CN115191514A - Method for reducing cholesterol in duck egg yolk and improving nutrition of fatty acid and application - Google Patents
Method for reducing cholesterol in duck egg yolk and improving nutrition of fatty acid and application Download PDFInfo
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- CN115191514A CN115191514A CN202210993774.3A CN202210993774A CN115191514A CN 115191514 A CN115191514 A CN 115191514A CN 202210993774 A CN202210993774 A CN 202210993774A CN 115191514 A CN115191514 A CN 115191514A
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Abstract
The invention discloses a method for reducing cholesterol content of duck egg yolk and improving fatty acid nutrition and application thereof. The invention establishes a regression model of the addition level (independent variable) of the eucommia leaf powder and the content (dependent variable) of the cholesterol in the egg yolk for estimating the minimum addition, and the regression model not only shows the significant relation between the independent variable and the dependent variable; and the influence strength of independent variables on dependent variables is shown, and meanwhile, the method is applied to a feed formula to obtain a corresponding feed formula so as to improve the nutritional quality of duck eggs and the scientific and technological additional value of laying duck breeding.
Description
Technical Field
The invention relates to feed, belongs to the technical field of laying duck feed, and particularly relates to a feed formula for reducing cholesterol in duck egg yolk and improving the nutrition of egg yolk fatty acid
Background
Eucommia ulmoides (Eucomia ulmoides Oliv) is a special Chinese medicinal material, belongs to deciduous trees, and leaves of the Eucommia ulmoides are renewable and rich in resources. The eucommia leaves contain medicinal active ingredients such as chlorogenic acid, polyphenol compounds, flavonoid compounds, rutin, quercetin, geniposide, aucubin, geniposide, asperuloside, lignan and the like, and have the effects of protecting liver, promoting urination, preventing cerebral hemorrhage, delaying senescence, reducing blood pressure and the like.
In recent years, the dietary structure of human health has advocated low-fat, low-saturated fatty acids and low-cholesterol food sources, as well as high-polyunsaturated fatty acid intake. Polyunsaturated fatty acid, as an essential fatty acid, plays an important role in maintaining the normal growth and development of the body and preventing various metabolic diseases, such as: coronary artery disease, hypertension, diabetes, cancer, inflammatory autoimmune disease. Polyunsaturated fatty acids include n-3 and n-6 polyunsaturated fatty acids, n-3 polyunsaturated fatty acids include α -C18:3n-3 (ALA), C20:5n-3 (EPA), C22:6n-3 (DHA), a common edible vegetable oil, such as sunflower oil, corn oil, soybean oil, contains higher n-6 polyunsaturated fatty acids, but lower n-3 polyunsaturated fatty acids, and therefore, how to increase the proportion of n-3 polyunsaturated fatty acids in the human dietary structure is one of the key points of current human nutritional attention. The poultry egg yolk is the most nutritious food and is rich in a large amount of saturated fatty acid, unsaturated fatty acid, phospholipid, vitamin, amino acid, cholesterol and the like, and the nutritional composition of the poultry egg yolk is mainly influenced by daily ration factors besides genetic factors, so that the content of cholesterol and saturated fatty acid in eggs is reduced through daily ration nutrition regulation, the content of polyunsaturated fatty acid is increased, particularly the content of n-3 polyunsaturated fatty acid, and the poultry egg yolk can provide a healthier food for human beings.
Disclosure of Invention
The invention aims to provide a method for reducing the cholesterol of duck egg yolk and improving the nutrition of the yolk fatty acid and application thereof, and improve the nutritional quality of duck eggs and the scientific and technological added value of laying duck breeding.
In order to achieve the purpose, the invention adopts the following scheme: a method for reducing cholesterol in duck egg yolk and improving fatty acid nutrition in the duck egg yolk and an application thereof comprise the following steps:
(1) The method comprises the steps of taking corn-soybean meal which is a common feed raw material as basic daily ration, wherein the average level of nutrients of the basic daily ration reaches the recommended amount of meat duck feeding standard, adding eucommia leaf powder at different levels on the basis of the basic daily ration, wherein the adding levels are 0%, 1%, 2%, 4% and 6%, and totally 5 treatment groups are provided, wherein the 0 adding group is a control group. The added eucommia ulmoides leaf powder contains 9.33% of water, 17.18% of crude protein, 13.48% of crude fat, 22.05% of crude fiber, 7.97% of ash, 29.98% of nitrogen-free extract, 1.88% of Ca, 0.19% of P, 72.36mg/g of total phenol, 13.41mg/g of total flavone and 22.68mg/g of chlorogenic acid.
(2) All the raw materials are crushed and mixed uniformly, 5 percent of water is added, the particle size of the granules is not more than 3mm, and the granules are dried in the dark for standby;
(3) Adopting single-factor completely random experimental design, selecting 480 healthy 32-week-old egg-laying Sansui ducks, and randomly dividing the three-ear ducks into 5 treatments, wherein each treatment is 8 repetitions, and each repetition is 12 ducks; feeding complete feed with different addition levels respectively in different treatments, wherein the feeding period is 7 weeks, and feeding daily ration with different addition levels of eucommia respectively in different treatments; wherein the pre-feeding period is 1 week.
(4) In the formal test period, the egg laying number, the egg weight (g), the soft shell breaking and malformed egg number and the dead duck number of each group (taking the repetition as a unit) are recorded every day, and the feed intake of each week is counted. Finally, the total egg weight (kg), laying rate (%), average egg weight (g), average daily feed intake (g/d/egg), feed-to-egg ratio (g/g), average daily laying rate (g/egg), qualified egg rate (%), and mortality (%) during the test period were calculated.
(5) On the day of test completion, 2 test ducks close to the average weight of each cage are selected from each cage, the jugular vein is subjected to blood sampling for 2ml, the test ducks are placed in heparin sodium anticoagulated blood sampling tubes, blood plasma is prepared by centrifugation at 4 ℃ and 3000RPM for 10min, and the test ducks are frozen and stored at-20 ℃ for later use and used for measuring the subsequent biochemical indexes of the blood plasma, wherein the measurement indexes comprise alanine Aminotransferase (ALT), glutamic acid transaminase (AST), total Bile Acid (TBA), total Protein (TP), albumin (ALB), glucose (GLU), uric Acid (UA), cholesterol (Chol), triglyceride (TG), high-density lipoprotein cholesterol (HDL) and low-density lipoprotein cholesterol (LDL).
(6) Randomly selecting 2 eggs from each repetition for conventional egg quality analysis one day before the test is finished, randomly selecting 16 eggs in each group after all eggs are repeatedly collected, wherein the eggs are 80 eggs in total, marking the groups and bringing the eggs back to a laboratory on the day of collection for egg quality determination, and the determination indexes comprise: egg weight, yolk ratio, egg specific gravity, egg shape index, eggshell thickness, egg white height, yolk color, haugh unit, and eggshell strength.
(7) And after the quality of the eggs is measured, collecting the egg yolks, freeze-drying the egg yolks into powder, calculating the proportion of dry matter of the egg yolks, and using the freeze-dried egg yolks for measuring the content of crude egg yolk, total fat, total phenols, total flavonoids, chlorogenic acid, triglyceride, total cholesterol and fatty acid.
(8) And calculating the minimum addition amount of the eucommia leaf powder according to a linear broken line regression model (y = l + u (r-x)) by taking the addition amount ratio of the eucommia leaf powder in the daily ration as an independent variable x and the cholesterol content in the egg yolk as a dependent variable y.
Wherein: u represents the slope of the curve; l is the inflection point at r = x; r represents the minimum cholesterol content.
In the method for estimating the lowest addition amount of the eucommia ulmoides leaf powder, the test laying duck is a 32-week-old Sansui duck.
In the method for estimating the lowest addition amount of the folium cortex eucommiae powder, the preparation method of the compound feed with different levels of the folium cortex eucommiae powder content comprises the following steps: firstly, preparing basic ration, wherein the basic ration comprises the following formula: 62.00 percent of corn, 1.50 percent of bran, 26.10 percent of soybean meal, 0.30 percent of salt, 1.50 percent of calcium hydrophosphate, 7.48 percent of stone powder, 1.00 percent of premix and 0.12 percent of DL-methionine. Then 0, 1%, 2%, 4%, and 6% of folium Eucommiae powder was added to the basal diet, respectively.
In particular, the invention relates to a method for reducing cholesterol and increasing fatty acid nutrition of duck egg yolk, which is used for preparing feed for any one or more of the following purposes: reducing cholesterol content in yolk without affecting egg laying performance and egg quality, regulating fat metabolism, promoting deposition of active substances of yolk and eucommia ulmoides, and improving fatty acid nutrition of yolk.
Preferably, after the feeding test is finished, the lowest addition amount of the eucommia ulmoides leaf powder is calculated according to a linear broken line regression model (y = l + u x (r-x)) by taking the addition amount ratio of the eucommia ulmoides leaf powder in daily ration as an independent variable x and the cholesterol content in egg yolk as a variable y, and the inflection point of the addition amount of the eucommia ulmoides leaf powder is 1.4%, namely: when the addition amount of the eucommia ulmoides leaf powder is higher than 1.4%, the content of cholesterol in the egg yolk is reduced in a plateau stage, and the egg laying performance and the egg quality are not obviously changed. The corresponding feed formula is as follows: 62.00 percent of corn, 1.50 percent of bran, 26.10 percent of soybean meal, 0.30 percent of salt, 1.50 percent of calcium hydrophosphate, 7.48 percent of stone powder, 1.00 percent of premix, 0.12 percent of DL-methionine and at least 1.4 percent of eucommia leaf powder.
Preferably, after a duck feed capable of regulating fat metabolism is fed, plasma is collected and glutamic pyruvic transaminase (ALT), glutamic oxalacetic transaminase (AST), total Bile Acid (TBA), total Protein (TP), albumin (ALB), glucose (GLU), uric Acid (UA), cholesterol (Chol), triglyceride (TG), high-density lipoprotein cholesterol (HDL) and low-density lipoprotein cholesterol (LDL) are measured, and as a result, the addition of the eucommia leaf powder is found to remarkably influence the total plasma protein (TP), the high-density lipoprotein cholesterol (HDL) and the low-density lipoprotein cholesterol (LDL) and has no remarkable influence on ALT, AST, TBA, ALB, GLU, UA, CHOL and TG. With the addition of the additive, the total plasma protein TP and the high-density lipoprotein cholesterol HDL are increased linearly, and the low-density lipoprotein cholesterol LDL is reduced in a secondary curve. The corresponding feed formula is as follows: 62.00 percent of corn, 1.50 percent of bran, 26.10 percent of soybean meal, 0.30 percent of salt, 1.50 percent of calcium hydrophosphate, 7.48 percent of stone powder, 1.00 percent of premix, 0.12 percent of DL-methionine and at least 2 percent of eucommia leaf powder.
Preferably, the duck feed capable of improving the deposition of chlorogenic acid in egg yolk is characterized in that after a feeding test is finished, 2 eggs are randomly selected from each repetition for nutritional active matter analysis, egg yolk is collected and freeze-dried into powder, the dry matter ratio of the egg yolk is calculated, and the freeze-dried egg yolk is used for measuring the crude egg yolk to total fat ratio, the total phenols, the total flavonoids, the chlorogenic acid and the triglyceride content of the egg yolk. The results show that: the addition of the eucommia ulmoides leaf powder to the daily ration has no obvious influence on dry egg yolk substances, crude egg white, total fat, total phenols, total flavonoids and triglyceride, but the content of the chlorogenic acid in the egg yolk is obviously influenced, and the content of the chlorogenic acid in the egg yolk is linearly increased along with the gradual increase of the addition amount of the eucommia ulmoides leaf powder. The corresponding feed formula is as follows: 62.00 percent of corn, 1.50 percent of bran, 26.10 percent of soybean meal, 0.30 percent of salt, 1.50 percent of calcium hydrophosphate, 7.48 percent of stone powder, 1.00 percent of premix, 0.12 percent of DL-methionine and at least 4 percent of eucommia leaf powder.
Preferably, a duck feed capable of improving the fatty acid nutrition of duck egg yolk, after the feeding test is finished, randomly selecting 2 eggs from each repetition for fatty acid analysis, collecting the egg yolk and freeze-drying the egg yolk into powder, wherein the freeze-dried egg yolk is used for determining the content of fatty acid, and the result shows that: the addition of the eucommia ulmoides leaf powder to the daily ration obviously influences the content of total Saturated Fatty Acid (SFA) in the yolk, wherein the content comprises C14: 0. c16:0 and C18:0, and as the addition amount of the eucommia ulmoides leaf powder increases, the total SFA, C14: 0. c16:0 and C18:0 is linearly decreased; the addition of the eucommia leaf powder in the daily ration obviously improves the total monounsaturated fatty acid (MUFA) and C18:1n =9 content and varies significantly linearly. The addition of the eucommia ulmoides leaf powder of the daily ration has no obvious influence on the content of total PUFA and n-6PUFA, but the n-3PUFA is obviously influenced, and the n-3PUFA in the egg yolk is linearly increased along with the increase of the addition amount of the eucommia ulmoides leaf powder, wherein C22:5n-3 (DPA) and C22:5n-3 (DHA) are obviously linearly increased, and EPA is obviously linearly decreased. The ratio of n-6 to n-3 in PUFA (n-6: n-3) and the ratio of PUFA to SFA (PUFA: SFA) are also important nutritional indicators, and it was found in this experiment that the addition of eucommia ulmoides leaf powder to a diet significantly affects the ratio of n-6: SFA, and as the addition amount increases, the ratio of n-6: n-3 decreases linearly, whereas the PUFA SFA increases linearly. The corresponding feed formula is as follows: 62.00 percent of corn, 1.50 percent of bran, 26.10 percent of soybean meal, 0.30 percent of salt, 1.50 percent of calcium hydrophosphate, 7.48 percent of stone powder, 1.00 percent of premix, 0.12 percent of DL-methionine and at least 1 percent of eucommia leaf powder.
The invention has the beneficial effects that:
(1) According to the invention, through single-factor random experimental design, the influence of the addition of the eucommia ulmoides leaf powder in the daily ration of the laying duck on the nutrient cholesterol and fatty acid composition of egg yolk is researched by adopting a feeding test, and the result proves that the addition of the eucommia ulmoides leaf powder can effectively reduce the cholesterol content of the duck egg and improve the nutrient value of the fatty acid of the egg yolk.
(2) The minimum addition amount is estimated by establishing a regression model of the addition level (independent variable) of the eucommia leaf powder and the content (dependent variable) of cholesterol in egg yolk, and the regression model not only shows the significant relation between the independent variable and the dependent variable; and the influence strength of the independent variable on the dependent variable is shown, so that the method can obtain more accurate test results.
Drawings
FIG. 1 is a flow chart of the experimental design of the present invention.
Detailed Description
Example 1: the influence of adding eucommia leaf powder (0, 1%, 2%, 4%, 6%) with different concentrations in the daily ration of laying ducks on the egg laying performance of the three-ear ducks of 33-39 weeks age is researched by adopting a single-factor completely random experimental design and adopting a corn-soybean meal type basic feed, and the result is shown in table 1: the addition of the eucommia leaf powder in the daily ration has no significant influence on the total egg weight, the laying rate, the average egg weight, the average daily egg weight, the feed-egg ratio, the death and culling rate and the qualified egg rate of the Sansui duck (P is more than 0.05). The average daily feed intake (P is less than 0.05) of the Sansui duck is obviously influenced by adding the eucommia ulmoides leaf powder into the daily ration, and the average daily feed intake is in a linear rising trend (P is less than 0.05) along with the increase of the addition amount.
Table 1. Influence of different levels of eucommia ulmoides leaf powder addition on egg laying performance of Sansui ducks of 33-39W
Example 2: the influence of the addition of eucommia leaf powder (0, 1%, 2%, 4%, 6%) with different concentrations on biochemical indicators of the plasma of the Sansui ducks aged 33-39 weeks is researched by adopting a single-factor completely random experimental design and adopting a corn-soybean meal type basic feed, and the results are shown in Table 2: the addition of the eucommia ulmoides leaf powder obviously influences total plasma protein (TP), high-density lipoprotein cholesterol (HDL) and low-density lipoprotein cholesterol (LDL) (P is less than 0.05), and has no obvious influence on ALT, AST, TBA, ALB, GLU, UA, CHOL and TG (P is more than 0.05). With the addition of the additive, the total plasma protein TP and the high-density lipoprotein cholesterol HDL are increased linearly (P < 0.05), and the low-density lipoprotein cholesterol LDL is decreased in a secondary curve (P < 0.05).
Table 2. Influence of different levels of folium Eucommiae powder on biochemical indexes of blood plasma of Sansui duck of 33W-39W
Example 3: the influence of the addition of eucommia leaf powder (0, 1%, 2%, 4%, 6%) with different concentrations on the quality of the eggs of the three-ear ducks aged 33-39 weeks is researched by adopting a single-factor completely random experimental design and adopting a corn-soybean meal type basic feed, and the result is shown in table 3: the addition of the daily ration eucommia leaf powder has no obvious influence on each index of the quality of the three-ear duck eggs of 33W to 39W (P is more than 0.05).
Table 3. Influence of different levels of eucommia ulmoides leaf powder addition on quality of three-ear duck eggs of 33W-39W
Example 4: the influence of the addition of eucommia leaf powder (0, 1%, 2%, 4%, 6%) with different concentrations on the nutritional ingredients of the yolk of the Sansui duck aged 33-39 weeks is studied by using a single-factor completely random experimental design and a corn-soybean meal type basic feed, and the results are shown in Table 4: the addition of the eucommia ulmoides leaf powder to the daily ration has no obvious influence (P is more than 0.05) on dry matters, crude egg white, total fat, total phenols, total flavonoids and triglyceride of egg yolk, but has obvious influence on the content of chlorogenic acid and total cholesterol of the egg yolk (P is less than 0.05), and the content of the chlorogenic acid of the egg yolk is linearly increased (P is less than 0.05) and the content of the total cholesterol of the egg yolk is linearly reduced (P is less than 0.05) along with the gradual increase of the addition amount of the eucommia ulmoides leaf powder. Fitting the regression relationship between the addition level of the daily ration eucommia leaf powder and the content of the yolk cholesterol by using a linear broken line model, wherein the result shows that: when the addition amount of the eucommia ulmoides leaf powder is 1.4%, the curve reaches the lowest inflection point, and the inflection point is 13.3797 (mg/g). The fitted curve is y =13.3797+1.619 (1.4048-x), (P =0.0449 2 ＝0.953)。
TABLE 4 influence of folium Eucommiae powder added with Sansui duck egg yolk nutrient component (fresh weight basis)
Example 5: the influence of the addition of eucommia leaf powder (0, 1%, 2%, 4%, 6%) with different concentrations on the nutritional ingredients of the yolk of the Sansui duck aged 33-39 weeks is studied by using a single-factor completely random experimental design and a corn-soybean meal type basic feed, and the results are shown in Table 5: the addition of the eucommia ulmoides leaf powder to the daily ration obviously influences the content of total Saturated Fatty Acid (SFA) in the yolk, wherein the content comprises C14: 0. c16:0 and C18:0 (P < 0.05), and as the addition amount of the eucommia ulmoides leaf powder increases, the total SFA, C14: 0. c16:0 and C18:0 is linearly reduced (P is less than 0.05); the addition of the eucommia leaf powder into the daily ration obviously improves the total monounsaturated fatty acid (MUFA) and C18:1n =9 (P < 0.05) and varies significantly linearly. The addition of the eucommia ulmoides leaf powder of the daily ration has no significant influence on the content of total PUFA and n-6PUFA (P is more than 0.05), but n-3PUFA is significantly influenced, and the n-3PUFA in egg yolk is linearly increased (P is less than 0.05) along with the increase of the addition amount of the eucommia ulmoides leaf powder, wherein C22:5n-3 (DPA) and C22:5n-3 (DHA) are significantly linearly increased (P is less than 0.05), and EPA is significantly linearly decreased (P is less than 0.05). The ratio of n-6 to n-3 in PUFA (n-6: n-3) and the ratio of PUFA to SFA (PUFA: SFA) are also important nutritional indicators, and it was found in this experiment that the addition of eucommia ulmoides leaf powder to a diet significantly affects the ratio of n-6: n-3 and PUFA SFA (P < 0.05), and as the addition amount increases, the ratio of n-6: n-3 decreased linearly (P < 0.05) and PUFA: SFA increased linearly (P < 0.05).
Table 5. Influence of different levels of eucommia ulmoides leaf powder addition on yolk fatty acid content of Sansui duck from 33W to 39W
Claims (8)
1. A method for reducing cholesterol in duck egg yolk and improving nutrition of fatty acid is characterized by comprising the following steps:
(1) Preparing eucommia leaf powder raw materials, namely picking fresh eucommia leaves, cleaning the fresh eucommia leaves with clear water, filtering the water, cutting the water into thin strips, carrying out enzyme deactivation for 10 seconds at the steam temperature of 100 ℃ after preparing eucommia leaf strips, spreading the eucommia leaf strips subjected to enzyme deactivation, drying the eucommia leaf strips in the shade, grinding the eucommia leaf strips into powder by using a grinder, and measuring conventional nutritional ingredients of the eucommia leaf powder for later use;
(2) Common feed raw materials of corn and soybean meal are taken as basic daily ration, the average level of nutrient content of the basic daily ration reaches the recommended amount of meat duck feeding standard, eucommia leaf powder with different levels is added on the basis of the basic daily ration, the addition levels are 0, 1%, 2%, 4% and 6%, wherein 0 addition group is a control group;
(3) All the raw materials are crushed and mixed uniformly, 5 percent of water is added, the particle size of the granules is not more than 3mm, and the granules are dried in the dark for standby;
(4) Adopting single-factor completely random experimental design, selecting 480 healthy 32-week-old egg-laying Sansui ducks, and randomly dividing the three ducks into 5 treatments, wherein the addition levels of the eucommia ulmoides leaf powder are 0, 1%, 2%, 4% and 6%, each treatment is repeated for 8 times, and each treatment is repeated for 12 ducks; feeding test daily feed with different addition levels in different treatments, wherein the feeding period is 7 weeks, and the pre-feeding period is 1 week;
(5) In the formal test stage, the number of eggs laid, the weight (g) of the eggs, the number of soft-shelled and malformed eggs and the number of dead ducks are recorded every day, and the feed intake per week is counted; finally, calculating the total egg weight (kg), the laying rate (%), the average egg weight (g), the average daily feed intake (g/d/egg), the feed-egg ratio (g/g), the average daily laying rate (g/egg), the qualified egg rate (%) and the mortality and elimination rate (%);
(6) On the day of test completion, selecting 2 test ducks with average weight close to the cages per cage, collecting blood in jugular vein by 2ml, placing the test ducks in heparin sodium anticoagulated blood collection tubes, centrifuging at 4 ℃ and 3000RPM for 10min to prepare blood plasma, freezing and storing at-20 ℃ for later use, and measuring the subsequent plasma biochemical indexes, wherein the measuring indexes comprise glutamic pyruvic transaminase (ALT), glutamic oxalacetic transaminase (AST), total Bile Acid (TBA), total Protein (TP), albumin (ALB), glucose (GLU), uric Acid (UA), cholesterol (Chol), triglyceride (TG), high density lipoprotein cholesterol (HDL) and low density lipoprotein cholesterol (LDL);
(7) Randomly selecting 2 eggs from each repetition for conventional egg quality analysis one day before the test is finished, randomly selecting 16 eggs in each group after all eggs are repeatedly collected, wherein the eggs are 80 eggs in total, marking the groups and bringing the eggs back to a laboratory on the day of collection for egg quality determination, and the determination indexes comprise: egg weight, yolk ratio, egg specific gravity, egg shape index, eggshell thickness, egg white height, yolk color, hawski unit and eggshell strength;
(8) After the egg quality measurement is finished, collecting yolk, freeze-drying the yolk into powder, calculating the dry matter proportion of the yolk, and using the freeze-dried yolk to measure the content of crude egg yolk, total fat, total phenols, total flavonoids, chlorogenic acid, triglyceride, total cholesterol and fatty acid;
(9) And calculating the lowest addition amount of the eucommia leaf powder according to a linear broken line regression model [ y = l + u (r-x) ] by taking the addition level of the eucommia leaf powder in the daily ration as an independent variable x and the cholesterol content in the egg yolk as a dependent variable y.
2. The method for reducing cholesterol in duck egg yolk and improving fatty acid nutrition of claim 1, wherein the eucommia ulmoides leaf powder contains 9.33% of water, 17.18% of crude protein, 13.48% of crude fat, 22.05% of crude fiber, 7.97% of ash, 29.98% of nitrogen-free extract, 1.88% of Ca, 0.19% of P, 72.36mg/g of total phenols, 13.41mg/g of total flavonoids and 22.68mg/g of chlorogenic acid.
3. The method for reducing cholesterol in duck egg yolk and improving nutrition of fatty acid as claimed in claim 1 or 2, wherein the compound feed with different content of folium Eucommiae powder is prepared by: firstly, preparing basic ration, wherein the basic ration comprises the following formula: 62.00 percent of corn, 1.50 percent of bran, 26.10 percent of soybean meal, 0.30 percent of salt, 1.50 percent of calcium hydrophosphate, 7.48 percent of stone powder, 1.00 percent of premix and 0.12 percent of DL-methionine; then 0, 1%, 2%, 4%, and 6% of folium Eucommiae powder was added to the basal diet, respectively.
4. Applying the method of claim 1 to a feed formulation of any one or more of the following genera: reducing cholesterol content in yolk without affecting egg laying performance and egg quality, regulating fat metabolism, promoting deposition of active substances of yolk and eucommia ulmoides, and improving fatty acid nutrition of yolk.
5. A duck feed capable of reducing the cholesterol content of egg yolk without affecting egg laying performance and egg quality, characterized by the use of the basal ration formulation of claim 3: 62.00 percent of corn, 1.50 percent of bran, 26.10 percent of soybean meal, 0.30 percent of salt, 1.50 percent of calcium hydrophosphate, 7.48 percent of stone powder, 1.00 percent of premix and 0.12 percent of DL-methionine; then at least 1.4% of folium Eucommiae powder is added into the basic daily ration.
6. A duck feed capable of regulating fat metabolism, characterized in that the basic ration formula of claim 3 is used: 62.00 percent of corn, 1.50 percent of bran, 26.10 percent of soybean meal, 0.30 percent of salt, 1.50 percent of calcium hydrophosphate, 7.48 percent of stone powder, 1.00 percent of premix and 0.12 percent of DL-methionine; then at least 2% of folium Eucommiae powder is added into the basic daily ration.
7. A duck feed capable of improving the deposition of chlorogenic acid in egg yolk, which is characterized in that the basic ration formula of claim 3 is adopted: 62.00 percent of corn, 1.50 percent of bran, 26.10 percent of soybean meal, 0.30 percent of salt, 1.50 percent of calcium hydrophosphate, 7.48 percent of stone powder, 1.00 percent of premix and 0.12 percent of DL-methionine; then at least 4% of folium Eucommiae powder is added into the basic daily ration.
8. A duck feed for improving the fatty acid nutrition of duck egg yolk, characterized in that the basic ration formula of claim 3 is used: 62.00 percent of corn, 1.50 percent of bran, 26.10 percent of soybean meal, 0.30 percent of salt, 1.50 percent of calcium hydrophosphate, 7.48 percent of stone powder, 1.00 percent of premix and 0.12 percent of DL-methionine; then at least 1% of folium Eucommiae powder is added into the basic daily ration.
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| 周岩等: "杜仲叶粉对蛋鸡血清生化指标和蛋黄胆固醇含量的影响", 湖北畜牧兽医, no. 2, pages 52 - 53 * |
| 康克浪等: "杜仲叶对蛋鸡生产性能、蛋品质和经济效益的影响", 中国家禽, vol. 40, pages 20 - 24 * |
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