CN115327745B - High-flux flat-field apochromatic objective lens - Google Patents
High-flux flat-field apochromatic objective lens Download PDFInfo
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- CN115327745B CN115327745B CN202211005330.0A CN202211005330A CN115327745B CN 115327745 B CN115327745 B CN 115327745B CN 202211005330 A CN202211005330 A CN 202211005330A CN 115327745 B CN115327745 B CN 115327745B
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- 230000005499 meniscus Effects 0.000 claims abstract description 37
- 238000012163 sequencing technique Methods 0.000 abstract description 15
- 238000003384 imaging method Methods 0.000 abstract description 12
- 230000004907 flux Effects 0.000 abstract description 9
- 230000000007 visual effect Effects 0.000 abstract description 5
- 230000004075 alteration Effects 0.000 description 17
- 206010010071 Coma Diseases 0.000 description 4
- 201000009310 astigmatism Diseases 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000006059 cover glass Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B13/00—Optical objectives specially designed for the purposes specified below
- G02B13/001—Miniaturised objectives for electronic devices, e.g. portable telephones, webcams, PDAs, small digital cameras
- G02B13/0055—Miniaturised objectives for electronic devices, e.g. portable telephones, webcams, PDAs, small digital cameras employing a special optical element
- G02B13/006—Miniaturised objectives for electronic devices, e.g. portable telephones, webcams, PDAs, small digital cameras employing a special optical element at least one element being a compound optical element, e.g. cemented elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
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- Wood Science & Technology (AREA)
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Lenses (AREA)
Abstract
The invention discloses a high-flux flat-field apochromatic objective lens, which comprises a first lens group, a second lens group and a third lens group which are coaxially arranged in sequence from an object space to an image space, wherein the first lens group comprises a first meniscus lens and a second meniscus lens; the second lens group comprises a first bonding lens consisting of a first biconvex lens, a first biconcave lens and a second biconvex lens, a third biconvex lens with positive focal power and a second bonding lens consisting of a third meniscus lens and a fourth biconvex lens; the third lens group includes a third cemented lens composed of a fourth meniscus lens and a fifth meniscus lens, and a fourth cemented lens composed of a second biconcave lens and a fifth biconvex lens. The embodiment of the invention can simultaneously increase the imaging visual field and the numerical aperture, improve the sequencing flux and can be widely applied to the technical field of optics.
Description
Technical Field
The invention relates to the technical field of optics, in particular to a high-flux flat-field apochromatic objective lens.
Background
The sequencing flux is one of the core indexes of the gene sequencer, and represents the data output in unit time; the sequencing flux affects the output time of the detection result and also relates to the sequencing cost. In the sequencer, the core optics that affect the sequencing flux is the microscope objective. There are two core parameters in the microscope objective: one is the numerical aperture, which determines the imaging resolution of the objective, the size of which determines the density of DNA on the sequencing chip; the other is an imaging visual field, the larger the visual field is, the larger the range of one shooting is, and the shorter the time for completing scanning imaging of the sequencing chip is. It follows that high numerical aperture and large field objective are critical to improving sequencing throughput.
Imaging field and numerical aperture are two key parameters that determine sequencing flux, but these two parameters are often balanced, i.e., the field of view of a large numerical aperture objective is smaller, while the numerical aperture of the objective is smaller, which cannot simultaneously compromise both large imaging field and high definition.
Disclosure of Invention
In view of the above, an object of the embodiments of the present invention is to provide a high-flux flat-field apochromatic objective lens, which can realize a large imaging field of view and a large numerical aperture at the same time, and improve sequencing flux.
The embodiment of the invention provides a high-flux flat-field apochromatic objective lens, which comprises a first lens group, a second lens group and a third lens group which are coaxially arranged in sequence from an object side to an image side,
the first lens group comprises a first meniscus lens and a second meniscus lens which are arranged in sequence;
the second lens group comprises a first biconvex lens, a first biconcave lens, a second biconvex lens, a third meniscus lens and a fourth biconvex lens which are sequentially arranged, wherein the first biconvex lens, the first biconcave lens and the second biconvex lens form a first bonding lens, and the third meniscus lens and the fourth biconvex lens form a second bonding lens;
the third lens group comprises a fourth meniscus lens, a fifth meniscus lens, a second biconcave lens and a fifth biconvex lens which are sequentially arranged, wherein the fourth meniscus lens and the fifth meniscus lens form a third cemented lens, and the second biconcave lens and the fifth biconvex lens form a fourth cemented lens.
Optionally, the focal length of the first lens group satisfies the following relation:
10.2<f L23 /f<11
wherein f L23 And f represents the focal length of the objective lens.
Optionally, the focal length of the first cemented lens satisfies the following relation:
6.42<f L456 /f<7.15
wherein f L456 And f represents the focal length of the objective lens.
Optionally, the focal length of the third biconvex lens satisfies the following relation:
2.91<f L7 /f<3.32
wherein f L7 Represents the focal length of the third biconvex lens, f represents the objective lensIs provided for the focal length of (a).
Optionally, the focal length of the second cemented lens satisfies the following relation:
10.6<f L89 /f<12.3
wherein f L89 And f represents the focal length of the objective lens.
Optionally, the focal length of the third cemented lens satisfies the following relation:
-6.01<f L1011 /f<-5.66
wherein f L1011 And f represents the focal length of the objective lens.
Optionally, the focal length of the fourth cemented lens satisfies the following relation:
-69.1<f L1213 /f<-70.2
wherein f L1213 And f represents the focal length of the objective lens.
Optionally, all lenses of the first lens group, the second lens group, and the third lens group are spherical lenses.
The embodiment of the invention has the following beneficial effects: in this embodiment, the objective lens includes three lens groups coaxially arranged from an object side to an image side, and the first lens group includes two meniscus lenses; the second lens group comprises two cemented lenses and a biconvex lens with medium optical power, wherein a first cemented lens consisting of two biconvex lenses and a biconcave lens, and a second cemented lens consisting of a meniscus lens and a biconvex lens; the third lens group includes two cemented lenses, wherein the third cemented lens is composed of two meniscus lenses, and the fourth cemented lens is composed of one biconcave and one biconvex; light emitted by an object to be detected firstly passes through the first lens group to reduce the aperture angle, then one or more of spherical aberration, coma aberration or chromatic aberration is corrected through the second lens group, and then one or more of field curvature, astigmatism or chromatic aberration is eliminated through the third lens group, so that the imaging visual field and numerical aperture are increased simultaneously, and the sequencing flux is improved.
Drawings
FIG. 1 is a schematic structural diagram of a high-flux flat field apochromatic objective lens provided by an embodiment of the present invention;
FIG. 2 is an MTF curve of a high-flux flat field apochromatic objective lens provided by an embodiment of the present invention;
FIG. 3 is a point column diagram of a high flux flat field apochromatic objective lens provided by an embodiment of the present invention;
FIG. 4 is a field curvature and distortion curve of a high-flux flat-field apochromatic objective lens provided by an embodiment of the present invention;
fig. 5 is a diffraction energy distribution curve of a high-flux flat-field apochromatic objective lens provided by an embodiment of the present invention.
Detailed Description
The invention will now be described in further detail with reference to the drawings and to specific examples. The step numbers in the following embodiments are set for convenience of illustration only, and the order between the steps is not limited in any way, and the execution order of the steps in the embodiments may be adaptively adjusted according to the understanding of those skilled in the art.
As shown in fig. 1, an embodiment of the present invention provides a high-flux flat field apochromatic objective lens, including a first lens group G1, a second lens group G2, and a third lens group G3 coaxially arranged in order from an object side to an image side, wherein,
the first lens group G1 includes a first meniscus lens L2 and a second meniscus lens L3 arranged in sequence;
the second lens group G2 includes a first biconvex lens L4, a first biconcave lens L5, a second biconvex lens L6, a third biconvex lens L7, a third meniscus lens L8, and a fourth biconvex lens L9, which are sequentially arranged, wherein the first biconvex lens L4, the first biconcave lens L5, and the second biconvex lens L6 combine with a first cemented lens, and the third meniscus lens L8 and the fourth biconvex lens L9 form a second cemented lens;
the third lens group G3 includes a fourth meniscus lens L10, a fifth meniscus lens L11, a second biconcave lens L12, and a fifth biconvex lens L13, which are sequentially disposed, the fourth meniscus lens L10 and the fifth meniscus lens L11 constitute a third cemented lens, and the second biconcave lens L12 and the fifth biconvex lens L13 constitute a fourth cemented lens.
Specifically, L1 is a cover glass of a sample, and can also be glass of the upper layer of a flow channel of a sequencing chip. The first lens group G1 comprises L2 and L3, forms a front-arranged bright surface, collects light signals with large divergence angles and converts the light signals into light signals with small angles, and avoids generating excessive spherical aberration and/or coma aberration while effectively increasing the numerical aperture. The second lens group G2 includes lenses L4 to L9, and is used to correct spherical aberration, coma aberration, and chromatic aberration. The third lens group G3 includes lenses L10 to L13 for eliminating curvature of field, astigmatism and chromatic aberration, where L11 is a thick meniscus lens.
Optionally, the focal length of the first lens group satisfies the following relation:
10.2<f L23 /f<11
wherein f L23 And f represents the focal length of the objective lens.
In particular, referring to FIG. 1, f L23 The focal length of the first lens group formed by lenses L2 and L3 is indicated.
Optionally, the focal length of the first cemented lens satisfies the following relation:
6.42<f L456 /f<7.15
wherein f L456 And f represents the focal length of the objective lens.
In particular, referring to FIG. 1, f L456 The focal length of the first cemented lens formed by lenses L4, L5, and L6 is shown.
Optionally, the focal length of the third biconvex lens satisfies the following relation:
2.91<f L7 /f<3.32
wherein f L7 And f represents the focal length of the objective lens.
In particular, referring to FIG. 1, f L7 The focal length of the lens L7 is indicated.
Optionally, the focal length of the second cemented lens satisfies the following relation:
10.6<f L89 /f<12.3
wherein f L89 And f represents the focal length of the objective lens.
In particular, referring to FIG. 1, f L89 The focal length of the second cemented lens formed by lenses L8 and L9 is shown.
Optionally, the focal length of the third cemented lens satisfies the following relation:
-6.01<f L1011 /f<-5.66
wherein f L1011 And f represents the focal length of the objective lens.
In particular, referring to FIG. 1, f L1011 The focal length of the third cemented lens formed by lenses L10 and L11 is shown.
Optionally, the focal length of the fourth cemented lens satisfies the following relation:
-69.1<f L1213 /f<-70.2
wherein f L1213 And f represents the focal length of the objective lens.
In particular, referring to FIG. 1, f L1213 The focal length of the fourth cemented lens formed by lenses L12 and L13 is shown.
In a specific embodiment, the specific parameters of each lens in the objective lens are referred to in table one. Referring to FIG. 1, R1-R21 in FIG. 1 correspond to the face numbers 1-21 in Table one, respectively, in sequence.
List one
Referring to fig. 2, fig. 2 is a graph showing an MTF (Modulation Transfer Function ) curve of the objective lens in the above embodiment. As can be seen from fig. 2, the cut-off frequency of the objective lens is greater than 3100lp/mm, and the full field of view is close to the diffraction limit; the object lens has good image quality and well corrected aberration.
Referring to fig. 3, fig. 3 is a point chart of the objective lens in the above embodiment, and it can be seen from fig. 3 that the size of the full-field diffuse speckle is near the range of the airy speckle size at the visible wavelength. Referring to fig. 4, fig. 4 (a) is a field curve of the objective lens in the above embodiment, and fig. 4 (b) is a distortion curve of the objective lens in the above embodiment, as can be seen from fig. 4, the imaging field of view of the lens is very flat, and the distortion is less than 0.85%. As can be seen from the result data of fig. 3 and 4, the objective lens achieves the effect of apochromatic aberration in the flat field.
Referring to fig. 5, fig. 5 is a diffraction energy distribution curve of the objective lens in the above embodiment, where the abscissa is the diameter of the diffuse spot and the ordinate is the energy percentage of the diffuse spot. As can be seen from FIG. 5, 90% of the diffuse spot energy is concentrated in the range of 1um, and the energy concentration is high, so that the objective lens is particularly suitable for weak fluorescence signal imaging in gene sequencing.
In the specific embodiment, the numerical aperture of the objective lens is 0.75, the field diameter of an imaging object is 1.6mm, the apochromatic aberration of a flat field is reduced, and the focal length is 10mm, so that the parameters can meet most high-flux sequencing applications.
Optionally, all lenses of the first lens group, the second lens group, and the third lens group are spherical lenses.
Specifically, all lenses L2 to L13 in the first lens group to the third lens group are spherical lenses, thereby reducing the processing cost of the lenses.
The embodiment of the invention has the following beneficial effects: in this embodiment, the objective lens includes three lens groups coaxially arranged from an object side to an image side, and the first lens group includes two meniscus lenses; the second lens group comprises two cemented lenses and a biconvex lens with medium optical power, wherein a first cemented lens consisting of two biconvex lenses and a biconcave lens, and a second cemented lens consisting of a meniscus lens and a biconvex lens; the third lens group includes two cemented lenses, wherein the third cemented lens is composed of two meniscus lenses, and the fourth cemented lens is composed of one biconcave and one biconvex; light emitted by an object to be detected firstly passes through the first lens group to reduce the aperture angle, then one or more of spherical aberration, coma aberration or chromatic aberration is corrected through the second lens group, and then one or more of field curvature, astigmatism or chromatic aberration is eliminated through the third lens group, so that the imaging visual field and numerical aperture are increased simultaneously, and the sequencing flux is improved.
While the preferred embodiment of the present invention has been described in detail, the invention is not limited to the embodiment, and various equivalent modifications and substitutions can be made by those skilled in the art without departing from the spirit of the invention, and these modifications and substitutions are intended to be included in the scope of the present invention as defined in the appended claims.
Claims (2)
1. A high-flux flat field apochromatic objective lens is characterized by comprising a first lens group, a second lens group and a third lens group which are coaxially arranged in sequence from an object side to an image side,
the first lens group consists of a first meniscus lens and a second meniscus lens which are sequentially arranged;
the second lens group consists of a first biconvex lens, a first biconcave lens, a second biconvex lens, a third meniscus lens and a fourth biconvex lens which are sequentially arranged, wherein the first biconvex lens, the first biconcave lens and the second biconvex lens form a first bonding lens, and the third meniscus lens and the fourth biconvex lens form a second bonding lens;
the third lens group consists of a fourth meniscus lens, a fifth meniscus lens, a second biconcave lens and a fifth biconvex lens which are sequentially arranged, wherein the fourth meniscus lens and the fifth meniscus lens form a third cemented lens, and the second biconcave lens and the fifth biconvex lens form a fourth cemented lens;
the focal length of the first lens group satisfies the following relation:
10.2<f L23 /f<11
wherein f L23 Representing the focal length of the first lens group, f representing the focal length of the objective lens;
the focal length of the first cemented lens satisfies the following relation:
6.42<f L456 /f<7.15
wherein f L456 Representing the focal length of the first cemented lens;
the focal length of the third lenticular lens satisfies the following relationship:
2.91<f L7 /f<3.32
wherein f L7 Representing the focal length of the third lenticular lens;
the focal length of the second cemented lens satisfies the following relation:
10.6<f L89 /f<12.3
wherein f L89 Representing the focal length of the second cemented lens;
the focal length of the third cemented lens satisfies the following relation:
-6.01<f L1011 /f<-5.66
wherein f L1011 Representing the focal length of the third cemented lens;
the focal length of the fourth cemented lens satisfies the following relation:
-69.1<f L1213 /f<-70.2
wherein f L1213 Representing the focal length of the fourth cemented lens.
2. The objective lens of claim 1, wherein the first and second meniscus lenses are spherical lenses.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211005330.0A CN115327745B (en) | 2022-08-22 | 2022-08-22 | High-flux flat-field apochromatic objective lens |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211005330.0A CN115327745B (en) | 2022-08-22 | 2022-08-22 | High-flux flat-field apochromatic objective lens |
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| CN115327745A CN115327745A (en) | 2022-11-11 |
| CN115327745B true CN115327745B (en) | 2024-01-16 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108957718A (en) * | 2017-05-27 | 2018-12-07 | 南京理工大学 | A kind of wide spectrum flat field apochromatic micro objective |
| CN109061862A (en) * | 2018-08-27 | 2018-12-21 | 中国科学院苏州生物医学工程技术研究所 | A kind of micro objective of big field of view number |
| CN114326071A (en) * | 2021-12-14 | 2022-04-12 | 宁波永新光学股份有限公司 | 20-time large numerical aperture flat field apochromatic microscope objective |
| CN114815134A (en) * | 2021-01-20 | 2022-07-29 | 长春长光华大智造测序设备有限公司 | Achromatic microscope objective and optical system |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5445898B2 (en) * | 2007-11-02 | 2014-03-19 | 株式会社ニコン | Immersion microscope objective lens |
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- 2022-08-22 CN CN202211005330.0A patent/CN115327745B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108957718A (en) * | 2017-05-27 | 2018-12-07 | 南京理工大学 | A kind of wide spectrum flat field apochromatic micro objective |
| CN109061862A (en) * | 2018-08-27 | 2018-12-21 | 中国科学院苏州生物医学工程技术研究所 | A kind of micro objective of big field of view number |
| CN114815134A (en) * | 2021-01-20 | 2022-07-29 | 长春长光华大智造测序设备有限公司 | Achromatic microscope objective and optical system |
| CN114326071A (en) * | 2021-12-14 | 2022-04-12 | 宁波永新光学股份有限公司 | 20-time large numerical aperture flat field apochromatic microscope objective |
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