CN115125164A - Highly salt-tolerant and heavy metal-resistant heterotrophic nitrification-aerobic denitrification self-flocculation marine bacterium and application thereof in community construction - Google Patents
Highly salt-tolerant and heavy metal-resistant heterotrophic nitrification-aerobic denitrification self-flocculation marine bacterium and application thereof in community construction Download PDFInfo
- Publication number
- CN115125164A CN115125164A CN202210562929.8A CN202210562929A CN115125164A CN 115125164 A CN115125164 A CN 115125164A CN 202210562929 A CN202210562929 A CN 202210562929A CN 115125164 A CN115125164 A CN 115125164A
- Authority
- CN
- China
- Prior art keywords
- wastewater
- heterotrophic nitrification
- aerobic denitrification
- salinity
- salt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 22
- 238000010276 construction Methods 0.000 title claims abstract description 8
- 238000005189 flocculation Methods 0.000 title abstract description 10
- 241000556533 uncultured marine bacterium Species 0.000 title abstract description 6
- 239000002351 wastewater Substances 0.000 claims abstract description 53
- 241000589516 Pseudomonas Species 0.000 claims abstract description 34
- 244000005700 microbiome Species 0.000 claims abstract description 32
- 239000002689 soil Substances 0.000 claims abstract description 15
- 229910052793 cadmium Inorganic materials 0.000 claims abstract description 10
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000004062 sedimentation Methods 0.000 claims abstract description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 48
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 241000894006 Bacteria Species 0.000 claims description 35
- 238000000034 method Methods 0.000 claims description 30
- 229910052757 nitrogen Inorganic materials 0.000 claims description 24
- 239000013535 sea water Substances 0.000 claims description 22
- 241000122229 Acinetobacter johnsonii Species 0.000 claims description 20
- 230000000813 microbial effect Effects 0.000 claims description 16
- 239000010802 sludge Substances 0.000 claims description 13
- 241000589291 Acinetobacter Species 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 9
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 230000006872 improvement Effects 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 7
- 238000005273 aeration Methods 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 238000012163 sequencing technique Methods 0.000 claims description 6
- 239000003513 alkali Substances 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 4
- 238000012851 eutrophication Methods 0.000 claims description 4
- 230000014759 maintenance of location Effects 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 239000003895 organic fertilizer Substances 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 2
- 235000019270 ammonium chloride Nutrition 0.000 claims description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 2
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 2
- 230000035558 fertility Effects 0.000 claims description 2
- 239000013505 freshwater Substances 0.000 claims description 2
- 238000009364 mariculture Methods 0.000 claims description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 239000001632 sodium acetate Substances 0.000 claims description 2
- 235000017281 sodium acetate Nutrition 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims 4
- 230000015784 hyperosmotic salinity response Effects 0.000 abstract description 2
- 241001288557 Pseudomonas kunmingensis Species 0.000 description 25
- 229910002651 NO3 Inorganic materials 0.000 description 21
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 20
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 18
- 239000001963 growth medium Substances 0.000 description 16
- 230000001580 bacterial effect Effects 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 230000008569 process Effects 0.000 description 10
- 239000002609 medium Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 239000006228 supernatant Substances 0.000 description 8
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 7
- 230000012010 growth Effects 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 241001052560 Thallis Species 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000002068 microbial inoculum Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000002798 spectrophotometry method Methods 0.000 description 4
- 235000013619 trace mineral Nutrition 0.000 description 4
- 239000011573 trace mineral Substances 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 3
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000001546 nitrifying effect Effects 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- DIOQZVSQGTUSAI-UHFFFAOYSA-N decane Chemical compound CCCCCCCCCC DIOQZVSQGTUSAI-UHFFFAOYSA-N 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- SNRUBQQJIBEYMU-UHFFFAOYSA-N dodecane Chemical compound CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- ZPLCXHWYPWVJDL-UHFFFAOYSA-N 4-[(4-hydroxyphenyl)methyl]-1,3-oxazolidin-2-one Chemical compound C1=CC(O)=CC=C1CC1NC(=O)OC1 ZPLCXHWYPWVJDL-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 241001012632 Pseudomonas sp. HSI-7 Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000004099 anaerobic respiration Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 239000007640 basal medium Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 125000001475 halogen functional group Chemical group 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910052901 montmorillonite Inorganic materials 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 239000010695 polyglycol Substances 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 239000004562 water dispersible granule Substances 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Biochemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Soil Sciences (AREA)
- Pest Control & Pesticides (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention provides a heterotrophic nitrification-aerobic denitrification self-flocculation marine bacterium with high salt tolerance and heavy metal tolerance and application thereof in community construction, belonging to the technical field of microorganism and environment treatment and restoration. The screened strain of pseudomonas Kunming (Pseudomonas kunming) 8-C which can efficiently denitrify by using a heterotrophic nitrification-aerobic denitrification way and has self-flocculation capability has wide application value in treating high-salinity wastewater and lead and cadmium polluted wastewater and repairing soil and also has the potential of developing a heterotrophic nitrification-aerobic denitrification community with good sedimentation performance. Therefore, it has good practical application value.
Description
Technical Field
The invention belongs to the technical field of microorganism and environment treatment and restoration, and particularly relates to a highly salt-tolerant heavy metal-resistant heterotrophic nitrification-aerobic denitrification self-flocculation marine bacterium and application thereof in community construction.
Background
The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.
Over standard of nitrogen-containing compounds in the water body can cause eutrophication of the water body, algae and other plankton are caused to rapidly propagate, the dissolved oxygen content of the water body is reduced, the water quality is deteriorated, fishes and other organisms die in large quantity, and the ecological balance and the human health are seriously harmed. The biological denitrification method is widely applied to the nitrogen pollution treatment of wastewater due to the characteristics of economy, high efficiency, no secondary pollution and the like. The traditional biological denitrification technology needs to go through two steps, namely an aerobic nitrification process of autotrophic nitrifying bacteria and an anaerobic denitrification process of heterotrophic denitrifying bacteria. The anoxic-aerobic method is the most basic engineering example of the traditional biological denitrification technology. In the anoxic section, denitrifying bacteria take organic carbon in sewage as an electron donor and nitrate as an electron acceptor to perform anaerobic respiration, reduce nitrate nitrogen in the reflux liquid into nitrogen and release the nitrogen to complete the denitrification process. In the aerobic section, nitrifying bacteria oxidize ammonia nitrogen in the sewage into nitrate and then flow back to the anoxic tank. This technique requires strict control of aerobic and anaerobic conditions in two stages and therefore must be run in two separate structures. Moreover, the nitrifying bacteria have long generation time and need longer sludge retention time. Bacteria performing both functions have more differences in growth conditions and metabolic characteristics, etc., thereby increasing investment costs and complexity of regulation in practical applications. Therefore, the search for more economical, more convenient and more efficient methods becomes an important issue to be paid attention to for biological denitrification.
In recent years, researchers have found heterotrophic nitrification-aerobic denitrification bacteria from the environment, and the microorganisms can simultaneously carry out nitrification and denitrification under aerobic conditions. The discovery of the process breaks through the bottleneck of biological denitrification technology. However, the inventor finds that most heterotrophic nitrification-aerobic denitrification strains screened at present need to grow in a mild and appropriate environment, have high requirements on environmental conditions, cannot be efficiently denitrified in high-salt heavy metal wastewater, have poor settling performance, are not suitable for being applied in practical engineering, and most researches are limited to single bacteria at present, so that a microorganism which can still efficiently degrade nitrogen pollutants in extreme environments such as saline-alkali environment, heavy metal environment and the like needs to be searched, and the potential of the microorganism in the aspects of synthesizing communities, constructing microorganism groups and the like is developed.
Disclosure of Invention
Based on the defects of the prior art, the invention provides a heterotrophic nitrification-aerobic denitrification self-flocculation marine bacterium with high salt tolerance and heavy metal tolerance and application thereof in community construction. The screened strain of Pseudomonas kunmingensis (Pseudomonas kunmingensis) 8-C which can efficiently denitrify by using a heterotrophic nitrification-aerobic denitrification way and has self-flocculation capability has wide application value in treating high-salinity wastewater and lead and cadmium polluted wastewater and repairing soil and also has the potential of developing a heterotrophic nitrification-aerobic denitrification community with good sedimentation performance. The present invention has been completed based on the above results.
In order to achieve the technical purpose, the invention relates to the following technical scheme:
one aspect of the invention provides a heterotrophic nitrification-aerobic denitrification strain, which is classified and named as Kunming pseudomonads (Pseudomonas kunmingensis) 8-C, and the strain is preserved in China center for type culture Collection (address: Wuchang Lojia mountain Wuhan university, Wuhan, Hubei province) 12 and 13 months in 2021, and the biological preservation number is CCTCC NO: m20211592.
In a second aspect of the invention, a microbial agent is provided, wherein the microbial agent comprises the heterotrophic nitrification-aerobic denitrification bacteria 8-C.
In a third aspect of the present invention, there is provided a halophilic heterotrophic nitrification-aerobic denitrification microbiome comprising the heterotrophic nitrification-aerobic denitrification bacteria 8-C; more specifically, the halophilic heterotrophic nitrification-aerobic denitrification microbiome is obtained by inoculating the heterotrophic nitrification-aerobic denitrification bacteria 8-C and Acinetobacter johnsonii (Acinetobacter johnsonii)2-1-H into high-salinity wastewater for culture; the halophilic heterotrophic nitrification-aerobic denitrification microbiome composed of the heterotrophic nitrification-aerobic denitrification bacteria 8-C and the Acinetobacter johnsonii2-1-H has good and stable treatment effect, the ammonia nitrogen removal rate in the simulated high-salinity wastewater can reach 90 percent, the total nitrogen removal rate can reach 85 percent, and almost no nitrite and nitrate are accumulated.
Wherein the Acinetobacter johnsonii (Acinetobacter johnsonii)2-1-H has been preserved in the China center for type culture Collection at 12 months and 13 days in 2021, (address: Wuhan university of Lojia mountain of Wuchang, Wuhan, Hubei), and the biological preservation number is CCTCC NO: m20211593.
In a fourth aspect of the present invention, there is provided a method for constructing the above halophilic heterotrophic nitrification-aerobic denitrification microbiome, the method comprising:
inoculating the heterotrophic nitrification-aerobic denitrification bacteria 8-C and the Acinetobacter johnsonii2-1-H into a bioreactor containing high-salinity wastewater, and operating until the ammonia nitrogen removal efficiency and the sedimentation performance are stabilized, thereby obtaining the halophilic heterotrophic nitrification-aerobic denitrification microorganism group.
The reactor does not discharge sludge during operation, so that the salt-adapted heterotrophic nitrification-aerobic denitrification microorganism group is more easily obtained.
In a fifth aspect of the present invention, the heterotrophic nitrification-aerobic denitrification bacteria, the microbial inoculum and/or the halophilic heterotrophic nitrification-aerobic denitrification microbiome are used in any one or more of the following applications:
a) treating waste water;
b) preparing an organic fertilizer;
c) soil improvement;
d) treating water eutrophication;
e) repairing water body pollution;
f) biological denitrification.
In a sixth aspect of the present invention, a method for the integrated resource transformation of nutrients in a high-salt environment is provided, which comprises: and (3) applying the heterotrophic nitrification-aerobic denitrification bacteria, the microbial agent and/or the halophilic heterotrophic nitrification-aerobic denitrification microbial group to a high-salt environment.
More specifically, the high-salt environment is a high-salt water environment, and further can be a high-salt water environment polluted by heavy metals; the salinity of the high-salinity water environment is not lower than 3 percent, and the salinity is further preferably 3 to 8 percent; the heavy metals include, but are not limited to, lead and cadmium.
The beneficial technical effects of one or more technical schemes are as follows:
according to the technical scheme, a Kunming Pseudomonas (Pseudomonas kunmingensis) 8-C which can efficiently denitrify by using a heterotrophic nitrification-aerobic denitrification way and has self-flocculation capability is obtained through screening and separation, and researches show that the bacterial strain can utilize ammonia nitrogen and nitrate as nitrogen sources under aerobic conditions, does not accumulate nitrite nitrogen in the ammonia nitrogen conversion process, generates a small amount of nitrate and then removes the nitrate, has wide tolerance range on salinity and pH, and has good tolerance on heavy metals such as lead, cadmium and the like; meanwhile, further research finds that the heterotrophic nitrification-aerobic denitrification microbial group which is synthesized with Acinetobacter johnsonii (Acinetobacter johnsonii)2-1-H and developed by the method has good settling property and stable and efficient denitrification capability, can achieve 90% of ammonia nitrogen removal rate and 85% of total nitrogen removal rate in simulated high-salt wastewater, and hardly causes the accumulation of nitrite and nitrate, so that the method has wide industrial application prospect in biological denitrification pollution treatment, particularly in ammonia nitrogen wastewater treatment of high-salt and heavy metal pollution.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are included to illustrate an exemplary embodiment of the invention and not to limit the invention.
FIG. 1 is a phylogenetic tree of the halophilic heterotrophic nitrification-aerobic denitrification bacterium Pseudomonas Kunmingensis (Pseudomonas kunmingensis) 8-C of the present invention;
FIG. 2 is a macroscopic and microscopic surface morphology of Pseudomonas kunmingensis (Pseudomonas kunmingensis) 8-C of the present invention;
FIG. 3 shows the effect of Pseudomonas kunmingensis (Pseudomonas kunmingensis) 8-C in removing ammonia nitrogen and nitrate nitrogen;
FIG. 4 shows the total nitrogen balance of Pseudomonas kunmingensis (Pseudomonas kunmingensis) 8-C in the present invention when ammonia nitrogen and nitrate are used;
FIG. 5 shows the effect of Pseudomonas kunmingensis (Pseudomonas kunmingensis) 8-C in removing ammonia nitrogen under different salinity conditions;
FIG. 6 shows the effect of Pseudomonas kunmingensis (Pseudomonas kunmingensis) 8-C in removing ammonia nitrogen under different pH conditions;
FIG. 7 shows a reaction product of Pseudomonas kunmingensis (Pseudomonas kunmingensis 8-C) in the presence of Pb 2+ 、Cd 2+ The effect of removing ammonia nitrogen in the simulated wastewater is improved.
FIG. 8 shows the utilization of two halophilic HNAD bacteria in the present invention: pseudomonas kunmingensis (Pseudomonas kunmingensis) 8-C and Acinetobacter johnsonii (Acinetobacter johnsonii) 2-1-H.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
As described above, most heterotrophic nitrification-aerobic denitrification strains screened at present need to grow in a mild and appropriate environment, have high requirements on environmental conditions, cannot efficiently denitrify high-salinity heavy metal wastewater, have poor settling properties, and are not suitable for application in practical engineering.
In view of the above, in one embodiment of the present invention, a heterotrophic nitrification-aerobic denitrification bacterium is provided, which is classified and named as Pseudomonas kunmingensis (Pseudomonas kunmingensis) 8-C, and which has been preserved in the center for type culture collection of china (address: university of armonka mountain wuhan, wuchang, north of lake) at 12-13 months in 2021, and has a biological preservation number of CCTCC NO: m20211592.
The characteristics of the thallus and the bacterial colony are as follows: the bacterial strain is gram-negative bacteria, the bacteria are rod-shaped under the observation of a scanning electron microscope, the size of the bacteria is about 0.5-1.0 mu m, and bacterial colonies on an LB solid culture medium are milky white, moist, smooth and flat.
According to the invention, researches show that pseudomonas kunmingensis 8-C can utilize ammonia nitrogen and nitrate as nitrogen sources under aerobic conditions, no nitrite nitrogen is accumulated in the ammonia nitrogen conversion process, and a small amount of nitrate is generated and then removed. The total nitrogen in the system is converted from a water phase to a gas phase and converted into biomass in the process of utilizing ammonia nitrogen and nitrate by the strain, and about 50 percent of nitrogen is lost in a gas state.
Meanwhile, the strain has higher tolerance to salinity conditions. Compared with low salinity, the strain has higher ammonia nitrogen removal effect under medium and high salinity. In the salinity range of 3-8%, the ammonia nitrogen removal rate exceeds 85%; the ammonia nitrogen removal rate is over 40 percent when the salinity is 1-2 percent. The bacterial strain has wide tolerance range on pH, is more favorable for growth and metabolism under neutral and alkalescent conditions, and has ammonia nitrogen removal efficiency over 90 percent within the pH range of 7-9.
In addition, the strain is resistant to Pb 2+ 、Cd 2+ The heavy metal ions have good tolerance, namely 20mg/L of Pb 2+ The ammonia nitrogen removal rate can reach 85 percent under stress, and the concentration is 10mg/L Cd 2+ The ammonia nitrogen removal rate can reach 80 percent under stress. Thereby further widening the application field and the application range thereof.
In another embodiment of the present invention, there is provided a microbial agent comprising the heterotrophic nitrification-aerobic denitrification bacterium 8-C.
In another embodiment of the present invention, the microbial agent further contains a carrier in addition to the heterotrophic nitrification-aerobic denitrification bacterium 8-C as an active ingredient. The carrier may be one that is commonly used in the field of microbial agents and is biologically inert.
The carrier can be a solid carrier or a liquid carrier;
the solid carrier can be a mineral material, a plant material or a high molecular compound; the mineral material may be at least one of clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica, and diatomaceous earth; the plant material may be at least one of corn flour, bean flour and starch; the high molecular compound can be polyvinyl alcohol or/and polyglycol;
the liquid carrier can be an organic solvent, vegetable oil, mineral oil, or water; the organic solvent may be decane or/and dodecane.
The preparation formulation of the microbial inoculum can be various preparation formulations, such as liquid, emulsion, suspending agent, powder, granules, wettable powder or water dispersible granules.
According to the requirement, the microbial inoculum can also be added with a surfactant (such as Tween 20, Tween 80 and the like), a binder, a stabilizer (such as an antioxidant), a pH regulator and the like.
In another embodiment of the present invention, there is provided a salt-tolerant heterotrophic nitrification-aerobic denitrification microorganism group comprising the heterotrophic nitrification-aerobic denitrification bacteria 8-C; more specifically, the halophilic heterotrophic nitrification-aerobic denitrification microbiome is obtained by inoculating the heterotrophic nitrification-aerobic denitrification bacteria 8-C and Acinetobacter johnsonii (Acinetobacter johnsonii)2-1-H into high-salt wastewater for culture; the halophilic heterotrophic nitrification-aerobic denitrification microbiome composed of the heterotrophic nitrification-aerobic denitrification bacteria 8-C and the Acinetobacter johnsonii2-1-H has good and stable treatment effect, the ammonia nitrogen removal rate in the simulated high-salinity wastewater can reach 90 percent, the total nitrogen removal rate can reach 85 percent, and almost no nitrite and nitrate are accumulated.
Wherein the Acinetobacter johnsonii (Acinetobacter johnsonii)2-1-H is preserved in China center for type culture Collection in 12 months and 13 days in 2021, (address: Wuhan university, Lojia mountain Lojia, Wuchang, Wuhan, Hubei), the biological preservation number is CCTCC NO: m20211593.
In another embodiment of the present invention, the halophilic nitrogen assimilating microorganism group may be high-salinity wastewater obtained after the above treatment or activated sludge obtained after the above treatment.
In another embodiment of the present invention, there is provided a method for constructing the aforementioned halophilic nitrogen assimilating microorganism group, the method comprising:
inoculating the heterotrophic nitrification-aerobic denitrification bacteria 8-C and the Acinetobacter johnsonii2-1-H into a bioreactor containing high-salinity wastewater, and operating until the ammonia nitrogen removal efficiency and the sedimentation performance are stabilized, thereby obtaining the halophilic heterotrophic nitrification-aerobic denitrification microorganism group.
The reactor does not discharge sludge during operation, so that the salt-adapted heterotrophic nitrification-aerobic denitrification microorganism group is more easily obtained.
The high-salinity wastewater can be actual high-salinity wastewater or simulated high-salinity wastewater; the simulated high salinity wastewater can be seawater simulated wastewater, and comprises the following specific components: 3% of seawater element, 500mg/L of sodium acetate, 140mg/L of dipotassium phosphate and 550mg/L of ammonium chloride.
The bioreactor is a Sequencing Batch Reactor (SBR), the sequencing batch reactor is an intermittent activated sludge system adopting a pool body, and the pool body is used as the bioreactor and a sedimentation tank. When treating continuous flow sewage, at least two or more tanks are needed, and an air diffusion device is installed at the bottom of the tank body to play roles of aeration and stirring.
The construction method comprises the following steps:
the continuous operation is adopted, each cycle comprises 5min water inlet, 450min aeration, 15min sedimentation and 10min water outlet, each period is 8h, the volume exchange rate is 62.5%, and the Hydraulic Retention Time (HRT) is 12.8 h.
In another embodiment of the present invention, the heterotrophic nitrification-aerobic denitrification bacteria, the microbial inoculum and/or the halophilic heterotrophic nitrification-aerobic denitrification microbiome are used in any one or more of the following applications:
a) treating wastewater;
b) preparing an organic fertilizer;
c) soil improvement;
d) treating water eutrophication;
e) repairing water body pollution;
f) and (4) biological denitrification.
In the a), the wastewater comprises but is not limited to high-salinity wastewater, mariculture wastewater, industrial saline wastewater and seawater toilet flushing wastewater; the wastewater may also be heavy metal contaminated wastewater, including but not limited to lead and cadmium.
In the c), the soil may be saline-alkali soil; the soil may also be a saline-alkali earth contaminated with heavy metals including, but not limited to, lead and cadmium; the soil improvement may be soil fertility improvement.
In d), the body of water comprises fresh water and seawater, preferably seawater.
In another embodiment of the present invention, a method for the integrated resource transformation of nutrients in a high salinity environment is provided, which comprises: and (3) applying the heterotrophic nitrification-aerobic denitrification bacteria, the microbial agent and/or the halophilic heterotrophic nitrification-aerobic denitrification microbial group to a high-salt environment.
More specifically, the high-salt environment is a high-salt water environment, and further can be a high-salt water environment polluted by heavy metals; the salinity of the high-salinity water environment is not lower than 3 percent, and the salinity is more preferably 3 to 8 percent; including but not limited to lead and cadmium.
The invention is further illustrated by the following examples, which are not to be construed as limiting the invention thereto. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Examples NH 4 + -N、NO 2 - -N、NO 3 - The determination and analysis of-N, TN are both referred to the national Standard, NH 4 + The determination and analysis of-N are carried out according to water quality-determination of ammonia nitrogen-Nessler reagent spectrophotometry (GB HJ 535-2009); NO 2 - Determination and analysis of N according to Water quality-determination of nitrite Nitrogen-spectrophotometry (GB 7493-87); NO (nitric oxide) 3 - The determination and analysis of-N is based on the determination of water quality-nitrate nitrogen-ultraviolet spectrophotometry (GB HJ/T346-); TN was measured and analyzed according to "Water quality-Total Nitrogen determination-ultraviolet Spectrophotometry" (GB 11894-89).
The basal medium used in the examples was autoclaved at 121 ℃ for 20min, and the formulation was as follows:
(1) solution of trace elements (L) -1 )：EDTA50g、ZnSO 4 ·7H 2 O 5.02g、CuSO 4 ·5H 2 O 1.57g、FeSO 4 ·7H 2 O 5.0g、CaCl 2 ·2H 2 O 5.5g、MnCl 2 ·4H 2 O 5.06g、pH 6.0。
(2) Enrichment Medium (L) -1 )：MgSO 4 ·7H 2 O 0.01g、KH 2 PO 4 0.056g、Na 2 HPO 4 7.9g、NH 4 Cl 0.192g、NaNO 3 0.3415g、NaNO 2 0.362g、CH 3 COONa 0.65g, sea water extract 30g and trace element solution 2 mL.
(3) Heterotrophic Nitrification (HNM) prescreening medium (L) -1 )：CH 3 COONa 0.65g、NH 4 Cl 0.192g、KH 2 PO 4 0.056g of seawater extract30g and 2mL of trace element solution.
(4) BTB Medium (L) -1 ): bromothymol blue in 1mL ethanol solution, CH 3 COONa 0.65g、NaNO 3 0.8415g、KH 2 PO 4 0.056g and agar 15 g.
(5) Aerobic Denitrification (DEM) prescreening medium (L) -1 )：CH 3 COONa 0.65g、NaNO 3 0.3415g、KH 2 PO 4 0.056g, 30g of seawater element and 2mL of trace element solution.
Example 1
Enrichment, separation, screening and identification of heterotrophic nitrification-aerobic denitrification strains
(1) Pretreatment of seabed sediments: the experimental seabed sediment is taken from yellow sea A4(122 degrees 48 'E, 35 degrees 59' N) and 10g of seabed sediment, is put into a 300mL wide-mouth triangular flask filled with 90mL of sterile physiological saline with the concentration of 0.9 percent in a super clean workbench, a little glass beads sterilized by high-pressure steam at 121 ℃ for 15min are put in the flask, and the flask is shaken at 180r/min for 1h to break up a substrate sludge sample, so that microorganisms in the substrate sludge are fully suspended in the physiological saline.
(2) Enrichment culture of strains: 20mL of the above-mentioned substrate sludge pretreatment mixture was added to a 500mL Erlenmeyer flask containing 200mL of sea LB medium and cultured in a shaker at 25 ℃ and 180r/min for 48 hours.
(3) Separating and purifying strains: taking 1mL of water samples of the upper layer, the middle layer and the lower layer of the enrichment medium respectively, respectively connecting the water samples into a centrifuge tube filled with 9mL of sterile physiological saline, repeating the step, and sequentially diluting the water samples to 10 -2 ～10 -8 A concentration gradient. Then 100L of each mixed solution with each concentration gradient is respectively taken out and respectively coated on BTB solid culture media, and the BTB solid culture media are inversely cultured in a constant temperature incubator at 25 ℃ for 2-3 days until a single colony with blue halo grows out. And (3) picking the single colony by using an inoculating loop, and repeatedly performing line drawing separation on a new LB solid culture medium for many times by adopting a plate line drawing separation method until a pure colony is obtained.
(4) Screening and identification of strains: the purified strains are selected and respectively inoculated in a heterotrophic nitrification pre-screening culture medium, and cultured for 48 hours in a constant temperature shaking table with the temperature of 25 ℃ and the rpm of 180. Taking 12h, 24h and 48h culture solution for measurementDetermining OD 600 Centrifuging at 12000rpm for 5min, collecting supernatant, and measuring NH 4 + -N、NO 2 - -N、NO 3 - -N concentration. The microorganism which has the highest ammonia nitrogen removal efficiency, the lowest accumulation of nitrite and nitrate and the self-flocculation capability is selected, namely the Pseudomonas Kunmenensis (Pseudomonas kunmingensis) 8-C provided by the invention. As shown in FIG. 1, the colony morphology of Pseudomonas (Pseudomonas kunmingens)8-C obtained in this example was: the single bacterial colony is light yellow, the surface is raised and glossy, the viscosity of the bacterial colony is small, the bacterial colony is not easy to pick, the edge is neat, and the diameter of the bacterial colony is smaller than that of other screened bacterial strains, and is about 0.5mm-1.0 mm; the Pseudomonas kunmingens 8-C is observed to be rod-shaped and slightly bent by SEM, the thallus is slender, and the length of a single cell is about 2.5 m; inputting the tested 16srDNA sequence into a GenBank database website of an NCBI website for similarity comparison, and drawing a phylogenetic tree. The phylogenetic tree results are shown in FIG. 2, and the results show that Pseudomonas kunmingens 8-C has high similarity to Pseudomonas sp.HSI-7 and Pseudomonas kunmingens strain P3-1-1, so the strain is named as Pseudomonas kunmingens 8-C. The 16srDNA sequence is as follows:
GGCGTGGGCAAAAAGCTACCTGCTAGTCGAGCGGATGAAGAGAGCTTGCTTTCTGATTCAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCCTGATAGTGGGGGACAACGTTTCGAAAGGAACGCTAATACCGCATACGTCCTACGGGAGAAAGCAGGGGACCTTCGGGCCTTGCGCTATCAGATGAGCCTAGGTCGGATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATCCGTAACTGGTCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCATTAACCTAATACGTTAGTGTTTTGACGTTACCGACAGAATAAGCACCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGAATGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATCCAAAACTGGCAAGCTAGAGTATGGCAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGGCTAATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTAGCCGTTGGGATCCTTGAGATCTTAGTGGCGCAGCTAACGCATTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGCCTTGACATGCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCTGACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTAGTTACCAGCACGTTAAGGTGGGCACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCTGGGCTACACACGTGCTACAATGGTCGGTACAAAGGGTTGCCAAGCCGCGAGGTGGAGCTAATCCCATAAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGTGAATCAGAATGTCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCTCCAGAAGTAGCTAGTCTAACATCGGGGGGCACAGTACCACTGAGGAT(SEQ ID NO.1)
example 2
Determination of ammonia nitrogen and nitrate metabolism characteristics of Pseudomonas kunmingens 8-C
Inoculating Pseudomonas (Pseudomonas kunmingens)8-C into seawater LB culture medium, and shake culturing in a shaker at 25 deg.C and 180r/min for 24h to obtain seed liquid for subsequent experiment. Centrifuging the prepared Pseudomonas kunmingens 8-C seed solution at 4000rpm for 5 min; removing supernatant, resuspending with sterilized normal saline (0.85% seawater) and washing thallus; centrifuging at 4000rpm for 5min, and removing supernatant; re-suspending the thalli by using sterilized normal saline, respectively inoculating the thalli in culture media with ammonia nitrogen and nitrate as single nitrogen sources by using 10 percent of inoculation amount, and sampling according to time points to determine the utilization and transformation of the strain on various nitrogen sources.
The culture medium used in the method is HNM culture medium and DEM culture medium.
As can be seen from figure 3, the strain can utilize ammonia nitrogen and nitrate as nitrogen sources under aerobic conditions, no nitrite nitrogen is accumulated in the ammonia nitrogen conversion process, and a small amount of nitrate is generated and then removed. As can be seen from FIG. 4, the total nitrogen in the system is converted from the water phase to the gas phase and converted into the biomass in the process of utilizing ammonia nitrogen and nitrate by the strain, and about 50 percent of the nitrogen is lost in the gas state.
Example 3
Pseudomonas kunmingens 8-C optimal growth conditions and denitrification conditions
Inoculating Pseudomonas (Pseudomonas kunmingens)8-C into seawater LB culture medium, and shake-culturing in shaker at 25 deg.C and 180r/min for 24h to obtain seed solution for subsequent experiment. Centrifuging the obtained seed liquid of Pseudomonas kunmingens 8-C at 4000rpm for 5 min; removing supernatant, resuspending with sterilized normal saline (0.85% seawater extract), and washing the thallus; centrifuging at 4000rpm for 5min, and removing supernatant; re-suspending the thalli by using sterilized normal saline, respectively inoculating the thalli into HNM culture medium by using 10 percent of inoculation amount, exploring the influence of different salinity and pH on the denitrification efficiency of the strain, and sampling according to time points to determine the utilization and the conversion of ammonia nitrogen by the strain.
The culture medium used in the method is an HNM culture medium, the simulated wastewater with different salinity is prepared by 1%, 2%, 3%, 5% and 8% (w/w) of seawater, and the simulated wastewater with different pH values is respectively prepared by (1+2) M HCl and (1+4) M NaOH.
As can be seen from fig. 5, the strain has a high tolerance to salinity conditions. Compared with low salinity, the strain has higher ammonia nitrogen removal effect under medium and high salinity. In the salinity range of 3-8%, the ammonia nitrogen removal rate exceeds 85%; the ammonia nitrogen removal rate is over 40 percent when the salinity is 1 to 2 percent.
As can be seen from figure 6, the strain has wide tolerance range on pH, is more beneficial to the growth and metabolism of the strain under neutral and weak alkaline conditions, and has ammonia nitrogen removal efficiency of over 90 percent within the range of pH 7-9.
Example 4
Pseudomonas kunmingens 8-C at Pb 2+ 、Cd 2+ Removal of ammonia nitrogen under stress
Inoculating Pseudomonas (Pseudomonas kunmingens)8-C into seawater LB culture medium, and shake culturing in a shaker at 25 deg.C and 180r/min for 24h to obtain seed liquid for subsequent experiment. Centrifuging the obtained Pseudomonas kunmingens 8-C seed liquid at 4000rpm for 5 min; removing supernatant, resuspending with sterilized normal saline (0.85% seawater extract), and washing the thallus; centrifuging at 4000rpm for 5min, and removing supernatant; resuspending the strain again with sterilized normal saline, inoculating 10% of the strain in HNM medium, and exploring the strain containing Pb 2+ 、Cd 2+ And (3) measuring the denitrification capability of the strain in the wastewater according to time point sampling to determine the utilization of ammonia nitrogen by the strain.
As can be seen from FIG. 7, Pseudomonas kunmingens 8-C is responsible for Pb 2+ 、Cd 2+ Has better tolerance, 20mg/L of Pb 2+ The removal rate of ammonia nitrogen under stress can reach 85 percent, and the removal rate is 10mg/L Cd 2+ The ammonia nitrogen removal rate can reach 80 percent under stress.
Example 5
Pseudomonas kunmingens (Pseudomonas kunmingens)8-C and Acinetobacter (Acinetobacter johnsonii)2-1-H construct communities
A method for the development of a salt nitrogen heterotrophic nitrification-aerobic denitrification microbiome by Pseudomonas (Pseudomonas kunmingens)8-C and another strain of HNAD Acinetobacter (Acinetobacter johnsonii)2-1-H comprises the following steps:
(1) respectively inoculating Pseudomonas (Pseudomonas kunmingens 8-C) and Acinetobacter (Acinetobacter johnsonii2-1-H) into seawater LB culture medium, and performing shake culture in a shaking table at 25 ℃ and 180r/min for 24H to obtain activated bacteria liquid;
(2) mixing Pseudomonas (Pseudomonas kunmingens)8-C and Acinetobacter (Acinetobacter johnsonii)2-1-H thallus prepared in the step (1) according to the volume ratio of 1: the proportion of 1 is inoculated in a sequencing batch bioreactor (SBR), aeration is carried out for 24 hours, then sedimentation is carried out for 12 hours, supernatant is discarded, the same volume of simulated wastewater is added, and the step is repeated until LB is completely replaced. The sequencing batch bioreactor (SBR) has an effective volume of 3.2L, and an air diffusion device is arranged at the bottom of the SBR to play roles in aeration and stirring. The reactor was operated in a continuous mode with each cycle comprising 5min of water inlet, 450min of aeration, 15min of settling and 10min of water outlet, each cycle for 8h, a volume exchange rate of 62.5% and a Hydraulic Retention Time (HRT) of 12.8 h. The sequencing batch bioreactor operating parameters are shown in table 1.
TABLE 1
The components of the seawater simulated wastewater in the step (2) are shown in table 2:
TABLE 2
Sludge is not discharged during the operation of the reactor, so that a salt-adaptive heterotrophic nitrification-aerobic denitrification microbial community with stable ammonia nitrogen removal efficiency and good sedimentation performance is obtained, and the total nitrogen, ammonia nitrogen, nitrite and nitrate concentration is measured by sampling at regular time. The operation results are shown in fig. 8, and it can be seen from the results that in the whole operation process, the heterotrophic nitrification-aerobic denitrification microbiome which is synthesized and developed by Pseudomonas (Pseudomonas kunmingens)8-C and Acinetobacter (Acinetobacter johnsonii)2-1-H has good and stable treatment effect, the ammonia nitrogen removal rate in the simulated high-salt wastewater can reach 90%, the total nitrogen removal rate can reach 85%, and almost no nitrite and nitrate are accumulated. The reactor continuously operates for 40 days, the biomass growth rate is low, the sludge yield is small, the sludge discharge frequency is reduced, the energy and resource consumption in the subsequent sludge reduction treatment is reduced, and a method is provided for treating the high-salinity wastewater containing heavy metal.
It should be noted that the above-mentioned embodiments are only preferred embodiments of the present invention, and the present invention is not limited thereto, and although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and equivalents can be made in the technical solutions described in the foregoing embodiments, or equivalents thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. Although the present invention has been described with reference to the specific embodiments, it should be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention.
SEQUENCE LISTING
<110> Shandong university
<120> a highly salt-tolerant and heavy metal-tolerant heterotrophic nitrification-aerobic denitrification self-flocculation marine bacterium and community structure thereof
Application in construction
<130>
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1435
<212> DNA
<213> Pseudomonas kunmingensis kunming genes
<400> 1
ggcgtgggca aaaagctacc tgctagtcga gcggatgaag agagcttgct ttctgattca 60
gcggcggacg ggtgagtaat gcctaggaat ctgcctgata gtgggggaca acgtttcgaa 120
aggaacgcta ataccgcata cgtcctacgg gagaaagcag gggaccttcg ggccttgcgc 180
tatcagatga gcctaggtcg gattagctag ttggtgaggt aacggctcac caaggcgacg 240
atccgtaact ggtctgagag gatgatcagt cacactggaa ctgagacacg gtccagactc 300
ctacgggagg cagcagtggg gaatattgga caatgggcga aagcctgatc cagccatgcc 360
gcgtgtgtga agaaggtctt cggattgtaa agcactttaa gttgggagga agggcattaa 420
cctaatacgt tagtgttttg acgttaccga cagaataagc accggctaac ttcgtgccag 480
cagccgcggt aatacgaagg gtgcaagcgt taatcggaat tactgggcgt aaagcgcgcg 540
taggtggttt gttaagttga atgtgaaagc cccgggctca acctgggaac tgcatccaaa 600
actggcaagc tagagtatgg cagagggtgg tggaatttcc tgtgtagcgg tgaaatgcgt 660
agatatagga aggaacacca gtggcgaagg cgaccacctg ggctaatact gacactgagg 720
tgcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc gtaaacgatg 780
tcgactagcc gttgggatcc ttgagatctt agtggcgcag ctaacgcatt aagtcgaccg 840
cctggggagt acggccgcaa ggttaaaact caaatgaatt gacgggggcc cgcacaagcg 900
gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggcct tgacatgcag 960
agaactttcc agagatggat tggtgccttc gggaactctg acacaggtgc tgcatggctg 1020
tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgta acgagcgcaa cccttgtcct 1080
tagttaccag cacgttaagg tgggcactct aaggagactg ccggtgacaa accggaggaa 1140
ggtggggatg acgtcaagtc atcatggccc ttacggcctg ggctacacac gtgctacaat 1200
ggtcggtaca aagggttgcc aagccgcgag gtggagctaa tcccataaaa ccgatcgtag 1260
tccggatcgc agtctgcaac tcgactgcgt gaagtcggaa tcgctagtaa tcgtgaatca 1320
gaatgtcacg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccatgggagt 1380
gggttgctcc agaagtagct agtctaacat cggggggcac agtaccactg aggat 1435
Claims (10)
1. A heterotrophic nitrification-aerobic denitrification strain is characterized by being classified and named as Kunming pseudomonads (Pseudomonas kunmingens)8-C, and the strain is preserved in China center for type culture Collection (CCTCC NO) 12 and 13 months in 2021, and the biological preservation number is CCTCC NO: m20211592.
2. A microbial inoculant comprising the heterotrophic nitrification-aerobic denitrification bacterium 8-C of claim 1.
3. The microbial inoculant according to claim 2, further comprising a carrier; preferably, the carrier is a carrier which is commonly used in the field of microbial agents and is inert in biology;
preferably, the carrier is a solid carrier or a liquid carrier.
4. A heterotrophic nitrification-aerobic denitrification microbiome suitable for salt, which comprises the heterotrophic nitrification-aerobic denitrification bacteria 8-C of claim 1;
preferably, the halophilic heterotrophic nitrification-aerobic denitrification microbiome is obtained by inoculating the heterotrophic nitrification-aerobic denitrification bacteria 8-C and Acinetobacter johnsonii (Acinetobacter johnsonii)2-1-H into high-salt wastewater for culture;
wherein, the Acinetobacter johnsonii (Acinetobacter johnsonii)2-1-H is preserved in China center for type culture Collection at 12 months and 13 months in 2021, and the biological preservation number is CCTCC NO: m20211593;
preferably, the salt-nitrogen-assimilating microorganism group is high-salt wastewater obtained after the treatment or activated sludge obtained after the treatment.
5. The method for constructing a saltcompatible nitrogen assimilating microorganism according to claim 4, characterized in that it comprises the following steps:
inoculating the heterotrophic nitrification-aerobic denitrification bacteria 8-C and the Acinetobacter johnsonii2-1-H into a bioreactor containing high-salinity wastewater, and operating until the ammonia nitrogen removal efficiency and the sedimentation performance are stabilized, so as to obtain the salt-adapted heterotrophic nitrification-aerobic denitrification microorganism group.
6. The construction method according to claim 5, wherein the high-salinity wastewater comprises actual high-salinity wastewater and simulated high-salinity wastewater;
preferably, the simulated high-salinity wastewater is seawater simulated wastewater, and comprises the following specific components: 3% of seawater element, 500mg/L of sodium acetate, 140mg/L of dipotassium phosphate and 550mg/L of ammonium chloride.
7. The method of claim 5, wherein the bioreactor is a sequencing batch bioreactor, and preferably the method comprises:
the continuous operation is adopted, each cycle comprises 5min water inlet, 450min aeration, 15min sedimentation and 10min water outlet, each period is 8h, the volume exchange rate is 62.5%, and the hydraulic retention time is 12.8 h.
8. Use of the heterotrophic nitrification-aerobic denitrification bacteria of claim 1, the microbial inoculant of claim 2 and/or the saltaccommodating heterotrophic nitrification-aerobic denitrification microbiome of claim 4 in any one or more of the following:
a) treating wastewater;
b) preparing an organic fertilizer;
c) soil improvement;
d) treating water eutrophication;
e) repairing water body pollution;
f) and (4) biological denitrification.
9. The use of claim 8, wherein in a), the wastewater comprises high-salinity wastewater, mariculture wastewater, industrial saline wastewater and seawater toilet wastewater; the wastewater also comprises heavy metal polluted wastewater, and the heavy metals comprise lead and cadmium;
in said c), said soil comprises saline alkali soil; the soil comprises a heavy metal contaminated saline-alkali earth, the heavy metal comprising lead and cadmium; preferably, the soil improvement is soil fertility improvement;
in d), the body of water comprises fresh water and seawater, preferably seawater.
10. A method for the integrated resource conversion of nutrients in a high-salt environment is characterized by comprising the following steps: applying the heterotrophic nitrification-aerobic denitrification bacteria of claim 1, the microbial inoculant of claim 2 and/or the saltaccommodating heterotrophic nitrification-aerobic denitrification microbiome of claim 4 to a high salinity environment;
preferably, the high-salt environment is a high-salt water environment, and further comprises a high-salt water environment polluted by heavy metals; the salinity of the high-salinity water environment is not lower than 3 percent, and the salinity is more preferably 3 to 8 percent; the heavy metals include lead and cadmium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210562929.8A CN115125164B (en) | 2022-05-23 | 2022-05-23 | Highly salt-resistant and heavy metal-resistant heterotrophic nitrification-aerobic denitrification self-flocculation marine bacterium and application thereof in community construction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210562929.8A CN115125164B (en) | 2022-05-23 | 2022-05-23 | Highly salt-resistant and heavy metal-resistant heterotrophic nitrification-aerobic denitrification self-flocculation marine bacterium and application thereof in community construction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115125164A true CN115125164A (en) | 2022-09-30 |
| CN115125164B CN115125164B (en) | 2024-05-10 |
Family
ID=83375897
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210562929.8A Active CN115125164B (en) | 2022-05-23 | 2022-05-23 | Highly salt-resistant and heavy metal-resistant heterotrophic nitrification-aerobic denitrification self-flocculation marine bacterium and application thereof in community construction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115125164B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115011522A (en) * | 2022-06-17 | 2022-09-06 | 贵州大学 | EN-J1 denitrifying bacteria and application thereof in nitrogen-containing sewage treatment |
| CN116179434A (en) * | 2023-01-28 | 2023-05-30 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Efficient and stable construction method and application of sulphur oxidation denitrification engineering microorganism group |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110217895A (en) * | 2019-05-31 | 2019-09-10 | 中国科学院微生物研究所 | A kind of complex micro organism fungicide and its application for water environment treatment |
| CN110655197A (en) * | 2018-06-29 | 2020-01-07 | 龙岩学院 | Method for treating nitrate nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification pseudomonas strain |
| US20210196828A1 (en) * | 2019-12-30 | 2021-07-01 | Eligo Bioscience | Bacterial delivery vehicles for in vivo delivery of a dna payload |
| CN113403234A (en) * | 2021-02-01 | 2021-09-17 | 山东大学 | Marine self-flocculating bacterium and halophilic nitrogen assimilation microorganism group driven to develop by marine self-flocculating bacterium as well as construction method and application of marine self-flocculating bacterium and halophilic nitrogen assimilation microorganism group |
| CN113684154A (en) * | 2021-09-08 | 2021-11-23 | 青岛蔚蓝赛德生物科技有限公司 | Pseudomonas Kunmingensis strain and application thereof in environment-friendly water treatment |
| US20220135984A1 (en) * | 2019-12-30 | 2022-05-05 | Eligo Bioscience | Microbiome modulation of a host by delivery of dna payloads with minimized spread |
-
2022
- 2022-05-23 CN CN202210562929.8A patent/CN115125164B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110655197A (en) * | 2018-06-29 | 2020-01-07 | 龙岩学院 | Method for treating nitrate nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification pseudomonas strain |
| CN110217895A (en) * | 2019-05-31 | 2019-09-10 | 中国科学院微生物研究所 | A kind of complex micro organism fungicide and its application for water environment treatment |
| US20210196828A1 (en) * | 2019-12-30 | 2021-07-01 | Eligo Bioscience | Bacterial delivery vehicles for in vivo delivery of a dna payload |
| US20220135984A1 (en) * | 2019-12-30 | 2022-05-05 | Eligo Bioscience | Microbiome modulation of a host by delivery of dna payloads with minimized spread |
| CN113403234A (en) * | 2021-02-01 | 2021-09-17 | 山东大学 | Marine self-flocculating bacterium and halophilic nitrogen assimilation microorganism group driven to develop by marine self-flocculating bacterium as well as construction method and application of marine self-flocculating bacterium and halophilic nitrogen assimilation microorganism group |
| CN113684154A (en) * | 2021-09-08 | 2021-11-23 | 青岛蔚蓝赛德生物科技有限公司 | Pseudomonas Kunmingensis strain and application thereof in environment-friendly water treatment |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115011522A (en) * | 2022-06-17 | 2022-09-06 | 贵州大学 | EN-J1 denitrifying bacteria and application thereof in nitrogen-containing sewage treatment |
| CN115011522B (en) * | 2022-06-17 | 2023-05-12 | 贵州大学 | EN-J1 denitrifying bacteria and application thereof in nitrogen-containing sewage treatment |
| CN116179434A (en) * | 2023-01-28 | 2023-05-30 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Efficient and stable construction method and application of sulphur oxidation denitrification engineering microorganism group |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115125164B (en) | 2024-05-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106754570B (en) | One plant of Pseudomonas stutzeri and its application | |
| CN115125164B (en) | Highly salt-resistant and heavy metal-resistant heterotrophic nitrification-aerobic denitrification self-flocculation marine bacterium and application thereof in community construction | |
| CN103074277B (en) | Denitrifying bacterium and application thereof | |
| CN105861359A (en) | Heterotrophic nitrification-aerobic denitrification high temperature resisting strain for producing floc, and application thereof | |
| CN102443558B (en) | Composite heterotrophic nitrifying bacterial agent and application of same in nitrogen removal treatment of waste water containing ammonia and nitrogen | |
| CN113174345A (en) | Heterotrophic nitrification-aerobic denitrification strain for efficient denitrification and application thereof | |
| CN113604379B (en) | Pseudomonas holothurians with heterotrophic nitrification-aerobic denitrification function and application thereof | |
| CN107988125B (en) | Low-temperature-resistant nitrifying bacteria and application thereof | |
| CN113249254A (en) | Pseudomonas nitroreducens strain and application thereof | |
| CN114703095A (en) | Pseudomonas mendocina and application thereof in field of sewage and wastewater purification | |
| CN111057664B (en) | Novel salt-tolerant denitrifying bacterium and application thereof | |
| CN113403234B (en) | Marine self-flocculating bacterium and halophilic nitrogen assimilation microorganism group driven to develop by marine self-flocculating bacterium as well as construction method and application of marine self-flocculating bacterium and halophilic nitrogen assimilation microorganism group | |
| CN101811779B (en) | Preparation method of halophilic decontamination bacterial agent and bacterial agent prepared by same | |
| CN114908016A (en) | Pseudomonas denitrificans and application thereof in field of sewage and wastewater purification | |
| CN102690765A (en) | Low-temperature aerobic denitrifying strain Pseudomonas psychrophila Den-03 and screening method and application thereof | |
| CN113151063B (en) | Citrobacter freundii AS11 and application thereof in sewage treatment | |
| CN111471611B (en) | Rhodococcus ruber HDRR1 for purifying inorganic nitrogen and phosphorus in tail water of seawater pond culture and application thereof | |
| CN115386520B (en) | Rhodococcus pyridine-philic RL-GZ01 strain and application thereof | |
| CN114292798B (en) | Anaerobic denitrifying strain and application thereof in riverway water body remediation | |
| CN116606756A (en) | Application of high-efficiency aerobic denitrifying actinomycete strain in micro-polluted water treatment | |
| CN106434424B (en) | Vibrios and application thereof with dirty seawater denitrification ability | |
| CN113293111B (en) | Bacillus marinus with denitrification function and application thereof | |
| CN113005062B (en) | Facultative ammonia oxidizing bacteria and application thereof | |
| CN115057523A (en) | SBR wastewater treatment device and treatment method thereof | |
| CN111471612B (en) | Rhodococcus ruber HDRR2Y for purifying inorganic nitrogen and phosphorus in seawater pond culture tail water and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |