CN115044625A - Aloe-emodin refining method - Google Patents
Aloe-emodin refining method Download PDFInfo
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- CN115044625A CN115044625A CN202210890677.1A CN202210890677A CN115044625A CN 115044625 A CN115044625 A CN 115044625A CN 202210890677 A CN202210890677 A CN 202210890677A CN 115044625 A CN115044625 A CN 115044625A
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- YDQWDHRMZQUTBA-UHFFFAOYSA-N Aloe emodin Chemical compound C1=CC=C2C(=O)C3=CC(CO)=CC(O)=C3C(=O)C2=C1O YDQWDHRMZQUTBA-UHFFFAOYSA-N 0.000 title claims abstract description 144
- 238000000034 method Methods 0.000 title claims abstract description 40
- 238000007670 refining Methods 0.000 title claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 47
- 229940069521 aloe extract Drugs 0.000 claims abstract description 39
- 102000004190 Enzymes Human genes 0.000 claims abstract description 34
- 108090000790 Enzymes Proteins 0.000 claims abstract description 34
- 238000006243 chemical reaction Methods 0.000 claims abstract description 33
- 238000009835 boiling Methods 0.000 claims abstract description 18
- 238000010438 heat treatment Methods 0.000 claims abstract description 17
- 239000007864 aqueous solution Substances 0.000 claims description 49
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 46
- 239000000243 solution Substances 0.000 claims description 45
- 239000008096 xylene Substances 0.000 claims description 42
- 239000012065 filter cake Substances 0.000 claims description 30
- 239000012043 crude product Substances 0.000 claims description 25
- 238000001914 filtration Methods 0.000 claims description 13
- 239000000047 product Substances 0.000 claims description 10
- 239000013078 crystal Substances 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 8
- 238000000746 purification Methods 0.000 claims description 6
- 238000003825 pressing Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 239000002994 raw material Substances 0.000 abstract description 7
- 239000007788 liquid Substances 0.000 abstract description 3
- 230000002035 prolonged effect Effects 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 2
- 230000035484 reaction time Effects 0.000 abstract 1
- 239000012535 impurity Substances 0.000 description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000011085 pressure filtration Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 238000002425 crystallisation Methods 0.000 description 3
- 230000008025 crystallization Effects 0.000 description 3
- 241001116389 Aloe Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000007792 addition Methods 0.000 description 2
- 235000011399 aloe vera Nutrition 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000004366 Glucosidases Human genes 0.000 description 1
- 108010056771 Glucosidases Proteins 0.000 description 1
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 description 1
- -1 anthraquinone compound Chemical class 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/66—Preparation of oxygen-containing organic compounds containing the quinoid structure
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C46/00—Preparation of quinones
- C07C46/10—Separation; Purification; Stabilisation; Use of additives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C2603/00—Systems containing at least three condensed rings
- C07C2603/02—Ortho- or ortho- and peri-condensed systems
- C07C2603/04—Ortho- or ortho- and peri-condensed systems containing three rings
- C07C2603/22—Ortho- or ortho- and peri-condensed systems containing three rings containing only six-membered rings
- C07C2603/24—Anthracenes; Hydrogenated anthracenes
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to the technical field of refining of aloe-emodin, in particular to a method for refining aloe-emodin. The method comprises the following steps: heating the water solution containing Aloe extract to boiling, reducing the temperature of the water solution to enzymolysis temperature of reaction enzyme, and adding reaction enzyme into the water solution for enzymolysis to obtain Aloe-emodin. In the prior art, although the aloe-emodin can be obtained by adding the reaction enzyme into the aloe extract solution for enzymolysis, the enzymolysis reaction time is short, which easily causes insufficient enzymolysis process and waste of raw materials. Compared with the prior art, the invention provides a reasonable liquid environment for the reaction enzyme in advance, so that the enzyme activity can be maintained for a longer time, the enzymolysis time is further effectively prolonged, the enzymolysis process is more sufficient, and the raw materials are more fully utilized.
Description
Technical Field
The invention relates to the technical field of refining of aloe-emodin, in particular to a method for refining aloe-emodin.
Background
The aloe-emodin is anthraquinone compound, and has antibacterial, anticancer, and hair texture improving effects. Among them, the aloe-emodin has a special anticancer effect, so that scholars at home and abroad are interested in the research of the aloe-emodin. Aloe-emodin is obtained from many sources, and one of the sources is aloe. As an economic crop, how to efficiently obtain aloe-emodin from aloe becomes one of important links for controlling the cost of the aloe-emodin.
Chinese patent discloses a refining and purifying method of aloe-emodin [ application number: CN201811422356.9, publication No.: CN109517855A ] includes: the method comprises the following steps: step 1, taking aloe extract as a raw material, and performing enzymolysis step by step to obtain an aloe-emodin crude product; dissolving Aloe extract in 60% ethanol in a glass reaction kettle under heating and stirring, standing the solution, filtering to remove residue, and collecting supernatant; placing the supernatant into a reaction kettle, wherein the addition amount of the recombinant glucosidase is 60U/mL, the enzymolysis temperature is about 65 ℃, the enzymolysis pH is about 5.0, and the enzymolysis time is about 3 hours; then cooling the solution after enzymolysis, standing for crystallization for 24h to obtain aloe-emodin crude product, concentrating the mother liquor, cooling for crystallization to obtain precipitate, repeating twice, wherein the mother liquor is the solution after enzymolysis; step 2, purifying the aloe-emodin crude product to obtain an aloe-emodin refined product; recrystallizing the aloe-emodin crude product with toluene 10 times the weight of the aloe-emodin crude product at 40-50 deg.C, adding 10% active carbon after completely dissolving, and repeating the crystallization twice; drying the crystallized precipitate, washing with 2-3 times of methanol, centrifuging, drying to obtain refined aloe-emodin, mixing the washing solutions, concentrating, crystallizing, and recovering methanol. Although the technical scheme of the patent provides an effective method for obtaining aloe-emodin by enzymolysis, the technical scheme of the patent is limited by the obtaining process, the enzymolysis time is short and is only 3 hours, so that the enzymolysis process is insufficient, and raw materials are easily wasted.
Disclosure of Invention
Aiming at the technical problems in the prior art, the invention provides a method for refining aloe-emodin.
In order to solve the technical problems, the invention provides the following technical scheme:
a method for refining aloe-emodin comprises: heating the water solution containing Aloe extract to boiling, reducing the temperature of the water solution to enzymolysis temperature of reaction enzyme, and adding reaction enzyme into the water solution for enzymolysis to obtain Aloe-emodin.
In the actual state, the aloe extract is dissolved by water. After dissolution, the aqueous solution is heated to boiling. Therefore, impurities which are not high temperature resistant in the aqueous solution and impurities such as bacteria, fungi and the like can be removed, so that the impurities in the aqueous solution can be prevented from influencing the activity of the reaction enzyme. When the aqueous solution is boiled, the temperature of the aqueous solution is lowered to the enzymolysis temperature of the reaction enzyme. At this time, a reaction enzyme is added to the aqueous solution for enzymatic hydrolysis to obtain aloe-emodin. In conclusion, the invention provides a reasonable liquid environment for the reaction enzyme in advance, effectively reduces impurities which may influence the enzyme activity in the aqueous solution, so that the activity of the reaction enzyme can be maintained for a longer time, further effectively prolongs the enzymolysis time, makes the enzymolysis process more sufficient, and makes the utilization of raw materials more sufficient.
Further, the method also comprises the following steps:
a dissolving step: injecting water into the aloe extract to obtain an aqueous solution, heating the aqueous solution to boiling, reducing the temperature of the aqueous solution, and filtering the aqueous solution when the temperature of the aqueous solution is reduced to the enzymolysis temperature of the reaction enzyme;
an enzymolysis step: adding reaction enzyme into the water solution for enzymolysis to obtain aloe-emodin crude product;
a purification step: dissolving the aloe-emodin crude product into xylene to obtain a xylene solution, reducing the temperature of the xylene solution to crystallize the aloe-emodin, and filtering out the crystals to obtain aloe-emodin fine product.
Further, the dissolving step further comprises the steps of:
crushing the aloe extract until the fragment of the aloe extract is less than 5 cm;
dissolving the crushed aloe extract in water to obtain an aqueous solution;
heating the aqueous solution to boil, and maintaining the boiling for a specified time;
reducing the temperature of the aqueous solution to a specified temperature;
when the temperature of the aqueous solution was lowered to the specified temperature, the aqueous solution was filtered.
Furthermore, the mass ratio of the water to the aloe extract is more than 15 and less than 20.
Further, the specified time period is more than 0.5h and less than 1 h.
Further, temperatures greater than 40 ℃ and less than 50 ℃ are specified.
Further, the enzymolysis time of the reaction enzyme is more than 70 hours and less than 75 hours.
Further, the purification step further comprises the steps of:
filter-pressing the aloe-emodin crude product to obtain a crude product filter cake;
crushing the crude filter cake;
heating xylene, and dissolving the crushed crude product filter cake into the xylene to obtain a xylene solution;
reducing the temperature of the xylene solution to crystallize aloe-emodin
Filtering out crystals to obtain a fine filter cake;
drying the refined filter cake to obtain the aloe-emodin refined product.
Further, the heating temperature of the dimethylbenzene is more than 60 ℃ and less than 70 ℃.
Compared with the prior art, the invention has the following advantages:
by utilizing the dissolving step, insoluble impurities, impurities which are not high temperature resistant, and biological impurities such as bacteria, fungi and the like are effectively removed. The influence of the impurities on the pH value in the enzymolysis process is effectively avoided, so that the activity of the reaction enzyme can be maintained for a longer time, the enzymolysis process is effectively prolonged, and raw materials are fully utilized.
The invention can effectively utilize the water resource for dissolving the aloe extract and effectively reduce the waste of the water resource.
The dissolving step is utilized to ensure that the activity of the reaction enzyme can be maintained for a longer time, thereby facilitating the subsequent recovery and reuse of the reaction enzyme.
Drawings
FIG. 1: the overall flow chart.
Detailed Description
The following are specific embodiments of the present invention and are further described with reference to the drawings, but the present invention is not limited to these embodiments.
The first embodiment is as follows:
a method for refining aloe-emodin comprises: heating the water solution containing Aloe extract to boiling, reducing the temperature of the water solution to enzymolysis temperature of reaction enzyme, and adding reaction enzyme into the water solution for enzymolysis to obtain Aloe-emodin.
Specifically, the method comprises the following steps: solution step, enzymolysis step and purification step.
Wherein the dissolving step comprises the following steps: crushing the aloe extract until the fragment of the aloe extract is less than 5 cm. Injecting water into the crushed aloe extract to dissolve the aloe extract into the water to obtain an aqueous solution. Wherein the mass ratio of the water to the aloe extract is more than 15 and less than 20. Heating the aqueous solution to boiling, and maintaining the boiling for a specified time, wherein the specified time is more than 0.5h and less than 1 h. And when the boiling time reaches the specified time, reducing the temperature of the aqueous solution to the specified temperature. Temperatures greater than 40 ℃ and less than 50 ℃ are specified. After the temperature of the aqueous solution was reduced to the specified temperature, the aqueous solution was filtered to remove insoluble solid impurities from the aqueous solution.
The enzymolysis step specifically comprises the following processes: adding reaction enzyme into the water solution for enzymolysis to obtain aloe-emodin crude product. The enzymolysis time is more than 70h and less than 75 h.
The purification step specifically comprises the following steps: and (3) carrying out pressure filtration on the aloe-emodin crude product to obtain a crude product filter cake. And crushing the crude filter cake. The xylene is heated such that the temperature of the xylene rises above 60 ℃ to less than 70 ℃. And dissolving the crushed crude filter cake into xylene to obtain a xylene solution. The temperature of the xylene solution is lowered to cause the internal aloe-emodin to precipitate as aloe-emodin crystals. The crystals are filtered off to obtain a fine filter cake. Drying the refined filter cake to obtain the aloe-emodin refined product.
In the actual state. Crushing the aloe extract until the length of the broken blocks of the aloe extract is 5 cm. Injecting water into the crushed aloe extract to dissolve the aloe extract into the water to obtain an aqueous solution. Wherein the mass ratio of the water to the aloe extract is 15. Heating the aqueous solution to boil, and maintaining the boiling state for 0.5 h. After boiling for 0.5h, the temperature of the aqueous solution was reduced to 40 ℃. After the aqueous solution temperature was reduced to 40 ℃, the aqueous solution was filtered to remove solid impurities that were insoluble in the aqueous solution. Therefore, through the process, a reasonable liquid environment is provided for the subsequent enzymolysis process on the one hand. On the other hand, insoluble impurities which are not high temperature resistant in the water solution are effectively removed. Meanwhile, biological impurities such as bacteria and fungi mixed in the water solution are killed. So as to avoid the uncontrollable influence of the impurities on the pH value of the aqueous solution along with the increase of the enzymolysis time in the subsequent enzymolysis process, and further influence on the activity of the reaction enzyme. Therefore, the activity of the reaction enzyme can be maintained for a longer time by utilizing the process, so that the enzymolysis time is effectively prolonged, and the raw materials are more fully utilized. After the aqueous solution is filtered, adding a reaction enzyme into the aqueous solution for enzymolysis to obtain a crude aloe-emodin product. Wherein the enzymolysis time is 70 h. By utilizing the dissolving step, the activity of the reaction enzyme is well maintained, so that the reaction enzyme is conveniently recycled after the enzymolysis process. Meanwhile, water used in the dissolving step serves as a dissolving agent and an environmental carrier in the enzymolysis process. Therefore, water resources are fully utilized. And after the enzymolysis process is finished, carrying out pressure filtration on the aloe-emodin crude product to obtain a crude product filter cake. And crushing the crude filter cake. The xylene was heated so that the temperature of the xylene was raised to 60 ℃. At this time, the solubility of xylene to aloe-emodin was higher. And dissolving the crushed crude filter cake into xylene to obtain a xylene solution. The xylene solution was filtered to remove insoluble impurities. The temperature of the xylene solution is reduced to reduce the solubility of the xylene to aloe-emodin, such that the aloe-emodin crystallizes in the xylene. Filtering out the crystals to obtain a refined filter cake, and drying the refined filter cake to obtain the aloe-emodin refined product.
Example two:
in the actual state. Crushing the aloe extract until the length of the broken blocks of the aloe extract is 3 cm. Injecting water into the crushed aloe extract to dissolve the aloe extract into the water to obtain an aqueous solution. Wherein the mass ratio of the water to the aloe extract is 18. Heating the aqueous solution to boil, and maintaining the boiling state for 0.7 h. After boiling for 0.7h, the temperature of the aqueous solution was reduced to 45 ℃. When the temperature of the water solution is reduced to 45 ℃, filtering the water solution, and after the water solution is filtered, adding reactive enzyme into the water solution for enzymolysis to obtain the aloe-emodin crude product. Wherein the enzymolysis time is 73 h. And after the enzymolysis process is finished, carrying out pressure filtration on the aloe-emodin crude product to obtain a crude product filter cake. And crushing the crude filter cake. The xylene was heated such that the temperature of the xylene was increased to 65 ℃. And dissolving the crushed crude filter cake into xylene to obtain a xylene solution. The xylene solution was filtered to remove insoluble impurities. The temperature of the xylene solution is reduced, causing aloe-emodin to crystallize in the xylene. Filtering out the crystals to obtain a refined filter cake, and drying the refined filter cake to obtain the aloe-emodin refined product.
Example three:
in the actual state. Crushing the aloe extract until the length of the broken blocks of the aloe extract is 2 cm. Injecting water into the crushed aloe extract to dissolve the aloe extract into the water to obtain an aqueous solution. Wherein the mass ratio of the water to the aloe extract is 20. Heating the aqueous solution to boil, and maintaining the boiling state for 1 h. After boiling for 1h, the temperature of the aqueous solution was reduced to 50 ℃. When the temperature of the water solution is reduced to 50 ℃, filtering the water solution, and after filtering the water solution, adding a reaction enzyme into the water solution for enzymolysis to obtain the aloe-emodin crude product. Wherein the enzymolysis time is 75 h. And after the enzymolysis process is finished, carrying out pressure filtration on the aloe-emodin crude product to obtain a crude product filter cake. And crushing the crude filter cake. The xylene was heated so that the temperature of the xylene was raised to 70 ℃. And dissolving the crushed crude filter cake into xylene to obtain a xylene solution. The xylene solution was filtered to remove insoluble impurities. The temperature of the xylene solution is reduced, allowing the aloe-emodin to crystallize in xylene. Filtering out the crystals to obtain a refined filter cake, and drying the refined filter cake to obtain the aloe-emodin refined product.
The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the invention as defined in the appended claims.
Claims (9)
1. A method for refining aloe-emodin is characterized by comprising the following steps: the method comprises the following steps: heating the water solution with the aloe extract to boil, reducing the temperature of the water solution to the enzymolysis temperature of the reaction enzyme, and adding the reaction enzyme into the water solution for enzymolysis to obtain the aloe-emodin.
2. The method for refining aloe-emodin according to claim 1, characterized in that: further comprising the steps of:
a dissolving step: injecting water into the aloe extract to obtain the aqueous solution, heating the aqueous solution to boiling, reducing the temperature of the aqueous solution, and filtering the aqueous solution when the temperature of the aqueous solution is reduced to the enzymolysis temperature of the reaction enzyme;
an enzymolysis step: adding the reaction enzyme into the aqueous solution for enzymolysis to obtain aloe-emodin crude product; a purification step: dissolving the aloe-emodin crude product into xylene to obtain a xylene solution, reducing the temperature of the xylene solution to crystallize the aloe-emodin, and filtering the crystals to obtain aloe-emodin fine product.
3. The method for refining aloe-emodin according to claim 2, characterized in that: the dissolving step further comprises the steps of:
crushing the aloe extract until the broken blocks of the aloe extract are less than 5 cm;
dissolving the crushed aloe extract in water to obtain the aqueous solution;
heating the aqueous solution to boiling, and maintaining boiling for a specified time;
reducing the temperature of the aqueous solution to a specified temperature;
when the temperature of the aqueous solution is lowered to a specified temperature, the aqueous solution is filtered.
4. The method for refining aloe-emodin according to claim 3, characterized in that: the mass ratio of the water to the aloe extract is more than 15 and less than 20.
5. The method for refining aloe-emodin according to claim 3, characterized in that: the specified time length is more than 0.5h and less than 1 h.
6. The method for refining aloe-emodin according to claim 3, characterized in that: the specified temperature is greater than 40 ℃ and less than 50 ℃.
7. The method for refining aloe-emodin according to claim 2, characterized in that: the enzymolysis time of the reaction enzyme is more than 70h and less than 75 h.
8. The method for refining aloe-emodin according to claim 2, characterized in that: the purification step further comprises the steps of:
filter-pressing the aloe-emodin crude product to obtain a crude product filter cake;
pulverizing the crude filter cake;
heating the xylene, and dissolving the crushed crude filter cake into the xylene to obtain a xylene solution;
reducing the temperature of the xylene solution to crystallize the aloe-emodin
Filtering out the crystals to obtain a fine filter cake;
drying the refined filter cake to obtain the aloe-emodin refined product.
9. The method for refining aloe-emodin according to claim 1, characterized in that: the heating temperature of the dimethylbenzene is more than 60 ℃ and less than 70 ℃.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006051400A1 (en) * | 2004-11-12 | 2006-05-18 | Laboratoire Medidom S.A. | Process for preparing aloe-emodin |
| CN102080108A (en) * | 2010-12-15 | 2011-06-01 | 云南弗蓝替生物工程有限公司 | Method for extracting emodin, polydatin and resveratrol from polygonum cuspidatum by using vacuum enzymolysis technology |
| CN102220387A (en) * | 2011-04-12 | 2011-10-19 | 安康中科麦迪森天然药业有限公司 | Method for extracting and purifying resveratrol and emodin from fresh giant knotweed |
| CN109517855A (en) * | 2018-11-27 | 2019-03-26 | 西安天丰生物科技有限公司 | A kind of polishing purification method of aloe-emodin |
-
2022
- 2022-07-27 CN CN202210890677.1A patent/CN115044625A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006051400A1 (en) * | 2004-11-12 | 2006-05-18 | Laboratoire Medidom S.A. | Process for preparing aloe-emodin |
| CN102080108A (en) * | 2010-12-15 | 2011-06-01 | 云南弗蓝替生物工程有限公司 | Method for extracting emodin, polydatin and resveratrol from polygonum cuspidatum by using vacuum enzymolysis technology |
| CN102220387A (en) * | 2011-04-12 | 2011-10-19 | 安康中科麦迪森天然药业有限公司 | Method for extracting and purifying resveratrol and emodin from fresh giant knotweed |
| CN109517855A (en) * | 2018-11-27 | 2019-03-26 | 西安天丰生物科技有限公司 | A kind of polishing purification method of aloe-emodin |
Non-Patent Citations (2)
| Title |
|---|
| 钟桂芳等: "基于响应曲面法的芦荟大黄素制备工艺研究", 河南农业大学学报, vol. 49, no. 5, 31 October 2015 (2015-10-31), pages 701 - 706 * |
| 钟桂芳等: "生物法制备芦荟大黄素的研究", 郑州轻工业学院学报(自然科学版), vol. 30, no. 2, 30 April 2015 (2015-04-30), pages 26 - 29 * |
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