CN115067275B - Three-dimensional compound bezoar cultivation method in vivo - Google Patents
Three-dimensional compound bezoar cultivation method in vivo Download PDFInfo
- Publication number
- CN115067275B CN115067275B CN202210870068.XA CN202210870068A CN115067275B CN 115067275 B CN115067275 B CN 115067275B CN 202210870068 A CN202210870068 A CN 202210870068A CN 115067275 B CN115067275 B CN 115067275B
- Authority
- CN
- China
- Prior art keywords
- bezoar
- bed
- bile
- feeding
- gallbladder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 206010004542 Bezoar Diseases 0.000 title claims abstract description 87
- 238000001727 in vivo Methods 0.000 title claims abstract description 15
- 150000001875 compounds Chemical class 0.000 title claims abstract description 12
- 238000012364 cultivation method Methods 0.000 title claims abstract description 10
- 210000000941 bile Anatomy 0.000 claims abstract description 60
- 241000894006 Bacteria Species 0.000 claims abstract description 17
- 239000003833 bile salt Substances 0.000 claims abstract description 17
- 239000000654 additive Substances 0.000 claims abstract description 15
- 239000002245 particle Substances 0.000 claims abstract description 10
- 210000000232 gallbladder Anatomy 0.000 claims description 62
- 241000283690 Bos taurus Species 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 21
- 238000001914 filtration Methods 0.000 claims description 13
- 238000009395 breeding Methods 0.000 claims description 12
- 230000001488 breeding effect Effects 0.000 claims description 11
- 230000028327 secretion Effects 0.000 claims description 10
- 241000588724 Escherichia coli Species 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 8
- 241000287828 Gallus gallus Species 0.000 claims description 7
- 230000000996 additive effect Effects 0.000 claims description 7
- 210000003746 feather Anatomy 0.000 claims description 7
- 239000002184 metal Substances 0.000 claims description 7
- 239000004677 Nylon Substances 0.000 claims description 6
- 201000001883 cholelithiasis Diseases 0.000 claims description 6
- 210000003205 muscle Anatomy 0.000 claims description 6
- 229920001778 nylon Polymers 0.000 claims description 6
- 244000025254 Cannabis sativa Species 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 4
- 206010061218 Inflammation Diseases 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 4
- 239000004459 forage Substances 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 230000004054 inflammatory process Effects 0.000 claims description 4
- 230000002045 lasting effect Effects 0.000 claims description 4
- 239000000178 monomer Substances 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 206010002091 Anaesthesia Diseases 0.000 claims description 3
- 208000002847 Surgical Wound Diseases 0.000 claims description 3
- 210000003489 abdominal muscle Anatomy 0.000 claims description 3
- 230000002745 absorbent Effects 0.000 claims description 3
- 239000002250 absorbent Substances 0.000 claims description 3
- 230000037005 anaesthesia Effects 0.000 claims description 3
- 238000007457 cholecystostomy Methods 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 230000008602 contraction Effects 0.000 claims description 3
- QTCANKDTWWSCMR-UHFFFAOYSA-N costic aldehyde Natural products C1CCC(=C)C2CC(C(=C)C=O)CCC21C QTCANKDTWWSCMR-UHFFFAOYSA-N 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 210000000936 intestine Anatomy 0.000 claims description 3
- ISTFUJWTQAMRGA-UHFFFAOYSA-N iso-beta-costal Natural products C1C(C(=C)C=O)CCC2(C)CCCC(C)=C21 ISTFUJWTQAMRGA-UHFFFAOYSA-N 0.000 claims description 3
- 210000004303 peritoneum Anatomy 0.000 claims description 3
- 230000035790 physiological processes and functions Effects 0.000 claims description 3
- 230000002980 postoperative effect Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000003307 slaughter Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 210000002784 stomach Anatomy 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims 1
- 208000001130 gallstones Diseases 0.000 description 13
- 230000036541 health Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 210000002919 epithelial cell Anatomy 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 2
- 206010010904 Convulsion Diseases 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 206010062717 Increased upper airway secretion Diseases 0.000 description 2
- 206010022998 Irritability Diseases 0.000 description 2
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 2
- 210000000013 bile duct Anatomy 0.000 description 2
- 229940093761 bile salts Drugs 0.000 description 2
- 230000036461 convulsion Effects 0.000 description 2
- 230000008021 deposition Effects 0.000 description 2
- 210000000981 epithelium Anatomy 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- 230000031864 metaphase Effects 0.000 description 2
- 208000026435 phlegm Diseases 0.000 description 2
- 239000006187 pill Substances 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 210000000813 small intestine Anatomy 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- BHQCQFFYRZLCQQ-UHFFFAOYSA-N (3alpha,5alpha,7alpha,12alpha)-3,7,12-trihydroxy-cholan-24-oic acid Natural products OC1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 BHQCQFFYRZLCQQ-UHFFFAOYSA-N 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 102000004452 Arginase Human genes 0.000 description 1
- 108700024123 Arginases Proteins 0.000 description 1
- 241001536324 Botryococcus Species 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- 239000004380 Cholic acid Substances 0.000 description 1
- 206010010071 Coma Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 230000001914 calming effect Effects 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 229960002471 cholic acid Drugs 0.000 description 1
- 235000019416 cholic acid Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 206010015037 epilepsy Diseases 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 208000021760 high fever Diseases 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000013777 protein digestion Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 206010042772 syncope Diseases 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 235000019871 vegetable fat Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/37—Digestive system
- A61K35/413—Gall bladder; Bile
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Physiology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Nutrition Science (AREA)
- Birds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a three-dimensional compound bezoar in vivo cultivation method, and relates to the field of bezoar in vivo cultivation. The in vivo cultivation method of the three-dimensional compound bezoar comprises the following steps of S1, implanting a bezoar bed, S2: implanting bezoar bacteria, S3, feeding, S4, adding additives for feeding; the three-dimensional compound bezoar cultivation method in vivo. Has the characteristic of better adhesiveness, and is easier to gather free bile salt particles in the bile.
Description
Technical Field
The invention relates to the technical field of in vivo cultivation of bezoar, in particular to a three-dimensional compound in vivo cultivation method of bezoar.
Background
Bezoar is a precious traditional Chinese medicinal material, has wide application range in disease treatment, high medicinal value, cool nature, bitter taste and sweet taste, has the functions of clearing heat, detoxifying, inducing resuscitation, eliminating phlegm, calming wind, arresting convulsion and the like, is suitable for symptoms such as high fever, coma, epilepsy, convulsion, apoplexy and phlegm syncope, is an important precious raw material for preparing AngongNiuhuang pills, purple snow Dan, liushen pills and the like, is calculus which is gradually formed in a gall bladder or a bile duct of a cow under pathological conditions, has the natural bezoar with the output from a special diseased cow body of less than 0.2 percent and is difficult to meet the requirements of a medicinal market since ancient times, and is always a very short-lived medicament.
However, the yield of the existing artificial cultivated bezoar is still very low due to immature technology, on one hand, the yield is far from the production requirement, on the other hand, the price is extremely high, so that a plurality of low-income patients cannot use the bezoar, at present, artificial bezoar has to be used in a plurality of medicinal materials, and the efficacy of the artificial bezoar is greatly reduced although the cost is reduced.
After the gall-bladder was implanted to traditional bezoar bed, because the adsorption rate is low, tiny bile salt that forms in the gall-bladder can not fine adsorb on the bezoar bed, and discharge external along with the bile to the small intestine along with excrement and urine, the white waste a lot of resources, some meeting of traditional bezoar bed block up the gall pipe mouth in addition, lead to bile secretion obstructed, direct influence acceptor cattle's health.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention discloses a three-dimensional compound bezoar cultivation method in vivo, which aims to solve the problems in the background technology.
(II) technical scheme
In order to realize the purpose, the invention is realized by the following technical scheme: the three-dimensional compound bezoar cultivation method in vivo comprises the following steps:
s1, implanting a bezoar bed, and selecting 6-month-old bred cows to perform conventional cholecystotomy;
s2: implanting calculus bovis bacteria into the gallbladder of cattle;
s3, feeding, wherein conventional forage grass is used in the breeding process;
the feeding principle of big and small adjustment is adopted, the total feeding amount is unchanged, but the feeding method of indefinite and indefinite amount and the feeding after 30 minutes of small-dose feeding are adopted, and the secretion of bile is induced to increase by utilizing the principle that food reflects to the stomach and intestine, so that more bezoar is formed;
s4, feeding by adding additives, wherein self-prepared additives are periodically added in the feeding process;
the additive is a natural plant additive prepared from herba Lysimachiae Christinae, stigma Maydis, herba Artemisiae Scopariae, etc., and can promote bile secretion, accelerate calculus bovis generation, and reduce the influence of cholelithiasis on normal physiological function of cattle;
s5, taking yellow, slaughtering after finishing breeding (about 30 months old) and marketing, taking out the gall bladder, rapidly absorbing excess gall from the gall by using materials such as absorbent paper and the like, drying in an oven at 50 ℃, cleaning out a bezoar bed and other impurities, and sealing and storing in a colored glass bottle.
Preferably, in S1, the specific operations are as follows:
feeding is forbidden in the first 24 hours, and water is forbidden in 8 hours;
performing surgical disinfection and anesthesia, wherein a surgical incision is formed between the reciprocal 2 and 3 ribs of the right body wall of a young cow, the lower edge of the longissimus dorsi and the midpoint of the upper edge of 11 costal arches and is 8 cm long, and the skin, the external oblique muscles, the internal oblique muscles and the peritoneum are surgically cut to find a gallbladder;
if the gall bladder is full, a part of the bile is extracted by the injector, a disposable full-layer incision about 1.5 cm is made in the gall bladder, the bezoar bed is placed, 1ml of diluted bacterium solution (containing more than 50 hundred million of viable bacteria) is dripped, full-layer spiral continuous suture is made on the first layer of the gall bladder, and vertical inward turning continuous suture of a pulp-muscle layer is made on the second layer of the gall bladder, so that the bile cannot leak;
suturing abdominal muscle and skin, performing postoperative care for 1 week, removing skin suture line after 8 days, and recovering conventional breeding.
Preferably, the bezoar bovis bacteria is an escherichia coli strain separated from the gall bladder of white feather broiler chicken, and can survive for a long time after being planted in the gall bladder of cattle after being adapted to the environment of the gall bladder of cattle through culture medium culture, and the generated inflammatory reaction is slight and lasting.
Preferably, the two monomers are overlapped to double the filtering area, so that the filtering of bile salt particles can be improved, and the filtered bile salt can be prevented from falling off again;
the nylon filter screen is additionally provided with the cylindrical net-shaped metal cover, so that the filtering area can be increased, the stability of the bezoar bed is increased, and the bezoar bed can be prevented from being extruded and deformed or even being extruded and flattened by the contraction of the gallbladder when the gallbladder is empty;
the combination of the inner and outer layers makes the density of the whole bed close to and slightly lower than that of bile, so that the bed can be suspended in the bile, and the bed is completely wrapped by the bezoar because the center of the bed is not filled with a solid body.
Preferably, at the initial stage of implanting the bezoar bed into the gall bladder, the bezoar bed can be distributed at the mouth of the gall bladder due to buoyancy and force generated by outflow of the bile, can play a role of a bile filter, and can adsorb fine gallstone particles dissociating in the bile into the net;
after adsorbing for a certain time, the bile will suspend in the bile along with the increase of the weight, thus realizing the collection and fixation of the initial gallstones, and not affecting the normal discharge of the bile, and greatly improving the adhesiveness of the gallstone particles.
The invention discloses a three-dimensional compound bezoar internal cultivating method, which has the following beneficial effects:
compared with the traditional bezoar bed, the three-dimensional compound bezoar in vivo cultivation method has the characteristics of better adhesiveness and easier aggregation of free bile salt particles in bile;
the adopted bezoar bacteria is an escherichia coli strain separated from the gall bladder of white feather broiler chicken by technicians of our company, can survive for a long time after being planted in the gall bladder of cattle after being adapted to the environment of the gall bladder of cattle through culture medium culture, and has slight and lasting inflammatory reaction and stronger yellow production capacity.
The cattle after artificial planting adopts a self-created feeding mode in the feeding process and combines natural plant Chinese herbal medicine additives capable of promoting bile secretion to increase the yield of the bezoar.
Drawings
FIG. 1 is a schematic view of a surgical site of the present invention;
FIG. 2 is a schematic view of the internal structure of the bezoar bed of the present invention;
FIG. 3 is a schematic view of the external metal cylinder of the bezoar bed of the present invention;
FIG. 4 is a schematic diagram of the cultivation of the strain of Botryococcus bovis of the present invention;
FIG. 5 is a graph showing the difference between the bezoar of the present invention and the conventional colonized bezoar (the national standard requires no less than 35% bilirubin in the bezoar and no less than 7% cholic acid).
Detailed Description
With the accompanying drawings of the specification and figures 1-4, the three-dimensional compound bezoar in vivo cultivation method comprises the following steps:
s1, implanting the cattle into a bezoar bed, and selecting 6-month-old bred cattle to perform conventional cholecystotomy;
s2: implanting bezoar bacteria into the gallbladder of the cattle;
s3, feeding, wherein conventional forage grass is used in the breeding process;
the feeding principle of big and small adjustment is adopted, the total feeding amount is unchanged, but the feeding method of indefinite and indefinite amount and the feeding after 30 minutes of small-dose feeding are adopted, and the secretion of bile is induced to increase by utilizing the principle that food reflects to the stomach and intestine, so that more bezoar is formed;
the cattle subjected to the artificial planting operation still uses conventional forage grass in the breeding process, adopts a conventional breeding method, and implements the principle of' big unchanged and small adjustment
(1) The total feeding amount is consistent with the conventional feeding method so as to ensure the growth speed of the bred cattle.
(2) Breaks through the conventional feeding mode with fixed time and fixed quantity, and adopts the unconventional feeding method regularly, so that the bile stored in the gallbladder can not be discharged to duodenum in time, and is beneficial to the deposition of gallstones. The methods include irregular hunger method, small amount of food calling, regulation of the addition ratio of vegetable oil and fat, and Chinese herbal medicine additive.
The calculus bovis product produced by the method has no obvious difference with the components of natural calculus bovis through the analysis and judgment of the medicine inspection in the market of great expense, and can be completely used for medicine production and processing.
S4, feeding by adding additives, wherein self-prepared additives are periodically added in the feeding process;
the additive is a natural plant additive prepared from herba Lysimachiae Christinae, stigma Maydis, herba Artemisiae Scopariae, etc., and can promote bile secretion, accelerate calculus bovis generation, and reduce the influence of cholelithiasis on normal physiological function of cattle;
s5, taking the yellow, slaughtering when finishing the breeding (about 30 months old) and selling on the market, taking out the gall bladder, quickly absorbing excess bile by using materials such as absorbent paper and the like, drying in an oven at 50 ℃, cleaning out a bezoar bed and other impurities, and sealing and storing in a colored glass bottle.
In the step S1, the specific operation is as follows:
feeding is forbidden in the first 24 hours, and water is forbidden in 8 hours;
performing surgical disinfection and anesthesia, wherein a surgical incision is formed between the reciprocal 2 and 3 ribs of the right body wall of a young cow, the lower edge of the longissimus dorsi and the midpoint of the upper edge of 11 costal arches and is 8 cm long, and the skin, the external oblique muscles, the internal oblique muscles and the peritoneum are surgically cut to find a gallbladder;
if the gall bladder is full, a part of the bile is extracted by the injector, a disposable full-layer incision about 1.5 cm is made in the gall bladder, the bezoar bed is placed, 1ml of diluted bacterium solution (containing more than 50 hundred million of viable bacteria) is dripped, full-layer spiral continuous suture is made on the first layer of the gall bladder, and vertical inward turning continuous suture of a pulp-muscle layer is made on the second layer of the gall bladder, so that the bile cannot leak;
suturing abdominal muscle and skin, performing postoperative care for 1 week, removing skin suture line after 8 days, and recovering conventional breeding.
Different from the traditional calculus bovis bed with a traditional structure, the light metal mesh-type cylindrical calculus bovis bed designed by our company is internally provided with a special cotton fiber mesh, so that the filterability of the calculus bovis granules and the loss rate of bile salt calculus are greatly improved, the production capacity of calculus bovis is remarkably improved, and the stimulation to the gallbladder is small.
The traditional bezoar bed has the advantages that firstly, the total adsorption area is small, the adsorption rate of bile salts is low, secondly, small bile salt blocks formed in the early stage of the traditional bezoar bed are easy to fall off and fall into bile, and are wasted along with the discharge of the bile, and moreover, because the density of the calculus bovis is higher than that of the bile after the calculus bovis is attached to the traditional bezoar bed, the calculus bovis can always sink to the bottom of a gall bladder, the bile at the bottom is low in fluidity, the bile amount passing through the bed body is small, the deposition of the bile salts is small, and the calculus bovis bed is in contact with the inner wall of the gall bladder for a long time to generate strong stimulation to epithelial cells, so that the secretion of the bile is inhibited, and the calculus bovis is not beneficial to the formation and the health of cattle.
The bezoar bacteria is an escherichia coli strain separated from the gall bladder of white feather broiler chicken, can survive for a long time after being planted in the gall bladder of cattle after being adapted to the environment of the gall bladder of cattle through culture of a culture medium, and generates slight and lasting inflammatory reaction.
The two monomers are overlapped to double the filtering area, so that the filtering of bile salt particles can be improved, and the filtered bile salt can be prevented from falling off again;
the nylon filter screen is additionally provided with the layer of cylindrical net-shaped metal cover, so that the filtering area can be increased, the stability of the bezoar bed is increased, and the bezoar bed can be prevented from being extruded and deformed or even being extruded and flattened when the gallbladder is empty;
the ectonexine structure combines together, the density that makes whole bed is close and slightly less than with the bile, can let it suspend in the bile, because its center can not be filled for the solid body, even bed body is wrapped up by the bezoar completely, the buoyancy that hollow structure produced also can let the bed body can suspend in the bile at the initial stage that the bezoar was cultivated, earlier stage, metaphase, later stage, only can suspend in the bile at the bottom of the gallbladder bag after gathering calculus bovis to special big weight with the bezoar bed, because almost can not direct contact to the gall-bladder inner wall, the irritability to gall-bladder epithelial cell and tissue reduces, collect the health that can more guarantee the acceptor ox when the gallstone.
At the initial stage of implanting the bezoar bed into the gall bladder, the bezoar bed can be distributed at the mouth of the gall bladder due to buoyancy and force generated by bile outflow, can play a role of a bile filter, and can adsorb fine gallstone particles dissociating in the bile into a net;
after adsorbing for a certain time, the bile will suspend in the bile along with the increase of the weight, thereby not only realizing the collection and fixation of the initial gallstones, but also not influencing the normal discharge of the bile, and greatly improving the adhesiveness of the gallstone particles
The introduction of the bezoar bacteria, the bezoar bacteria currently used in China are mainly escherichia coli separated in a gall bladder, and some strains such as O147, O8, O14 and the like use actinomycetes and bereksels, while a technical group separates the escherichia coli from white feather broiler bile through screening, and the escherichia coli is identified to belong to the O14 strain by microorganism experts of inner Mongolia agriculture university, because poultry lack arginase which is possessed by mammals, the final product after protein digestion is urate, and the white feather broiler is a poultry variety which is most likely to form calculus, so the escherichia coli strain is more suitable for environments with high bile salt and high urate, biochemical tests are carried out on the white feather broiler bile, and the escherichia coli which can grow in the gall bladder of a cow is screened out and inoculated into the bile, and the bezoar generation speed can be improved.
This patent has carried out technical innovation to old-fashioned bezoar bed, has all carried out big improvement from structure, material, and several improve the aspect and summarize as follows greatly:
(1) The original bezoar bed is changed into a net structure made of nylon materials, and the two monomers are overlapped, so that the filtering area is doubled, the filtering of bile salt particles can be improved, and the bile salt filtered can be reduced from falling off again.
(2) The nylon filter screen is additionally provided with the layer of cylindrical net-shaped metal cover, so that the filtering area can be increased, the stability of the bezoar bed is increased, and the bezoar bed can be prevented from being extruded and deformed or even being extruded and flattened by the contraction of the gallbladder when the gallbladder is empty.
(3) The ectonexine structure combines together, the density that makes whole bed is close and slightly less than with the bile, can let it suspend in the bile, because its center can not be filled for the solid body, even bed body is wrapped up by the bezoar completely, the buoyancy that hollow structure produced also can let the bed body can suspend in the bile at the initial stage that the bezoar was cultivated, earlier stage, metaphase, later stage, only can suspend in the bile at the bottom of the gallbladder bag after gathering calculus bovis to special big weight with the bezoar bed, because almost can not direct contact to the gall-bladder inner wall, the irritability to gall-bladder epithelial cell and tissue reduces, collect the health that can more guarantee the acceptor ox when the gallstone.
What this patent is by several technologies constitute jointly, and main patent technology is novel bezoar bed, and after traditional bezoar bed implanted the gall-bladder, because the adsorption efficiency is low, tiny bile salt that forms in the gall-bladder can not fine adsorb on the bezoar bed, and discharge along with the bile to the small intestine along with excrement and urine extravagant a lot of resources, some meeting of traditional bezoar bed block up the bile duct mouth in addition, lead to bile secretion to be obstructed, directly influence the health of acceptor ox.
This patent adopts light metal netted cylinder bezoar bed for the first time in this trade as peripheral frame, and the internal multilayer cotton fiber cobweb-shaped filtration that attaches.
Implant the gall-bladder initial stage, because buoyancy and the bile outflow form the strength, it can distribute in the gall pipe mouth, can play the effect of bile filter, can adsorb the tiny gallstone granule in the bile in the net free, wait to adsorb after the certain time, can suspend in the bile along with the increase of weight, the gallstone of initial stage both has been realized and has been collected fixedly, can not influence the normal discharge of bile again, the adhesion of gallstone granule has been improved greatly, compare than traditional bezoar bed, the agglutination rate of gallstone is improved to more than 65% by less than 30% of traditional bezoar bed in the bile, improve the output of bezoar greatly.
The foregoing shows and describes the general principles and features of the present invention, together with the advantages thereof. It will be appreciated by those skilled in the art that the present invention is not limited by the embodiments described above, which are presented in the foregoing description for the purpose of illustrating the principles of the invention and not by the purpose of limiting the invention. The present invention is susceptible to various changes and modifications which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (4)
1. The three-dimensional compound bezoar in vivo cultivation method is characterized by comprising the following steps:
s1, implanting the cattle into a bezoar bed, and selecting 6-month-old bred cattle to perform conventional cholecystotomy;
s2: implanting bezoar bacteria into the gallbladder of the cattle;
s3, feeding, wherein conventional forage grass is used in the breeding process;
the feeding principle of big and small adjustment is adopted, the total feeding amount is unchanged, but the feeding method of indefinite and indefinite amount and the feeding after 30 minutes of small-dose feeding are adopted, and the secretion of bile is induced to increase by utilizing the principle that food reflects to the stomach and intestine, so that more bezoar is formed;
s4, feeding by adding additives, wherein self-prepared additives are periodically added in the feeding process;
the additive is a natural plant additive prepared from herba Lysimachiae Christinae, stigma Maydis, herba Artemisiae Scopariae, etc., and can promote bile secretion, accelerate calculus bovis generation, and reduce the influence of cholelithiasis on normal physiological function of cattle;
s5, taking yellow, slaughtering when finishing breeding and marketing, taking out the gall bladder, rapidly sucking excess gall from the gall by using materials such as absorbent paper and the like, drying in a drying oven at 50 ℃, cleaning out a bezoar bed and other impurities, and sealing and storing in a colored glass bottle;
the bezoar bacteria is an escherichia coli strain separated from the gall bladder of white feather broiler chicken, and can survive for a long time after being planted in the gall bladder of cattle after being adapted to the environment of the gall bladder of cattle through culture of a culture medium, and the generated inflammatory reaction is slight and lasting;
the bezoar bed is of a net structure made of nylon materials, and the two monomers are overlapped, so that the filtering area is doubled, the filtering of bile salt particles can be improved, and the bile salt which is filtered can be reduced from falling off again;
the nylon filter screen is additionally provided with the cylindrical net-shaped metal cover, so that the filtering area can be increased, the stability of the bezoar bed is increased, and the bezoar bed can be prevented from being extruded and deformed or even being extruded and flattened by the contraction of the gallbladder when the gallbladder is empty;
the combination of the inner and outer layers makes the density of the whole bed close to and slightly lower than that of bile, so that the bed can be suspended in the bile, and the bed is completely wrapped by the bezoar because the center of the bed is not filled with a solid body.
2. The method for cultivating calculus bovis in vivo as claimed in claim 1, wherein: in the step S1, the specific operation is as follows:
feeding is forbidden in the first 24 hours, and water is forbidden in 8 hours;
performing surgical disinfection and anesthesia, wherein a surgical incision is formed between the reciprocal 2 and 3 ribs of the right body wall of a young cow, the lower edge of the longissimus dorsi and the midpoint of the upper edge of 11 costal arches and is 8 cm long, and the skin, the external oblique muscles, the internal oblique muscles and the peritoneum are surgically cut to find a gallbladder;
if the gallbladder is full, a part of bile is extracted by the injector, a disposable full-layer incision about 1.5 cm is made on the gallbladder, a bezoar bed is placed, 1ml of diluted bacterium solution is dripped, full-layer spiral continuous suture is made on the first layer of the gallbladder, and vertical inverted continuous suture of a serous-muscular layer is made on the second layer of the gallbladder, so as to ensure that the bile does not leak;
suturing abdominal muscle and skin, performing postoperative care for 1 week, removing skin suture line after 8 days, and recovering conventional breeding.
3. The method for cultivating calculus bovis in vivo as claimed in claim 1, wherein: the end of the breeding is about 30 months old.
4. The method for cultivating calculus bovis in vivo as claimed in claim 2, wherein: 1ml of diluted bacteria liquid containing more than 50 hundred million live bacteria is dropped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210870068.XA CN115067275B (en) | 2022-07-22 | 2022-07-22 | Three-dimensional compound bezoar cultivation method in vivo |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210870068.XA CN115067275B (en) | 2022-07-22 | 2022-07-22 | Three-dimensional compound bezoar cultivation method in vivo |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115067275A CN115067275A (en) | 2022-09-20 |
| CN115067275B true CN115067275B (en) | 2022-11-04 |
Family
ID=83242125
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210870068.XA Active CN115067275B (en) | 2022-07-22 | 2022-07-22 | Three-dimensional compound bezoar cultivation method in vivo |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115067275B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116392509A (en) * | 2023-06-08 | 2023-07-07 | 内蒙古为旭生物科技有限公司 | Method for inducing cow to form bezoar |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87100158A (en) * | 1987-01-16 | 1987-08-12 | 新疆石河子农学院 | The cultivating artificial calculus bovis |
| CN1040907A (en) * | 1988-09-08 | 1990-04-04 | 汪立新 | Artificial bezoar-cultivation with get the bile new method |
| CN1050985A (en) * | 1989-10-20 | 1991-05-01 | 山东农业大学 | Rapid cultivating bezoar and biliary drainage device and method |
| JP2005075763A (en) * | 2003-08-29 | 2005-03-24 | Daicho Kikaku:Kk | Drug for animal |
| TW201006480A (en) * | 2008-08-12 | 2010-02-16 | Nat Univ Chung Hsing | A manufacturing method of in vitro cultured Calculus Bovis |
| CN102429927A (en) * | 2011-12-21 | 2012-05-02 | 武汉大鹏药业有限公司 | Method for preparing ox gal stone |
| CN105288315A (en) * | 2015-12-04 | 2016-02-03 | 青岛安倍康生物医药技术有限公司 | Christina loosestrife herb oral liquid for clearing away heat, removing dampness, benefiting bile and expelling toxin and preparation method of christina loosestrife herb oral liquid |
| CN106063840A (en) * | 2016-05-24 | 2016-11-02 | 南京泽朗医药技术有限公司 | A kind of choleretic Chinese medicine composition |
| CN110959574A (en) * | 2019-10-15 | 2020-04-07 | 太湖县华中农业发展有限公司 | Feeding method for promoting intestinal health of suckling piglets |
| CN111184589A (en) * | 2020-02-27 | 2020-05-22 | 四川农业大学 | Detachable bezoar bed and bezoar cultivation method |
| CN111939177A (en) * | 2019-04-30 | 2020-11-17 | 千芝堂生命科学(深圳)有限公司 | Combined medicine for being implanted into gallbladder, gallbladder implant containing combined medicine and application of gallbladder implant in-vivo cultivation of bezoar |
-
2022
- 2022-07-22 CN CN202210870068.XA patent/CN115067275B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87100158A (en) * | 1987-01-16 | 1987-08-12 | 新疆石河子农学院 | The cultivating artificial calculus bovis |
| CN1040907A (en) * | 1988-09-08 | 1990-04-04 | 汪立新 | Artificial bezoar-cultivation with get the bile new method |
| CN1050985A (en) * | 1989-10-20 | 1991-05-01 | 山东农业大学 | Rapid cultivating bezoar and biliary drainage device and method |
| JP2005075763A (en) * | 2003-08-29 | 2005-03-24 | Daicho Kikaku:Kk | Drug for animal |
| TW201006480A (en) * | 2008-08-12 | 2010-02-16 | Nat Univ Chung Hsing | A manufacturing method of in vitro cultured Calculus Bovis |
| CN102429927A (en) * | 2011-12-21 | 2012-05-02 | 武汉大鹏药业有限公司 | Method for preparing ox gal stone |
| CN105288315A (en) * | 2015-12-04 | 2016-02-03 | 青岛安倍康生物医药技术有限公司 | Christina loosestrife herb oral liquid for clearing away heat, removing dampness, benefiting bile and expelling toxin and preparation method of christina loosestrife herb oral liquid |
| CN106063840A (en) * | 2016-05-24 | 2016-11-02 | 南京泽朗医药技术有限公司 | A kind of choleretic Chinese medicine composition |
| CN111939177A (en) * | 2019-04-30 | 2020-11-17 | 千芝堂生命科学(深圳)有限公司 | Combined medicine for being implanted into gallbladder, gallbladder implant containing combined medicine and application of gallbladder implant in-vivo cultivation of bezoar |
| CN110959574A (en) * | 2019-10-15 | 2020-04-07 | 太湖县华中农业发展有限公司 | Feeding method for promoting intestinal health of suckling piglets |
| CN111184589A (en) * | 2020-02-27 | 2020-05-22 | 四川农业大学 | Detachable bezoar bed and bezoar cultivation method |
Non-Patent Citations (3)
| Title |
|---|
| 人工培植牛黄的方法;李晓坤;《饲料研究》;19950127(第01期);全文 * |
| 南阳黄牛人培牛黄技术试验报告;杨前锋等;《中国农学通报》;20060628(第06期);全文 * |
| 形形色色的人工培植牛黄实例;董建平等;《中国兽医杂志》;19990222(第02期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115067275A (en) | 2022-09-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101595851B (en) | Method for breeding crabs and controlling diseases | |
| CN104509490B (en) | Simulated ecological breeding method for Chinese soft-shelled turtles | |
| CN104688780B (en) | A kind of caprophyl liquid applied to prevention and treatment grice diarrhoea | |
| CN109548717A (en) | A kind of Micropterus salmonoides seed method for massively culturing | |
| CN101627747A (en) | Method for hatching leeche and raising seedling of leeche | |
| CN115067275B (en) | Three-dimensional compound bezoar cultivation method in vivo | |
| CN101863704A (en) | Pleurotus eryngii culture medium and culture method thereof | |
| CN107711616A (en) | A kind of tame and docile food method of mandarin fish seed | |
| CN106614162A (en) | High-yield aquaculture method for river snails | |
| CN105994047A (en) | Ecological selenium-enriched soft-shelled turtle aquaculture technique | |
| CN101341857A (en) | Ecological regulate and control cultivation method for penaeus orientalis in sea water pool | |
| CN106635816B (en) | A kind of algae mixed culturing method | |
| CN103004662A (en) | Method for cultivating loach water flower seedlings by using fresh water benthic diatoms | |
| CN106630088A (en) | Preparation for preventing and treating citrus yellow shoot and preparation method of preparation | |
| CN102972294A (en) | Rapid honeysuckle tissue culture propagation method | |
| CN110663642A (en) | Method for changing intestinal bacterial colony of sick chicken by using excrement transplantation | |
| CN107873594A (en) | The cultivation of crab and the prevention and controls of disease | |
| CN112385581A (en) | Method for hatching and breeding leeches in saline-alkali water area | |
| CN106665436A (en) | Method of transplanting healthy fish intestinal feces and treating ill fishes | |
| CN109006606A (en) | A kind of anti-mud cray method for breeding | |
| CN102090354A (en) | Method for artificially culturing corbicula fluminea | |
| CN217523642U (en) | Copper fry breeding pond | |
| CN108849728A (en) | A kind of free-ranging method for fattening black pig | |
| CN107006424A (en) | A kind of wild Rana boulengeri chartering cost method | |
| CN216392024U (en) | Purple rice is planted with seed selection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |