CN115069314B - Microfluidic chip - Google Patents
Microfluidic chip Download PDFInfo
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- CN115069314B CN115069314B CN202110268570.9A CN202110268570A CN115069314B CN 115069314 B CN115069314 B CN 115069314B CN 202110268570 A CN202110268570 A CN 202110268570A CN 115069314 B CN115069314 B CN 115069314B
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 98
- 239000012530 fluid Substances 0.000 claims abstract description 62
- 238000002347 injection Methods 0.000 claims abstract description 53
- 239000007924 injection Substances 0.000 claims abstract description 53
- 238000004806 packaging method and process Methods 0.000 claims description 10
- 238000004891 communication Methods 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 108020004414 DNA Proteins 0.000 description 13
- 230000003321 amplification Effects 0.000 description 13
- 238000003199 nucleic acid amplification method Methods 0.000 description 13
- 238000000034 method Methods 0.000 description 10
- 238000000137 annealing Methods 0.000 description 7
- 238000004925 denaturation Methods 0.000 description 7
- 230000036425 denaturation Effects 0.000 description 7
- 102000053602 DNA Human genes 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000005086 pumping Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000007599 discharging Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000005842 biochemical reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000003437 trachea Anatomy 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The embodiment of the application provides a microfluidic chip, which comprises: the fluid reaction unit comprises a sample inlet end and a sample outlet end; the sample injection pipeline is communicated with the sample injection end of the fluid reaction unit; the sample outlet pipeline is communicated with the sample outlet end of the fluid reaction unit; the first air pipe is communicated with the sample injection pipeline; and the second air pipe is communicated with the sample outlet pipeline. The microfluidic chip can greatly shorten the length of the channel in the microfluidic reaction unit, miniaturize the microfluidic chip, reduce the production cost of the microfluidic chip and improve the portability of the microfluidic chip.
Description
Technical Field
The invention relates to the technical field of molecular biology, in particular to a microfluidic chip.
Background
The microfluidic chip is a new technology which is developed in the fields of medicine, biology, life science and the like at present, and is an emerging technology for integrating basic operations such as sample preparation, biochemical reaction, liquid separation, detection analysis and the like related in chemistry and biology onto a chip with the size of a few square centimeters so as to complete different biochemical reactions, and is a sample mixing, separating, product detection and analysis technology.
In a specific working process of the microfluidic chip, a sample enters the fluid reaction unit through a sample inlet end of the microfluidic chip, and is discharged out of the microfluidic chip through a sample outlet end of the microfluidic chip after the reaction of the fluid reaction unit, so that in order to ensure the reaction efficiency and the full progress of the reaction, a long single channel or multiple channels are required to be arranged in the microfluidic chip in the prior art, which leads to the large volume of the microfluidic chip, high production cost and poor portability of the microfluidic chip in the prior art.
Disclosure of Invention
The present invention aims to solve at least one of the technical problems existing in the prior art or related art.
In view of this, there is provided a microfluidic chip according to an embodiment of the present application, including: the fluid reaction unit comprises a sample inlet end and a sample outlet end; the sample injection pipeline is communicated with the sample injection end of the fluid reaction unit; the sample outlet pipeline is communicated with the sample outlet end of the fluid reaction unit; the first air pipe is communicated with the sample injection pipeline; and the second air pipe is communicated with the sample outlet pipeline.
In a first possible implementation of an embodiment of the present application, the microfluidic chip further comprises: the sample feeding pipeline, the sample discharging pipeline, the first air pipe and the second air pipe are provided with at least one control valve.
In a second possible implementation manner of the embodiment of the present application, one of the plurality of control valves is a first control valve, and the first control valve is disposed on the first air pipe; one of the plurality of control valves is a second control valve, and the second control valve is arranged on a second air pipe; one of the plurality of control valves is a third control valve which is arranged on the sample injection pipeline; one of the plurality of control valves is a fourth control valve which is arranged on the sample outlet pipeline; one of the plurality of control valves is a fifth control valve, and the fifth control valve is arranged at the communication position of the sample outlet pipeline and the second air pipe.
In a third possible implementation of an embodiment of the present application, the microfluidic chip further includes: the packaging assembly, the fluid reaction unit, the sample injection pipeline, the sample outlet pipeline, the first air pipe, the second air pipe and the plurality of control valves are arranged in the packaging assembly.
In a fourth possible implementation manner of the embodiment of the present application, the package assembly includes: a first plate body; the second plate body, fluid reaction unit, advance the appearance pipeline, go out appearance pipeline, first trachea and second trachea setting on the second plate body, first plate body is connected in the second plate body.
In a fifth possible implementation of an embodiment of the present application, each control valve includes: the first valve body is arranged on the first plate body; the second valve body is arranged on the second plate body, and the first valve body is used for being connected with the second valve body; the flexible film is arranged between the first plate body and the second plate body; the control air hole is formed in the first plate body, and when the first valve body is ventilated through the control air hole, the valve core of the first valve body stretches out to push the flexible film to seal the second valve body.
In a sixth possible implementation manner of the embodiment of the present application, the microfluidic chip further includes: the sample injection hole assembly is arranged on the first plate body and/or the second plate body and is communicated with the sample injection pipeline; the sample outlet hole assembly is arranged on the first plate body and/or the second plate body and is communicated with the sample outlet pipeline; the first air hole assembly is arranged on the first plate body and/or the second plate body and is communicated with the first air pipe; the second air hole component is arranged on the first plate body and/or the second plate body and is communicated with the second air pipe.
In a seventh possible implementation manner of the embodiment of the present application, the sample injection hole assembly includes: the first sample injection hole is arranged on the first plate body; the second sample injection hole is arranged on the second plate body, and the first sample injection hole is connected with the second sample injection hole under the condition that the second plate body is connected with the first plate body; the sample well assembly includes: the first sample outlet hole is arranged on the first plate body; the second sample outlet is arranged on the second plate body, and the first sample outlet is connected with the second sample outlet under the condition that the second plate body is connected with the first plate body; the first air hole assembly includes: the first air hole is arranged on the first plate body; the second air hole is formed in the second plate body, and the first air hole is connected with the second air hole under the condition that the second plate body is connected with the first plate body; the second air hole assembly includes: the third air hole is arranged on the first plate body; and the fourth air hole is arranged on the second plate body, and is connected with the third air hole under the condition that the second plate body is connected with the first plate body.
In an eighth possible implementation manner of the embodiment of the present application, the microfluidic chip further includes: the positioning hole is arranged on one of the first plate body and the second plate body; and the positioning column is arranged on the other one of the first plate body and the second plate body and is used for being inserted into the positioning hole.
In a ninth possible implementation manner of the embodiment of the present application, the fluid reaction unit includes: a plurality of isothermal reaction zones, the temperature between the plurality of isothermal reaction zones being different.
Compared with the prior art, the application at least comprises the following beneficial effects: according to the microfluidic chip provided by the embodiment of the application, through the arrangement of the fluid reaction unit, the sample inlet pipeline, the sample outlet pipeline, the first air pipe and the second air pipe, in the working process, a sample is conveyed to the microfluidic reaction unit through the sample inlet pipeline, then the first air pipe is inflated for the first time, and the first-time inflated gas can push the sample in the fluid reaction unit to move to the sample outlet end through the sample inlet end of the fluid reaction unit, so that the sample can perform a one-time reaction in the fluid reaction chip. And then, the second air pipe is inflated for the second time, and the gas inflated for the second time can push the sample in the fluid reaction unit to move to one side of the sample inlet end through the sample outlet end. Then, the first air pipe is inflated for the third time, and the gas inflated for the third time can push the sample in the fluid reaction unit to move to the sample outlet end through the sample inlet end of the fluid reaction unit, so that the sample can react again in the fluid reaction chip, and the sample can be pushed to move in the microfluidic reaction unit in a reciprocating manner through the first air pipe and the second air pipe, so that the sample can move to the sample outlet end through the sample inlet end of the fluid reaction unit in a reciprocating manner, the sample can react for multiple times, the length of a channel in the microfluidic reaction unit can be greatly shortened, the microfluidic chip can be miniaturized, the production cost of the microfluidic chip is reduced, and the portability of the microfluidic chip is improved.
Drawings
The foregoing and/or additional aspects and advantages of the invention will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:
FIG. 1 is a schematic diagram of a microfluidic chip according to an embodiment of the present application;
Fig. 2 is a schematic structural diagram of a first board body of a microfluidic chip according to an embodiment of the present application;
fig. 3 is a schematic structural diagram of a second board body of a microfluidic chip according to an embodiment of the present application;
fig. 4 is a schematic structural diagram of a second plate of a microfluidic chip according to another embodiment of the present application.
The correspondence between the reference numerals and the component names in fig. 1 to 4 is:
A fluid reaction unit, a2 sample injection pipeline, a 3 sample outlet pipeline, a 4 first air pipe, a 5 second air pipe, a 6 first control valve, a 7 second control valve, a 8 third control valve, a 9 fourth control valve, a 10 fifth control valve, a 11 packaging assembly, a 12 first plate body, a 13 second plate body, a 14 first valve body, a 15 second valve body, a 16 sample injection hole assembly, a 17 sample outlet hole assembly, a 18 first air hole assembly, a 19 second air hole assembly, a 20 positioning hole and a 21 positioning column;
1601 first sample injection hole, 1602 second sample injection hole;
1701 a first sample outlet, 1702 a second sample outlet;
1801 first air holes, 1802 second air holes;
1901 third air holes, 1902 fourth air holes.
Detailed Description
So that the manner in which the above recited objects, features and advantages of the present application can be understood in detail, a more particular description of the application, briefly summarized below, may be had by reference to the appended drawings. It should be noted that, without conflict, the embodiments of the present application and features in the embodiments may be combined with each other.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those described herein, and therefore the scope of the present invention is not limited to the specific embodiments disclosed below.
As shown in fig. 1, one embodiment of the present invention provides a microfluidic chip comprising: the fluid reaction unit 1, the fluid reaction unit 1 includes a sample inlet end and a sample outlet end; the sample injection pipeline 2 is communicated with the sample injection end of the fluid reaction unit 1; the sample outlet pipe 3 is communicated with the sample outlet end of the fluid reaction unit 1; the first gas pipe 4 is communicated with the sample injection pipeline 2; and the second air pipe 5 is communicated with the outlet pipe 3.
According to the microfluidic chip provided by the embodiment of the application, through the arrangement of the fluid reaction unit 1, the sample injection pipeline 2, the sample outlet pipeline 3, the first gas pipe 4 and the second gas pipe 5, in the working process, a sample is conveyed to the microfluidic reaction unit 1 through the sample injection pipeline 2, then the first gas pipe 4 is inflated for the first time, and the gas filled for the first time can push the sample in the fluid reaction unit 1 to move to the sample outlet end through the sample injection end of the fluid reaction unit 1, so that the sample can perform a reaction in the fluid reaction chip. Then, the second air pipe 5 is inflated for the second time, and the gas inflated for the second time can push the sample in the fluid reaction unit 1 to move to the sample inlet end side through the sample outlet end. Then, the first air pipe 4 is inflated for the third time, and the gas inflated for the third time can push the sample in the fluid reaction unit 1 to move to the sample outlet end through the sample inlet end of the fluid reaction unit 1, so that the sample can react again in the fluid reaction chip, and the sample can be pushed to move in the micro-fluid reaction unit 1 in a reciprocating manner through the first air pipe 4 and the second air pipe 5, so that the sample can move to the sample outlet end through the sample inlet end of the fluid reaction unit 1 in a reciprocating manner, the sample can react for a plurality of times, the length of a channel in the micro-fluid reaction unit 1 can be greatly shortened, the production cost of the micro-fluid chip can be reduced, and the portability of the micro-fluid chip can be improved.
In some examples, where the sample is sufficiently reacted within the microfluidic reaction unit 1, the sample may be inflated through the first gas tube 4 to move the sample out of the microfluidic chip via the outlet tube 3.
In some examples, the sample in the fluid reaction unit 1 may be moved to the sample-introducing end side via the sample-introducing end by sucking air via the first air tube 4 as well, and the sample in the fluid reaction unit 1 may be moved to the sample-introducing end side via the sample-introducing end by sucking air via the second air tube 5 as well.
In some examples, during pushing of the sample through the sample inlet end to the sample outlet end, the sample may be inflated through the first gas tube 4 while being aspirated through the second gas tube 5; in the process of pushing the sample to move to the sample inlet end through the sample outlet end, the sample can be pumped through the first air pipe 4 and simultaneously inflated through the second air pipe 5. The inventors have found that a stable movement of the sample within the microfluidic reaction unit 1 is facilitated by the gas filling being synchronized with the extraction.
It will be appreciated that during inflation or deflation via the first and second gas lines 4, 5, the sample inlet and outlet lines 2, 3 should be closed to prevent sample overflow; for the same reason, the first gas tube 4 and the second gas tube 5 should be closed when a sample is injected into the microfluidic reaction unit 1 via the sample introduction tube 2.
In some examples, a plurality of constant temperature regions are provided within the microfluidic reaction unit 1, and the same or different constant temperature may be provided for the plurality of constant temperature regions to facilitate the reaction of the sample.
Taking the amplification of deoxyribonucleic acid (DeoxyriboNucleic Acid, DNA) by the fluid reaction unit 1 as an example, a high temperature denaturation temperature region, a low temperature annealing temperature region and a pairing amplification temperature region which are sequentially communicated may be included in the microfluidic reaction unit 1. After the sample is input into the fluid reaction unit 1, the sample is driven to sequentially pass through a high-temperature denaturation temperature zone, a low-temperature annealing temperature zone and a pairing amplification temperature zone by air inflation of a first pipeline or air suction of a second pipeline. Firstly, the sample is in a high-temperature denaturation temperature zone, and DNA in the sample is melted into a single strand; and then the sample is annealed at a low temperature in a low temperature annealing temperature zone, and finally the DNA which is melted into a single strand in a pairing amplification temperature zone can be paired with a template in a base complementary mode through a primer in the sample, so that DNA double-strand synthesis is realized to realize selective in vitro semi-reserved replication of double-strand DNA fragments, and the primary amplification of the DNA can be realized. And then pumping air through a first pipeline or pumping air through a second pipeline, so that the sample subjected to primary amplification returns to the sample inlet end through the sample outlet end of the fluid reaction unit 1, and the sample is driven to pass through the high-temperature denaturation temperature region, the low-temperature annealing temperature region and the pairing amplification temperature region again through the pumping air of the first pipeline or the pumping air of the second pipeline, so that the secondary amplification can be performed. By such circulation, millions of DNA fragments can be generated after 25 to 30 cycles, thereby rapidly achieving the purpose of amplifying a large amount of template DNA. The reciprocating movement of the sample in the fluid reaction unit 1 is controlled by the air flow in the whole amplification process, so that the length of the flow path of the sample in the fluid reaction unit 1 is relatively increased, the length of the channel in the fluid reaction unit 1 can be greatly shortened, the micro-fluidic chip can be miniaturized, the production cost of the micro-fluidic chip is reduced, and the portability of the micro-fluidic chip is improved.
Wherein the constant temperature of the high temperature denaturation temperature region is 90-95 ℃, the constant temperature of the low temperature annealing temperature region is 55-60 ℃, and the constant temperature of the pairing amplification temperature region is 70-72 ℃.
As shown in fig. 4, in some examples, the microfluidic chip further comprises: the control valves are arranged on at least one of the sample inlet pipeline 2, the sample outlet pipeline 3, the first air pipe 4 and the second air pipe 5.
As shown in fig. 4, the microfluidic chip further includes a plurality of control valves, where the control valves are disposed on one or more of the sample inlet pipe 2, the sample outlet pipe 3, the first gas pipe 4 and the second gas pipe 5, and the input and output of the sample are conveniently controlled by the setting of the plurality of control valves, so that the gas is conveniently inflated and pumped, the sample is prevented from overflowing, the sample loss is avoided, and the safe and smooth reaction is ensured.
As shown in fig. 4, in some examples, one of the plurality of control valves is a first control valve 6, the first control valve 6 being disposed on the first gas pipe 4; one of the plurality of control valves is a second control valve 7, and the second control valve 7 is arranged on the second air pipe 5; one of the plurality of control valves is a third control valve 8, and the third control valve 8 is arranged on the sample injection pipeline 2; one of the plurality of control valves is a fourth control valve 9, and the fourth control valve 9 is arranged on the sample outlet pipeline 3; one of the plurality of control valves is a fifth control valve 10, and the fifth control valve 10 is arranged at the communication position of the sample outlet pipeline 3 and the second air pipe 5.
As shown in fig. 4, a first control valve 6, a second control valve 7, a third control valve 8, a fourth control valve 9, and a fifth control valve 10 are included.
In the process of inputting the sample into the fluid reaction unit 1 through the sample introduction pipeline 2, in order to ensure the stability of the pressure in the microfluidic chip and avoid the overflow of the sample through the first air pipe 4 and the second air pipe 5, the first control valve 6 and the second control valve 7 can be controlled to be closed, and the third control valve 8, the fourth control valve 9 and the fifth control valve 10 can be controlled to be opened.
In order to avoid overflow of the sample through the sample introduction pipe 2 or the sample outlet pipe 3 during the process of driving the sample to reciprocate in the microfluidic reaction unit 1 by the air flow, the first control valve 6, the second control valve 7 and the fifth control valve 10 can be controlled to be opened, and the third control valve 8 and the fourth control valve 9 can be controlled to be closed.
In order to accurately control the removal of the sample through the sample outlet channel 3 after the completion of the sample reaction, the first control valve 6, the fourth control valve 9, the fifth control valve 10 may be controlled to be opened and the second control valve 7 and the third control valve 8 may be controlled to be closed in the process of discharging the microfluidic chip through the sample outlet channel 3.
As shown in fig. 4, the fifth control valve 10 is arranged to directly close the outlet pipe 3 and the second air pipe 5, which is beneficial to control of the microfluidic chip in emergency, and can directly disconnect the outlet of the sample.
In some examples, the fifth control valve 10 may also be a two-way valve to selectively conduct one of the sample outlet channel 3 and the second air tube 5, so that the control of the microfluidic chip is more accurate, for example, during the process of inputting the sample into the microfluidic reaction unit 1, the microfluidic reaction unit 1 is selected to be conducted with the sample outlet channel 3 by the fifth control valve 10; during the reciprocating movement of the sample in the microfluidic reaction unit 1, the microfluidic reaction unit 1 is selected to be communicated with the second air tube 5 by the fifth control valve 10; in case the sample has completed the reaction and the fluid reaction unit 1 is removed, the microfluidic reaction unit 1 is selected to be in communication with the outlet conduit 3 by means of the fifth control valve 10.
As shown in fig. 1, in some examples, the microfluidic chip further comprises: the packaging assembly 11, the fluid reaction unit 1, the sample introduction pipeline 2, the sample outlet pipeline 3, the first air pipe 4, the second air pipe 5 and the plurality of control valves are arranged in the packaging assembly 11.
As shown in fig. 1, through the arrangement of the packaging component 11, the fluid reaction unit 1, the sample injection pipeline 2, the sample outlet pipeline 3, the first air pipe 4, the second air pipe 5 and the plurality of control valves can be packaged, so that the mechanical performance of the microfluidic chip can be improved, the devices of the microfluidic chip can be protected, the service life of the microfluidic chip can be prolonged, and the microfluidic chip is more portable.
As shown in fig. 2 and 3, in some examples, the package assembly 11 includes: a first plate 12; the second plate 13, the fluid reaction unit 1, the sample introduction pipeline 2, the sample outlet pipeline 3, the first gas pipe 4 and the second gas pipe 5 are arranged on the second plate 13, and the first plate 12 is connected to the second plate 13.
As shown in fig. 2 and 3, the packaging assembly 11 includes a first plate 12 and a second plate 13, and the first plate 12 and the second plate 13 clamp the packaged fluid reaction unit 1, the sample feeding pipe 2, the sample discharging pipe 3, the first air pipe 4 and the second air pipe 5 are disposed on the second plate 13, so as to facilitate the processing and preparation of the microfluidic chip, simplify the preparation process, and further reduce the production cost.
As shown in fig. 2 and 3, in some examples, each control valve includes: a first valve body 14 provided on the first plate body 12; a second valve body 15 disposed on the second plate 13, the first valve body 14 being adapted to be connected to the second valve body 15; a flexible film disposed between the first plate body 12 and the second plate body 13; the control air hole is formed in the first plate body 12, and when the first valve body 14 is ventilated through the control air hole, the valve core of the first valve body 14 stretches out to push the flexible film to seal the second valve body 15.
As shown in fig. 2 and 3, in this embodiment, each control valve includes a first valve body 14, a second valve body 15, a flexible film and a control air hole, and in the working process, the valve core of the first valve body 14 can be controlled to extend by ventilation into the control air hole, after the valve core extends, the flexible film can be pushed to move to the second valve body 15 side, so that the closing of the control valve can be realized, and the control valve is opened without ventilation through the control air hole. The arrangement can control the opening and closing of the control valve in a pneumatic mode through the outside of the packaging assembly 11, and is beneficial to intelligent control of the micro-fluid chip.
As shown in fig. 4, in some examples, the microfluidic chip further comprises: the sample injection hole assembly 16 is arranged on the first plate body 12 and/or the second plate body 13 and is communicated with the sample injection pipeline 2; the sample outlet hole assembly 17 is arranged on the first plate body 12 and/or the second plate body 13 and is communicated with the sample outlet pipe 3; the first air hole assembly 18 is arranged on the first plate body 12 and/or the second plate body 13 and is communicated with the first air pipe 4; the second air hole assembly 19 is arranged on the first plate body 12 and/or the second plate body 13 and is communicated with the second air pipe 5.
As shown in fig. 4, the microfluidic chip further includes: sample inlet assembly 16, sample outlet assembly 17, first air vent assembly 18, second air vent assembly 19. Through the arrangement of the sample inlet hole assembly 16 and the sample outlet hole assembly 17, the sample is conveniently input into the sample inlet pipeline 2, and meanwhile, the sample is conveniently removed through the sample outlet pipeline 3, and the sample removed through the microfluidic chip is conveniently accepted. The first air hole assembly 18 and the second air hole assembly 19 are arranged, so that air injection and extraction are facilitated.
As shown in fig. 2 and 3, in some examples, the feed port assembly 16 includes: a first sample inlet 1601 disposed on the first plate 12; the second sample injection hole 1602 is disposed on the second plate 13, and when the second plate 13 is connected to the first plate 12: the first sample injection hole 1601 is connected with the second sample injection hole 1602; the sample well assembly 17 includes: a first sample outlet 1701 provided in the first plate 12; a second sample outlet 1702 provided in the second plate 13, the first sample outlet 1701 being connected to the second sample outlet 1702 when the second plate 13 is connected to the first plate 12; the first air hole assembly 18 includes: a first air hole 1081 provided in the first plate body 12; a second air hole 1802 provided in the second plate 13, the first air hole 1801 being connected to the second air hole 1802 when the second plate 13 is connected to the first plate 12; the second air hole assembly 19 includes: a third air hole 1901 provided in the first plate 12; the fourth air hole 1902 is provided in the second plate 13, and when the second plate 13 is connected to the first plate, the fourth air hole 1902 is connected to the third air hole 1901.
As shown in fig. 2 and 3, the sample injection hole assembly 16 is of a split design, and includes a first sample injection hole 1601 formed on the first plate 12 and a second sample injection hole 1602 formed on the second plate 13, so that the two sides of the microfluidic chip can be injected with samples, and the microfluidic chip is more convenient to use. It will be appreciated that when a sample is injected via one of the first injection hole 1601 and the second injection hole 1602, the other of the first injection hole 1601 and the second injection hole 1602 should be plugged.
As shown in fig. 2 and 3, the sample outlet assembly 17 is of a split design, and includes a first sample outlet 1701 disposed on the first plate 12 and a second sample outlet 1702 disposed on the second plate 13, so that both sides of the microfluidic chip can be removed from the sample, and the microfluidic chip is more convenient to use. It will be appreciated that when a sample is injected through one of the first and second wells 1701, 1702, the other of the first and second wells 1701, 1702 should be plugged.
As shown in fig. 2 and 3, the first air hole assembly 18 is of a split design, and includes a first air hole 1801 disposed on the first plate 12 and a second air hole 1802 disposed on the second plate 13, so that both sides of the microfluidic chip can be inflated or deflated for the first air tube 4, and the microfluidic chip is more convenient to use. It will be appreciated that when a sample is injected through one of the first and second air holes 1801, 1802, the other of the first and second air holes 1801, 1802 should be plugged.
As shown in fig. 2 and 3, the second air hole assembly 19 is of a split design, and includes a third air hole 1901 formed in the first plate 12 and a fourth air hole 1902 formed in the second plate 13, so that both sides of the microfluidic chip can be inflated or deflated for the second air pipe 5, and the microfluidic chip is more convenient to use. It will be appreciated that when a sample is injected through one of the third and fourth air holes 1901, 1902, the other of the third and fourth sample outlet holes 1901, 1902 should be plugged.
As shown in fig. 2 and 3, in some examples, the microfluidic chip further comprises: a positioning hole 20 provided on one of the first plate body 12 and the second plate body 13; the positioning column 21 is arranged on the other of the first plate body 12 and the second plate body 13, and the positioning column 21 is used for being inserted into the positioning hole 20.
As shown in fig. 2 and 3, the positioning connection of the first plate 12 and the second plate 13 is facilitated by the positioning holes 20 and the positioning columns 21, so that the packaging of the microfluidic chip is more convenient.
In some examples, the fluid reaction unit 1 comprises: a plurality of isothermal reaction zones, the temperature between the plurality of isothermal reaction zones being different.
By arranging a plurality of constant temperature reaction areas, different reaction temperatures can be provided for the samples in the fluid reaction unit 1, and the reaction is facilitated.
Taking the amplification of deoxyribonucleic acid (DeoxyriboNucleic Acid, DNA) by the fluid reaction unit 1 as an example, a high temperature denaturation temperature region, a low temperature annealing temperature region and a pairing amplification temperature region which are sequentially communicated may be included in the microfluidic reaction unit 1. Wherein the constant temperature of the high temperature denaturation temperature region is 90-95 ℃, the constant temperature of the low temperature annealing temperature region is 55-60 ℃, and the constant temperature of the pairing amplification temperature region is 70-72 ℃.
As shown in fig. 3 and fig. 4, in some examples, the microfluidic reaction unit 1 includes a reaction channel, where a plurality of isothermal reactors are formed on the reaction channel, and the reaction channel is arranged on the second plate 13 in a folded manner, so that the volume of the microfluidic chip can be further reduced.
In the description of the present invention, the term "plurality" means two or more, unless explicitly defined otherwise, the orientation or positional relationship indicated by the terms "upper", "lower", etc. are based on the orientation or positional relationship shown in the drawings, merely for convenience of description of the present invention and to simplify the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as limiting the present invention; the terms "coupled," "mounted," "secured," and the like are to be construed broadly, and may be fixedly coupled, detachably coupled, or integrally connected, for example; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
In the description of the present invention, the terms "one embodiment," "some embodiments," "particular embodiments," and the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In the present invention, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A microfluidic chip, comprising:
The fluid reaction unit comprises a sample inlet end and a sample outlet end;
The sample injection pipeline is communicated with the sample injection end of the fluid reaction unit;
the sample outlet pipeline is communicated with the sample outlet end of the fluid reaction unit;
the first air pipe is communicated with the sample injection pipeline;
the second air pipe is communicated with the sample outlet pipeline;
the sample inlet pipeline, the sample outlet pipeline, the first air pipe and the second air pipe are provided with the control valves;
the fluid reaction unit, the sample injection pipeline, the sample outlet pipeline, the first air pipe, the second air pipe and the plurality of control valves are arranged in the packaging assembly;
the package assembly includes:
A first plate body;
The fluid reaction unit, the sample injection pipeline, the sample outlet pipeline, the first air pipe and the second air pipe are arranged on the second plate body, and the first plate body is connected with the second plate body;
each of the control valves includes:
the first valve body is arranged on the first plate body;
The second valve body is arranged on the second plate body, and the first valve body is used for being connected with the second valve body;
a flexible film disposed between the first plate and the second plate;
the control air hole is formed in the first plate body, and when the first valve body is ventilated through the control air hole, the valve core of the first valve body stretches out to push the flexible film to seal the second valve body.
2. The microfluidic chip of claim 1, wherein the microfluidic chip comprises,
One of the control valves is a first control valve, and the first control valve is arranged on a first air pipe;
One of the control valves is a second control valve, and the second control valve is arranged on a second air pipe;
One of the control valves is a third control valve, and the third control valve is arranged on the sample injection pipeline;
One of the control valves is a fourth control valve which is arranged on the sample outlet pipeline;
one of the control valves is a fifth control valve, and the fifth control valve is arranged at the communication position of the sample outlet pipeline and the second air pipe.
3. The microfluidic chip of claim 1, further comprising:
The sample injection hole assembly is arranged on the first plate body and/or the second plate body and is communicated with the sample injection pipeline;
The sample outlet hole assembly is arranged on the first plate body and/or the second plate body and is communicated with the sample outlet pipeline;
The first air hole assembly is arranged on the first plate body and/or the second plate body and is communicated with the first air pipe;
the second air hole component is arranged on the first plate body and/or the second plate body and is communicated with the second air pipe.
4. The microfluidic chip according to claim 3, wherein the microfluidic chip comprises,
The sample injection hole assembly comprises:
the first sample injection hole is arranged on the first plate body;
the second sample injection hole is arranged on the second plate body, and the first sample injection hole is connected with the second sample injection hole under the condition that the second plate body is connected with the first plate body;
the sample well assembly comprises:
The first sample outlet hole is arranged on the first plate body;
the second sample outlet is arranged on the second plate body, and the first sample outlet is connected with the second sample outlet under the condition that the second plate body is connected with the first plate body;
the first air hole assembly includes:
the first air hole is arranged on the first plate body;
The second air hole is formed in the second plate body, and the first air hole is connected with the second air hole under the condition that the second plate body is connected with the first plate body;
The second air hole assembly includes:
The third air hole is arranged on the first plate body;
And the fourth air hole is formed in the second plate body, and is connected with the third air hole under the condition that the second plate body is connected with the first plate body.
5. The microfluidic chip of claim 1, further comprising:
A positioning hole provided on one of the first plate body and the second plate body;
the positioning column is arranged on the other one of the first plate body and the second plate body and is used for being inserted into the positioning hole.
6. The microfluidic chip according to any one of claims 1 to 2, wherein said fluid reaction unit comprises:
A plurality of isothermal reaction zones, the temperature between the plurality of isothermal reaction zones being different.
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| CN202110268570.9A CN115069314B (en) | 2021-03-12 | 2021-03-12 | Microfluidic chip |
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| CN202110268570.9A CN115069314B (en) | 2021-03-12 | 2021-03-12 | Microfluidic chip |
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| CN101613660A (en) * | 2002-12-30 | 2009-12-30 | 加州大学评议会 | Detect and analyze the method and apparatus of pathogenic agent |
| CN103282782A (en) * | 2010-10-27 | 2013-09-04 | 株式会社百奥尼 | Automatic real-ime pcr system for the various analysis of biological smaple |
| CN105170206A (en) * | 2015-09-24 | 2015-12-23 | 基蛋生物科技股份有限公司 | Micro-fluidic chip achieving multi-index detection |
| CN106582905A (en) * | 2017-01-12 | 2017-04-26 | 上海小海龟科技有限公司 | Sample introduction system for microfluid chips |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004283083A (en) * | 2003-03-24 | 2004-10-14 | Hitachi Ltd | On-line chemical reaction unit and analysis system therefor |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101613660A (en) * | 2002-12-30 | 2009-12-30 | 加州大学评议会 | Detect and analyze the method and apparatus of pathogenic agent |
| CN103282782A (en) * | 2010-10-27 | 2013-09-04 | 株式会社百奥尼 | Automatic real-ime pcr system for the various analysis of biological smaple |
| CN105170206A (en) * | 2015-09-24 | 2015-12-23 | 基蛋生物科技股份有限公司 | Micro-fluidic chip achieving multi-index detection |
| CN106582905A (en) * | 2017-01-12 | 2017-04-26 | 上海小海龟科技有限公司 | Sample introduction system for microfluid chips |
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| CN115069314A (en) | 2022-09-20 |
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