 40-mesh vibrating screen.
The preparation method of the formula 3 comprises the following steps: the method comprises the steps of fully mixing 26.25% of bacillus subtilis ZLP-121 strain raw powder with 50% of auxiliary materials (calcium carbonate), 17.3% of binder dextrin, 1.5% of dispersant sodium pyrophosphate, 2.5% of disintegrant polysorbate 80, 1.1% of wetting agent acetylenediol and 1.35% of synergist ethoxy modified polytrisiloxane, crushing by a jet mill, uniformly mixing, adding distilled water, kneading into blocks, extruding and granulating by a granulator, placing the obtained sample in an oven for drying at 45-50 ℃, and screening by a 40-mesh vibrating screen to obtain the bacillus subtilis ZLP-121 pesticide.
The correction insecticidal rates of formula 1, formula 2 and formula 3 on prodenia litura are 76%, 75% and 77%; the corrected insecticidal rates of the golden insects are 74%, 72% and 74%; the corrected insecticidal rates for crickets were 70%, 69% and 70%.
Example 8 preparation of Bacillus subtilis ZLP-121 Fungicide (powder)
The zymophyte liquid of the bacillus subtilis ZLP-121 (same as the example 2) and the carbendazim solution (1 g of carbendazim is dissolved in 800mL of water) are uniformly mixed according to the volume ratio of 500: 1, and then spray drying is carried out to obtain the powder of the bacillus subtilis ZLP-121 bactericide.
Example 9 growth promoting Effect of Bacillus subtilis ZLP-121 Fungicide
Cucumber is selected as a test crop, 20 indoor potted cucumber seedlings are planted in a pot, 3 seedlings are arranged in parallel, a bacillus subtilis ZLP-121 bactericide is applied to a treatment group (example 8), and no production promoting product is applied to a control group. Then, 10 cucumber seedlings of 28 days old are randomly selected, the plant height of the seedlings is measured by a ruler, and the stem thickness is measured by a vernier caliper. Deactivating enzyme at 105 deg.C for 15min, drying at 75 deg.C to constant weight, determining the dry mass of overground part and underground part, determining chlorophyll content by ethanol-acetone extraction, and determining photosynthetic parameters by portable photosynthetic determination system. As can be seen from Table 7, the Bacillus subtilis ZLP-121 bactericide improves the plant height, stem thickness, single overground part fresh quality, single overground part dry quality, leaf area, single plant root fresh quality, single plant root dry quality, single plant total root volume, chlorophyll total amount and net photosynthetic rate of cucumber seedlings by 4.68%, 9.51%, 21.13%, 15.51%, 3.51%, 56.67%, 2.95%, 110.53%, 69.15% and 85.73% compared with CK, and reaches the level of obvious difference except for plant height, leaf area and single plant root dry quality. The bacillus subtilis ZLP-121 bactericide has a remarkable growth promoting effect on cucumber seedlings.
TABLE 7 growth promoting effect of Bacillus subtilis ZLP-121 bactericide on cucumber seedlings
Note: the same letter in the same row represents that P is more than 0.05, the difference is not significant, and different letters represent that P is less than 0.05, and the difference is significant.
EXAMPLE 10 preparation of Bacillus subtilis ZLP-121 insecticides (including insecticides)
According to the mass ratio (see table 8), a bacillus subtilis ZLP-121 insecticide formulation containing a chemical insecticide, a biological insecticide and a botanical insecticide was prepared. Wherein the bacterial powder is the raw powder of the bacillus subtilis ZLP-121 strain obtained in the step (1) of the embodiment 6.
The preparation method comprises the following steps: mixing the raw powder of the bacillus subtilis ZLP-121 strain with auxiliary materials, then fully mixing the raw powder with a binder, a dispersing agent, a disintegrating agent, a wetting agent, an insecticide and a synergist, crushing the mixture by a jet mill, mixing the mixture uniformly, then adding distilled water, kneading the mixture into blocks, performing extrusion granulation by a granulator, placing the obtained samples in an oven to dry at 45-50 ℃, and sieving the dried samples by a 40-mesh vibrating screen to obtain the bacillus subtilis ZLP-121 insecticide.
The auxiliary material is calcium carbonate.
The used binders are: the starch adhesive comprises (1) soluble starch, (2) sodium carboxymethyl cellulose and (3) dextrin.
The dispersants used were: sodium lignosulfonate (A), sodium tripolyphosphate (B) and sodium pyrophosphate (C).
The used disintegrating agents are: (a) Sodium sulfate, (b) sodium lauryl sulfate, and (e) polysorbate 80.
The wetting agents used were: (alpha) soybean lecithin, (beta) sulfonated oil, (gamma) acetylenic diol.
The synergist is as follows: silicone copolymers, (2) polyether-modified trisiloxanes, (3) ethoxy-modified silicones such as polytrisiloxane, and the like, (4) synergistic ethers, (5) diethyl maleate, (6) detoxifying enzyme inhibitors such as triphenyl phosphate, and the like, (7) turpentine, (8) tea saponin, (9) natural dried orange peel essential oil, (10) corn germ oil, (11) rapeseed oil, (12) soybean oil, (13) green tangerine peel oil, (14) pine oil, (15) neem oil, (16) castor oil, (17) peppermint oil, (18) thyme oil, (19) eucalyptus oil, (20) sesame oil, (21) vegetable oils such as olive oil, (22) soybean lecithin nonionic surfactant blend, (23) polyaspartic acid, (24) alkyl polyglycoside, (25) d-limonene, (26) honey, (27) acetone, (28) cinnamaldehyde, (29) terpinen-4-ol, (30) toosendanin, (31) bethan, (32) fenugreek, (33) cyclonite, (34) one or more than one of any one of (1 or 2) preferable.
Chemical insecticides include: alanine methyl ester (1), difenoconazole (2), prochloraz (3), prochloraz (4), imported methimazole (5), benzimidazole No. 44, (6) memylamine, (7) fomesafen (8), imported blue powder, (9) chlorantraniliprole, (10) lambda-cyhalothrin, (11) spinetoram, (12) chlorbenzuron cypermethrin, (13) imidacloprid, (14) deltamethrin, (15) gibberellic acid, (16) indoleacetic acid, (17) indolebutyric acid, (18) enosine, (19) oxprenadenine, (20) benzylaminopurine, (21) 24-epibrassinolide, (22) 22,23, 24-epibrassinolide, (23) 28-epibrassinolide, (24) 28-homobrassinolide, (25) 14-hydroxybrassinosterol, (26) triacontanol, (27) S-abscisin, (28) ascorbyl, (29) allantoin, (30) dihydrobrassinolide, (31) oligogalactan, (39) 34-polyenylidene polysaccharide, (39) polyenylidene glucose-beta-lactonase, 35) polyenylidene polysaccharide, (35) polyenoic acid, (40) Cholecalciferol, (41) milbemycin, (42) Chilo suppressalis sex attractant, (43) prodenia litura sex pheromone, (44) lygus lucorum sex pheromone, (45) pear sex pheromone, and (46) codling moth sex pheromone.
The biological insecticide comprises: any one or more than 1 of bacillus subtilis, (2) Empedobacter brevis, (3) Paenibacillus polymyxa, (4) Bacillus thuringiensis, (5) Bacillus methylotrophicus, (6) Bacillus marinus, (7) Bacillus firmus, (8) Bacillus sphaericus, (9) Bacillus cereus, (10) Pseudomonas fluorescens, (11) Brevibacillus laterosporus, (12) Empedobacter brevis, (13) Bacillus licheniformis, (14) Bacillus amyloliquefaciens, (15) Rhodopseudomonas palustris, (16) Rhodococcus thiophilus, (17) Beauveria bassiana, (18) Beauveria bassiana, (19) Metarhizium anisopliae, (20) Trichoderma harzianum, (21) Trichoderma reesei, (22) Paecilomyces lilacinus, (23) Verticillus pachyces, (24) fungus, (25) Tocophaerotheca minitans, (26) Micropythium minitans, (27) Pythium androgens, (28) Spirovirus, (29) polyhedrosis virus, (30) granulosis virus, (31) spinosad, (32) Abelminth, and (33) Abelminthin.
The plant pesticide comprises: any 1 or more than 2 of nimbin, (2) matrine, (3) veratrine, (4) nicotine, (5) rotenone, (6) pyrethrin, (7) celastrine, (8) eucalyptol, (9) star anise oil, (10) stellerin, (11) triptolide, (12) curcumenol, (13) cnidium lactone, (14) eugenol, (15) physcion, (16) carvacrol, (17) berberine, (18) sterol, (19) tea saponin, (20) spirocarb, (21) allicin, (22) d-limonene, (23) terpene alcohol, (24) allyl isothiocyanate, (25) pentadecenylphenolic acid, (26) tridecanolic acid and (27) hydrocinnamone.
TABLE 8 Bacillus subtilis ZLP-121 insecticide formulations
The embodiments and methods described above are the best embodiments of the present invention, and some changes, modifications, substitutions and combinations can be made without departing from the technical principles of the present invention, and are included in the scope of the present invention.
Claims (10)
1. Bacillus subtilis (B.subtilis)Bacillus subtilis) ZLP-121 with preservation number CGMCC No.25253.
2. Use of the bacillus subtilis ZLP-121 of claim 1 for controlling lepidopteran, hemipteran, coleopteran, orthopteran pests or larvae of cockroaches, ants and centipedes.
3. Use of the bacillus subtilis ZLP-121 according to claim 1 for the control of phytopathogens, aquapathogens and human pathogens.
4. The use according to claim 3, wherein the phytopathogens comprise Botrytis cinerea, rhizoctonia solani, fusarium oxysporum, alternaria solani, phytophthora parasitica, blastomyces nigricans, alternaria, peronospora, phytophthora parasitica, curvularia, phytophthora spp, fusarium oxysporum, microsporum; the aquatic pathogenic bacteria include Aeromonas hydrophila, edwardsiella tarda, and Aeromonas veronii.
5. Use according to claim 3, wherein said human pathogenic bacteria comprise Candida albicans, staphylococcus aureus, escherichia coli.
6. Use of the bacillus subtilis ZLP-121 according to claim 1 for the preparation of a biocontrol agent, a biopesticide, a soil remediation agent, or a biofertilizer.
7. The pesticide is characterized by comprising the following components in parts by weight: 23.65 to 76.5 parts of raw powder of a bacillus subtilis ZLP-121 strain, 14.3 to 19.3 parts of binder, 1.5 to 2.0 parts of dispersant, 2.2 to 2.7 parts of disintegrant, 0.8 to 1.3 parts of wetting agent and 0.95 to 2.55 parts of synergist.
8. The insecticide according to claim 7, wherein said Bacillus subtilis ZLP-121 strain raw powder is prepared by the following method: adding 7-13% of beta-cyclodextrin and 5-13% of CaCO by mass into the fermentation liquor of bacillus subtilis ZLP-121 3 And 1.5 to 5.5 percent of MgSO 4 Then spray drying is carried out.
9. The insecticide according to claim 7, further comprising 0.65 to 1.25 parts by weight of a chemical insecticide, a biological insecticide or a plant insecticide.
10. The bactericide is characterized by comprising bacillus subtilis ZLP-121 thallus, fermentation liquor, or surfactin, iturin, camellin or spermin separated from the fermentation liquor.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116420737A (en) * | 2023-06-08 | 2023-07-14 | 山东京青农业科技有限公司 | Bacillus subtilis water dispersible granule and preparation method and application thereof |
| CN116555078A (en) * | 2023-03-20 | 2023-08-08 | 四川农业大学 | Bacillus subtilis for effectively degrading cypermethrin and application thereof |
| CN116555078B (en) * | 2023-03-20 | 2024-05-31 | 四川农业大学 | Bacillus subtilis for effectively degrading cypermethrin and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105483054A (en) * | 2016-01-04 | 2016-04-13 | 河北省科学院生物研究所 | Bacillus amyloliquefaciens WS-8 and application thereof |
| CN108513988A (en) * | 2018-03-27 | 2018-09-11 | 湖南省蓝正微生物科技开发有限公司 | Microorganism composite pesticide |
| WO2020064480A1 (en) * | 2018-09-28 | 2020-04-02 | Basf Se | Pesticidal mixture comprising a mesoionic compound and a biopesticide |
| CN114717165A (en) * | 2022-05-19 | 2022-07-08 | 河北省科学院生物研究所 | Complex microbial inoculant and biocontrol seed coating agent containing complex microbial inoculant |
| CN114717164A (en) * | 2022-05-19 | 2022-07-08 | 河北省科学院生物研究所 | Bacillus belgii ZLP-101 and application thereof |
| CN114831120A (en) * | 2022-06-16 | 2022-08-02 | 河北省科学院生物研究所 | Lipopeptide-containing composition and application thereof in pest control |
| CN114836351A (en) * | 2022-05-19 | 2022-08-02 | 河北省科学院生物研究所 | Compound microbial agent for killing insects and preventing diseases and biological fertilizer containing microbial agent |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101188641B1 (en) * | 2011-12-13 | 2012-10-08 | 주식회사 지디 | Novel bacillus subtilis gdya-1 having anti-plant pathogens activity and preparation of microbial pesticide |
| CN110079483B (en) * | 2019-05-28 | 2021-07-16 | 黄河三角洲京博化工研究院有限公司 | Bacillus subtilis and application thereof |
| CN110423707B (en) * | 2019-06-27 | 2022-07-22 | 南通大学 | Bacillus subtilis strain NH-6 and application thereof |
-
2022
- 2022-09-08 CN CN202211098497.6A patent/CN115418333A/en active Pending
-
2023
- 2023-08-02 CN CN202310964662.XA patent/CN117535170A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105483054A (en) * | 2016-01-04 | 2016-04-13 | 河北省科学院生物研究所 | Bacillus amyloliquefaciens WS-8 and application thereof |
| CN108513988A (en) * | 2018-03-27 | 2018-09-11 | 湖南省蓝正微生物科技开发有限公司 | Microorganism composite pesticide |
| WO2020064480A1 (en) * | 2018-09-28 | 2020-04-02 | Basf Se | Pesticidal mixture comprising a mesoionic compound and a biopesticide |
| CN114717165A (en) * | 2022-05-19 | 2022-07-08 | 河北省科学院生物研究所 | Complex microbial inoculant and biocontrol seed coating agent containing complex microbial inoculant |
| CN114717164A (en) * | 2022-05-19 | 2022-07-08 | 河北省科学院生物研究所 | Bacillus belgii ZLP-101 and application thereof |
| CN114836351A (en) * | 2022-05-19 | 2022-08-02 | 河北省科学院生物研究所 | Compound microbial agent for killing insects and preventing diseases and biological fertilizer containing microbial agent |
| CN114831120A (en) * | 2022-06-16 | 2022-08-02 | 河北省科学院生物研究所 | Lipopeptide-containing composition and application thereof in pest control |
Non-Patent Citations (1)
| Title |
|---|
| 谢凤形, 天津科技翻译出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116555078A (en) * | 2023-03-20 | 2023-08-08 | 四川农业大学 | Bacillus subtilis for effectively degrading cypermethrin and application thereof |
| CN116555078B (en) * | 2023-03-20 | 2024-05-31 | 四川农业大学 | Bacillus subtilis for effectively degrading cypermethrin and application thereof |
| CN116420737A (en) * | 2023-06-08 | 2023-07-14 | 山东京青农业科技有限公司 | Bacillus subtilis water dispersible granule and preparation method and application thereof |
| CN116420737B (en) * | 2023-06-08 | 2023-10-10 | 山东京青农业科技有限公司 | Bacillus subtilis water dispersible granule and preparation method and application thereof |
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