CN1151223C

CN1151223C - Hydrolytic collagen

Info

Publication number: CN1151223C
Application number: CNB01113125XA
Authority: CN
Inventors: 陈经国
Original assignee: Individual
Current assignee: Individual
Priority date: 2001-06-26
Filing date: 2001-06-26
Publication date: 2004-05-26
Anticipated expiration: 2021-06-26
Also published as: CN1328100A

Abstract

The present invention relates to hydrolytic collagen. The hydrolytic collagen is prepared in a mode that the skin of a discarded black scraper is mixed with auxiliary materials, such as distilled water, colorless edible hydrochloric acid with the content of 31%, 767 type medical carbon powder, industry ammonia water above 25%, edible sodium hydroxide above 99%, strong acid type cationic resin, acetone, ethanol, etc.; the obtained mixture is prepared into a natural compound amino acid product by the technologies, such as the soaking of a hydrochlric solution, hydrolyzation, decompression for deacidification, decolorization, filtration, column exchange, acid washing, collection, decolorization, filtration, decompression and concentration, vacuum drying, etc. A product of the present invention contains hydroxyproline benefit to human bodies, and the amino acid content is above 90%; therefore, compared with similar products, the present invention has superior quality and low cost.

Description

Hydrolytic collagen

Technical field

The present invention relates to a kind of natural compound amino-acid---manufacturing process of hydrolytic collagen and products thereof.

Background technology

On domestic and international market and the pertinent literature, do not see product and the production technique thereof of making natural compound amino-acid with waste black scraper fish-skin as yet at present.

Summary of the invention

The objective of the invention is to utilize waste black scraper Puffer fish-skin and relevant auxiliary material, production technique of manufacturing natural compound amino-acid---hydrolytic collagen and products thereof.

Hydrolytic collagen of the present invention, by black scraper Puffer fish-skin through the salt acid soak, pyrohydrolysis, medicinal carbon dust decolouring, the strong acid type cationic resin purifying, add acetone, carrying out vacuum-drying behind the ethanol makes, its manufacturing process flow is: fish-skin → about 8 hours → cleaning of immersion drying in the PH2-3 aqueous hydrochloric acid → in the hydrochloric acid soln of concentration 6-10MoI, temperature is that 100 ℃ of-110 ℃ of scopes are carried out hydrolysis in 8-16 hour → at vacuum tightness 0.06Mpa, reduce pressure under temperature 65-75 ℃ of state deacidify → press the fish-skin amount 5%, add activated carbon, under 70 ℃ of conditions of temperature, keep half an hour, decolour processing → filtration → filtrate → carried out upper prop exchange 2 hours → wash acid treatment → usefulness 1.5N ammoniacal liquor wash-out with distilled water with the strong acid type cationic resin of handling, from PH=4, to PH=8, carry out collection and treatment → press 3% of fish-skin amount, add carbon dust, decolour → filter → under vacuum tightness 0.06Mpa, heating 60 ℃-65 ℃ at normal temperatures under the vacuum tightness 0.06Mpa and carrying out the concentrating under reduced pressure processing, after becoming off-white color pasty state xln → and adding ethanol in the 10-15% ratio of fish-skin amount, become faint yellow meal → at vacuum tightness 0.06Mpa, under 70 ℃ of conditions, carry out vacuum-drying → product;

The treatment process of described strong acid type cationic resin is: strong acid type cationic resin → soak half an hour in 4% sodium hydroxide solution, water is flushed to PH=7 → in the hydrochloric acid soln of PH=1 and soaked 8 hours then, uses distilled water flushing PH=5-6 then;

Described to wash sour technology be to be flushed to PH4-5 and to test with silver nitrate solution, until not showing that the chlorion reaction just stops to wash.

Advantage of the present invention is, the natural compound amino-acid hydrolytic collagen that the present invention makes, with domestic like product, compare as hydrolyzed animal protein, hydrolyzed vegetable protein, product of the present invention contains the oxyproline very useful to human body, and the total aminoacids content of the present invention is up to more than 90%, so, compared with similar products, quality is superior, and cost is low.

Embodiment

Embodiment:

Dry, after pure fish-skin weighs, immersion was carried out decalcification processing → cleaning drying back in about 8 hours in the beneficial acid solution of concentration 6-8M01 in the aqueous hydrochloric acid of PH2-3, temperature is that 100 ℃ of-110 ℃ of scopes are carried out hydrolysis in 8-16 hour, hydrochloric acid is food grade more than 99% → at vacuum tightness 0.06Mpa, reduce pressure except that acid treatment under 65 ℃ of-70 ℃ of states of temperature, this processing need be carried out 20% of 2-3 time → dry fish-skin weight, add activated carbon, under 70 ℃ of conditions of temperature, keep half an hour, decolour processing → filtration → filtrate → carried out upper prop exchange 2 hours → wash acid treatment → with carrying out collection and treatment behind 1.5N ammoniacal liquor (technical grade is more than the 25%) wash-out with distilled water with the strong acid type cationic resin of handling, by 3% of dry fish-skin amount, add medicinal carbon dust 767 types, decolour at normal temperatures → under vacuum tightness 0.06Mpa, heat 60 ℃-65 ℃ and carry out concentrating under reduced pressure and handle, after becoming off-white color pasty state xln → and adding ethanol in the 10-15% ratio of fish-skin amount, become faint yellow meal → at vacuum tightness 0.06Mpa, under 70 ℃ of conditions, carry out vacuum-drying → product.

Claims

1, a kind of hydrolytic collagen, it is characterized in that: this hydrolytic collagen, by black scraper Puffer fish-skin through the salt acid soak, pyrohydrolysis, medicinal carbon dust decolouring, the strong acid type cationic resin purifying, add acetone, carrying out vacuum-drying behind the ethanol makes, its manufacturing process flow is: fish-skin → about 8 hours → cleaning of immersion drying in the PH2-3 aqueous hydrochloric acid → in the hydrochloric acid soln of concentration 6-10M, temperature is that 100 ℃ of-110 ℃ of scopes are carried out hydrolysis in 8-16 hour → at vacuum tightness 0.06Mpa, reduce pressure under the temperature 65-75 ℃ of state and deacidify → press 5% of fish-skin amount, add activated carbon, under 70 ℃ of conditions of temperature, keep half an hour, decolour processing → filtration → filtrate → carried out upper prop exchange 2 hours → wash acid treatment → usefulness 1.5N ammoniacal liquor wash-out with distilled water with the strong acid type cationic resin of handling, from PH=4, to PH=8, carry out collection and treatment → press 3% of fish-skin amount, add carbon dust, decolour → filter → under vacuum tightness 0.06Mpa, heating 60 ℃-65 ℃ at normal temperatures under the vacuum tightness 0.06Mpa and carrying out the concentrating under reduced pressure processing, after becoming off-white color pasty state xln → and adding ethanol in the 10-15% ratio of fish-skin amount, become faint yellow meal → at vacuum tightness 0.06Mpa, under 70 ℃ of conditions, carry out vacuum-drying → product.

2, by the described hydrolytic collagen of claim 1, it is characterized in that: the treatment process of described strong acid type cationic resin is: strong acid type cationic resin → soak half an hour in 4% sodium hydroxide solution, water is flushed to PH=7 → in the hydrochloric acid soln of PH=1 and soaked 8 hours then, uses distilled water flushing PH=5-6 then.

3, by the described hydrolytic collagen of claim 1, it is characterized in that: described to wash sour technology be to be flushed to PH4-5 and to test with silver nitrate solution, until not showing that the chlorion reaction just stops to wash.