CN115227804A - Hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth and preparation method thereof - Google Patents

Abstract

The invention discloses a hair growth liquid for preventing seborrheic alopecia and promoting hair growth and a preparation method thereof, belonging to the technical field of hair growth liquid and comprising the following raw materials in percentage by weight: 1-6% of helichrysum extract, 1-6% of biota orientalis extract, 0.005-0.03% of acetyl tetrapeptide-3,0.01-0.1% of myristoyl pentapeptide-17,0.005-0.025% of biotin 3 peptide-1,0.01-0.02% of fullerene derivative and the like; can promote the proliferation and differentiation of hair follicle cells, reduce the damage of dihydrotestosterone to the health of hair follicles, and provide sufficient nutrients for promoting keratin expression and synthesis. The addition of acetyl tetrapeptide-3 and biotin tripeptide-1 promotes the proliferation and differentiation of hair follicle cells; myristoyl pentapeptide-17 provides nutrients sufficient to promote keratin expression and synthesis, and promotes hair formation and growth.

Description

Hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth and preparation method thereof

Technical Field

The invention belongs to the technical field of daily chemical industry and washing and caring, and particularly relates to a hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth and a preparation method thereof.

Background

Seborrheic Alopecia (SA), also known as androgenetic alopecia, is the most common clinical alopecia disorder. More than 80% of patients with alopecia are investigated as seborrheic alopecia or alopecia accompanied therewith. Epidemiological survey shows that the traditional Chinese medicine composition is increased year by year and tends to be younger, so that the traditional Chinese medicine composition is not only harmful to the appearance and the beauty, but also brings great mental stress and psychological burden to patients and seriously influences the life quality of the patients.

The clinical manifestations of male seborrheic alopecia are that the hair lines of forehead, especially two temples gradually move upwards, the hair on the top of the head is sparse, the hair on the forehead and the top of the head gradually falls off, and the scalp can be accompanied with greasy skin, desquamation and pruritus, female seborrheic alopecia is mainly diffuse alopecia, the hair in the central area of the scalp is progressively reduced and thinned, and the forehead hair line has no obvious change. The disease is significantly associated with genetic factors and abnormal metabolism of scalp androgens, and patients with family history progress faster and often tend to develop early in age.

Seborrheic alopecia is mainly caused by androgen and receptor abnormality, and is also related to genetic, psychological and other factors; modern medicine considers that SA is an autosomal dominant polygenic hereditary alopecia with androgen dependence, excessive androgen or increased sensitivity to androgen is a main factor of the occurrence of the disease, the occurrence of SA is directly related to the increased levels of testosterone (T) and a metabolite, namely Dihydrotestosterone (DHT) and an Androgen Receptor (AR), 5 alpha-reductase (5 alpha-R) plays an important role, and long-term mental imbalance, mental stress, eating disorder and germ infection are important factors for inducing or aggravating the disease.

The inventor considers that the system solves the seborrheic alopecia and needs to coordinate three aspects simultaneously: the hair follicle cell proliferation and differentiation are promoted, the damage of Dihydrotestosterone (DHT) to the hair follicle health is reduced, and nutrients for promoting keratin expression and synthesis are provided. The prior approved medicines only aim at influencing factors on one aspect, and the existing hair growth nutrient solution products have the problems of unclear component targets and weak pertinence.

At present, the main medical means for treating seborrheic alopecia are external minoxidil and oral 5 alpha-reductase (5 alpha-R) inhibitor androstamines, such as finasteride and dutasteride, which are mainly finasteride.

Minoxidil is a non-specific drug for treating alopecia, commonly used concentrations are 20 and 50mg/mL, and is the only FDA approved topical drug for treating seborrheic alopecia. The mechanism for preventing and treating alopecia is as follows: opening potassium ion channel, inhibiting calcium ion inflow, reducing the inhibition of epidermal growth factor on hair growth, promoting the proliferation and differentiation of hair follicle epithelial cells, up-regulating vascular endothelial cell growth factor, improving local blood supply, etc. But the use process may cause hirsutism on the face and limbs, and the treatment effect gradually disappears after the use is stopped. Scalp itch and dandruff adverse reactions occur on some users, and the condition that hair grown by the users is fine and cannot be thickened and grown continuously also exists.

At present, the market is full of a plurality of synthetic steroidal and non-steroidal 5 alpha-R inhibitors, however, the synthetic inhibitors have a plurality of adverse reactions and are difficult to meet the requirements of patients. Finasteride, a steroid selective inhibitor specific for type ii 5 α -reductase (5 α -R), reduces the conversion of testosterone (T) to Dihydrotestosterone (DHT), which is more strongly bound to the receptor, thereby reducing the effects of androgens on hair loss. Finasteride can continuously improve the growth condition of hair, but has adverse reactions such as sexual dysfunction, spermatozoa reduction, gynecomastia and the like.

Besides drug treatment, some hair restorers containing composite plant components and polypeptide components are also available on the market. The functions of the plant components of the hair restorer are described as promoting blood circulation rather than the targeting effect of specific substances, and the hair restorer has the defects of unclear component action targets, unclear plant component synergistic action mechanism, slow effect taking, weak target component pertinence in production and extraction technology and inaccurate quantification.

The 5 alpha-R inhibitor in the plant has wide development prospect, and various plant extracts have been reported to have stronger 5 alpha-R inhibition activity at home and abroad in two years. Therefore, the 5 alpha-R inhibitor with clear effect and remarkable activity is screened from the plants, is used for a new generation of seborrheic alopecia health product with clear drug effect substance basis, and brings good news to seborrheic alopecia patients at home and abroad.

Disclosure of Invention

In order to solve the technical problems, the invention provides a hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth and a preparation method thereof.

The purpose of the invention can be realized by the following technical scheme:

a hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth comprises the following raw materials in percentage by weight: 0.005-0.03% of acetyl tetrapeptide-3,0.01-0.1% of myristoyl pentapeptide-17,0.005-0.025% of biotin 3 peptide-1,1-6% of helichrysum extracting solution, 1-6% of biota orientalis extracting solution, 0.2-2% of estrogen Chinese medicine extracting solution, 0.2-2% of panthenol, 0.2-0.5% of phenoxyethanol, 0.5-3% of cornflower extract, 0.01-0.02% of fullerene derivative and the balance of alcohol-water buffer solution.

Acetyl tetrapeptide-3:

the size of the hair follicle is determined by the size of the hair papilla and the extracellular matrix. Healthy hair papillae produce extracellular matrix proteins such as collagen iii and fixed fibers such as laminin collagen vii, which make the hair root strong. If the extracellular matrix becomes renewed, the hair becomes brittle. This cycle continues and the follicle eventually collapses. Acetyl tetrapeptide-3 accelerates the synthesis of extracellular matrix proteins such as laminin, collagen III and collagen VII by synthesizing fibroblasts, and directly acts on perifollicular tissues to increase the volume and length of hair follicles. Repairing the connective tissue of epidermis and dermis to promote the hair to be fixed in hair follicle.

Myristoyl pentapeptide-17:

the control of the hair growth cycle is associated with a complex interaction between the two tissues of the hair bulb and the hair papilla, which is promoted by the nerve contained in the hair papilla and the blood supply providing nutrition to the hair. Myristoyl pentapeptide-17 is a pentapeptide effective in promoting hair growth, and directly acts on keratin gene to promote keratin production.

Biotin 3 peptide-1:

is a tripeptide combining vitamin H and a Matrikine series signal peptide GHK. The biotin 3 peptide-1 can increase the synthesis of extracellular matrix such as collagen IV and laminin 5, delay the aging of hair follicle, improve the hair follicle structure, and is beneficial to fixing hair in the dermis hair follicle to prevent alopecia. Activating tissue repair gene expression, and is favorable for skin structure reconstruction and repair. Promoting cell proliferation and differentiation, and stimulating hair growth.

And (3) helichrysum extract:

also called permanent flower, originally produced in Europe, western Asia and North America, is also planted in Xinjiang and Shandong in China, and is a herbal medicinal plant of Compositae. The extract contains active ingredients of Helichrysum total flavone with high total flavone content. The substance has strong inhibition on the activity of 5 alpha-R reductase in an in vitro test of 5 alpha-R reductase. Further inhibit 5 alpha-R reduction reaction, make testosterone (T) not be reduced into Dihydrotestosterone (DHT), reduce inhibition and poison of androgen to hair follicle.

Cacumen biotae extract:

is evergreen arbor of Cupressaceae, and is a special medicinal plant of China. The extract contains thuja flavone and quercetin as effective components. The substance has strong inhibition on the activity of 5 alpha-R reductase in an in vitro test of 5 alpha-R reductase. Further inhibit 5 alpha-R reduction reaction, make testosterone (T) not be reduced into Dihydrotestosterone (DHT), and reduce inhibition and toxicity of androgen on hair follicle.

And (3) sophora flavescens extract:

the effective component of the extract is kuh-seng flavone. It has effects in improving capillary microcirculation, and has estrogen-like effect (androgen antagonism) and certain 5 α -R reductase inhibitory effect.

Astragalus extract:

the extract contains effective components of astragaloside, calycosin, and astragaloside. Improve capillary microcirculation, and has estrogen-like effect (androgen antagonism) and certain 5 alpha-R reductase inhibition.

And (3) glossy privet fruit extract:

the extract contains fructus Ligustri Lucidi flavone and oleanolic acid as effective components. Improve capillary microcirculation, and has estrogen-like effect (androgen antagonism) and certain 5 alpha-R reductase inhibition.

Panthenol:

also known as provitamin B5, permeates the moisturizer, moisturizes the stratum corneum, opens skin pathways, and helps absorb other active ingredients. Stimulating epidermal cell growth, inhibiting inflammation, and protecting mucosa. Providing hair growth nutrition.

Further, the fullerene derivative is prepared by the following steps:

step S1, adding carbon 60 into a three-neck flask, slowly dropwise adding liquid bromine, magnetically stirring at room temperature for 72-96h, decompressing and filtering after the reaction is finished, absorbing extracted bromine steam with anhydrous sodium sulfite solution, standing a filter cake at room temperature for 24h, and then drying in vacuum at 60 ℃ for 10h to prepare modified fullerene, wherein the dosage ratio of the carbon 60 to the liquid bromine is controlled to be 400-500 mg: 100mL;

and S2, adding the modified fullerene into absolute ethyl alcohol, magnetically stirring for 30min, dropwise adding a sodium hydroxide aqueous solution with the mass fraction of 13%, uniformly stirring at room temperature and reacting for 6h, dropwise adding concentrated hydrochloric acid to adjust the pH until the pH is =5-5.6 after the reaction is finished, centrifuging, collecting a supernatant, carrying out rotary evaporation on the supernatant at 45 ℃, extracting a rotary evaporation product with acetone, repeating the steps for three times to obtain a crude product, purifying to obtain a fullerene derivative, and controlling the modified fullerene, the absolute ethyl alcohol and the sodium hydroxide aqueous solution to be 200-250 mg: 25-30 mL: 40mL.

Modifying carbon 60 by liquid bromine in step S1 to prepare modified fullerene which is bromo-fullerene, and then preparing fullerene derivative which is amphiphilic fullerene derivative with hydroxyl and ethoxy by taking bromo-fullerene, ethanol and sodium hydroxide as raw materials in step S2, wherein on one hand, the fullerene derivative can be used as a medicine, and the amphiphilic fullerene derivative can smoothly pass through hydrophilic and lipophilic regions of a cell membrane and has good compatibility with organisms; on the other hand, the solubility of oil soluble medicines such as trace essential oil in the cacumen Platycladi extract in organism is increased, and the effect of the medicine is effectively improved.

Further, the helichrysum extract, the biota orientalis extract, the estrogen Chinese medicine extract and the cornflower extract are prepared by the following steps:

respectively placing Helichrysum Chinense L, folium Platycladi, estrogen Chinese medicinal material and cornflower in oven, drying at 40-45 deg.C for 2 hr, adding 50% ethanol water solution as extraction solvent at a ratio of 1: 10 into a sealed container, soaking at 60 deg.C for 2 hr, maintaining temperature and performing ultrasonic treatment at 40Hz for 2 hr, and filtering to obtain Helichrysum Chinense L extractive solution, folium Platycladi extractive solution, estrogen Chinese medicinal material extractive solution and cornflower extract.

Furthermore, the estrogen Chinese herbal medicine is prepared by mixing any one or more of astragalus, radix sophorae flavescentis and glossy privet fruit according to any proportion.

Further, the alcohol-water buffer solution is prepared by mixing 1,3-propylene glycol and deionized water according to the weight ratio of 1: 50-60.

A preparation method of a hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth comprises the following steps:

mixing acetyl tetrapeptide-3, myristoyl pentapeptide-17, biotin 3 peptide-1, helichrysum extract, folium Platycladi extract, estrogen Chinese medicinal extract, panthenol, phenoxyethanol, cornflower extract and fullerene derivative, vortex and shaking for 15-30min, and adding alcohol-water buffer solution to obtain hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth.

The invention has the beneficial effects that:

the hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth prepared by the invention can promote the proliferation and differentiation of hair follicle cells, reduce the damage of Dihydrotestosterone (DHT) to the health of hair follicles and provide sufficient nutrients for promoting keratin expression and synthesis. The addition of acetyl tetrapeptide-3 and biotin tripeptide-1 promotes the proliferation and differentiation of hair follicle cells; the helichrysum extract and the biota orientalis extract inhibit 5 alpha-R reduction reaction, reduce the damage of Dihydrotestosterone (DHT) to the health of hair follicles and eliminate the inhibition of hair follicle growth; myristoyl pentapeptide-17 provides nutrients sufficient to promote keratin expression and synthesis, and promotes hair formation and growth.

The helichrysum extract and the biota orientalis extract can effectively inhibit 5 alpha-R reduction reaction, reduce the damage of Dihydrotestosterone (DHT) to the health of hair follicles and eliminate the inhibition to the growth of the hair follicles. There is no adverse effect of androgen medicine on body.

The polypeptide is added into the formula of the invention, and the invention has the following effects:

a. strong activity: high activity and low dosage.

b. The effect is mild and definite: unlike hormones and injectable products, the important feature of polypeptides is that they are mild but definite in action.

c. High safety: because of the homology of the polypeptide and organism substances, the high compatibility of the polypeptide when acting on skin is ensured, and the potential safety hazard caused by injection is avoided.

d. High absorbency: compared with collagen, the polypeptide has smaller molecular weight and can penetrate into skin to play a role.

e. Quality controllability: each applied active polypeptide is a single substance, the quality of the active polypeptide is easier to control compared with a plant extract, and the purity of the active polypeptide can reach more than 98%.

The solvent is an alcohol-water buffer solution, the solvent has good compatibility with all components, is not greasy, and can be matched with the minoxidil tincture for use, and the hair growth solution prepared by the method is smeared on the root and scalp of the hair, is used for 2-5mL each time, is used for 1-2 times every day, and is absorbed by massage.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The fullerene derivative is prepared by the following steps:

step S1, adding carbon 60 into a three-neck flask, slowly dropwise adding liquid bromine, magnetically stirring for 72 hours at room temperature, decompressing and filtering after the reaction is finished, absorbing extracted bromine steam with anhydrous sodium sulfite solution, standing a filter cake for 24 hours at room temperature, and then drying in vacuum for 10 hours at 60 ℃ to prepare modified fullerene, wherein the dosage ratio of the carbon 60 to the liquid bromine is controlled to be 400 mg: 100mL;

and S2, adding the modified fullerene into absolute ethyl alcohol, magnetically stirring for 30min, dropwise adding a sodium hydroxide aqueous solution with the mass fraction of 13%, uniformly stirring at room temperature and reacting for 6h, dropwise adding concentrated hydrochloric acid to adjust the pH until the pH is =5 after the reaction is finished, centrifuging, collecting a supernatant, carrying out rotary evaporation on the supernatant at 45 ℃, extracting a rotary evaporation product with acetone, repeating the steps for three times to obtain a crude product, purifying to obtain a fullerene derivative, and controlling the modified fullerene, the absolute ethyl alcohol and the sodium hydroxide aqueous solution to be 200 mg: 25 mL: 40mL.

Example 2

The fullerene derivative is prepared by the following steps:

step S1, adding carbon 60 into a three-neck flask, slowly dropwise adding liquid bromine, magnetically stirring for 80 hours at room temperature, decompressing and filtering after the reaction is finished, absorbing extracted bromine steam with anhydrous sodium sulfite solution, standing a filter cake for 24 hours at room temperature, and then drying in vacuum for 10 hours at 60 ℃ to prepare modified fullerene, wherein the dosage ratio of the carbon 60 to the liquid bromine is controlled to be 450 mg: 100mL;

and S2, adding the modified fullerene into absolute ethyl alcohol, magnetically stirring for 30min, dropwise adding a sodium hydroxide aqueous solution with the mass fraction of 13%, uniformly stirring at room temperature and reacting for 6h, dropwise adding concentrated hydrochloric acid to adjust the pH until the pH is =5.2 after the reaction is finished, centrifuging, collecting a supernatant, carrying out rotary evaporation on the supernatant at 45 ℃, extracting a rotary evaporation product with acetone, repeating the steps for three times to obtain a crude product, purifying to obtain a fullerene derivative, and controlling the modified fullerene, the absolute ethyl alcohol and the sodium hydroxide aqueous solution to be 220 mg: 28 mL: 40mL.

Example 3

The fullerene derivative is prepared by the following steps:

step S1, adding carbon 60 into a three-neck flask, slowly dropwise adding liquid bromine, magnetically stirring for 96 hours at room temperature, decompressing and filtering after reaction is finished, absorbing extracted bromine steam with anhydrous sodium sulfite solution, standing a filter cake for 24 hours at room temperature, and then drying in vacuum for 10 hours at 60 ℃ to prepare modified fullerene, wherein the dosage ratio of the carbon 60 to the liquid bromine is controlled to be 500 mg: 100mL;

and S2, adding the modified fullerene into absolute ethyl alcohol, magnetically stirring for 30min, dropwise adding a sodium hydroxide aqueous solution with the mass fraction of 13%, uniformly stirring at room temperature and reacting for 6h, dropwise adding concentrated hydrochloric acid to adjust the pH until the pH is =5.6 after the reaction is finished, centrifuging, collecting a supernatant, carrying out rotary evaporation on the supernatant at 45 ℃, extracting a rotary evaporation product with acetone, repeating the steps for three times to obtain a crude product, purifying to obtain a fullerene derivative, and controlling the modified fullerene, the absolute ethyl alcohol and the sodium hydroxide aqueous solution to be 250 mg: 30 mL: 40mL.

Example 4

A hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth comprises the following raw materials in percentage by weight: 0.005% acetyl tetrapeptide-3,0.01% myristoyl pentapeptide-17,0.005% biotin 3 peptide-1,1% Helichrysum arborvitae extract, 1% cacumen Platycladi extract, 0.2% radix astragali extract, 0.2% panthenol, 0.2% phenoxyethanol, 0.5% cornflower extract, 0.01% fullerene derivative, and the balance alcohol-water buffer solution.

The hair growth liquid comprises the following steps:

mixing acetyl tetrapeptide-3, myristoyl pentapeptide-17, biotin 3 peptide-1, helichrysum extract, folium Platycladi extract, radix astragali extract, panthenol, phenoxyethanol, cornflower extract and fullerene derivative, vortex and shake for 15min, and adding alcohol-water buffer solution to obtain hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth.

Example 5

A hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth comprises the following raw materials in percentage by weight: 0.01% of acetyl tetrapeptide-3,0.05% of myristoyl pentapeptide-17,0.015% of biotin 3 peptide-1,3% of helichrysum extract, 3% of cacumen biotae extract, 1% of glossy privet fruit extract, 1% of panthenol, 0.3% of phenoxyethanol, 1.5% of cornflower extract, 0.01% of fullerene derivative and the balance of an alcohol-water buffer solution.

The hair growth liquid comprises the following steps:

mixing acetyl tetrapeptide-3, myristoyl pentapeptide-17, biotin 3 peptide-1, helichrysum italicum extract, folium Platycladi extract, fructus Ligustri Lucidi extract, panthenol, phenoxyethanol, cornflower extract and fullerene derivative, vortex and shake for 20min, and adding alcohol water buffer solution to obtain hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth.

Example 6

A hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth comprises the following raw materials in percentage by weight: 0.03% of acetyl tetrapeptide-3,0.1% of myristoyl pentapeptide-17,0.025% of biotin 3 peptide-1,6% of helichrysum extract, 6% of cacumen biotae extract, 2% of radix sophorae flavescentis extract, 2% of panthenol, 0.5% of phenoxyethanol, 3% of cornflower extract, 0.02% of fullerene derivative and the balance of an alcohol-water buffer solution.

The hair growth liquid comprises the following steps:

mixing acetyl tetrapeptide-3, myristoyl pentapeptide-17, biotin 3 peptide-1, helichrysum extract, folium Platycladi extract, radix Sophorae Flavescentis extract, panthenol, phenoxyethanol, cornflower extract and fullerene derivative, vortex and shake for 30min, and adding alcohol-water buffer to obtain hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth.

Comparative example 1

This comparative example compares to example 4 without the addition of fullerene derivatives.

Comparative example 2

In comparison with example 4, the formulation of this comparative example was adjusted to: 1% of helichrysum extract, 1% of cacumen biotae extract, 0.2% of astragalus extract, 0.2% of panthenol, 0.2% of phenoxyethanol, 0.5% of cornflower extract, 0.01% of fullerene derivative and the balance of alcohol-water buffer solution.

Comparative example 3

This comparative example is a commercially available topical minoxidil hair restorer.

Comparative example 4

The comparative example is a finasteride tablet, a 5mgx 10 tablet/box, prepared as a 2% solution with 75% ethanol by volume fraction prior to the experiment.

Comparative example 5

Compared with example 4, the formula of the comparative example does not contain the helichrysum extracting solution.

Comparative example 6

In comparison with example 4, the formulation of this comparative example does not include cacumen Platycladi extract.

Comparative example 7

Compared with the example 4, the formulation of the comparative example does not contain the astragalus root extract.

The hair growth liquids prepared in examples 4 to 6 and comparative examples 1 to 3 were subjected to animal experiments using C57BL/6 mice to evaluate the efficacy of hair growth, and the results are shown in Table 1 below;

the experimental process comprises the following steps: 110C 57BL/6 mice were used, and the mice were numbered for 6 weeks in each male and female half, and then randomly arranged into example 1, example 2, example 3, example 4, example 5, example 6, comparative example 1, comparative example 2, comparative example 3, comparative example 4 and minoxidil groups, each of which was 10 mice. After being anaesthetized by ether, the mixture of rosin and paraffin (1: 1) is melted by heat, and then the mixture is smeared on the back of a mouse, and is removed after solidification and hardening, and the back of the mouse is smooth and has no wound and hairy roots. And respectively applying corresponding hair growth promoting liquid to the depilated area twice a day (2 mL/piece) the next day after depilation for 30 days. During the period, the condition that the hair grows on the back of the mouse and the condition that the hair grows on the part which is not smeared with the sample are observed, the condition that the hair grows on the part which is smeared with the sample and the condition that the hair grows on the part which is not smeared with the sample are observed by using an image analyzer each time, the ratio of the two is compared, the condition of each mouse is calculated respectively, and the average value is counted.

TABLE 1

As can be seen from table 1 above, the hair restorer prepared in examples 4-6 has significant efficacy, and the various active ingredients in the hair restorer have synergistic effect in promoting hair growth.

Clinical trial

The hair restorer prepared in examples 4-6 and comparative examples 1-3 was clinically tested for the effect of promoting hair growth by selecting potential hair restorers according to the results of animal experiments to perform clinical trials in which 125 persons aged 20-55 years and having alopecia areata, alopecia totalis or baldness, among which 63 persons in males and 62 persons in females, were clinically tested. The test personnel are randomly divided into 5 groups, each group comprises 25 people, the hair growth liquid of the embodiment 4-6 and the minoxidil are respectively used, and the using method comprises the steps of taking about 2-5mL of the hair growth liquid, applying the hair growth liquid to a hair loss part 1-2 times a day, massaging and absorbing the hair loss part once a day in the morning and evening, and continuously using the hair growth liquid for two months. The evaluation criteria of the curative effect are as follows: 1) The effect is shown: stopping alopecia, and growing black hair again in hairless areas; 2) The method has the following advantages: the alopecia is obviously improved, and black hair grows again in partial hairless areas; 3) Micro-effect: hair loss is improved, and vellus hairs grow on bald areas; 4) And (4) invalidation: hair loss was not improved or reduced and no new hair was grown in the hairless areas. The results are shown in table 2 below:

TABLE 2

	Show effect	Is effective	Micro-effect	Nullification	Significant efficiency%
						Example 4	18	2	3	1	75
Example 5	20	3	1	1	80
						Example 6	22	1	1	1	88
Comparative example 1	15	4	3	3	60
						Comparative example 2	8	8	5	4	32
Comparative example 3	16	5	3	1	64

From the above table 2, it can be seen that the hair growth solution prepared by the invention has significant effect and high practicability in clinical application.

The hair growth of C57BL6 mice was examined with the hair restorer prepared in example 5 and comparative examples 4 to 7, and the results are shown in Table 3 below:

laboratory animal

7 weeks old C57BL6BL6 mice, male, SPF grade, 15-20g, 70. The temperature was 22-25 ℃, normal light, and animals were fed standard pelleted mouse chow.

(1) Experiment grouping

70 mice were acclimatized in an air-conditioned room for 1 week with free access to water for feeding, naturally illuminated, and then randomly divided into normal groups (coated with 75% by volume of ethanol), model groups (coated with 100 μ L of 0.05% testosterone solution), example 5, comparative 4, comparative 5, comparative 6, comparative 7, and 6 groups, each group consisting of 10 mice.

(2) Establishment of animal model

The mice are anesthetized with ether 1 day before the experiment, heated and melted by a rosin/paraffin mixture (1:1), then smeared on the back of a C57BL6 mouse in an area of about 2cmX2.5 cm, and removed after solidification and hardening, and the hairs of the mice are induced to enter the growth phase from the resting phase.

(3) Drug treatment

On day 2 of depilation, 100. Mu.L of a 0.05% testosterone solution (0.05 g testosterone dissolved in 100mL 75% ethanol) was applied to the depilated area of the back of each group of mice except the normal group 1 time a day for 28 consecutive days, and after 30min of testosterone solution treatment, 100. Mu.L of a hair restorer solution was applied to the depilated area 1 time a day for 28 consecutive days in the same manner.

After 7-week-old mice had depilated, the skin appeared pink, and the hair entered the resting stage, and the time for the skin color of the mice to change from pink to black and the time for hair to grow were recorded by daily observation.

TABLE 3

Table alignment of model groups to below Normal groups

It can be seen from the above table that the examples of the present invention have a shorter skin discoloration time and a greatly shortened hair emergence time in mice, and can effectively control the adverse effects of testosterone on hair follicles, compared to the normal group and the finasteride control group (comparative example 4).

The foregoing is illustrative and explanatory only of the present invention, and it is intended that the present invention cover modifications, additions, or substitutions by those skilled in the art, without departing from the spirit of the invention or exceeding the scope of the claims.

Claims (6)

1. A hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth is characterized by comprising the following raw materials in percentage by weight: 0.005-0.03% of acetyl tetrapeptide-3,0.01-0.1% of myristoyl pentapeptide-17,0.005-0.025% of biotin 3 peptide-1,1-6% of helichrysum extracting solution, 1-6% of biota orientalis extracting solution, 0.2-2% of estrogen Chinese medicine extracting solution, 0.2-2% of panthenol, 0.2-0.5% of phenoxyethanol, 0.5-3% of cornflower extract, 0.01-0.02% of fullerene derivative and the balance of alcohol-water buffer solution.

2. The hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth according to claim 1, wherein the fullerene derivative is prepared by the following steps:

step S1, adding carbon 60 into a three-neck flask, slowly dropwise adding liquid bromine, magnetically stirring at room temperature for 72-96h, carrying out vacuum filtration after the reaction is finished, standing a filter cake at room temperature for 24h, and carrying out vacuum drying at 60 ℃ for 10h to obtain modified fullerene, wherein the dosage ratio of the carbon 60 to the liquid bromine is controlled to be 400-500 mg: 100mL;

and S2, adding the modified fullerene into absolute ethyl alcohol, magnetically stirring for 30min, dropwise adding a sodium hydroxide aqueous solution with the mass fraction of 13%, uniformly stirring at room temperature and reacting for 6h, dropwise adding concentrated hydrochloric acid to adjust the pH until the pH is =5-5.6 after the reaction is finished, centrifuging, collecting a supernatant, carrying out rotary evaporation on the supernatant at 45 ℃, extracting a rotary evaporation product by using acetone, repeating the steps for three times to obtain a crude product, purifying to obtain the fullerene derivative, and controlling the modified fullerene, the absolute ethyl alcohol and the sodium hydroxide aqueous solution to be 200-250 mg: 25-30 mL: 40mL.

3. The hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth as claimed in claim 1, wherein the helichrysum extract, the biota orientalis extract, the estrogen Chinese medicine extract and the cornflower extract are prepared by the following steps:

respectively placing Helichrysum, folium Platycladi, estrogen Chinese medicinal materials and cornflower in oven, drying at 40-45 deg.C for 2 hr, adding 50% ethanol water solution as extraction solvent at a ratio of 1: 10 into sealed container, soaking at 60 deg.C for 2 hr, maintaining temperature, performing ultrasound treatment at 40Hz for 2 hr, and filtering to obtain Helichrysum extract, folium Platycladi extract, estrogen Chinese medicinal material extract and cornflower extract.

4. The hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth as claimed in claim 3, wherein the estrogen Chinese herbal medicine is one or more of radix astragali, radix sophorae flavescentis and fructus ligustri lucidi mixed in any proportion.

5. The hair tonic for preventing seborrheic alopecia and promoting hair growth according to claim 1, wherein the alcohol-water buffer solution is 1,3-propylene glycol and deionized water mixed in a weight ratio of 1: 50-60.

6. The method for preparing the hair tonic for promoting hair growth for preventing seborrheic alopecia according to claim 1, comprising the steps of:

mixing acetyl tetrapeptide-3, myristoyl pentapeptide-17, biotin 3 peptide-1, helichrysum extract, folium Platycladi extract, estrogen Chinese medicinal extract, panthenol, phenoxyethanol, cornflower extract and fullerene derivative, vortex and shaking for 15-30min, and adding alcohol-water buffer solution to obtain hair growth promoting liquid for preventing seborrheic alopecia and promoting hair growth.