CN115025027A - Biological factor eyelash nourishing liquid - Google Patents
Biological factor eyelash nourishing liquid Download PDFInfo
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- CN115025027A CN115025027A CN202210672094.1A CN202210672094A CN115025027A CN 115025027 A CN115025027 A CN 115025027A CN 202210672094 A CN202210672094 A CN 202210672094A CN 115025027 A CN115025027 A CN 115025027A
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- extract
- hair
- explant
- astragalus
- propylene glycol
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 54
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- AXTGDCSMTYGJND-UHFFFAOYSA-N 1-dodecylazepan-2-one Chemical compound CCCCCCCCCCCCN1CCCCCC1=O AXTGDCSMTYGJND-UHFFFAOYSA-N 0.000 claims abstract description 26
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
- A61Q1/02—Preparations containing skin colorants, e.g. pigments
- A61Q1/10—Preparations containing skin colorants, e.g. pigments for eyes, e.g. eyeliner, mascara
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Birds (AREA)
- Developmental Biology & Embryology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Dermatology (AREA)
- Environmental Sciences (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a biological factor eyelash nourishing liquid, which comprises astragalus extract, dried ginger extract, vitamin E, glycerol, azone, propylene glycol, sodium hydroxide and lactic acid. The astragalus root explant is infected by agrobacterium rhizogenes containing FGF-10 gene Ri plasmid, FGF-10 protein can be generated, activity of germinal cells and environment of hair follicles are improved, activity and content of hair follicle growth factors are improved, hair mother cells and hair papilla cells are stimulated to regenerate, and hair regeneration speed can be increased by combining drug property of astragalus root. The gingerol, zingiberone and other substances contained in the dried ginger can promote blood circulation, increase hair follicle nutrition, stimulate hair follicles and promote hair regeneration. The azone and the propylene glycol can generate synergistic action, act on a cell lipid bilayer to increase the fluidity of the bilayer, and promote the diffusion of FGF protein and medicaments between cells, thereby increasing the penetration of effective substances. The invention has the characteristics of safety and quick response.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a biological factor eyelash nourishing liquid.
Background
Pregnant and lactating women have alopecia and eyelash loss. During the gestational period, the fetus develops with a large demand for the mother, and in the process, the deficiency of trace elements is easy to occur, so that the physical condition is reduced, the endocrine is disturbed, and eyelashes are removed. The nutrition demand of the infant is gradually increased along with the growth of the infant in the lactation period, and the breast feeding causes poor nutrition supply of the mother and also causes the loss of hair or eyelashes.
Eyelashes fall off and seriously influence the image, can also bring extra pressure for the women, and bad mood has harmful effects to child development in pregnancy, and the women of lactation period also can be more anxious, increases postpartum depression risk. The women in pregnancy and lactation need to take a lot of care, so that eyelash nourishing liquid capable of promoting eyelash to grow rapidly is needed. The hair loss is researched more in the market, and the eyelash shedding is researched less in the pregnancy and lactation period.
Therefore, it is necessary to design a safe and fast-acting biological factor eyelash nourishing liquid and a preparation method thereof.
Disclosure of Invention
The invention aims to provide a biological factor eyelash nourishing liquid and a preparation method thereof, which aim to solve the problems in the background technology.
In order to solve the technical problems, the invention provides the following technical scheme: a biological factor eyelash nourishing liquid comprises radix astragali extract, Zingiberis rhizoma extract, vitamin E, glycerol, azone, propylene glycol, sodium hydroxide, and lactic acid.
According to the technical scheme, the biological factor eyelash nourishing liquid comprises, by mass, 3-5 parts of astragalus extract, 1-3 parts of dried ginger extract, 0.5-2 parts of vitamin E, 2-8 parts of glycerol, 0.8-3.5 parts of azone, 0.4-3 parts of propylene glycol, 0.1-0.6 part of sodium hydroxide and 0.5-4 parts of lactic acid.
According to the technical scheme, the biological factor eyelash nourishing liquid comprises, by mass, 5 parts of astragalus extract, 1.5 parts of dried ginger extract, 0.5 part of vitamin E, 6 parts of glycerol, 1.2 parts of azone, 1.5 parts of propylene glycol, 0.3 part of sodium hydroxide and 1.6 parts of lactic acid.
The biological factor eyelash nourishing liquid and its preparation process includes the following steps:
the method comprises the following steps: preparing an astragalus extract: cleaning hairy root of radix astragali, grinding in liquid nitrogen in mortar, adding deionized water, stirring, standing for 3-5 hr, centrifuging at 8000-;
step two: preparing a dried ginger extract: soaking dried ginger powder in 90% ethanol as solvent for 24 hr, slowly percolating at a speed of 1-3 ml per minute, collecting primary percolate 850ml, storing in another container, percolating until the percolate is nearly colorless and the fragrance and pungency of ginger are thin, collecting percolate, concentrating at below 60 deg.C to obtain soft extract, adding primary percolate, mixing, filtering, standing, clarifying, and filtering to obtain ginger fluid extract;
step three: grinding 10% glycerol, adding appropriate amount of purified water, swelling, adding radix astragali extract, Zingiberis rhizoma extract, propylene glycol, azone, propylene glycol, VE, and lactic acid, stirring, and adjusting pH to 5.5-6.5 with sodium hydroxide.
According to the technical scheme, the astragalus extract is extracted from explant hairy roots, and the explant can be roots, stems and leaves.
According to the technical scheme, the astragalus root explant is infected by agrobacterium rhizogenes for the hairy root of the explant: taking stem section as explant, pH of culture medium is 5-6, sucrose concentration is 2-5%, explant age is 30-35D, ultrasonic treatment time is 4s, pre-culture and co-culture are 2-3D, infection time is 5-10min, and exogenous hormone is added at 0.1 mg/mLNAA.
According to the technical scheme, the agrobacterium rhizogenes is one of A4, 15834, 1025 and 1000 strains.
According to the technical scheme, the Ri plasmid of the agrobacterium rhizogenes contains FGF-10 genes, FGF10 genes are amplified through PCR, and the Ri plasmid is connected after double enzyme digestion.
According to the technical scheme, the astragalus mongholicus hairy roots are subjected to PCR detection: according to the designed FGF-10 gene primer, the same band as the gene primer appears in the band (627 bp), but the untransformed astragalus explant does not amplify the band, so that the Ri plasmid of the agrobacterium is integrated into the genome of the hairy root.
According to the technical scheme, the astragalus mongholicus hairy roots are transferred into a liquid culture medium for amplification culture: and (5) culturing by using an MS liquid culture medium with sucrose concentration of 30g/L and adding JA with 30 mu mol/L for 25-35D.
Astragalus root, a Chinese medicinal material of leguminosae. The main functional indications are as follows: has effects in invigorating qi, consolidating superficial resistance, expelling toxic materials, promoting pus discharge, promoting urination, promoting granulation, and promoting hair growth.
FGF10 has wide clinical application, mainly including promoting injury repair (such as burn, corneal injury, lung injury, etc.), has certain effect on facial red blood streak, and can promote proliferation and differentiation of stem cells. Especially, FGF10 can improve the activity of hair-growing cells and the environment of hair follicles, increase the activity and content of hair follicle growth factors, and stimulate the regeneration of hair mother cells and hair papilla cells, thereby achieving the effect of promoting hair regeneration.
The astragalus root extract prepared by infecting the astragalus root explant by agrobacterium rhizogenes containing the Ri plasmid of the FGF-10 gene can generate FGF-10 protein in the astragalus root explant, and can promote the rapid growth of hair and the regeneration speed by combining the drug property of the astragalus root.
Gingerol substances such as gingerol and zingiberone contained in Zingiberis rhizoma can dilate blood vessel and promote blood circulation, and they are all easily dissolved in ethanol. The ginger fluid extract is prepared from ethanol, and has the functions of expanding subcutaneous blood vessels, promoting blood circulation, increasing hair follicle nutrition, stimulating hair follicles and promoting hair regeneration.
Azone (Azone) is an excellent percutaneous absorption enhancer. It mainly acts on cell lipid bilayer to increase the fluidity of bilayer, reduce phase transition temperature, promote the diffusion of FGF protein and medicine between cells, and thus increase the penetration of medicine. Azone has transdermal enhancing effect on many lipophilic and hydrophilic drugs, and can produce synergistic effect when used with Propylene Glycol (PG). The azone has the best transdermal enhancing effect especially on low-concentration medicaments, and has low side effect. Therefore, azone and PG are selected as the composite transdermal enhancer.
Since the extract of Zingiberis rhizoma is lipophilic, while glycerin has thickening, moisturizing, lubricating, and carrier solvents, PG is commonly used as a solvent, cosolvent, humectant, and preservative in pharmaceutical formulations, and has permeation-promoting effects, glycerin and PG are added to increase the dissolution and uniform dispersion of the drug.
VE has high boiling point, is stable to heat, is easy to dissolve in ethanol, is non-toxic, has obvious effect of resisting skin aging, and can improve skin roughness, scales, smoothness, wrinkles and the like, so VE is selected as an antioxidant.
Compared with the prior art, the invention has the following beneficial effects: in the invention, the raw materials are mixed,
(1) the astragalus root explant is infected by agrobacterium rhizogenes containing the Ri plasmid of the FGF-10 gene, FGF-10 protein can be generated in the astragalus root explant, the activity of germinal cells and the environment of hair follicles are improved, the activity and the content of hair follicle growth promoting factors are improved, and the regeneration of hair mother cells and hair papilla cells is stimulated, so that the hair regeneration is promoted, and the hair regeneration speed can be increased by combining the drug property of the astragalus root;
(2) gingerol substances such as gingerol and zingiberone contained in the dried ginger can expand blood vessels and promote blood circulation, are easily dissolved in ethanol, and are prepared into ginger fluid extract by using the ethanol, so that the ginger fluid extract mainly has the effects of expanding subcutaneous blood vessels, promoting blood circulation, increasing hair follicle nutrition, stimulating hair follicles and promoting hair regeneration;
(3) azone is an excellent percutaneous absorption promoter, and the Propylene Glycol (PG) can generate a synergistic effect when used together, mainly acts on a cell lipid bilayer to increase the mobility of the bilayer, reduce the phase transition temperature, and promote the diffusion of FGF protein and medicaments between cells, thereby increasing the permeation of effective substances.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
The invention provides the technical scheme that: a biological factor eyelash nourishing liquid comprises radix astragali extract, Zingiberis rhizoma extract, vitamin E, glycerol, azone, propylene glycol, sodium hydroxide, and lactic acid.
According to the technical scheme, the biological factor eyelash nourishing liquid comprises, by mass, 3-5 parts of astragalus extract, 1-3 parts of dried ginger extract, 0.5-2 parts of vitamin E, 2-8 parts of glycerol, 0.8-3.5 parts of azone, 0.4-3 parts of propylene glycol, 0.1-0.6 part of sodium hydroxide and 0.5-4 parts of lactic acid.
According to the technical scheme, the biological factor eyelash nourishing liquid comprises, by mass, 5 parts of astragalus extract, 1.5 parts of dried ginger extract, 0.5 part of vitamin E, 6 parts of glycerol, 1.2 parts of azone, 1.5 parts of propylene glycol, 0.3 part of sodium hydroxide and 1.6 parts of lactic acid.
A biological factor eyelash nourishing liquid and a preparation method thereof comprise the following specific steps:
the method comprises the following steps: preparing the astragalus extract: cleaning hairy root of radix astragali, grinding in liquid nitrogen in mortar, adding deionized water, stirring, standing for 3-5 hr, centrifuging at 8000-;
step two: preparing a dried ginger extract: soaking dried ginger powder in 90% ethanol as solvent for 24 hr, slowly percolating at a speed of 1-3 ml per minute, collecting primary percolate 850ml, storing in another container, percolating until the percolate is nearly colorless and the fragrance and pungency of ginger are thin, collecting percolate, concentrating at below 60 deg.C to obtain soft extract, adding primary percolate, mixing, filtering, standing, clarifying, and filtering to obtain ginger fluid extract;
step three: grinding 10% glycerol, adding appropriate amount of purified water, swelling, adding radix astragali extract, Zingiberis rhizoma extract, propylene glycol, azone, propylene glycol, VE, and lactic acid, stirring, and adjusting pH to 5.5-6.5 with sodium hydroxide.
According to the technical scheme, the astragalus extract is extracted from explant hairy roots, and the explant can be roots, stems and leaves.
According to the technical scheme, the astragalus root explant is infected by agrobacterium rhizogenes for the hairy root of the explant: taking stem section as explant, pH of culture medium is 5-6, sucrose concentration is 2-5%, explant age is 30-35D, ultrasonic treatment time is 4s, preculture and co-culture are 2-3D, infection time is 5-10min, and exogenous hormone is added at 0.1 mg/mLNAA.
According to the technical scheme, the agrobacterium rhizogenes is one of A4, 15834, 1025 and 1000 strains.
According to the technical scheme, the Ri plasmid of the agrobacterium rhizogenes contains an FGF-10 gene, the FGF10 gene is amplified through PCR, and the Ri plasmid is connected after double enzyme digestion.
According to the technical scheme, the astragalus mongholicus hairy roots are subjected to PCR detection: according to the designed FGF-10 gene primer pair, the same band as the gene primer appears in the band (627 bp), but the untransformed astragalus explant does not amplify the band, thus proving that the Ri plasmid of the agrobacterium has been integrated into the genome of the hairy root.
According to the technical scheme, the astragalus mongholicus hairy roots are transferred into a liquid culture medium for amplification and multiplication culture: culturing 25-35D by using MS liquid culture medium with sucrose concentration of 30g/L and adding JA of 30 mu mol/L.
Case 1:
a biological factor eyelash nourishing liquid comprises radix astragali extract, Zingiberis rhizoma extract, vitamin E, glycerol, azone, propylene glycol, sodium hydroxide, and lactic acid.
According to the mass ratio, the astragalus extract is 5 parts, the dried ginger extract is 1.5 parts, the vitamin E is 0.5 part, the glycerol is 6 parts, the azone is 1.2 parts, the propylene glycol is 1.5 parts, the sodium hydroxide is 0.3 part, and the lactic acid is 1.6 parts.
The biological factor eyelash nourishing liquid and its preparation process includes the following steps:
the method comprises the following steps: preparing an astragalus extract: cleaning hairy root of radix astragali, grinding in liquid nitrogen in mortar, adding deionized water, stirring, standing for 4 hr, centrifuging at 10000rpm for 30min, and collecting supernatant to obtain radix astragali extract;
step two: preparing a dried ginger extract: soaking dried ginger powder in 90% ethanol as solvent for 24 hr, slowly percolating at speed of 2ml per minute, collecting primary percolate 850ml, storing in another container, percolating until the percolate is nearly colorless and the fragrance and pungency of ginger are thin, collecting percolate, concentrating at below 60 deg.C to obtain soft extract, adding primary percolate, mixing, filtering, standing, clarifying, and filtering to obtain ginger fluid extract;
step three: grinding 10% glycerol, adding appropriate amount of purified water, swelling, adding radix astragali extract, Zingiberis rhizoma extract, propylene glycol, azone, propylene glycol, VE, and lactic acid, stirring, and adjusting pH to 5.5-6.5 with sodium hydroxide.
According to the technical scheme, the astragalus extract is extracted from explant hairy roots, and roots are selected as explants.
According to the technical scheme, the astragalus root explant is infected by agrobacterium rhizogenes for the hairy root of the explant: taking stem section as explant, pH of culture medium is 5-6, sucrose concentration is 3%, explant age is 30-35D, ultrasonic treatment time is 4s, preculture and co-culture are 2-3D, infection time is 10min, and exogenous hormone is added in 0.1 mg/mLNAA.
According to the technical scheme, the agrobacterium rhizogenes is A4 strain.
According to the technical scheme, the Ri plasmid of the agrobacterium rhizogenes contains an FGF-10 gene, the FGF10 gene is amplified through PCR, and the Ri plasmid is connected after double enzyme digestion.
According to the technical scheme, the astragalus mongholicus hairy roots are subjected to PCR detection: according to the designed FGF-10 gene primer, the same band as the gene primer appears in the band (627 bp), but the untransformed astragalus explant does not amplify the band, so that the Ri plasmid of the agrobacterium is integrated into the genome of the hairy root.
According to the technical scheme, the astragalus mongholicus hairy roots are transferred into a liquid culture medium for amplification and multiplication culture: and (5) culturing the cells by using an MS liquid culture medium, wherein the sucrose concentration is 30g/L, and 30 mu mol/L JA is added for culturing for 30D.
Case 2:
a biological factor eyelash nourishing liquid comprises radix astragali extract, Zingiberis rhizoma extract, vitamin E, glycerol, azone, propylene glycol, sodium hydroxide, and lactic acid.
According to the technical scheme, the biological factor eyelash nourishing liquid comprises, by mass, 4 parts of astragalus extract, 3 parts of dried ginger extract, 0.8 part of vitamin E, 4 parts of glycerol, 0.8 part of azone, 0.9 part of propylene glycol, 0.2 part of sodium hydroxide and 2 parts of lactic acid.
A biological factor eyelash nourishing liquid and a preparation method thereof comprise the following specific steps:
the method comprises the following steps: preparing an astragalus extract: cleaning hairy root of radix astragali, grinding in liquid nitrogen in mortar, adding deionized water, stirring, standing for 5 hr, centrifuging at 8000rpm for 40min, and collecting supernatant to obtain radix astragali extract;
step two: preparing a dried ginger extract: soaking dried ginger powder in 90% ethanol as solvent for 24 hr, slowly percolating at a speed of 1-3 ml per minute, collecting primary percolate 850ml, storing in another container, percolating until the percolate is nearly colorless and the fragrance and pungency of ginger are thin, collecting percolate, concentrating at below 60 deg.C to obtain soft extract, adding primary percolate, mixing, filtering, standing, clarifying, and filtering to obtain ginger fluid extract;
step three: grinding 10% glycerol, adding appropriate amount of purified water, swelling, adding radix astragali extract, Zingiberis rhizoma extract, propylene glycol, azone, propylene glycol, VE, and lactic acid, stirring, and adjusting pH to 5.5-6.5 with sodium hydroxide.
According to the technical scheme, the astragalus extract is extracted from explant hairy roots, and the explant can be roots, stems and leaves.
According to the technical scheme, the astragalus root explant is infected by agrobacterium rhizogenes for the hairy root of the explant: taking stem section as explant, pH of culture medium is 5-6, sucrose concentration is 4%, explant age is 30-35D, ultrasonic treatment time is 4s, preculture and co-culture are carried out for 3D, infection time is 10min, and exogenous hormone is added in an amount of 0.1 mg/mLNAA.
According to the technical scheme, the agrobacterium rhizogenes is 1025 strains.
According to the technical scheme, the Ri plasmid of the agrobacterium rhizogenes contains an FGF-10 gene, the FGF10 gene is amplified through PCR, and the Ri plasmid is connected after double enzyme digestion.
According to the technical scheme, the astragalus mongholicus hairy roots are subjected to PCR detection: according to the designed FGF-10 gene primer, the same band as the gene primer appears in the band (627 bp), but the untransformed astragalus explant does not amplify the band, so that the Ri plasmid of the agrobacterium is integrated into the genome of the hairy root.
According to the technical scheme, the astragalus mongholicus hairy roots are transferred into a liquid culture medium for amplification and multiplication culture: and (5) culturing 35D by adding JA (JA) with 30 mu mol/L into MS liquid culture medium with sucrose concentration of 30 g/L.
In order to detect the effect of the biological factor eyelash nourishing liquid, an in-vitro test is specially carried out:
1. skin irritation test
Multiple applications of mascara solution were used for skin irritation tests. Taking 5 rabbits, depilating with 10% sodium sulfide on both sides of spinal column, each side area is about 40cm 2 After 24 hours, applying eyelash nourishing liquid about 1g to the left depilation area of the rabbit, applying purified water about 1g to the right depilation area, 1 time per day, continuously administering for 7 days, washing out the medicine with warm water after the last application for 24 hours, observing the skin irritation reaction of the rabbit after 1 hour, 24 hours, 48 hours and 72 hours of removing the residual eyelash nourishing liquid, and scoring according to the table 1:
TABLE 1 evaluation index of skin irritation test
Scoring each rabbit according to the scoring principle of table 1, and averaging, the results are shown in table 2:
table 2 skin irritation test results (n =5)
As can be seen from the experimental results, the mascara solution is slightly irritating and causes no edema to the skin after application.
2. Eyelash nourishing liquid in-vitro transdermal experiment
Taking the treated rat skin, fixing the horny layer upwards on Franz diffusion cell of a transdermal apparatus, uniformly coating eyelash nourishing liquid 2g (removing air bubbles) on one side of the horny layer, and the effective diffusion area is 2.48cm 2 7ml of physiological saline containing 20% PEG400 was added to the receiving tank as a receiving solution. Diffusion cellPlacing in a constant temperature water bath at 37 +/-1 ℃ with the stirring speed of 300r/min, sampling 1ml in 1, 2, 4, 6, 8, 10, 12 and 24 hours respectively, and supplementing physiological saline containing 20% of PEG400 at the same temperature and volume. Filtering the sample by using a 0.45 mu m microporous filter membrane, taking 20 mu l of subsequent filtrate for sample introduction, measuring the content, and calculating the accumulated permeability of a unit area in a certain time according to the following formula:
Qs={Cn*Vo+V}/S
wherein, Qs: the unit area accumulated permeation amount during the nth sampling; cn: the concentration in the receiving solution at the time of the nth sampling; vo: a diffusion cell volume; v: sampling volume; s: a diffusion area.
TABLE 3 cumulative Permeability per unit area
The eyelash nourishing liquid has good percutaneous permeability when analyzed by the test result of the visual analysis method. The azone and the propylene glycol play a main role in promoting the penetration of the eyelash nourishing liquid in the process of percutaneous penetration.
3. Experiment for influence of eyelash nourishing liquid on body hair growth of mouse hair removal area
20 Kunming mice are taken, the back hair is cut short, depilating agent (10% NaS) is smeared on the back of the mice for about 2-3 min, the mice are cleaned by warm water, the back of the mice is smooth, no wound or residual hair is removed, and the depilating area is about 3 multiplied by 3cm 2 . The next day, mice were randomly divided into 2 groups of 10 mice each, and blank and eyelash tonic solutions were applied to the depilated area 2 times a day, 0.2 ml/mouse each time, for 15 consecutive days. Taking 10 longest hairs at the same position of a depilating area of each mouse in 16 days, measuring the length of the longest hairs by using a microscope under a microscope, representing the hair length of each mouse by the average length of 10 hairs, and calculating the mean value of the hair lengths of the mice in each group; then, the mice were sacrificed by removing their necks, the furs in the depilated area of the back were cut off, a circular piece of skin was taken out at the same position in the depilated area of each mouse by using a 14mm punch, all the body hairs on the piece of skin were scraped off by a scalpel, and weighed by a ten thousandth balance, and the average hair weight of each group was calculated.
TABLE 4 Effect of eyelash tonic solution on body hair length and weight in mouse depilated area
The experimental results are shown in table 4, compared with the blank matrix group, the hair length and the weight average of the unit body surface area hair of the eyelash nourishing liquid group mice are increased to different degrees, the body hair of the eyelash nourishing liquid group mice is longer and denser than that of the blank matrix group mice, and the eyelash nourishing liquid can promote the body hair regeneration of the chemically unhaired mice.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (1)
1. A biological factor eyelash nourishing liquid is characterized in that: comprises radix astragali extract, Zingiberis rhizoma extract, vitamin E, glycerol, azone, propylene glycol, sodium hydroxide, and lactic acid;
the astragalus extract is extracted from explant hairy roots, wherein the explant is roots, stems and leaves;
the explant hairy roots are infected with the astragalus root explants by agrobacterium rhizogenes: taking a stem section as an explant, wherein the pH value of a culture medium is 5-6, the sucrose concentration is 4%, the age of the explant is 30-35D, the ultrasonic treatment time is 4s, the pre-culture and co-culture are performed for 3D, the infection time is 10min, and exogenous hormone of 0.1mg/mLNAA is added;
the agrobacterium rhizogenes is 1025 strains;
the Ri plasmid of the agrobacterium rhizogenes contains FGF-10 gene, FGF10 gene is amplified through PCR, and the Ri plasmid is connected after double enzyme digestion;
4 parts of astragalus extract, 3 parts of dried ginger extract, 0.8 part of vitamin E, 4 parts of glycerol, 0.8 part of azone, 0.9 part of propylene glycol, 0.2 part of sodium hydroxide and 2 parts of lactic acid according to the mass ratio;
the biological factor eyelash nourishing liquid and the preparation method thereof comprise the following specific steps:
the method comprises the following steps: preparing an astragalus extract: cleaning hairy root of radix astragali, grinding in liquid nitrogen in mortar, adding deionized water, stirring, standing for 5 hr, centrifuging at 8000rpm for 40min, and collecting supernatant to obtain radix astragali extract;
step two: preparing a dried ginger extract: soaking dried ginger powder in 90% ethanol as solvent for 24 hr, slowly percolating at a speed of 1-3 ml per minute, collecting primary percolate 850ml, storing in another container, percolating until the percolate is nearly colorless and the fragrance and pungency of ginger are thin, collecting percolate, concentrating at below 60 deg.C to obtain soft extract, adding primary percolate, mixing, filtering, standing, clarifying, and filtering to obtain ginger fluid extract;
step three: grinding 10% glycerol, adding appropriate amount of purified water, swelling, adding radix astragali extract, Zingiberis rhizoma extract, propylene glycol, azone, propylene glycol, VE, and lactic acid, stirring, and adjusting pH to 5.5-6.5 with sodium hydroxide.
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