CN115312134A - Reagent development experiment method, device, terminal and storage medium - Google Patents
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Abstract
The invention discloses a reagent development experiment method, a device, a terminal and a storage medium, wherein the method comprises the following steps: determining a plurality of process combination results according to the type of the immunoreagent to be developed; sequencing the combined results of the plurality of processes to be used as an experiment sequence, setting the experiment sequence and carrying out experiments on the combined results of the processes according to the experiment sequence; and outputting the experimental results of the plurality of immunoreagents after the experiment is finished, and determining the target immunoreagent meeting the preset conditions from the plurality of immunoreagents according to the performance test result of each immunoreagent. The invention takes a plurality of predetermined flow combination results as an experiment sequence to automatically carry out the experiment, can quickly generate a plurality of immunoreagents, automatically detects the performance of each immunoreagent to determine the target immunoreagent meeting the preset conditions, not only can effectively improve the efficiency of reagent development, but also can effectively reduce the human error caused by human reagent development, thereby effectively improving the reliability of reagent development.
Description
Technical Field
The invention relates to the technical field of reagent development, in particular to a reagent development experiment method, a device, a terminal and a storage medium.
Background
The immunoassay technology is the detection technology with the most extensive application in the current biological detection method, has the characteristics of high accuracy, sensitivity and specificity, can be used for basic theory and application research of immunology, and can also be applied to various fields of biological research. Immunoassay techniques require the use of immunoreagents to achieve various types of immunoassays, where reagent development prior to use of immunoreagents is an important and critical process.
The existing reagent development experimental method is too dependent on manual completion of the reagent development process, so that the reagent development efficiency is low.
Disclosure of Invention
The invention provides a reagent development experimental method, a device, a terminal and a storage medium, which are used for solving the technical problem that the reagent development efficiency is low due to the fact that the existing reagent development experimental method excessively depends on manual completion of a reagent development process.
One embodiment of the present invention provides a reagent development experiment method, including:
determining a plurality of process combination results according to the type of the immunological reagent to be developed;
sequencing a plurality of flow combination results to serve as an experiment sequence, setting the experiment sequence and carrying out experiments on the flow combination results according to the experiment sequence;
and outputting the experimental results of a plurality of immunoreagents after the experiment is finished, and determining a target immunoreagent meeting preset conditions from the plurality of immunoreagents according to the performance test result of each immunoreagent.
Further, the determining a plurality of process combination results according to the type of the immunoreagent to be developed includes:
dividing the flow required by the immunological reagent to be developed according to the type of the immunological reagent to be developed to obtain a plurality of flow sets, wherein each flow set corresponds to one type of immunological reagent to be developed;
and carrying out permutation and combination processing on the flows in each flow set to obtain a plurality of flow combination results.
Further, the step of determining a target immunoreagent meeting a preset condition from a plurality of immunoreagents according to the performance test result of each immunoreagent comprises the following steps:
obtaining reaction values of a plurality of samples with known concentrations;
fitting the reaction values and parameter values of a plurality of the samples of known concentration to a fitted curve;
substituting the reaction value of each immunological reagent into the fitting curve to obtain the parameter value of the immunological reagent;
and when the parameter value of the immunological reagent meets a preset condition, judging that the immunological reagent meets the preset condition.
Further, when the parameter value of the immunoreagent meets a preset condition, determining that the immunoreagent meets the preset condition includes:
and judging whether the deviation value of the parameter value of the immunological reagent and a preset standard parameter value exceeds a preset threshold range, if not, judging that the parameter value of the immunological reagent meets a preset condition, and judging that the immunological reagent meets the preset condition.
Further, the setting of the experiment sequence and the experiment of the combined result of each process according to the experiment sequence includes:
judging whether a reagent for detecting the reaction value of the sample with the known concentration is stored in a detection instrument or not according to the position information corresponding to the reagent information, if so, calling the reagent, and detecting by adopting the reagent and configured process parameters to obtain the reaction values of the samples with the known concentration; if not, generating a reminding message to remind the user to reconfigure the reagent information.
Further, the fitting the response values and the parameter values of the plurality of samples with known concentrations to a fitting curve includes:
combining the reaction value and the parameter value of each known concentration sample into a data set;
and fitting a plurality of data sets into a fitting curve by adopting a preset fitting algorithm.
Further, the combining the response value and the parameter value of each of the samples with known concentration into a data set includes:
transforming the parameter value and the response value of each known concentration sample into logarithms to form a data set; or
And setting the value digits of decimal points for the parameter value and the reaction value of each known concentration sample to form a data set.
Further, before substituting the response value of each immunoreagent into the fitted curve to obtain the parameter value of the immunoreagent, the method further includes:
setting sampling parameters according to the detection requirements of parameter value detection;
collecting a plurality of original reaction values of each immunological reagent in the detection process according to the sampling parameters;
drawing a plurality of original reaction values into a peak-shaped curve, and selecting a section of luminous curve from the peak-shaped curve;
and taking the average value of a plurality of original reaction values in the luminescence curve as the reaction value of each immunoreagent.
Further, before determining the combined result of the plurality of procedures according to the type of the immunoreagent to be developed, the method further comprises the following steps:
the method comprises the steps of obtaining position information of a plurality of reagents in a detection instrument, and matching the position information of the plurality of reagents with corresponding reagent information.
One embodiment of the present invention provides a reagent development experimental apparatus, including:
the flow combination determining module is used for determining a plurality of flow combination results according to the type of the immunological reagent to be developed;
the test module is used for sequencing a plurality of flow combination results to serve as an experiment sequence, setting the experiment sequence and carrying out experiments on the flow combination results according to the experiment sequence;
and the reagent determining module is used for outputting the experiment results of a plurality of immunoreagents after the experiment is finished, and determining the target immunoreagent meeting the preset conditions from the plurality of immunoreagents according to the performance test result of each immunoreagent.
Further, the flow combination determination module is configured to:
dividing the flow required by the immunological reagent to be developed according to the type of the immunological reagent to be developed to obtain a plurality of flow sets, wherein each flow set corresponds to one type of immunological reagent to be developed;
and carrying out permutation and combination processing on the processes in each process set to obtain a plurality of process combination results.
Further, the reagent determination module comprises:
the known concentration sample reaction value detection unit is used for acquiring reaction values of a plurality of known concentration samples;
a fitting unit for fitting the reaction values and parameter values of a plurality of samples of known concentration to a fitted curve;
a parameter value obtaining unit, configured to substitute the reaction value of each immunoreagent into the fitting curve to obtain a parameter value of the immunoreagent;
and the immune reagent judging unit is used for judging that the immune reagent meets the preset condition when the parameter value of the immune reagent meets the preset condition.
Further, the immunoreagent determination unit is specifically configured to:
and judging whether the deviation value of the parameter value of the immunological reagent and a preset standard parameter value exceeds a preset threshold range, if not, judging that the parameter value of the immunological reagent meets a preset condition, and judging that the immunological reagent meets the preset condition.
Further, the known concentration sample reaction value detection unit is used for:
judging whether a reagent for detecting the reaction value of the sample with the known concentration is stored in a detection instrument or not according to the position information corresponding to the reagent information, if so, calling the reagent, and detecting by adopting the reagent and configured process parameters to obtain the reaction values of the samples with the known concentration; if not, generating reminding information to remind the user to reconfigure the reagent information.
Further, the fitting unit is specifically configured to:
combining the reaction value and the parameter value of each known concentration sample into a data set;
and fitting a plurality of data sets into a fitting curve by adopting a preset fitting algorithm.
Further, the combining the response value and the parameter value of each of the samples with known concentration into a data set includes:
transforming the parameter value and the response value of each known concentration sample into logarithms to form a data set; or
And setting the value digits of decimal points for the parameter value and the reaction value of each known concentration sample to form a data set.
Further, the parameter value obtaining unit is configured to:
setting sampling parameters according to the detection requirements of parameter value detection;
collecting a plurality of original reaction values of each immunoreagent in the detection process according to the sampling parameters;
drawing a plurality of original reaction values into a peak-shaped curve, and selecting a section of luminous curve from the peak-shaped curve;
taking the average of several original reaction values in the luminescence curve as the reaction value of each immunoreagent.
Further, the experimental apparatus further includes an information matching module, configured to:
the method comprises the steps of obtaining position information of a plurality of reagents in a detection instrument, and matching the position information of the plurality of reagents with corresponding reagent information.
An embodiment of the present invention provides a terminal, including a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor executes the computer program to implement the reagent development experiment method as described above.
One embodiment of the present invention provides a computer-readable storage medium comprising a stored computer program; wherein the computer program, when executed, controls the apparatus in which the computer readable storage medium is located to perform a reagent development assay method as described above.
The method comprises the steps of determining a plurality of process combination results according to types required by immune reagents to be developed, sequencing the plurality of process combination results to serve as an experiment sequence, carrying out experiments on the process combination results according to the experiment sequence to obtain a plurality of immune reagents, and carrying out performance tests on the immune reagents to determine the target immune reagents with composite preset conditions. The embodiment of the invention automatically performs the experiment by taking the predetermined multiple process combination results as the experiment sequence, can quickly generate multiple immunoreagents corresponding to different process combination results, automatically detects the performance of each immunoreagent to determine the target immunoreagent meeting the preset conditions, not only can effectively improve the efficiency of reagent development, but also can effectively reduce the human error caused by human reagent development, thereby effectively improving the reliability of reagent development.
Drawings
FIG. 1 is a schematic flow chart of a reagent development assay provided in an embodiment of the present invention;
FIG. 2 is a schematic flow chart of step S3 of the experimental method for reagent development provided in the embodiment of the present invention;
FIG. 3 is a schematic structural diagram of a reagent development experimental apparatus provided in an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a reagent determination module provided in an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present application will be described clearly and completely with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only some embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
In the description of the present application, it is to be understood that the terms "first", "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless otherwise specified.
In the description of the present application, it should be noted that, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
The reagent development experimental method provided by the embodiment of the invention can be suitable for various immunodetections, can be realized by software and/or hardware, and can be configured in computer equipment.
Referring to fig. 1, an embodiment of the present invention provides a reagent development experiment method, including:
s1, determining a plurality of process combined results according to the type of an immunoreagent to be developed;
the immunoreagent is a reagent made of specific antigen, antibody or related biological substance, and can be divided into various immunoreagents such as agglutination reaction, precipitation reaction, complement fixation reaction and labeled immunoreaction according to different methodologies.
Optionally, all flow combination results may be determined according to an enumeration method, and all flow combination results may be determined as a plurality of flow combination results in the above; and a plurality of flow combination results can be determined in a self-defined mode. For example, the combined results of multiple procedures requiring experiments are determined according to actual reagent development requirements.
S2, sequencing the combined results of the plurality of processes to serve as an experiment sequence, setting the experiment sequence and carrying out experiments on the combined results of the processes according to the experiment sequence;
in the embodiment of the invention, based on the determined combination results of the plurality of processes, a certain sequencing mode is used as an experiment sequence, and the experiment is carried out according to the experiment sequence to obtain the experiment results of the plurality of immunoreagents.
Optionally, each process combination result includes a plurality of sub-processes, including adding a sample, adding a diluent, diluting and mixing, adding a reagent, and the like. The embodiment of the invention can set the final flow combination result according to the actual reagent development requirement so as to generate a plurality of immune reagents.
And S3, outputting the experiment results of the plurality of immunoreagents after the experiment is finished, and determining the target immunoreagent meeting the preset conditions from the plurality of immunoreagents according to the performance test result of each immunoreagent.
In the embodiment of the present invention, after obtaining the multiple immunoreagents, performance verification needs to be performed on each immunoreagent, so as to determine, according to a performance test result of each immunoreagent, a target immunoreagent meeting a preset condition from the multiple immunoreagents. The performance verification qualified standard meeting the preset condition is, for example, an international standard or a standard set under another standard, and the like, and may be determined according to an actual situation.
Furthermore, the flow combination result corresponding to the target immune reagent is stored in the IC card, so that the detection instrument can repeatedly read and execute in subsequent detection, and different reagent development requirements are met.
In one embodiment, the combined results of the plurality of procedures are determined according to the type of immunoreagent to be developed, including:
dividing the processes required by the immunological reagent to be developed according to the type of the immunological reagent to be developed to obtain a plurality of process sets, wherein each process set corresponds to one type of immunological reagent to be developed;
and carrying out permutation and combination processing on the flows in each flow set to obtain a plurality of flow combination results.
In the embodiment of the invention, after the target immunoreagent meeting the preset conditions is determined from the multiple immunoreagents, the flow combination result of the target immunoreagent is stored in the IC card in a mode of writing the IC card, so that a detection instrument can repeatedly read and execute in subsequent detection, and different reagent development requirements are met. In one embodiment, the method for determining a target immunoreagent meeting a preset condition from a plurality of immunoreagents according to the performance test result of each immunoreagent comprises the following steps of:
s31, obtaining reaction values of a plurality of samples with known concentrations;
in the embodiment of the invention, the flow combination result comprises flow parameters and reagent information required by an experiment, and the position of the reagent in the detection instrument can be accurately obtained according to the position information corresponding to the reagent information, so that the reagent can be quickly called, and the reaction values of a plurality of samples with known concentrations are detected by combining the reagent and preset configured flow parameters.
S32, fitting the reaction values and the parameter values of the multiple samples with known concentrations into a fitting curve;
according to the embodiment of the invention, the parameter values and the reaction values of the samples with known concentrations are used as a group of data sets, and a preset fitting algorithm is adopted to fit the data sets into a fitting curve according to the linear relation between the parameter values and the reaction values so as to facilitate the subsequent analysis of the parameter values of the immunological reagents, wherein the preset fitting algorithm comprises a logistic fitting algorithm, and the parameter values comprise concentrations.
S33, substituting the reaction values of the immunological reagents into a fitting curve to obtain parameter values of the immunological reagents;
the fitting curve represents the linear relation between the reaction value and the parameter value, and the reaction value of the immunological reagent is substituted into the fitting curve in the embodiment of the invention, so that the parameter value of the immunological reagent can be estimated.
And S34, judging that the immunological reagent meets the preset condition when the parameter value of the immunological reagent meets the preset condition.
In one embodiment, when the parameter value of the immunoreagent satisfies the predetermined condition, determining that the immunoreagent satisfies the predetermined condition includes:
judging whether the deviation value of the parameter value of the reagent to be immunized and the preset standard parameter value exceeds a preset threshold range, if not, judging that the parameter value of the immunizing reagent meets a preset condition, judging that the immunizing reagent is correct, and generating and storing a corresponding project file according to a corresponding flow combination result so as to enable the detection instrument to call the flow combination result.
In the embodiment of the invention, the preset standard parameter value is the standard parameter value of the immunological reagent, the data of the flow combination result and the sample with the known concentration are stored in the evaluation file and named, the user can judge the flow combination result by clicking the evaluation file with the corresponding name in the evaluation list, when the deviation value of the standard parameter value of the immunological reagent and the parameter value obtained by testing the immunological reagent is not more than the preset threshold range, the immunological reagent is judged to be correct, and the flow combination result corresponding to the immunological reagent is stored on the IC card, so that a detection instrument can read and execute the flow combination result, and the convenience for developing the reagent is improved.
Further, in the embodiment of the present invention, after the immunoreagent is determined to meet the preset condition, the flow combination result corresponding to the immunoreagent and the fitting curve are combined to generate a project file in a HEX format, and the project file is stored on the IC card so as to be repeatedly read by the detection apparatus.
It should be noted that, after the immunoreagent meets the preset condition, the embodiment of the present invention further determines the correctness of the flow combination result by determining whether the data can be correctly loaded, for example, receiving data information selected by the user, determining whether the selected data information of the user, including the test peak data and the curve of the concentration, can be loaded correctly on the display interface, determining that the flow combination result of the embodiment of the present invention is correct if the data information can be loaded correctly, and determining that the flow combination result of the embodiment of the present invention is problematic if the data information cannot be loaded correctly, at this time, the flow needs to be redesigned by adjusting the configuration parameters and whether the flow combination result is correct again.
Furthermore, the embodiment of the invention can also repeatedly carry out detection for multiple times to obtain the parameter values of multiple immunological reagents, and carry out performance detection according to the multiple immunological reagents to judge whether the immunological reagents meet the preset conditions, thereby further improving the accuracy and reliability of development of the immunological reagents. As a specific implementation manner of the embodiment of the present invention, after the parameter values of more than 4 immunoreagents are obtained through detection, whether the immunoreagents meet the preset conditions is determined.
In one embodiment, obtaining response values for a plurality of samples of known concentration comprises:
judging whether a reagent for detecting the reaction values of the samples with known concentrations is stored in the detection instrument or not according to the position information corresponding to the reagent information, if so, calling the reagent, and detecting by adopting the reagent and configured process parameters to obtain the reaction values of the samples with known concentrations; if not, generating reminding information to remind the user to reconfigure the reagent information.
In the embodiment of the invention, if the detection instrument does not store the configured reagent for detecting the reaction value of the sample with the known concentration, a reminding message is generated to remind a user of reconfiguring the reagent, and after the actual information reconfigured by the user is received, whether the configured reagent for detecting the reaction value with the known concentration exists in the instrument is judged again until the configured reagent can be detected in the detection instrument, so that the reaction values of a plurality of samples with the known concentration are detected and obtained by adopting the reagent and the configured process.
In one embodiment, fitting the response values and parameter values for a plurality of samples of known concentration to a fitted curve comprises:
forming a data set by the reaction value and the parameter value of each sample with known concentration;
and fitting the plurality of data sets into a fitting curve by adopting a preset fitting algorithm.
In the embodiment of the invention, the preset fitting algorithm comprises a logistic fitting algorithm, a least square method and the like, and a fitting curve and a correlation coefficient R are output from the fitting result, wherein the correlation coefficient R is used for judging whether the fitting curve is reliable or not.
In one embodiment, combining the response values and parameter values for each known concentration sample into a data set comprises:
transforming the parameter value and the response value of each sample with known concentration into logarithms to form a data set;
or setting the parameter value and the response value of each known concentration sample to the numeric digit of the decimal point to form a data set.
In the embodiment of the present invention, in order to improve the accuracy of the fitting curve, the reaction values and the parameter values of the samples with known concentrations may be preprocessed and then fitted to the fitting curve. The preprocessing comprises the steps of converting the data into a logarithmic form and setting decimal point value digits of the data. Illustratively, transforming the data into a logarithmic form includes: taking logarithm with 10 as base, taking logarithm with natural number as base, taking logarithm with 2 as base, etc.; setting decimal point value digit of data includes: setting the numeric digit of the decimal point to be 1, setting the numeric digit of the decimal point to be 2, setting the numeric digit of the decimal point to be 3 and the like.
In one embodiment, before substituting the response value of each immunoreagent into the fitted curve to obtain the parameter value of the immunoreagent, the method further includes:
setting sampling parameters according to the detection requirements of parameter value detection;
in the embodiment of the present invention, the sampling parameters include a sampling interval, a total sampling time and a single sampling time, where the setting of the sampling interval corresponds to the total sampling time, the sampling interval needs to be set relatively longer when the total sampling time is longer, and the sampling interval needs to be set relatively shorter when the total sampling time is shorter.
Collecting a plurality of original reaction values of the immunological reagent in the detection process according to the sampling parameters;
it is understood that after the detection is completed, a plurality of raw reaction values are collected, and these raw reaction values are reaction values of the immunoreagent at different collection times, and in order to improve the accuracy of the detection of the reaction values, in the embodiment of the present invention, a luminescence curve suitable for the experiment is selected from peak-shaped curves drawn by all raw reaction values, and the reaction value of the immunoreagent is further determined according to the plurality of reaction values in the luminescence curve. For example, a complete reflection value flow combination result is 30 seconds, and a peak shape curve represents a change of a reaction value of the complete flow combination result in 30 seconds, but in an actual detection application, a luminescence curve suitable for an experiment is from 10 seconds to 15 seconds from the start of detection. Wherein, the parameters of the calculation starting point and the calculation end point can be input by a user through a display interface.
Drawing a plurality of original reaction values into a peak-shaped curve, and selecting a section of luminous curve from the peak-shaped curve;
according to the embodiment of the invention, a section of luminous curve in the luminous period is selected from the peak-shaped curves and used for experimental analysis, and the selection method is to determine the end point time of the starting point time of the section of luminous curve through historical detection data and can also be directly selected according to the experience of detection personnel.
The average of several original response values in the luminescence curve was taken as the response value of each immunoreagent.
In the implementation of the invention, the luminous curve comprises a plurality of original reaction values, and the reaction value of the finally tested immune reagent is determined according to the original reaction values by designing and calculating ways, wherein the calculating ways comprise an integration method, an averaging method and the like.
In embodiments of the invention, the calculation for determining the response value of the immunoreagent can be performed in a variety of ways, including averaging, integrating, and the like.
In one embodiment, prior to determining the combined results of the plurality of procedures based on the type of immunoreagent to be developed, the reagent development assay further comprises:
the method comprises the steps of obtaining position information of a plurality of reagents in a detection instrument, and matching the position information of the plurality of reagents with corresponding reagent information.
In the embodiment of the invention, a plurality of reagents are arranged in the detection instrument, each reagent has corresponding reagent information, and the reagent information comprises reagent names, such as reagent A, reagent B, reagent C and the like. The method comprises the steps of setting a certain point in the detection instrument as an origin to construct a coordinate system, representing position information of each reagent by coordinates, matching the position information of the reagent with the reagent information, and calling the required reagent quickly and conveniently according to the position information when the reagent is required to be called in a subsequent flow combination result.
The embodiment of the invention has the following beneficial effects:
according to the method and the device, a plurality of flow combination results are determined according to the type required by the immune reagent to be developed, the flow combination results are sequenced to serve as an experiment sequence, the flow combination results are subjected to experiments according to the experiment sequence to obtain a plurality of immune reagents, and then the immune reagents are subjected to performance tests to determine the target immune reagent with composite preset conditions. The embodiment of the invention takes a plurality of predetermined flow combination results as an experiment sequence to automatically carry out the experiment, can quickly generate a plurality of immunoreagents corresponding to different flow combination results, automatically detects the performance of each immunoreagent to determine the target immunoreagent meeting the preset conditions, not only can effectively improve the efficiency of reagent development, but also can effectively reduce human errors caused by human reagent development, and thus can effectively improve the reliability of reagent development.
Furthermore, the position information of the reagent required by the development of the immunological reagent in the detection instrument is matched with the reagent information, the position information of the reagent can be determined according to the configured reagent information, and the detection instrument can directly call the reagent according to the position information in the detection process, so that the efficiency of reagent development is improved; on the other hand, the configuration of reagent information and the calling information of the reagent can be recorded, the use condition of the reagent on the detection instrument can be monitored through the recorded information, and the reagent can be adjusted according to the use condition of the reagent, so that the convenience of reagent development is facilitated, and different reagent development requirements are favorably met.
Referring to fig. 3, based on the same technical concept as the above embodiment, an embodiment of the present invention provides a reagent development experimental apparatus, including:
a flow combination determination module 10, configured to determine a plurality of flow combination results according to types of immunoreagents to be developed;
the test module 20 is configured to sort the multiple process combination results as an experiment sequence, set the experiment sequence, and perform an experiment on each process combination result according to the experiment sequence;
and the reagent determining module 30 is configured to output an experiment result of the plurality of immunoreagents after the experiment is finished, and determine the target immunoreagent 30 meeting the preset condition from the plurality of immunoreagents according to a performance test result of each immunoreagent.
In one embodiment, the flow combination determination module 10 is configured to:
dividing the flow required by the immunological reagent to be developed according to the type of the immunological reagent to be developed to obtain a plurality of flow sets, wherein each flow set corresponds to one type of immunological reagent to be developed;
and carrying out permutation and combination processing on the flows in each flow set to obtain a plurality of flow combination results.
Referring to FIG. 4, in one embodiment, the reagent determination module 30 includes:
a known concentration sample reaction value detection unit 310, configured to obtain reaction values of a plurality of known concentration samples;
a fitting unit 320 for fitting the reaction values and parameter values of a plurality of samples of known concentration to a fitted curve;
a parameter value obtaining unit 330, configured to substitute the reaction value of each immunoreagent into the fitting curve to obtain a parameter value of the immunoreagent;
the immunoreagent determining unit 340 is configured to determine that the immunoreagent meets a preset condition when the parameter value of the immunoreagent meets the preset condition.
In one embodiment, the immunoreagent determination unit 340 is specifically configured to:
and judging whether the deviation value of the parameter value of the immunological reagent and the preset standard parameter value exceeds a preset threshold range, if not, judging that the parameter value of the immunological reagent meets a preset condition, and judging that the immunological reagent meets the preset condition.
In one embodiment, the known concentration sample response value detection unit 310 is configured to:
judging whether a reagent for detecting the reaction values of the samples with known concentrations is stored in the detection instrument or not according to the position information corresponding to the reagent information, if so, calling the reagent, and detecting by adopting the reagent and configured process parameters to obtain the reaction values of the samples with known concentrations; if not, generating reminding information to remind the user to reconfigure the reagent information.
In one embodiment, the fitting unit 320 is specifically configured to:
forming a data set by the reaction value and the parameter value of each known concentration sample;
and fitting the plurality of data sets into a fitting curve by adopting a preset fitting algorithm.
In one embodiment, combining the response values and parameter values for each known concentration sample into a data set comprises:
transforming the parameter value and the response value of each sample with known concentration into logarithms to form a data set; or
And setting the value digits of decimal points for the parameter value and the reaction value of each known concentration sample to form a data set.
In one embodiment, the parameter value obtaining unit 330 is configured to:
setting sampling parameters according to the detection requirements of parameter value detection;
collecting a plurality of original reaction values of each immunoreagent in the detection process according to the sampling parameters;
drawing a plurality of original reaction values into a peak-shaped curve, and selecting a section of luminous curve from the peak-shaped curve;
the average of several original response values in the luminescence curve was taken as the response value of each immunoreagent.
In one embodiment, the reagent development experimental apparatus further comprises an information matching module, configured to:
the method comprises the steps of obtaining position information of a plurality of reagents in a detection instrument, and matching the position information of the plurality of reagents with corresponding reagent information.
An embodiment of the present invention provides a terminal, including a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor executes the computer program to implement the reagent development experiment method as described above.
One embodiment of the present invention provides a computer-readable storage medium including a stored computer program; wherein the computer program, when executed, controls the apparatus in which the computer readable storage medium is located to perform the reagent development experimental method as described above.
The foregoing is a preferred embodiment of the present invention, and it should be noted that modifications and embellishments could be made by those skilled in the art without departing from the principle of the present invention, and these modifications and embellishments are also regarded as the scope of the present invention.
Claims (20)
1. A reagent development assay method, comprising:
determining a plurality of process combination results according to the type of the immunological reagent to be developed;
sequencing a plurality of flow combination results to serve as an experiment sequence, setting the experiment sequence and carrying out experiments on each flow combination result according to the experiment sequence;
and outputting the experimental results of a plurality of immunoreagents after the experiment is finished, and determining a target immunoreagent meeting preset conditions from the plurality of immunoreagents according to the performance test result of each immunoreagent.
2. The reagent development experimental method of claim 1, wherein determining a plurality of process combination results according to the type of the immunological reagent to be developed comprises:
dividing the flow required by the immunological reagent to be developed according to the type of the immunological reagent to be developed to obtain a plurality of flow sets, wherein each flow set corresponds to one type of immunological reagent to be developed;
and carrying out permutation and combination processing on the processes in each process set to obtain a plurality of process combination results.
3. The reagent development experimental method of claim 1, wherein the step of determining a target immunoreagent meeting the preset condition from a plurality of immunoreagents according to the performance test result of each immunoreagent comprises:
obtaining reaction values of a plurality of samples with known concentrations;
fitting the reaction values and parameter values of a plurality of the samples of known concentration to a fitted curve;
substituting the reaction value of each immunological reagent into the fitting curve to obtain the parameter value of the immunological reagent;
and when the parameter value of the immunological reagent meets the preset condition, judging that the immunological reagent meets the preset condition.
4. The reagent development experimental method according to claim 3, wherein the determining that the immunoreagent satisfies the predetermined condition when the parameter value of the immunoreagent satisfies the predetermined condition includes:
and judging whether the deviation value of the parameter value of the immunological reagent and a preset standard parameter value exceeds a preset threshold range, if not, judging that the parameter value of the immunological reagent meets a preset condition, and judging that the immunological reagent meets the preset condition.
5. The reagent development experimental method according to claim 3, wherein the setting of the experimental order and the experiment of the combined result of each of the procedures according to the experimental order comprise:
judging whether a reagent for detecting the reaction value of the sample with the known concentration is stored in the detection instrument or not according to the position information corresponding to the reagent information, if so, calling the reagent, and detecting by adopting the reagent and the configured process parameters to obtain the reaction values of the samples with the known concentration; if not, generating reminding information to remind the user to reconfigure the reagent information.
6. The reagent development assay of claim 3 wherein said fitting reaction values and parameter values for a plurality of said samples of known concentration to a fitted curve comprises:
combining the reaction value and the parameter value of each known concentration sample into a data set;
and fitting a plurality of data sets into a fitting curve by adopting a preset fitting algorithm.
7. The reagent development assay of claim 6 wherein said combining the values of response and parameter values for each of said samples of known concentration into a data set comprises:
transforming the parameter value and the response value of each known concentration sample into logarithms to form a data set; or
And setting the value digits of decimal points for the parameter value and the response value of each known concentration sample to form a data set.
8. The reagent development assay of claim 3, further comprising, prior to substituting the response value of each of the immunoreagents into the fitted curve to obtain the parameter value of the immunoreagent:
setting sampling parameters according to the detection requirements of parameter value detection;
collecting a plurality of original reaction values of each immunoreagent in the detection process according to the sampling parameters;
drawing a plurality of original reaction values into a peak-shaped curve, and selecting a section of luminous curve from the peak-shaped curve;
taking the average of several original reaction values in the luminescence curve as the reaction value of each immunoreagent.
9. The reagent development assay of claim 1, further comprising, prior to determining the combined results of the plurality of procedures based on the type of immunoreagent to be developed:
the method comprises the steps of obtaining position information of a plurality of reagents in a detection instrument, and matching the position information of the plurality of reagents with corresponding reagent information.
10. A reagent development experimental apparatus, comprising:
the flow combination determining module is used for determining a plurality of flow combination results according to the types of the immunological reagents to be developed;
the test module is used for sequencing a plurality of flow combination results to serve as an experiment sequence, setting the experiment sequence and carrying out experiments on the flow combination results according to the experiment sequence;
and the reagent determining module is used for outputting the experiment results of a plurality of immunoreagents after the experiment is finished, and determining the target immunoreagent meeting the preset conditions from the plurality of immunoreagents according to the performance test result of each immunoreagent.
11. The reagent development experimental apparatus of claim 10, wherein the process combination determination module is configured to:
dividing the flow required by the immunological reagent to be developed according to the type of the immunological reagent to be developed to obtain a plurality of flow sets, wherein each flow set corresponds to one type of immunological reagent to be developed;
and carrying out permutation and combination processing on the processes in each process set to obtain a plurality of process combination results.
12. The reagent development experimental device of claim 10 wherein the reagent determination module comprises:
the reaction value detection unit of the known concentration samples is used for acquiring the reaction values of a plurality of known concentration samples;
a fitting unit for fitting the reaction values and parameter values of a plurality of samples of known concentration to a fitted curve;
the parameter value acquisition unit is used for substituting the reaction value of each immunological reagent into the fitting curve to acquire the parameter value of the immunological reagent;
and the immune reagent judging unit is used for judging that the immune reagent meets the preset condition when the parameter value of the immune reagent meets the preset condition.
13. The reagent development experimental apparatus of claim 12, wherein the immunoreagent determination unit is specifically configured to:
and judging whether the deviation value of the parameter value of the immunological reagent and a preset standard parameter value exceeds a preset threshold range, if not, judging that the parameter value of the immunological reagent meets a preset condition, and judging that the immunological reagent meets the preset condition.
14. The reagent development experimental apparatus of claim 12, wherein the known concentration sample reaction value detection unit is configured to:
judging whether a reagent for detecting the reaction value of the sample with the known concentration is stored in a detection instrument or not according to the position information corresponding to the reagent information, if so, calling the reagent, and detecting by adopting the reagent and configured process parameters to obtain the reaction values of the samples with the known concentration; if not, generating a reminding message to remind the user to reconfigure the reagent information.
15. The reagent development experimental apparatus of claim 12, wherein the fitting unit is specifically configured to:
combining the reaction value and the parameter value of each known concentration sample into a data set;
and fitting a plurality of data sets into a fitting curve by adopting a preset fitting algorithm.
16. The reagent development assay device of claim 15, wherein said combining the response values and parameter values for each of said samples of known concentration into a data set comprises:
transforming the parameter value and the response value of each known concentration sample into logarithms to form a data set; or
And setting the value digits of decimal points for the parameter value and the response value of each known concentration sample to form a data set.
17. The reagent development experimental apparatus of claim 12, wherein the parameter value acquisition unit is configured to:
setting sampling parameters according to the detection requirements of parameter value detection;
collecting a plurality of original reaction values of each immunoreagent in the detection process according to the sampling parameters;
drawing a plurality of original reaction values into a peak-shaped curve, and selecting a section of luminous curve from the peak-shaped curve;
taking the average of several original reaction values in the luminescence curve as the reaction value of each immunoreagent.
18. The reagent development experimental device of claim 10 further comprising an information matching module for:
the method comprises the steps of obtaining position information of a plurality of reagents in a detection instrument, and matching the position information of the plurality of reagents with corresponding reagent information.
19. A terminal comprising a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor, when executing the computer program, implements the reagent development experimentation method of any one of claims 1 to 9.
20. A computer-readable storage medium, characterized in that the computer-readable storage medium comprises a stored computer program; wherein the computer program, when executed, controls an apparatus in which the computer-readable storage medium is located to perform a method of performing a reagent development experiment as claimed in any one of claims 1 to 9.
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