CN115344174A - Micropore plate sample adding guiding method and system - Google Patents

Abstract

The invention discloses a microplate sample adding guiding method and a system, comprising the following steps: step 1, a user clicks through a display module to start creating a pipetting plan and selects the type of a used microplate; step 2, the display module displays the patterns corresponding to the hole sites of the microplate according to the type of the microplate, and enters a pipetting plan making interface, and a user makes a pipetting plan through the pipetting plan making module; step 3, when a user needs to actually sample the microporous plate, the display module guides and prompts the actual sample adding of the user according to a pipetting plan, and the display module displays the actual sample adding state in real time; and 4, after the user finishes the pipetting plan in the actual operation, the processing module generates an operation report for the user to refer to. The invention can assist the user to sample the microporous plate, save the operation record and reduce the error rate and the workload of the user.

Description

Micropore plate sample adding guiding method and system

Technical Field

The invention relates to the technical field of life science experiment assistance, in particular to a microplate sample adding guiding method and a microplate sample adding guiding system.

Background

In a traditional laboratory, before pipetting 96-or 384-well plates, researchers need to determine the type, volume and loading sequence of samples loaded in each well by writing data and drawing a table, and when loading, the samples need to be added step by step against the hand-drawn table. Because the micropore plate has numerous holes and is densely distributed, researchers are easy to make errors of adding wrong holes and adding wrong samples. Meanwhile, in the experimental process, very strict requirements are imposed on the use records of equipment and consumables. This is a necessary and cumbersome process for the researcher, requiring a great deal of effort.

In current technical scheme, micropore board application of sample device passes through board groove embedding micropore board, uses the pilot lamp that corresponds with micropore board hole site position to assist the researcher to carry out the application of sample, can effectively reduce application of sample error. However, this type of device cannot adjust the number of indicator lights and cannot adapt to microplates having different numbers of wells. When only a few wells are available in the microplate, the self-contained sequencing functionality of the device is inflexible and inefficient. Meanwhile, the researchers still need to manually draw the form on the notebook to carry out contrast sample adding, the problem of the sample adding error which possibly occurs is not solved, and the researchers need to automatically complete the sample adding report in the working process, so that the workload in the sample adding process is large, and the error of the workers in the process is easily caused.

Disclosure of Invention

The invention aims to provide a microplate sample adding guiding method, which is used for solving the problems that the existing sample adding auxiliary method is high in error rate and cannot record a sample adding process.

In one aspect, an embodiment of the present invention provides a microplate sample application guiding method, including the following steps:

step 1, a user clicks through a display module to start creating a pipetting plan and selects the type of a used microplate;

step 2, the display module displays patterns of hole positions of the corresponding micro-porous plate according to the type of the micro-porous plate, and enters a pipetting plan making interface, and a user makes a pipetting plan through the pipetting plan making module;

step 3, when a user needs to carry out actual sample adding on the microporous plate, the display module guides and prompts the actual sample adding of the user according to the pipetting plan, and the display module displays the actual sample adding state in real time;

and 4, after the user finishes the pipetting plan in the actual operation, the processing module generates an operation report for the user to refer to.

Further, in the step 1, the types of the micro-porous plates include a 96-well plate and a 384-well plate, and the hole sites of the micro-porous plates of the same type are all fixed positions.

Further, the step 2 further comprises:

step 2-1, a user creates a reagent card according to requirements, selects the reagent card and fills corresponding hole sites in the schematic diagram of the microporous plate, and then reagent filling is completed;

2-2, creating content cards according to requirements by a user, forming a content card list to be added, selecting the content cards, and filling corresponding hole sites in the schematic diagram of the microporous plate to finish content filling;

step 2-3, after the reagent and the content are filled, selecting the type of the used pipettor and constructing a pipettor card;

and 2-4, after the model of the pipettor is determined, formulating the pipetting sequence of the contents to be added in each hole site to complete the formulation of the pipetting plan.

Further, in the step 2-1 and the step 2-2, the name and volume information of the substance to be added are included in the reagent card and the content card, and the color of the reagent card is customized by the user.

Further, in the step 2-2, a filling mode is selected according to the number of channels of the liquid transfer device, the number of channels of the liquid transfer device comprises a single channel, eight channels, twelve channels, sixteen channels and twenty-four channels, and the filling mode comprises single-hole filling, single-column filling, full-row filling and dragging of multiple holes in any rectangular range;

when the number of channels is a single channel, a single-hole, single-column, whole-row and dragging arbitrary rectangular range multiple-hole filling mode is supported;

when the number of the channels is eight channels or sixteen channels, the whole column filling is supported;

when the number of channels is twelve and twenty-four, full row fill is supported.

Further, in step 2-3, constructing a pipette card further includes:

if the pipettor that the in-service use was waited to add possesses the bluetooth function, then open the bluetooth button after, the software can be with the automatic pipettor information input that supports the bluetooth connection for the pipettor card, if the pipettor does not have the bluetooth function, then through the newly-built pipettor card of user, input pipettor relevant information.

Further, in the step 2-4, the step of formulating a pipetting sequence further comprises: and setting the content adding sequence in each hole site on the basis of the sequence of firstly filling the reagent and then filling the content according to the content required to be added in each hole site by the user.

Further, the step 3 further includes: and (3) making the content and the volume of the sample to be added and the hole site position to be added into each time into a sample adding card by the guidance software according to a pipetting plan formulated by the user in the step (2), and displaying the sample adding card on the left side of the display module in a list manner, wherein the sample adding card is sequenced according to a pipetting sequence formulated by the user and corresponds to the light spot at the corresponding hole site position in the diagram of the microplate on the right side.

Further, in the step 4, the operation report further includes basic information of the pipetting operation, a pipetting plan and an operation log;

wherein, the basic information comprises a user, time and a used liquid transfer device; the pipetting plan comprises the type and volume of the sample added to each well in each pipetting step set by the user; the operation log comprises time and content information of each step of operation when the user actually samples.

On the other hand, the embodiment of the invention also provides a microplate sample addition guiding system, which comprises: the device comprises a pipetting plan making module, a pipetting guide module, a Bluetooth connecting module and a display module;

the pipetting plan making module can make a user make a pipetting plan, including selection of a type of the microplate, reagents and contents to be added, pipettes used, and a specific adding mode and sequence;

the Bluetooth connecting module transmits information between the pipettor and the display module through a Bluetooth technology to realize interaction;

the liquid transferring guide module comprises a sample adding guide card list displayed on the left side of the display module, a microplate schematic diagram on the right side and a microplate fixing device;

the display module is the display screen, can realize user interaction, and the user can realize the formulation of liquid-transfering plan, observe liquid-transfering guide process, look over and derive liquid-transfering report through the display module.

The invention has the beneficial effects that: the microplate sample application guiding method provided by the invention can help researchers to use software to formulate a liquid transfer plan before the start of an experiment, and plan the filling content, the liquid transfer sequence and the type of a liquid transfer gun of each step of liquid transfer, so that the problem of complexity in formulating the liquid transfer plan is solved, and the liquid transfer efficiency is greatly improved;

the method supports users to import or export the pipetting plan, can realize cooperation of different users, solves the problem that team sharing cannot be carried out, and can improve the efficiency of the team experiment process;

the invention can prompt the position of next pipetting and the content to be added of a user by using the sample adding content card and the light spot guide mode, assists researchers to finish pipetting, reduces the pipetting error probability and greatly improves the pipetting efficiency;

after the user finishes the pipetting plan, the invention can automatically record the whole experiment flow and operation log, provide data support for subsequent recording and analysis and solve the problems of data recording and storage.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts. In the drawings:

fig. 1 is a schematic flow chart of a microplate sample application guiding method provided in an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a microplate sample-adding guidance system provided in an embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the embodiments of the present invention are further described in detail below with reference to the accompanying drawings. The exemplary embodiments and descriptions of the present invention are provided to explain the present invention, but not to limit the present invention.

In the description of the present specification, the terms "comprising," "including," "having," "containing," and the like are used in an open-ended fashion, i.e., to mean including, but not limited to. Reference to the description of the terms "one embodiment," "a particular embodiment," "some embodiments," "for example," etc., means that a particular feature, structure, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the application. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. The sequence of steps involved in the embodiments is for illustrative purposes to illustrate the implementation of the present application, and the sequence of steps is not limited and can be adjusted as needed.

The embodiment of the invention provides a microplate sample adding guiding method, fig. 1 is an overall flow schematic diagram of the method, and as shown in fig. 1, the method comprises the following steps:

step 1, a user clicks through a display module to start creating a pipetting plan and selects the type of a used microplate;

the types of the micro-porous plates comprise 96-pore plates and 384-pore plates, and the pore positions of the micro-porous plates of the same type are all fixed positions.

Step 2, the display module displays the patterns corresponding to the hole sites of the microplate according to the type of the microplate, and enters a pipetting plan making interface, and a user makes a pipetting plan through the pipetting plan making module;

and the display module displays the patterns of the corresponding hole sites of the micro-porous plate for subsequent reference of the user according to the type of the micro-porous plate selected by the user.

The contents filled in each hole site of the microplate in the pipetting plan comprise reagent filling and content filling, and because only one hole can be filled with one sample in each reagent or content filling, a content filling link can add a plurality of content filling steps in order to fill a plurality of substances in one hole.

For example, 10ul of water is added during the filling of the reagent, and in the filling of the contents, in order to add contents to one well site several times, a first filling of the contents may be performed by adding 1ul of saline to the well site, and a second filling of the contents may be performed by adding 1ul of sugar water to the well site.

The pipetting plan includes the reagent or content names, volumes and locations of addition that are required to be added, as well as the type of pipetter used and the fill sequence of the ensemble. And in each pipetting step, the user fills in the name and volume of the reagent or content to be added through the pipetting plan making module.

Specifically, the user formulates the pipetting plan through the pipetting plan formulating module further comprises:

2-1, creating a reagent card according to the requirement by a user, selecting the reagent card, and filling corresponding hole sites in the schematic diagram of the micropore plate to finish reagent filling;

and 2-2, creating content cards according to requirements by a user, forming a content card list to be added, selecting the content cards, and filling corresponding holes in the schematic diagram of the microporous plate to finish content filling.

The reagent card and the content card comprise the name and volume information of the substance to be added, and a user selects the reagent card and the content card and then determines the adding position in the microporous plate; the reagent card color supports the HSV color selector to select custom colors, the user can set the colors of the reagent card in the colors corresponding to the reagent, and when the user needs to add the reagent, the reagent card can present the colors corresponding to the reagent.

Preferably, the reagent card is presented in the microplate illustration in a color format and the content card is presented in the microplate illustration in a textual format. Wherein, the color form is presented in the diagram of the micro-porous plate, which means that after a certain reagent is selectively added to a certain hole position, the hole position is changed into the color corresponding to the reagent card. Different reagent cards distinguish through name, volume and colour, and when adding the card, the system can give a card colour, and the user also can oneself set up the colour.

Further, if a plurality of substances are required to be added into one hole, a user needs to add a plurality of content filling steps, add a content card in each content filling step, and fill the hole after determining the content card.

The reagent cards and the content cards only appear in the corresponding pipetting steps, e.g., a certain reagent card only appears in the reagent filling step and not in the content filling step.

The card list to be added is displayed on the display module, and comprises reagent cards or content cards added by the user in the current pipetting step, and the cards in the card list can be randomly sequenced, modified or deleted by the user.

When a reagent is added, the reagent card will also present the corresponding color of the reagent. The card only appears during the corresponding pipetting step, e.g. reagent a card only appears during the reagent filling step and not during the content filling step.

And selecting a filling mode according to the channel number of the pipettor, wherein the filling mode further comprises single-hole, single-column, whole-row and dragging multi-hole filling in any rectangular range.

Further, reagent addition is carried out according to the number of channels of the liquid transfer device in a filling mode, wherein the number of channels of the liquid transfer device comprises a single channel, eight channels, twelve channels, sixteen channels and twenty-four channels;

when the number of channels is a single channel, a single-hole, single-column, whole-row and dragging arbitrary rectangular range multi-hole filling mode is supported, when the number of channels is eight channels or sixteen channels, only whole-column filling is supported, and when the number of channels is twelve channels and twenty-four channels, only whole-row filling is supported.

Step 2-3, after the reagent and the content are filled, selecting the type of the used pipettor and constructing a pipettor card;

wherein, the addition mode of pipettor model includes: selecting the model of the used pipettor through Bluetooth connection or custom setting;

constructing a pipette card further comprises:

if the pipettor that the in-service use was waited to add possesses the bluetooth function, then open the bluetooth button after, the software can be with the automatic pipettor information input that supports the bluetooth connection for the pipettor card, if the pipettor does not have the bluetooth function, then through the newly-built pipettor card of user, input pipettor relevant information.

The constructed pipettor card is stored in a region to be selected on the display interface, and a user can select the pipettor by dragging the pipettor card to a specific position through dragging interaction, so that the pipettor card required to be used for the pipetting is selected.

Step 2-4, after the model of the pipettor is determined, formulating the pipetting sequence of the contents to be added at each hole site to complete formulation of a pipetting plan;

in step 2-1 and step 2-2, the user only determines the contents to be added in each well site, and does not determine the sample addition sequence between the well sites. Step 2-1 and step 2-2 include reagent filling and content filling, where the content filling may be multiple times.

The step 2-4 of determining the pipetting sequence is that a user determines the adding sequence of each content according to the content required to be added to each hole site, so that the user is guided by the sequence of adding the samples to the hole sites in the actual operation.

The pipetting sequence comprises: according to the content required to be added in each hole site, on the basis of the sequence of filling the reagent and then filling the content, the user sets the content adding sequence in each hole site as the pipetting sequence.

Preferably, in this embodiment, the liquid types are prioritized by default, the liquid contents of the same type are pipetted according to the pipetting route from left to right and from top to bottom, and the user can customize the pipetting route.

The liquid type priority ordering means that the same liquid is loaded in sequence, and after the loading of one liquid is completed, another liquid is refilled.

Furthermore, after a pipetting plan is formulated, software reminds a user to install the microplate fixing device through a demonstration video and a picture, a microplate is placed at a correct position, and a schematic diagram with the same physical size as an actual microplate can be displayed on the display module.

Step 3, when a user needs to carry out actual sample adding on the microporous plate, the display module guides and prompts the actual sample adding of the user according to the pipetting plan, and the display module displays the actual sample adding state in real time;

and (3) manufacturing the content and volume of each sample addition and the position to be added into the sample by the guidance software according to the pipetting plan formulated by the user in the step (2) into a sample adding card, and displaying the sample adding card on the left side of the display module in a list manner. The cards are ordered according to the pipetting sequence set by the user, and correspond to the light points at the corresponding positions in the diagram of the microplate on the right side.

The information contained in the sample adding card is the name and volume of the substance and the position of the hole site on the microporous plate, the position can be represented by numbering the hole site, for example, the rows are numbered from A to Z according to the alphabetical order, the hole site of each row is numbered according to the Arabic number order, and the sample adding card is used for being matched with the light spot of the hole site to prompt a user about the content of the reagent added at the current hole site. The card only appears in the process of guiding pipetting, and the highlight display of the light spot informs the user of which hole site the pipetting is currently carried out, and what the contents need to be added.

The sample adding is manually completed on the micro-porous plate by a user through actual operation, and the actual operation of the user is guided by the content displayed on the display module. In the application of sample in-process, the current hole and the corresponding card of application of sample can highlight among the display module, and the guide user moves the liquid. When the light spot moves, a gradual change ribbon moves along with the light spot, the gradual change ribbon is in a cross shape, the transparency of the ribbon is gradually changed linearly from two ends to the middle, the central point is the hole to be added with the sample, the gradual change ribbon can enable a user to clearly see which current hole position needs to be added with the sample, and the position of the light spot in the screen needs to be calculated in real time according to the column number and the row number in the whole process.

Further, the guidance process includes the following two modes according to whether the actually used pipettor has the function of being connected with the computer:

if the pipettor does not have the function of being connected with the computer, then guide and move the liquid in-process, there is a graphic representation unanimous with micropore board physical dimension on the display module, and the user can pass through the subassembly with the micropore board fix the back on the screen, by the user according to guide application of sample by oneself.

In order to match the guiding with the action of the user, after each sample adding, the user needs to click a next button on the screen or enter the guiding of the next step in a pedal mode after connecting a Bluetooth pedal.

If the pipettor has the function of being connected with the computer, for example, when the pipettor actually used can pass through the electronic pipettor connected with the bluetooth, the data can be synchronized to the pipettor by the established pipetting plan, and the pipettor is operated, so that the user only needs to move the position of the pipettor according to the steps.

Further, after completing one step in the pipetting process, the user can proceed to the next step in three ways: clicking a next button; using a Bluetooth pedal; a Bluetooth-connected pipettor automatically senses;

during pipetting, the user can perform the previous step, the next step, the pause and the pause at any time.

And 4, after the user finishes the pipetting plan in the actual operation, the processing module generates an operation report for the user to refer to.

Specifically, after all pipetting steps are completed, the user can choose to stay in the current interface or view pipetting results.

The operation report also comprises basic information of pipetting operation, a pipetting plan and an operation log;

wherein, the basic information comprises a user, time and a used liquid transfer device; the pipetting plan comprises the type and volume of the sample added to each well in each pipetting step set by the user; the operation log comprises time and content information of each step of operation when the user actually samples.

Further, the user can directly export the operation report through the display module.

The invention also provides a microplate sample addition guiding system, the microplate sample addition guiding method provided above can be realized by depending on the system, the system in the invention can be developed by An Zhuoduan, programmed by java language and transplanted into a tablet personal computer, and the software system interactive carrier is the tablet personal computer.

The microplate sample adding guiding system further comprises a liquid transfer plan making module, a liquid transfer guiding module, a Bluetooth connecting module and a display module;

the pipetting plan making module is used for creating a card containing information such as name and volume according to the content of the reagent to be added by a user. After the user selects the card, the filling is performed by touch in the schematic diagram of the micro-porous plate. After the software acquires the card information corresponding to each hole site, according to the sequence of reagent filling and content filling, the hole site information determined in each filling step is made into a sample adding guide card according to the pipetting sequence selected by a user.

The Bluetooth connecting module transmits information between the pipettor and the display device through the Bluetooth technology to realize interaction. After the Bluetooth connection is completed, the pipettor sends information including a pipettor serial number, a brand, a range and the like to the display device, and the display module receives the information and generates a pipettor card. Subsequently, the display module transmits the prepared pipetting plan information to the pipettor through the Bluetooth function. The pipettor is programmed according to the volume of each sample addition in the pipetting plan. In the pipetting process, the user uses the pipettor to complete one-time sample adding according to the guidance of the display device and the pipettor, and then the pipettor transmits the information to the display module. The user selects the pipetting process in the display module to enter the next step.

The liquid-transfering guide module comprises a sample-adding guide card list displayed on the left side of the display module, a microplate schematic diagram on the right side and a microplate fixing device. The content and the sequence of the sample adding guide card are generated by the established liquid transfer plan, and the user is prompted to obtain the card content corresponding to the current sample adding step through highlighting. The schematic diagram of the micro-porous plate on the right side enables the size and the position of the hole site in the schematic diagram to strictly correspond to the real micro-porous plate through a method of converting physical dimensions by px. After the microplate fixing device is installed and the microplate is placed, when a certain hole site needs sample adding, in the schematic diagram of the microplate, the hole site displays a highlight light spot. At the user's visual angle, can see that certain hole site bottom appears the highlight in the micropore board, the suggestion user adds reagent content according to left side card content.

The display module can be a liquid crystal screen, the function of the display module comprises the realization of user interaction, and a user can realize the formulation of a pipetting plan, the observation of a pipetting guide process, the viewing and the derivation of a pipetting report through the display module.

As will be appreciated by one skilled in the art, embodiments of the present invention may be provided as a method, system, or computer program product. Accordingly, the present invention may take the form of an entirely hardware embodiment, an entirely software embodiment or an embodiment combining software and hardware aspects. Furthermore, the present invention may take the form of a computer program product embodied on one or more computer-usable storage media (including, but not limited to, disk storage, CD-ROM, optical storage, and the like) having computer-usable program code embodied therein.

The present invention is described with reference to flowchart illustrations and/or block diagrams of methods, apparatus (systems), and computer program products according to embodiments of the invention. It will be understood that each flow and/or block of the flow diagrams and/or block diagrams, and combinations of flows and/or blocks in the flow diagrams and/or block diagrams, can be implemented by computer program instructions. These computer program instructions may be provided to a processor of a general purpose computer, special purpose computer, embedded processor, or other programmable data processing apparatus to produce a machine, such that the instructions, which execute via the processor of the computer or other programmable data processing apparatus, create means for implementing the functions specified in the flowchart flow or flows and/or block diagram block or blocks.

These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including instruction means which implement the function specified in the flowchart flow or flows and/or block diagram block or blocks.

These computer program instructions may also be loaded onto a computer or other programmable data processing apparatus to cause a series of operational steps to be performed on the computer or other programmable apparatus to produce a computer implemented process such that the instructions which execute on the computer or other programmable apparatus provide steps for implementing the functions specified in the flowchart flow or flows and/or block diagram block or blocks.

The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are only exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims (10)

1. A microplate sample application guiding method is characterized by comprising the following steps:

step 1, a user clicks through a display module to start creating a pipetting plan and selects the type of a used microplate;

step 2, the display module displays the patterns corresponding to the hole sites of the microplate according to the type of the microplate, and enters a pipetting plan making interface, and a user makes a pipetting plan through the pipetting plan making module;

step 3, when a user needs to carry out actual sample adding on the microporous plate, the display module guides and prompts the actual sample adding of the user according to the pipetting plan, and the display module displays the actual sample adding state in real time;

and 4, after the user finishes the pipetting plan in the actual operation, the processing module generates an operation report for the user to refer to.

2. The microplate sample application guiding method of claim 1, wherein in step 1, microplate types comprise 96-well plates and 384-well plates, and the wells of the same type of microplate are all fixed positions.

3. The microplate sample application guiding method of claim 1, wherein said step 2 further comprises:

step 2-1, a user creates a reagent card according to requirements, selects the reagent card and fills corresponding hole sites in the schematic diagram of the microporous plate, and then reagent filling is completed;

2-2, creating content cards according to requirements by a user, forming a content card list to be added, selecting the content cards, and filling corresponding hole sites in the schematic diagram of the micropore plate to complete content filling;

2-3, after the reagent and the content are filled, selecting the type of the used pipettor and constructing a pipettor card;

and 2-4, after the model of the pipettor is determined, formulating the pipetting sequence of the contents to be added at each hole site to complete the formulation of the pipetting plan.

4. The microplate sample application guiding method of claim 3, wherein in step 2-1 and step 2-2, the name and volume information of the substance to be added is included in the reagent card and the content card, and the color of the reagent card is customized by the user.

5. The microplate sample application guiding method according to claim 3 or 4, wherein in step 2-2, the filling manner is selected according to the number of channels of the pipettes, the number of channels of the pipettes includes single channel, eight channels, twelve channels, sixteen channels and twenty-four channels, and the filling manner includes single-hole, single-column, whole-row, and dragging of multiple holes in any rectangular range;

when the number of channels is a single channel, a single-hole, single-column, whole-row and dragging arbitrary rectangular range multiple-hole filling mode is supported;

when the number of the channels is eight channels or sixteen channels, the whole column filling is supported;

when the number of channels is twelve and twenty-four, full row fill is supported.

6. The microplate sampling guidance method of claim 3, wherein in steps 2-3, constructing a pipette card further comprises:

if the pipettor that the in-service use was waited to add possesses the bluetooth function, then open the bluetooth button after, the software can be with the automatic pipettor information input that supports the bluetooth connection for the pipettor card, if the pipettor does not have the bluetooth function, then through the newly-built pipettor card of user, input pipettor relevant information.

7. The microplate sample application guidance method of claim 3, wherein in steps 2-4, the formulating a pipetting sequence further comprises: and setting the content adding sequence in each hole site on the basis of the sequence of firstly filling the reagent and then filling the content according to the content required to be added in each hole site by the user.

8. The microplate sample application guiding method of claim 1, wherein step 3 further comprises: and (3) making the content and the volume of the sample to be added and the hole site position to be added into each time into a sample adding card by the guidance software according to a pipetting plan formulated by the user in the step (2), and displaying the sample adding card on the left side of the display module in a list manner, wherein the sample adding card is sequenced according to a pipetting sequence formulated by the user and corresponds to the light spot at the corresponding hole site position in the diagram of the microplate on the right side.

9. The microplate sample application guidance method of claim 1, wherein in step 4, the operation report further includes basic information of pipetting operations, pipetting plans, and operation logs;

wherein, the basic information comprises a user, time and a used liquid transfer device; the pipetting plan comprises the type and volume of the sample added to each well in each pipetting step set by the user; the operation log comprises time and content information of each step of operation when the user actually samples.

10. A microplate sample application guidance system, comprising: the device comprises a pipetting plan making module, a pipetting guide module, a Bluetooth connecting module and a display module;

the pipetting plan making module can make a user make a pipetting plan, including selection of a type of the microplate, reagents and contents to be added, pipettes used, and a specific adding mode and sequence;

the Bluetooth connecting module transmits information between the pipettor and the display module through a Bluetooth technology to realize interaction;

the pipetting guide module comprises a sample adding guide card list displayed on the left side of the display module, a microplate schematic diagram on the right side and a microplate fixing device;

the display module is the display screen, can realize user interaction, and the user can realize the formulation of liquid-transfering plan, observe liquid-transfering guide process, look over and derive liquid-transfering report through the display module.

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