CN115227865A - Quaternary ammonium salt photo-thermal synergistic antibacterial sports colloid dressing - Google Patents
Quaternary ammonium salt photo-thermal synergistic antibacterial sports colloid dressing Download PDFInfo
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- CN115227865A CN115227865A CN202210876927.6A CN202210876927A CN115227865A CN 115227865 A CN115227865 A CN 115227865A CN 202210876927 A CN202210876927 A CN 202210876927A CN 115227865 A CN115227865 A CN 115227865A
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- 150000003242 quaternary ammonium salts Chemical class 0.000 title claims abstract description 13
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- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 76
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- 238000000034 method Methods 0.000 claims description 6
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- RRHXZLALVWBDKH-UHFFFAOYSA-M trimethyl-[2-(2-methylprop-2-enoyloxy)ethyl]azanium;chloride Chemical compound [Cl-].CC(=C)C(=O)OCC[N+](C)(C)C RRHXZLALVWBDKH-UHFFFAOYSA-M 0.000 claims description 3
- 238000001132 ultrasonic dispersion Methods 0.000 claims description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0095—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/204—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with nitrogen-containing functional groups, e.g. aminoxides, nitriles, guanidines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/204—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with nitrogen-containing functional groups, e.g. aminoxides, nitriles, guanidines
- A61L2300/208—Quaternary ammonium compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
Abstract
The invention relates to a material in the field of medical supplies, in particular to a preparation method of a sports colloid dressing with multi-mode synergistic antibacterial, anti-freezing water retention and biocompatibility. In order to prepare a wound dressing with multi-mode antibacterial, adhesion, freeze-proof water retention and photothermal response by using DMC, SA, dopamine and glycerol as main raw materials, the invention provides a sports colloid dressing which comprises the following components in percentage by mass and volume: and SA: 1-3wt%, DMC:20wt% -30wt%, PDA:1wt% -7wt%, glycerin: 80v/v%, initiator: 1wt%, crosslinking agent: 1wt%. The wound dressing prepared by the invention has excellent biological safety, frost resistance, water retention property and adhesion, integrates the antibacterial and photothermal treatment of quaternary ammonium salt, and has good application prospect.
Description
The technical field is as follows:
the invention relates to a material in the field of medical supplies, in particular to a preparation method of a sports colloid dressing with multi-mode synergistic antibacterial, anti-freezing water retention and biocompatibility.
Technical background:
the skin is the first barrier of the organism and plays an important role in balancing body temperature, regulating metabolism, protecting internal tissues from being invaded by external microorganisms and the like. However, human skin is susceptible toTo various types of injuries such as bruises, cuts, burns, etc. In the self-repairing process of the wound surface, bacterial infection is difficult to avoid, improper treatment can result in suppuration and fester of the wound, aggravation of internal tissue damage, slow wound healing process and continuous pain, and huge physiological and psychological pressure is caused to patients. At present, two types of commonly used antibacterial means are mainly adopted, one type is that hydrogel is taken as a substrate to carry metal ions such as Ag, cu and the like or ZnO and TiO in a complexing way 2 The metal oxide nano particles kill bacteria by utilizing the controllable release of metal ions; the other is to form a high molecular antibacterial film or gel by means of thermal polymerization, photopolymerization and the like by using the quaternary ammonium salt with positive charges. The quaternary ammonium salt antibacterial agent has the characteristics of broad spectrum, long effect and safety, and avoids secondary damage to human bodies caused by the enrichment of heavy metal ions in the bodies.
Methacryloyloxyethyltrimethyl ammonium chloride (DMC), a common quaternary ammonium salt monomer, has a positively charged-NH in its molecular structure 4 + Through the interaction with the negatively charged bacterial membrane, the membrane structure of the bacteria is destroyed, so that nutrients such as protein of the bacterial body are leaked out, and finally the bacteria are killed. Meanwhile, due to the polymerizability of the DMC monomer, a continuous PDMC polymer network can be formed through free radical polymerization, and various types of antibacterial materials such as gel can be further formed.
Compared with DMC excellent antibacterial property, it has the disadvantage of insufficient biocompatibility. Sodium Alginate (SA), a natural high-molecular polysaccharide from plants, has proven its excellent biosafety in the fields of food and medicine. The addition of sodium alginate can improve the biocompatibility of the gel dressing on one hand and improve the mechanical property of the dressing through chain entanglement or hydrogen bond interaction on the other hand. Dopamine (DA), which is an adhesion protein-like substance inspired by mussels, is easily oxidized and polymerized in a weak alkaline environment to form Polydopamine (PDA). PDA has excellent adhesion and photothermal conversion properties, and excellent biosafety is its outstanding advantage compared to conventional photothermal agents (MXene, carbon nanotubes, graphene, etc.). Therefore, PDA can be used as a biologically safe photothermal agent for photothermal therapy.
During the preparation of the gel dressing, free radical polymerization of DMC was hindered due to the free radical quenching properties of PDA. Therefore, PDA and the anti-freezing water-retaining agent glycerol are compounded to form mixed dispersion liquid, and the gel dressing is subjected to functional modification through dynamic solvent replacement.
In conclusion, the SA/DMC-based composite organogel dressing prepared by the quaternary ammonium salt monomer free radical polymerization and dynamic solvent replacement method has excellent biological safety, frost resistance and water retention performance, and integrates the antibacterial and photothermal treatment of quaternary ammonium salt.
The invention content is as follows:
aiming at the problems in the prior art, the invention aims to provide a multifunctional sports colloid dressing which integrates environmental adaptability and high-efficiency antibacterial property, thereby accelerating wound healing.
In a first aspect, the invention is realized by the following technical scheme: the quaternary ammonium salt photo-thermal synergistic antibacterial sports colloid dressing comprises the following components: sodium Alginate (SA), methacryloyloxyethyltrimethylammonium chloride (DMC), polydopamine (PDA), glycerol, potassium peroxodisulfate (KPS), PEGDA400.
The invention provides a composite organic gel dressing with environmental adaptability and photo-thermal synergistic antibacterial quaternary ammonium salt. And (3) initiating DMC polymerization in a sodium alginate solution by using potassium peroxydisulfate as an initiator and PEGDA400 as a cross-linking agent to form SA/DMC gel. And finally, introducing an anti-freezing water retention factor (glycerin) and a biophotonic agent (PDA) by a dynamic solvent replacement method, so as to improve the environmental adaptability of the gel and endow the gel with photo-thermal response performance.
The preferred mass fraction of sodium alginate is 1-3 wt.%, and may be, for example, 1 wt.%, 2 wt.%, 3 wt.%. The mass fraction of DMC is 20 to 30% by weight, and may be, for example, 20% by weight, 25% by weight, or 30% by weight. The mass fraction of the sodium alginate and the DMC refers to the ratio of the mass of the sodium alginate to the total mass of the mixed solution.
In the present invention, the crosslinking agent PEGDA400 is added in an amount of 1wt% based on the mass of the monomer DMC.
In the invention, the glycerol and dopamine solution are used as main components of the dynamic displacement solution, so that the mechanical property of the gel is improved, and the gel can be endowed with frost resistance, water retention and photo-thermal response.
Preferably, the dopamine solution in the replacement solution has a volume fraction of 20-80v/v%, which may be 20v/v%,40v/v%,60v/v%, or 80v/v%.
In a second aspect, the invention provides a preparation method of the quaternary ammonium salt photothermal synergistic antibacterial sports colloid dressing, which specifically comprises the following steps:
specifically, the preparation method of the sports colloid dressing comprises the following steps:
(1) Preparing SA/DMC mixed solution
The DMC solution (80 wt.%) was mixed homogeneously in deionized water. Adding sodium alginate powder into the obtained uniform mixed solution, heating and stirring at 70 ℃ for 5h to obtain uniform SA/DMC mixed solution, and cooling for later use.
(2) Preparation of SA/DMC gel
Adding initiator potassium peroxodisulfate and cross-linking agent PEGDA400 into the SA/DMC mixed solution, performing ultrasonic dispersion, and magnetically stirring to obtain uniform mixed solution. After the vacuum environment is deaerated, the mixed solution is injected into a mold consisting of a glass plate and a rubber pad, and is thermally polymerized for 8 hours at 70 ℃ to obtain SA/DMC gel.
(3) Preparation of Glycerol/PDA Mixed replacement liquid
DA was dissolved in phosphate buffer (pH = 8.5) to prepare solutions of DA (1 wt% to 10 wt%) at different concentrations. PDA chains were generated by stirring at room temperature in an air atmosphere for 30 min. And compounding the obtained dispersion liquid with different mass concentrations and glycerol according to the volume ratio to form the mixed replacement liquid.
(4) Preparation of SA/DMC-based composite organogel
And (3) placing the gel into different mixed replacement liquids at room temperature, placing for 24 hours in the air atmosphere, and wiping the surface of the gel to obtain the composite organic gel.
Description of the drawings:
FIG. 1 shows S in the present inventionA 3 DMC 25 Gel and SA 3 DMC 25 Optical photographs of the base complex organogels.
FIG. 2 shows SA according to the present invention 3 DMC 25 The photo of the composite organic gel with high efficiency and synergistic sterilization under near infrared light.
The specific implementation mode is as follows:
example 1
(1) Preparing SA/DMC mixed solution
3.125g of DMC solution (80 wt.%) was mixed well in 6.575g of deionized water. Adding 0.3g of sodium alginate powder into the obtained uniform mixed solution, heating and stirring at 70 ℃ for 5h to obtain a uniform SA/DMC mixed solution, and cooling for later use.
(2) Preparation of SA 3 DMC 25 Gel
Adding initiator potassium peroxodisulfate (0.025 g) and crosslinking agent PEGDA400 (250 μ L,0.1 g/mL) into the SA/DMC mixed solution, performing ultrasonic treatment for 5min, and magnetically stirring to obtain a uniform mixed solution. Deoxidizing in vacuum environment for 30min, injecting the mixed solution into a mold composed of a glass plate and a rubber pad, and performing thermal polymerization at 70 ℃ for 8h to obtain SA 3 DMC 25 And (4) gelling.
(3) Preparation of Glycerol/PDA Mixed replacement liquid
DA was dissolved in phosphate buffer (pH = 8.5) to prepare DA (1 wt% to 10 wt%) solutions of different concentrations. PDA chains were generated by stirring at room temperature in an air atmosphere for 30 min. The obtained dispersion liquids with different mass concentrations and glycerol are compounded according to the volume ratio (glycerol: 80v/v%, PDA dispersion liquid: 20 v/v%) to form the mixed replacement liquid.
(4) Preparation of SA 3 DMC 25 Base composite organic gel
At room temperature, SA 3 DMC 25 The gel was immersed in a displacement solution (glycerol: 80v/v%, PDA dispersion: 20 v/v%) having a PDA dispersion concentration of 1wt%. Standing in air atmosphere for 24h to obtain SA 3 DMC 25 The organic gel is compounded.
Example 2
(1) Preparing SA/DMC mixed solution
3.125g of DMC solution (80 wt.%) was mixed well in 6.575g of deionized water. Adding 0.3g of sodium alginate powder into the obtained uniform mixed solution, heating and stirring at 70 ℃ for 5h to obtain a uniform SA/DMC mixed solution, and cooling for later use.
(2) Preparation of SA 3 DMC 25 Gel
Adding initiator potassium peroxodisulfate (0.025 g) and crosslinking agent PEGDA400 (250 μ L,0.1 g/mL) into the SA/DMC mixed solution, performing ultrasonic treatment for 5min, and magnetically stirring to obtain a uniform mixed solution. Deoxidizing in vacuum environment for 30min, injecting the mixed solution into a mold composed of a glass plate and a rubber pad, and performing thermal polymerization at 70 ℃ for 8h to obtain SA 3 DMC 25 And (4) gelling.
(3) Preparation of Glycerol/PDA Mixed replacement fluid
DA was dissolved in phosphate buffer (pH = 8.5) to prepare solutions of DA (1 wt% to 10 wt%) at different concentrations. PDA chains were generated by stirring at room temperature in an air atmosphere for 30 min. The obtained dispersion liquids with different mass concentrations and glycerol are compounded according to the volume ratio (glycerol: 80v/v%, PDA dispersion liquid: 20 v/v%) to form the mixed replacement liquid.
(4) Preparation of SA 3 DMC 25 Composite organic gel
At room temperature, SA 3 DMC 25 The gel was placed in a displacement solution (glycerol: 80v/v%, PDA dispersion: 20 v/v%), wherein the PDA dispersion concentration was 3wt%. Standing in air atmosphere for 24h to obtain SA 3 DMC 25 And (3) compounding the organic gel.
Example 3
(1) Preparing SA/DMC mixed solution
3.125g of DMC solution (80 wt.%) was mixed well in 6.575g of deionized water. Adding 0.3g of sodium alginate powder into the obtained uniform mixed solution, heating and stirring at 70 ℃ for 5h to obtain a uniform SA/DMC mixed solution, and cooling for later use.
(2) Preparation of SA 3 DMC 25 Gel
Adding initiator potassium peroxodisulfate (0.025 g) and crosslinking agent PEGDA400 (250 μ L,0.1 g/mL) into the SA/DMC mixed solution, performing ultrasonic treatment for 5min, and magnetically stirring to obtain a uniform mixed solution. Deoxidizing in vacuum environment for 30min, injecting the mixed solution into a mold composed of a glass plate and a rubber pad, and performing thermal polymerization at 70 ℃ for 8h to obtain SA 3 DMC 25 And (4) gelling.
(3) Preparation of Glycerol/PDA Mixed replacement liquid
DA was dissolved in phosphate buffer (pH = 8.5) to prepare solutions of DA (1 wt% to 10 wt%) at different concentrations. The PDA chains were generated by stirring in an air atmosphere at room temperature for 30 min. The obtained dispersion liquids with different mass concentrations and glycerol are compounded according to the volume ratio (glycerol: 80v/v%, PDA dispersion liquid: 20 v/v%) to form the mixed replacement liquid.
(4) Preparation of SA 3 DMC 25 Composite organic gel
At room temperature, SA 3 DMC 25 The gel was placed in a displacement solution (glycerol: 80v/v%, PDA dispersion: 20 v/v%) having a PDA dispersion concentration of 5wt%. Standing in air atmosphere for 24h to obtain SA 3 DMC 25 The organic gel is compounded.
Example 4
(1) Preparing SA/DMC mixed solution
3.125g of DMC solution (80 wt.%) was mixed well in 6.575g of deionized water. Adding 0.3g of sodium alginate powder into the obtained uniform mixed solution, heating and stirring at 70 ℃ for 5h to obtain a uniform SA/DMC mixed solution, and cooling for later use.
(2) Preparation of SA 3 DMC 25 Gel
Adding initiator potassium peroxodisulfate (0.025 g) and crosslinking agent PEGDA400 (250 μ L,0.1 g/mL) into the SA/DMC mixed solution, performing ultrasonic treatment for 5min, and magnetically stirring to obtain a uniform mixed solution. Deoxidizing in vacuum environment for 30min, injecting the mixed solution into a mold composed of a glass plate and a rubber pad, and performing thermal polymerization at 70 ℃ for 8h to obtain SA 3 DMC 25 And (4) gelling.
(3) Preparation of Glycerol/PDA Mixed replacement liquid
DA was dissolved in phosphate buffer (pH = 8.5) to prepare solutions of DA (1 wt% to 10 wt%) at different concentrations. The PDA chains were generated by stirring in an air atmosphere at room temperature for 30 min. The obtained dispersion liquids with different mass concentrations and glycerol are compounded according to the volume ratio (glycerol: 80v/v%, PDA dispersion liquid: 20 v/v%) to form the mixed replacement liquid.
(4) Preparation of SA 3 DMC 25 Composite organic gel
At room temperature, SA 3 DMC 25 The gel was placed in a displacement solution (glycerol: 80v/v%, PDA dispersion: 20 v/v%), wherein the PDA dispersion concentration was 7wt%. Standing in air atmosphere for 24h to obtain SA 3 DMC 25 The organic gel is compounded.
Example 5
(1) Preparing SA/DMC mixed solution
3.125g of DMC solution (80 wt.%) were mixed homogeneously in 6.575g of deionized water. And adding 0.3g of sodium alginate powder into the obtained uniform mixed solution, heating and stirring at 70 ℃ for 5 hours to obtain a uniform SA/DMC mixed solution, and cooling for later use.
(2) Preparation of SA 3 DMC 25 Gel
Adding initiator potassium peroxodisulfate (0.025 g) and crosslinking agent PEGDA400 (250 μ L,0.1 g/mL) into the SA/DMC mixed solution, performing ultrasonic treatment for 5min, and magnetically stirring to obtain a uniform mixed solution. Deoxidizing in vacuum environment for 30min, injecting the mixed solution into a mold composed of a glass plate and a rubber pad, and performing thermal polymerization at 70 ℃ for 8h to obtain SA 3 DMC 25 And (4) gelling.
(3) Preparation of Glycerol/PDA Mixed replacement fluid
DA was dissolved in phosphate buffer (pH = 8.5) to prepare DA (1 wt% to 10 wt%) solutions of different concentrations. PDA chains were generated by stirring at room temperature in an air atmosphere for 30 min. The obtained dispersion liquid with different mass concentrations and glycerol are compounded according to the volume ratio (glycerol: 80v/v%, PDA dispersion liquid: 20 v/v%) to form a mixed replacement liquid.
(4) Preparation of SA 3 DMC 25 Composite organic gel
At room temperature, SA 3 DMC 25 The gel was placed in a displacement solution (glycerol: 80v/v%, PDA dispersion: 20 v/v%) having a PDA dispersion concentration of 9wt%. Standing in air atmosphere for 24h to obtain SA 3 DMC 25 And (3) compounding the organic gel.
Example 6
(1) Preparing SA/DMC mixed solution
3.125g of DMC solution (80 wt.%) was mixed well in 6.575g of deionized water. And adding 0.3g of sodium alginate powder into the obtained uniform mixed solution, heating and stirring at 70 ℃ for 5 hours to obtain a uniform SA/DMC mixed solution, and cooling for later use.
(2) Preparation of SA 3 DMC 25 Gel
Adding initiator potassium peroxodisulfate (0.025 g) and crosslinking agent PEGDA400 (250 mu L,0.1 g/mL) into the SA/DMC mixed solution, performing ultrasonic treatment for 5min, and performing magnetic stirring until a uniform mixed solution is obtained. Deoxidizing in vacuum for 30min, injecting the mixture into a mold composed of a glass plate and a rubber pad, and performing thermal polymerization at 70 ℃ for 8h to obtain SA 3 DMC 25 And (4) gelling.
(3) Preparation of Glycerol/PDA Mixed replacement fluid
DA was dissolved in phosphate buffer (pH = 8.5) to prepare solutions of DA (1 wt% to 10 wt%) at different concentrations. The PDA chains were generated by stirring in an air atmosphere at room temperature for 30 min. The obtained dispersion liquids with different mass concentrations and glycerol are compounded according to the volume ratio (glycerol: 80v/v%, PDA dispersion liquid: 20 v/v%) to form the mixed replacement liquid.
(4) Preparation of SA 3 DMC 25 Composite organic gel
At room temperature, SA 3 DMC 25 The gel was placed in a displacement solution (glycerol: 80v/v%, PDA dispersion: 20 v/v%) having a PDA dispersion concentration of 10% by weight. Standing in air atmosphere for 24h to obtain SA 3 DMC 25 And (3) compounding the organic gel.
Claims (6)
1. The utility model provides a quaternary ammonium salt light and heat is antibiotic motion colloid dressing in coordination which characterized in that: the quaternary ammonium salt photo-thermal synergistic antibacterial sports colloid dressing comprises sodium alginate, methacryloyloxyethyl trimethyl ammonium chloride, polydopamine and glycerol.
2. The sports gel dressing according to claim 1, wherein the main components are, in mass percent and volume percent, SA: 1-3wt%, DMC:20wt% -30wt%, PDA: 1-10 wt%, glycerol: 80v/v%.
3. The sports gel dressing according to claim 1 or 2, wherein the sports gel dressing has multiple functions of freezing resistance, water retention, photo-thermal response, synergistic antibacterial property and the like.
4. The sports gel dressing according to any one of claims 1 to 3, wherein the preparation method comprises the following steps:
(1) Preparing SA/DMC mixed solution
Uniformly mixing DMC solution (80 wt%) in deionized water, adding sodium alginate powder into the obtained uniformly mixed solution, heating and stirring at 70 ℃ for 5 hours to obtain uniform SA/DMC mixed solution, and cooling for later use;
(2) Preparation of SA/DMC gel
Adding initiator potassium peroxodisulfate and cross-linking agent PEGDA400 into the SA/DMC mixed solution, performing ultrasonic dispersion, magnetically stirring to obtain uniform mixed solution, removing oxygen in a vacuum environment, injecting the mixed solution into a mold consisting of a glass plate and a rubber pad, and performing thermal polymerization at 70 ℃ for 8 hours to obtain SA/DMC gel;
(3) Preparation of Glycerol/PDA Mixed replacement liquid
Dissolving DA in a phosphate buffer solution (pH = 8.5), preparing DA (1 wt% -10 wt%) solutions with different concentrations, stirring for 30min at room temperature in an air atmosphere to generate PDA chains, and compounding the obtained dispersion liquid with different mass concentrations and glycerol according to a volume ratio to form a mixed replacement liquid;
(4) Preparation of SA/DMC-based composite organogel
And (3) placing the gel into different mixed replacement liquids at room temperature, placing for 24h in an air atmosphere, and wiping the surface of the gel to dry to obtain the composite organic gel.
5. The method according to claim 4, wherein the buffer is phosphate buffer and the pH is 8.5.
6. The method for preparing a composite hemostatic sponge according to claim 4, wherein the volume ratio of the mixed replacement liquid is glycerol 80v/v%, the volume ratio of the PDA dispersion liquid is: 20v/v%.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106693042A (en) * | 2016-12-01 | 2017-05-24 | 北京化工大学 | Antibacterial hydrogel dressing and preparation method |
| WO2018045905A1 (en) * | 2016-09-07 | 2018-03-15 | 江南大学 | Catechol modified biological macromolecular scaffold material and preparation method therefor |
| CN108329497A (en) * | 2018-01-19 | 2018-07-27 | 深圳大学 | A kind of freeze proof hydrogel of moisturizing and preparation method thereof |
| CN111905143A (en) * | 2020-06-17 | 2020-11-10 | 西安交通大学 | Multifunctional tissue adhesion crystal gel dressing and preparation method and application thereof |
| CN112641994A (en) * | 2020-12-16 | 2021-04-13 | 北京化工大学常州先进材料研究院 | Sports colloid dressing based on betaine derivative and alginate |
-
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- 2022-07-25 CN CN202210876927.6A patent/CN115227865A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018045905A1 (en) * | 2016-09-07 | 2018-03-15 | 江南大学 | Catechol modified biological macromolecular scaffold material and preparation method therefor |
| CN106693042A (en) * | 2016-12-01 | 2017-05-24 | 北京化工大学 | Antibacterial hydrogel dressing and preparation method |
| CN108329497A (en) * | 2018-01-19 | 2018-07-27 | 深圳大学 | A kind of freeze proof hydrogel of moisturizing and preparation method thereof |
| CN111905143A (en) * | 2020-06-17 | 2020-11-10 | 西安交通大学 | Multifunctional tissue adhesion crystal gel dressing and preparation method and application thereof |
| CN112641994A (en) * | 2020-12-16 | 2021-04-13 | 北京化工大学常州先进材料研究院 | Sports colloid dressing based on betaine derivative and alginate |
Non-Patent Citations (1)
| Title |
|---|
| HUIFENG DONG，ET AL.: "Smart Polycationic Hydrogel Dressing for Dynamic Wound Healing"，Huifeng Dong，et al.，Small，第18卷第25期，第1-14页", SMALL * |
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