CN115219442A - Full-quantitative urine detection and analysis system and analysis method thereof - Google Patents
Full-quantitative urine detection and analysis system and analysis method thereof Download PDFInfo
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Abstract
The application discloses a full-quantitative urine detection and analysis system and an analysis method thereof, wherein the full-quantitative urine detection and analysis system comprises a full-automatic urine quantitative detector, a data analysis device and a full-quantitative urine clinical detection reagent which is matched with the full-automatic urine quantitative detector and the data analysis device; the full-automatic quantitative urine detector comprises a sample loading module, a sample separate injection module, a reagent loading module, a reagent separate injection module, a reaction cup loading module, a mixing and stirring module, a reaction cup cleaning module and an optical module; the data analysis device comprises a computer module and a printer module; the urine full-quantitative clinical detection reagent used in a matched manner comprises a urine hormone series full-quantitative detection reagent, a urine special protein series full-quantitative detection reagent, a urine biochemical series full-quantitative detection reagent, a urine therapeutic drug monitoring series full-quantitative detection reagent, a urine drug series full-quantitative detection reagent and a urine conventional project series full-quantitative detection reagent. The application also discloses a urine full-quantitative detection and analysis method based on the urine full-quantitative detection and analysis system. The urine full-quantitative detection and analysis system and the analysis method thereof can make timely and accurate judgment on the health condition of a human body and provide a basis for diagnosis and treatment of diseases.
Description
Technical Field
The invention belongs to the technical field of biomedical detection, and particularly relates to a full-quantitative urine detection and analysis system and an analysis method thereof.
Background
Urine testing (Urinalisy) refers to a testing technique for testing and analyzing urine samples by using the principles of physiology, biochemistry, immunology, molecular biology and microorganism and by means of medical microscopes and other medical instruments (such as various urine component analyzers, urinary sediment analyzers, urine cell analyzers, etc.). Clinical detection of urine samples can achieve the purposes of diagnosing, observing curative effects, judging prognosis and the like of major diseases of multiple organs such as a urinary system, a reproductive system, a circulatory system, an endocrine system, a nervous system, liver, gallbladder, pancreas, spleen and the like. Clinical urine detection can help clinicians to intuitively know the changes of physiological functions and pathological conditions of the urinary organs of patients and indirectly reflect the functional conditions of multiple organs and multiple systems of the whole body. Clinical urine examination at home and abroad is very common, and the examination in many fields related to renal function, cardiovascular diseases, metabolic diseases, infectious diseases and the like needs to be carried out by using urine. More conventional urine tests include: such as Glucose (GLU), bilirubin (BIL), urobilinogen (URO), ketone bodies (KET), nitrite (NIT), protein (PRO), pH (pH), occult Blood (BLD), urine Specific Gravity (SG) and White Blood Cells (WBC), casts, pathogens, etc. In addition to these routine tests, urine samples are also frequently used for testing creatinine, hormones and their metabolites, and drugs, etc. The hormone is a series of small molecule or macromolecule signal substances secreted by the endocrine system of a human body, has important significance on various physiological activities of the human body, such as growth, development, metabolism and the like, and a plurality of diseases are related to the secretion and metabolism level of the hormone. Such as vanillylmandelic acid (VMA), a metabolite of catecholamine type hormones, becomes one of the gold standards for early screening of pediatric neuroblastoma and early diagnosis of pheochromocytoma, which are recognized by medical circles at home and abroad.
However, the current urine inspection technology in China has a larger gap compared with the international leading level, and the qualitative inspection method of dry chemical test paper is mainly adopted to detect the urine components in China, and the microscopic inspection method and the urine sediment analyzer are adopted to detect the visible components in urine. The dry chemical qualitative detection method uses dry chemical test paper strips, which can only provide qualitative analysis results, are influenced by a plurality of factors and are easy to generate false negative and false positive results. Microscopy is generally used as a gold standard for detecting urine visible components, but has insurmountable defects, such as long detection time, loss, damage and deformation of erythrocytes during centrifugation, human errors among different operators, and incapability of detecting lysed erythrocytes, which may cause false negative results in diagnosis. The principle of the urinary sediment analyzer is to detect and analyze the visible components such as red blood cells, white blood cells, casts, crystals, bacteria, epithelial cells and the like in urine by using the principle of flow cytometry fluorescence scattering intensity and electrical impedance change, but the urinary sediment analyzer cannot provide the detection result of the urine body components.
At present, most of the current urine detection processes in China firstly utilize a urine dry chemical test paper method to detect and analyze urine body components, and then utilize a urine sediment analyzer to detect urine visible components after the urine detection processes are finished, so that not only can a full quantitative result be provided, but also integrated and intelligent operation cannot be realized, time and labor are wasted, and a great deal of inconvenience is brought to clinical diagnosis. Along with the continuous improvement of the medical conditions in China, clinicians urgently need to provide more accurate urine full-quantitative inspection information for inspection departments, so that more accurate diagnosis and more deep analysis of the state of an illness are performed on patients, more reasonable treatment means can be adopted by the clinicians, the medicine is taken according to the actual conditions of the patients, the treatment effect of the illness is improved, the medical cost can be saved, and the burden of the patients is relieved.
The CLINITEK AUWi dry reagent urine analysis system manufactured by Siemens (Siemens) is currently one of the most internationally advanced urine test systems, combining an advanced urine cell analyzer and a dry chemical reagent analysis system. Although commonly used abroad, this system still has deficiencies and provides only semi-quantitative results. Foreign Quest Diagnostics companies have partial urine body reagent detection systems, but the project development is not comprehensive, and the liquid reagent development difficulty of individual urine detection projects is large, so that the liquid reagent detection results of partial urine can be provided; at present, no full-quantitative intelligent detection and analysis system capable of providing a relatively comprehensive urine body composition detection result exists internationally. Therefore, the development of an intelligent analysis system capable of providing a full quantitative detection result of urine is an urgent need of the first line of clinic, is a development trend in the field of clinical urine examination in the world, and has high clinical application value and wide market prospect.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a full-quantitative urine detection and analysis system and an analysis method thereof. The urine full-quantitative detection and analysis system can make timely and accurate judgment on the health condition of a human body by performing full-quantitative detection and analysis on a urine sample, and provides a basis for diagnosis and treatment of diseases.
The technical scheme adopted by the invention for solving the technical problem is as follows: the invention provides a fully quantitative urine detection and analysis system, which comprises a fully automatic quantitative urine detector, a data analysis device and a fully quantitative urine clinical detection reagent used in a matched manner.
As an optional scheme, the full-automatic quantitative urine detector comprises a sample loading module, a sample dispensing module, a reagent loading module, a reagent dispensing module, a reaction cup loading module, a mixing and stirring module, a reaction cup cleaning module and an optical module; the data analysis device comprises a computer module and a printer module.
The sample loading module is used for placing a sample cup or a test tube, and conveying the sample cup or the test tube to a position where a sample is sucked by the sample needle through automatic rotation.
The sample dispensing module is provided with a sample needle, the sample needle is provided with a liquid level sensor and is used for sucking a sample with a specified volume from a sample cup or a test tube, the sample dispensing module moves the sample needle to the position of the reaction cup and fills the sample into the reaction cup, and after the sample is filled, the sample needle automatically returns to the cleaning tank to clean the inner wall and the outer wall of the cleaning tank.
The reagent loading module is used for placing a reagent, a diluent and a cleaning solution, and conveying the reagent, the diluent and the cleaning solution to a reagent needle sample sucking position through automatic rotation; the reagent loading module may also be used to store reagents cryogenically, with the reagents in the reagent loading module being in a refrigerated state when power is turned on.
The reagent dispensing module is provided with two reagent needles, the reagent needles are provided with liquid level sensors and used for sucking a reagent with a specified volume from a reagent bottle, the reagent dispensing module moves the reagent needles to the position of the reaction cup and fills the reagent into the reaction cup, and after the reagent filling is finished, the reagent needles automatically return to the cleaning tank to clean the inner wall and the outer wall of the cleaning tank; when the instrument is in operation, the reagent needle for the first reagent fill is on the opposite side when the reagent needle for the second reagent fill is in the wash position.
The reaction cup loading module is used for placing a reaction cup, when an instrument runs, the reaction cup loading module automatically heats and preserves heat, so that a sample in the reaction cup reacts with a reagent at the constant temperature of 37 ℃, and the reaction cup can directly carry out optical measurement.
The mixing and stirring module applies reverse air pressure to the opening end of the reaction cup to enable the reaction solution in the reaction cup to be stirred up and down, so that the sample and the reagent in the reaction solution are uniformly mixed. The mixing and stirring module does not need to use a traditional stirring rod directly contacting with the reaction solution, so that the cross contamination caused by incomplete cleaning of the stirring rod is avoided.
The reaction cup cleaning module is used for cleaning the reaction cup by using the preheated cleaning solution before the measurement is started, sucking out the reaction solution after the measurement is finished, and then washing the reaction cup by using the cleaning solution.
The optical module measures the intensity value of the monochromatic light with specific wavelength absorbed by the reaction solution when the reaction cup loading module automatically rotates.
As an optional scheme, the full-automatic quantitative urine detector further comprises a power module.
As an optional scheme, the fully-automatic quantitative urine detector further comprises an accessory device module.
As an optional scheme, the accessory device module comprises a cooling fan, a purification water barrel, a waste liquid collecting barrel and a cleaning liquid barrel.
As an optional scheme, the sample loading module, the sample dispensing module, the reagent loading module, the reagent dispensing module, the reaction cup loading module, the mixing and stirring module, the reaction cup cleaning module and the optical module operate according to a preset program.
As an optional solution, the preset program is: the method comprises the steps of loading a sample in a sample loading module, loading a reagent in a reagent loading module, automatically injecting the sample into a reaction cup by the sample injection module, automatically injecting the reagent into the reaction cup by the reagent injection module, automatically stirring the sample and the reagent in the reaction cup uniformly by a mixing and stirring module, starting to rotate the reaction cup loading module, automatically measuring a light absorption value when the reaction cup passes through a measuring position of an optical module, measuring the light absorption value once after the reaction cup loading module rotates for a circle, and cleaning the reaction cup by a cleaning solution before the measurement is started and after the measurement is finished by a reaction cup cleaning module.
As an alternative, the computer module includes a computer hardware unit and a computer software unit.
The computer hardware unit can be a server, a desktop computer, a mobile computer or a smart phone; the computer software unit can be a special computer software program or a special mobile phone APP of the full-automatic quantitative urine detector.
The full-automatic quantitative urine detector and the data analysis device can be communicated with each other in a wired connection mode or a wireless connection mode, the computer module and the printer module can be communicated with each other in a wired connection mode or a wireless connection mode, and the wireless connection mode can be WIFI, GPRS or Bluetooth.
As an optional scheme, the urine fully quantitative clinical detection reagent used in cooperation comprises urine hormone series fully quantitative detection reagents, urine special protein series fully quantitative detection reagents, urine biochemical series fully quantitative detection reagents, urine therapeutic drug monitoring series fully quantitative detection reagents, urine drug series fully quantitative detection reagents and urine conventional project series fully quantitative detection reagents.
The target substances detected by the urine hormone series full-quantitative detection reagent comprise: estrogens and metabolites thereof, androgens and metabolites thereof, adrenocortical hormones and metabolites thereof, adrenomedullary hormones and metabolites thereof, thyroid hormones; the target substance detected by the urine special protein series full-quantitative detection reagent comprises: urinary microalbumin, α 1 microglobulin, β 2 microglobulin, k/λ light chain, urinary transferrin, retinol binding protein, immunoglobulin, neutrophil gelatinase-associated lipocalin; the target substance detected by the urine biochemical series full-quantitative detection reagent comprises: creatinine, N-acetyl- β -D-glucosaminidase, amylase, leukocyte esterase; the target substance detected by the urine treatment drug monitoring series full-quantitative detection reagent comprises: antibiotic drugs, antineoplastic drugs, immunosuppressant drugs, cardiovascular and cerebrovascular drugs, antiepileptic/psychiatric drugs, anti-asthma drugs; the target substance detected by the urine drug series full-quantitative detection reagent comprises: 6-acetylmorphine, amphetamine, barbiturates, benzodiazepines, buprenorphine, ***e, ecstasy, hydrocodone, methadone, methamphetamine, opium, cannabis; the target substance detected by the urine routine item series full-quantitative detection reagent comprises: total urine protein, uric acid, urea nitrogen, uric acid alkalinity, urobilinogen, urohemoglobin, urine sugar, urine bilirubin, urine ketone bodies, urine nitrite, urine specific gravity and urine vitamin.
The invention provides a fully quantitative detection and analysis method for urine based on the fully quantitative detection and analysis system for urine, which comprises the following steps:
the monochromatic light with specific wavelength emitted by the optical module passes through a reaction cup filled with reaction solution, and the intensity value of the monochromatic light with specific wavelength absorbed by the reaction solution is in direct proportion to the concentration of the sample solution and the distance of the monochromatic light with specific wavelength passing through the reaction solution; the reaction solution is a mixed solution of a sample solution and a reagent; the calculation formula of the intensity value of the monochromatic light with the specific wavelength absorbed by the reaction solution is as follows:
wherein:
a is the intensity value absorbed by the reaction solution when monochromatic light with specific wavelength passes through the reaction solution;
t is the ratio of the intensity of transmitted light to the intensity of incident light, i.e. the light transmittance I t /I 0 ；
I 0 Is the intensity of the incident light;
I t is the transmitted light intensity;
epsilon is the molar absorption coefficient of the sample solution;
c is the molar concentration of the sample solution;
b is the thickness of the reaction solution layer;
the thickness b of the reaction solution layer is fixed and known by a full-automatic quantitative urine detector, the molar absorptivity epsilon of the sample solution is a coefficient related to the wavelength of the monochromatic light with a specific wavelength, the type of the sample solution and the temperature of the reaction solution, and when the temperature of the reaction solution is ensured to be constant, the molar concentration c of the sample solution and the intensity value of the monochromatic light with the specific wavelength absorbed by the reaction solution are in a linear relation.
Drawings
The contents of the drawings and the references therein are briefly described as follows:
FIG. 1 is a schematic view of the present invention.
In the figure: 1 is a full-automatic quantitative detector for urine, 11 is a sample loading module, 12 is a sample dispensing module, 13 is a reagent loading module, 14 is a reagent dispensing module, 15 is a reaction cup loading module, 16 is a mixing and stirring module, 17 is a reaction cup cleaning module, 18 is an optical module, 110 is a power supply module, 120 is an accessory device module, 121 is a cooling fan, 122 is a purification bucket, 123 is a waste liquid collecting bucket, and 124 is a cleaning liquid bucket; 2 is a data analysis device, 21 is a computer module, 22 is a printer module, 211 is a computer hardware unit, and 212 is a computer software unit; 3 is a complete quantitative clinical detection reagent for urine, 31 is a complete quantitative detection reagent for urine hormone series, 32 is a complete quantitative detection reagent for urine special protein series, 33 is a complete quantitative detection reagent for urine biochemical series, 34 is a complete quantitative detection reagent for urine therapeutic drug monitoring series, 35 is a complete quantitative detection reagent for urine drug series, and 36 is a complete quantitative detection reagent for urine conventional project series.
Detailed Description
The present invention is further described with reference to the drawings and examples below to assist those skilled in the art to further understand the technical solutions of the present invention. It should be noted that the contents of the examples are only examples for clearly illustrating the present invention, and should not be construed as limiting the scope of the present invention. Any combination of the embodiments or technical features described below can be used to form new embodiments without conflict.
Example 1:
as shown in the attached drawings of the specification, the full-quantitative urine detection and analysis system comprises a full-automatic quantitative urine detector 1, a data analysis device 2 and a full-quantitative urine clinical detection reagent 3 which is matched with the full-automatic quantitative urine detector.
In this embodiment, the fully automatic quantitative urine detector 1 includes a sample loading module 11, a sample dispensing module 12, a reagent loading module 13, a reagent dispensing module 14, a cuvette loading module 15, a mixing and stirring module 16, a cuvette cleaning module 17, and an optical module 18; the data analysis device 2 includes a computer module 21 and a printer module 22.
The sample loading module 11 is used for placing a sample cup or a test tube, and the sample cup or the test tube is transferred to a position where a sample is sucked by a sample needle through automatic rotation.
The sample dispensing module 12 is provided with a sample needle, the sample needle is provided with a liquid level sensor, a sample with a designated volume is sucked from a sample cup or a test tube, the sample dispensing module 12 moves the sample needle to the position of a reaction cup and fills the sample into the reaction cup, and after the sample is filled, the sample needle automatically returns to a cleaning tank to clean the inner wall and the outer wall of the cleaning tank.
The reagent loading module 13 is used for placing a reagent, a diluent and a cleaning solution, and conveying the reagent, the diluent and the cleaning solution to a reagent needle sample sucking position through automatic rotation; the reagent loading module 13 may also be used to store reagents cryogenically, with the reagents in the reagent loading module 13 being in a refrigerated state when power is turned on.
The reagent dispensing module 14 is provided with two reagent needles, the reagent needles are provided with liquid level sensors and absorb reagents with specified volumes from reagent bottles, the reagent dispensing module 14 moves the reagent needles to the positions of reaction cups and fills the reagents into the reaction cups, and after the reagent filling is finished, the reagent needles automatically return to a cleaning tank to clean the inner and outer walls of the cleaning tank; when the instrument is in operation, the reagent needle for the first reagent fill is on the opposite side when the reagent needle for the second reagent fill is in the wash position.
The reaction cup loading module 15 is used for placing a reaction cup, when an instrument runs, the reaction cup loading module 15 automatically heats and preserves heat, so that a sample in the reaction cup and a reagent react at the constant temperature of 37 ℃, and the reaction cup can directly carry out optical measurement.
The mixing and stirring module 16 applies reverse air pressure to the open end of the reaction cup to stir the reaction solution in the reaction cup up and down, so as to mix the sample in the reaction solution with the reagent. The mixing module 16 does not require a conventional stir bar that directly contacts the reaction solution, thereby avoiding cross-contamination caused by incomplete cleaning of the stir bar.
The reaction cup cleaning module 17 cleans the reaction cup with the preheated cleaning solution before starting the measurement, sucks out the reaction solution after the measurement is finished, and then washes the reaction cup with the cleaning solution.
The optical module 18 measures an intensity value of the monochromatic light of a specific wavelength absorbed by the reaction solution when the reaction cup loading module 15 is automatically rotated.
In this embodiment, the fully automatic quantitative urine detector 1 further includes a power module 110.
In this embodiment, the fully automatic quantitative urine detector 1 further includes an attachment module 120.
In this embodiment, the accessory module 120 includes a heat dissipation fan 121, a purification water tank 122, a waste liquid collection tank 123, and a cleaning liquid tank 124.
In the present embodiment, the sample loading module 11, the sample dispensing module 12, the reagent loading module 13, the reagent dispensing module 14, the cuvette loading module 15, the mixing module 16, the cuvette washing module 17, and the optical module 18 are operated according to a preset program.
In this embodiment, the preset program is: the method comprises the steps of loading a sample in a sample loading module 11, loading a reagent in a reagent loading module 13, automatically injecting the sample in a reaction cup by a sample injection module 12, automatically injecting the reagent in the reaction cup by a reagent injection module 14, automatically and uniformly stirring the sample and the reagent in the reaction cup by a mixing and stirring module 16, starting to rotate a reaction cup loading module 15, automatically measuring a light absorption value when the reaction cup passes through an optical module 18 to measure the position, measuring the light absorption value once after the reaction cup loading module 15 rotates for one circle, and cleaning the reaction cup by a cleaning solution before the measurement is started and after the measurement is finished by a reaction cup cleaning module 17.
In this embodiment, the computer module 21 includes a computer hardware unit 211 and a computer software unit 212.
The computer hardware unit 211 may be a server, a desktop computer, a mobile computer, or a smart phone; the computer software unit 212 may be a dedicated computer software program or a dedicated mobile phone APP for the full-automatic quantitative urine detector.
The full-automatic quantitative urine detector 1 can communicate with the data analysis device 2 in a wired or wireless connection mode, the computer module 21 and the printer module 22 can communicate with each other in a wired or wireless connection mode, and the wireless connection mode can be WIFI, GPRS or Bluetooth.
In this embodiment, the urine fully quantitative clinical detection reagent 3 used in cooperation includes a urine hormone series fully quantitative detection reagent 31, a urine specific protein series fully quantitative detection reagent 32, a urine biochemical series fully quantitative detection reagent 33, a urine therapeutic drug monitoring series fully quantitative detection reagent 34, a urine drug series fully quantitative detection reagent 35, and a urine routine project series fully quantitative detection reagent 36.
The target substances detected by the urine hormone series full-quantitative detection reagent 31 comprise: estrogen and its metabolites, androgen and its metabolites, adrenocortical hormone and its metabolites, adrenomedullary hormone and its metabolites, thyroid hormone; the target substances detected by the urine special protein series full-quantitative detection reagent 32 comprise: urinary microalbumin, α 1 microglobulin, β 2 microglobulin, k/λ light chain, urinary transferrin, retinol binding protein, immunoglobulin, neutrophil gelatinase-associated lipocalin; the target substances detected by the urine biochemical series full-quantitative detection reagent 33 comprise: creatinine, N-acetyl- β -D-glucosaminidase, amylase, leukocyte esterase; the target substances detected by the urine therapeutic drug monitoring series full-quantitative detection reagent 34 comprise: antibiotic drugs, antineoplastic drugs, immunosuppressant drugs, cardiovascular and cerebrovascular drugs, antiepileptic/psychiatric drugs, anti-asthma drugs; the target substances detected by the urine drug series full-quantitative detection reagent 35 comprise: 6-acetylmorphine, amphetamine, barbiturates, benzodiazepines, buprenorphine, ***e, ecstasy, hydrocodone, methadone, methamphetamine, opium, cannabis; the target substances detected by the urine routine item series full-quantitative detection reagent 36 comprise: total urine protein, uric acid, urea nitrogen, uric acid alkalinity, urobilinogen, urohemoglobin, urine sugar, urine bilirubin, urine ketone bodies, urine nitrite, urine specific gravity and urine vitamin.
Example 2:
a urine fully quantitative detection analysis method based on the urine fully quantitative detection analysis system, the analysis method comprising:
the monochromatic light with specific wavelength emitted by the optical module passes through a reaction cup filled with reaction solution, and the intensity value of the monochromatic light with specific wavelength absorbed by the reaction solution is in direct proportion to the concentration of the sample solution and the distance of the monochromatic light with specific wavelength passing through the reaction solution; the reaction solution is a mixed solution of a sample solution and a reagent; the calculation formula of the intensity value of the monochromatic light with the specific wavelength absorbed by the reaction solution is as follows:
wherein:
a is the intensity value absorbed by the reaction solution when monochromatic light with specific wavelength passes through the reaction solution;
t is the ratio of the intensity of transmitted light to the intensity of incident light, i.e. the transmittance I t /I 0 ；
I 0 Is the intensity of the incident light;
I t is the transmitted light intensity;
epsilon is the molar absorption coefficient of the sample solution;
c is the molar concentration of the sample solution;
b is the thickness of the reaction solution layer;
the thickness b of the reaction solution layer is fixed and known by a full-automatic quantitative urine detector, the molar absorptivity epsilon of the sample solution is a coefficient related to the wavelength of the monochromatic light with a specific wavelength, the type of the sample solution and the temperature of the reaction solution, and when the temperature of the reaction solution is ensured to be constant, the molar concentration c of the sample solution and the intensity value of the monochromatic light with the specific wavelength absorbed by the reaction solution are in a linear relation.
The technical characteristics form a preferred embodiment of the invention, which has stronger adaptability and better implementation effect, and the invention can change or increase or decrease unnecessary technical characteristics according to actual conditions to meet the requirements of different conditions.
It will be apparent to those skilled in the art that other variations and modifications may be made on the above-described embodiments. All insubstantial modifications, or direct application of the inventive concepts and solutions to other applications without modification, which fall within the scope of the present invention.
Claims (10)
1. The full-quantitative urine detection and analysis system is characterized by comprising a full-automatic urine quantitative detector, a data analysis device and a full-quantitative urine clinical detection reagent matched with the full-automatic urine quantitative detector and the data analysis device.
2. The full quantitative detection and analysis system for urine according to claim 1, wherein the full automatic quantitative detection instrument for urine comprises a sample loading module, a sample dispensing module, a reagent loading module, a reagent dispensing module, a reaction cup loading module, a mixing and stirring module, a reaction cup cleaning module and an optical module; the data analysis device comprises a computer module and a printer module.
3. The full quantitative detection and analysis system for urine according to claim 2, wherein the full automatic quantitative detection instrument for urine further comprises a power module.
4. The full-quantitative urine detection and analysis system according to claim 2, wherein the full-automatic quantitative urine detector further comprises an attachment module.
5. The urine full-quantitative detection and analysis system according to claim 4, wherein the accessory module comprises a cooling fan, a purification water barrel, a waste liquid collecting barrel and a cleaning liquid barrel.
6. The full quantitative detection and analysis system for urine according to claim 2, wherein the sample loading module, the sample dispensing module, the reagent loading module, the reagent dispensing module, the reaction cup loading module, the mixing and stirring module, the reaction cup cleaning module and the optical module operate according to a preset program.
7. The urine fully quantitative detection and analysis system according to claim 6, wherein the preset program is: the method comprises the steps of loading a sample in a sample loading module, loading a reagent in a reagent loading module, automatically injecting the sample into a reaction cup by the sample injection module, automatically injecting the reagent into the reaction cup by the reagent injection module, automatically stirring the sample and the reagent in the reaction cup uniformly by a mixing and stirring module, starting to rotate the reaction cup loading module, automatically measuring a light absorption value when the reaction cup passes through a measuring position of an optical module, measuring the light absorption value once after the reaction cup loading module rotates for a circle, and cleaning the reaction cup by a cleaning solution before the measurement is started and after the measurement is finished by a reaction cup cleaning module.
8. The full quantitative detection and analysis system for urine according to claim 2, wherein the computer module comprises a computer hardware unit and a computer software unit.
9. The urine fully quantitative detection and analysis system according to claim 1, wherein the urine fully quantitative clinical detection reagent used in cooperation comprises urine hormone series fully quantitative detection reagent, urine special protein series fully quantitative detection reagent, urine biochemical series fully quantitative detection reagent, urine therapeutic drug monitoring series fully quantitative detection reagent, urine drug series fully quantitative detection reagent, urine routine project series fully quantitative detection reagent.
10. A urine fully quantitative detection analysis method based on the urine fully quantitative detection analysis system according to any one of claims 1 to 9, wherein the analysis method comprises:
the monochromatic light with specific wavelength emitted by the optical module passes through a reaction cup filled with reaction solution, and the intensity value of the monochromatic light with specific wavelength absorbed by the reaction solution is in direct proportion to the concentration of the sample solution and the distance of the monochromatic light with specific wavelength passing through the reaction solution; the reaction solution is a mixed solution of a sample solution and a reagent; the calculation formula of the intensity value of the monochromatic light with the specific wavelength absorbed by the reaction solution is as follows:
wherein:
a is the intensity value absorbed by the reaction solution when monochromatic light with specific wavelength passes through the reaction solution;
t is the ratio of the intensity of transmitted light to the intensity of incident light, i.e. the light transmittance I t /I 0 ；
I 0 Is the incident light intensity;
I t is the transmitted light intensity;
epsilon is the molar absorption coefficient of the sample solution;
c is the molar concentration of the sample solution;
b is the thickness of the reaction solution layer;
the thickness b of the reaction solution layer is fixed and known by a full-automatic quantitative urine detector, the molar absorption coefficient epsilon of the sample solution is a coefficient related to the wavelength of the monochromatic light with the specific wavelength, the type of the sample solution and the temperature of the reaction solution, and when the temperature of the reaction solution is ensured to be constant, the molar concentration c of the sample solution and the intensity value of the monochromatic light with the specific wavelength absorbed by the reaction solution are in a linear relation.
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