CN115058245A - Green fluorescent carbon quantum dot and application thereof in rapid detection of allura red - Google Patents
Green fluorescent carbon quantum dot and application thereof in rapid detection of allura red Download PDFInfo
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- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/15—Nano-sized carbon materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
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- C01P2004/00—Particle morphology
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- G—PHYSICS
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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Abstract
The invention discloses a green fluorescent carbon quantum dot and application thereof in the aspect of rapid detection of allura red, wherein 4-aminobenzoic acid (PABA) and m-phenylenediamine are used as precursors, a green fluorescent carbon quantum dot (G-FCNs) is prepared by a hydrothermal synthesis method, and the green fluorescent carbon quantum dot is used as a probe for ultra-sensitive detection of AR. The invention obviously improves the sensitivity of the FCNs probe to AR detection and opens up a new way for AR rapid detection. The detection limit of the AR rapid detection method based on the G-FCNs established by the invention is as low as 23.5nM, so that the AR rapid detection method has high sensitivity. In addition, the method has the advantages of simple operation, low cost and quick analysis, and can be used for analyzing actual samples, thereby having good application and popularization values.
Description
Technical Field
The invention relates to the technical field of rapid detection of allura red, and particularly relates to a green fluorescent carbon quantum dot and application thereof in rapid detection of allura red.
Background
The synthetic pigment has the advantages of bright color, low cost and strong dyeing power, and is widely used in the food industry to change the appearance of food, thereby improving appetite. Allure Red (AR) belongs to synthetic azo dyes, has the advantages of low cost and high stability, and is widely used in various foods, such as candies, jellies, juices, dairy products, beverages and the like. Despite its wide application, its toxicity and pathogenicity are not negligible due to its unique structure with azo functional groups and aromatic rings. It has been reported that excessive use of AR may cause various diseases such as respiratory and digestive problems, hyperactivity, insomnia, asthma, allergy, chromosome damage, lymphoma, and the like. To prevent AR abuse, regulations are enacted in many countries. For example, in the united states, germany, norway, denmark, france, sweden, switzerland, belgium, austria and indian AR are classified as illegal food colorants and are completely prohibited from use. China specifies the maximum permitted usage of AR in different types of food, for example, the maximum permitted usage of AR in soft drinks is 0.1g kg -1 The maximum allowable usage limit of the candies and the chocolate is 0.3g kg -1 . Therefore, there is a need to develop a simple, rapid and convenient analytical method for accurately determining the AR content in food.
Currently, researchers have established a variety of analytical methods to accurately detect AR, including Thin Layer Chromatography (TLC), Differential Pulse Polarography (DPP), electrochemical sensing, Capillary Electrophoresis (CE), High Performance Liquid Chromatography (HPLC), and the like. While these methods have proven useful for AR detection, they typically suffer from expensive, cumbersome sample preparation procedures and long time consuming instrumentation. Therefore, the fluorescence method based on the functionalized carbon quantum dots (FCNs) has the advantages of rapidness, simplicity in operation, low cost, good selectivity, high sensitivity and the like, and shows a good application prospect in the aspect of overcoming the difficulties.
Carbon quantum dots(FCNs) generally refer to oxygen-enriched carbon nanoparticles having a particle size of less than 10nm, with pronounced fluorescence (PL) properties. FCNs have excellent photostability, adjustable emission, water solubility, biocompatibility, and low cytotoxicity. Based on these advantages, FCNs have occupied a niche in food testing. Research shows that the FCNs-based sensing system has higher sensitivity in the aspect of detecting food additives such as lemon yellow, citric acid, malachite green, carmine, glutathione and the like. However, to date, research into AR detection using FCNs as nanoprobes is still rare and only two reports have been found. Vijeata et al prepared three FCNs probes for AR detection using fruit shell, pulp and gum mixtures with detection limits of 0.260, 0.607 and 0.166. mu. mol L, respectively -1 . In another study, Gunjal et al prepared FCNs probes for AR detection from sawmill waste as a raw material with an obtained detection limit of 0.91. mu. mol L -1 (0.45μg mL -1 ). These two studies demonstrate the feasibility of using FCNs as nanoprobes for AR detection. However, both of these works are based on FCNs that fluoresce in the blue at short wavelengths, and are susceptible to interference from solvent scattering spectra or lack of large stokes shifts limiting the sensitivity of detection. To further improve the analytical performance of FCNs-based fluorescence methods, it is essential to develop FCNs probes based on long-wavelength fluorescence for AR detection.
Disclosure of Invention
Aiming at the problem of insufficient sensitivity of the existing AR detection technology based on short-wavelength blue fluorescent FCNs, the invention develops a rapid detection method based on green fluorescent G-FCNs for AR detection, and the sensitivity of the rapid detection method is far higher than that of any existing fluorescent probe based on FCNs. In addition, the method has the advantages of simple operation, low cost and quick detection, and can be used for AR detection in actual food matrixes.
The invention adopts the following technical scheme:
a preparation method of green fluorescent carbon quantum dots comprises the following steps:
(1) dissolving 0.13g of 4-aminobenzoic acid and 0.10g of m-phenylenediamine in 20.0mL of a mixed solution of ultrapure water and absolute ethyl alcohol at an equal volume ratio; transferring the mixture solution into a polytetrafluoroethylene-lined autoclave, and heating at a constant temperature of 180 ℃ for 12 hours;
(2) the reaction product was allowed to stand to cool to room temperature and then transferred to a 50.0mL centrifuge tube. After centrifugation at 12000rpm/min for 15 minutes, the supernatant was collected;
(3) redissolving the obtained supernatant in ultrapure water, and purifying in a 1.0L beaker through a dialysis membrane for 3 days, and supplementing fresh ultrapure water every 24 h; and (4) freeze-drying the purified solution containing the G-FCNs to obtain dried G-FCNs powder, and storing the dried G-FCNs powder in a drying box for a long time.
The application of the green fluorescent carbon quantum dots in the aspect of rapid detection of allura red comprises the following steps:
1) mixing ultrapure water and green fluorescent carbon quantum dots to obtain a green fluorescent carbon quantum dot solution;
2) respectively mixing and reacting the allure red with different concentrations with the green fluorescent carbon quantum dot solution to obtain mixed solutions with different allure red concentrations, respectively measuring the fluorescence intensity of the mixed solutions with different allure red concentrations, and obtaining the fluorescence quenching efficiency F 0 Linear relationship of/F and allura red concentration in the mixed solution;
3) mixing a sample to be detected with the green fluorescent carbon quantum dot solution for reaction to obtain a mixed solution of the sample to be detected, and measuring the fluorescence intensity of the mixed solution of the sample to be detected;
4) according to fluorescence quenching efficiency F 0 And obtaining the concentration of the allura red in the mixed solution of the sample to be detected through the linear relation between the concentration of the allura red in the mixed solution and the concentration of the allura red in the mixed solution.
Further, the concentration of the green fluorescent carbon quantum dot solution in the step 1) is 0.001-0.1mg mL -1 。
Further, the concentration of the green fluorescent carbon quantum dot solution in the step 1) is 0.01mg mL -1 。
Further, step 2) the fluorescence quenching efficiency F 0 The linear relationship between/F and allura red concentration in the mixed solution is: y 0.0849x + 0.9883.
Further, in the step 2) and the step 3), the pH value of the reaction system is between 2.0 and 12.0.
Further, in the step 2) and the step 3), the pH value of the reaction system is 6.5.
Further, in the step 2) and the step 3), the fluorescence intensity is measured at lambda ex Is 400nm, lambda em Measured at 493 nm.
Further, the reaction time of the step 2) and the step 3) is 1 min.
Further, in step 3), the sample to be tested is: soft drink, candy or chocolate containing AR is pulverized, dissolved or diluted, and filtered with cellulose acetate membrane injection filter with pore diameter of 0.45 μm to obtain extractive solution.
The invention has the following beneficial effects:
the invention takes 4-aminobenzoic acid (PABA) and m-phenylenediamine as precursors, prepares the green fluorescent G-FCNs by a hydrothermal synthesis method, takes the green fluorescent G-FCNs as probes for the ultra-sensitive detection of AR, has the detection limit as low as 23.5nM, and opens up new prospects for improving the detection performance of AR detection. The method also has the advantages of simple operation, low cost and quick analysis, and can be used for analyzing actual samples, thereby having good application and popularization values.
Drawings
FIG. 1 is a schematic view of the detection method of the present invention;
FIG. 2(A) UV-visible absorption spectra (solid line), fluorescence excitation spectra (dashed line) and emission spectra (dotted line) of G-FCNs; the insert picture at the upper right corner is an ultraviolet-visible absorption spectrum amplified in the range of 300-500nm, and the picture at the upper left corner is a G-FCNs aqueous solution under the irradiation of natural light (left) and ultraviolet light (right); (B) fluorescence spectra of G-FCNs at different excitation wavelengths; (C) (a) quinine sulfate and (b) a G-FCNs ultraviolet absorption intensity-fluorescence spectrum peak area integral diagram;
FIG. 3 is an (A) TEM image of G-FCNs; (B) a particle size distribution histogram; (C) an FTIR spectrogram;
FIG. 4(A) graph of the effect of pH on detecting AR; (B) a graph of the effect of G-FCNS concentration on detecting AR; (C) the influence curve of the reaction time on the detection of AR;
FIG. 5(A) contains different concentrations of AR (0.0-60.0. mu. mol L) -1 ) Solution of G-FCNs (0.01mg mL) -1 ) A fluorescence spectrum of (a); (B) f 0 A linear plot of/F versus AR concentration;
FIG. 6 is a bar graph of (A) selectivity and (B) interference immunity of G-FCNs to small molecules such as AR, anions and cations, and various amino acids.
Detailed Description
In order to more clearly illustrate the technical solution of the present invention, the following examples are given. The starting materials, the reactions and the work-up procedures present in the examples are, unless otherwise stated, commercially available starting materials and techniques known to those skilled in the art.
As shown in FIG. 1, G-FCNs were prepared by hydrothermal heating of 4-aminobenzoic acid (PABA) and m-phenylenediamine. AR effectively reduces the fluorescence intensity of G-FCNs in a concentration-dependent manner through electrostatic adsorption, Internal Filter Effect (IFE) and dynamic quenching. Therefore, a fluorescence method based on G-FCNs is proposed to detect AR. The influence of detection conditions such as G-FCNs concentration, pH value and reaction time on the analysis performance is studied in detail. The method is thoroughly verified by researching the linear range, the detection limit, the selectivity and the anti-interference capability of the method. Finally, the method is used for determining AR in food to evaluate the feasibility of practical application. The method has ultrahigh sensitivity, good selectivity, low cost and easy operability, and provides a convenient and reliable analysis strategy for AR monitoring.
Synthesis of G-FCNs:
0.13g of PABA and 0.10g of m-phenylenediamine were dissolved in 20.0mL of a mixed solution of ultrapure water and absolute ethanol (1:1v: v). The mixture solution was transferred to a polytetrafluoroethylene-lined autoclave and heated at a constant temperature of 180 ℃ for 12 hours. The reaction product was allowed to stand to cool to room temperature and then transferred to a 50.0mL centrifuge tube. After centrifugation at 12000rpm (revolutions per minute) for 15 minutes, the supernatant was collected, redissolved in ultrapure water, and purified by dialysis membrane (MWCO ═ 500-. The purified solution containing the G-FCNs is lyophilized to obtain dried G-FCNs powder, which is stored in a drying oven for a long time.
Physical and chemical property characterization of G-FCNs:
the ultraviolet absorption properties of G-FCNs were investigated using ultraviolet-visible absorption spectroscopy (UV-Vis). FIG. 2A (solid line) shows UV-Vis spectra of G-FCNs with absorption peaks at 240, 270 and 375 nm. The peaks at 240nm are pi → pi transitions due to C ═ N bonds, while the peaks at 270 and 375nm are N → pi transitions due to C ═ O bonds.
The fluorescence properties of G-FCNs were investigated by fluorescence spectroscopy. As shown in FIG. 2A (dashed line), G-FCNs have an excitation peak at 400 nm. As shown in FIG. 2A (dotted line), the emission peak of G-FCNs at 400nm excitation was 493 nm. Aqueous solutions of G-FCNs appear brownish yellow in natural light (FIG. 2A, left of the top left inset) and bright green under UV irradiation (FIG. 2A, right of the top left inset). FIG. 2B is an emission spectrum of G-FCNs at different excitation wavelengths, which is seen to exhibit excitation wavelength dependence. As shown in FIG. 2C, the quantum yield of G-FCNs was measured to be 15.40% using quinine sulfate as a reference material.
Morphology and size of the G-FCNs were measured by TEM method. As shown in FIG. 3A, the G-FCNs are uniform in size and spherical in shape. FIG. 3B is a histogram of the particle size distribution of G-FCNs. The grain size distribution range of the G-FCNs is 0.35-6.65nm, and the average grain size is 2.5 +/-0.4 nm.
The functional groups of the G-FCNs are characterized by Fourier infrared spectroscopy. As shown in FIG. 3C, at 3426 and 3213cm -1 The absorption peak in the range is attributed to O-H/N-H stretching vibration. 2923cm -1 And 1693cm -1 The absorption peaks at (a) correspond to the stretching vibration of C-H and C ═ O, respectively. The C-N and N-H stretching vibration respectively appears at 1600cm -1 And 1496cm -1 . The C-N stretching vibration and the C-O stretching vibration respectively appear in 1313-1260cm -1 And 1170cm -1 . Thus, the hydrophilicity of G-FCNs is attributed to the unsaturated carbonaceous structure having nitrogen-and oxygen-containing functional groups on its surface.
3. Optimization of experimental conditions
In order to obtain the best detection performance, main working parameters of the sensing platform are optimized, wherein the main working parameters comprise the pH of a reaction system, the concentration of the G-FCNs solution and the reaction.
3.1 Effect of pH on the assay
As shown in FIG. 4A, pH value(PBS solution, 10.0mM) varying between 2.0 and 12.0 results in a fluorescence quenching efficiency, F 0 /F (wherein F) 0 And F respectively refer to fluorescence intensities of G-FCNs before and after mixing with AR), and the highest value was obtained at pH 6.5 (ultrapure water). Therefore, the optimum pH was selected to be 6.5. In order to simplify the steps, ultrapure water is adopted as a solution in the subsequent detection work.
Effect of 3.2G-FCNs concentration on detection
As shown in FIG. 4B, the concentration of G-FCNs increased from 0.001 to 0.01mg mL -1 Time of day, F of the sensing system 0 the/F increased significantly, in the higher concentration range (0.01-0.1mg mL) -1 ) Inner F 0 the/F drops sharply. Therefore, 0.01mg mL was selected -1 The optimum concentration of G-FCNs is obtained.
3.3 Effect of reaction time on the assay
As shown in FIG. 4C, F 0 the/F sharply increases within 1.0min and tends to be constant along with the time extension, which indicates that the sensing system has high reaction speed and can complete the reaction only within 1.0min, therefore, 1.0min is adopted as the optimal reaction time in the subsequent detection work.
4. Analytical method validation
4.1 Linear Range
To investigate the effect of AR on the fluorescence intensity of G-FCNs, a concentration of 0.01mg mL was prepared using ultrapure water -1 Then adding AR with different concentrations into the prepared G-FCNs solution, reacting for 1.0min, and recording the fluorescence intensity of the mixed solution under the excitation of 400nm and the emission of 493 nm.
As shown in FIG. 5A, in a solution of G-FCNs (0.01mg mL) -1 ) After addition of different concentrations of AR, a gradual decrease in the fluorescence intensity of the G-FCNs was observed with increasing AR concentration. When the AR concentration reaches 60.0 mu mol L -1 At this time, the fluorescence of G-FCNs was quenched by approximately 95.87%. Indicating that AR can effectively quench the fluorescence intensity of G-FCNs. As shown in FIG. 5B, fluorescence quenching efficiency F 0 The dependence of/F on AR concentration is linear. The corresponding regression equation is 0.0849x + 0.9883. The detection limit was calculated to be 23.5nmol L -1 . The detection limits of the present detection method were compared to reported fluorescent methods based on FCNs, as shown in Table 1As shown, the sensitivity of the present detection method is much higher than that of any existing FCNs-based fluorescence detection method.
TABLE 1 detection Performance of FCNs-based AR fluorometry
4.2 Selectivity
This work investigated the selectivity of G-FCNs over AR. Mixing AR with different kinds of interfering substances including cation (K) + 、Na + 、Fe 2+ 、Ba 2+ 、Pb 2+ 、Cd 2+ And Mg 2+ ) Anion (Br) - 、H 2 PO 4 2- 、S 2 O 3 2- 、Cl - 、NO 3 - 、NO 2 - And F - ) Pigments (amaranth, indigo and quinoline yellow) and other small molecules (serine, tyrosine, glycine, tryptophan, glucose, D-fructose and sucrose) were dissolved in ultrapure water to a final concentration of 0.1mmol L -1 . 2.0. mu.L of each was added to a series of 5.0mL solutions containing 2.0mL of G-FCNs (0.01mg mL of each solution) -1 ) In the centrifuge tube. After thorough mixing, the maximum fluorescence intensity of the mixed solution at 493nm was recorded at an excitation wavelength of 400 nm.
As shown in fig. 6A, the introduction of AR resulted in a sharp decrease in the fluorescence intensity of G-FCNs, while other interfering substances that may be present had little significant effect on the fluorescence intensity of G-FCNs, indicating that the detection method proposed in this work has excellent selectivity for AR detection.
4.3 interference immunity
This work examined the interference immunity of G-FCNs to AR detection. A series of 20.0. mu.L AR solutions (10.0mmol L) -1 ) Added to a solution containing 2.0mL of G-FCNs (0.01mg mL) -1 ) In a 5.0mL centrifuge tube. Then, 2.0. mu.L of each of the above-mentioned interfering substances was added to a final concentration of 0.1mmol L -1 . Similar to the selectivity study, the fluorescence intensity at 493nm under excitation at 400nm of the mixture solution was recorded.
As shown in FIG. 6B, the fluorescence of the G-FCNs/AR system is not interfered by the foreign substances, which shows that the method has good anti-interference capability. Therefore, the proposed method has the potential to be used for AR determination in real food samples.
5. Actual sample testing
Different kinds of food samples, such as AR-containing soft drinks, candies and chocolates, were purchased from local markets. For beverage samples, they were first boiled to remove dissolved CO 2 Then, 2.0mL of the sample was transferred to a centrifuge tube and diluted with 10.0mL of ultrapure water. For candies and sugar coated chocolates, they were first pulverized into fine powders, 0.1g of each sample powder was dissolved in 10.0mL of ultrapure water and sonicated for 10.0 min. Finally, the sample extract was filtered through a cellulose acetate membrane syringe filter having a pore size of 0.45 μm. A solution of G-FCNs (0.01mg mL) -1 ) Transfer to 5.0mL centrifuge tubes each containing 2.0mL of G-FCNs solution. Mixing with 20.0 μ L of each sample extractive solution, and measuring the fluorescence intensity of G-FCNs (i.e. F) at 493nm before and after adding the sample extractive solution at excitation wavelength of 400nm 0 And F).
TABLE 2 detection of AR in food samples
As shown in Table 2, the AR contents in the above food samples were 0.30, 0.71, 0.22, 0.42 and 0.51. mu. mol L, respectively -1 Corresponding to 0.14, 0.34, 0.11, 0.20 and 0.24mg kg, respectively -1 All below the maximum limit allowed for AR use in soft drinks, candies and chocolate samples in our country and therefore can be safely consumed. Sample recovery tests showed recoveries in the range of 97.4-104.8% with Relative Standard Deviation (RSD) less than 3.58%. In addition, by comparison of HPLC analysis, it was observed that the detection results of the two methods were consistent, indicating that the proposed detection method had high accuracy.
Furthermore, it should be understood that although the present specification describes embodiments, not every embodiment includes only a single embodiment, and such description is for clarity purposes only, and it is to be understood that all embodiments may be combined as appropriate by one of ordinary skill in the art to form other embodiments as will be apparent to those of skill in the art from the description herein.
Claims (10)
1. A preparation method of green fluorescent carbon quantum dots is characterized by comprising the following steps:
(1) dissolving 0.13g of 4-aminobenzoic acid and 0.10g of m-phenylenediamine in 20.0mL of a mixed solution of ultrapure water and absolute ethanol in an equal volume ratio; transferring the mixture solution into a polytetrafluoroethylene-lined autoclave, and heating at a constant temperature of 180 ℃ for 12 hours;
(2) the reaction product was allowed to stand to cool to room temperature and then transferred to a 50.0mL centrifuge tube. After centrifugation at 12000rpm/min for 15 minutes, the supernatant was collected;
(3) redissolving the obtained supernatant in ultrapure water, and purifying in a 1.0L beaker through a dialysis membrane for 3 days, and supplementing fresh ultrapure water every 24 h; the purified solution containing the G-FCNs is lyophilized to obtain dried G-FCNs powder, which is stored in a drying oven for a long time.
2. The application of the green fluorescent carbon quantum dots in the aspect of rapid detection of allura red as claimed in claim 1, is characterized by comprising the following steps:
1) mixing ultrapure water and green fluorescent carbon quantum dots to obtain a green fluorescent carbon quantum dot solution;
2) respectively mixing and reacting the allure red with different concentrations with the green fluorescent carbon quantum dot solution to obtain mixed solutions with different allure red concentrations, respectively measuring the fluorescence intensity of the mixed solutions with different allure red concentrations, and obtaining the fluorescence quenching efficiency F 0 Linear relationship of/F and allura red concentration in the mixed solution;
3) mixing a sample to be detected with the green fluorescent carbon quantum dot solution for reaction to obtain a mixed solution of the sample to be detected, and measuring the fluorescence intensity of the mixed solution of the sample to be detected;
4) according to fluorescence quenching efficiency F 0 And obtaining the concentration of the allura red in the mixed solution of the sample to be detected through the linear relation between the concentration of the allura red in the mixed solution and the concentration of the allura red in the mixed solution.
3. The use of claim 2, wherein the concentration of the green fluorescent carbon quantum dot solution in the step 1) is 0.001-0.1mg mL -1 。
4. The use of claim 2, wherein the concentration of the green fluorescent carbon quantum dot solution in the step 1) is 0.01mg mL -1 。
5. The use of claim 2, wherein the fluorescence quenching efficiency F of step 2) is 0 The linear relationship between/F and allura red concentration in the mixed solution is as follows: y 0.0849x + 0.9883.
6. The use according to claim 2, wherein in step 2) and step 3), the reaction system has a pH between 2.0 and 12.0.
7. The use according to claim 2, wherein in step 2) and step 3), the reaction system has a pH of 6.5.
8. The use according to claim 2, wherein in step 2) and step 3) the fluorescence intensity is measured at λ ex Is 400nm, lambda em Measured at 493 nm.
9. The use of claim 2, wherein the reaction time in step 2) and step 3) is 1 min.
10. The use of claim 2, wherein the sample to be tested in step 3) is: soft drink, candy or chocolate containing AR is pulverized, dissolved or diluted, and filtered with cellulose acetate membrane injection filter with pore diameter of 0.45 μm to obtain extractive solution.
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