CN115028865A - Mango peel and fruit gum based photodynamic antibacterial film and preparation method and application thereof - Google Patents

Mango peel and fruit gum based photodynamic antibacterial film and preparation method and application thereof Download PDF

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CN115028865A
CN115028865A CN202210692852.6A CN202210692852A CN115028865A CN 115028865 A CN115028865 A CN 115028865A CN 202210692852 A CN202210692852 A CN 202210692852A CN 115028865 A CN115028865 A CN 115028865A
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mango peel
mango
film
pectin
antibacterial film
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CN115028865B (en
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邹颖
周伟
彭芍丹
何云侠
李如一
林燕云
李积华
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Agricultural Products Processing Research Institute of CATAS
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
    • C08J5/18—Manufacture of films or sheets
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00—Preservation or chemical ripening of fruit or vegetables
    • A23B7/015—Preserving by irradiation or electric treatment without heating effect
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00—Preservation or chemical ripening of fruit or vegetables
    • A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/154—Organic compounds; Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
    • C08J2305/06—Pectin; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
    • C08J2405/04—Alginic acid; Derivatives thereof
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    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K5/00—Use of organic ingredients
    • C08K5/04—Oxygen-containing compounds
    • C08K5/13—Phenols; Phenolates
    • C08K5/132—Phenols containing keto groups, e.g. benzophenones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/85—Food storage or conservation, e.g. cooling or drying

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Abstract

The invention discloses a mango peel and fruit gum base photodynamic antibacterial film and a preparation method and application thereof, wherein the preparation method of the antibacterial film comprises the following steps: (1) blanching mango peel for inactivating enzyme, and freeze-drying to obtain mango peel powder; (2) mixing mango peel powder with a citric acid solution to obtain a mixed solution, adjusting the pH value, performing ultrasonic extraction, and performing ultrahigh pressure treatment to obtain a mango extracting solution; (3) performing solid-liquid separation on the mango extracting solution, taking a precipitate, and performing freeze drying to obtain mango peel pectin; (4) carrying out water bath on a mixed solution of mango peel pectin liquid and sodium alginate solution, adding glycerol, uniformly mixing, adding curcumin, and carrying out high-speed shearing and ultrasonic crushing to obtain a film forming liquid; (5) and (3) after the film forming solution is subjected to vacuum deaeration, pouring the film by adopting a casting method, drying, taking out, and uncovering the film to obtain the film. The photodynamic antibacterial film reduces the juice loss and the microbial quantity of the fresh-cut fruits in the storage process, and has good fresh-keeping effect.

Description

Mango peel and fruit gum based photodynamic antibacterial film and preparation method and application thereof
Technical Field
The invention relates to the field of non-thermal sterilization, in particular to a mango peel and fruit gum base photodynamic antibacterial film and a preparation method and application thereof.
Background
Fresh-cut fruit is a ready-to-eat or ready-to-use fruit product which is prepared by taking fresh fruits as raw materials, carrying out a series of treatments such as grading, cleaning, trimming, peeling, cutting, fresh keeping, packaging and the like, and then carrying out low-temperature transportation to enter a refrigerator for sale. The fresh-cut fruits keep the original fresh state of the fruits, and the products are clean and sanitary after processing, natural, nutritional, fresh and convenient, have high availability after being eaten by 100 percent, and can meet the requirements of people on aspects of pursuing natural, nutritional and fast-paced life styles and the like. However, mechanical damage to freshly cut fruits due to the cutting process can cause a series of physiological and biochemical changes, such as discoloration, off-taste, aging, softening, and deterioration due to microbial infestation, with a dramatic decrease in shelf life. Therefore, the key of the fresh-cut fruit processing technology is to maintain the quality and prolong the preservation period.
Currently, Photodynamic non-thermal sterilization technology (PNST), which is a non-thermal sterilization technology, is a method for generating active oxygen species by the action of a photosensitizer and a light source to achieve a sterilization effect. PNST preferentially acts on target cells of microorganisms to damage the target cells, influences normal physiological metabolic functions of the target cells, and does not damage surrounding cells and tissues. At present, the method is widely applied to the treatment of various diseases such as malignant tumors and the like in the medical field. The photodynamic non-thermal sterilization technology has the characteristics of high efficiency and broad-spectrum antibiosis, but the research of preparing the photodynamic sterilization preservative film based on the technology has few related reports at present, and if the photodynamic sterilization preservative film can be applied to fresh-cut fruits, the photodynamic sterilization preservative film has great potential.
Disclosure of Invention
In order to overcome the defects of the prior art, one of the purposes of the invention is to provide a preparation method of a mango peel and fruit gum based photodynamic antibacterial film.
The second purpose of the invention is to provide the mango peel and fruit gum base photodynamic antibacterial film prepared by the first purpose of the invention.
The third purpose of the invention is to provide an application method of the mango peel and fruit gum base photodynamic antibacterial film obtained by the second purpose in fresh keeping of fresh-cut fruits.
One of the purposes of the invention is realized by adopting the following technical scheme: a preparation method of a mango peel and fruit gum base photodynamic antibacterial film applied to fresh keeping of fresh-cut fruits comprises the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder;
(2) mixing the mango peel powder prepared in the step (1) with a citric acid solution to obtain a mixed solution, adjusting the pH value of the mixed solution, performing ultrasonic extraction, and performing ultrahigh pressure treatment to obtain a mango extracting solution;
(3) carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) mixing the mango peel pectin solution obtained in the step (3) with sodium alginate, carrying out water bath on the obtained mixed solution, fully dissolving and mixing, adding plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in an oil phase, carrying out high-speed shearing, and carrying out ultrasonic crushing treatment by an ultrasonic crusher to obtain a film forming solution;
(5) and (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring a film by adopting a tape casting method, drying, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film.
Further, in the step (1), the temperature of the blanching enzyme deactivation is 85-95 ℃, and the time of the blanching enzyme deactivation is 2-5 min.
Further, in the step (2), the material-liquid ratio of the mango peel powder to the citric acid solution is 1 (10-20); the pH value of the mixed solution is adjusted to 2.0-3.0.
Further, in the step (2), the extraction conditions of the ultrasound are as follows: the ultrasonic power is 60-100W, and the ultrasonic time is 15-25 min; the equipment adopted by the high-pressure treatment is ultrahigh-pressure sterilization equipment, and the high-pressure treatment condition is that the high-pressure treatment is carried out for 2-5min at 550 MPa.
Further, in the step (4), dissolving the mango peel pectin obtained in the step (3) and sodium alginate in water to obtain a mixed solution of 1-1.5% of mango peel pectin and 0.8-1.25% of sodium alginate by mass fraction.
Further, in the step (4), the photosensitizer curcumin dissolved in the oil phase is prepared by the following method: the oil phase adopted is medium chain triglyceride MCT; the content of the photosensitizer curcumin is 25-35 mg/ml.
Further, in the step (4), the water bath conditions are as follows: the water bath temperature is 65-75 deg.C, and the water bath time is 25-35 min; the mass fraction of the plasticizer glycerol is 1-1.2%; the high-speed shearing time is 2 min; the crushing treatment time is 2 min.
Further, in the step (5), the drying temperature is 25-35 ℃, and the drying time is more than 12 h.
The second purpose of the invention is realized by adopting the following technical scheme: the mango peel and fruit gum base photodynamic antibacterial film is prepared by the preparation method of the mango peel and fruit gum base photodynamic antibacterial film.
The third purpose of the invention is realized by adopting the following technical scheme: the method for applying the mango peel and fruit gum base photodynamic antibacterial film to fresh-cut fruits comprises the following steps: after fresh-cut fruits are packaged by the mango peel and fruit gum base photodynamic antibacterial film, LED visible light is adopted for irradiation; the LED visible light is from an LED visible light source with the wavelength of 405-420nm, the output light power is more than 280mW, and the irradiation time is 10-40 min.
Compared with the prior art, the invention has the beneficial effects that:
the mango peel pectin is extracted by using an ultrasonic and ultrahigh pressure assisted acid hydrolysis technology, the original pigment of the mango peel is reserved, the gelling property and the emulsifying property of the mango peel are improved, the photosensitizer curcumin is coated in the composite film to form a stable emulsion matrix, the solubility, the stability and the slow release property of the photosensitizer are improved to a certain degree through an emulsion system, the degradation of the photosensitizer under the non-LED irradiation condition is avoided, the composition of a film material is stabilized, the activity of an antibacterial substance is ensured, the water vapor permeability of a preservative film is reduced to a certain degree, and the juice loss and the microbial quantity of fresh-cut fruits in the storage process are reduced.
The mango peel pectin-curcumin-based photosensitive composite preservative film disclosed by the invention is simple and rapid in preparation method, is free from any toxic substance, is green and environment-friendly, improves the comprehensive utilization rate of mango peel, can save the preparation cost to a certain extent, is prepared at a lower temperature, does not need high temperature, saves energy, and more importantly ensures the biological activity of an active packaging film, and is especially applied to water loss of fresh-cut fruits.
Detailed Description
The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment.
In the present invention, all parts and percentages are by weight, unless otherwise specified, and the equipment and materials used are commercially available or commonly used in the art. The methods in the following examples are conventional in the art unless otherwise specified.
The invention provides a preparation method of a mango peel and fruit gum base photodynamic antibacterial film, which comprises the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder;
(2) mixing the mango peel powder prepared in the step (1) with a citric acid solution to obtain a mixed solution, adjusting the pH value of the mixed solution, performing ultrasonic extraction, and performing ultrahigh pressure treatment to obtain a mango extracting solution;
(3) carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) mixing the mango peel pectin solution obtained in the step (3) with sodium alginate, carrying out water bath on the obtained mixed solution, fully dissolving and mixing, adding plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in an oil phase, carrying out high-speed shearing, and carrying out ultrasonic crushing treatment by an ultrasonic crusher to obtain a film forming solution;
(5) and (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring a film by adopting a tape casting method, drying, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film.
The mango peel pectin prepared by the steps (1) - (3) has good film forming property and emulsifying property, and the reserved mango peel pigment and the photosensitizer curcumin are compounded to prepare an emulsion-based film forming solution, so that the solubility and the photodynamic efficiency of the curcumin are improved, the targeted aggregation release of the photosensitizer and the inactivation efficiency of food-borne pathogenic bacteria are enhanced, and the mango peel pectin is particularly suitable for being applied to fresh-keeping of fresh-cut fruits.
As a further preferable scheme, in the step (1), the temperature of the blanching enzyme deactivation is 85-95 ℃, and the time of blanching enzyme deactivation is 2-5 min.
As a further preferable scheme, in the step (2), the feed-to-liquid ratio of the mango peel powder to the citric acid solution is 1 (10-20); the pH value of the mixed solution is adjusted to 2.0-3.0.
As a further preferable scheme, in the step (2), the extraction conditions of the ultrasound are as follows: the ultrasonic power is 60-100W, and the ultrasonic time is 15-25 min; the equipment adopted by the high-pressure treatment is ultrahigh-pressure sterilization equipment, and the high-pressure treatment condition is that the high-pressure treatment is carried out for 2-5min at 550 MPa.
In a further preferable mode, in the step (4), the mango peel pectin obtained in the step (3) and sodium alginate are dissolved in water to obtain a mixed solution of the mango peel pectin with the mass fraction of 1-1.5% and the sodium alginate with the mass fraction of 0.8-1.25%.
As a further preferable mode, in the step (4), the photosensitizer curcumin dissolved in the oil phase is prepared by the following method: the oil phase is medium chain triglyceride MCT; the content of the photosensitizer curcumin is 25-35 mg/ml.
As a further preferable mode, in the step (4), the water bath conditions are as follows: the water bath temperature is 65-75 deg.C, and the water bath time is 25-35 min; the mass fraction of the plasticizer glycerol is 1-1.2%; the high-speed shearing time is 2 min; the crushing treatment time is 2 min.
As a further preferable scheme, in the step (5), the drying temperature is 25-35 ℃, and the drying time is more than 12 h.
The invention also provides a mango peel and fruit gum base photodynamic antibacterial film which is prepared by the preparation method of the mango peel and fruit gum base photodynamic antibacterial film.
The invention also provides an application of the mango peel and fruit gum base photodynamic antibacterial film, and the mango peel and fruit gum base photodynamic antibacterial film is applied to the fresh keeping of fresh-cut fruits.
The following are specific examples of the present invention, and raw materials, equipments and the like used in the following examples can be obtained by purchasing them unless otherwise specified.
Examples 1-6 and comparative examples 1-8:
[ example 1 ]
The mango peel pectin-based photodynamic antibacterial film of example 1 is prepared by the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with citric acid to obtain a mixed solution, adjusting the pH value of the mixed solution to 2.5, performing ultrasonic extraction, performing high-pressure treatment by adopting ultrahigh-pressure sterilization equipment, and performing ultrahigh-pressure 550MPa treatment for 3min to obtain a mango extracting solution; the feed-liquid ratio of the mango peel powder to the citric acid solution is 1: 15. The ultrasonic extraction conditions were as follows: the ultrasonic power is 80W, and the ultrasonic time is 20 min.
(3) Carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath on a mixed solution of mango peel pectin liquid with the mass fraction of 1.5% and sodium alginate solution with the mass fraction of 1.0% at 70 ℃ for 30min, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in chain triglyceride (MCT) in an oil phase, carrying out high-speed shearing for 2min, and carrying out ultrasonic crushing treatment for 2min by an ultrasonic crusher to obtain a film forming liquid; wherein the concentration of curcumin is 30 mg/mL.
(5) And (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring the film by adopting a tape casting method, drying at 25-35 ℃ for 12h, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (3) after the fresh-cut pineapples are packaged by the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5), irradiating by using LED visible light to realize an antibacterial effect, wherein the LED visible light is from an LED visible light source with the wavelength of 405 and 420nm, the output light power is more than 280mW, and the irradiation time by using the LED visible light is 10 min.
[ example 2 ] A method for producing a polycarbonate
The difference between the embodiment 2 and the embodiment 1 is that the illumination time of the LED is different, the illumination time of the embodiment 2 is 20min, and the rest conditions are the same.
The mango peel pectin-based photodynamic antibacterial film of example 2 is prepared by the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with a citric acid solution to obtain a mixed solution, adjusting the pH value of the mixed solution to 2.5, performing ultrasonic extraction, performing high-pressure treatment by adopting ultrahigh-pressure sterilization equipment, and performing ultrahigh-pressure 550MPa treatment for 3min to obtain a mango extracting solution; the feed-liquid ratio of the mango peel powder to the citric acid solution is 1: 15. The ultrasonic extraction conditions were as follows: the ultrasonic power is 80W, and the ultrasonic time is 20 min.
(3) Carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath on a mixed solution of mango peel pectin liquid with the mass fraction of 1.5% and sodium alginate solution with the mass fraction of 1.0% at 70 ℃ for 30min, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in chain triglyceride (MCT) in an oil phase, carrying out high-speed shearing for 2min, and carrying out ultrasonic crushing treatment for 2min by an ultrasonic crusher to obtain a film forming liquid; wherein the concentration of curcumin is 30 mg/mL.
(5) And (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring the film by adopting a tape casting method, drying at 25-35 ℃ for 12h, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (3) after the fresh-cut pineapples are packaged by the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5), irradiating by using LED visible light to realize an antibacterial effect, wherein the LED visible light is from an LED visible light source with the wavelength of 405 and 420nm, the output light power is more than 280mW, and the irradiation time by using the LED visible light is 20 min.
[ example 3 ]
The difference between the embodiment 3 and the embodiment 1 is that the illumination time of the LED is different, the illumination time of the embodiment 3 is 30min, and the rest conditions are the same.
The mango peel pectin-based photodynamic antibacterial film of example 3 is prepared by the following steps:
(1) blanching mango peel for inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with a citric acid solution to obtain a mixed solution, adjusting the pH value of the mixed solution to 2.5, performing ultrasonic extraction, performing high-pressure treatment by adopting ultrahigh-pressure sterilization equipment, and performing ultrahigh-pressure 550MPa treatment for 3min to obtain a mango extracting solution; the feed-liquid ratio of the mango peel powder to the citric acid solution is 1: 15. The ultrasonic extraction conditions were as follows: the ultrasonic power is 80W, and the ultrasonic time is 20 min.
(3) Carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath on a mixed solution of mango peel pectin liquid with the mass fraction of 1.5% and sodium alginate solution with the mass fraction of 1.0% at 70 ℃ for 30min, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in chain triglyceride (MCT) in an oil phase, carrying out high-speed shearing for 2min, and carrying out ultrasonic crushing treatment for 2min by an ultrasonic crusher to obtain a film forming liquid; wherein the concentration of curcumin is 30 mg/mL.
(5) And (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring the film by adopting a tape casting method, drying at 25-35 ℃ for 12h, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (3) after the fresh-cut pineapples are packaged by the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5), irradiating by using LED visible light to realize an antibacterial effect, wherein the LED visible light is from an LED visible light source with the wavelength of 405 and 420nm, the output light power is more than 280mW, and the irradiation time by using the LED visible light is 30 min.
[ example 4 ]
The difference between example 4 and example 1 is that the freshness keeping object was different, and the freshness keeping object of example 4 was mango, and the other conditions were the same.
The mango peel and fruit gum based photodynamic antibacterial film of example 4 is prepared by the following steps:
(1) blanching mango peel for inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with a citric acid solution to obtain a mixed solution, adjusting the pH value of the mixed solution to 2.5, performing ultrasonic extraction, performing high-pressure treatment by adopting ultrahigh-pressure sterilization equipment, and performing ultrahigh-pressure 550MPa treatment for 3min to obtain a mango extracting solution; the feed-liquid ratio of the mango peel powder to the citric acid solution is 1: 15. The ultrasonic extraction conditions were as follows: the ultrasonic power is 80W, and the ultrasonic time is 20 min.
(3) Carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath on a mixed solution of mango peel pectin liquid with the mass fraction of 1.5% and sodium alginate solution with the mass fraction of 1.0% at 70 ℃ for 30min, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in chain triglyceride (MCT) in an oil phase, carrying out high-speed shearing for 2min, and carrying out ultrasonic crushing treatment for 2min by an ultrasonic crusher to obtain a film forming liquid; wherein the concentration of curcumin is 30 mg/mL.
(5) And (5) after the film forming solution obtained in the step (4) is subjected to vacuum deaeration, pouring the film by adopting a tape casting method, drying the film at 25-35 ℃ for 12 hours, taking out the film, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (4) after fresh-cut mangoes are packaged by the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5), irradiating by using LED visible light to realize an antibacterial effect, wherein the LED visible light is derived from an LED visible light source with the wavelength of 405-420nm, the output light power is more than 280mW, and the irradiation time by using the LED visible light is 10 min.
[ example 5 ] A method for producing a polycarbonate
The differences between example 5 and example 1 are that the freshness keeping subjects were different, the freshness keeping subjects of example 5 were mangoes, the LED illumination time was different, and the illumination time of example 5 was 20min, and the other conditions were the same.
The mango peel and fruit gum based photodynamic antibacterial film of example 5 is prepared by the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with a citric acid solution to obtain a mixed solution, adjusting the pH value of the mixed solution to 2.5, performing ultrasonic extraction, performing high-pressure treatment by adopting ultrahigh-pressure sterilization equipment, and performing ultrahigh-pressure 550MPa treatment for 3min to obtain a mango extracting solution; the feed-liquid ratio of the mango peel powder to the citric acid solution is 1: 15. The ultrasonic extraction conditions were as follows: the ultrasonic power is 80W, and the ultrasonic time is 20 min.
(3) Carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath on a mixed solution of a mango peel pectin solution with the mass fraction of 1.5% and a sodium alginate solution with the mass fraction of 1.0% for 30min at 70 ℃, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, uniformly mixing, adding a photosensitizer curcumin dissolved in chain triglyceride (MCT) in an oil phase, carrying out high-speed shearing for 2min, and carrying out ultrasonic crushing treatment for 2min by an ultrasonic crusher to obtain a film forming solution; wherein the concentration of curcumin is 30 mg/mL.
(5) And (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring the film by adopting a tape casting method, drying at 25-35 ℃ for 12h, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (4) after fresh-cut mangoes are packaged by the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5), irradiating by using LED visible light to realize an antibacterial effect, wherein the LED visible light is derived from an LED visible light source with the wavelength of 405-420nm, the output light power is more than 280mW, and the irradiation time by using the LED visible light is 20 min.
[ example 6 ]
The difference between the embodiment 6 and the embodiment 1 is that the freshness keeping object is mango, the illumination time of the LED is different, the illumination time of the embodiment 6 is 30min, and the other conditions are the same.
The mango peel and fruit gum based photodynamic antibacterial film of example 6 is prepared by the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with a citric acid solution to obtain a mixed solution, adjusting the pH value of the mixed solution to 2.5, performing ultrasonic extraction, performing high-pressure treatment by adopting ultrahigh-pressure sterilization equipment, and performing ultrahigh-pressure 550MPa treatment for 3min to obtain a mango extracting solution; the feed-liquid ratio of the mango peel powder to the citric acid solution is 1: 15. The ultrasonic extraction conditions were as follows: the ultrasonic power is 80W, and the ultrasonic time is 20 min.
(3) Carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath on a mixed solution of mango peel pectin liquid with the mass fraction of 1.5% and sodium alginate solution with the mass fraction of 1.0% at 70 ℃ for 30min, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in chain triglyceride (MCT) in an oil phase, carrying out high-speed shearing for 2min, and carrying out ultrasonic crushing treatment for 2min by an ultrasonic crusher to obtain a film forming liquid; wherein the concentration of curcumin is 30 mg/mL.
(5) And (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring the film by adopting a tape casting method, drying at 25-35 ℃ for 12h, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (3) after the fresh-cut mangoes are packaged by the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5), irradiating by using LED visible light to realize an antibacterial effect, wherein the LED visible light is from an LED visible light source with the wavelength of 405 and 420nm, the output light power is more than 280mW, and the irradiation time by using the LED visible light is 30 min.
Comparative example 1
Comparative example 1 differs from example 1 in the absence of the step of sonication, high pressure treatment in step (2), with the remaining conditions being the same.
The mango peel and fruit gum based photodynamic antibacterial film of the comparative example 1 is prepared by the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with citric acid to obtain a mixed solution, and adjusting the pH value of the mixed solution to 2.5 to obtain a mango extracting solution;
(3) carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath on a mixed solution of mango peel pectin liquid with the mass fraction of 1.5% and sodium alginate solution with the mass fraction of 1.0% at 70 ℃ for 30min, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in chain triglyceride (MCT) in an oil phase, carrying out high-speed shearing for 2min, and carrying out ultrasonic crushing treatment for 2min by an ultrasonic crusher to obtain a film forming liquid; wherein the concentration of curcumin is 30 mg/mL.
(5) And (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring the film by adopting a tape casting method, drying at 25-35 ℃ for 12h, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (4) after the fresh-cut pineapples are packaged by the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5), irradiating by using LED visible light to realize an antibacterial effect, wherein the LED visible light is derived from an LED visible light source with the wavelength of 405-420nm, the output light power is more than 280mW, and the irradiation time by using the LED visible light is 10 min.
Comparative example 2
The difference between the comparative example 2 and the example 1 is that in the step of ultrasonic and high-pressure treatment in the step (2), the high-pressure condition is different from the ultrasonic condition, and the high-pressure treatment is carried out for 8min under the pressure of 400 MPa; the ultrasonic power is 40W, and the ultrasonic time is 30 min. The rest conditions are the same.
The mango peel pectin based photodynamic antibacterial film of comparative example 2 was prepared by the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with a citric acid solution to obtain a mixed solution, adjusting the pH value of the mixed solution to 2.5, performing ultrasonic extraction, performing high-pressure treatment by adopting ultrahigh-pressure sterilization equipment, and performing ultrahigh-pressure 400MPa treatment for 1min to obtain a mango extracting solution; the feed-liquid ratio of the mango peel powder to the citric acid solution is 1: 15. The ultrasonic extraction conditions were as follows: the ultrasonic power is 40W, and the ultrasonic time is 10 min.
(3) Carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath on a mixed solution of mango peel pectin liquid with the mass fraction of 1.5% and sodium alginate solution with the mass fraction of 1.0% at 70 ℃ for 30min, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in chain triglyceride (MCT) in an oil phase, carrying out high-speed shearing for 2min, and carrying out ultrasonic crushing treatment for 2min by an ultrasonic crusher to obtain a film forming liquid; wherein the concentration of curcumin is 30 mg/mL.
(5) And (5) after the film forming solution obtained in the step (4) is subjected to vacuum deaeration, pouring the film by adopting a tape casting method, drying the film at 25-35 ℃ for 12 hours, taking out the film, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (3) after the fresh-cut pineapples are packaged by the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5), irradiating by using LED visible light to realize an antibacterial effect, wherein the LED visible light is from an LED visible light source with the wavelength of 405 and 420nm, the output light power is more than 280mW, and the irradiation time by using the LED visible light is 10 min.
Comparative example 3
The difference between the comparative example 3 and the example 1 is that in the step of ultrasonic high-pressure treatment in the step (2), the high-pressure condition is different from the ultrasonic condition, and the high-pressure treatment is carried out for 1min at 650 MPa; the ultrasonic power is 120W, the ultrasonic time is 10min, and the rest conditions are the same.
The mango peel pectin based photodynamic antibacterial film of comparative example 3 is prepared by the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with citric acid to obtain a mixed solution, adjusting the pH value of the mixed solution to 2.5, performing ultrasonic extraction, performing high-pressure treatment by adopting ultrahigh-pressure sterilization equipment, and performing ultrahigh-pressure treatment at 650MPa for 1min to obtain a mango extracting solution; the feed-liquid ratio of the mango peel powder to the citric acid solution is 1: 15. The ultrasonic extraction conditions were as follows: the ultrasonic power is 120W, and the ultrasonic time is 10 min.
(3) Carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath on a mixed solution of a mango peel pectin solution with the mass fraction of 1.5% and a sodium alginate solution with the mass fraction of 1.0% for 30min at 70 ℃, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, uniformly mixing, adding a photosensitizer curcumin dissolved in chain triglyceride (MCT) in an oil phase, carrying out high-speed shearing for 2min, and carrying out ultrasonic crushing treatment for 2min by an ultrasonic crusher to obtain a film forming solution; wherein the concentration of curcumin is 30 mg/mL.
(5) And (5) after the film forming solution obtained in the step (4) is subjected to vacuum deaeration, pouring the film by adopting a tape casting method, drying the film at 25-35 ℃ for 12 hours, taking out the film, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (3) after the fresh-cut pineapples are packaged by the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5), irradiating by using LED visible light to realize an antibacterial effect, wherein the LED visible light is from an LED visible light source with the wavelength of 405 and 420nm, the output light power is more than 280mW, and the irradiation time by using the LED visible light is 10 min.
Comparative example 4
The comparative example 4 is different from example 1 in that the step of adding curcumin, high-speed shearing, ultrasonication was omitted in step (4), and the rest conditions were the same.
The mango peel pectin-based photodynamic antibacterial film of comparative example 4 was prepared by the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with citric acid to obtain a mixed solution, adjusting the pH value of the mixed solution to 2.5, performing ultrasonic extraction, performing high-pressure treatment by adopting ultrahigh-pressure sterilization equipment, and performing ultrahigh-pressure 550MPa treatment for 3min to obtain a mango extracting solution; the feed-liquid ratio of the mango peel powder to the citric acid solution is 1: 15. The ultrasonic extraction conditions were as follows: the ultrasonic power is 80W, and the ultrasonic time is 20 min.
(3) Carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath for 30min at 70 ℃ on a mixed solution of 1.5% by mass of mango peel pectin liquid and 1.0% by mass of sodium alginate solution, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, and uniformly mixing to obtain a film forming liquid;
(5) and (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring the film by adopting a tape casting method, drying at 25-35 ℃ for 12h, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (3) after the fresh-cut pineapples are packaged by the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5), irradiating by using LED visible light to realize an antibacterial effect, wherein the LED visible light is from an LED visible light source with the wavelength of 405 and 420nm, the output light power is more than 280mW, and the irradiation time by using the LED visible light is 10 min.
Comparative example 5
Comparative example 5 differs from example 1 in the absence of a step of LED illumination in step (6), with the same remaining conditions.
The mango peel pectin-based photodynamic antibacterial film of comparative example 5 is prepared by the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with citric acid to obtain a mixed solution, adjusting the pH value of the mixed solution to 2.5, performing ultrasonic extraction, performing high-pressure treatment by adopting ultrahigh-pressure sterilization equipment, and performing ultrahigh-pressure 550MPa treatment for 3min to obtain a mango extracting solution; the feed-liquid ratio of the mango peel powder to the citric acid solution is 1: 15. The ultrasonic extraction conditions were as follows: the ultrasonic power is 80W, and the ultrasonic time is 20 min.
(3) Carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath on a mixed solution of mango peel pectin liquid with the mass fraction of 1.5% and sodium alginate solution with the mass fraction of 1.0% at 70 ℃ for 30min, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in chain triglyceride (MCT) in an oil phase, carrying out high-speed shearing for 2min, and carrying out ultrasonic crushing treatment for 2min by an ultrasonic crusher to obtain a film forming liquid; wherein the concentration of curcumin is 30 mg/mL.
(5) And (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring the film by adopting a tape casting method, drying at 25-35 ℃ for 12h, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (5) packaging the fresh-cut pineapples by using the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5).
Comparative example 6
The difference between comparative example 6 and example 1 is that the ultrasonic and high-pressure treatment step is omitted in step (2), and the preservation subjects were different, and the preservation subject of comparative example 6 was mango, and the other conditions were the same.
The mango peel pectin-based photodynamic antibacterial film of comparative example 6 is prepared by the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with a citric acid solution to obtain a mixed solution, and adjusting the pH value of the mixed solution to 2.5 to obtain a mango extracting solution;
(3) carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath on a mixed solution of mango peel pectin liquid with the mass fraction of 1.5% and sodium alginate solution with the mass fraction of 1.0% at 70 ℃ for 30min, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in chain triglyceride (MCT) in an oil phase, carrying out high-speed shearing for 2min, and carrying out ultrasonic crushing treatment for 2min by an ultrasonic crusher to obtain a film forming liquid; wherein the concentration of curcumin is 30 mg/mL.
(5) And (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring the film by adopting a tape casting method, drying at 25-35 ℃ for 12h, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (4) after fresh-cut mangoes are packaged by the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5), irradiating by using LED visible light to realize an antibacterial effect, wherein the LED visible light is derived from an LED visible light source with the wavelength of 405-420nm, the output light power is more than 280mW, and the irradiation time by using the LED visible light is 10 min.
Comparative example 7
The difference between the comparative example 7 and the example 1 is that the step of adding curcumin, high-speed shearing and ultrasonic crushing is omitted in the step (4), the preservation objects are different, the preservation object of the comparative example 7 is mango, and the rest conditions are the same.
The mango peel pectin-based photodynamic antibacterial film of comparative example 7 is prepared by the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with a citric acid solution to obtain a mixed solution, adjusting the pH value of the mixed solution to 2.5, performing ultrasonic extraction, performing high-pressure treatment by adopting ultrahigh-pressure sterilization equipment, and performing ultrahigh-pressure 550MPa treatment for 3min to obtain a mango extracting solution; the feed-liquid ratio of the mango peel powder to the citric acid solution is 1: 15. The ultrasonic extraction conditions were as follows: the ultrasonic power is 80W, and the ultrasonic time is 20 min.
(3) Carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath for 30min at 70 ℃ on a mixed solution of 1.5% by mass of mango peel pectin liquid and 1.0% by mass of sodium alginate solution, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, and uniformly mixing to obtain a film forming liquid;
(5) and (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring the film by adopting a tape casting method, drying at 25-35 ℃ for 12h, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (3) after the fresh-cut mangoes are packaged by the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5), irradiating by using LED visible light to realize an antibacterial effect, wherein the LED visible light is from an LED visible light source with the wavelength of 405 and 420nm, the output light power is more than 280mW, and the irradiation time by using the LED visible light is 10 min.
Comparative example 8
The difference between comparative example 8 and example 1 is that the step of LED illumination is absent in step (6), and the preservation subjects were different, and the preservation subject of comparative example 8 was mango, and the other conditions were the same.
The mango peel and fruit gum based photodynamic antibacterial film of comparative example 8 is prepared by the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder; the temperature of the blanching enzyme deactivation is 85-95 ℃, and the blanching enzyme deactivation time is 2-5 min.
(2) Mixing the mango peel powder prepared in the step (1) with a citric acid solution to obtain a mixed solution, adjusting the pH value of the mixed solution to 2.5, performing ultrasonic extraction, performing high-pressure treatment by adopting ultrahigh-pressure sterilization equipment, and performing ultrahigh-pressure 550MPa treatment for 3min to obtain a mango extracting solution; the feed-liquid ratio of the mango peel powder to the citric acid solution is 1: 15. The ultrasonic extraction conditions were as follows: the ultrasonic power is 80W, and the ultrasonic time is 20 min.
(3) Carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) carrying out water bath on a mixed solution of mango peel pectin liquid with the mass fraction of 1.5% and sodium alginate solution with the mass fraction of 1.0% at 70 ℃ for 30min, fully dissolving and mixing, adding 1.2% of plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in chain triglyceride (MCT) in an oil phase, carrying out high-speed shearing for 2min, and carrying out ultrasonic crushing treatment for 2min by an ultrasonic crusher to obtain a film forming liquid; wherein the concentration of curcumin is 30 mg/mL.
(5) And (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring the film by adopting a tape casting method, drying at 25-35 ℃ for 12h, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film, namely the mango peel pectin-based photodynamic antibacterial film.
(6) And (5) packaging fresh-cut mangoes by adopting the mango peel and fruit gum base photodynamic antibacterial film obtained in the step (5).
Evaluation of Effect and detection of Performance
1. The packaged fresh-cut fruits of examples 1 to 6 and comparative examples 1 to 8 were refrigerated for 9 days, and then measured for the microbial indicator, the weight loss rate, and the Water Vapor Permeability (WVP) of the film of pineapple and mango. In addition, fresh-cut pineapples and mangoes which are not packaged are used as blank control groups; fresh-cut fruits were packaged with commercially available PVC wrap as a conventional control. Wherein, the microbial indexes are obtained by calculating the total number of bacterial colonies of the fresh-cut fruits of the experimental group and the blank control group by adopting a plate counting method. Weight loss (%) - (weight per time-original weight)/original weight 100%. The water vapor permeability is measured by a cup-like method. See the table below for the test results.
Table 1 shows the fresh-keeping effects on fresh-cut pineapples of examples 1 to 3 and comparative examples 1 to 5
Figure BDA0003700925760000151
Figure BDA0003700925760000161
The results show that in examples 2 and 3, compared with example 1, the illumination time of the LED visible light is 20min and 30min, the illumination efficiency is increased, the sterilization effect is obviously improved, and as shown in table 1, the total number of colonies of the fresh-cut pineapples and the number of mould yeasts are reduced; compared with the embodiment 1, the comparative examples 1-3 have the advantages that ultrasonic and ultrahigh pressure auxiliary means are not adopted in the mango peel pectin extraction process, or the ultrasonic and high pressure conditions are not in the condition range limited by the invention, the bacteria reduction effect is poorer than that of the embodiment 1, the water vapor permeability of the preservative film is higher than that of the embodiment 1, and the mango peel pectin extracted by adopting the ultrasonic and ultrahigh pressure auxiliary means has improved structural properties, stronger intermolecular force with other film components such as curcumin and the like, the possibility of water vapor molecules extruding into polymer chains is reduced, the moisture permeability is correspondingly reduced, the water vapor permeability of the preservative film is relatively reduced, the weight loss rate is reduced, and the moisture of fresh-cut fruits is kept. Compared with the embodiment 1, the comparative example 2 is a simple mango peel pectin and sodium alginate film, and has insufficient bacteria reduction effect; comparative example 3 compared with example 1, the LED-treated sample was not irradiated with visible light, and was not different from the untreated blank control sample, and the fresh-keeping effect was not good.
Table 2 shows the fresh-keeping effects on fresh-cut mangoes of examples 4 to 6 and comparative examples 6 to 8
Figure BDA0003700925760000162
Figure BDA0003700925760000171
The results show that in examples 5 and 6, compared with example 4, the illumination time of the LED visible light is 20min and 30min, the illumination efficiency is increased, the sterilization effect is obviously improved, and as shown in Table 2, the total number of colonies of fresh-cut mangoes and the number of mould yeasts are reduced; compared with the embodiment 4, the comparative example 6 has the advantages that ultrasonic and ultrahigh pressure auxiliary means are not adopted in the mango peel pectin extraction process, the bacteria reduction effect is poorer than that of the embodiment 4, and the water vapor permeability of the preservative film is higher than that of the embodiment 4. Compared with the embodiment 5, the comparative example 7 is a film made of pure mango peel pectin and sodium alginate, and has insufficient bacteria reduction effect; comparative example 8 compared with example 5, the LED-treated sample was not irradiated with visible light, and was not different from the untreated blank control sample, and the fresh-keeping effect was not good.
2. Effect on the gel texture Properties of mango Peel pectin
The gel texture characteristics of the mango peel pectin obtained by the steps (1) to (3) of the example 1 and the comparative example 1 are measured by a texture analyzer, and the detection results are shown in a table 3:
TABLE 3 comparison of pectin gelling Properties of mango peels
Figure BDA0003700925760000172
3. Influence on emulsifying property of mango peel pectin
The emulsifying characteristics of the mango peel pectin obtained by the steps (1) to (3) of the example 1 and the comparative example 1 are detected, and the detection method comprises the following steps:
preparing an emulsion: preparing 20mL of 1.8% pectin solution with 0.01mol/L citric acid solution, adjusting pH to 3.0, 4.0, 5.0, and 6.0 with 0.1mol/L hydrochloric acid, respectively, adding 5mL peanut oil, magnetically stirring for 3min, and homogenizing at 24000rpm for 1min with a high-speed homogenizer.
Determination of the emulsifying Activity: placing fresh emulsion in a centrifugal tube with scale, centrifuging at room temperature for 5min (3000rpm), and measuring total emulsion volume and V 1 And an emulsion layer V 2 According to the formula EA ═ V 2 /V 1 X 100% calculation of emulsifying activity EA (%); .
Determination of emulsion stability: standing the emulsion at room temperature for 7 days, centrifuging at 3000rpm for 5min, and measuring emulsion layer volume V 3 . According to the original emulsion layer V 2 According to the following formula ES ═ V 3 /V 2 X 100% emulsion stability ES (%);
determination of milk analysis index: taking 10mL of fresh emulsion and a glass colorimetric tube, and recording the total height H of the initial emulsion 1 After standing at room temperature for 7 days, the height H of the whey layer was measured 2 According to the formula CI ═ H 2 /H 1 X 100% milk analysis index CI (%).
TABLE 4 comparison of mango peel pectin emulsification Properties
Figure BDA0003700925760000181
The method utilizes ultrasonic and ultrahigh pressure assisted acid hydrolysis technology to extract mango peel pectin, improves the gelling property and the emulsifying property of the pectin, coats the photosensitizer curcumin in the composite film, improves the stability and the slow release property of the photosensitizer curcumin to a certain extent, prepares the photodynamic antibacterial film, is used together with a green, economic and environment-friendly visible light LED light source, and can effectively reduce the microbial count and the juice loss rate of fresh-cut fruits.
4. The physical and chemical index changes of the fresh-cut fruits before and after the treatment of the fresh-cut fruits by the photodynamic antibacterial film
Taking pineapples as an example, fresh-cut fruits of pineapples are divided into two groups, one group is a blank control group without any treatment, and the other group is subjected to packaging and antibacterial fresh-keeping treatment by the photodynamic antibacterial fresh-keeping film prepared in the example 1. The fresh-cut pineapples were then measured for physicochemical properties with respect to soluble solids, titratable acid, hardness, etc., and the results are shown in table 5.
TABLE 5 variation of physical and chemical indexes of fresh-cut fruits before and after treatment
Soluble solids/% Titratable acid/%) hardness/N/100 g
Photodynamic experimental group 12.3 0.42 30.56
Blank control group 12.2 0.42 23.56
The results show that the soluble solid and titratable acid of the fresh-cut fruits have no significant change before and after the packaging of the photodynamic antibacterial preservative film, the hardness is higher than that of a control group, and the photodynamic antibacterial preservative film has no influence on the physicochemical properties of the fresh-cut fruits and is even beneficial to maintaining the texture of the pineapples.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims (10)

1. A preparation method of a mango peel and fruit gum base photodynamic antibacterial film applied to fresh keeping of fresh-cut fruits is characterized by comprising the following steps:
(1) blanching mango peel, inactivating enzyme, and freeze-drying to obtain mango peel powder;
(2) mixing the mango peel powder prepared in the step (1) with a citric acid solution to obtain a mixed solution, adjusting the pH value of the mixed solution, performing ultrasonic extraction, and performing ultrahigh pressure treatment to obtain a mango extracting solution;
(3) carrying out solid-liquid separation on the mango extracting solution obtained in the step (2), mixing a liquid phase component obtained after the solid-liquid separation with ethanol, and precipitating to obtain mango peel pectin; naturally cooling to room temperature, filtering and separating, taking precipitate, and freeze-drying to obtain mango peel pectin;
(4) mixing the mango peel pectin solution obtained in the step (3) with sodium alginate, carrying out water bath on the obtained mixed solution, fully dissolving and mixing, adding plasticizer glycerol, uniformly mixing, adding photosensitizer curcumin dissolved in an oil phase, carrying out high-speed shearing, and carrying out ultrasonic crushing treatment by an ultrasonic crusher to obtain a film forming solution;
(5) and (4) after the film forming liquid obtained in the step (4) is subjected to vacuum defoaming, pouring a film by adopting a tape casting method, drying, taking out, and uncovering the film to obtain the mango peel pectin-curcumin composite film.
2. The preparation method of the mango peel and fruit gum based photodynamic antibacterial film as claimed in claim 1, wherein in the step (1), the temperature of the blanching enzyme deactivation is 85-95 ℃, and the time of the blanching enzyme deactivation is 2-5 min.
3. The preparation method of the mango peel pectin based photodynamic antibacterial film as claimed in claim 1, wherein in the step (2), the material-liquid ratio of the mango peel powder to the citric acid solution is 1 (10-20); the pH value of the mixed solution is adjusted to 2.0-3.0.
4. The method for preparing the mango peel pectin based photodynamic antibacterial film according to claim 1, wherein in the step (2), the ultrasonic extraction conditions are as follows: the ultrasonic power is 60-100W, and the ultrasonic time is 15-25 min; the equipment adopted by the high-pressure treatment is ultrahigh-pressure sterilization equipment, and the high-pressure treatment condition is that the high-pressure treatment is carried out for 2-5min at 550 MPa.
5. The preparation method of the mango peel pectin based photodynamic antibacterial film according to claim 1, wherein in the step (4), the mango peel pectin obtained in the step (3) and sodium alginate are dissolved in water to obtain a mixed solution of 1-1.5% by mass of the mango peel pectin and 0.8-1.25% by mass of the sodium alginate.
6. The method for preparing the mango peel and fruit gum based photodynamic antibacterial film as claimed in claim 1, wherein in the step (4), the photosensitizer curcumin dissolved in the oil phase is prepared as follows: the oil phase adopted is medium chain triglyceride MCT; the content of the photosensitizer curcumin is 25-35 mg/ml.
7. The method for preparing the mango peel pectin based photodynamic antibacterial film according to claim 1, wherein in the step (4), the water bath conditions are as follows: the water bath temperature is 65-75 deg.C, and the water bath time is 25-35 min; the mass fraction of the plasticizer glycerol is 1-1.2%; the high-speed shearing time is 2 min; the crushing treatment time is 2 min.
8. The method for preparing the mango peel and pectin based photodynamic antibacterial film according to claim 1, wherein in the step (5), the drying temperature is 25-35 ℃, and the drying time is more than 12 h.
9. The mango peel pectin based photodynamic antibacterial film according to any one of claims 1 to 8, which is prepared by the preparation method of the mango peel pectin based photodynamic antibacterial film according to any one of claims 1 to 8.
10. The method for applying the mango peel and fruit gum based photodynamic antibacterial film to fresh-cut fruits according to claim 9 is characterized in that after the mango peel and fruit gum based photodynamic antibacterial film is used for packaging the fresh-cut fruits, LED visible light is used for irradiating the fresh-cut fruits; the LED visible light is from an LED visible light source with the wavelength of 405-420nm, the output light power is more than 280mW, and the irradiation time is 10-40 min.
CN202210692852.6A 2022-06-17 2022-06-17 Mango peel pectin-based photodynamic antibacterial film and preparation method and application thereof Active CN115028865B (en)

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