CN115137820B - Preparation method of nano enzyme complex for treating tumors - Google Patents
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Abstract
The invention discloses a preparation method of a nano enzyme compound for treating tumors, which comprises the following steps: ball milling the CNT and the steel ball; dispersing the ball-milled CNTs; sequentially taking urea, adding sodium dodecyl sulfate into a beaker, and adding a dispersed CNT solution; dissolving ferric sulfate in water, and adding the solution; dissolving phosphoric acid in water, and adding the solution; heating, cooling and suction filtering the mixed solution to obtain FePOs and CNT composite; introducing H2 into the FePOs and CNT composite, and calcining at high temperature to obtain the FeP and CNT composite; weighing FeP, CNT composite and ionic surfactant, grinding; dissolving another ionic surfactant in water, adding a ground sample, dispersing, and standing to obtain an upper dispersion; dissolving the prepared GO liquid in water and dispersing; conveying the CNT dispersion liquid to the GO liquid, and carrying out electrostatic self-assembly; ammonium bicarbonate is dissolved in water, and is added into the mixed solution, and suction filtration is carried out after electrostatic self-assembly to obtain FeP, CNT, GO compound.
Description
Technical Field
The invention relates to the technical field of nano biology, in particular to a preparation method of a nano enzyme complex for treating tumors.
Background
With the advent of nano-enzymes with enzyme-like activity, enzyme therapy anti-tumor will have an opportunity to make breakthrough progress in practical application, nano-enzymes with peroxidase activity, catalase activity, oxidase activity and superoxide dismutase activity are widely used in the field of tumor treatment, in which conventional physical means can also play an effective killing role on tumor cells, but side effects are large, and researches show that apoptosis of tumor cells can be induced by relatively mild stimulation (acoustic, optical, electric, magnetic, etc.).
However, the current research on tumor treatment by nano-enzymes is relatively independent, few researches are conducted to explore the anti-tumor effect of nano-enzymes under the condition of external stimulation, carbon Nanotubes (CNT) and Graphene (GO) are nano-materials with enzyme-like activity, and have certain electric property, magnetic property and photo-thermal property, and although the carbon-based nano-enzymes have certain effect on anti-tumor aspect, the application of the carbon-based nano-enzymes in tumor treatment is limited because the enzyme activity of the carbon-based nano-enzymes is not high, and the iron-based nano-enzymes generally have higher enzyme-like activity and have better effect on tumor treatment, so that the combination of the iron-based nano-materials and the carbon-based nano-materials can enhance the enzyme activity and respond to external stimulation.
Disclosure of Invention
The invention aims to solve the technical problem of providing a preparation method of a nano enzyme compound for treating tumors, which can realize the preparation of the nano enzyme compound of FePOs, CNT, GO or FeP, CNT, GO with various enzyme activities and multi-stimulus responses, can realize the combination of various stimulus conditions and enzyme activities with the anti-bone tumor activity, and reveals the molecular biological mechanism of the nano enzyme under the synergistic effect of stimulus and enzyme activities, thereby laying a theoretical foundation for the anti-tumor effect of the stimulus combination nano enzyme.
The technical scheme of the invention is as follows: a method of preparing a nanoenzyme complex for treating a tumor, the nanoenzyme complex being a FePOs, CNT, GO complex or a FeP, CNT, GO complex, comprising the steps of:
step one: putting the CNT and the steel ball into a ball milling tank according to the mass ratio of 45-55:0.5-1.5, adding absolute ethyl alcohol to immerse the steel ball, performing ball milling for 3-5 hours, then taking out the CNT, washing the CNT with absolute ethyl alcohol and deionized water, and drying for later use;
step two: taking 45-55mgCNT after ball milling and drying in the first step, adding 45-55ml deionized water for ultrasonic dispersion for 0.5-1.5h for standby;
step three: taking a beaker, sequentially adding 2-4g of urea, 0.2-0.3g of sodium dodecyl sulfate and 32-36ml of deionized water into the beaker, fully dissolving the solution, then adding 40-60ml of the CNT solution dispersed in the second step, and uniformly stirring the solution;
step four: weighing 0.05-0.15g of ferric sulfate, dissolving in 6-10ml of deionized water, slowly dripping into the solution uniformly stirred in the step three, and continuously stirring for 15-25min;
step five: weighing 0.2-0.3g of phosphoric acid, dissolving in 6-10ml of deionized water, slowly dripping the solution obtained after the uniform stirring in the step four, and continuously stirring for 15-25min;
step six: placing the mixed solution after being uniformly stirred in the step five into a reaction kettle, heating to 135-145 ℃, performing hydrothermal reaction for 1.5-2.5h, cooling to room temperature, performing suction filtration, then washing with deionized water and absolute ethyl alcohol for 2-4 times to obtain a FePOs and CNT composite, drying at 55-65 ℃, and placing into a drying tower for standby;
step seven: placing the FePOs and CNT composite material placed into a drying tower in the step six into a tube furnace, introducing H2, calcining at a high temperature of 840-860 ℃ for 1.5-2.5H to obtain a FeP and CNT composite, taking out the FeP and CNT composite after cooling, and placing the FeP and CNT composite into the drying tower for standby;
step eight: weighing 40-60mg of FeP and CNT composite in the drying tower in the step seven, and grinding the composite with 90-110mg of ionic surfactant for later use;
step nine: weighing 90-110mg of ionic surfactant, dissolving in deionized water, stirring uniformly, slowly adding the ground sample in the step eight, performing ultrasonic treatment for 0.5-1.5h, standing for 25-35min, removing large sediment at the bottom, and taking upper black dispersion liquid for later use;
step ten: dissolving 8-12ml of prepared GO liquid in 380-400ml of deionized water, and performing ultrasonic dispersion for 25-35min;
step eleven: slowly conveying the upper layer black dispersion liquid CNT dispersion liquid prepared in the step nine into the GO liquid prepared in the step ten through a constant flow pump, and carrying out electrostatic self-assembly for 1.5-2.5h;
step twelve: weighing 450-550mg of ammonium bicarbonate, dissolving in 8-12ml of deionized water, slowly adding into the mixed solution obtained after the step eleven electrostatic self-assembly, performing electrostatic self-assembly again for 25-35min, and then performing suction filtration on the obtained solution to obtain a compound of black precipitate FeP, CNT, GO, washing 2-4 times with deionized water and absolute ethyl alcohol, drying and placing into a drying tower for standby.
Preferred as the present invention: the weight percentage of the phosphoric acid is 18-22%.
Preferred as the present invention: the ionic surfactant is 3-sulfopropyl tetradecyl dimethyl betaine.
The beneficial effects of the invention are as follows:
1. the enzyme activity of the nano enzyme can be enhanced under the mild stimulation condition, and the synergistic effect of the nano enzyme and the stimulation condition can achieve a remarkable anti-tumor effect;
2. FePOs, CNT, GO or FeP, CNT, GO nanoenzyme complexes possessing multiple enzymatic activities and capable of multiple stimulus responses can be prepared;
3. the enzyme activity is enhanced under the stimulation response, the tumor cells are killed, and the strategy of combining the stimulation response with the enzyme catalytic reaction provides a new idea for tumor treatment.
Description of the drawings:
FIG. 1 is a graph showing the magnetic properties of FePOs, CNT, GO or FeP, CNT, GO nanoenzyme complexes prepared by the method of the present invention;
FIG. 2 is a schematic diagram showing magnetic properties of FePOs, CNT, GO nano-enzyme complex or FeP, CNT, GO nano-enzyme complex prepared by the preparation method according to the embodiment of the present invention;
FIG. 3 is a graph showing the photo-thermal properties of FePOs, CNT, GO or FeP, CNT, GO nanoenzyme complexes prepared by the method of the present invention;
FIG. 4 is a graph showing peroxidase activity of FePOs, CNT, GO nano-enzyme complex or FeP, CNT, GO nano-enzyme complex prepared by the method of the present invention;
FIG. 5 is a chart showing the anti-tumor performance of FePOs, CNT, GO nano-enzyme complex or FeP, CNT, GO nano-enzyme complex prepared by the preparation method according to the embodiment of the present invention under different stimuli.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Example 1:
a method of preparing a nanoenzyme complex for treating a tumor, the nanoenzyme complex being a FePOs, CNT, GO complex or a FeP, CNT, GO complex, comprising the steps of:
step one: putting the CNT and the steel ball into a ball milling tank according to the mass ratio of 45:0.5, adding absolute ethyl alcohol to immerse the steel ball, performing ball milling for 3 hours, then taking out the CNT, washing the CNT with absolute ethyl alcohol and deionized water, and drying for later use;
step two: taking 45mgCNT after ball milling and drying in the first step, adding 45ml deionized water for ultrasonic dispersion for 0.5h for later use;
step three: taking a beaker, sequentially adding 2g of urea, 0.2g of sodium dodecyl sulfate and 32ml of deionized water into the beaker, fully dissolving the mixture, then adding the 40CNT solution dispersed in the step two, and uniformly stirring the mixture;
step four: weighing 0.05g of ferric sulfate, dissolving in 6ml of deionized water, slowly dripping into the solution uniformly stirred in the step three, and continuously stirring for 15min;
step five: weighing 0.2g of phosphoric acid, dissolving in 6ml of deionized water, slowly dropwise adding the solution obtained after uniform stirring in the step four, and continuously stirring for 15min;
step six: putting the mixed solution after being uniformly stirred in the step five into a reaction kettle, heating to 135 ℃, carrying out hydrothermal reaction for 1.5 hours, cooling to room temperature, carrying out suction filtration, then washing with deionized water and absolute ethyl alcohol for 2 times to obtain a FePOs and CNT composite, drying at 55 ℃, and putting into a drying tower for standby;
step seven: placing the FePOs and CNT composite material placed into a drying tower in the step six into a tube furnace, introducing H2, calcining at a high temperature of 840 ℃ for 1.5 hours to obtain a FeP and CNT composite, taking out the FeP and CNT composite after cooling, and placing the FeP and CNT composite material into the drying tower for standby;
step eight: weighing 40mg of FeP and CNT composite in the drying tower in the step seven, and grinding the composite with 90mg of ionic surfactant for later use;
step nine: weighing 90mg of ionic surfactant, dissolving in deionized water, stirring uniformly, slowly adding the ground sample in the step eight, performing ultrasonic treatment for 0.5h, standing for 25min, removing large sediment at the bottom, and taking upper black dispersion liquid for later use;
step ten: dissolving 8ml of prepared GO liquid in 380ml of deionized water, and performing ultrasonic dispersion for 25min;
step eleven: slowly conveying the upper layer black dispersion liquid CNT dispersion liquid prepared in the step nine into the GO liquid prepared in the step ten through a constant flow pump, and carrying out electrostatic self-assembly for 1.5h;
step twelve: weighing 450mg of ammonium bicarbonate, dissolving in 8ml of deionized water, slowly adding into the mixed solution obtained after the step eleven electrostatic self-assembly, performing electrostatic self-assembly again for 25min, and performing suction filtration on the obtained solution to obtain a compound of black precipitate FeP, CNT, GO, washing with deionized water and absolute ethyl alcohol for 2 times, drying, and placing into a drying tower for standby.
In the above examples, the weight percentage of phosphoric acid was 18%.
In the above examples, the ionic surfactant was 3-sulfopropyl tetradecyl dimethyl betaine.
Example 2:
a method of preparing a nanoenzyme complex for treating a tumor, the nanoenzyme complex being a FePOs, CNT, GO complex or a FeP, CNT, GO complex, comprising the steps of:
step one: putting the CNT and the steel ball into a ball milling tank according to the mass ratio of 50:1, adding absolute ethyl alcohol to immerse the steel ball, performing ball milling for 4 hours, then taking out the CNT, cleaning the CNT with absolute ethyl alcohol and deionized water, and drying for later use;
step two: taking 50mgCNT after ball milling and drying in the first step, adding 50ml deionized water for ultrasonic dispersion for 1h for later use;
step three: taking a beaker, sequentially adding 3g of urea, 0.25g of sodium dodecyl sulfate and 34ml of deionized water into the beaker, fully dissolving the mixture, then adding 50ml of the CNT solution dispersed in the second step, and uniformly stirring the mixture;
step four: weighing 0.1g of ferric sulfate, dissolving in 8ml of deionized water, slowly dripping into the solution uniformly stirred in the step three, and continuously stirring for 20min;
step five: weighing 0.25g of phosphoric acid, dissolving in 8ml of deionized water, slowly dropwise adding the solution obtained after uniform stirring in the step four, and continuously stirring for 20min;
step six: putting the mixed solution after being uniformly stirred in the step five into a reaction kettle, heating to 140 ℃, carrying out hydrothermal reaction for 2 hours, cooling to room temperature, carrying out suction filtration, then washing 3 times with deionized water and absolute ethyl alcohol to obtain a FePOs and CNT composite, drying at 60 ℃, and putting into a drying tower for standby;
step seven: placing the FePOs and CNT composite material placed into a drying tower in the step six into a tube furnace, introducing H2, calcining at a high temperature of 850 ℃ for 2 hours to obtain a FeP and CNT composite, taking out the FeP and CNT composite after cooling, and placing the FeP and CNT composite into the drying tower for standby;
step eight: weighing 50mg of FeP and CNT composite in the drying tower in the step seven, and grinding the composite with 100mg of ionic surfactant for later use;
step nine: weighing 100mg of ionic surfactant, dissolving in deionized water, stirring uniformly, slowly adding the ground sample in the step eight, performing ultrasonic treatment for 1h, standing for 30min, removing large sediment at the bottom, and taking upper black dispersion liquid for later use;
step ten: dissolving 10ml of prepared GO liquid in 390ml of deionized water, and performing ultrasonic dispersion for 30min;
step eleven: slowly conveying the upper layer black dispersion liquid CNT dispersion liquid prepared in the step nine into the GO liquid prepared in the step ten through a constant flow pump, and carrying out electrostatic self-assembly for 2h;
step twelve: weighing 500mg of ammonium bicarbonate, dissolving in 10ml of deionized water, slowly adding into the mixed solution obtained after the step eleven electrostatic self-assembly, performing electrostatic self-assembly again for 30min, and performing suction filtration on the obtained solution to obtain a compound of black precipitate FeP, CNT, GO, washing 3 times with deionized water and absolute ethyl alcohol, drying and placing into a drying tower for standby.
In the above examples, the weight percentage of phosphoric acid was 20%.
In the above examples, the ionic surfactant was 3-sulfopropyl tetradecyl dimethyl betaine.
Example 3:
a method of preparing a nanoenzyme complex for treating a tumor, the nanoenzyme complex being a FePOs, CNT, GO complex or a FeP, CNT, GO complex, comprising the steps of:
step one: putting the CNT and the steel ball into a ball milling tank according to the mass ratio of 55:1.5, adding absolute ethyl alcohol to immerse the steel ball, performing ball milling for 5 hours, then taking out the CNT, washing the CNT with absolute ethyl alcohol and deionized water, and drying for later use;
step two: taking 55mgCNT after ball milling and drying in the first step, adding 55ml deionized water for ultrasonic dispersion for 1.5 hours for later use;
step three: taking a beaker, sequentially adding 4g of urea, 0.3g of sodium dodecyl sulfate and 36ml of deionized water into the beaker, fully dissolving the mixture, then adding 60ml of the CNT solution dispersed in the second step, and uniformly stirring the mixture;
step four: weighing 0.15g of ferric sulfate, dissolving in 10ml of deionized water, slowly dripping into the solution uniformly stirred in the step three, and continuously stirring for 25min;
step five: weighing 0.3g of phosphoric acid, dissolving in 10ml of deionized water, slowly dropwise adding the solution obtained after uniform stirring in the step four, and continuously stirring for 25min;
step six: putting the mixed solution after being uniformly stirred in the step five into a reaction kettle, heating to 145 ℃, carrying out hydrothermal reaction for 2.5 hours, cooling to room temperature, carrying out suction filtration, then washing with deionized water and absolute ethyl alcohol for 4 times to obtain a FePOs and CNT composite, drying at 65 ℃, and putting into a drying tower for standby;
step seven: placing the FePOs and CNT composite material placed into a drying tower in the step six into a tube furnace, introducing H2, calcining at high temperature for 2.5H at 860 ℃ to obtain a FeP and CNT composite, taking out the FeP and CNT composite after cooling, and placing the FeP and CNT composite into the drying tower for standby;
step eight: weighing 60mg of FeP and CNT composite in the drying tower in the step seven, and grinding the composite with 110mg of ionic surfactant for later use;
step nine: weighing 110mg of ionic surfactant, dissolving in deionized water, stirring uniformly, slowly adding the ground sample in the step eight, performing ultrasonic treatment for 1.5h, standing for 35min, removing large sediment at the bottom, and taking upper black dispersion liquid for later use;
step ten: dissolving 12ml of prepared GO liquid in 400ml of deionized water, and performing ultrasonic dispersion for 35min;
step eleven: slowly conveying the upper layer black dispersion liquid CNT dispersion liquid prepared in the step nine into the GO liquid prepared in the step ten through a constant flow pump, and carrying out electrostatic self-assembly for 2.5h;
step twelve: weighing 550mg of ammonium bicarbonate, dissolving in 12ml of deionized water, slowly adding into the mixed solution obtained after the step eleven electrostatic self-assembly, performing electrostatic self-assembly again for 35min, and performing suction filtration on the obtained solution to obtain a compound of black precipitate FeP, CNT, GO, washing with deionized water and absolute ethyl alcohol for 4 times, drying, and placing into a drying tower for standby.
In the above examples, the weight percentage of phosphoric acid was 22%.
In the above examples, the ionic surfactant was 3-sulfopropyl tetradecyl dimethyl betaine.
As shown in fig. 1 and 2, the magnetic properties of FePOs, CNT, GO nano-enzyme complex or FeP, CNT, GO nano-enzyme complex prepared by the preparation method according to the embodiment of the present invention are shown.
As shown in the following table, the following table shows the conductivities of FePOs, CNT, GO nano-enzyme complexes or FeP, CNT, GO nano-enzyme complexes prepared by the preparation methods described in the examples of the present invention;
as shown in fig. 3, fig. 3 is a photo-thermal diagram of FePOs, CNT, GO nano-enzyme complex or FeP, CNT, GO nano-enzyme complex prepared by the preparation method according to the embodiment of the present invention.
As shown in fig. 4, fig. 4 is a graph showing peroxidase activity of FePOs, CNT, GO nano-enzyme complex or FeP, CNT, GO nano-enzyme complex prepared by the preparation method according to the example of the present invention.
As shown in fig. 5, fig. 5 is a graph showing the antitumor performance of FePOs, CNT, GO nano-enzyme complex or FeP, CNT, GO nano-enzyme complex prepared by the preparation method according to the embodiment of the present invention under different stimuli.
According to the preparation method of the nano enzyme complex for treating tumors, which is disclosed in the embodiment, fePOs, CNT, GO nano enzyme complex or FeP, CNT, GO nano enzyme complex with various enzyme activities and multiple stimulus responses can be prepared, and the nano enzyme complex has peroxidase activity, catalase activity and superoxide dismutase activity, can respond to magnetic, electric and optical stimulus, can realize that the enzyme activity of the nano enzyme is enhanced under mild stimulus conditions, and can achieve remarkable anti-tumor effect through synergistic effect with the stimulus conditions, and the research on the anti-tumor cytomolecular mechanism of the nano enzyme is carried out;
the prepared FePOs, CNT, GO nano enzyme complex or FeP, CNT, GO nano enzyme complex can realize various stimulus responses, the enzyme activity is enhanced under the stimulus responses, the tumor cells are killed, and a new thought is provided for tumor treatment by combining the stimulus responses with a strategy of enzyme catalytic reaction;
FePOs, CNT, GO nanometer enzyme complex or FeP,The CNT and GO nano enzyme complex can play an anti-tumor activity effect under the condition of no drug effect, and under the condition of applying exogenous stimulus, the exogenous stimulus (such as electricity, magnetism, light, heat and the like) can cooperate with nano enzyme to resist tumor, and the endogenous high H of the tumor 2 O 2 By peroxidase activity in combination with exogenous stimulation, and by SOD enzyme activity and catalase activity, to perform the next stage of sustained action after one stage of tumor treatment.
The foregoing examples merely illustrate specific embodiments of the invention, which are described in greater detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention.
Claims (3)
1. A method for preparing a nano-enzyme complex for treating tumors, which is a FeP, CNT, GO complex, comprising the steps of:
step one: putting the CNT and the steel ball into a ball milling tank according to the mass ratio of 45-55:0.5-1.5, adding absolute ethyl alcohol to immerse the steel ball, performing ball milling for 3-5 hours, then taking out the CNT, washing the CNT with absolute ethyl alcohol and deionized water, and drying for later use;
step two: taking 45-55mgCNT after ball milling and drying in the first step, adding 45-55ml deionized water for ultrasonic dispersion for 0.5-1.5h for standby;
step three: taking a beaker, sequentially adding 2-4g of urea, 0.2-0.3g of sodium dodecyl sulfate and 32-36ml of deionized water into the beaker, fully dissolving the solution, then adding 40-60ml of the CNT solution dispersed in the second step, and uniformly stirring the solution;
step four: weighing 0.05-0.15g of ferric sulfate, dissolving in 6-10ml of deionized water, slowly dripping into the solution uniformly stirred in the step three, and continuously stirring for 15-25min;
step five: weighing 0.2-0.3g of phosphoric acid, dissolving in 6-10ml of deionized water, slowly dripping the solution obtained after the uniform stirring in the step four, and continuously stirring for 15-25min;
step six: placing the mixed solution after being uniformly stirred in the step five into a reaction kettle, heating to 135-145 ℃, performing hydrothermal reaction for 1.5-2.5h, cooling to room temperature, performing suction filtration, then washing with deionized water and absolute ethyl alcohol for 2-4 times to obtain a FePOs and CNT composite, drying at 55-65 ℃, and placing into a drying tower for standby;
step seven: putting the FePOs and CNT composite material put into the drying tower in the step six into a tube furnace, and introducing H 2 Calcining at 840-860 ℃ for 1.5-2.5h to obtain FeP and CNT composite, cooling, taking out the FeP and CNT composite, and placing into a drying tower for standby;
step eight: weighing 40-60mg of FeP and CNT composite in the drying tower in the step seven, and grinding the composite with 90-110mg of ionic surfactant for later use;
step nine: weighing 90-110mg of ionic surfactant, dissolving in deionized water, stirring uniformly, slowly adding the ground sample in the step eight, performing ultrasonic treatment for 0.5-1.5h, standing for 25-35min, removing large sediment at the bottom, and taking upper black dispersion liquid for later use;
step ten: dissolving 8-12ml of prepared GO liquid in 380-400ml of deionized water, and performing ultrasonic dispersion for 25-35min;
step eleven: slowly conveying the upper layer black dispersion liquid prepared in the step nine, namely FeP and CNT composite dispersion liquid, into the GO liquid prepared in the step ten through a constant flow pump, and carrying out electrostatic self-assembly for 1.5-2.5h;
step twelve: weighing 450-550mg of ammonium bicarbonate, dissolving in 8-12ml of deionized water, slowly adding into the mixed solution obtained after the step eleven electrostatic self-assembly, performing electrostatic self-assembly again for 25-35min, and then performing suction filtration on the obtained solution to obtain a compound of black precipitate FeP, CNT, GO, washing 2-4 times with deionized water and absolute ethyl alcohol, drying and placing into a drying tower for standby.
2. The method for preparing a nano-enzyme complex for treating tumors according to claim 1, wherein the weight percentage of the phosphoric acid is 18-22%.
3. The method for preparing a nano-enzyme complex for treating tumors according to claim 1 or 2, wherein the ionic surfactant is 3-sulfopropyl tetradecyl dimethyl betaine.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107552083A (en) * | 2017-10-11 | 2018-01-09 | 肇庆市华师大光电产业研究院 | A kind of FeP/C3N4Composite photo-catalyst and its preparation method and application |
| WO2019134741A1 (en) * | 2018-01-03 | 2019-07-11 | Albert-Ludwigs-Universität Freiburg | Single-channel multianalyte biosensor |
| CN111467563A (en) * | 2020-03-06 | 2020-07-31 | 西南交通大学 | RGO/CNT/HA/Fe3O4Synthesis method of composite material |
| CN111715249A (en) * | 2020-07-22 | 2020-09-29 | 陕西科技大学 | Spherical FeP electrocatalyst and preparation method thereof |
| CN112604717A (en) * | 2020-12-29 | 2021-04-06 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of quantum dot iron-based metal organic framework composite material |
| CN113289650A (en) * | 2021-06-08 | 2021-08-24 | 华东师范大学 | Bimetallic phosphide-carbon electrocatalytic hydrogen evolution material and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040202603A1 (en) * | 1994-12-08 | 2004-10-14 | Hyperion Catalysis International, Inc. | Functionalized nanotubes |
-
2022
- 2022-06-13 CN CN202210662888.XA patent/CN115137820B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107552083A (en) * | 2017-10-11 | 2018-01-09 | 肇庆市华师大光电产业研究院 | A kind of FeP/C3N4Composite photo-catalyst and its preparation method and application |
| WO2019134741A1 (en) * | 2018-01-03 | 2019-07-11 | Albert-Ludwigs-Universität Freiburg | Single-channel multianalyte biosensor |
| CN111467563A (en) * | 2020-03-06 | 2020-07-31 | 西南交通大学 | RGO/CNT/HA/Fe3O4Synthesis method of composite material |
| CN111715249A (en) * | 2020-07-22 | 2020-09-29 | 陕西科技大学 | Spherical FeP electrocatalyst and preparation method thereof |
| CN112604717A (en) * | 2020-12-29 | 2021-04-06 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of quantum dot iron-based metal organic framework composite material |
| CN113289650A (en) * | 2021-06-08 | 2021-08-24 | 华东师范大学 | Bimetallic phosphide-carbon electrocatalytic hydrogen evolution material and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| FeP改性的Pt/FeP/CNTs催化剂的制备和电化学性能研究;李翔;李加琪;罗兰萍;梁鸿皓;;广东石油化工学院学报(第01期);全文 * |
| Nitrogen-doped carbon nanotube supported iron phosphide nanocomposites for highly active electrocatalysis of the hydrogen evolution reaction;Qian Liu等;Electrochimica Acta;第149卷;全文 * |
| 磷化铁/石墨烯纳米复合物的制备及其在F-T合成反应中的应用;杨敬贺;赵博;赵华博;陆安慧;马丁;;化学学报(第10期);全文 * |
| 纳米酶:疾病治疗新选择;侯亚欣;张若飞;阎锡蕴;范克龙;;中国科学:生命科学(第03期);全文 * |
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