CN115414423A

CN115414423A - Traditional Chinese medicine composition for treating eczema and application of traditional Chinese medicine composition in preparation of itching-relieving and healing-promoting cream

Info

Publication number: CN115414423A
Application number: CN202211173707.3A
Authority: CN
Inventors: 杨延龙; 陈婷如; 丁卢颖
Original assignee: Second Military Medical University SMMU
Current assignee: Second Military Medical University SMMU
Priority date: 2022-09-26
Filing date: 2022-09-26
Publication date: 2022-12-02
Anticipated expiration: 2042-09-26
Also published as: CN115414423B

Abstract

The invention discloses a traditional Chinese medicine composition for treating eczema, which is prepared from the following components in parts by weight: 5 to 15 parts of golden larch bark, 5 to 15 parts of wild chrysanthemum flower, 5 to 15 parts of garden burnet root, 5 to 15 parts of wrinkled gianthyssop herb, 5 to 15 parts of oriental wormwood, 4 to 10 parts of fineleaf schizonepeta herb, 3 to 8 parts of divaricate saposhnikovia root, 3 to 8 parts of common cnidium fruit, 3 to 8 parts of mint, 1 to 5 parts of amur corktree bark, 1 to 5 parts of myrrh and 3 to 8 parts of liquorice. The itching-relieving and healing-promoting cream preparation is suitable for treating acute, subacute and chronic eczema, has good curative effect, obviously improves erythema, desquamation, lichenification and pruritus of an eczema mouse, can reduce the thickness of the skin of the mouse, reduces the serum IgE level of the mouse and has no obvious adverse reaction.

Description

Traditional Chinese medicine composition for treating eczema and application of traditional Chinese medicine composition in preparation of itching-relieving and healing-promoting cream

Technical Field

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating eczema and application of the traditional Chinese medicine composition in preparation of an itching-relieving and healing-promoting cream.

Background

Eczema (AD) is an inflammatory reaction of skin with severe pruritus caused by various internal and external factors, and with the continuous improvement of the etiology and mechanism thereof in recent years, scholars at home and abroad divide the skin into atopic dermatitis, contact dermatitis and the like. Eczema tends to exude in the acute stage and infiltrates and is hypertrophic in the chronic stage. The skin lesion has the characteristics of polymorphism, symmetry, pruritus, easy repeated attack and the like. The incidence of eczema is on the rise year by year in our country, with the incidence of childhood ranging from 0.7% in 1998 to 12.94% in 2014 (Gu Heng, yan Yan, chen, etc. national atopic dermatitis epidemiological investigation [ J ] Chinese journal of dermatology, 2000,33 (6): 4.; guo Y, li P, tang J, et al. Its pathogenesis is also influenced by various environmental and genetic factors, of which the skin barrier dysfunction and immune dysfunction are the main pathological conditions, which cause repeated inflammation and itching of the skin. AD is known to be primarily immune system dysfunction and environmental factors damaging the skin barrier, exacerbating immunoglobulin E (IgE) -mediated sensitization, severe skin inflammation and immune responses (Giustizieri M L, mascia F, frezzolini A, et al. Keratino microorganisms from tissues with atomic resolution and ps show a diagnosis of chemotherapy process profile in response to T cell-derived cytokines. [ J ]. J Allergy Clin immune, 2001,107 (5): 871-877.).

According to the Chinese guideline for diagnosis and treatment of atopic dermatitis (2020 version) (immunological group of the dermatological society of the Chinese medical society, center for cooperative research on atopic dermatitis, chinese guideline for diagnosis and treatment of atopic dermatitis (2020 version) [ J ]. Chinese journal of dermatology, 2020,053 (002): 81-88.), the purpose of treating AD is to alleviate or eliminate clinical symptoms, reduce and prevent recurrence, improve the quality of life of patients, and enable patients to enjoy normal life. In short, the therapeutic goal of AD is to maintain remission. Therefore, timely and effective reduction of inflammation is a primary goal of treatment.

Existing topical basic anti-inflammatory treatments for eczema include glucocorticoids (TCS), calcineurin inhibitors (TCI) and JAK inhibitors (Mehta a, nadkarni N, patil S, et al. Topical dermatological in informatics [ J ]. Indian Journal of dermatological surgery and Leprology, 8978 zft 8978 (4): 1093-105.). TCS, while effective in controlling inflammation, is susceptible to recurrent attacks of AD, and prolonged use can result in dry, atrophic, hyperpigmented epidermis, and even aggravated infection (Siegfried E C, jaworski J C, kaiser J D, et al. Systematic review of published tertiary; long-term safety of topical cosmetic and topical cosmetic inhibitors in topical patients with topical efficacy of topical cosmetic derivatives [ J ]. Bmc plaques, 2016,16 (1): 75. Systematic E C, jaworski J C, kaiser J D, et al. Systematic review of topical cosmetic derivatives [ J ]. 3262. Biological inhibitors [ B.1.: 3262. Biological salts of topical cosmetic inhibitors [ J ]. 62. Biological inhibitors of topical cosmetic inhibitors [ J ]. 1. Biological inhibitors of topical cosmetic inhibitors of topical drugs, and topical drugs of topical drugs [ 1. Biological inhibitors of topical drugs, 3262. In the present application of topical cosmetic inhibitors [ B.. TCI, while not causing atrophy of the skin, is locally irritating and causes itching, and some patients are intolerant (Piotr, ciechanowicz, adriana, et al jak-inhibitors of current opinion and future applications [ J ] Journal of Dermatological Treatment, 2018). The topical JAK inhibitors can inhibit skin inflammation and improve skin barrier function, but may cause skin infections such as folliculitis, acne, kaposi's spot rash and herpes simplex (2 am, tabassum s. Cyclosporine in dermatology.

In cases where topical anti-inflammatory drugs are not palliative, systemic therapeutic drugs should be used in combination, including the use of oral antihistamines, immunosuppressants, oral TCS, and biologies. Oral antihistamines can relieve itching in patients, but are less effective and therefore are only adjunctive therapy; immunosuppressive agents such as cyclosporin inhibit interleukins and relieve itching, but they are nephrotoxic and induce hypertension and hyperlipidemia, and thus they are not used for more than 1 year (Yu, sherry, H, et al. A systematic review of the safety and efficacy of systematic microbiological agents in atomic disorders [ J]Journal of the American Academy of Dermatology, 2018.); oral TCS can be used for a short time for acute exacerbations or severe lesions, but it is systemicGreat adverse reactions (

M,Srour J,Walter A,Knop M,Wollenberg A.Dupilumab for treatment of atopic dermatitis[J].Expert Rev Clin Pharmacol.2018,11(5):467-474.)。

Eczema is often treated in a non-persistent manner due to the long course of eczema, easy recurrence, inconvenient treatment, side effects, lack of trust in doctors and the like (Yang Z, liu M, wang W, et al. Pseudolaric acid B injuries and patient pain dermatitis-like skin lesions by inhibiting the invasion of interstitial-17-induced inflammation [ J ] Rep,2017,7 (1): 7918.). Therefore, the medicine which is effective, has low side effect and is convenient to use is selected, the compliance of patients can be greatly improved, the curative effect is improved, the existing medicines are difficult to simultaneously meet the three points and can be used for a long time, and the traditional Chinese medicine treatment has unique advantages in this aspect. AD belongs to the categories of eczema, blood-wind sore, acute eczema, scrofula and the like in traditional Chinese medicine. Modern Chinese medicine summarizes the pathogenesis of the disease as body deficiency, the pathogenic wind-damp heat as the target, and the disease is considered to be caused by the intrinsic deficiency and the exogenous pathogenic wind-damp heat, which cause the accumulation of wind-damp heat in the body and the invasion of skin.

The external medicine formula principle for clinically treating eczema is mainly to expel wind, dampness and heat evil, is assisted by killing parasites to relieve itching, and is used for detoxifying and promoting granulation of skin with scratching and ulceration.

Disclosure of Invention

The first purpose of the invention is to provide a traditional Chinese medicine composition for treating eczema.

The second purpose of the invention is to provide the application of the traditional Chinese medicine composition for treating eczema in preparing the cream for relieving itching and promoting healing.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

the invention provides a traditional Chinese medicine composition for treating eczema, which is prepared from the following components in parts by weight: 5 to 15 parts of golden larch bark, 5 to 15 parts of wild chrysanthemum flower, 5 to 15 parts of garden burnet root, 5 to 15 parts of wrinkled gianthyssop herb, 5 to 15 parts of oriental wormwood, 4 to 10 parts of fineleaf schizonepeta herb, 3 to 8 parts of divaricate saposhnikovia root, 3 to 8 parts of common cnidium fruit, 3 to 8 parts of mint, 1 to 5 parts of amur corktree bark, 1 to 5 parts of myrrh and 3 to 8 parts of liquorice.

The traditional Chinese medicine composition for treating eczema is prepared from the following components in parts by weight: 10 parts of golden larch bark, 10 parts of wild chrysanthemum flower, 10 parts of carbonized sanguisorba root, 10 parts of wrinkled gianthyssop herb, 10 parts of oriental wormwood, 7 parts of fineleaf schizonepeta herb, 5 parts of divaricate saposhnikovia root, 5 parts of common cnidium fruit, 5 parts of mint, 3 parts of golden cypress, 3 parts of myrrh and 5 parts of liquorice.

The traditional Chinese medicine composition for treating eczema is prepared from the following components in parts by weight: 15 parts of golden larch bark, 5 parts of wild chrysanthemum flower, 15 parts of carbonized sanguisorba root, 15 parts of wrinkled gianthyssop herb, 15 parts of oriental wormwood, 10 parts of fineleaf schizonepeta herb, 8 parts of divaricate saposhnikovia root, 8 parts of common cnidium fruit, 8 parts of mint, 5 parts of amur corktree bark, 5 parts of myrrh and 8 parts of liquorice.

The traditional Chinese medicine composition for treating eczema is prepared from the following components in parts by weight: 5 parts of golden larch bark, 15 parts of wild chrysanthemum flower, 5 parts of carbonized sanguisorba root, 5 parts of wrinkled gianthyssop herb, 5 parts of oriental wormwood, 4 parts of fineleaf schizonepeta herb, 3 parts of divaricate saposhnikovia root, 3 parts of common cnidium fruit, 3 parts of mint, 2 parts of amur corktree bark, 2 parts of myrrh and 3 parts of liquorice.

In the traditional Chinese medicine composition for treating eczema, the monarch drug is golden larch bark, and the ministerial drugs are fructus cnidii, wild chrysanthemum, wrinkled gianthyssop and mint; the adjuvant materials include herba Artemisiae Scopariae, herba Schizonepetae, radix Saposhnikoviae, charred radix Sangusorbae, cortex Phellodendri, and Myrrha; the guiding drug is licorice.

The nature, taste, meridian tropism and function of each medicine in the formula and the common dosage are as follows:

golden larch bark: nature and taste: pungent and warm; is toxic. Meridian tropism: it enters lung and spleen meridians. Function: kill parasites and relieve itching. The dosage is as follows: proper dosage is for external application.

Wild chrysanthemum: nature and taste: bitter, pungent and slightly cold. Meridian tropism: it enters liver and heart meridians. Function: clearing away heat and toxic material. The dosage is as follows: proper dosage is for external application.

Charring the garden burnet root: nature and taste: bitter, sour, astringent and slightly cold. Meridian tropism: it enters liver and large intestine meridians. The functions are as follows: cool blood and stop bleeding, remove toxicity and heal wound. The dosage is as follows: proper dosage is for external application.

Agastache rugosus: property and taste: pungent and warm. Meridian tropism: it enters lung, spleen and stomach meridians. The functions are as follows: regulate the middle warmer, remove dirt and dampness. The dosage is as follows: proper dosage is for external application.

Herba artemisiae scopariae: property and taste: bitter, pungent and slightly cold. Meridian tropism: it is also found in the spleen, stomach, liver and gallbladder meridians. The functions are as follows: clear damp-heat and alleviate jaundice. The dosage is as follows: proper dosage is for external application.

Herba schizonepetae: nature and taste: pungent and warm. Meridian tropism: it enters lung and liver meridians. The functions are as follows: relieve exterior syndrome, dispel wind and promote eruption. The dosage is as follows: proper dosage is for external application.

Wind prevention: nature and taste: pungent, sweet and warm. Meridian tropism: it enters bladder, liver and spleen meridians. The functions are as follows: relieving exterior syndrome, dispelling pathogenic wind, and eliminating dampness. The dosage is as follows: proper dosage is for external application.

Fructus cnidii: property and taste: pungent, bitter and warm; has little toxicity. Meridian tropism: it enters kidney meridian. The functions are as follows: dry dampness, dispel wind, kill parasites. The dosage is as follows: proper dosage is for external application.

Mint: nature and taste: pungent and cool. Meridian tropism: it enters lung and liver meridians. The functions are as follows: disperse wind-heat. The dosage is as follows: proper dosage is for external application.

Phellodendron bark: nature and taste: bitter and cold. It enters kidney and bladder meridians. Function: clear heat and dry dampness, remove toxicity and cure sore. The dosage is as follows: proper dosage is for external application.

Myrrh: nature and taste: bitter and neutral. Meridian tropism: enter liver meridian. The functions are as follows: disperse blood and remove stasis, relieve swelling and alleviate pain. The dosage is as follows: proper dosage is for external application.

Licorice root: nature and taste: sweet and neutral. Meridian tropism: it enters heart, lung, spleen and stomach meridians. Function: clear heat and remove toxicity, and harmonize the recipe. The dosage is as follows: proper dosage is for external application.

In the formula, the golden larch bark and the common cnidium fruit are used for dispelling wind, killing parasites and relieving itching, the wild chrysanthemum flower and the garden burnet root are used for clearing heat and removing toxicity, the oriental wormwood, the golden cypress and the wrinkled gianthyssop herb are used for clearing heat, drying dampness and killing parasites, the fineleaf schizonepeta herb, the divaricate saposhnikovia root and the mint are used for clearing heat, relieving exterior syndrome and dispelling wind, the myrrh is used for detumescence and promoting granulation, and the liquorice is used for relieving the drug toxicity. The medicines are mixed to play the effects of clearing heat, dispelling wind, removing dampness, killing parasites and relieving itching.

The second aspect of the invention provides application of the traditional Chinese medicine composition for treating eczema in preparation of an itching-relieving and healing-promoting cream.

The preparation method of the itching-relieving and healing-promoting cream comprises the following steps:

firstly, mixing golden larch bark, wild chrysanthemum flower, carbonized sanguisorba root, wrinkled gianthyssop herb, oriental wormwood, fineleaf schizonepeta herb, divaricate saposhnikovia root, common cnidium fruit, mint, amur corktree bark, myrrh and liquoric root according to a ratio, adding 8-12 (preferably 12) times of distilled water, decocting for 1-3 (preferably 2) times, 1-2 (preferably 2) hours each time, and combining water extracts for the next step; obtaining the volatile oil of the medicinal materials;

step two, filtering the water extract obtained in the previous step to be clear, adding 80% ethanol until the alcohol concentration in the water extract reaches a preset value, standing overnight at 4 ℃, filtering, and concentrating under reduced pressure distillation until no alcohol smell exists to obtain an alcohol precipitation water extract;

thirdly, the formula of the itching-relieving and healing-promoting cream is as follows: oil phase: 15 to 22g of stearic acid, 6 to 10g of glycerin monostearate, 25 to 35g of liquid paraffin, 8 to 12g of vaseline and 0.5 to 1g of the medicinal material volatile oil prepared in the first step; water phase: 4-8 g of triethanolamine, 25-35 g of glycerol and 180-210 g of alcohol precipitation water extract prepared in the second step;

according to the dosage of each component, the oil phase and the water phase are respectively mixed, the mixture is heated to be liquefied under the condition that the temperature is 75-85 ℃ (preferably 80 ℃), the two phases are mixed while the mixture is hot, the mixture is stirred for 10-15min, and the mixture is cooled to obtain the cream for relieving itching and promoting healing.

Preferably, the formula of the itching-relieving and healing-promoting cream comprises the following components: oil phase: 18g of stearic acid, 8.1g of glyceryl monostearate, 32.1g of liquid paraffin, 9.9g of vaseline and 0.75g of the medicinal material volatile oil prepared in the first step; water phase: 6g of triethanolamine, 30g of glycerol and 8978 g of the alcohol precipitation water extract prepared in the second step.

Due to the adoption of the technical scheme, the invention has the following advantages and beneficial effects:

in the traditional Chinese medicine composition, the golden larch bark and the common cnidium fruit are used for dispelling wind, killing parasites and relieving itching, the wild chrysanthemum flower and the garden burnet root are used for clearing heat and removing toxicity, the oriental wormwood, the golden cypress and the wrinkled gianthyssop are used for clearing heat, drying dampness and killing parasites, the schizonepeta, the divaricate saposhnikovia root and the mint are used for clearing heat, relieving exterior syndrome and dispelling wind, the myrrh is used for reducing swelling and promoting granulation, and the liquorice is used for relieving the toxicity of the medicine. The medicines are mixed to play the efficacies of clearing heat, dispelling wind, eliminating dampness, killing parasites and relieving itching.

The itching-relieving and healing-promoting cream preparation is suitable for treating acute, subacute and chronic eczema, has good curative effect, obviously improves erythema, desquamation, lichenification and pruritus of an eczema mouse, can reduce the thickness of the skin of the mouse, reduces the serum IgE level of the mouse and has no obvious adverse reaction.

Drawings

FIG. 1 is a schematic representation of skin lesion expression in each group of mice.

FIG. 2 is a schematic representation of pathological sections of mice in each group.

Detailed Description

In order to more clearly illustrate the invention, the invention is further described below in connection with preferred embodiments. It is to be understood by persons skilled in the art that the following detailed description is illustrative and not restrictive, and is not to be taken as limiting the scope of the invention.

Example 1

A traditional Chinese medicine composition for treating eczema is prepared from the following components in parts by weight: 10g of golden larch bark, 10g of wild chrysanthemum flower, 10g of carbonized sanguisorba root, 10g of wrinkled gianthyssop herb, 10g of oriental wormwood, 7g of fineleaf schizonepeta herb, 5g of divaricate saposhnikovia root, 5g of common cnidium fruit, 5g of mint, 3g of amur corktree bark, 3g of myrrh and 5g of liquorice.

The preparation method of the Chinese medicinal composition decoction comprises the following steps:

decocting in water for 2 times, adding 960mL of water for the first time, and decocting for 180min to obtain a first decoction; adding water to 960mL for the second time (including residue of the first decoction), and decocting for 180min to obtain a second decoction; combining the first and second decoctions to obtain a water decoction.

Example 2

A traditional Chinese medicine composition for treating eczema is prepared from the following components in parts by weight: 15g of golden larch bark, 5g of wild chrysanthemum flower, 15g of carbonized sanguisorba root, 15g of wrinkled gianthyssop herb, 15g of oriental wormwood, 10g of fineleaf schizonepeta herb, 8g of divaricate saposhnikovia root, 8g of common cnidium fruit, 8g of mint, 5g of amur corktree bark, 5g of myrrh and 8g of liquorice.

Example 3

A traditional Chinese medicine composition for treating eczema is prepared from the following components in parts by weight: 5g of golden larch bark, 15g of wild chrysanthemum flower, 5g of carbonized sanguisorba root, 5g of wrinkled gianthyssop herb, 5g of oriental wormwood, 4g of fineleaf schizonepeta herb, 3g of divaricate saposhnikovia root, 3g of common cnidium fruit, 3g of mint, 2g of amur corktree bark, 2g of myrrh and 3g of liquorice.

Example 4

1. The preparation method of the itching-relieving and healing-promoting cream comprises the following steps:

weighing 10g of golden larch bark, 10g of wild chrysanthemum flower, 10g of carbonized sanguisorba root, 10g of wrinkled gianthyssop herb, 10g of oriental wormwood, 7g of fineleaf schizonepeta herb, 5g of divaricate saposhnikovia root, 5g of common cnidium fruit, 5g of mint, 3g of amur corktree bark, 3g of myrrh and 5g of liquorice, and totally 80g. Three for designing water adding quantity, decocting time and decocting timesFactor, three-level orthogonal test, plotting L ₉ (3 ⁴ ) Orthogonal table (as shown in table 1), optimizes the extraction process of the cream for relieving itching and promoting healing, and finally obtains the extract with 12 times of distilled water, decocting for 2 times, the extract with the highest score after 3 hours every time, according to the extract yield (extract yield = dry powder (g)/total amount of medicinal materials (g) × 100%) obtained by drying water extract in an evaporation dish after concentrating and the content comprehensive score of three effective components of chlorogenic acid, golden larch bark acetic acid and osthole, which is shown in table 2.

TABLE 1 orthogonal test factor horizon

TABLE 2 extraction of antipruritic and healing promoting extract L ₉ (3 ⁴ ) Orthogonal table design and results

The optimal method comprises the following steps: mixing cortex pseudolaricis 10g, flos Chrysanthemi Indici 10g, charred radix Sangusorbae 10g, herba Agastaches 10g, herba Artemisiae Scopariae 10g, herba Schizonepetae 7g, radix Saposhnikoviae 5g, fructus Cnidii 5g, herba Menthae 5g, cortex Phellodendri 3g, myrrha 3g, and Glycyrrhrizae radix 5g, and mixing to total 80g. Adding 12 times of distilled water, decocting for 2 times, each time for 2 hours, and mixing the water extracts to obtain 1200mL for the next step; and obtaining 0.6ml of medicinal material volatile oil.

2. Optimization of alcohol precipitation process of itching-relieving and healing-promoting cream

Filtering 1200mL of the water extract obtained in the last step until the water extract is clear, performing an alcohol precipitation test, and setting the alcohol precipitation concentration as a study factor, wherein the three levels are respectively set to be 60%, 70% and 80%. Adding ethanol until the ethanol concentration in the water extract reaches a preset value, transferring to a refrigerator, and standing at 4 deg.C overnight. Filtering the water extract after alcohol precipitation, observing that 60 percent and 70 percent alcohol precipitation are carried out, and observing that the precipitate is less than 80 percent, considering that the cream for relieving itching and promoting healing needs transdermal absorption to play a role, carrying out alcohol precipitation by 80 percent concentration. Concentrating the ethanol precipitation solution by vacuum distillation until no alcohol smell is observed, and measuring the densityIs 1.006g/cm ³ . The extract is used as water phase, and is stored in a refrigerator at 4 ℃ for later use.

The optimal method comprises the following steps: filtering 1200mL of the water extract obtained in the last step to be clear, adding 80% ethanol until the alcohol concentration in the water extract reaches a preset value, standing overnight at 4 ℃, filtering, distilling and concentrating under reduced pressure until no alcohol smell exists, obtaining 300g of alcohol precipitation water extract which is used as a water phase, and storing at 4 ℃ for the next step.

3. Forming process of itching-relieving and healing-promoting cream

Formula 1: oil phase: 18g of stearic acid, 8.1g of glycerin monostearate, 32.1g of liquid paraffin, 9.9g of vaseline and 0.75g of the medicinal material volatile oil prepared in the first step; water phase: 6g of triethanolamine, 30g of glycerol and 8978 g of the alcohol precipitation water extract prepared in the second step;

and (2) formula: oil phase: 18g of stearic acid, 6g of glyceryl monostearate, 36g of liquid paraffin, 12g of vaseline and 0.2g of the medicinal material volatile oil prepared in the first step; water phase: 26.4g of glycerol and 195g of the alcohol precipitation water extract prepared in the second step;

and (3) formula: oil phase: 15g of stearic acid, 12g of glyceryl monostearate, 30g of liquid paraffin, 12g of vaseline and 0.2g of the medicinal material volatile oil prepared in the first step; water phase: 3g of span 80, 12g of Tween 80, 30g of glycerol and 185.4g of the alcohol precipitation water extract prepared in the second step;

and (4) formula: oil phase: 10g of beeswax, 10g of paraffin, 50g of liquid paraffin, 10g of vaseline and 0.75g of the medicinal material volatile oil prepared in the first step; water phase: 24g of glyceryl monostearate, 4g of span 80, 2g of Tween 80 and 100g of the ethanol precipitation water extract prepared in the second step;

and (5) formula: oil phase: 16g of paraffin, 64g of liquid paraffin, 8g of vaseline and 0.75g of the medicinal material volatile oil prepared in the first step; water phase: 16g of glyceryl monostearate, 1.6g of span 80 and 100g of alcohol precipitation water extract prepared in the second step;

and (6) formula: oil phase: 12g of beeswax, 20g of vaseline, 68g of liquid paraffin and 0.75g of the medicinal material volatile oil prepared in the first step; water phase: 8g of lanolin, 16g of glyceryl monostearate, 0.8g of Tween 80 and 100g of the ethanol precipitation water extract prepared in the second step;

and (3) formula 7: oil phase: 15g of stearic acid, 30g of hexadecanol, 15g of vaseline and 0.75g of the medicinal material volatile oil prepared in the first step; water phase: 6g of glyceryl monostearate, 9g of sodium dodecyl sulfate and 186g of the ethanol precipitation water extracting solution prepared in the second step;

and (4) formula 8: oil phase: 3g of stearic acid, 24g of hexadecanol, 30g of vaseline and 0.75g of the medicinal material volatile oil prepared in the first step; water phase: 12g of glyceryl monostearate, 6g of sodium dodecyl sulfate and 186g of the ethanol precipitation water extracting solution prepared in the second step;

and (3) formula 9: oil phase: 30g of stearic acid, 6g of lanolin, 18g of hexadecanol, 15g of vaseline and 0.75g of the medicinal material volatile oil prepared in the first step; water phase: 3g of glyceryl monostearate, 3g of sodium dodecyl sulfate and 186g of the ethanol precipitation water extracting solution prepared in the second step;

the formula 10 is as follows: oil phase: 24g of stearic acid, 6g of lanolin, 15g of hexadecanol, 6g of vaseline and 0.75g of the medicinal material volatile oil prepared in the first step; water phase: 21g of glyceryl monostearate, 3g of sodium dodecyl sulfate and 186g of the ethanol precipitation water extracting solution prepared in the second step;

the preparation method of the itching-relieving and healing-promoting cream comprises the following steps: mixing the oil phase and the water phase according to the above components, heating in 80 deg.C water bath to liquefy, mixing the two phases while hot, stirring with electric stirrer at 400rpm for 10-15min, and cooling to obtain the final product. And (5) cooling in the shade, and storing in a dry place for later use.

3.1 appearance of the cream

Selecting 10W/O matrix formulas, grading the cream matrix according to the ductility, granular feel and color of the cream, and finally selecting the formula with the highest score.

The results for the 10 formulated creams are as follows:

formula 1: the paste is moderate in thickness, white in color, fine, soft and easy to spread.

And (2) formula: the paste is thick and thin, white, yi Tu spread and has rough feel.

And (3) formula: the paste is thick, thin and white, yi Tuzhan and has a slightly granular feel.

And (4) formula 4: the paste is thick, white, not easy to spread and rough.

And (5) formula: the paste is thick and white, and Yi Tu is spread and is not easy to clean.

And (6) formula: the paste is thick and thin, white in color, yi Tuzhan, and slightly rough in spreading.

And (3) formula 7: the paste was thick, white in color Yi Tuzhan.

And (4) formula 8: the paste is thick and white, yi Tuzhan, and is difficult to clean after being spread.

Formula 9: the paste is moderate in thickness, milky white, easy to spread and not easy to clean.

Formula 10: the paste is thick and white, and the paste is slightly caked and difficult to spread.

3.2 Cold and Heat resistance test

Heat resistance test: respectively taking 5g of No. 1-10 paste, respectively filling the paste into a sealed plastic box, putting the sealed plastic box into a constant-temperature oven at 55 ℃, and observing that oil-water stratification occurs in formulas 2, 3 and 6 after 6 hours, oil-water stratification slightly occurs in formulas 7 and 8, and oil-water stratification does not occur in other formulas.

Cold resistance test: respectively taking 5g of No. 1-10 paste, respectively filling the paste into sealed plastic boxes, and placing the plastic boxes into a refrigerator at the temperature of-15 ℃ for 24 hours to observe. The cream matrix is put into a refrigerator with the temperature of-15 ℃, has no special change, and is found through comprehensive investigation: formulation 1 cream base formulation is preferred.

Example 5

Pharmacodynamic study of itching-relieving and healing-promoting cream on eczema mice

The 32 mice are randomly divided into 4 groups, each group comprises 8 mice, and the groups respectively comprise a blank group (A), a model group (B), an itching relieving and healing promoting cream group (C) and a cream matrix group (prepared by removing volatile oil from an oil phase and removing an alcohol precipitation water extract from a water phase in the formula 1) (D). Four groups of mice had their back hair removed one day before the experiment, and the eczema model was established: the mice d1 to d4 in the three groups except the blank group were subjected to skin sensitization by applying 1% DNBC-acetone olive oil solution 200. Mu.l to the back (acetone: olive oil = 3:1), the application was suspended at d5 to d8, and it was considered that the model was successfully made when the skin of the back of the mice was dry and desquamated on day 8, and the skin was maintained by applying DNBC-acetone olive oil solution 200. Mu.l daily on day 9 until the experiment was completed on day 22. And (3) drug treatment: the itching-relieving and healing-promoting cream group and the cream base group were applied with 1g of the corresponding cream 4 hours after application of 0.5% DNBC-acetone olive oil solution, starting on day 9, continuously for 14 days until day 22, and the mice were sacrificed on day 23.

1. Skin damage and scratching frequency of mice

As shown in fig. 1, fig. 1 is a schematic representation of skin lesion expression in each group of mice. A is blank group, B is model group, C is itching-relieving and healing-promoting cream group, and D is cream base group. The figure shows that the erythema, desquamation, scratch and incrustation of the model group are more serious than those of the blank group, and the erythema, desquamation, scratch and incrustation of the itching-relieving and healing-promoting cream group are improved than those of the model group; the skin damage score and scratching frequency of the model group mice are both larger than those of the blank group (p is less than 0.01); compared with the model group, the itch-relieving and heal-promoting cream group and the cream matrix group have reduced skin damage scores and scratching times (p is less than 0.05); compared with the cream matrix group, the skin damage score of the itching relieving and healing promoting cream group is reduced (p is less than 0.05), and the details are shown in a table 2.

TABLE 2 comparison of skin lesions and scratching times in mice: (

n＝8)

Note: in comparison to the blank set, the data is, ^▲ p is less than 0.05; in comparison to the set of models, ^* P＜0.05

2. pathological section of mouse skin (HE staining)

As shown in fig. 2, fig. 2 is a schematic diagram of pathological sections of mice in each group. A is blank group, B is model group, C is itching-relieving and healing-promoting cream group, and D is cream base group. When the mouse skin is observed under an HE staining microscope, compared with a blank group, the model group has the defects of disordered structure of each layer of skin, hyperkeratosis of epidermis, obvious hypertrophy of a spinous layer, more inflammatory cell infiltration and disappearance of hair follicle structure; compared with a model group, the itching-relieving and healing-promoting cream group has the advantages that the structure of each layer of skin is clear, the hypertrophy of an epidermis acantho layer is reduced, a small amount of dermal blood vessels are dilated, inflammatory cells are obviously reduced, and the hair follicle structure is obviously increased; the thickness of the epidermis of the mouse in the model group is larger than that of the blank group (p < 0.01); compared with the model group, the itch-relieving and heal-promoting cream group and the cream matrix group have reduced epidermis thickness (p is less than 0.05); compared with the cream matrix group, the thickness of the epidermis of the itching relieving and healing promoting cream group is reduced (p is less than 0.05).

3. Mouse IgE levels

Serum IgE levels of the model group mice are larger than those of a blank group (p < 0.01); compared with the model group, the serum IgE level of the itching-relieving and healing-promoting cream group and the cream matrix group is reduced (p is less than 0.05), and the details are shown in a table 3.

TABLE 3 comparison of epidermal thickness and serum IgE levels in mice: (

n＝8)

Although the present invention has been described with reference to a preferred embodiment, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims

1. The traditional Chinese medicine composition for treating eczema is characterized by being prepared from the following components in parts by weight: 5 to 15 parts of golden larch bark, 5 to 15 parts of wild chrysanthemum flower, 5 to 15 parts of garden burnet root charcoal, 5 to 15 parts of wrinkled gianthyssop herb, 5 to 15 parts of oriental wormwood, 4 to 10 parts of fineleaf schizonepeta herb, 3 to 8 parts of divaricate saposhnikovia root, 3 to 8 parts of common cnidium fruit, 3 to 8 parts of mint, 1 to 5 parts of amur corktree bark, 1 to 5 parts of myrrh and 3 to 8 parts of liquorice.

2. The traditional Chinese medicine composition for treating eczema as claimed in claim 1, wherein the traditional Chinese medicine composition for treating eczema is prepared from the following components in parts by weight: 10 parts of golden larch bark, 10 parts of wild chrysanthemum flower, 10 parts of carbonized sanguisorba root, 10 parts of wrinkled gianthyssop herb, 10 parts of oriental wormwood, 7 parts of fineleaf schizonepeta herb, 5 parts of divaricate saposhnikovia root, 5 parts of common cnidium fruit, 5 parts of mint, 3 parts of amur corktree bark, 3 parts of myrrh and 5 parts of liquorice.

3. The traditional Chinese medicine composition for treating eczema as claimed in claim 1, wherein the traditional Chinese medicine composition for treating eczema is prepared from the following components in parts by weight: 15 parts of golden larch bark, 5 parts of wild chrysanthemum flower, 15 parts of carbonized sanguisorba root, 15 parts of wrinkled gianthyssop herb, 15 parts of oriental wormwood, 10 parts of fineleaf schizonepeta herb, 8 parts of divaricate saposhnikovia root, 8 parts of common cnidium fruit, 8 parts of mint, 5 parts of amur corktree bark, 5 parts of myrrh and 8 parts of liquorice.

4. The traditional Chinese medicine composition for treating eczema as claimed in claim 1, wherein the traditional Chinese medicine composition for treating eczema is prepared from the following components in parts by weight: 5 parts of golden larch bark, 15 parts of wild chrysanthemum flower, 5 parts of carbonized sanguisorba root, 5 parts of wrinkled gianthyssop herb, 5 parts of oriental wormwood, 4 parts of fineleaf schizonepeta herb, 3 parts of divaricate saposhnikovia root, 3 parts of common cnidium fruit, 3 parts of mint, 2 parts of amur corktree bark, 2 parts of myrrh and 3 parts of liquorice.

5. Use of the traditional Chinese medicine composition for treating eczema as claimed in any one of claims 1 to 4 in preparation of an itching-relieving and healing-promoting cream.

6. The use according to claim 5, wherein the preparation method of the itching-relieving and healing-promoting cream comprises the following steps:

firstly, mixing golden larch bark, wild chrysanthemum flower, carbonized sanguisorba root, wrinkled gianthyssop herb, oriental wormwood, fineleaf schizonepeta herb, divaricate saposhnikovia root, common cnidium fruit, mint, amur corktree bark, myrrh and liquoric root according to a ratio, adding 8-12 times of distilled water, decocting for 1-3 times, and 1-2 hours each time, and combining water extracts for the next step; obtaining the volatile oil of the medicinal materials;

step two, filtering the water extract obtained in the step one until the water extract is clear, adding 80% ethanol until the alcohol concentration in the water extract reaches a preset value, standing overnight at 4 ℃, filtering, and concentrating by reduced pressure distillation until no alcohol smell exists to obtain an alcohol precipitation water extract;

according to the dosage of each component, the oil phase and the water phase are respectively mixed, the mixture is heated to be liquefied under the condition that the temperature is 75-85 ℃, the two phases are mixed while the mixture is hot, stirred for 10-15min and cooled, and the itching-relieving and healing-promoting cream is obtained.

7. The use according to claim 6, wherein the antipruritic healing promoting cream formulation is: oil phase: 18g of stearic acid, 8.1g of glyceryl monostearate, 32.1g of liquid paraffin, 9.9g of vaseline and 0.75g of the medicinal material volatile oil prepared in the first step; water phase: 6g of triethanolamine, 30g of glycerol and 8978 g of the alcohol precipitation water extract prepared in the second step.