CN115053900A - Bactericide for preventing and treating avocado canker - Google Patents

Bactericide for preventing and treating avocado canker Download PDF

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Publication number
CN115053900A
CN115053900A CN202210829665.8A CN202210829665A CN115053900A CN 115053900 A CN115053900 A CN 115053900A CN 202210829665 A CN202210829665 A CN 202210829665A CN 115053900 A CN115053900 A CN 115053900A
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carvone
canker
avocado
bactericide
preventing
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CN202210829665.8A
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Chinese (zh)
Inventor
周海兰
唐桓伟
周俊岸
庞新华
潘祖建
朱鹏锦
郝小玲
唐秀观
欧景莉
杜英俊
钟云婕
唐圣理
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Guangxi Subtropical Crops Research Institute
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Guangxi Subtropical Crops Research Institute
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to the technical field of pesticides, and particularly relates to a bactericide for preventing and treating avocado canker. The effective component of the bactericidal composition is formed by compounding levo-carvone and diniconazole, tebuconazole or triadimefon. When 2 effective components in the bactericidal composition and the bactericide are compounded, the co-toxicity coefficient is more than 120 within a certain mass range, and the synergistic effect type is shown for preventing and treating the avocado canker. Compared with any single component contained in the pesticide composition, the pesticide composition can improve the control effect on the avocado canker, and on the basis, the application dosage of the active component can be reduced, the pesticide residue is reduced, and the environmental stress is relieved.

Description

Bactericide for preventing and treating avocado canker
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to a bactericide for preventing and treating avocado canker.
Background
Avocados, also known as avocados, are cultivated in the places of China, such as Guangdong, Guangxi, Hainan, Taiwan and Sichuan provinces. The avocado is a new nutritional health-care fruit, has yellow pulp and unique flavor, and is rich in multiple vitamins, unsaturated fat, high protein, low sugar, sodium, potassium, magnesium and other mineral nutrients. Avocado canker is a disease caused by the infestation of avocado canker pathogens (dothiella sp.). The main trunk, main branch and branch of the avocado are mainly harmed by avocado canker, and most of scabs appear at wounds, skin holes, leaf marks and branch bifurcations. The disease spots in the early stage of the disease are in the shape of water spots, dark black or black brown, and gradually form long spindle shapes, oval shapes or irregular shapes, and the sizes of the disease spots are related to the disease onset time. If the lesion is surrounded by the branch for a circle, the branches and leaves on the upper part of the lesion slowly dry up. In the late stage of the disease, the lesion surrounds the trunk for a week, hindering water and nutrient transport, thereby causing the death of the whole plant.
At the present stage, the prevention and treatment measures for the avocado canker mainly comprise spraying of chemical agents, the chemical agents can directly act on targeted germs, and the effect is fast. However, in the aspect of disease control, long-term application of single-component chemical agents easily causes pathogenic bacteria to generate drug resistance, and once the drug resistance is generated, the disease control effect is reduced; in order to improve the control effect, farmers generally increase the application dosage of pesticides, which leads to increase of pesticide residues and environmental stress. Therefore, there is a need to develop new chemical agents.
L-carvone, namely spearmint oil and 2, 3-dimethyl-5-propenyl-cyclohexanone-1, is a light yellow clear liquid and is insoluble in water. L-carvone is the main component of volatile oil extracted from Mentha spicata of Mentha of Labiatae. The volatile oil of spearmint and the main component of the levocarvone thereof have the bacteriostatic activity on various plant pathogenic fungi, and have the prospect of being developed into chemical agents for preventing and treating plant diseases. Application No. CN201811396252.5 discloses a levo-carvone aerosol which can be used for preventing and treating plant diseases caused by plant pathogenic bacteria (orchid anthracnose, peony gray mold and the like). In addition, the application number CN201811536166.X also discloses a sterilization synergistic composition for preventing and treating tomato gray mold, which is prepared by compounding levo-carvone with diethofencarb, boscalid or pyraclostrobin, has obvious synergistic effect on tomato gray mold, improves the control effect, and is also favorable for delaying the generation of drug resistance of pathogenic bacteria.
The compounding of different pesticide effective components is an effective and rapid way for developing new pesticide products and preventing and treating resistant germs at present. The screening of the compound pesticide with the synergistic effect can obviously improve the control effect, reduce the pesticide use amount, reduce the control cost, delay the generation of germ resistance and reduce the environment. The inventor discovers that the L-carvone has a synergistic effect when being compounded with diniconazole, tebuconazole or triadimefon through an indoor test, and no relevant report is found at present.
Disclosure of Invention
The invention aims to provide a bactericide for preventing and treating avocado canker, which is prepared by compounding levo carvone and other effective components, generates a synergistic interaction effect, can improve the prevention and treatment effect on avocado canker bacteria, and simultaneously can provide support for developing novel chemical agents.
In order to achieve the purpose, the invention provides the following technical scheme:
the invention aims to provide a bactericidal composition for preventing and treating avocado canker, which is prepared by compounding the effective components of levocarvone and diniconazole, tebuconazole or triadimefon.
Preferably, the mass ratio of the levocarvone to the diniconazole is 1-12: 20-1.
Preferably, the mass ratio of the levocarvone to the tebuconazole is 1-40: 30-1.
Preferably, the mass ratio of the levocarvone to the triazolone is 1-60: 15-1.
The second purpose of the invention is to provide a bactericide containing the bactericidal composition, wherein the mass of the active ingredients accounts for 1-65% of the total mass of the bactericide, and the balance is an agriculturally and pharmaceutically acceptable auxiliary agent.
Compared with the prior art, the invention has the following beneficial effects:
when 2 effective components in the bactericidal composition and the bactericide are compounded, the co-toxicity coefficient is more than 120 within a certain mass range, and the synergistic effect type is shown for preventing and treating the avocado canker. Compared with any single component contained in the pesticide composition, the pesticide composition can improve the control effect on the avocado canker, and on the basis, the application dosage of the active component can be reduced, the pesticide residue is reduced, and the environmental stress is relieved.
Detailed Description
The invention will be better understood from the following examples.
Examples: screening of prevention and treatment medicament for avocado canker
Test subjects: the bacterial strain of avocado canker (dothiella sp.) is obtained by collecting fruit infected by the bacterial strain of avocado canker, purifying by conventional tissue isolation method in laboratory, and storing on PDA culture medium.
Reagent to be tested: 99% of levocarvone raw drug (Shanghai Aladdin Biotechnology Co., Ltd.), 96% of diniconazole raw drug (Jiangsu Changlong agricultural chemical Co., Ltd.), 97% of tebuconazole raw drug (e.g. Dongzhong Ying chemical Co., Ltd.), and 96% of triazolone raw drug (Shanxi Lvhai pesticide technology Co., Ltd.).
The test method comprises the following steps: (refer to "NY/T1156.2-2006 indoor bioassay Standard for agricultural chemicals Fungicide part 2: inhibition of pathogenic fungi hypha growth test plate method")
1. Dissolving the raw medicine with dimethyl sulfoxide, diluting with 0.1% Tween-80 water solution to respectively prepare single-dose mother liquor, setting multiple groups of ratios, and setting 6 gradient mass concentrations for each single dose and each group of ratio mixture.
2. Adding 9mL of pre-melted PDA culture medium into a sterile conical flask, sequentially and quantitatively sucking 1mL of liquid medicine from low concentration and high concentration, respectively adding into the conical flasks, and sufficiently shaking. Then equivalently guiding into 3 culture dishes with the diameter of 9cm to prepare drug-containing plates with corresponding concentrations; treatments without agent were blanked, 3 replicates of each treatment.
3. And (3) taking a fungus cake with the diameter of 5mm from the propagated bacterial colony of the pear canker pathogen by using a puncher, inoculating the center of a drug-containing plate and the center of a blank control plate, and culturing in an illumination incubator with the relative humidity of 70% and the temperature of 28 ℃.
4. After culturing for 4 days, the colony diameter is measured by a cross method, and the inhibition rate of different treatments on the growth of hyphae is calculated. Using the logarithm value of the medicament as an independent variable and the value of the hypha growth inhibition rate as a dependent variable, and analyzing by using motion DPS software to obtain a toxicity regression equation and the toxicity EC of the medicament on the target bacteria 50 Values, and the co-toxicity coefficient (CTC) was calculated according to the grandsinope method.
D＝D 1 -D 2 Wherein D is the colony growing diameter; d 1 The diameter of the colony is shown; d 2 The diameter of the fungus cake;
I＝[(D 0 -D t )÷D0]x 100, wherein I is hyphal growth inhibition (%); d 0 Growing the diameter of the colonies for the blank control; d t Growing the diameter of the bacterial colony for medicament treatment;
measured virulence index (ATI) ═ standard agent EC 50 Reagent for test EC 50 )×100；
Theoretical virulence index (TTI) ═ a agent virulence index x percent of a in the mixture + B agent virulence index x percent of B in the mixture;
co-toxicity coefficient (CTC) × 100 [ actually measured toxicity index (ATI) ÷ theoretical toxicity index of the mixture (TTI) ].
The criteria are divided by joint action: the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80< co-toxicity coefficient (CTC) <120 showed additive effect. The results are shown in tables 1-3.
TABLE 1 indoor determination of biological activity of L-carvone and diniconazole combination on bacterial canker of avocado
Name and proportion of the medicament EC50(mg/L) ATI TTI CTC
L-carvone 3.91 100.00 -- --
Diniconazole 16.67 23.46 -- --
L-carvone 1: diniconazole 20 7.75 50.45 27.10 186.17
L-carvone 1: diniconazole 10 5.53 70.71 30.41 232.48
L-carvone 1: diniconazole 5 8.58 45.57 36.21 125.84
L-carvone 1: diniconazole 3 3.16 123.73 42.59 290.51
L-carvone 1: diniconazole 1 4.90 79.80 61.73 129.27
3, levo-carvone: diniconazole 1 2.62 149.24 80.86 184.55
L-carvone 5: diniconazole 1 1.43 273.43 87.24 313.41
L-carvone 7: diniconazole 1 3.41 114.66 90.43 126.79
L-carvone 12: diniconazole 1 2.59 150.97 94.11 160.41
As can be seen from Table 1, the mass ratio of the components is 1-12: within the range of 20-1, the co-toxicity coefficient of the compound of the levo-carvone and the diniconazole to the germs of the avocado canker is between 125.84 and 313.41, and is more than 120, which shows a synergistic effect.
TABLE 2 indoor determination of biological Activity of L-carvone and Tebuconazole combinations against the pathogen avocado canker
Name and proportion of the medicament EC50(mg/L) ATI TTI CTC
L-carvone 3.91 100.00 -- --
Tebuconazole 7.78 50.26 -- --
L-carvone 1: tebuconazole 30 6.11 63.99 51.86 123.39
L-carvone 1: tebuconazole 20 4.84 80.79 52.63 153.51
L-carvone 1: tebuconazole 10 3.55 110.14 54.78 201.06
L-carvone 1: tebuconazole 5 5.04 77.58 58.55 132.51
L-carvone 1: tebuconazole 3 2.91 134.36 62.69 214.32
L-carvone 1: tebuconazole 1 3.86 101.30 75.13 134.83
3, levo-carvone: tebuconazole 1 3.11 125.72 87.56 143.58
L-carvone 5: tebuconazole 1 2.10 186.19 91.71 203.02
L-carvone 10: tebuconazole 1 1.92 203.65 95.48 213.29
L-carvone 20: tebuconazole 1 1.12 349.11 97.63 357.58
Levo carvone 30: tebuconazole 1 0.93 420.43 98.40 427.29
L-carvone 40: tebuconazole 1 2.05 190.73 98.79 193.07
As can be seen from Table 2, the mass ratio of the components is 1 to 40: within the range of 30-1, the co-toxicity coefficient of the compound of the levo-carvone and the tebuconazole to the erwinia amylovora germs is between 123.39 and 427.29, and is all more than 120, and the synergistic effect is shown.
TABLE 3 indoor bioactivity assay of L-carvone and triazolone combination against bacterial canker of avocado
Name and proportion of the medicament EC50(mg/L) ATI TTI CTC
L-carvone 3.91 100.00 -- --
Triazolones 29.96 13.05 -- --
L-carvone 1: triazolones 15 16.43 23.80 18.49 128.74
L-carvone 1: triazolones 10 9.76 40.06 20.96 191.18
L-carvone 1: triazolones 5 8.42 46.44 27.54 168.60
L-carvone 1: triazolones 3 5.94 65.82 34.79 189.22
L-carvone 1: triazolone 1 4.51 86.70 56.53 153.38
3, levo-carvone: triazolone 1 2.25 173.78 78.26 222.04
Levo-carvone 5: triazolones 1 3.79 103.17 85.51 120.65
L-carvone 10: triazolones 1 2.44 160.25 92.10 174.00
L-carvone 20: triazolones 1 2.88 135.76 95.86 141.63
L-carvone 40: triazolones 1 1.36 287.50 97.88 293.73
L-carvone 60: triazolones 1 1.83 213.66 98.57 216.75
As can be seen from Table 3, the mass ratio of the components is 1 to 60: 15-1, the co-toxicity coefficient of the combination of the levo-carvone and the triazolone on the germs of the avocado canker is between 120.65 and 293.73, and is both more than 120, and the composition shows synergistic effect.
In conclusion, after the levo-carvone and the diniconazole, the tebuconazole or the triazolone are compounded, the compound has a good synergistic effect, the control effect of the avocado canker can be improved, the pesticide application dosage can be reduced on the basis, the control cost is reduced, the application dosage of active ingredients can be reduced on the basis, the pesticide residue is reduced, and the environmental pressure is relieved.
The foregoing is only a preferred embodiment of the present invention and modifications, which are within the scope of the invention, will be apparent to those skilled in the art without departing from the principles of the invention.

Claims (5)

1. The bactericidal composition for preventing and treating the avocado canker is characterized in that the effective component of the bactericidal composition is prepared by compounding levocarvone and diniconazole, tebuconazole or triazolone.
2. The bactericidal composition according to claim 1, wherein the mass ratio of the levocarvone to the diniconazole is 1-12: 20-1.
3. The bactericidal composition according to claim 1, wherein the mass ratio of the levocarvone to the tebuconazole is 1-40: 30-1.
4. The bactericidal composition according to claim 1, wherein the mass ratio of the levocarvone to the triazolone is 1-60: 15-1.
5. A bactericide comprising the bactericidal composition of any one of claims 1 to 4, wherein the mass of the active ingredient accounts for 1 to 65 percent of the total mass of the bactericide, and the balance is an agriculturally and pharmaceutically acceptable auxiliary agent.
CN202210829665.8A 2022-07-14 2022-07-14 Bactericide for preventing and treating avocado canker Pending CN115053900A (en)

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CN202210829665.8A CN115053900A (en) 2022-07-14 2022-07-14 Bactericide for preventing and treating avocado canker

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CN115053900A true CN115053900A (en) 2022-09-16

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