CN115252501B

CN115252501B - Acne-removing composition, preparation method and application

Info

Publication number: CN115252501B
Application number: CN202211036165.5A
Authority: CN
Inventors: 敖德平
Original assignee: Guangzhou Bozhiyue Fine Chemical Co ltd
Current assignee: Guangzhou Bozhiyue Fine Chemical Co ltd
Priority date: 2022-08-27
Filing date: 2022-08-27
Publication date: 2023-06-09
Anticipated expiration: 2042-08-27
Also published as: CN115252501A

Abstract

The invention relates to the field of skin care products, and particularly discloses a acne-removing composition, a preparation method and application thereof. An anti-acne composition, which comprises the following components: cortex Phellodendri extract, plumbum Preparatium extract, usnea barbata extract, herba Artemisiae Annuae extract, salix alba bark extract, radix Sophorae Flavescentis extract, lysozyme, and water. A process for preparing an anti-acne composition comprising the steps of: step 1), putting cortex phellodendri extract, blood circulation promoting pellet extract, usnea barbata extract, sweet wormwood extract, white willow bark extract, kuh-seng root extract and water into a container, continuously stirring, injecting air, controlling the pressure of the container to be 1.8-2.1Mpa, and keeping the temperature at 40-42 ℃ for 8-10 hours; and 2) cooling to 30-32 ℃, adding lysozyme, and uniformly stirring to obtain the acne-removing composition. The invention has the effects of improving the acne removing effect and prolonging the persistence of the acne removing.

Description

Acne-removing composition, preparation method and application

Technical Field

The invention relates to the field of skin care products, in particular to a acne-removing composition, a preparation method and application.

Background

Acne is a chronic inflammatory skin disease of the pilosebaceous glands, which occurs mainly in connection with the factors of hypersecretion of sebum, blockage of the pilosebaceous ducts, bacterial infection, and inflammatory reaction.

Clinical manifestations of vaccinia are mainly acne, pimple, pustule, nodule, etc. which are well developed on the face, and can cause the aesthetic degree of the facial skin to be greatly reduced, and seriously affect the psychological emotion of people.

Based on the pursuit of people on beauty, the treatment of acnes is a hot topic, and along with technological development, acne-removing products are layered endlessly, wherein the acne-removing products by natural plant components are favored by consumers, and the acne-removing products are mainly characterized in that the natural plant components are generally low in skin irritation, low in toxin and not easy to generate negative effects after long-term use.

In the existing acne-removing product, natural plant components with anti-inflammatory and bactericidal effects are mainly added, so that the acne-removing product has the effects of diminishing inflammation and sterilizing, bacteria in the acnes are killed, bacterial reproduction is inhibited, bacterial metabolites are reduced, and the anti-inflammatory effects are assisted, so that the acnes are gradually resolved.

Although the sterilization and bacteriostasis can make the acne subside, the acne can be cleared up, the bacteria can reproduce again after the sterilization and anti-inflammatory effects disappear, so that the acne is easy to recur after the acne subsides, the dependence on the acne removal product is heavy and needs to be used continuously, and the tolerance is easy to generate in the continuous reproduction process of the bacteria, so that the acne removal effect of the acne removal product is gradually reduced after the acne removal product is used for a period of time, and new acne removal products need to be replaced continuously, which is very troublesome, so that the acne removal product has room for improvement.

Disclosure of Invention

In order to improve the acne removing effect and prolong the acne removing durability, the application provides an acne removing composition, a preparation method and application.

In a first aspect, the present application provides an anti-acne composition, which adopts the following technical scheme:

the acne-removing composition comprises the following components in parts by mass:

4-5 parts of cortex phellodendri extract;

3.5-4.5 parts of blood activating pill extract;

3-4 parts of Usnea barbata extract;

2.5-3.5 parts of sweet wormwood herb extract;

4.5-5.5 parts of white willow bark extract;

4.5-5.5 parts of kuh-seng root extract;

0.15-0.25 part of lysozyme;

71.75-77.85 parts of water.

By adopting the technical scheme, the cortex phellodendri extract, the blood circulation activating pellet extract, the Usnea barbata extract, the sweet wormwood extract, the white willow bark extract and the radix sophorae flavescentis extract are matched with each other, so that the acne-removing composition has a good sterilization effect, and particularly has a good sterilization and inhibition effect on staphylococcus aureus; the traditional Chinese medicine composition also has the effects of diminishing inflammation, calming and promoting wound healing, effectively reduces discomfort of skin and accelerates the removal of acnes; the anti-aging agent also has a certain anti-oxidation effect, so that the anti-aging effect of the skin is improved, the acne is not easy to deteriorate, and the acne is easier to heal; in addition, the active ingredients in the cortex phellodendri extract, the blood circulation promoting pellet extract, the Usnea barbata extract, the sweet wormwood extract, the white willow bark extract and the kuh-seng root extract are matched according to a specific proportion, the effect of inhibiting sebum can be better after penetrating into a sebum layer, the secretion of grease is controlled, so that bacteria in vaccinia on the surface are killed and inhibited, the sebum secretion is synchronously reduced to pinch off a channel for providing nutrients for the bacteria, and the bacteria are killed from the front, meanwhile, the grain channel of the bacteria is destroyed from the back, after the sterilization effect is weakened, the bacteria cannot be quickly propagated due to lack of grease as the nutrients, so that the number of the bacteria in the vaccinia is reduced when the acne-removing product is used for continuing acne removal next time, more thorough acne removal can be realized easily, moreover, as sterilization is aimed at the bacterial reproduction process, the bacterial reproduction speed is very fast, so that the persistent acne removal is difficult to realize by simple sterilization, but the skin grease secretion is dependent on the physiological state of the skin, and the effect of inhibiting the grease secretion is more durable by regulating the skin state, so that the effect of permanently cutting off the nutrition supply of bacteria can be realized, and the bacterial reproduction speed is inhibited.

Preferably, the mass ratio of the cortex phellodendri extract, the blood circulation promoting pellet extract, the usnea barbata extract, the sweet wormwood extract, the white willow bark extract and the radix sophorae flavescentis extract is 4.5:4:3.5:3:5:5.

by adopting the technical scheme, the mass ratio of the cortex phellodendri bark extract, the blood circulation promoting pellet extract, the cortex usnea with beard extract, the sweet wormwood extract, the white willow bark extract and the radix sophorae flavescentis extract is specifically selected, so that the effective components in the cortex phellodendri bark extract, the blood circulation promoting pellet extract, the cortex usnea with beard extract, the sweet wormwood extract, the white willow bark extract and the radix sophorae flavescentis extract are matched in an optimal ratio, the interaction effect is better, the oil control effect is better after the cortex phellodendri bark extract, the cortex usnea with beard extract, the cortex phellodendri extract, the radix sophorae flavescentis extract are absorbed by skin, the sebaceous glands are difficult to secrete grease, the skin is drier, vaccinia recurrence is difficult to cause, and the vaccinia treatment effect is more thorough.

Preferably, the extraction method of the cortex phellodendri extract comprises the following steps:

step 1), crushing cortex phellodendri into particles with the particle size of 3-5mm, putting the cortex phellodendri into a container, putting water with the mass of 3-5 times of that of the cortex phellodendri into the container, heating to 42-45 ℃, putting a fermentation composition with the mass of 0.5-0.6% of that of the cortex phellodendri into the container, keeping the temperature at 42-45 ℃, soaking for 24-26h, keeping the rotating speed at 30r/min, stirring for 5min, standing, keeping the temperature at 42-45 ℃ and soaking for 24-26h;

Step 2), heating to boiling, and keeping for 30-35min;

step 3), filtering and taking filtrate;

step 4), heating the filtrate to boiling, and concentrating into paste;

step 5), drying the paste at 120-130 ℃ to obtain dry powder, and obtaining the cortex phellodendri extract;

the fermentation composition is a compound of lactobacillus, saccharomycetes and aspergillus oryzae, wherein the mass ratio of the lactobacillus to the saccharomycetes to the aspergillus oryzae is 0.2:0.3:1.

according to the technical scheme, lactic acid bacteria, saccharomycetes and aspergillus oryzae are adopted to be matched in a specific proportion, the cortex phellodendri can be extracted pertinently, the extraction effect is good, the cortex phellodendri contains lignin, effective substances in the cortex phellodendri are difficult to extract, the lignin can be decomposed by adopting a specific fermentation extraction method, so that cell walls are decomposed better, the effective substances are released into water thoroughly, meanwhile, the lignin is fermented by a specific strain, and the produced fermentation product is matched with the original effective substances in the cortex phellodendri, so that the acne removing effect can be better matched with components in the lignin to improve, the sebum secretion inhibiting effect is especially better, the durability of sebum secretion inhibition is better, the acne removing effect is more thorough, and acne is less prone to relapse.

Preferably, the extraction method of the blood circulation activating pellet extract comprises the following steps:

step 1), putting the blood-activating pill into a container, adding water with the mass 5-6 times of that of the blood-activating pill into the container, soaking for 2-3 hours at normal temperature, filtering out the blood-activating pill, and recovering filtrate for standby;

step 2), putting the blood-activating pill filtered in the step 1) into an environment of-18 ℃ to-20 ℃ and freezing for 6-7h;

step 3), heating the filtrate recovered in the step 1) to boiling, then adding the blood-activating pellet frozen in the step 2), stirring for 30min at the rotating speed of 30-60 r/min;

step 4), cooling to 60-65 ℃, keeping the temperature constant, sealing the container, injecting air, and keeping the pressure in the container at 0.3-0.4MPa for 48-52h;

step 5), filtering out blood circulation activating pills, and heating and concentrating the filtrate to form paste;

step 6), drying the paste at 120-130 ℃ to be powder, and obtaining the blood activating pellet extract.

Through adopting above-mentioned technical scheme, through soaking the back, the red lead that activates blood is close full in water content, then freeze at low temperature, make the moisture in the red lead that activates blood's the cell freeze, form the ice crystal, utilize the volume expansion in the freezing in-process to cooperate with the spike that the ice crystal formed in-process, make the ice crystal puncture the cell wall, thereby make the cell wall form certain damage, then cooperate the filtrate of boiling to heat the red lead that activates blood after freezing rapidly, make the ice crystal vaporization, expand rapidly, thereby thoroughly destroy the cell wall, make the active ingredient in the cell thoroughly release, the extraction effect is better, active material content is higher, better cooperate with other components in order to realize better acne removal, the efficiency of accuse oil.

Preferably, the method for extracting the usnea barbata extract comprises the following steps:

step 1), putting the usnea with the root into a container, adding water with the weight being 2-3 times that of the usnea with the root into the container, leaching for 6-8 hours at 45-50 ℃, filtering, and carrying out solid-liquid separation to obtain primary filter residues and primary filtrate for later use;

step 2), putting primary filter residues into a container, adding water with the mass 2-3 times of that of the primary filter residues into the container, heating and keeping the temperature at 45-50 ℃, sealing the container, injecting air, controlling the pressure in the container to be 1-1.2Mpa, soaking and extracting for 24-26h, performing filter pressing under 10-12Mpa, and performing solid-liquid separation to obtain secondary filtrate for later use;

step 3), combining the secondary filtrate with the primary filtrate, and heating and concentrating the mixture to paste;

step 4), drying the paste at 120-130 ℃ to obtain the usnea barbata extract.

Through adopting above-mentioned technical scheme, through leaching under 1-1.2MPa makes the usnea cell of must be more easily damaged under the pressure, the active substance in the cell releases better, the high pressure of cooperation 10-12MPa carries out the filter-pressing again, make the active ingredient that is remained in the cell release because of the extrusion, the cell wall has very fragile under soaking for a long time and the pressure, under the physical effect of high pressure, can thoroughly destroy, the active substance flows along with the filtrate, extraction efficiency is higher, better cooperation with other substances, it is better to make the sterilization of product, the efficiency of controlling oil better, the effect of treating the vaccinia is better and more thoroughly.

Preferably, the extraction method of the sweet wormwood herb extract comprises the following steps:

step 1), sun-drying sweet wormwood, and then putting the sweet wormwood into a wall breaking machine for breaking wall and crushing to obtain sweet wormwood powder;

step 2), putting sweet wormwood powder into a container, adding water with the mass of 1.5-2 times of that of the sweet wormwood powder, continuously stirring at 100-120r/min, heating to 40-42 ℃, keeping the temperature for 12-14h, filtering, and carrying out solid-liquid separation to obtain primary filter residues and primary filtrate for later use;

step 3), putting the primary filter residue into a container, adding water with the mass which is 2-2.5 times that of the primary filter residue, continuously stirring at 100-120r/min, heating to 45-46 ℃, keeping the temperature for 24-26h, and performing filter pressing at 4-4.5Mpa to obtain secondary filtrate;

step 4), combining the primary filtrate and the secondary filtrate, and heating and concentrating the mixture to form paste;

step 5), drying the paste at 120-130 ℃ to obtain sweet wormwood herb extract.

Through adopting above-mentioned technical scheme, through leaching after broken wall machine broken wall, the extraction effect is better, and the cooperation filter-pressing again to through selecting specific filter-pressing pressure, make the filter residue can not excessively destroy, form certain filtering action through the filter residue, extract required composition better, reduce impurity, it is better to improve the effect of disinfecting, accuse oil with other component cooperation, and the acne is more thorough, and the restoration is faster, and the vaccinia is difficult for recurrence.

Preferably, the method for extracting the white willow bark extract comprises the following steps:

step 1), crushing white willow bark into particles with the particle size of 3-5mm, then putting the white willow bark into a container, then putting water with the mass 3-5 times of that of the white willow bark into the container, heating to 42-45 ℃, then putting a fermentation composition with the mass 0.7-0.8% of that of the white willow bark into the container, keeping the temperature at 42-45 ℃, soaking for 24-26h, keeping the rotating speed at 30r/min, stirring for 5min, standing, keeping the temperature at 42-45 ℃ and soaking for 24-26h;

step 2), heating to boiling, and keeping for 30-35min;

step 3), filtering and taking filtrate;

step 4), heating the filtrate to boiling, and concentrating into paste;

step 5), drying the paste at 120-130 ℃ to obtain dry powder, and obtaining white willow bark extract;

the fermentation composition is a compound of bacillus subtilis, saccharomycetes and trichoderma viride, and the mass ratio of the bacillus subtilis to the saccharomycetes to the trichoderma viride is 0.5:0.2:0.8.

through adopting above-mentioned technical scheme, through adopting bacillus subtilis, microzyme, green trichoderma to ferment and extract the white willow bark with specific proportion cooperation, the pertinence is strong, can fast decomposition white willow bark lignin and cell wall for active ingredient release better, and the extraction efficiency is better, and fermentation product cooperates the back with active ingredient, and the accuse oil effect that forms with other components is better, and the effect of treatment vaccinia is more thorough, and the vaccinia is more difficult for recurrence.

Preferably, the extraction method of the kuh-seng root extract comprises the following steps:

step 1), putting radix sophorae flavescentis into a wall breaking machine for breaking wall and crushing into radix sophorae flavescentis powder, putting the radix sophorae flavescentis powder into a container, adding water with the mass 5-6 times of that of the radix sophorae flavescentis powder, heating and keeping the temperature at 42-45 ℃ and the rotating speed at 60-70r/min, stirring for 12-14h, filtering, and carrying out solid-liquid separation to obtain primary filter residues and primary filtrate;

step 2), putting the primary filter residue into a container, adding water which is 3-4 times the mass of the primary filter residue, heating to boiling, condensing and refluxing, continuously heating for 5-6h, and filtering to obtain secondary filtrate;

step 3), combining the primary filtrate and the secondary filtrate, and heating and concentrating the mixture into paste;

step 4), drying the paste at 120-130 ℃ to form powder, and obtaining the kuh-seng root extract.

By adopting the technical scheme, the wall breaking machine is used for breaking the wall and matching with leaching, then the wall is matched with boiling state for extraction, the extraction effect is good, the oil control effect can be better improved after the extracted active ingredients are matched with other components, the sebum release can be better inhibited, and the vaccinia recurrence can be more thoroughly inhibited.

In a second aspect, the present application provides a method for preparing a parapoxy composition, which adopts the following technical scheme:

The preparation method of the acne-removing composition is characterized by comprising the following steps of: the method comprises the following steps:

step 1), putting cortex phellodendri extract, blood circulation promoting pellet extract, usnea barbata extract, sweet wormwood extract, white willow bark extract, kuh-seng root extract and water into a container, continuously stirring, injecting air, controlling the pressure of the container to be 1.8-2.1Mpa, and keeping the temperature at 40-42 ℃ for 8-10 hours;

and 2) cooling to 30-32 ℃, adding lysozyme, and uniformly stirring to obtain the acne-removing composition.

By adopting the technical scheme, the components which can effectively control oil are formed by mixing the components at the temperature of between 1.8 and 2.1MPa and the temperature of between 40 and 42 ℃ and react with each other under the regulation of special environment, and the components have stronger sterilization, anti-inflammation and repairing effects, can quickly remove acnes after being prepared into skin care products, and have better persistence of the acne removal effect.

In a third aspect, the present application provides an application of an anti-acne composition, which adopts the following technical scheme:

use of a composition for treating acne characterized by: the acne-removing composition is used for preparing skin care products, and the addition amount of the acne-removing composition in the skin care products is 0.5-2% of the total mass of the skin care products.

By adopting the technical scheme, the acne treatment agent has the advantages of better acne treatment effect, no stimulation to skin, lasting oil control effect, difficult recurrence of acnes, and better and thorough acne treatment effect by specifically selecting the addition amount.

In summary, the present application has the following beneficial effects:

1. the cortex phellodendri extract, the blood circulation promoting pellet extract, the usnea barbata extract, the sweet wormwood extract, the white willow bark extract and the radix sophorae flavescentis extract are matched with each other, so that the acne-removing composition has a good sterilization effect, and particularly has a good killing and inhibiting effect on staphylococcus aureus; the traditional Chinese medicine composition also has the effects of diminishing inflammation, calming and promoting wound healing, effectively reduces discomfort of skin and accelerates the removal of acnes; the anti-aging agent also has a certain anti-oxidation effect, so that the anti-aging effect of the skin is improved, the acne is not easy to deteriorate, and the acne is easier to heal; in addition, the acne cream also has the effects of better inhibiting sebum, controlling grease secretion, having better acne removing effect, better skin repairing effect and higher repairing efficiency, and the acne is not easy to relapse, and the acne treating effect is more thorough.

2. In the application, the specific extraction method is preferably adopted for each extract, so that the acne-removing effect of the acne-removing composition is better and the effect is longer.

3. According to the method, the active ingredients react with each other under the regulation of a special environment by mixing at the temperature of between 1.8 and 2.1MPa and at the temperature of between 40 and 42 ℃, so that the ingredients capable of effectively controlling oil are formed, the effects of sterilization, anti-inflammation and repair are strong, and after the skin care product is prepared, acne can be quickly removed, and the persistence of the acne removal effect is better.

Detailed Description

The present application is described in further detail below with reference to examples.

Example 1

An anti-acne composition, which comprises the following components:

cortex Phellodendri extract, plumbum Preparatium extract, usnea barbata extract, herba Artemisiae Annuae extract, salix alba bark extract, radix Sophorae Flavescentis extract, lysozyme, and water.

The preparation method of the cortex phellodendri extract comprises the following steps:

step 1), crushing cortex phellodendri into particles with the particle size of 4+/-1 mm by a crusher, weighing 10kg of cortex phellodendri, putting the particles into an extraction tank, putting 30kg of water into the extraction tank, heating to 42 ℃, putting 0.05kg of fermentation composition into the cortex phellodendri, soaking for 26 hours at the constant temperature of 42 ℃, stirring for 5 minutes at the rotating speed of 30r/min, stopping stirring, standing, keeping the constant temperature of 42 ℃, and soaking for 26 hours.

And 2) heating the liquid in the extraction tank to boiling, and keeping the boiling state for 30min.

And 3) filtering through 200-mesh filter cloth, discarding filter residues, and taking filtrate.

And 4) heating the filtrate to boiling, and concentrating the filtrate into thick paste.

Step 5), putting the paste into a baking oven, and drying the paste into dry powder at 120 ℃ to obtain the cortex phellodendri extract.

Wherein the fermentation composition is a compound of lactobacillus, saccharomycetes and aspergillus oryzae, and the mass ratio of the lactobacillus to the saccharomycetes to the aspergillus oryzae is 0.2:0.3:1.

The lactobacillus is Lactobacillus plantarum purchased from Siemens Qinyuan biotechnology limited company, and the content of viable bacteria is 100 hundred million cfu/g.

The saccharomycete is Blakeslea saccharomycete purchased from LithoTai source biotechnology Co., ltd, and the live bacteria content is 200 hundred million cfu/g.

Aspergillus oryzae was purchased from Guangzhou true microbiological technologies Inc., and had a viable count of 100 hundred million cfu/g.

The preparation method of the blood activating pill extract comprises the following steps:

step 1), 10kg of blood-activating pellet is put into an extraction tank, 50kg of water with the mass of the blood-activating pellet is added into the extraction tank, the water is soaked for 2 to 3 hours at normal temperature, then the blood-activating pellet is filtered out through a filter screen, and the filtrate is recovered for standby.

And 2) putting the blood-activating pill filtered in the step 1) into a freezing chamber at the temperature of minus 18 ℃ and freezing for 7h.

And 3) adding the filtrate recovered in the step 1) into the extract, heating to boil, adding the frozen blood-activating pellet in the step 2) into an extraction tank, stirring for 30min at the rotating speed of 30 r/min.

And 4) cooling the liquid in the extraction tank to 60 ℃, keeping the temperature constant, sealing the extraction tank, injecting air, raising the pressure in the extraction tank to 0.3MPa, and keeping for 52 hours.

Step 5), filtering out the blood circulation promoting pill through a filter screen, and keeping the filtrate in an extraction tank, and heating and concentrating the filtrate to form thick paste.

And 6) placing the paste into an oven, and drying the paste to be powder at 120 ℃ to obtain the blood activating pellet extract.

The preparation method of the Usnea barbata extract comprises the following steps:

step 1), putting 10kg of usnea with whisker into an extraction tank, adding 20kg of water into the extraction tank, leaching for 8 hours at 45 ℃, filtering by a filter screen, and carrying out solid-liquid separation to obtain primary filter residues and primary filtrate for later use.

And 2) putting primary filter residues into an extraction tank, adding 20kg of water into the extraction tank, heating the water to 45 ℃ and keeping the temperature constant, sealing the container, injecting air, controlling the pressure in the container to be 1Mpa, soaking and extracting for 26h, performing filter pressing under 10Mpa by a filter press, and performing solid-liquid separation to obtain secondary filtrate for later use.

And 3) combining the secondary filtrate with the primary filtrate, and heating and concentrating the mixture to obtain a thick paste.

And 4) placing the paste into an oven, and drying the paste into powder at 120 ℃ to obtain the Usnea barbata extract.

The preparation method of the sweet wormwood herb extract comprises the following steps:

step 1), sun-drying the sweet wormwood, and putting the sun-dried sweet wormwood into a wall breaking machine for breaking wall and crushing to obtain sweet wormwood powder.

And 2), weighing 10kg of sweet wormwood powder, putting into an extraction tank, adding 15kg of water, stirring continuously at 100r/min, heating to 40 ℃, keeping the temperature for 14 hours, filtering by a screen, and carrying out solid-liquid separation to obtain primary filter residues and primary filtrate for later use.

And 3) putting the primary filter residues into an extraction tank, adding 20kg of water, continuously stirring at 100r/min, heating to 45 ℃, keeping the temperature for 26 hours, and performing 4Mpa filter pressing by a filter press to obtain secondary filtrate.

And 4) combining the primary filtrate and the secondary filtrate, and heating and concentrating to form thick paste.

Step 5), drying the paste at 120 ℃ to obtain the sweet wormwood herb extract.

The preparation method of the white willow bark extract comprises the following steps:

step 1), crushing white willow bark into particles with the particle size of 3mm by a crusher, weighing 10kg of white willow bark particles, putting the particles into an extraction tank, then putting 30kg of water into the extraction tank, heating to 42 ℃, then putting 0.07kg of fermentation composition, soaking for 26 hours at the constant temperature of 42 ℃, stirring for 5 minutes at the rotating speed of 30r/min, stopping stirring, standing, and soaking for 26 hours at the constant temperature of 42 ℃.

And 2) heating until the liquid in the extraction tank boils, and keeping the boiling state for 30min.

Step 3), filtering through 200-mesh filter cloth, and taking filtrate.

And 5) placing the paste into an oven, and drying the paste into dry powder at 120 ℃ to obtain the white willow bark extract.

Wherein the fermentation composition is a compound of bacillus subtilis, saccharomycetes and trichoderma viride, and the mass ratio of the bacillus subtilis to the saccharomycetes to the trichoderma viride is 0.5:0.2:0.8.

bacillus subtilis is purchased from Shandong Weiduofeng biotechnology Co., ltd, and the viable bacteria content is 200 hundred million cfu/g.

Trichoderma viride was purchased from North Biotechnology Co., ltd. Shandong, and the viable bacteria content was 100 hundred million cfu/g.

The preparation method of the kuh-seng root extract comprises the following steps:

step 1), putting radix sophorae flavescentis into a wall breaking machine for breaking wall and crushing the radix sophorae flavescentis into radix sophorae flavescentis powder, weighing 10kg of radix sophorae flavescentis powder, putting the radix sophorae flavescentis powder into an extraction tank, adding 50kg of water, heating and keeping the temperature at 42 ℃, stirring for 14h at the rotating speed of 60r/min, filtering with 200-mesh filter cloth, and carrying out solid-liquid separation to obtain primary filter residues and primary filtrate.

And 2) putting the primary filter residues into an extraction tank, adding 30kg of water, heating until the liquid in the extraction tank is boiled, condensing and refluxing, continuously boiling for 5h, and filtering with 200-mesh filter cloth to obtain secondary filtrate.

And 3) combining the primary filtrate and the secondary filtrate, and heating and concentrating the mixture into thick paste.

And 4) placing the paste into an oven, and drying at 120 ℃ to obtain the kuh-seng root extract.

The preparation method of the anti-acne composition comprises the following steps:

step 1), weighing 40g of cortex phellodendri extract, 35g of blood circulation promoting pill extract, 30g of Usnea barbata extract, 25g of sweet wormwood extract, 45g of white willow bark extract, 45g of radix sophorae flavescentis extract and 778.5g of water, putting into a stirring kettle, continuously stirring at a rotating speed of 30r/min, sealing the stirring kettle, injecting air, controlling the pressure in the kettle to be 1.8Mpa, and keeping the temperature at 40 ℃ for 10 hours.

And 2) cooling to 30 ℃, adding 1.5g of lysozyme, stirring for 5min at the rotating speed of 60r/min, and uniformly stirring to obtain the acne-removing composition.

Example 2

An anti-acne composition, which comprises the following components:

step 1), crushing cortex phellodendri into particles with the particle size of 4+/-1 mm by a crusher, weighing 10kg of cortex phellodendri, putting the particles into an extraction tank, putting 50kg of water into the extraction tank, heating to 45 ℃, putting 0.06kg of fermentation composition into the cortex phellodendri, soaking for 24 hours at the constant temperature of 45 ℃, stirring for 5 minutes at the rotating speed of 30r/min, stopping stirring, standing, keeping the constant temperature of 45 ℃, and soaking for 24 hours.

And 2) heating the liquid in the extraction tank to boiling, and keeping the boiling state for 35min.

Step 5), putting the paste into a baking oven, and drying the paste into dry powder at 130 ℃ to obtain the cortex phellodendri extract.

step 1), 10kg of blood-activating pellet is put into an extraction tank, 60kg of water with the mass of the blood-activating pellet is added into the extraction tank, the water is soaked for 2 to 3 hours at normal temperature, then the blood-activating pellet is filtered out through a filter screen, and the filtrate is recovered for standby.

And 2) putting the blood-activating pill filtered in the step 1) into a freezing chamber at the temperature of minus 20 ℃ and freezing for 6 hours.

And 3) adding the filtrate recovered in the step 1) into the extract, heating to boil, adding the frozen blood circulation promoting pellet in the step 2) into an extraction tank, and stirring for 30min at the rotating speed of 60 r/min.

And 4) cooling the liquid in the extraction tank to 65 ℃, keeping the temperature constant, sealing the extraction tank, injecting air, raising the pressure in the extraction tank to 0.4MPa, and keeping the pressure for 48 hours.

And 6) placing the paste into an oven, and drying the paste to be powder at 130 ℃ to obtain the blood activating pellet extract.

step 1), putting 10kg of usnea with whisker into an extraction tank, adding 30kg of water into the extraction tank, leaching for 6 hours at 50 ℃, filtering by a filter screen, and carrying out solid-liquid separation to obtain primary filter residues and primary filtrate for later use.

And 2) putting primary filter residues into an extraction tank, adding 30kg of water into the extraction tank, heating the water to 50 ℃ and keeping the temperature constant, sealing the container, injecting air, controlling the pressure in the container to be 1.2Mpa, soaking and extracting for 24h, performing filter pressing under 12Mpa by a filter press, and performing solid-liquid separation to obtain secondary filtrate for later use.

And 4) placing the paste into an oven, and drying the paste into powder at 130 ℃ to obtain the Usnea barbata extract.

Step 2), weighing 10kg of sweet wormwood powder, putting into an extraction tank, adding 20kg of water at 120r/min, continuously stirring, heating to 42 ℃, keeping the temperature for 12 hours, filtering by a screen, and carrying out solid-liquid separation to obtain primary filter residues and primary filtrate for later use.

And 3) putting the primary filter residues into an extraction tank, adding 25kg of water, continuously stirring at 120r/min, heating to 46 ℃, keeping the temperature for 24 hours, and performing filter pressing by using a filter press at 4.5Mpa to obtain secondary filtrate.

Step 5), drying the paste at 130 ℃ to obtain the sweet wormwood herb extract.

step 1), crushing white willow bark into particles with the particle size of 5mm by a crusher, weighing 10kg of white willow bark particles, putting the particles into an extraction tank, then putting 50kg of water into the extraction tank, heating to 45 ℃, then putting 0.08kg of fermentation composition, soaking for 24 hours at the constant temperature of 45 ℃, stirring for 5 minutes at the rotating speed of 30r/min, stopping stirring, standing, and soaking for 24 hours at the constant temperature of 45 ℃.

And 2) heating until the liquid in the extraction tank boils, and keeping the boiling state for 35min.

Step 3), filtering through 200-mesh filter cloth, and taking filtrate.

And 5) placing the paste into an oven, and drying the paste into dry powder at 130 ℃ to obtain the white willow bark extract.

step 1), putting radix sophorae flavescentis into a wall breaking machine for breaking wall and crushing into radix sophorae flavescentis powder, weighing 10kg of radix sophorae flavescentis powder, putting into an extraction tank, adding 60kg of water, heating and keeping the temperature at 45 ℃, stirring for 12h at the rotating speed of 70r/min, filtering with 200-mesh filter cloth, and carrying out solid-liquid separation to obtain primary filter residues and primary filtrate.

And 2) putting the primary filter residues into an extraction tank, adding 40kg of water, heating until the liquid in the extraction tank is boiled, condensing and refluxing, continuously boiling for 6 hours, and filtering with 200-mesh filter cloth to obtain secondary filtrate.

And 4) placing the paste into an oven, and drying at 130 ℃ to obtain the kuh-seng root extract.

Example 3

An anti-acne composition which differs from example 1 only in that:

step 1), weighing 45g of cortex phellodendri extract, 40g of blood circulation promoting pill extract, 35g of Usnea barbata extract, 30g of sweet wormwood extract, 50g of white willow bark extract, 50g of radix sophorae flavescentis extract and 748g of water, putting into a stirring kettle, continuously stirring at a rotating speed of 30r/min, sealing the stirring kettle, injecting air, controlling the pressure in the kettle to be 1.8Mpa, and keeping the temperature at 40 ℃ for 10 hours.

And 2), cooling to 30 ℃, adding 2g of lysozyme, stirring for 5min at the rotating speed of 60r/min, and uniformly stirring to obtain the acne-removing composition.

Example 4

An anti-acne composition which differs from example 1 only in that:

step 1), weighing 50g of cortex phellodendri extract, 45g of blood circulation promoting pill extract, 40g of Usnea barbata extract, 35g of sweet wormwood extract, 55g of white willow bark extract, 55g of radix sophorae flavescentis extract and 717.5g of water, putting into a stirring kettle, continuously stirring at a rotating speed of 30r/min, sealing the stirring kettle, injecting air, controlling the pressure in the kettle to be 1.8Mpa, and keeping the temperature at 40 ℃ for 10 hours.

And 2) cooling to 30 ℃, adding 2.5g of lysozyme, stirring for 5min at the rotating speed of 60r/min, and uniformly stirring to obtain the acne-removing composition.

Example 5

An anti-acne composition which differs from example 1 only in that:

step 1), weighing 40g of cortex phellodendri extract, 35g of blood circulation promoting pill extract, 30g of Usnea barbata extract, 25g of sweet wormwood extract, 45g of white willow bark extract, 45g of radix sophorae flavescentis extract and 778.5g of water, putting into a stirring kettle, continuously stirring at a rotating speed of 30r/min, sealing the stirring kettle, injecting air, controlling the pressure in the kettle to be 2.1Mpa, and keeping the temperature at 42 ℃ for 8 hours.

And 2) cooling to 32 ℃, adding 1.5g of lysozyme, stirring for 5min at the rotating speed of 60r/min, and uniformly stirring to obtain the acne-removing composition.

Example 6

An anti-acne composition which differs from example 1 only in that:

the cortex Phellodendri extract prepared by the preparation method of cortex Phellodendri extract in example 1 was replaced with commercial cortex Phellodendri extract.

The commercial cortex phellodendri extract is an aqueous extract, and the ratio of the extract is 10:1.

example 7

An anti-acne composition which differs from example 1 only in that:

the blood-activating pellet extract prepared by the preparation method of the blood-activating pellet extract in example 1 was replaced with the commercially available blood-activating pellet extract in an equal amount.

Commercial blood circulation promoting pill extract is water extract, and the proportion extract is 10:1.

example 8

An anti-acne composition which differs from example 1 only in that:

the Usnea barbata extract prepared by the preparation method of Usnea barbata extract in example 1 was replaced with a commercially available Usnea barbata extract in equal amounts.

The commercial Usnea barbata extract is an aqueous extract with the ratio of 10:1.

example 9

An anti-acne composition which differs from example 1 only in that:

the sweet wormwood extract prepared by the preparation method of the sweet wormwood extract in example 1 was replaced with a commercial sweet wormwood extract in equal amount.

The commercial sweet wormwood herb extract is an aqueous extract, and the ratio of the extract is 10:1.

example 10

An anti-acne composition which differs from example 1 only in that:

the white willow bark extract prepared by the preparation method of the white willow bark extract in example 1 was replaced with the commercially available white willow bark extract in equal amounts.

The commercially available white willow bark extract is an aqueous extract, and the ratio of the extract is 10:1.

example 11

An anti-acne composition which differs from example 1 only in that:

the kuh-seng extract prepared by the preparation method of the kuh-seng extract in example 1 was replaced with the commercial kuh-seng extract in equal amount.

Commercial kuh-seng root extract is water extract, and the ratio of extract is 10:1.

comparative example 1

An anti-acne composition which differs from example 1 only in that:

the cortex Phellodendri bark extract of example 1 was replaced with the commercially available herba Portulacae extract in equal amounts.

The commercially available purslane extract is an aqueous extract, and the ratio of the extract to the water extract is 10:1.

comparative example 2

An anti-acne composition which differs from example 1 only in that:

the extract of Huoxuedan in example 1 was replaced with the commercially available purslane extract in equal amounts.

comparative example 3

An anti-acne composition which differs from example 1 only in that:

the Usnea barbata extract of example 1 was replaced with a commercially available purslane extract in equal amounts.

comparative example 4

An anti-acne composition which differs from example 1 only in that:

the herba Artemisiae Annuae extract of example 1 was replaced with the commercially available herba Portulacae extract in equal amount.

comparative example 5

An anti-acne composition which differs from example 1 only in that:

the white willow bark extract of example 1 was replaced with an equivalent amount of a commercially available purslane extract.

comparative example 6

An anti-acne composition which differs from example 1 only in that:

the kuh-seng extract of example 1 was replaced with a commercial purslane extract in equal amounts.

comparative example 7

An anti-acne composition which differs from example 1 only in that:

step 1), weighing 35g of cortex phellodendri extract, 50g of blood circulation promoting pill extract, 25g of Usnea barbata extract, 20g of sweet wormwood extract, 60g of white willow bark extract, 30g of radix sophorae flavescentis extract and 778.5g of water, putting into a stirring kettle, continuously stirring at a rotating speed of 30r/min, sealing the stirring kettle, injecting air, controlling the pressure in the kettle to be 1.8Mpa, and keeping the temperature at 40 ℃ for 10 hours.

Application example 1

An anti-acne lotion is prepared from anti-acne composition and deionized water.

The preparation method of the anti-acne lotion comprises the following steps:

0.5g of the acne-removing composition and 99.5g of deionized water are weighed, and the acne-removing composition and the deionized water are uniformly mixed to obtain the acne-removing water.

The anti-acne composition was the anti-acne composition of example 1.

Application example 2

An anti-acne lotion is prepared from anti-acne composition and deionized water.

The preparation method of the anti-acne lotion comprises the following steps:

1g of the acne-removing composition and 99g of deionized water are weighed, and the acne-removing composition and the deionized water are uniformly mixed to obtain the acne-removing water.

The anti-acne composition was the anti-acne composition of example 1.

Application example 3

An anti-acne lotion is prepared from anti-acne composition and deionized water.

The preparation method of the anti-acne lotion comprises the following steps:

2g of the acne-removing composition and 98g of deionized water are weighed, and the acne-removing composition and the deionized water are uniformly mixed to obtain the acne-removing water.

The anti-acne composition was the anti-acne composition of example 1.

Application example 4

The only difference between the anti-acne water and the application example 3 is that:

the anti-acne composition was the anti-acne composition of example 2.

Application example 5

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of example 3.

Application example 6

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of example 4.

Application example 7

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of example 5.

Application example 8

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of example 6.

Application example 9

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of example 7.

Application example 10

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of example 8.

Application example 11

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of example 9.

Application example 12

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of example 10.

Application example 13

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of example 11.

Application example 14

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of comparative example 1.

Application example 15

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of comparative example 2.

Application example 16

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of comparative example 3.

Application example 17

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of comparative example 4.

Application example 18

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of comparative example 5.

Application example 19

The only difference between the anti-acne water and the application example 3 is that: the anti-acne composition was the anti-acne composition of comparative example 6.

Application example 20

the anti-acne composition was the anti-acne composition of comparative example 7.

Experiment 1

Oil control efficacy test:

1. test instrument: skin grease tester Sebumeter, CK, germany.

2. The test principle is as follows: based on photometer principle, through the grease on the skin of 0.1mm thick special extinction sticky tape absorption human body, extinction sticky tape can become semitransparent, and the light transmission changes, and the more grease that absorbs, the more the light transmission is, thereby the measurement skin grease content, the numerical value is the bigger, represents the skin grease content is the higher, and the skin oil yield is the greater.

3. Test sample: skin repair essence stock samples prepared in each example and comparative example.

4. The testing method comprises the following steps: selecting subjects with oily skin and acne growing on the face and aged between 20 and 40 years, wherein each 3 people are one group, and after the subjects use the same product with no oil control effect for cleaning the face in the morning and evening every day, applying acne-measuring water on the face area, and using the acne-measuring water of the same application example.

The forehead of the subject was tested for skin fat content by using a skin fat tester Sebumeter before using the test sample and after using the kepoxwater for 7 days and 28 days, and the results of each group were averaged to calculate the skin fat content change rate.

The anti-acne water was discontinued after 28 days of use and the subject was tested for lipid content in the forehead after 14 days, 28 days of discontinuation using a skin lipid tester Sebumeter.

5. And (3) data processing:

skin oil content change rate (%) = (Y1-Y0)/y0×100%

Y0: skin lipid content prior to use of the test sample;

y1: skin oil content after use of the test sample or after inactivation of the sample.

Experiment 2

Acne removing efficacy test:

the testing method comprises the following steps: selecting subjects with oily skin and acne growing on the face between 20 and 40 years old, wherein the number of the acne on the face of each subject is not less than 10, each 3 persons are one group, each subject uses the same product with no oil control and acne removal effects in the morning and evening every day, and then, the acne-removing water is smeared on the face area, and one group of acne-removing water with the same application example is used.

After 7 days of use, the cases of eliminating acnes and recurrence were observed, and after 28 days of use, the cases of eliminating acnes and recurrence were observed.

After 28 days of use, the patients were stopped for 14 and 28 days, and the recurrent cases of acnes were observed again.

The specific detection data of experiments 1 and 2 are shown in tables 1-2.

TABLE 1

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TABLE 2

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According to the data comparison of application examples 3 and 8-13 in tables 1 and 2, when cortex phellodendri extract, blood circulation promoting pellet extract, usnea barbata extract, sweet wormwood extract, white willow bark extract and radix sophorae flavescentis extract are all extracted by adopting a specific extraction method, the acne-eliminating composition has the advantages of quicker effect for eliminating acnes, better oil control effect, less recurrence of acnes after stopping use and more complete and lasting acne elimination.

According to the comparison of the data of application examples 3 and 14-19 in tables 1 and 2, when any one of the cortex phellodendri extract, the blood circulation promoting pellet extract, the Usnea barbata extract, the sweet wormwood extract, the white willow bark extract and the radix sophorae flavescentis extract is replaced, the oil control effect of the acne-removing composition is greatly reduced, so that the acne-removing effect is slow, and mainly because the sterilizing effect is counteracted by the bacterial reproduction speed, the oil is more, the bacterial reproduction capability is stronger, the bacteria cannot be rapidly and thoroughly killed, and the wound healing is slow.

According to the comparison of the data of application examples 3 and 20 in tables 1 and 2, when the cortex phellodendri extract, the blood circulation promoting pellet extract, the usnea barbata extract, the sweet wormwood extract, the white willow bark extract and the radix sophorae flavescentis extract are not mixed in a specific proportion, the acne removing efficiency is reduced, the oil control effect is reduced, and the acne removing effect can be thoroughly and permanently achieved only when the cortex phellodendri extract, the blood circulation promoting pellet extract, the usnea barbata extract, the sweet wormwood extract, the white willow bark extract and the radix sophorae flavescentis extract are mixed in a specific proportion.

The present embodiment is merely illustrative of the present application and is not intended to be limiting, and those skilled in the art, after having read the present specification, may make modifications to the present embodiment without creative contribution as required, but is protected by patent laws within the scope of the claims of the present application.

Claims

1. An anti-acne composition, characterized in that: the composite material consists of the following components in parts by weight:

4-5 parts of cortex phellodendri extract;

3.5-4.5 parts of blood activating pill extract;

3-4 parts of Usnea barbata extract;

2.5-3.5 parts of sweet wormwood herb extract;

4.5-5.5 parts of white willow bark extract;

4.5-5.5 parts of kuh-seng root extract;

0.15-0.25 part of lysozyme;

71.75-77.85 parts of water;

the extraction method of the cortex phellodendri extract comprises the following steps:

step 2), heating to boiling, and keeping for 30-35min;

step 3), filtering and taking filtrate;

step 4), heating the filtrate to boiling, and concentrating into paste;

the fermentation composition is a compound of lactobacillus, saccharomycetes and aspergillus oryzae, wherein the mass ratio of the lactobacillus to the saccharomycetes to the aspergillus oryzae is 0.2:0.3:1, a step of;

the extraction method of the blood circulation activating pellet extract comprises the following steps:

Step 2), putting the blood circulation activating pill filtered in the step 1) into an environment of-18 ℃ to-20 ℃ and freezing for 6-7h;

step 6), drying the paste at 120-130 ℃ to powder to obtain the blood activating pellet extract;

the extraction method of the Usnea barbata extract comprises the following steps:

Step 4), drying the paste at 120-130 ℃ to obtain powder, and obtaining the usnea barbata extract;

the extraction method of the sweet wormwood herb extract comprises the following steps:

step 5), drying the paste at 120-130 ℃ to obtain sweet wormwood herb extract;

the method for extracting the white willow bark extract comprises the following steps:

Step 2), heating to boiling, and keeping for 30-35min;

step 3), filtering and taking filtrate;

step 4), heating the filtrate to boiling, and concentrating into paste;

the fermentation composition is a compound of bacillus subtilis, saccharomycetes and trichoderma viride, and the mass ratio of the bacillus subtilis to the saccharomycetes to the trichoderma viride is 0.5:0.2:0.8;

the extraction method of the kuh-seng root extract comprises the following steps:

2. An anti-acne composition according to claim 1, characterized in that: the mass ratio of the cortex phellodendri extract, the blood circulation promoting pellet extract, the Usnea barbata extract, the sweet wormwood extract, the white willow bark extract and the kuh-seng root extract is 4.5:4:3.5:3:5:5.

3. a process for the preparation of the anti-acne composition according to claim 1 or 2, characterized in that: the method comprises the following steps:

4. Use of the anti-acne composition according to claim 1 or 2 and/or the anti-acne composition prepared by the preparation method according to claim 3 for preparing skin care products, characterized in that: the acne-removing composition is used for preparing skin care products, and the addition amount of the acne-removing composition in the skin care products is 0.5-2% of the total mass of the skin care products.