CN115193728A - Microparticle sorting device - Google Patents

Microparticle sorting device Download PDF

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Publication number
CN115193728A
CN115193728A CN202210859824.9A CN202210859824A CN115193728A CN 115193728 A CN115193728 A CN 115193728A CN 202210859824 A CN202210859824 A CN 202210859824A CN 115193728 A CN115193728 A CN 115193728A
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China
Prior art keywords
particle
particle sample
sample
section
branch
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CN202210859824.9A
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Chinese (zh)
Inventor
冯世杰
焦少灼
李宗文
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Beijing Xunyin Biological Technology Co ltd
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Beijing Xunyin Biological Technology Co ltd
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Priority to CN202210859824.9A priority Critical patent/CN115193728A/en
Publication of CN115193728A publication Critical patent/CN115193728A/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
    • B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
    • B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
    • B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
    • B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
    • B07C5/02—Measures preceding sorting, e.g. arranging articles in a stream orientating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
    • B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
    • B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
    • B07C5/04—Sorting according to size
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
    • B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
    • B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
    • B07C5/34—Sorting according to other particular properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
    • B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
    • B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
    • B07C5/36—Sorting apparatus characterised by the means used for distribution
    • B07C5/363—Sorting apparatus characterised by the means used for distribution by means of air
    • B07C5/367—Sorting apparatus characterised by the means used for distribution by means of air using a plurality of separation means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
    • B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
    • B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
    • B07C5/36—Sorting apparatus characterised by the means used for distribution
    • B07C5/38—Collecting or arranging articles in groups

Abstract

The invention discloses a microparticle sorting device, which comprises a seat body, a driving device, a detection device, a sorting device and a control device, wherein the seat body is provided with a seat body; the base body is provided with a sample flow channel for the particle sample to flow, the sample flow channel comprises an inlet section and a plurality of outlet sections, and the outlet sections are arranged in different directions; the driving device is used for driving the particle sample to flow along the sample flow channel; the detection device is arranged in the midstream of the inlet section and is used for detecting the particle sample flowing through so as to identify the parameter information of the particle sample, wherein the parameter information comprises the size of the particle sample and the content of a target substance in the particle sample; the sorting device is arranged at the downstream of the inlet section and used for driving the particle sample to move along the radial direction of the inlet section so as to adjust the flow direction of the particle sample, so that the particle sample can flow to the corresponding outlet section; the control device comprises a controller which is electrically connected with the detection device and the sorting device; by such an arrangement, sorting of target particles can be achieved.

Description

Microparticle sorting device
Technical Field
The invention relates to the technical field of microparticle sorting, in particular to a microparticle sorting device.
Background
Flow cytometers are commonly used to identify various types of cells and biological fluids. Existing flow cytometers are typically made of quartz, with optically transparent flow chambers formed as flow channels through which an identified cell stream should flow. The cell flow flowing in the flow channel is collected by a sheath fluid concentrically surrounding the cell flow and flows into the center of the flow channel. The center of the flow channel is irradiated with a laser beam, and when a cell passes through the irradiated region, light is scattered depending on the size, shape, and refractive index of the cell. The laser wavelength is determined in coordination with the type of fluorochrome to detect cells specifically stained with the fluorochrome using fluorescence. In this way, cells can be analyzed in many ways by detecting fluorescence from different wavelengths with a plurality of photodetectors in addition to scattered light for each cell.
The existing micro-particle separation device partially separates by a mode of discharging liquid drops through a nozzle, and when a sample is a cell polluted by pathogenic germs or bacteria, the mode has the risk of spreading dangerous substances into the atmosphere as aerosol, so that the problem of biohazard exists; meanwhile, since the density of non-target cells is very high compared with that of target cells, the cell population contains more non-target cells than target cells after separation, and therefore, part of the micro-particle separation device can perform sorting processing on the recovered sample again; repeated sorting increases the probability of target cell loss due to the small absolute number of target cells.
Disclosure of Invention
The invention mainly aims to provide a micro-particle sorting device, aiming at solving the problems that the existing micro-particle separating device has risks of biohazard and has higher target cell loss probability.
To achieve the above object, the present invention provides a microparticle sorting device, including:
the base body is provided with a sample flow channel for flowing a particle sample, the sample flow channel is arranged in a sealing manner, the sample flow channel comprises an inlet section and a plurality of outlet sections, and the outlet sections are arranged in different directions;
a drive means for driving the particle sample to flow along the sample flow path;
the detection device is arranged in the midstream of the inlet section and is used for detecting the particle sample flowing through so as to identify the parameter information of the particle sample, wherein the parameter information comprises the size of the particle sample and the content of the target substance in the particle sample;
the sorting device is arranged at the downstream of the inlet section and is used for driving the particle sample to move along the radial direction of the inlet section so as to adjust the flow direction of the particle sample, so that the particle sample can flow to the corresponding outlet section; and the number of the first and second groups,
and the control device comprises a controller, and the controller is electrically connected with the detection device and the sorting device.
Optionally, the inducer includes:
a particle segment for flow of the particle sample;
the two sheath liquid sections are used for flowing sheath liquid, the two sheath liquid sections and the particle section are arranged side by side and are respectively arranged at two sides of the particle section, and the part of each sheath liquid section close to the outlet of the sheath liquid section and the particle section form an included angle; and (c) a second step of,
a confluence section, the inlets of which are respectively connected with the particle sections and the outlets of the two sheath liquid sections, the outlets of which are respectively connected with the outlet sections, wherein the confluence section is used for flowing the particle sample coated with the sheath liquid;
the detection device is arranged in the midstream of the confluence section;
the sorting device is arranged at the downstream of the confluence section.
Optionally, the driving device comprises a gas supply device, the gas supply device comprising:
driving an air source;
a first gas channel, an inlet of the first gas channel is connected with the driving gas source, and an outlet of the first gas channel is communicated with the upstream of the particle section, so that when the driving gas source is opened, the particle sample can be driven to flow along the particle section; and (c) a second step of,
and the inlet of the second air passage is connected with the driving air source, and the outlet of the second air passage is respectively communicated with the two sheath liquid sections, so that when the driving air source is opened, the sheath liquid can be driven to flow.
Optionally, a plurality of export sections are including the three branch road that sets up side by side, adjacent two be the contained angle setting between the branch road, three branch road includes first branch road, second branch road and third branch road, first branch road with the third branch road is respectively certainly the relative both sides orientation of induction zone extends towards the direction that deviates from each other.
Optionally, the diameter of the inlet section is D and the diameter of the first leg is D 1 And satisfies the following relationship: d 1 ≦D≦2D 1 (ii) a And/or the presence of a gas in the gas,
the diameter of the inlet section is D, and the diameter of the second branch is D 2 And satisfies the following relationship: d 2 ≦D≦2D 2 (ii) a And/or the presence of a gas in the gas,
the diameter of the inlet section is D, and the diameter of the third branch is D 3 And satisfies the following relationship: d 3 ≦D≦2D 3 。
Optionally, a first diversion trench is formed in the inner wall, close to the first branch, of the inlet section, and an arc-shaped diversion surface is formed on the bottom wall of the first diversion trench; and/or the presence of a gas in the atmosphere,
the inlet section is provided with a second diversion trench close to the inner wall of the third branch, and the bottom wall of the second diversion trench is provided with an arc diversion surface.
Optionally, the inlet section is provided with a first air inlet at a side close to the first branch, and a second air inlet at a side close to the third branch;
the sorting device comprises a sorting air source, a first valve and a second valve, wherein the first valve and the second valve are respectively and correspondingly arranged on the first air inlet and the second air inlet;
the controller is electrically connected with the first valve, the second valve and the detection device, so as to control the first valve and the second valve to be selectively opened after the detection device identifies the parameter information of the particle sample, so that the first air inlet and the second air inlet are correspondingly communicated with the air source.
Optionally, a magnetizing flow channel is further formed on the base body, the magnetizing flow channel is used for flowing a magnetic reagent, and the magnetizing flow channel is located at the upstream of the inlet section and is communicated with the inlet section so as to coat the particle sample with the magnetic reagent;
the sorting device comprises a magnetic suction assembly, and the magnetic suction assembly comprises a first electromagnet and a second electromagnet which are respectively arranged on two sides of the inlet section;
the controller is electrically connected with the first electromagnet, the second electromagnet and the detection device, and is used for controlling the coil of the first electromagnet and the coil of the second electromagnet to be selectively electrified after the detection device identifies the parameter information of the particle sample, so that magnetic fields are respectively and correspondingly and selectively generated on two sides of the inlet section.
Optionally, the detection device comprises an optical detection mechanism, the optical detection mechanism comprising:
the optical detector is arranged on the base and used for detecting the size of the particle sample and the content of the target substance in the particle sample; and the number of the first and second groups,
an analyzer in electrical communication with the optical detector to identify a type of the particle sample;
wherein the controller is electrically connected to the analyzer and the sorting device.
Optionally, the optical detector comprises:
the light source device is arranged above the seat body, and the light emitting end of the light source device faces the inlet section and is used for irradiating the particle sample flowing through so as to enable the particle sample to generate scattered light and fluorescence;
a first detector for receiving the scattered light to detect a size of the particle sample;
a second detector for receiving the fluorescence to detect the content of the target substance in the particle sample;
wherein the analyzer is electrically connected to the first detector and the second detector.
According to the technical scheme, the sample flow channel is formed in the base body in a sealing mode and is used for the particle sample to flow, so that when the particle sample is a cell polluted by pathogenic germs or bacteria, dangerous substances can be effectively prevented from being spread into the atmosphere in an aerosol mode, the problem of biohazard can be solved, and the environmental protection performance of the microparticle sorting device is improved; meanwhile, a driving device is arranged to drive the particle sample to flow along the sample flow channel, the sample flow channel comprises an inlet section and a plurality of outlet sections, the detecting device is arranged at the midstream of the inlet section to detect the particle sample flowing through, and the sorting device is arranged at the downstream of the inlet section to drive the particle sample to move along the radial direction of the inlet section so as to adjust the flow direction of the particle sample, so that the particle sample can flow to the corresponding outlet sections; by such arrangement, the target particle sample is sorted; that is, during the sorting process, when the particle sample flows through the inlet section, the detection device detects the particle sample and identifies the parameter information of the particle sample (i.e. the size of the particle sample and the content of the target substance in the particle sample), and after the controller obtains the parameter information of the particle sample, the controller controls the sorting device to drive the particle sample to move so as to flow to the corresponding outlet section; according to the arrangement, the microparticle sorting device is simple in structure and concise in sorting steps, and through the combined action of the detection device and the sorting device, the particle sample sorting effect is good, repeated sorting is not needed, and the target cell loss probability is reduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
Fig. 1 is a schematic structural diagram of an embodiment of a microparticle sorting device according to the present invention;
FIG. 2 is a schematic view of the sorting apparatus of FIG. 1 (in an open state);
FIG. 3 is an enlarged schematic view of detail A of FIG. 2;
FIG. 4 is a schematic view of the sorting apparatus of FIG. 1 (closed state);
FIG. 5 is an enlarged schematic view of detail B of FIG. 4;
fig. 6 is a sorting flow chart of the microparticle sorting device (first embodiment) in fig. 1;
fig. 7 is a sorting flow chart of the microparticle sorting device (second embodiment) in fig. 1.
The embodiment of the invention is illustrated by reference numerals:
Figure BDA0003757357790000051
Figure BDA0003757357790000061
the implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that, if directional indications (such as upper, lower, left, right, front, rear, 8230; \8230;) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative positional relationship between the components in a specific posture (as shown in the figure), the motion situation, etc., and if the specific posture is changed, the directional indications are correspondingly changed.
In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the meaning of "and/or" appearing throughout includes three juxtapositions, exemplified by "A and/or B" including either A or B or both A and B. In addition, technical solutions between the embodiments may be combined with each other, but must be based on the realization of the technical solutions by a person skilled in the art, and when the technical solutions are contradictory to each other or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
Flow cytometers are commonly used to identify various types of cells and biological fluids. Existing flow cytometers are typically made of quartz, with optically transparent flow chambers formed as flow channels through which an identified cell stream should flow. The cell flow flowing in the flow channel is collected by a sheath fluid concentrically surrounding the cell flow to flow in the center portion of the flow channel. The center of the flow channel is irradiated with a laser beam, and when a cell passes through the irradiated region, light is scattered depending on the size, shape, and refractive index of the cell. The laser wavelength is determined in coordination with the type of fluorochrome to detect cells specifically stained with the fluorochrome using fluorescence. In this way, by detecting fluorescence from different wavelengths using a plurality of photodetectors in addition to scattered light for each cell, the cells can be analyzed in a variety of ways.
The existing micro-particle separation device partially separates by a mode of discharging liquid drops through a nozzle, and when a sample is a cell polluted by pathogenic germs or bacteria, the mode has the risk of spreading dangerous substances into the atmosphere as aerosol, so that the problem of biohazard exists; meanwhile, since the density of non-target cells is very high compared with that of target cells, the cell population contains more non-target cells than target cells after separation, and therefore, part of the micro-particle separation device can perform sorting processing on the recovered sample again; repeated sorting increases the probability of target cell loss due to the small absolute number of target cells.
In view of the above, the present invention provides a micro-particle sorting apparatus. Fig. 1 to 7 show an embodiment of a microparticle sorting device according to the present invention.
Referring to fig. 1 to 5, the microparticle sorting device 100 includes a base 1, a driving device 2, a detecting device 3, a sorting device 4, and a control device (not shown in the drawings); the holder body 1 is formed with a sample flow channel 11 for flowing the particle sample, the sample flow channel 11 is hermetically arranged, the sample flow channel 11 includes an inlet section 111 and a plurality of outlet sections 112, and the plurality of outlet sections 112 are arranged in different directions; the driving device 2 is used for driving the particle sample to flow along the sample flow channel 11; the detection device 3 is arranged in the middle of the inlet section 111 and is used for detecting the particle sample flowing through to identify the parameter information of the particle sample, wherein the parameter information comprises the size of the particle sample and the content of the target substance in the particle sample; the sorting device 4 is disposed downstream of the inlet section 111, and is configured to drive the particle sample to move along the radial direction of the inlet section 111, so as to adjust the flow direction of the particle sample, so that the particle sample can flow to the corresponding outlet section 112; the control means comprise a controller (not shown in the figures) which is electrically connected to the detection means 3 and to the sorting means 4.
In the technical scheme of the invention, the sample flow channel 11 is formed on the base body 1 in a sealing manner, and the sample flow channel 11 is used for the particle sample to flow, so that when the particle sample is a cell polluted by pathogenic germs or bacteria, dangerous substances can be effectively prevented from being spread and scattered into the atmosphere in the form of aerosol, and therefore, the problem of biohazard can be caused, and the environmental protection performance of the microparticle sorting device 100 is improved; meanwhile, a driving device 2 is arranged to drive the particle sample to flow along the sample flow channel 11, the sample flow channel 11 comprises an inlet section 111 and a plurality of outlet sections 112, the detecting device 3 is arranged at the midstream of the inlet section 111 to detect the particle sample flowing through, the sorting device 4 is arranged at the downstream of the inlet section 111 to drive the particle sample to move along the radial direction of the inlet section 111 to adjust the flow direction of the particle sample, so that the particle sample can flow to the corresponding outlet section 112; by the arrangement, the target particle sample is sorted; that is, during the sorting process, when the particle sample flows through the inlet section 111, the detection device 3 detects the particle sample and identifies the parameter information of the particle sample (i.e. the size of the particle sample and the content of the target substance in the particle sample), and after the controller obtains the parameter information of the particle sample, the controller controls the sorting device 4 to drive the particle sample to move so as to flow to the corresponding outlet section 112; so set up, microparticle sorting unit 100 simple structure, it is succinct to select separately the step, through detection device 3 with sorting unit 4's combined action for particle sample selects separately effectually, need not to carry out repeated sorting, thereby reduces the target cell and loses the probability.
With further reference to fig. 2, the inlet section 111 comprises a particle section 1111, two sheath fluid sections 1112, and a confluence section 1113; the particle segment 1111 is used for the particle sample to flow; the two sheath fluid segments 1112 are used for flowing sheath fluid, the two sheath fluid segments 1112 and the particle segment 1111 are arranged side by side and are respectively arranged at two sides of the particle segment 1111, and the part of each sheath fluid segment 1112 close to the outlet thereof forms an included angle with the particle segment 1111; the confluence segment 1113 has an inlet connected to the particle segment 1111 and the outlets of the two sheath fluid segments 1112, respectively, and has an outlet connected to the plurality of outlet segments 112, respectively, wherein the confluence segment 1113 is used for flowing the particle sample coated with the sheath fluid; wherein the detection device 3 is arranged in the midstream of the confluence section 1113; the sorting device 4 is arranged at the downstream of the confluence segment 1113; the sheath solution is a balanced electrolyte solution without a fluorescence background, mainly contains sodium chloride, potassium chloride, disodium edetate and a bacteriostatic agent, and is used as the sheath solution when a Coulter flow cytometer is used for analyzing the physicochemical and biological characteristics of biological particles such as cells. Most high-end blood analyzers use the sheath flow principle to improve the detection accuracy, and the main reagent is sheath fluid. The sheath flow mode comprises single sheath flow and multi-sheath flow, and aims to enable cells to pass through the detection part in a straight line; that is, in the present embodiment, the inlet section 111 is divided into a particle section 1111 in which the particle sample in an initial state flows, two sheath liquid sections 1112 in which the sheath liquid flows, and a merging section 1113 in which the sheath liquid flows around the particle sample when the particle section 1111 and the two sheath liquid sections 1112 merge into the merging section 1113; meanwhile, since the two sheath fluid segments 1112 are arranged side by side with the particle segment 1111 and are separately arranged on both sides of the particle segment 1111, and the portion of each sheath fluid segment 1112 near the outlet thereof is arranged at an angle with the particle segment 1111, when the driving device 2 drives the particle sample and the sheath fluid, the sheath fluid can flow at high speed around the particle sample to form a circular stream, the particle sample is arranged in a single row under the sheath fluid and sequentially flows through the detection device 3, and the coaxial flow design makes the stream formed by the particle sample stream and the sheath fluid stream always maintain a layered sheath stream state, so that the microparticle sorting device 100 can not only detect the particle sample more accurately, but also make the particle sample stream and the sheath fluid stream more easily separate after sorting.
In the present embodiment, the driving device 2 includes a gas supply device 21, and the gas supply device 21 includes a driving gas source (not shown in the figure), a first gas passage 211, and a second gas passage 212; the inlet of the first gas channel 211 is connected to the driving gas source, and the outlet thereof is connected to the upstream of the particle segment 1111, so as to drive the particle sample to flow along the particle segment 1111 when the driving gas source is opened; the inlet of the second air channel 212 is connected to the driving air source, and the outlets thereof are respectively communicated with the two sheath liquid segments 1112, so as to drive the sheath liquid to flow when the driving air source is opened; the driving gas source is arranged to simultaneously drive the particle sample and the sheath fluid, so that the structure is simple and the cost is low; meanwhile, when positive pressure gas pressure is applied to the particle segment 1111 and the sheath fluid segment 1112 at the same time, the sheath fluid can form vortex at the confluence of the particle segment 1111 and the sheath fluid segment 1112, so that the particle sample can be wrapped and flowed at high speed.
In the present invention, the plurality of outlet sections 112 include three branches arranged side by side, an included angle is formed between two adjacent branches, the three branches include a first branch 1121, a second branch 1122, and a third branch 1123, and the first branch 1121 and the third branch 1123 extend from two opposite sides of the inlet section 111 toward directions away from each other, respectively; that is, the particle samples are classified into three categories according to the parameter information of the particle samples (i.e., the size of the particle samples and the content of the target substance in the particle samples), so as to respectively correspond to the first branch 1121, the second branch 1122 and the third branch 1123, thereby further improving the sorting effect of the particle samples.
Specifically, in the present invention, the diameter of the inlet section 111 is D, and the diameter of the first branch 1121 is D 1 And satisfies the following relationship: d 1 ≦D≦2D 1 (ii) a In this way, the first branch 1121 may allow the corresponding particle sample to pass through, so as to achieve sorting of the particle sample; at the same time, the inlet section 111 may allow one particle sample to pass through, but not two particle samples to pass through side by side along the radial direction, so that the flow of the particle samples is more regular, of the detection device 3The detection data is more accurate.
Specifically, in the present invention, the diameter of the inlet section 111 is D, and the diameter of the second branch 1122 is D 2 And satisfies the following relationship: d 2 ≦D≦2D 2 (ii) a In this way, the second branch 1122 can allow the corresponding particle sample to pass through, thereby achieving sorting of the particle sample; at the same time, the inlet section 111 may allow one particle sample to pass through, but may not allow two particle samples to pass through side by side along the radial direction, so that the flow of the particle samples is more regular, and the detection data of the detection device 3 is more accurate.
Specifically, in the present invention, the diameter of the inlet section 111 is D, and the diameter of the third branch 1123 is D 3 And satisfies the following relationship: d 3 ≦D≦2D 3 (ii) a In this way, the third branch 1123 can allow the corresponding particle sample to pass through, thereby realizing sorting of the particle sample; at the same time, the inlet section 111 may allow one particle sample to pass through, but may not allow two particle samples to pass through side by side along the radial direction, so that the flow of the particle samples is more regular, and the detection data of the detection device 3 is more accurate.
It should be noted that the above three technical features may be set in one, two or simultaneously; specifically, in the present embodiment, the above three technical features are simultaneously provided, that is, the diameter of the inlet section 111 is D, and the diameter of the first branch 1121 is D 1 The diameter of the second branch 1122 is D 2 The diameter of the third branch 1123 is D 3 And satisfies the following relationship: d 1 ≦D≦2D 1 、D 2 ≦D≦2D 2 、D 3 ≦D≦2D 3 (ii) a The arrangement is such that the first branch 1121, the second branch 1122, and the third branch 1123 can allow the corresponding particle sample to pass through, so as to realize sorting of the particle sample; at the same time, the inlet section 111 allows each of the particle samples to pass in sequence through the detection device 3 individually, thereby allowing the particles to pass throughThe flow of the subsamples is more regular and the detection data of the detection device 3 is more accurate.
Specifically, in the present invention, a first diversion trench is disposed on an inner wall of the inlet section 111 close to the first branch 1121, and an arc diversion surface is disposed on a bottom wall of the first diversion trench; guiding the particle sample flowing to the first branch 1121 correspondingly by arranging the first guiding gutter; simultaneously, the diapire of first guiding gutter sets up arc water conservancy diversion face for with the corresponding particle sample surface looks adaptation that makes, thereby make particle sample flow more smoothly, facilitate for sorting of particle sample.
Specifically, in the present invention, a second diversion trench is disposed on the inner wall of the inlet section 111 close to the third branch 1123, and an arc diversion surface is disposed on the bottom wall of the second diversion trench; guiding the particle sample flowing to the third branch 1123 correspondingly by arranging the second guide groove; simultaneously, the diapire of second guiding gutter sets up arc guiding surface for with the corresponding particle sample surface looks adaptation that makes corresponds, thereby make the particle sample flow that corresponds more smooth and easy, facilitate for sorting of particle sample.
It should be noted that the two technical features may be alternatively or simultaneously set, specifically, in this embodiment, the two technical features are simultaneously set, that is, a first diversion trench is formed in the inner wall of the inlet section 111 close to the first branch 1121, an arc diversion surface is formed on the bottom wall of the first diversion trench, a second diversion trench is formed in the inner wall of the inlet section 111 close to the third branch 1123, and an arc diversion surface is formed on the bottom wall of the second diversion trench; guiding the particle samples flowing to the first branch 1121 and the third branch 1123 correspondingly by arranging the first guide groove and the second guide groove; simultaneously, the diapire of first guiding gutter with the diapire of second guiding gutter all sets up arc water conservancy diversion face for with the corresponding particle sample surface looks adaptation that makes, thereby make the particle sample flow that corresponds more smooth and easy, facilitate for sorting of particle sample.
In this embodiment, with further reference to fig. 2 to 5, the inlet section 111 is provided with a first air inlet 1114 at a side close to the first branch 1121, and a second air inlet 1115 at a side close to the third branch 1123; the sorting device 4 comprises a sorting gas source (not shown), a first valve 41 and a second valve 42, wherein the first valve 41 and the second valve 42 are respectively arranged at the first gas inlet 1114 and the second gas inlet 1115; wherein the controller is electrically connected to the first valve 41, the second valve 42 and the detection device 3 to control the first valve 41 and the second valve 42 to selectively open after the detection device 3 identifies the parameter information of the particle sample, so that the first gas inlet 1114 and the second gas inlet 1115 are correspondingly communicated with the gas source; that is, the inlet section 111 is provided with the first inlet 1114 and the second inlet 1115 on two sides thereof, the first inlet 1114 and the second inlet 1115 correspond to the first branch 1121 and the third branch 1123, respectively, and the first inlet 1114 and the second inlet 1115 are provided with the first valve 41 and the second valve 42, respectively, after the particle sample flows through the detection apparatus 3, the detection apparatus 3 identifies parameter information of the particle sample, and the controller controls the first valve 41 and the second valve 42 to selectively open according to the parameter information of the particle sample, so that the gas source is selectively communicated with the inlet section 111 through the first inlet 1114 and the second inlet 1115, thereby enabling the particle sample to flow into the corresponding branch under the push of the gas flow, and further enabling the sorting of the particle sample.
In other embodiments of the present invention, a magnetizing flow channel is further formed on the base body 1, the magnetizing flow channel is used for flowing a magnetic reagent, and the magnetizing flow channel is located upstream of the entrance section 111 and is communicated with the entrance section 111 to coat the particle sample with the magnetic reagent; the sorting device 4 comprises a magnetic suction component, and the magnetic suction component comprises a first electromagnet and a second electromagnet which are respectively arranged on two sides of the inlet section 111; wherein the controller is electrically connected to the first electromagnet, the second electromagnet and the detection device 3, so as to control the coils of the first electromagnet and the second electromagnet to be selectively energized to respectively and selectively generate magnetic fields on two sides of the entrance section 111 after the detection device 3 identifies the parameter information of the particle sample; in this embodiment, the particle sample is coated with the magnetic reagent through the magnetic flow channel, the first electromagnet and the second electromagnet are respectively disposed on two sides of the inlet section 111, when the particle sample flows through the detection device 3, the detection device 3 identifies parameter information of the particle sample, and the controller controls the coil of the first electromagnet and the coil of the second electromagnet to be selectively energized according to the parameter information of the particle sample, so as to respectively and selectively generate magnetic fields on two sides of the inlet section 111, and thus the particle sample can be driven to move in the radial direction of the inlet section 111, so that the particle sample can flow into corresponding branches, and the particle sample can be sorted.
In the present invention, the detection device 3 comprises an optical detection mechanism 31, the optical detection mechanism 31 comprises an optical detector 311 and an analyzer (not shown in the figure); the optical detector 311 is disposed on the base 1 and configured to detect the size of the particle sample and the content of the target substance in the particle sample; the analyzer is in electrical communication with the optical detector 311 to identify the type of the particle sample; wherein the controller is electrically connected to the analyzer and the sorting device 4; that is, the size of the particle sample and the content of the target substance in the particle sample are detected by the optical detector 311, the analyzer identifies the type of the particle sample according to the size of the particle sample and the content of the target substance in the particle sample, and the controller controls the action of the sorting device 4 according to the type of the particle sample to realize the sorting of the particle sample.
Specifically, the optical detector 311 includes a light source device 3111, a first detector 3112, and a second detector 3113; the light source device 3111 is disposed above the seat body 1, and a light emitting end of the light source device is disposed toward the inlet section 111 for irradiating the particle sample flowing through, so that the particle sample generates scattered light and fluorescence; the first detector 3112 is configured to receive the scattered light to detect a size of the particle sample; the second detector 3113 is configured to receive the fluorescence to detect a content of a target substance in the particle sample; wherein the analyzer is electrically connected to the first detector 3112 and the second detector 3113; that is, after the particle sample is irradiated with the light emitted from the light source device 3111, the particle sample is excited to generate scattered light and fluorescence, the first detector 3112 detects the size of the particle sample according to the scattered light, and the second detector 3113 detects the content of the target substance in the particle sample according to the fluorescence.
The following description is given by way of specific examples: when the particle sample is a cell, the light source device 3111 is typically configured as a laser; the laser beam after focusing and shaping vertically irradiates on the particle sample flow, and the cell dyed by fluorescence generates scattered light and excited fluorescence under the irradiation of the laser beam. The two signals are simultaneously received by a photodiode which is arranged opposite to the laser in the vertical direction and a photomultiplier which is arranged at the same side of the photodiode and is vertical to the light propagation direction of the laser. Receiving scattered light signals by the photodiode, the scattered light signals reflecting the size of the cell volume; the receiving direction of the fluorescent signal is vertical to the light propagation direction of the laser, and the fluorescent signal is separated by a series of dichromatic reflectors and band-pass filters to form a plurality of fluorescent signals with different wavelengths, and the fluorescent signals are received by the photomultiplier tube, converted into electronic signals through integral amplification and inversion, input into an electronic information receiver and transmitted to a computer connected with the electronic information receiver by the electronic information receiver; the intensity of the fluorescence signal represents the content and the ploidy of the DNA in the tested cell nucleus, and the fluorescence signal is received by the photomultiplier tube and then converted into an electric signal, and then the electric signal is converted into a digital signal which can be identified by a computer through an analog/digital converter; thus, the detection of the particle sample can be realized.
In the present invention, the microparticle sorting apparatus 100 further includes a particle bin 5, a sheath fluid bin 6 and three collecting bins 7, which are disposed on the seat body 1, the particle bin 5 is used for storing a particle sample, the particle bin 5 corresponds to the particle segment 1111 and is disposed at the upstream of the particle segment 1111, and the particle bin 5 is communicated with the particle segment 1111; the sheath fluid chamber 6 is configured to store the sheath fluid, the sheath fluid chamber 6 corresponds to the two sheath fluid segments 1112 and is disposed upstream of the two sheath fluid segments 1112, and the sheath fluid chamber 6 is respectively communicated with the two sheath fluid segments 1112; the three collecting bins 7 are used for collecting the sorted corresponding particle samples, the three collecting bins 7 are respectively in one-to-one correspondence with the three branch circuits, and each collecting bin 7 is respectively arranged at the downstream of the corresponding branch circuit and is communicated with the corresponding branch circuit; wherein the first air passage 211 is communicated with the particle chamber 5, and the second air passage 212 is communicated with the sheath fluid chamber 6; so set up, simple structure, each bin arranges rationally.
In this embodiment, since the particle sample is set as a cell, the particle chamber 5 is correspondingly set as a cell dissociation chamber 51.
Referring to fig. 6, in the present invention, the sorting flow of the microparticle sorting apparatus includes the following steps:
s10: controlling the driving device to be started to drive the particle sample to flow;
s20: controlling a detection device to be opened to detect the particle sample flowing through so as to identify parameter information of the particle sample, wherein the parameter information comprises the size of the particle sample and the content of a target substance in the particle sample;
s30: and controlling the sorting device to drive the particle sample to move along the radial direction of the inlet section according to the parameter information so as to enable the particle sample to flow into the corresponding outlet section.
In this embodiment, during the sorting process, when the particle sample flows through the inlet section, the detection device detects the particle sample and identifies parameter information of the particle sample (i.e. the size of the particle sample and the content of the target substance in the particle sample), and after the controller obtains the parameter information of the particle sample, the controller controls the sorting device to drive the particle sample to move so as to flow to the corresponding outlet section; according to the arrangement, the microparticle sorting device is simple in structure and concise in sorting steps, and through the combined action of the detection device and the sorting device, the particle sample sorting effect is good, repeated sorting is not needed, and the target cell loss probability is reduced.
Referring to fig. 7, specifically, the outlet sections include three branches arranged side by side, an included angle is formed between two adjacent branches, the three branches include a first branch, a second branch and a third branch, the first branch and the third branch extend from two opposite sides of the inlet section respectively toward directions away from each other, a first air inlet is disposed at one side of the inlet section close to the first branch, and a second air inlet is disposed at one side of the inlet section close to the third branch; the sorting device comprises a sorting air source, a first valve and a second valve, wherein the first valve and the second valve are respectively arranged on the first air inlet and the second air inlet; wherein the controller is electrically connected to the first valve, the second valve and the detecting device, and the step S30 of controlling the sorting device to drive the particle sample to move along the radial direction of the inlet section according to the parameter information so as to flow the particle sample into the corresponding outlet section includes:
s301: the parameter information is used for judging the type of the particle sample, wherein the type of the particle sample comprises standard particles, excess particles and deficient particles;
s302: when the particle sample is a standard particle, the first valve and the second valve are both closed, so that the standard particle flows into the second branch;
s303: when the particle sample is an excess particle, the first valve is closed, and the second valve is opened, so that the sorting gas source is communicated with the inlet section through the second gas inlet, and the excess particle is driven to move to one side close to the first branch along the radial direction of the inlet section and flow to the first branch;
s304: when the particle sample is an insufficient particle, the second valve is closed, and the first valve is opened, so that the sorting gas source is communicated with the inlet section through the first gas inlet, and the excessive particle is driven to move to a side close to the third branch along the radial direction of the inlet section and flow to the third branch.
In this embodiment, after the particle sample flows through the detection device, the detection device identifies parameter information of the particle sample, and the controller controls the first valve and the second valve to selectively open according to the parameter information of the particle sample, so that the gas source is selectively communicated with the inlet section through the first gas inlet and the second gas inlet, and the particle sample can flow into a corresponding branch under the pushing of the gas flow, thereby realizing the sorting of the particle sample.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (10)

1. A microparticle sorting device, comprising:
the base body is provided with a sample flow channel for the flow of the particle sample, the sample flow channel is arranged in a sealing manner, the sample flow channel comprises an inlet section and a plurality of outlet sections, and the outlet sections are arranged in different directions;
a drive means for driving the particle sample to flow along the sample flow path;
the detection device is arranged in the midstream of the inlet section and is used for detecting the particle sample flowing through the detection device so as to identify parameter information of the particle sample, wherein the parameter information comprises the size of the particle sample and the content of a target substance in the particle sample;
the sorting device is arranged at the downstream of the inlet section and is used for driving the particle samples to move along the radial direction of the inlet section so as to adjust the flow direction of the particle samples, so that the particle samples can flow to the corresponding outlet section; and (c) a second step of,
and the control device comprises a controller which is electrically connected with the detection device and the sorting device.
2. The particulate sorting apparatus of claim 1, wherein the inlet section comprises:
a particle segment for flow of the particle sample;
the two sheath liquid sections are used for flowing sheath liquid, the two sheath liquid sections and the particle section are arranged side by side and are respectively arranged at two sides of the particle section, and the part of each sheath liquid section close to the outlet of the sheath liquid section and the particle section form an included angle; and the number of the first and second groups,
a confluence section, the inlets of which are respectively connected with the particle sections and the outlets of the two sheath liquid sections, the outlets of which are respectively connected with the outlet sections, wherein the confluence section is used for flowing the particle sample coated with the sheath liquid;
the detection device is arranged in the midstream of the confluence section;
the sorting device is arranged at the downstream of the confluence section.
3. The microparticle sorting device according to claim 2, wherein the driving device includes a gas supply device, and the gas supply device includes:
a driving gas source;
a first air channel, an inlet of the first air channel is connected with the driving air source, and an outlet of the first air channel is communicated with the upstream of the particle section, so that when the driving air source is opened, the particle sample can be driven to flow along the particle section; and (c) a second step of,
and the inlet of the second air passage is connected with the driving air source, and the outlet of the second air passage is respectively communicated with the two sheath liquid sections, so that when the driving air source is opened, the sheath liquid can be driven to flow.
4. The microparticle sorting device according to claim 1, wherein the outlet sections include three branches arranged side by side, two adjacent branches are arranged at an angle, the three branches include a first branch, a second branch and a third branch, and the first branch and the third branch extend from two opposite sides of the inlet section toward directions away from each other.
5. The microparticle sorting device as claimed in claim 4, wherein the inlet section has a diameter D, and the first branch has a diameter D 1 And satisfies the following relationship: d 1 ≦D≦2D 1 (ii) a And/or the presence of a gas in the gas,
the diameter of the inlet section is D, and the diameter of the second branch is D 2 And satisfies the following relationship: d 2 ≦D≦2D 2 (ii) a And/or the presence of a gas in the atmosphere,
the diameter of the inlet section is D, and the diameter of the third branch is D 3 And satisfies the following relationship: d 3 ≦D≦2D 3 。
6. The microparticle sorting device according to claim 4, wherein the inlet section has a first guiding groove formed on an inner wall thereof adjacent to the first branch, and a bottom wall of the first guiding groove has an arc-shaped guiding surface; and/or the presence of a gas in the gas,
the inlet section is provided with a second diversion trench close to the inner wall of the third branch, and the bottom wall of the second diversion trench is provided with an arc diversion surface.
7. The microparticle sorting device according to claim 4, wherein the inlet section is provided with a first air inlet on a side close to the first branch and a second air inlet on a side close to the third branch;
the sorting device comprises a sorting air source, a first valve and a second valve, wherein the first valve and the second valve are respectively and correspondingly arranged on the first air inlet and the second air inlet;
the controller is electrically connected with the first valve, the second valve and the detection device, so as to control the first valve and the second valve to be selectively opened after the detection device identifies the parameter information of the particle sample, so that the first air inlet and the second air inlet are correspondingly communicated with the air source.
8. The microparticle sorting device according to claim 4, wherein the seat further has a magnetized flow path formed thereon, the magnetized flow path being used for flowing a magnetized reagent, and the magnetized flow path being located upstream of the inlet section and communicating with the inlet section for allowing the magnetized reagent to cover the particle sample;
the sorting device comprises a magnetic suction assembly, and the magnetic suction assembly comprises a first electromagnet and a second electromagnet which are respectively arranged on two sides of the inlet section;
the controller is electrically connected with the first electromagnet, the second electromagnet and the detection device, so that after the detection device identifies the parameter information of the particle sample, the coil of the first electromagnet and the coil of the second electromagnet are controlled to be selectively electrified, and magnetic fields are respectively and correspondingly and selectively generated on two sides of the inlet section.
9. The microparticle sorting device according to claim 1, wherein the detection device includes an optical detection unit, and the optical detection unit includes:
the optical detector is arranged on the base and used for detecting the size of the particle sample and the content of the target substance in the particle sample; and (c) a second step of,
an analyzer in electrical communication with the optical detector to identify a type of the particle sample;
wherein the controller is electrically connected to the analyzer and the sorting device.
10. The microparticle sorting device according to claim 9, wherein the optical detector includes:
the light source device is arranged above the base body, and the light emitting end of the light source device faces the inlet section and is used for irradiating the particle sample flowing through so as to enable the particle sample to generate scattered light and fluorescence;
a first detector for receiving the scattered light to detect a size of the particle sample;
a second detector for receiving the fluorescence to detect the content of the target substance in the particle sample;
wherein the analyzer is electrically connected to the first detector and the second detector.
CN202210859824.9A 2022-07-21 2022-07-21 Microparticle sorting device Pending CN115193728A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210859824.9A CN115193728A (en) 2022-07-21 2022-07-21 Microparticle sorting device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210859824.9A CN115193728A (en) 2022-07-21 2022-07-21 Microparticle sorting device

Publications (1)

Publication Number Publication Date
CN115193728A true CN115193728A (en) 2022-10-18

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210859824.9A Pending CN115193728A (en) 2022-07-21 2022-07-21 Microparticle sorting device

Country Status (1)

Country Link
CN (1) CN115193728A (en)

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