CN115109144A - Preparation method of bioactive collagen peptide and bioactive collagen peptide - Google Patents
Preparation method of bioactive collagen peptide and bioactive collagen peptide Download PDFInfo
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- CN115109144A CN115109144A CN202210765908.6A CN202210765908A CN115109144A CN 115109144 A CN115109144 A CN 115109144A CN 202210765908 A CN202210765908 A CN 202210765908A CN 115109144 A CN115109144 A CN 115109144A
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- 230000000975 bioactive effect Effects 0.000 title claims abstract description 118
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 48
- 238000003756 stirring Methods 0.000 claims description 41
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 38
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- 238000000034 method Methods 0.000 claims description 19
- ILXAOQAXSHVHTM-UHFFFAOYSA-M sodium;2-amino-2-(hydroxymethyl)propane-1,3-diol;chloride Chemical compound [Na+].[Cl-].OCC(N)(CO)CO ILXAOQAXSHVHTM-UHFFFAOYSA-M 0.000 claims description 19
- 241000283707 Capra Species 0.000 claims description 18
- 238000000502 dialysis Methods 0.000 claims description 18
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- 108091005658 Basic proteases Proteins 0.000 claims description 9
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- 102000004139 alpha-Amylases Human genes 0.000 claims description 9
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- 229940024171 alpha-amylase Drugs 0.000 claims description 9
- SOQBVABWOPYFQZ-UHFFFAOYSA-N oxygen(2-);titanium(4+) Chemical compound [O-2].[O-2].[Ti+4] SOQBVABWOPYFQZ-UHFFFAOYSA-N 0.000 claims description 9
- 229940055729 papain Drugs 0.000 claims description 9
- 235000019834 papain Nutrition 0.000 claims description 9
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- 238000006460 hydrolysis reaction Methods 0.000 claims description 8
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- 235000015278 beef Nutrition 0.000 description 11
- 239000011734 sodium Substances 0.000 description 8
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- 239000008215 water for injection Substances 0.000 description 5
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- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
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- 239000012567 medical material Substances 0.000 description 1
- WVJKHCGMRZGIJH-UHFFFAOYSA-N methanetriamine Chemical compound NC(N)N WVJKHCGMRZGIJH-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- General Health & Medical Sciences (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
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Abstract
The invention discloses a preparation method of bioactive collagen peptide and the bioactive collagen peptide. S1, using any one or more of purified animal dermis skin sheet, purified animal tendon membrane sheet and purified animal tendon particles as raw materials, sequentially weighing, shearing and/or crushing, softening, controlling water, fluffing, adjusting pH, homogenizing, dialyzing, and freeze-drying to obtain fluffy and porous high-level collagen aggregate; and S2, sequentially carrying out ultrasonic treatment, enzymolysis, cooling, centrifugation, membrane concentration, spray drying, packaging and sterilization on the high-level collagen aggregate obtained in the step S1 to obtain a finished product of the bioactive collagen peptide. The bioactive collagen peptide has excellent quality, completely eliminates antigen substances, has excellent biocompatibility and good bioactivity, can induce cell growth and tissue regeneration, and can be widely used in the fields of polypeptide drug research and development, clinical medical treatment, food health care, medical cosmetology, skin care, skin beautification and the like.
Description
Technical Field
The invention relates to the technical field of preparation of medical collagen peptide, in particular to a preparation method of bioactive collagen peptide and the bioactive collagen peptide.
Background
Collagen is a protein having a natural triple helix structure and having good biological activity, and has been widely used in the fields of biomedicine and medical cosmetology. Collagen peptides are hydrolysates of collagen molecules having two or more amino acid residues. Collagen peptides can be classified into oligo-and polypeptides, depending on the number of residues contained therein. In general, collagen peptides having 10 or more amino acid residues are called "polypeptides", and collagen peptides having 10 or less amino acid residues are called "oligopeptides". It was found that due to its specific structure, collagen peptide is no longer bound by a large number of hydrogen bonds and secondary bonds, and its diverse behavior in terms of amino acid type, sequence and spatial structure determines its diversity in properties. With the continuous development and progress of science and technology, the research of human beings on collagen peptide is continuous and deep, the medical, health care and beauty functions and the medicinal function of collagen peptide are gradually favored by people, and the collagen peptide and the application thereof become a hot spot.
A large number of studies have shown that collagen peptide has healing promoting activity, antioxidant activity and antihypertensive activity, and these excellent properties of collagen peptide have been widely applied to the fields of polypeptide drug development, clinical medicine, food health, medical beauty, skin care and beauty, and the like, and are widely appreciated by consumers. Therefore, the medical collagen peptide is stably produced in a large scale with high quality, and the market prospect is very wide.
As is well known, collagen is finely treated by physical, chemical or biological means and is hydrolyzed into highly hydrophilic compounds of relatively low molecular weight, i.e., collagen peptides. The collagen peptide is prepared mainly by enzymolysis, chemical method, physical method, etc. The enzyme binding method is now generally used. The existing enzyme-enzyme combination method is more effective and simple and feasible compared with the traditional method, and can improve the quality of the collagen polypeptide. But the process control difficulty is large, and the biocompatibility and the bioactivity of the obtained collagen polypeptide still cannot meet the medical requirements.
The applicant has found that the prior art has at least the following technical problems:
1. the method for preparing the collagen peptide in the prior art can not thoroughly eliminate the antigen substance in the collagen peptide, and has the risk of rejection reaction in clinical use;
2. the collagen peptide prepared by the prior art has low bioactivity and poor capability of inducing and promoting the growth of tissues and cells;
3. the process controllability of the prior art is poor, the quality of the prepared collagen peptide is unstable, and the high-quality and large-scale stable production is difficult to realize.
Disclosure of Invention
The invention aims to provide a preparation method of bioactive collagen peptide and the bioactive collagen peptide, and aims to solve the technical problems that the method for preparing the collagen peptide in the prior art cannot completely remove antigen substances in the collagen peptide, and rejection reaction risks occur in clinical use.
In order to achieve the purpose, the invention provides the following technical scheme:
the invention provides a preparation method of bioactive collagen peptide, which comprises the following steps:
s1, taking any one or more of a purified animal dermal skin sheet, a purified animal tendon membrane sheet and a purified animal tendon particle as a raw material, sequentially weighing, shearing and/or crushing, softening, controlling water, fluffing, adjusting pH, homogenizing, dialyzing, and freeze-drying to obtain a fluffy and porous high-level collagen aggregate;
and S2, sequentially carrying out ultrasonic treatment, enzymolysis, cooling, centrifugation, membrane concentration, spray drying, packaging and sterilization on the high-level collagen aggregate obtained in the step S1 to obtain a finished product of the bioactive collagen peptide.
Further, in step S1, the specific preparation steps of the high-level collagen aggregate are:
s11, weighing
Weighing the selected raw materials;
s12, cutting or crushing
When the selected raw materials comprise purified animal dermis sheets, cutting the purified animal dermis sheets into small pieces to obtain small pieces of sheets; when the selected raw materials comprise purified animal tendon diaphragms or purified animal tendon particles, the purified animal tendon diaphragms or the purified animal tendon particles are crushed to obtain finer particles;
s13 softening
Placing the small leather sheets or particles obtained in the step S12 into a low-temperature rotary drum, and adding normal saline for soaking, wherein the soaking is divided into two stages: the first stage soaking is to stand and soak at 3.0-5.0 deg.C for 30-90 min; the second stage of soaking is soaking for 180 min under the condition of intermittent stirring at the temperature of 3.0-5.0 ℃;
s14, controlling water
Draining the waste liquid soaked in the low-temperature rotary drum;
s15, puffing
Adding Tris-NaCl buffer solution into the low-temperature rotary drum for soaking, wherein the soaking is divided into two stages: the first stage of soaking is to stand and soak for 110-; the second stage of soaking is to soak for 180-; when the selected raw materials comprise purified animal tendon membranes or purified animal tendon particles, the soaking is finished until fine particles can be obviously observed to suspend; then removing clear liquid;
s16, adjusting pH
Adding an acetic acid solution into the low-temperature rotary drum, adjusting the pH to 2-2.5, and mixing the solution in a ratio of 1: 48-52; then standing and soaking for 1.5-2.5h until the small leather sheets and/or the particles are transparent;
s17 homogenate
Adding acetic acid solution into the low-temperature rotary drum, and uniformly stirring; then adding NaOH solution into the low-temperature rotary drum to adjust the pH value to 6.5-7.5; adding NaCl with final concentration of 1.40-1.60M, and salting out for 22-26h until white flocculent separation appears; centrifuging at 3-5 deg.C and 8000-; eluting the precipitate with acetic acid solution, and stirring at 3-5 deg.C to obtain high-level collagen aggregate dispersion;
s18, dialysis
Pouring the dispersion liquid of high-level collagen aggregate into dialysis bag with dialysis molecular weight of 1k-4k, 0.03-0.05mol/L and 0.01-0.03mol/L Na 2 HPO 4 Dialyzing the solution for 1.5-2.5 days, and dialyzing the solution for 1.5-2.5 days by using distilled water;
s19, freeze drying
And (3) uniformly transferring the dialyzed high-level collagen aggregate into a glass dish, freezing and drying to obtain a fluffy and porous high-level collagen aggregate, and sealing, drying and storing for later use.
Further, in the step S12, the small leather sheets are rectangular, and have a length of 2-3cm and a width of 2-3 cm; when the selected raw materials comprise purified animal tendon particles, the diameter of the purified animal tendon particles is 1.0-1.5cm, and the diameter of the purified animal tendon membrane or the finer particles obtained after the purified animal tendon membrane or the purified animal tendon particles are crushed is 0.5-0.8 cm.
In the step S13, the intermittent stirring is performed by rotating for 5-10min every 50-55min and repeating for 3-5 times; the addition amount of the normal saline is 2-3 times of the total weight of the raw materials;
in the step S15, the intermittent stirring is performed by rotating for 5-10min every 50-55min and repeating for 3-6 times;
in step S15, the amount of Tris-NaCl buffer solution is 10mM, and the addition amount is 12-25 times of the total weight of the raw materials.
Further, in the S16, the acetic acid solution is 0.45-0.55M, and the addition amount is 2-3 times of the total weight of the raw materials;
in the step S17, the acetic acid solution is 0.45-0.55M, and the addition amount is 2-3 times of the total weight of the raw materials.
Further, in step S2, the preparation of the finished bioactive collagen peptide product includes:
s21 ultrasonic treatment
Placing the high-level collagen aggregate prepared in the step S1 in an ultrasonic rotary drum, adding distilled water into the ultrasonic rotary drum, starting the ultrasonic rotary drum, and rotating for 20-40 min; then heating to 36-52 ℃ and preserving heat for 4-8 min;
s22, enzymolysis
Adding NaOH or HCl solution into the ultrasonic rotary drum, and adjusting the pH value of the solution to 5.5-8.5; then, adding a complex enzyme preparation, and starting the ultrasonic rotary drum to rotate for 300-1200 min; heating to 95-100 deg.C, maintaining the temperature for 5-10min to inactivate enzyme, and terminating hydrolysis reaction to obtain bioactive collagen peptide solution A;
s23, cooling
Rapidly cooling the bioactive collagen peptide solution A to room temperature;
s24 centrifugation
Placing the bioactive collagen peptide liquid A cooled to room temperature in a high-speed centrifuge, centrifuging for 10-20min at 3500-4500r/min, and taking the supernatant to obtain bioactive collagen peptide liquid B;
s25, membrane concentration
Separating and concentrating the bioactive collagen peptide liquid B by adopting a nanofiltration membrane to obtain a bioactive collagen peptide liquid C;
s26 spray drying
Carrying out spray drying on the bioactive collagen peptide liquid C on a spray dryer to obtain bioactive collagen peptide powder;
s27, packaging
The packaging is carried out in a hundred-grade workshop and is divided into inner packaging and outer packaging; the inner package is divided into an inner layer and an outer layer, the inner layer is a plastic film, and the outer layer is a tin foil bag; the outer package is a hard paper bag; firstly, carrying out inner layer packaging on a powder packaging machine, and then carrying out outer layer packaging and outer packaging on a clean workbench; finally, boxing is carried out according to the specification requirement;
s28, sterilization
Sterilizing the packaged bioactive collagen peptide powder, and sterilizing by using gamma rays generated by 10.0-30.0KGy/h60Co to obtain the bioactive collagen peptide finished product.
Further, in the step S21, the amount of the distilled water added is 2-3 times of the weight of the high-level collagen aggregate;
in step S26, the spray drying conditions are: the inlet temperature is 103-107 ℃ and the outlet temperature is 55-65 ℃.
Further, in the step S22, the NaOH solution or the HCl solution is 0.9 to 1.1 mol/L; the weight ratio of the added amount of the complex enzyme preparation to the high-level collagen aggregate is 6-12: 100; the preparation of the compound enzyme preparation comprises the following raw materials in parts by weight: 50-60 parts of papain, 20-30 parts of trypsin, 8-12 parts of alkaline protease, 4-6 parts of alpha-amylase and 4-6 parts of nano titanium dioxide.
Further, in the step S22, the preparation of the complex enzyme preparation comprises the following raw materials in parts by weight: 55 parts of papain, 25 parts of trypsin, 10 parts of alkaline protease, 5 parts of alpha-amylase and 5 parts of nano titanium dioxide.
Further, in the step S1, the purified animal dermis skin sheet is any one of a purified pig dermis skin sheet, a purified fetal bovine dermis skin sheet, and a purified goat dermis skin sheet;
the purified animal tendon membrane is any one of purified buffalo tendon membrane, purified cattle tendon membrane, purified yak tendon membrane and purified goat tendon membrane;
the purified animal tendon particles are any one of purified buffalo tendon particles, purified cattle tendon particles, purified yak tendon particles and purified goat tendon particles.
The invention provides a bioactive collagen peptide finished product prepared by the preparation method of the bioactive collagen peptide.
Based on the technical scheme, the embodiment of the invention can at least produce the following technical effects:
(1) the preparation method of the bioactive collagen peptide and the bioactive collagen peptide provided by the invention take any one or more of purified animal dermal skin sheets, purified animal tendon membrane sheets and purified animal tendon particles as raw materials, and the raw materials have the advantages of rich sources, low price, easy obtainment and excellent quality. The bioactive collagen peptide prepared by the technology of the invention is prepared by two steps. Firstly, raw materials are subjected to a series of physical, chemical and biological processing methods such as weighing, shearing and/or crushing, softening, fluffing, homogenizing, dialysis, freeze drying and the like to prepare a high-level collagen aggregate; and secondly, performing controllable hydrolysis based on directional enzyme digestion on the high-level collagen aggregate under a controllable condition, and then performing cooling, centrifugation, concentration, spray drying and other processing to obtain the bioactive collagen peptide. The bioactive collagen peptide prepared by the method not only thoroughly eliminates antigen substances, but also ensures that the bioactive collagen peptide has excellent biocompatibility and good bioactivity, and can induce cell growth and tissue regeneration; moreover, the process operation is simple and easy to implement, the process is easy to control, and the quality of the active biological collagen peptide can be ensured.
(2) The bioactive collagen peptide obtained by the preparation method of the bioactive collagen peptide and the bioactive collagen peptide can be widely applied to the fields of polypeptide drug research and development, clinical medical treatment, food health care, medical cosmetology, skin care and the like.
(3) The raw materials, key equipment and key materials used by the preparation method of the bioactive collagen peptide and the bioactive collagen peptide provided by the invention are all made in China, so that the problem of elbow dragging of a person is solved, and the neck clamping is not worried about.
Detailed Description
Firstly, raw material description:
the purified animal dermal skin sheets, purified animal tendon membrane sheets and purified animal tendon granules used in examples 1 to 5 were all produced by Chengdu Handing New Material science and technology Co.
II, preparation example:
example 1:
s1, taking any one or more of purified animal dermal skin sheets, purified animal tendon membrane sheets and purified animal tendon particles as raw materials, sequentially weighing, shearing and/or crushing, softening, controlling water, fluffing, adjusting pH, homogenizing, dialyzing, and freeze-drying to obtain fluffy and porous high-level collagen aggregates, wherein the specific preparation steps are as follows:
s11, weighing
Selecting a purified pig dermis sheet as a raw material, and weighing the selected raw material;
s12, shearing and/or crushing
Cutting the purified pig dermis into small pieces to obtain small pieces, wherein the small pieces are rectangular, the length of the small pieces is 2-3cm, and the width of the small pieces is 2-3 cm;
s13, softening, namely placing the small leather sheets obtained in the step S12 in a low-temperature rotary drum, adding normal saline for soaking, wherein the addition amount of the normal saline is 2 times of the total weight of the purified pig dermis leather sheets, and soaking in two stages: the first stage soaking is to stand and soak for 30min at the temperature of 3.0 ℃; the second stage soaking is carried out at 3.0 deg.C under intermittent stirring, wherein the intermittent stirring is carried out for 3 times while rotating for 5min every 55 min; the small leather sheets are fully softened;
s14, controlling water
Draining the waste liquid soaked in the low-temperature rotary drum;
s15, puffing
Adding 10mM Tris-NaCl buffer solution with the pH value of 7.4 into a low-temperature rotary drum for soaking, wherein the Tris-NaCl buffer solution is prepared by uniformly mixing 5.844 parts by weight of sodium chloride, 0.6057 parts by weight of Tris (hydroxymethyl) aminomethane and 500 parts by weight of water for injection, the adding amount of the Tris-NaCl buffer solution is 15 times of the total weight of the purified pig dermis skin sheet, and soaking in two stages: the first stage soaking is to stand and soak at 3.0 deg.C for 110 min; the second stage of soaking is soaking at the temperature of 4 ℃ in an intermittent stirring state, wherein the intermittent stirring is performed for 4 times by rotating for 5min every 55min until the small leather sheets are semitransparent and have swelling feeling (slight swelling feeling), and fine particle suspension can be obviously observed; then removing clear liquid;
s16, adjusting pH, adding 0.45M acetic acid solution into the low-temperature rotary drum, wherein the adding amount is 2 times of the total weight of the purified pig dermis leather sheet, the pH is adjusted to be 2.5, and the liquid ratio is 1: 48; then standing and soaking for 1.5h until the small leather sheets are transparent;
s17 homogenate
Adding 0.45M acetic acid solution into the low-temperature rotary drum, wherein the addition amount of the acetic acid solution is 2 times of the total weight of the purified pig dermis and skin sheet, and then uniformly stirring; adding NaOH solution into the low-temperature rotary drum to adjust the pH value to 6.5; adding NaCl with the final concentration of 1.4M, and salting out for 22h until white flocculent separation appears; centrifuging at 3 deg.C and 8000r/min for 30min, collecting precipitate, and removing supernatant; eluting the precipitate with 0.45M acetic acid solution, and stirring at 3 deg.C to obtain high-level collagen aggregate dispersion;
s18, dialysis
Pouring the dispersion of high-level collagen aggregates into dialysis bag with dialysis molecular weight of 3k, 0.03mol/L and 0.01mol/LNa 2 HPO 4 Dialyzing the solution for 2d, dialyzing the solution for 2d by using distilled water, and changing the dialysate frequently;
s19, freeze drying
And (3) uniformly transferring the dialyzed high-level collagen aggregate into a glass dish, freezing and drying to obtain a fluffy and porous high-level collagen aggregate, and sealing, drying and storing for later use.
S2, sequentially carrying out ultrasonic treatment, enzymolysis, cooling, centrifugation, membrane concentration, spray drying, packaging and sterilization on the high-level collagen aggregate obtained in the step S1 to obtain a finished product of the bioactive collagen peptide, wherein the specific preparation steps are as follows:
s21, carrying out ultrasonic treatment, namely placing the high-level collagen aggregate prepared in the step S1 in an ultrasonic rotary drum, and adding distilled water into the ultrasonic rotary drum, wherein the adding amount of the distilled water is 2 times of the weight of the high-level collagen aggregate; starting the ultrasonic rotary drum, and rotating for 20 min; then, heating to 36 ℃ and keeping the temperature for 8 min;
s22, performing enzymolysis, namely adding 1mol/L NaOH or HCl solution into the ultrasonic rotary drum, and adjusting the pH value of the solution to 5.5; then, adding a complex enzyme preparation, wherein the weight ratio of the added amount of the complex enzyme preparation to the high-level collagen aggregate is 6: 100; then starting the ultrasonic rotary drum to rotate for 300 min; heating to 95 deg.C, maintaining the temperature for 5min to inactivate enzyme, and terminating hydrolysis reaction to obtain bioactive collagen peptide solution A;
the preparation method of the compound enzyme preparation comprises the following raw materials in parts by weight: 55 parts of papain, 25 parts of trypsin, 10 parts of alkaline protease, 5 parts of alpha-amylase and 5 parts of nano titanium dioxide;
s23, cooling, namely rapidly cooling the bioactive collagen peptide liquid A to room temperature;
s24, centrifuging, namely placing the bioactive collagen peptide liquid A cooled to room temperature in a high-speed centrifuge, centrifuging for 10min at 3500r/min, and taking supernatant to obtain bioactive collagen peptide liquid B;
s25, membrane concentration, namely separating and concentrating the bioactive collagen peptide liquid B by adopting a nanofiltration membrane to obtain a bioactive collagen peptide liquid C;
s26, spray drying, namely spray drying the bioactive collagen peptide liquid C on a spray dryer under the conditions that: the inlet temperature is 103 ℃ and the outlet temperature is 55 ℃; obtaining bioactive collagen peptide powder;
s27, packaging, wherein the packaging is carried out in a hundred-grade workshop and comprises inner packaging and outer packaging; the inner package is divided into an inner layer and an outer layer, the inner layer is a plastic film, and the outer layer is a tin foil bag; the outer package is a hard paper bag; firstly, carrying out inner layer packaging on a powder packaging machine, and then carrying out outer layer packaging and outer packaging on a clean workbench; finally, boxing according to the specification requirement;
s28, sterilizing, namely sterilizing the packaged bioactive collagen peptide powder, and sterilizing by using gamma rays generated by 10.0KGy/h60Co to obtain a bioactive collagen peptide finished product.
Example 2:
s1, taking any one or more of purified animal dermal skin sheets, purified animal tendon membrane sheets and purified animal tendon particles as raw materials, sequentially weighing, shearing and/or crushing, softening, controlling water, fluffing, adjusting pH, homogenizing, dialyzing, and freeze-drying to obtain fluffy and porous high-level collagen aggregates, wherein the specific preparation steps are as follows:
s11, weighing
Selecting a purified buffalo tendon membrane as a raw material, and weighing the selected raw material;
s12, cutting or crushing, namely crushing the purified buffalo tendon membrane into particles with the particle size of 1-2 cm;
s13, softening, namely putting the particles obtained in the step S12 into a low-temperature rotary drum, and adding normal saline for soaking, wherein the addition amount of the normal saline is 2.5 times of the total weight of the purified buffalo tendon membrane; the first stage soaking is to stand and soak at 3.5 deg.C for 60 min; the second stage soaking is carried out at 3.5 deg.C under intermittent stirring, wherein the intermittent stirring is carried out for 4 times while rotating for 5min every 55 min;
s14, controlling water, and drying the waste liquid soaked in the low-temperature rotary drum;
s15, fluffy loosening, adding 10mM Tris-NaCl buffer solution with the pH value of 7.4 into the low-temperature rotary drum for soaking, wherein the Tris-NaCl buffer solution is prepared by uniformly mixing 5.844 parts by weight of sodium chloride, 0.6057 parts by weight of Tris-aminomethane and 500 parts by weight of water for injection, and the addition amount of the Tris-NaCl buffer solution is 20 times of the total weight of the purified buffalo tendon membrane; soaking in two stages, wherein the soaking in the first stage is carried out by standing and soaking for 115min at the temperature of 3.5 ℃; the second stage of soaking is soaking at the temperature of 3.5 ℃ in an intermittent stirring state, wherein the intermittent stirring is performed for 6 times by rotating for 5min every 55min until the particles are semitransparent and have swelling feeling (slight swelling feeling), and fine particle suspension can be obviously observed; then removing clear liquid;
s16, adjusting pH, adding 0.5M acetic acid solution into the low-temperature rotary drum, wherein the addition amount of the acetic acid solution is 2.5 times of the total weight of the purified buffalo tendon membrane, the pH is adjusted to be 2.5, and the liquid ratio is 1: 50; then standing and soaking for 2 hours until the particles are transparent;
s17, homogenizing, adding 0.5M acetic acid solution into the low-temperature rotary drum, wherein the addition amount of the acetic acid solution is 2.5 times of the total weight of the purified buffalo tendon membrane, and then uniformly stirring; adding NaOH solution into the low-temperature rotary drum to adjust the pH value to 7.0; adding NaCl with the final concentration of 1.45M, and salting out for 24h until white flocculent separation appears; centrifuging at 3.5 deg.C and 9000r/min for 30min, collecting precipitate, and removing supernatant; eluting the precipitate with 0.5M acetic acid solution, and stirring at 3.5 deg.C to obtain high-level collagen aggregate dispersion;
s18, dialyzing, pouring the dispersion liquid of the high-level collagen aggregates into a dialysis bag with a dialysis molecular weight of 2.5k, and adding 0.04mol/L Na and 0.02mol/L Na 2 HPO 4 Dialyzing the solution for 2d, and then dialyzing the solution for 2d by using distilled water;
and S19, freeze drying, namely uniformly transferring the dialyzed high-level collagen aggregate into a glass dish for freeze drying to obtain fluffy and porous high-level collagen aggregate, and sealing, drying and storing for later use.
S2, sequentially carrying out ultrasonic treatment, enzymolysis, cooling, centrifugation, membrane concentration, spray drying, packaging and sterilization on the high-level collagen aggregate obtained in the step S1 to obtain a finished product of the bioactive collagen peptide, wherein the specific preparation steps are as follows:
s21, carrying out ultrasonic treatment, namely putting the high-level collagen aggregate prepared in the step S1 into an ultrasonic rotary drum, and adding distilled water into the ultrasonic rotary drum, wherein the adding amount of the distilled water is 2.5 times of the weight of the high-level collagen aggregate; starting the ultrasonic rotary drum, and rotating for 30 min; then, heating to 36 ℃ and keeping the temperature for 5 min;
s22, performing enzymolysis, namely adding 1mol/L NaOH or HCl solution into the ultrasonic rotary drum, and adjusting the pH value of the solution to 6; then, adding a complex enzyme preparation, wherein the weight ratio of the added amount of the complex enzyme preparation to the high-level collagen aggregate is 9: 100; then starting the ultrasonic rotary drum to rotate for 500 min; heating to 100 deg.C, keeping the temperature for 6min to inactivate enzyme, and terminating hydrolysis reaction to obtain bioactive collagen peptide solution A;
the preparation of the complex enzyme preparation comprises the following raw materials in parts by weight: 55 parts of papain, 22 parts of trypsin, 9 parts of alkaline protease, 5 parts of alpha-amylase and 4.5 parts of nano titanium dioxide;
s23, cooling, namely rapidly cooling the bioactive collagen peptide liquid A to room temperature;
s24, centrifuging, namely placing the bioactive collagen peptide liquid A cooled to room temperature in a high-speed centrifuge, centrifuging for 15min at 4000r/min, and taking supernatant to obtain bioactive collagen peptide liquid B;
s25, membrane concentration, namely separating and concentrating the bioactive collagen peptide liquid B by adopting a nanofiltration membrane to obtain a bioactive collagen peptide liquid C;
s26, spray drying, namely spray drying the bioactive collagen peptide liquid C on a spray dryer under the conditions that: the inlet temperature is 104 ℃ and the outlet temperature is 58 ℃; obtaining bioactive collagen peptide powder;
s27, packaging, wherein the packaging is carried out in a hundred-grade workshop and comprises inner packaging and outer packaging; the inner package is divided into an inner layer and an outer layer, the inner layer is a plastic film, and the outer layer is a tin foil bag; the outer package is a hard paper bag; firstly, carrying out inner layer packaging on a powder packaging machine, and then carrying out outer layer packaging and outer packaging on a clean workbench; finally, boxing is carried out according to the specification requirement;
s28, sterilizing, namely sterilizing the packaged bioactive collagen peptide powder, and sterilizing by using gamma rays generated by 15.0KGy/h60Co to obtain a bioactive collagen peptide finished product.
Example 3:
s1, taking any one or more of purified animal dermal skin sheets, purified animal tendon membrane sheets and purified animal tendon particles as raw materials, sequentially weighing, shearing and/or crushing, softening, controlling water, fluffing, adjusting pH, homogenizing, dialyzing, and freeze-drying to obtain fluffy and porous high-level collagen aggregates, wherein the specific preparation steps are as follows:
s11, weighing
Selecting purified beef tendon particles as raw materials, and weighing the selected raw materials;
s12, cutting or crushing, namely crushing the purified beef tendon particles into particles with the particle size of 0.5-0.8 cm;
s13, softening, namely putting the particles obtained in the step S12 into a low-temperature rotary drum, and adding normal saline for soaking, wherein the addition amount of the normal saline is 3 times of the total weight of the purified beef tendon particles; the soaking is carried out in two stages: the first stage of soaking is to stand and soak for 90min at the temperature of 4 ℃; the second stage of soaking is soaking at 4 deg.C under intermittent stirring, wherein the intermittent stirring is performed by rotating for 5min every 55min, and repeating for 5 times;
s14, controlling water, and drying the waste liquid soaked in the low-temperature rotary drum;
s15, fluffy bulking, and soaking in 10mM Tris-NaCl buffer solution with pH of 7.4 added into a low-temperature rotary drum, wherein the Tris-NaCl buffer solution is prepared by uniformly mixing 5.844 parts by weight of sodium chloride, 0.6057 parts by weight of Tris hydroxymethyl aminomethane and 500 parts by weight of water for injection, and the addition amount of the Tris-NaCl buffer solution is 25 times of the total weight of the purified beef tendon particles; soaking in two stages, wherein the soaking in the first stage is carried out by standing and soaking at 4 ℃ for 120 min; the second stage of soaking is soaking at 4 ℃ in an intermittent stirring state, wherein the intermittent stirring is performed for 8 times by rotating for 5min every 55min until the particles are semitransparent and swell (slightly), and fine particle suspension can be obviously observed; then removing clear liquid;
s16, adjusting pH, adding 0.5M acetic acid solution into the low-temperature rotary drum, wherein the adding amount is 3 times of the total weight of the purified beef tendon particles, the pH is adjusted to be 2, and the liquid ratio is 1: 50; then standing and soaking for 2 hours until the particles are transparent;
s17, homogenizing, adding 0.5M acetic acid solution into the low-temperature rotary drum, wherein the addition amount of the acetic acid solution is 3 times of the total weight of the purified beef tendon particles, and then uniformly stirring; adding NaOH solution into the low-temperature rotary drum to adjust the pH value to 7.0; adding NaCl with the final concentration of 1.5M, and salting out for 24h until white flocculent separation appears; centrifuging at 4.5 deg.C and 10000r/min for 30min, collecting precipitate, and removing supernatant; eluting the precipitate with 0.5M acetic acid solution, and stirring at 4.5 deg.C to obtain high-level collagen aggregate dispersion;
s18, dialyzing, pouring the dispersion liquid of the high-level collagen aggregates into a dialysis bag with a dialysis molecular weight of 2.0k, and adding 0.04mol/L and 0.02mol/L Na 2 HPO 4 Dialyzing the solution for 2d, and then dialyzing the solution for 2d by using distilled water;
and S19, freeze drying, namely uniformly transferring the dialyzed high-level collagen aggregate into a glass dish for freeze drying to obtain fluffy and porous high-level collagen aggregate, and sealing, drying and storing for later use.
S2, sequentially carrying out ultrasonic treatment, enzymolysis, cooling, centrifugation, membrane concentration, spray drying, packaging and sterilization on the high-level collagen aggregate obtained in the step S1 to obtain a finished product of the bioactive collagen peptide, wherein the specific preparation steps are as follows:
s21, carrying out ultrasonic treatment, namely placing the high-level collagen aggregate prepared in the step S1 in an ultrasonic rotary drum, and adding distilled water into the ultrasonic rotary drum, wherein the adding amount of the distilled water is 3 times of the weight of the high-level collagen aggregate; starting the ultrasonic rotary drum, and rotating for 40 min; then, heating to 45 ℃ and keeping the temperature for 6 min;
s22, performing enzymolysis, namely adding 1mol/L NaOH or HCl solution into the ultrasonic rotary drum, and adjusting the pH value of the solution to 8.5; then, adding a complex enzyme preparation, wherein the weight ratio of the added amount of the complex enzyme preparation to the high-level collagen aggregate is 12: 100; then starting the ultrasonic rotary drum to rotate for 1200 min; heating to 95 ℃, preserving the heat for 10min to inactivate the enzyme, and stopping the hydrolysis reaction to obtain a bioactive collagen peptide solution A;
the preparation method of the compound enzyme preparation comprises the following raw materials in parts by weight: 50 parts of papain, 30 parts of trypsin, 8 parts of alkaline protease, 6 parts of alpha-amylase and 4 parts of nano titanium dioxide;
s23, cooling, namely rapidly cooling the bioactive collagen peptide liquid A to room temperature;
s24, centrifuging, namely placing the bioactive collagen peptide liquid A cooled to room temperature in a high-speed centrifuge, centrifuging for 15min at 4000r/min, and taking supernatant to obtain bioactive collagen peptide liquid B;
s25, membrane concentration, namely separating and concentrating the bioactive collagen peptide liquid B by adopting a nanofiltration membrane to obtain a bioactive collagen peptide liquid C;
s26, spray drying, namely spray drying the bioactive collagen peptide liquid C on a spray dryer under the conditions that: the inlet temperature is 105 ℃ and the outlet temperature is 62 ℃; obtaining bioactive collagen peptide powder;
s27, packaging, wherein the packaging is carried out in a hundred-grade workshop and comprises inner packaging and outer packaging; the inner package is divided into an inner layer and an outer layer, the inner layer is a plastic film, and the outer layer is a tin foil bag; the outer package is a hard paper bag; firstly, carrying out inner layer packaging on a powder packaging machine, and then carrying out outer layer packaging and outer packaging on a clean workbench; finally, boxing according to the specification requirement;
s28, sterilizing, namely sterilizing the packaged bioactive collagen peptide powder, and sterilizing by using gamma rays generated by 20.0KGy/h60Co to obtain a bioactive collagen peptide finished product.
Example 4:
s1, taking any one or more of purified animal dermal skin sheets, purified animal tendon membrane sheets and purified animal tendon particles as raw materials, sequentially weighing, shearing and/or crushing, softening, controlling water, fluffing, adjusting pH, homogenizing, dialyzing, and freeze-drying to obtain fluffy and porous high-level collagen aggregates, wherein the specific preparation steps are as follows:
s11, weighing and selecting a purified fetal bovine dermal skin sheet and a purified goat dermal skin sheet as raw materials, and weighing the selected raw materials;
s12, cutting or crushing, namely cutting the purified fetal bovine dermal skin sheet and the purified goat dermal skin sheet into small pieces to obtain small pieces of skin sheets, wherein the small pieces of skin sheets are rectangular, the length of the small pieces of skin sheets is 2-3cm, and the width of the small pieces of skin sheets is 2-3 cm;
s13, softening, namely placing the small leather sheets obtained in the step S12 in a low-temperature rotary drum, and adding normal saline for soaking, wherein the addition amount of the normal saline is 3 times of the total weight of the purified fetal bovine dermal leather sheets and the purified goat dermal leather sheets; the first stage soaking is to stand and soak for 75min at the temperature of 4.5 ℃; the second stage of soaking is soaking at 4.5 deg.C under intermittent stirring, wherein the intermittent stirring is performed by rotating for 5-10min every 50-55min, and repeating for 3-5 times;
s14, controlling water, and drying the waste liquid soaked in the low-temperature rotary drum;
s15, fluffy loosening, namely adding 10mM Tris-NaCl buffer solution with the pH value of 7.4 into a low-temperature rotary drum for soaking, wherein the Tris-NaCl buffer solution is prepared by uniformly mixing 5.844 parts by weight of sodium chloride, 0.6057 parts by weight of Tris (hydroxymethyl) aminomethane and 500 parts by weight of water for injection, and the adding amount of the Tris-NaCl buffer solution is 20 times of the total weight of the purified fetal bovine dermal skin sheet and the purified goat dermal skin sheet; soaking in two stages, wherein the soaking in the first stage is carried out by standing and soaking at 4 ℃ for 120 min; the second stage of soaking is soaking at the temperature of 5 ℃ in an intermittent stirring state, wherein the intermittent stirring is performed for 5 times by rotating for 5min every 55min until the small leather sheets are semitransparent and have swelling feeling (slight swelling feeling), and fine particle suspension can be obviously observed; then removing clear liquid;
s16, adjusting the pH, adding 0.55M acetic acid solution into the low-temperature rotary drum, wherein the adding amount of the acetic acid solution is 2.5 times of the total weight of the purified fetal bovine dermal skin sheet and the purified goat dermal skin sheet, the pH is adjusted to 2.5, and the liquid ratio is 1: 50; then standing and soaking for 2.5h until the small leather sheets are transparent;
s17, homogenizing, adding 0.55M acetic acid solution into the low-temperature rotary drum, wherein the adding amount is 3 times of the total weight of the purified fetal bovine dermal skin sheet and the purified goat dermal skin sheet, and then uniformly stirring; adding NaOH solution into the low-temperature rotary drum to adjust the pH value to 7.0; adding NaCl with the final concentration of 1.5M, and salting out for 25h until white flocculent separation appears; centrifuging at 4.5 deg.C and 11000r/min for 33min, collecting precipitate, and removing supernatant; eluting the precipitate with 0.55M acetic acid solution, and stirring at 4.5 deg.C to obtain high-level collagen aggregate dispersion;
s18, dialyzing, pouring the dispersion liquid of the high-level collagen aggregates into a dialysis bag with a dialysis molecular weight of 3k, and adding 0.04mol/L Na and 0.02mol/L Na 2 HPO 4 Dialyzing the solution for 2d, and then dialyzing the solution for 2d by using distilled water;
s19, freeze drying, namely uniformly transferring the dialyzed high-level collagen aggregate into a glass dish for freeze drying to obtain fluffy and porous high-level collagen aggregate, and sealing, drying and storing for later use.
S2, sequentially carrying out ultrasonic treatment, enzymolysis, cooling, centrifugation, membrane concentration, spray drying, packaging and sterilization on the high-level collagen aggregate obtained in the step S1 to obtain a finished product of the bioactive collagen peptide, wherein the specific preparation steps are as follows:
s21, carrying out ultrasonic treatment, namely putting the high-level collagen aggregate prepared in the step S1 into an ultrasonic rotary drum, and adding distilled water into the ultrasonic rotary drum, wherein the adding amount of the distilled water is 2.5 times of the weight of the high-level collagen aggregate; starting the ultrasonic rotary drum, and rotating for 30 min; then, heating to 48 ℃ and keeping the temperature for 7 min;
s22, performing enzymolysis, namely adding 1mol/L NaOH or HCl solution into the ultrasonic rotary drum, and adjusting the pH value of the solution to 8.0; then, adding a complex enzyme preparation, wherein the weight ratio of the added amount of the complex enzyme preparation to the high-level collagen aggregate is 9: 100; then starting the ultrasonic rotary drum to rotate for 11000 min; heating to 98 ℃, keeping the temperature for 9min to inactivate enzyme, and stopping hydrolysis reaction to obtain bioactive collagen peptide solution A;
the preparation method of the compound enzyme preparation comprises the following raw materials in parts by weight: 60 parts of papain, 30 parts of trypsin, 12 parts of alkaline protease, 6 parts of alpha-amylase and 6 parts of nano titanium dioxide;
s23, cooling, namely rapidly cooling the bioactive collagen peptide liquid A to room temperature;
s24, centrifuging, namely placing the bioactive collagen peptide liquid A cooled to room temperature in a high-speed centrifuge, centrifuging for 18min at 4000r/min, and taking supernatant to obtain bioactive collagen peptide liquid B;
s25, membrane concentration, namely separating and concentrating the bioactive collagen peptide liquid B by adopting a nanofiltration membrane to obtain a bioactive collagen peptide liquid C;
and S26, spray drying, namely spray drying the bioactive collagen peptide liquid C on a spray dryer. The conditions of the spray drying were: the inlet temperature is 106 ℃, and the outlet temperature is 66 ℃, so as to obtain the bioactive collagen peptide powder;
s27, packaging, wherein the packaging is carried out in a hundred-grade workshop and comprises inner packaging and outer packaging; the inner package is divided into an inner layer and an outer layer, the inner layer is a plastic film, and the outer layer is a tin foil bag; the outer package is a hard paper bag; firstly, carrying out inner layer packaging on a powder packaging machine, and then carrying out outer layer packaging and outer packaging on a clean workbench; finally, boxing according to the specification requirement;
s28, sterilizing, namely sterilizing the packaged bioactive collagen peptide powder, and sterilizing by using gamma rays generated at a dose of 25KGy/h60Co to obtain a finished product of the bioactive collagen peptide.
Example 5:
s1, taking any one or more of purified animal dermal skin sheets, purified animal tendon membrane sheets and purified animal tendon particles as raw materials, sequentially weighing, shearing and/or crushing, softening, controlling water, fluffing, adjusting pH, homogenizing, dialyzing, and freeze-drying to obtain fluffy and porous high-level collagen aggregates, wherein the specific preparation steps are as follows:
s11, weighing
Selecting purified beef tendon membrane, purified yak tendon membrane and purified goat tendon particles as raw materials, and weighing the selected raw materials;
s12, cutting or crushing, namely crushing the purified beef tendon membrane, the purified yak tendon membrane and the purified goat tendon particles to obtain particles;
s13, softening, namely putting the particles obtained in the step S12 into a low-temperature rotary drum, and adding normal saline for soaking, wherein the addition amount of the normal saline is 2.5 times of the total weight of the purified beef tendon membrane, the purified yak tendon membrane and the purified goat tendon particles; the first stage soaking is to stand and soak for 90min at the temperature of 5.0 ℃; the second stage soaking is at 5.0 deg.C under intermittent stirring, wherein the intermittent stirring is performed by rotating for 5-10min every 50-55min, and repeating for 3-5 times;
s14, controlling water, and drying the waste liquid soaked in the low-temperature rotary drum;
s15, fluffy bulking, and adding 10mM Tris-NaCl buffer solution with the pH value of 7.4 into a low-temperature rotary drum, wherein the Tris-NaCl buffer solution is prepared by uniformly mixing 5.844 parts by weight of sodium chloride, 0.6057 parts by weight of Tris hydroxymethyl aminomethane and 500 parts by weight of water for injection, and the adding amount of the Tris-NaCl buffer solution is 25 times of the total weight of the purified cattle tendon membrane, the purified yak tendon membrane and the purified goat tendon particles; soaking in two stages, wherein the soaking in the first stage is carried out by standing and soaking at 4 ℃ for 120 min; the second stage of soaking is soaking at the temperature of 5 ℃ in an intermittent stirring state, wherein the intermittent stirring is performed for 3-5 times by rotating for 5-10min every 50-55min until small skin pieces/particles are semitransparent and swell, and fine particle suspension can be obviously observed; then removing clear liquid;
s16, adjusting the pH, adding 0.5M acetic acid solution into the low-temperature rotary drum, wherein the adding amount of the acetic acid solution is 3 times of the total weight of the purified beef tendon membrane, the purified yak tendon membrane and the purified goat tendon particles, the pH is adjusted to be 2.5, and the liquid ratio is 1: 52; then standing and soaking for 2.5h until the particles are transparent;
s17, homogenizing, adding 0.5M acetic acid solution into the low-temperature rotary drum, wherein the addition amount of the acetic acid solution is 3 times of the total weight of the purified beef tendon membrane, the purified yak tendon membrane and the purified goat tendon particles, and then uniformly stirring; adding NaOH solution into the low-temperature rotary drum to adjust the pH value to 7.5; adding NaCl with the final concentration of 1.5M, and salting out for 26h until white flocculent separation appears; centrifuging at 5 deg.C and 12000r/min for 35min, collecting precipitate, and removing supernatant; eluting the precipitate with 0.5M acetic acid solution, and stirring at 5 deg.C to obtain high-level collagen aggregate dispersion;
s18, dialyzing, namely pouring the dispersion liquid of the high-level collagen aggregates into a dialysis bag with a dialysis molecular weight of 1.0k, and adding 0.04mol/L Na and 0.02mol/L Na 2 HPO 4 Dialyzing the solution for 2d, and then dialyzing the solution for 2d by using distilled water;
and S19, freeze drying, namely uniformly transferring the dialyzed high-level collagen aggregate into a glass dish for freeze drying to obtain fluffy and porous high-level collagen aggregate, and sealing, drying and storing for later use.
S2, sequentially carrying out ultrasonic treatment, enzymolysis, cooling, centrifugation, membrane concentration, spray drying, packaging and sterilization on the high-level collagen aggregate obtained in the step S1 to obtain a finished product of the bioactive collagen peptide, wherein the specific preparation steps are as follows:
s21, carrying out ultrasonic treatment, namely placing the high-level collagen aggregate prepared in the step S1 in an ultrasonic rotary drum, and adding distilled water into the ultrasonic rotary drum, wherein the adding amount of the distilled water is 3 times of the weight of the high-level collagen aggregate; starting the ultrasonic rotary drum, and rotating for 40 min; then, heating to 52 ℃ and keeping the temperature for 8 min;
s22, performing enzymolysis, namely adding 1mol/L NaOH or HCl solution into the ultrasonic rotary drum, and adjusting the pH value of the solution to 8.5; then, adding a complex enzyme preparation, wherein the weight ratio of the addition amount of the complex enzyme preparation to the high-level collagen aggregate is 12: 100; then starting the ultrasonic rotary drum to rotate for 1200 min; heating to 100 deg.C, maintaining the temperature for 10min to inactivate enzyme, and terminating hydrolysis reaction to obtain bioactive collagen peptide solution A;
the preparation method of the compound enzyme preparation comprises the following raw materials in parts by weight: 55 parts of papain, 25 parts of trypsin, 10 parts of alkaline protease, 5 parts of alpha-amylase and 5 parts of nano titanium dioxide;
s23, cooling, namely rapidly cooling the bioactive collagen peptide liquid A to room temperature;
s24, centrifuging, namely placing the bioactive collagen peptide liquid A cooled to room temperature in a high-speed centrifuge, centrifuging for 20min at a speed of 4500r/min, and taking supernatant to obtain bioactive collagen peptide liquid B;
s25, membrane concentration, namely separating and concentrating the bioactive collagen peptide liquid B by adopting a nanofiltration membrane to obtain a bioactive collagen peptide liquid C;
s26, spray drying, namely, carrying out spray drying on the bioactive collagen peptide solution C on a spray dryer to obtain bioactive collagen peptide powder;
s27, packaging, wherein the packaging is carried out in a hundred-grade workshop and comprises inner packaging and outer packaging; the inner package is divided into an inner layer and an outer layer, the inner layer is a plastic film, and the outer layer is a tin foil bag; the outer package is a hard paper bag; firstly, carrying out inner layer packaging on a powder packaging machine, and then carrying out outer layer packaging and outer packaging on a clean workbench; finally, boxing according to the specification requirement;
s28, sterilizing, namely sterilizing the packaged bioactive collagen peptide powder, and sterilizing by using gamma rays generated by 30.0KGy/h60Co to obtain a bioactive collagen peptide finished product.
Third, Experimental example
1. Key properties of the bioactive collagen peptides prepared in examples 1 to 5 were examined
(1) The test standards or test methods are shown in table 1.
TABLE 1 determination of key properties of bioactive collagen peptides
(2) The results of the measurements are shown in Table 2.
TABLE 2 results of testing of Key Properties
As can be seen from table 2, the bioactive peptides prepared by the method of the present invention have good biocompatibility and high purity, and the active collagen peptides obtained in examples 1 to 5 of the present invention have no antigenic material from the viewpoint of in vitro cytotoxicity and oral acute toxicity; in addition, the in vitro cytotoxicity is 0-1 grade, the highest grade of the medical material is met, the requirements of the implant material are met, and the clinical application does not generate anaphylactic reaction and rejection reaction; according to the requirements of target products, the target bioactive collagen peptide meeting the requirements can be produced by selecting appropriate raw materials and adopting appropriate process conditions.
Claims (10)
1. A preparation method of bioactive collagen peptide is characterized by comprising the following steps:
s1, taking any one or more of a purified animal dermal skin sheet, a purified animal tendon membrane sheet and a purified animal tendon particle as a raw material, sequentially weighing, shearing and/or crushing, softening, controlling water, fluffing, adjusting pH, homogenizing, dialyzing, and freeze-drying to obtain a fluffy and porous high-level collagen aggregate;
and S2, sequentially carrying out ultrasonic treatment, enzymolysis, cooling, centrifugation, membrane concentration, spray drying, packaging and sterilization on the high-level collagen aggregate obtained in the step S1 to obtain a finished product of the bioactive collagen peptide.
2. The method for preparing bioactive collagen peptide according to claim 1, wherein in step S1, the specific steps for preparing the high-grade collagen aggregate are:
s11, weighing
Weighing the selected raw materials;
s12, cutting or crushing
When the selected raw materials comprise purified animal dermis sheets, cutting the purified animal dermis sheets into small pieces to obtain small pieces of sheets; when the selected raw materials comprise purified animal tendon diaphragms or purified animal tendon particles, the purified animal tendon diaphragms or the purified animal tendon particles are crushed to obtain finer particles;
s13 softening
Placing the small leather sheets or particles obtained in the step S12 into a low-temperature rotary drum, and adding normal saline for soaking, wherein the soaking is divided into two stages: the first stage soaking is to stand and soak at 3.0-5.0 deg.C for 30-90 min; the second stage of soaking is to soak for 180 min at the temperature of 3.0-5.0 ℃ in an intermittent stirring state;
s14, controlling water
Draining the waste liquid soaked in the low-temperature rotary drum;
s15, puffing
Adding Tris-NaCl buffer solution into the low-temperature rotary drum for soaking, wherein the soaking is divided into two stages: the first stage of soaking is to stand and soak for 110-; the second stage of soaking is to soak for 180-480min at the temperature of 3-5 ℃ under the intermittent stirring state; when the selected raw materials comprise purified animal dermis leather sheets, the soaking is finished until small leather sheets are semitransparent and have swelling feeling; when the selected raw materials comprise purified animal tendon membranes or purified animal tendon particles, the soaking is finished until fine particles can be obviously observed to suspend; then removing clear liquid;
s16, adjusting pH
Adding an acetic acid solution into the low-temperature rotary drum, adjusting the pH to 2-2.5, and mixing the solution in a ratio of 1: 48-52; then standing and soaking for 1.5-2.5h until the small leather sheets and/or the particles are transparent;
s17 refining
Adding acetic acid solution into the low-temperature rotary drum, and uniformly stirring; then adding NaOH solution into the low-temperature rotary drum to adjust the pH value to 6.5-7.5; adding NaCl with final concentration of 1.40-1.60M, and salting out for 22-26h until white flocculent separation appears; centrifuging at 3-5 deg.C and 8000-; eluting the precipitate with acetic acid solution, and stirring at 3-5 deg.C to obtain high-level collagen aggregate dispersion;
s18, dialysis
Pouring the dispersion of high-level collagen aggregate into dialysis bag with dialysis molecular weight of 1.0-4.0k, 0.03-0.05mol/L and 0.01-0.03mol/L Na 2 HPO 4 Dialyzing the solution for 1.5-2.5 days, and dialyzing the solution for 1.5-2.5 days by using distilled water;
s19, freeze drying
And (3) uniformly transferring the dialyzed high-level collagen aggregate into a glass vessel for freeze drying to obtain fluffy and porous high-level collagen aggregate, and sealing, drying and storing for later use.
3. The method of claim 2, wherein in step S12, the parchment piece has a rectangular shape with a length of 2-3cm and a width of 2-3 cm;
in the step S13, the intermittent stirring is performed by rotating for 5-10min every 50-55min and repeating for 3-5 times; the addition amount of the normal saline is 2-3 times of the total weight of the raw materials;
in the step S15, the intermittent stirring is performed by rotating for 5-10min every 50-55min and repeating for 3-6 times;
in step S15, the amount of Tris-NaCl buffer solution is 10mM, and the addition amount is 12-25 times of the total weight of the raw materials.
4. The method for preparing bioactive collagen peptide according to claim 2, wherein in S16, the acetic acid solution is 0.45-0.55M, and the addition amount is 2-3 times of the total weight of the raw materials;
in the step 17, the acetic acid solution is 0.45M-0.55M, and the addition amount of the acetic acid solution is 2-3 times of the total weight of the raw materials.
5. The method for preparing a bioactive collagen peptide according to claim 1, wherein in the step S2, the specific steps of preparing the finished bioactive collagen peptide are as follows:
s21 ultrasonic treatment
Placing the high-level collagen aggregate prepared in the step S1 in an ultrasonic rotary drum, adding distilled water into the ultrasonic rotary drum, starting the ultrasonic rotary drum, and rotating for 20-40 min; then heating to 36-52 ℃ and preserving heat for 4-8 min;
s22, enzymolysis
Adding NaOH or HCl solution into the ultrasonic rotary drum, and adjusting the pH value of the solution to 5.5-8.5; then, adding a complex enzyme preparation, and starting the ultrasonic rotary drum to rotate for 300-1200 min; heating to 95-100 deg.C, maintaining the temperature for 5-10min to inactivate enzyme, and terminating hydrolysis reaction to obtain bioactive collagen peptide solution A;
s23, cooling
Rapidly cooling the bioactive collagen peptide solution A to room temperature;
s24 centrifugation
Placing the bioactive collagen peptide liquid A cooled to room temperature in a high-speed centrifuge, centrifuging for 10-20min at 3500-4500r/min, and taking the supernatant to obtain bioactive collagen peptide liquid B;
s25, membrane concentration
Separating and concentrating the bioactive collagen peptide liquid B by adopting a nanofiltration membrane to obtain bioactive collagen peptide liquid C;
s26, spray drying
Carrying out spray drying on the bioactive collagen peptide liquid C on a spray dryer to obtain bioactive collagen peptide powder;
s27, packaging
The packaging is carried out in a hundred-grade workshop and is divided into inner packaging and outer packaging; the inner package is divided into an inner layer and an outer layer, the inner layer is a plastic film, and the outer layer is a tin foil bag; the outer package is a hard paper bag; firstly, carrying out inner layer packaging on a powder packaging machine, and then carrying out outer layer packaging and outer packaging on a clean workbench; finally, boxing according to the specification requirement;
s28, sterilization
Sterilizing the packaged bioactive collagen peptide powder, and sterilizing by using gamma rays generated by 10.0-30.0KGy/h60Co to obtain the bioactive collagen peptide finished product.
6. The method for producing bioactive collagen peptide according to claim 5, wherein in step S21, the amount of distilled water added is 2 to 3 times the weight of the higher-grade collagen aggregate;
in step S26, the spray drying conditions are: the inlet temperature is 103-107 ℃ and the outlet temperature is 55-65 ℃.
7. The method for preparing bioactive collagen peptide according to claim 5, wherein in step S22, the NaOH solution or the HCl solution is 0.9-1.1 mol/L; the weight ratio of the added amount of the complex enzyme preparation to the high-level collagen aggregate is 6-12: 100; the preparation of the compound enzyme preparation comprises the following raw materials in parts by weight: 50-60 parts of papain, 20-30 parts of trypsin, 8-12 parts of alkaline protease, 4-6 parts of alpha-amylase and 4-6 parts of nano titanium dioxide.
8. The method for preparing bioactive collagen peptide according to claim 7, wherein in the step S22, the preparation of the complex enzyme preparation comprises the following raw materials in parts by weight: 55 parts of papain, 25 parts of trypsin, 10 parts of alkaline protease, 5 parts of alpha-amylase and 5 parts of nano titanium dioxide.
9. The method of producing a bioactive collagen peptide according to any one of claims 1 to 8, wherein in step S1, the purified animal dermal skin is any one of a purified pig dermal skin, a purified fetal bovine dermal skin, and a purified goat dermal skin;
the purified animal tendon membrane is any one of purified buffalo tendon membrane, purified cattle tendon membrane, purified yak tendon membrane and purified goat tendon membrane;
the purified animal tendon particles are any one of purified buffalo tendon particles, purified cattle tendon particles, purified yak tendon particles and purified goat tendon particles.
10. A finished bioactive collagen peptide product prepared by the method of any one of claims 1 to 9.
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