CN115029335B - 一种耐高温木聚糖酶突变体及其应用 - Google Patents
一种耐高温木聚糖酶突变体及其应用 Download PDFInfo
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- CN115029335B CN115029335B CN202210814339.XA CN202210814339A CN115029335B CN 115029335 B CN115029335 B CN 115029335B CN 202210814339 A CN202210814339 A CN 202210814339A CN 115029335 B CN115029335 B CN 115029335B
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- xylanase
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- enzyme activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
- C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/885—Trichoderma
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
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- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
木聚糖酶突变体 | 95℃处理5min后酶活残留率 |
PT | 70.00% |
T23S | 81.30% |
S39D | 80.18% |
T41N | 79.77% |
S62Q | 81.70% |
L64M | 87.90% |
W116V | 79.45% |
A139G | 81.00% |
T149F | 82.00% |
N160E | 86.00% |
N160V | 76.99% |
Y144F | 84.79% |
Claims (7)
Priority Applications (3)
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CN202311344298.3A CN117384889A (zh) | 2022-07-12 | 2022-07-12 | 耐高温木聚糖酶突变体及其应用 |
CN202210814339.XA CN115029335B (zh) | 2022-07-12 | 2022-07-12 | 一种耐高温木聚糖酶突变体及其应用 |
CN202311344279.0A CN117384888A (zh) | 2022-07-12 | 2022-07-12 | 一种耐高温木聚糖酶突变体及其应用 |
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CN202210814339.XA CN115029335B (zh) | 2022-07-12 | 2022-07-12 | 一种耐高温木聚糖酶突变体及其应用 |
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CN202311344279.0A Division CN117384888A (zh) | 2022-07-12 | 2022-07-12 | 一种耐高温木聚糖酶突变体及其应用 |
CN202311344298.3A Division CN117384889A (zh) | 2022-07-12 | 2022-07-12 | 耐高温木聚糖酶突变体及其应用 |
Publications (2)
Publication Number | Publication Date |
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CN115029335A CN115029335A (zh) | 2022-09-09 |
CN115029335B true CN115029335B (zh) | 2024-04-16 |
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CN202210814339.XA Active CN115029335B (zh) | 2022-07-12 | 2022-07-12 | 一种耐高温木聚糖酶突变体及其应用 |
CN202311344298.3A Pending CN117384889A (zh) | 2022-07-12 | 2022-07-12 | 耐高温木聚糖酶突变体及其应用 |
CN202311344279.0A Pending CN117384888A (zh) | 2022-07-12 | 2022-07-12 | 一种耐高温木聚糖酶突变体及其应用 |
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CN202311344298.3A Pending CN117384889A (zh) | 2022-07-12 | 2022-07-12 | 耐高温木聚糖酶突变体及其应用 |
CN202311344279.0A Pending CN117384888A (zh) | 2022-07-12 | 2022-07-12 | 一种耐高温木聚糖酶突变体及其应用 |
Country Status (1)
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