CN115025021A - Whitening emulsion containing natural plant extracts - Google Patents
Whitening emulsion containing natural plant extracts Download PDFInfo
- Publication number
- CN115025021A CN115025021A CN202210786491.1A CN202210786491A CN115025021A CN 115025021 A CN115025021 A CN 115025021A CN 202210786491 A CN202210786491 A CN 202210786491A CN 115025021 A CN115025021 A CN 115025021A
- Authority
- CN
- China
- Prior art keywords
- extract
- whitening
- parts
- emulsion containing
- natural plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000002087 whitening effect Effects 0.000 title claims abstract description 77
- 239000000419 plant extract Substances 0.000 title claims abstract description 32
- 239000000839 emulsion Substances 0.000 title claims abstract description 31
- 239000000284 extract Substances 0.000 claims abstract description 55
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- NGSWKAQJJWESNS-UHFFFAOYSA-N 4-coumaric acid Chemical compound OC(=O)C=CC1=CC=C(O)C=C1 NGSWKAQJJWESNS-UHFFFAOYSA-N 0.000 claims abstract description 22
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 21
- 235000020710 ginseng extract Nutrition 0.000 claims abstract description 16
- 244000130592 Hibiscus syriacus Species 0.000 claims abstract description 14
- 235000018081 Hibiscus syriacus Nutrition 0.000 claims abstract description 14
- 241000201920 Salicornia bigelovii Species 0.000 claims abstract description 13
- XTXRWKRVRITETP-UHFFFAOYSA-N Vinyl acetate Chemical compound CC(=O)OC=C XTXRWKRVRITETP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000003906 humectant Substances 0.000 claims abstract description 12
- 235000011187 glycerol Nutrition 0.000 claims abstract description 9
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims description 36
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 33
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 22
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 claims description 22
- 241000208340 Araliaceae Species 0.000 claims description 21
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 21
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 21
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 21
- 235000008434 ginseng Nutrition 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 21
- 238000001704 evaporation Methods 0.000 claims description 19
- 239000007864 aqueous solution Substances 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 15
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 14
- 238000004440 column chromatography Methods 0.000 claims description 13
- 239000000843 powder Substances 0.000 claims description 13
- -1 polypropylene Polymers 0.000 claims description 12
- 229920000936 Agarose Polymers 0.000 claims description 11
- 239000002904 solvent Substances 0.000 claims description 11
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims description 11
- 230000002829 reductive effect Effects 0.000 claims description 10
- 230000005526 G1 to G0 transition Effects 0.000 claims description 9
- 229920002148 Gellan gum Polymers 0.000 claims description 9
- 239000004366 Glucose oxidase Substances 0.000 claims description 9
- 108010015776 Glucose oxidase Proteins 0.000 claims description 9
- 240000000797 Hibiscus cannabinus Species 0.000 claims description 9
- 235000015928 Hibiscus cannabinus Nutrition 0.000 claims description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- 239000004743 Polypropylene Substances 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 9
- XXBDWLFCJWSEKW-UHFFFAOYSA-N dimethylbenzylamine Chemical compound CN(C)CC1=CC=CC=C1 XXBDWLFCJWSEKW-UHFFFAOYSA-N 0.000 claims description 9
- 239000000216 gellan gum Substances 0.000 claims description 9
- 235000010492 gellan gum Nutrition 0.000 claims description 9
- 229940116332 glucose oxidase Drugs 0.000 claims description 9
- 235000019420 glucose oxidase Nutrition 0.000 claims description 9
- 238000000227 grinding Methods 0.000 claims description 9
- 229940046892 lead acetate Drugs 0.000 claims description 9
- 229920001155 polypropylene Polymers 0.000 claims description 9
- 239000011347 resin Substances 0.000 claims description 9
- 229920005989 resin Polymers 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 238000004108 freeze drying Methods 0.000 claims description 8
- 239000000376 reactant Substances 0.000 claims description 8
- 241000201895 Salicornia Species 0.000 claims description 7
- 230000007935 neutral effect Effects 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000010025 steaming Methods 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 4
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 3
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 229940075507 glyceryl monostearate Drugs 0.000 claims description 3
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- 238000001291 vacuum drying Methods 0.000 claims description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 abstract description 20
- 230000000694 effects Effects 0.000 abstract description 16
- 102000003425 Tyrosinase Human genes 0.000 abstract description 15
- 108060008724 Tyrosinase Proteins 0.000 abstract description 15
- 102000016387 Pancreatic elastase Human genes 0.000 abstract description 14
- 108010067372 Pancreatic elastase Proteins 0.000 abstract description 14
- 238000006243 chemical reaction Methods 0.000 abstract description 10
- 230000037394 skin elasticity Effects 0.000 abstract description 6
- 230000001153 anti-wrinkle effect Effects 0.000 abstract description 4
- 230000009467 reduction Effects 0.000 abstract description 4
- 206010002198 Anaphylactic reaction Diseases 0.000 abstract description 3
- 208000003455 anaphylaxis Diseases 0.000 abstract description 3
- 210000003491 skin Anatomy 0.000 description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 8
- 230000008099 melanin synthesis Effects 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 238000002835 absorbance Methods 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 239000002026 chloroform extract Substances 0.000 description 6
- 239000002537 cosmetic Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 241000047339 Salicornia brachystachya Species 0.000 description 5
- 206010040954 Skin wrinkling Diseases 0.000 description 5
- 239000007844 bleaching agent Substances 0.000 description 5
- 239000003054 catalyst Substances 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 229930182494 ginsenoside Natural products 0.000 description 5
- 102000016942 Elastin Human genes 0.000 description 4
- 108010014258 Elastin Proteins 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- AHMIDUVKSGCHAU-LURJTMIESA-N L-dopaquinone Chemical compound [O-]C(=O)[C@@H]([NH3+])CC1=CC(=O)C(=O)C=C1 AHMIDUVKSGCHAU-LURJTMIESA-N 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 229920002549 elastin Polymers 0.000 description 4
- 239000003112 inhibitor Substances 0.000 description 4
- AHMIDUVKSGCHAU-UHFFFAOYSA-N Dopaquinone Natural products OC(=O)C(N)CC1=CC(=O)C(=O)C=C1 AHMIDUVKSGCHAU-UHFFFAOYSA-N 0.000 description 3
- 241000282414 Homo sapiens Species 0.000 description 3
- 206010020751 Hypersensitivity Diseases 0.000 description 3
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 3
- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 3
- 235000002789 Panax ginseng Nutrition 0.000 description 3
- 208000026935 allergic disease Diseases 0.000 description 3
- 230000007815 allergy Effects 0.000 description 3
- 239000011942 biocatalyst Substances 0.000 description 3
- 238000006555 catalytic reaction Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 235000019441 ethanol Nutrition 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 230000037303 wrinkles Effects 0.000 description 3
- 241001061264 Astragalus Species 0.000 description 2
- 241000221377 Auricularia Species 0.000 description 2
- 244000028550 Auricularia auricula Species 0.000 description 2
- 235000000023 Auricularia auricula Nutrition 0.000 description 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- 235000017784 Mespilus germanica Nutrition 0.000 description 2
- 244000182216 Mimusops elengi Species 0.000 description 2
- 235000000560 Mimusops elengi Nutrition 0.000 description 2
- 241000234479 Narcissus Species 0.000 description 2
- 241000304195 Salvia miltiorrhiza Species 0.000 description 2
- 235000011135 Salvia miltiorrhiza Nutrition 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 235000007837 Vangueria infausta Nutrition 0.000 description 2
- 229930003268 Vitamin C Natural products 0.000 description 2
- 125000002252 acyl group Chemical group 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 235000006533 astragalus Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- WGXGKXTZIQFQFO-CMDGGOBGSA-N ethenyl (e)-3-phenylprop-2-enoate Chemical compound C=COC(=O)\C=C\C1=CC=CC=C1 WGXGKXTZIQFQFO-CMDGGOBGSA-N 0.000 description 2
- 125000004494 ethyl ester group Chemical group 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000007760 free radical scavenging Effects 0.000 description 2
- 229940089161 ginsenoside Drugs 0.000 description 2
- 239000002035 hexane extract Substances 0.000 description 2
- 229930005346 hydroxycinnamic acid Natural products 0.000 description 2
- 235000010359 hydroxycinnamic acids Nutrition 0.000 description 2
- 230000033444 hydroxylation Effects 0.000 description 2
- 238000005805 hydroxylation reaction Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 201000001441 melanoma Diseases 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 210000004233 talus Anatomy 0.000 description 2
- NGSWKAQJJWESNS-ZZXKWVIFSA-N trans-4-coumaric acid Chemical compound OC(=O)\C=C\C1=CC=C(O)C=C1 NGSWKAQJJWESNS-ZZXKWVIFSA-N 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- OFUMQWOJBVNKLR-NQQJLSKUSA-N (+)-catechin monohydrate Chemical compound O.C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 OFUMQWOJBVNKLR-NQQJLSKUSA-N 0.000 description 1
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 1
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000871189 Chenopodiaceae Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- UBSCDKPKWHYZNX-UHFFFAOYSA-N Demethoxycapillarisin Natural products C1=CC(O)=CC=C1OC1=CC(=O)C2=C(O)C=C(O)C=C2O1 UBSCDKPKWHYZNX-UHFFFAOYSA-N 0.000 description 1
- 241000218033 Hibiscus Species 0.000 description 1
- 235000005206 Hibiscus Nutrition 0.000 description 1
- 235000007185 Hibiscus lunariifolius Nutrition 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- 241000219071 Malvaceae Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 208000010428 Muscle Weakness Diseases 0.000 description 1
- 206010028372 Muscular weakness Diseases 0.000 description 1
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010029155 Nephropathy toxic Diseases 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 244000071378 Viburnum opulus Species 0.000 description 1
- 235000019013 Viburnum opulus Nutrition 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- HSFWRNGVRCDJHI-UHFFFAOYSA-N alpha-acetylene Natural products C#C HSFWRNGVRCDJHI-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 235000001368 chlorogenic acid Nutrition 0.000 description 1
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 1
- 229940074393 chlorogenic acid Drugs 0.000 description 1
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 1
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 description 1
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000007854 depigmenting agent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000000937 inactivator Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 238000006317 isomerization reaction Methods 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- 235000008777 kaempferol Nutrition 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- 230000003061 melanogenesis Effects 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 1
- 230000007694 nephrotoxicity Effects 0.000 description 1
- 231100000417 nephrotoxicity Toxicity 0.000 description 1
- 201000001119 neuropathy Diseases 0.000 description 1
- 230000007823 neuropathy Effects 0.000 description 1
- 230000037311 normal skin Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 208000033808 peripheral neuropathy Diseases 0.000 description 1
- 230000004526 pharmaceutical effect Effects 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 229920001197 polyacetylene Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 229930004725 sesquiterpene Natural products 0.000 description 1
- 150000004354 sesquiterpene derivatives Chemical class 0.000 description 1
- 230000037384 skin absorption Effects 0.000 description 1
- 231100000274 skin absorption Toxicity 0.000 description 1
- 210000004511 skin melanocyte Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000009044 synergistic interaction Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/368—Carboxylic acids; Salts or anhydrides thereof with carboxyl groups directly bound to carbon atoms of aromatic rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/41—Amines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a whitening emulsion containing natural plant extracts, which consists of the following components: ginseng extract, hibiscus syriacus leaf extract, salicornia bigelovii seed extract, agaric extract, whitening agent, glycerin, humectant and water; the whitening agent is prepared by carrying out a series of reactions on p-hydroxy cinnamic acid and vinyl acetate. Compared with the prior art, the whitening emulsion containing the natural plant extract can effectively penetrate into the horny layer to inhibit tyrosinase activity, reduce the occurrence of anaphylactic reaction, obviously reduce the generation of melanin, improve the whitening effect, reduce the reduction amount of elastase, increase the skin elasticity, improve the anti-wrinkle level and improve the skin state.
Description
Technical Field
The invention relates to the technical field of cosmetics, and particularly relates to a whitening emulsion containing natural plant extracts.
Background
The processing of fine chemicals such as medicines, health products, cosmetics, etc. is rapidly expanding on an industrial level. Tyrosinase is a copper-containing enzyme and plays a key role in the production of melanin, the tyrosinase catalyzes tyrosine into L-3, 4-dihydroxyphenylalanine through hydroxylation and then is oxidized into dopaquinone to produce melanin, the melanin can protect human skin from excessive solar ultraviolet irradiation, and the melanin is a main substance for blackening the skin. Tyrosinase inhibitors of the prior art are currently used for treating epidermal pigmentation, however, the potential adverse effects and low stability of tyrosinase inhibitors remain a serious problem. Moreover, the usual chemical processes for preparing these cosmetics generally require harsh reaction environments and generate large amounts of waste.
The invention patent CN107648131A discloses a whitening skin care product containing natural plant extracts, which consists of a whitening effect component, a matrix component and deionized water, wherein the whitening effect component consists of the following raw materials in parts by weight: 3-10 parts of normal hexane extract of narcissus flower petals, 3-10 parts of chloroform extract of astragalus, 3-10 parts of chloroform extract of salvia miltiorrhiza leaves and 3-10 parts of chloroform extract of medlar. The skin care product has remarkable whitening effect, and has obvious synergistic effect on the aspect of inhibiting the activity of tyrosinase by combining the normal hexane extract of narcissus petals, the chloroform extract of astragalus, the chloroform extract of salvia miltiorrhiza leaves and the chloroform extract of medlar according to a specific proportion, thereby achieving the unexpected whitening effect. The skin care product completely meets indexes in technical specifications for cosmetic safety (2015 edition) issued by the nation, has no irritation to skin, and is safe and reliable. However, the functional substance of the invention adopts plant extract, has the defects of poor stability and low whitening effect, and has the problem of low skin absorption effect.
Disclosure of Invention
In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to provide an emulsion with reduced allergy, natural ingredients, whitening and anti-wrinkle effects.
The plants are good natural sources, have the characteristics of low toxicity and large stock, the functional components of the plants of ginseng, hibiscus syriacus leaves, salicornia bigelovii and agaric are extracted, p-hydroxycinnamic acid and vinyl acetate are added into tetrahydrofuran to react in a packed bed reactor, and the mixture is obtained through reduced pressure evaporation and column chromatography; and then adding the mixture, N-dimethylbenzylamine, agarose, glucose oxidase and gellan gum into toluene, stirring for reaction, dialyzing, and evaporating the solvent to obtain the whitening agent, which can remarkably reduce the generation of melanin, improve the whitening effect, reduce the reduction amount of elastase, increase the skin elasticity and improve the anti-wrinkle level.
In order to achieve the purpose, the invention provides a whitening emulsion containing natural plant extracts, which adopts the following technical scheme:
a whitening emulsion containing natural plant extracts comprises the following components: ginseng extract, hibiscus syriacus leaf extract, salicornia bigelovii seed extract, agaric extract, whitening agent, glycerin, humectant and water.
Preferably, the whitening emulsion containing the natural plant extracts is prepared from the following components in parts by weight: 2-5 parts of black ginseng extract, 4-6 parts of hibiscus syriacus leaf extract, 1-3 parts of salicornia bigelovii extract, 5-8 parts of agaric extract, 5-8 parts of whitening agent, 5-15 parts of glycerin, 6-10 parts of humectant and 60-150 parts of water.
The invention also discloses a preparation method of the whitening emulsion containing the natural plant extract, which comprises the following specific preparation steps:
step 1, mixing water and a ginseng extract, a hibiscus syriacus leaf extract, a salicornia bigelovii extract and an agaric extract, and heating to 70-80 ℃ to obtain a mixed extract;
and 2, cooling the mixed extract prepared in the step 1 to 30-40 ℃, adding a whitening agent, glycerol and a humectant, mixing, homogenizing, and cooling to room temperature to obtain the whitening emulsion containing the natural plant extract.
Preferably, the ginseng extract is prepared by the following method: steaming and boiling the dried ginseng at 90-100 ℃ for 2-5 h, drying at 40-60 ℃ for 20-40 h, and repeating the operation for 5-10 times; and then adding the dried ginseng into 70-85 wt% methanol water solution, adjusting the temperature to 70-90 ℃, grinding for 2-4 h, then evaporating the methanol, recovering the solvent, and freeze-drying to prepare the ginseng extract.
Preferably, the hibiscus cannabinus leaf extract is prepared by the following method: washing the hibiscus cannabinus leaves with water, drying, and then storing overnight at-70 to-90 ℃; and then vacuum drying for 40-60 h, wherein the vacuum degree is 0.0001-0.01 MPa, grinding, and mixing the hibiscus syriacus leaf powder: hexane is 1: 3-6, extracting at 50-70 ℃ for 2-5 h, evaporating to remove hexane, flushing with nitrogen, and freezing for storage to obtain the hibiscus cannabinus leaf extract.
Preferably, the salicornia extract is prepared by the following method: washing Salicornia herbacea with water, and freeze drying; grinding into powder, and mixing the powder with water according to a mass ratio of 1: 20-40, heating at 90-100 ℃ for 0.5-2 h, cooling at room temperature, centrifuging at 8000-12000 rpm for 10-20 min, separating supernatant, and freeze-drying to obtain the salicornia extract.
Preferably, the agaric extract is prepared by the following method: the agaric is washed by water, then dried in the shade at the temperature of 20-35 ℃ for 4-8 days, ground into powder, extracted by 60-80 wt% of ethanol water solution, and the extracting solution is evaporated and dried to obtain the agaric extract.
Preferably, the humectant is a mixture of 1: 1.8-2.5: 0.3-0.8 of glyceryl monostearate, butanediol and triethanolamine.
The p-hydroxycinnamic acid is a potential natural cosmetic, has the capacity of inhibiting melanin generation, has low cytotoxicity and can be well applied to whitening agents. The invention has better biological catalysis efficiency on reactants by carrying out enzymatic reaction in toluene, reduces the influence on the environment and the discharge of wastes in the whole reaction process, and the prepared whitening agent can reduce anaphylactic reaction and has whitening and anti-wrinkle effects.
The preparation steps of the whitening agent are as follows, and the parts are all parts by weight:
s1, dissolving 0.05-0.2 part of p-hydroxy cinnamic acid and 1-2 parts of vinyl acetate in 5-20 parts of tetrahydrofuran, and adding 0.1-0.5 part of 5-15 wt% sulfuric acid aqueous solution to prepare a reactant; reacting for 20-50 min in a packed bed reactor filled with lead acetate, and then transferring to a packed bed reactor filled with polypropylene resin for reacting for 5-20 min; evaporating under reduced pressure, and performing column chromatography on a stationary phase to obtain a mixture;
s2, adding 0.1-0.5 part of N, N-dimethylbenzylamine, 0.01-0.2 part of agarose, 0.01-0.05 part of glucose oxidase and 0.2-1 part of gellan gum into 1-5 parts of toluene, adding the mixture prepared in the step S1, dripping 0.4-1 part of 2-8 wt% sodium hydroxide aqueous solution at a dripping speed of 0.05-0.2 mL/min, stirring and reacting at 20-30 ℃ for 20-40 min, then adding 20-35 wt% hydrochloric acid to adjust the pH value to be neutral, dialyzing and evaporating the solvent to obtain the whitening agent.
Preferably, the stationary phase of the step S1 column chromatography is n-hexane: the mass ratio of ethyl acetate is 7-10: 1 is configured.
In order to improve the stability to hydroxycinnamic acid, the present inventors have conducted intensive immobilization studies and have selected acetaldehyde-agarose as the best immobilizing agent, which we immobilized on acetaldehyde-agarose. The fixing agent not only can obtain higher catalytic efficiency under the condition of low biological catalyst load, but also can be easily mixed with the catalyst in a packed bed reactor, thereby ensuring controllable fluid dynamics and reaction time. Vinyl acetate is a more efficient acyl donor than other ethyl esters in a packed bed reactor mediated catalytic reaction, and thus can be efficiently converted to vinyl cinnamate by first mixing p-hydroxycinnamic acid and vinyl acetate in aqueous tetrahydrofuran and sulfuric acid, and then reacting in a packed bed reactor of lead acetate. By the "trap-release" function of the ion-exchanged polypropylene resin, the unreacted sulfuric acid is recovered, and then the catalyst is easily recovered by column chromatography and can be reused in the system. Because the mixture has poor water solubility, the reaction is carried out in the environment of pure toluene, and because the biocatalyst is efficiently mixed, functional substances are generated by the reaction, and the whitening agent is obtained by dialysis.
Tyrosinase is considered to be a key enzyme in mammalian melanin biosynthesis, and thus inhibition of tyrosinase activity can reduce melanin synthesis. Tyrosinase inhibitors are of various types, such as reducing agents, dopaquinone scavengers, specific tyrosinase inactivators and specific tyrosinase inhibitors. Tyrosinase catalyzes the hydroxylation of tyrosine to generate L-3, 4-dihydroxyphenylalanine, and then the L-3, 4-dihydroxyphenylalanine is oxidized to generate dopaquinone. Melanin is formed by oxidative polymerization of several dopaquinone derivatives. Elastase is primarily associated with the degradation of elastin, a connective tissue component. During the natural aging process or under ultraviolet light conditions, increased elastase activity results in a decrease in elastin, a decrease in skin elasticity, and the appearance of wrinkles. Therefore, inhibition of elastase activity is considered to be an effective measure to prevent skin wrinkling. The whitening agent prepared by the invention acts on skin, can obviously inhibit the activity of tyrosinase, reduces the generation of melanin, and has the whitening effect. But also can act on elastase, reduce the activity of elastase, reduce the reduction amount of elastase, and increase the skin elasticity.
Ginseng is a useful herb for the prevention and treatment of a range of diseases. There are three main types of ginseng: white ginseng, red ginseng and black ginseng. The white ginseng is usually air-dried raw ginseng, and the red ginseng is prepared by steaming raw ginseng at 95-100 ℃ for a period of time. The black ginseng can be extracted from white ginseng, steamed at 95-100 deg.C for 3 hr, circulated for several times, and then dried. The main organic compounds in ginseng include ginsenosides, polysaccharides, sesquiterpenes, polyacetylene, phenolic compounds and others, of which ginsenosides are considered the most important bioactive compounds. Ginsenosides not present in white ginseng. Compared with red ginseng and white ginseng, the biological activity of the black ginseng is obviously improved. During the steaming process, ginsenoside is converted into a less polar component through hydrolysis, isomerization and dehydration. And the ginsenoside has the biological activities of resisting tumor, inflammation, platelet aggregation and the like.
Hibiscus cannabinus is an annual or perennial herb of the genus Hibiscus of the family Malvaceae. Up to 3 m, upright stem, no hair, and sharp prick; leaf allotypes, leaves are rich in bioactive compounds, such as: chlorogenic acid, coffee adjuvant, kaempferol and catechin hydrate. The method is widely applied in the fields of dietary fiber and animal feed. The hibiscus cannabinus leaf extract contains a large amount of polyphenol compounds, has good antioxidant and anti-tyrosinase properties, and has potential to be used as an additional value component for development of cosmetics.
Salicornia herbacea is a whole plant of Salicornia herbacea of Salicornia of Chenopodiaceae, is a green plant growing on beach and saline-alkali beach sand land with stems and leaves, and has a growth period of about 220 days, wherein the green, tender and fresh green stems can be maintained for 50-60 days. Is rich in vitamin C and 18 kinds of amino acids, and is a green health food beneficial to human body. After the seeds are mature, oil can be extracted, and the oil is rich in linoleic acid and is a high-grade raw material for edible and medicinal chemical industry. The medicine has effects of suppressing hyperactive liver, promoting urination, lowering blood pressure, etc.
Auricularia auricula, also called as black fungus and mulberry, is an important edible fungus in China and has wide natural distribution and artificial cultivation. The agaric has soft texture, tender mouthfeel, delicious taste and special flavor, is rich in protein, fat, saccharide, a plurality of vitamins and mineral substances, and has high nutritional value, and each 100 g of the agaric contains 11 g of water, 10.6 g of protein, 0.2 g of fat, 65 g of carbohydrate, 7 g of cellulose, 185 mg of iron, 375 mg of calcium and 201 mg of phosphorus. In addition, it also contains vitamin B, vitamin C, carotene, etc. Therefore, the agaric is a subsidiary food with rich nutrition and delicious taste and has good medical and pharmaceutical effects.
Most of the commonly used skin whitening agents contain harmful chemicals, such as mercury amide or mercury chloride, as active ingredients, and long-term use of such whitening products results in severe systemic reactions, such as allergy, muscle weakness, neuropathy, and nephrotoxicity. On the basis, the functional substance extracted from the plants has the advantages of low allergy and good curative effect.
Compared with the prior art, the invention has the beneficial effects that:
(1) the whitening emulsion containing the natural plant extract can effectively penetrate into the horny layer to inhibit the activity of tyrosinase, reduce the occurrence of anaphylactic reaction and improve the skin state by the synergistic interaction of the components of the black ginseng extract, the hibiscus syriacus leaf extract, the salicornia bigelovii extract and the agaric extract;
(2) adding hydroxycinnamic acid and vinyl acetate into tetrahydrofuran, reacting in a packed bed reactor, decompressing, evaporating, and performing column chromatography to obtain a mixture; and adding the mixture, N-dimethylbenzylamine, agarose, glucose oxidase and gellan gum into toluene, stirring for reaction, and dialyzing to obtain the whitening agent.
Detailed Description
The present invention will be described in further detail with reference to the following specific examples. The procedures, conditions, experimental methods and the like for carrying out the present invention are common knowledge and common general knowledge in the art except for the contents specifically mentioned below, and the present invention is not particularly limited.
For the sake of brevity, the articles used in the following examples are all commercially available products unless otherwise specified, and the methods used are conventional methods unless otherwise specified.
The invention uses part of raw materials with the following sources:
ginseng, derived from Jilin Changbai mountain.
Folium Hibisci, Salicornia bigelovii, derived from Liaoning seed of Viburnum strigosum.
Auricularia auricula from Yunnan Wenshan.
Example 1
A preparation method of whitening emulsion containing natural plant extracts comprises the following components: 3kg of ginseng extract, 5kg of hibiscus syriacus leaf extract, 3kg of salicornia bigelovii extract, 6kg of agaric extract, 7kg of whitening agent, 10kg of glycerin, 8kg of humectant and 100kg of water.
The preparation method of the whitening emulsion containing the natural plant extracts comprises the following steps:
step 1, mixing water and ginseng extract, hibiscus syriacus leaf extract, salicornia bigelovii seed extract and agaric extract, and heating to 75 ℃ to obtain a mixed extract;
and 2, cooling the mixed extract prepared in the step 1 to 35 ℃, adding a whitening agent, glycerol and a humectant, mixing, homogenizing and cooling to room temperature to obtain the whitening emulsion containing the natural plant extract.
The ginseng extract is prepared by the following method: steaming dried Ginseng radix at 95 deg.C for 3 hr, drying at 50 deg.C for 30 hr, and repeating the above steps for 9 times; then adding the dried ginseng into 80wt% methanol water solution, adjusting the temperature to 80 ℃, grinding for 3h, then evaporating the methanol, recovering the solvent, and freeze-drying to prepare the ginseng extract.
The hibiscus cannabinus leaf extract is prepared by the following method: washing folium Hibisci with water, drying, and storing at-80 deg.C overnight; then, the sample is dried in a freeze dryer for 48 hours in vacuum with the vacuum degree of 0.001MPa, and ground, and the weight ratio of the hibiscus syriacus leaf powder: hexane is 1: 5, extracting at 60 deg.C for 3h, evaporating to remove hexane, flushing with nitrogen, and freezing to obtain folium Hibisci extract.
The salicornia extract is prepared by the following method: washing Salicornia herbacea with water, and freeze drying; grinding the dried salicornia into powder by using an electric grinder, and mixing the powder with water according to a mass ratio of 1: 30 mixing, heating at 100 deg.C for 1h, cooling at room temperature, centrifuging at 10000rpm for 15min, separating supernatant, and lyophilizing with lyophilizer to obtain Salicornia Herbacea extract.
The agaric extract is prepared by the following method: washing Auricularia with water, air drying at 30 deg.C in shade for 6 days, grinding into powder, extracting the powder with 70wt% ethanol water solution, and evaporating the extractive solution to obtain Auricularia extract.
The humectant is prepared from the following components in percentage by mass of 1: 2: 0.5 of glyceryl monostearate, butanediol and triethanolamine.
The preparation steps of the whitening agent are as follows:
s1, dissolving 0.1kg of p-hydroxycinnamic acid and 1.6kg of vinyl acetate in 10kg of tetrahydrofuran, and adding 0.2kg of 10wt% sulfuric acid aqueous solution to prepare a reactant; reacting for 30min in a packed bed reactor filled with lead acetate, and then transferring to a packed bed reactor filled with polypropylene resin for reacting for 10 min; evaporated under reduced pressure, purified by n-hexane: the mass ratio of ethyl acetate is 9: 1, carrying out column chromatography on the prepared stationary phase to obtain a mixture;
s2, adding 0.2kg of N, N-dimethylbenzylamine, 0.1kg of agarose, 0.03kg of glucose oxidase and 0.4kg of gellan gum into 2kg of toluene, adding the mixture prepared in the step S1, dropwise adding 0.6kg of 5wt% sodium hydroxide aqueous solution at a dropping speed of 0.1mL/min, stirring and reacting at 28 ℃ for 30min, then adding 30wt% hydrochloric acid to adjust the pH to be neutral, dialyzing, and evaporating to remove the solvent to obtain the whitening agent.
Example 2
A whitening emulsion containing natural plant extracts was prepared in substantially the same manner as in example 1, with the only difference that: the preparation methods of the whitening agents are inconsistent.
The preparation method of the whitening agent comprises the following steps:
s1, dissolving 1.6kg of vinyl acetate in 10kg of tetrahydrofuran, and adding 0.2kg of 10wt% sulfuric acid aqueous solution to prepare a reactant; reacting for 30min in a packed bed reactor filled with lead acetate, and then transferring to a packed bed reactor filled with polypropylene resin for reacting for 10 min; evaporated under reduced pressure, purified by n-hexane: the mass ratio of ethyl acetate is 9: 1, carrying out column chromatography on the prepared stationary phase to obtain a mixture;
s2, adding 0.2kg of N, N-dimethylbenzylamine, 0.1kg of agarose, 0.03kg of glucose oxidase and 0.4kg of gellan gum into 2kg of toluene, adding the mixture prepared in the step S1, dropwise adding 0.6kg of 5wt% sodium hydroxide aqueous solution at a dropping speed of 0.1mL/min, stirring and reacting at 28 ℃ for 30min, then adding 30wt% hydrochloric acid to adjust the pH to be neutral, dialyzing, and evaporating to remove the solvent to obtain the whitening agent.
Example 3
A whitening emulsion containing natural plant extracts was prepared in substantially the same manner as in example 1, with the only difference that: the preparation methods of the whitening agents are inconsistent.
The preparation method of the whitening agent comprises the following steps:
s1, dissolving 0.1kg of p-hydroxycinnamic acid in 10kg of tetrahydrofuran, and adding 0.2kg of 10wt% sulfuric acid aqueous solution to prepare a reactant; reacting for 30min in a packed bed reactor filled with lead acetate, and then transferring to a packed bed reactor filled with polypropylene resin for reacting for 10 min; evaporated under reduced pressure, purified by n-hexane: the mass ratio of ethyl acetate is 9: 1, carrying out column chromatography on the prepared stationary phase to obtain a mixture;
s2, adding 0.2kg of N, N-dimethylbenzylamine, 0.1kg of agarose, 0.03kg of glucose oxidase and 0.4kg of gellan gum into 2kg of toluene, adding the mixture prepared in the step S1, dropwise adding 0.6kg of 5wt% sodium hydroxide aqueous solution at a dropping speed of 0.1mL/min, stirring and reacting at 28 ℃ for 30min, then adding 30wt% hydrochloric acid to adjust the pH to be neutral, dialyzing, and evaporating to remove the solvent to obtain the whitening agent.
Example 4
A whitening emulsion containing natural plant extracts was prepared in substantially the same manner as in example 1, with the only difference that: the preparation methods of the whitening agents are inconsistent.
The preparation method of the whitening agent comprises the following steps:
s1, dissolving 0.1kg of p-hydroxycinnamic acid and 1.6kg of vinyl acetate in 10kg of tetrahydrofuran, and adding 0.2kg of 10wt% sulfuric acid aqueous solution to prepare a reactant; reacting for 30min in a packed bed reactor filled with lead acetate, and then transferring to a packed bed reactor filled with polypropylene resin for reacting for 10 min; evaporated under reduced pressure, purified by n-hexane: the mass ratio of ethyl acetate is 9: 1, carrying out column chromatography on the prepared stationary phase to obtain a mixture;
s2, adding 0.2kg of N, N-dimethylbenzylamine, 0.03kg of glucose oxidase and 0.4kg of gellan gum into 2kg of toluene, adding the mixture prepared in the step S1, dripping 0.6kg of 5wt% sodium hydroxide aqueous solution at a dripping speed of 0.1mL/min, stirring and reacting at 28 ℃ for 30min, adding 30wt% hydrochloric acid to adjust the pH value to be neutral, dialyzing, and evaporating to remove the solvent to obtain the whitening agent.
Comparative example 1
A whitening emulsion containing natural plant extracts was prepared in substantially the same manner as in example 1, with the only difference that: the preparation methods of the whitening agents are inconsistent.
The preparation method of the whitening agent comprises the following steps:
s1, adding 0.2kg of 10wt% sulfuric acid aqueous solution into 10kg of tetrahydrofuran to prepare a reactant; reacting for 30min in a packed bed reactor filled with lead acetate, and then transferring to a packed bed reactor filled with polypropylene resin for reacting for 10 min; evaporated under reduced pressure, purified by n-hexane: the mass ratio of ethyl acetate is 9: 1, carrying out column chromatography on the prepared stationary phase to obtain a mixture;
s2, adding 0.2kg of N, N-dimethylbenzylamine, 0.03kg of glucose oxidase and 0.4kg of gellan gum into 2kg of toluene, adding the mixture prepared in the step S1, dripping 0.6kg of 5wt% sodium hydroxide aqueous solution at a dripping speed of 0.1mL/min, stirring and reacting at 28 ℃ for 30min, adding 30wt% hydrochloric acid to adjust the pH value to be neutral, dialyzing, and evaporating to remove the solvent to obtain the whitening agent.
Comparative example 2
A whitening emulsion containing natural plant extracts was prepared in substantially the same manner as in example 1, with the only difference that: replacing the whitening agent with ascorbic acid.
Ascorbic acid, which can affect melanogenesis by reducing reactive oxygen levels in melanocytes, is a commonly used whitening agent.
Test example 1
Melanin synthesis inhibition test:
cultured melanoma cells of passage 4 at 1X 10 4 The seeds are inoculated on a 6-well plate at a density of one/mL, and the solution is changed after 24 hours. The samples prepared in examples and comparative examples were added, and each sample was added to 3 wellsThe sample concentration was 100. mu.L per well. The control was added with 0.9% aqueous sodium chloride solution instead of the sample. At 37 deg.C, 5% CO 2 After 72h incubation at concentration, the supernatant was discarded. 1mL of 2.5g/L pancreatin was added to each well and digested at room temperature for 5 min. Digestion was stopped by adding 4mL of culture medium and pipetting to a single cell suspension. Taking 0.5mL as cell count, centrifuging the rest cell suspension at 1500r/min for 10min, discarding the supernatant, adding 1mL of 1mol/L sodium hydroxide aqueous solution, incubating in 90 ℃ water bath for 2h, and measuring the absorbance value at 490nm of an enzyme labeling instrument. The melanin synthesis amount and the inhibition rate of the drug on the melanin synthesis are calculated according to the following formulas.
Melanin synthesis inhibition ═ 1- (sample well absorbance value/sample well cell density)/(control well absorbance value/control well cell density)) × 100%
The results are shown in table 1:
the basic structure of the melanoma cell line, particularly the melanin synthesis function, is basically consistent with that of normal skin melanocytes of human beings, and it can be seen from table 1 that the sample of example 1 has the highest melanin synthesis inhibition rate, probably because the acetaldehyde-agarose is used as the best fixing agent in the invention, and p-hydroxycinnamic acid is fixed on the acetaldehyde-agarose, and the fixing agent not only can obtain higher catalytic efficiency under the condition of low biocatalyst loading, but also can be easily doped with a catalyst in a packed bed reactor, thereby ensuring controllable fluid dynamics and reaction time. Vinyl acetate is a more efficient acyl donor than other ethyl esters in a packed bed reactor mediated catalytic reaction, and thus can be efficiently converted to vinyl cinnamate by first mixing p-hydroxycinnamic acid and vinyl acetate in aqueous tetrahydrofuran and sulfuric acid, and then reacting in a packed bed reactor of lead acetate. By the "trap-release" function of the ion-exchanged polypropylene resin, the unreacted sulfuric acid is recovered, and then the catalyst is easily recovered by column chromatography and can be reused in the system. Because the mixture has poor water solubility, the reaction is carried out in the environment of pure toluene, and because the biocatalyst is efficiently mixed, functional substances are generated by the reaction, and the whitening agent is obtained by dialysis. The whitening agent acts on skin, can obviously inhibit the activity of tyrosinase, reduces the generation of melanin, and has whitening effect.
Test example 2
Free radical clearance test:
weighing 20mg of 2, 2-biphenyl-1-picrylhydrazino, and using 95wt% of absolute ethyl alcohol to fix the volume to 250mL for later use; 20mg of the samples prepared in examples and comparative examples were made up to 250mL with 99.9wt% ethanol aqueous solution for further use.
The testing step comprises the steps of taking 2mL of prepared sample and 2mL of 2, 2-biphenyl-1-picrylhydrazino solution, placing the sample and the 2, 2-biphenyl-1-picrylhydrazino solution in a test tube with a plug, placing the test tube in a thermostat, keeping the temperature for 1h at 37 ℃, taking 99.9wt% ethanol water solution as a blank control, and measuring the absorbance A of each sample to be tested at the wavelength of 517nm in an ultraviolet spectrophotometer i And the clearance is calculated as follows: clearance = [1- (a) i -A j )/A c ]×100%
A c The absorbance is 2mL of 99.9wt% ethanol aqueous solution added with 2mL of 2, 2-biphenyl-1-picrylhydrazino solution;
A i adding 2mL of 2, 2-biphenyl-1-picrylhydrazino solution into 2mL of prepared sample to obtain absorbance;
A j the absorbance of 2mL of a prepared sample added with 2mL of 99.9wt% ethanol aqueous solution;
three groups were tested for each sample, and the tests were averaged and the results are shown in table 2:
in general, collagen in the skin reacts by the action of free radicals, so that the skin does not receive sufficient nutrition supply, thus causing the skin tissue to lose its vitality, lose its elasticity, generate wrinkles, dull skin color, etc., and the free radical scavenging ability reflects the antioxidant ability of the sample, and as can be seen from the experimental data of table 2, the sample prepared in example 1 has the best free radical scavenging ability, probably because elastase is mainly involved in the degradation of elastin, which is a connective tissue component. During the natural aging process or under ultraviolet light conditions, increased elastase activity results in a decrease in elastin, a decrease in skin elasticity, and the appearance of wrinkles. Therefore, inhibition of elastase activity is considered to be an effective measure to prevent skin wrinkling. The complete whitening agent prepared in embodiment 1 of the invention has good oxidation resistance, can act on elastase, reduces the activity of elastase, reduces the reduction amount of elastase, and increases skin elasticity.
Claims (10)
1. A whitening emulsion containing natural plant extracts is characterized by comprising the following components: ginseng extract, hibiscus syriacus leaf extract, salicornia bigelovii seed extract, agaric extract, whitening agent, glycerin, humectant and water.
2. The whitening emulsion containing natural plant extracts as claimed in claim 1, which is composed of the following components in parts by weight: 2-5 parts of black ginseng extract, 4-6 parts of hibiscus syriacus leaf extract, 1-3 parts of salicornia bigelovii extract, 5-8 parts of agaric extract, 5-8 parts of whitening agent, 5-15 parts of glycerin, 6-10 parts of humectant and 60-150 parts of water.
3. The whitening emulsion containing natural plant extracts according to claim 1 or 2, wherein the ginseng extract is prepared by the following method: steaming and boiling the dried ginseng at 90-100 ℃ for 2-5 h, drying at 40-60 ℃ for 20-40 h, and repeating the operation for 5-10 times; and then adding the dried ginseng into 70-85 wt% methanol water solution, adjusting the temperature to 70-90 ℃, grinding for 2-4 h, then evaporating the methanol, recovering the solvent, and freeze-drying to prepare the ginseng extract.
4. The whitening emulsion containing natural plant extracts of claim 1 or 2, wherein the hibiscus cannabinus leaf extract is prepared by the following method: washing the hibiscus cannabinus leaves with water, drying, and then, preserving overnight at the temperature of minus 70 to minus 90 ℃; and then vacuum drying for 40-60 h, wherein the vacuum degree is 0.0001-0.01 MPa, grinding, and mixing the hibiscus syriacus leaf powder: hexane is 1: 3-6, extracting at 50-70 ℃ for 2-5 h, evaporating to remove hexane, flushing with nitrogen, and freezing for storage to obtain the hibiscus cannabinus leaf extract.
5. The whitening emulsion containing natural plant extracts according to claim 1 or 2, wherein the salicornia extract is prepared by the following method: washing Salicornia bigelovii Torr with water, and freeze drying; grinding into powder, and mixing the powder with water according to a mass ratio of 1: 20-40, heating at 90-100 ℃ for 0.5-2 h, cooling at room temperature, centrifuging at 8000-12000 rpm for 10-20 min, separating supernatant, and freeze-drying to obtain the salicornia extract.
6. The whitening emulsion containing natural plant extracts according to claim 1 or 2, wherein the agaric extract is prepared by the following method: the agaric is washed by water, then dried in the shade at the temperature of 20-35 ℃ for 4-8 days, ground into powder, extracted by 60-80 wt% of ethanol water solution, and the extracting solution is evaporated and dried to obtain the agaric extract.
7. The whitening emulsion containing natural plant extracts of claim 1 or 2, wherein the humectant is selected from the group consisting of 1: 1.8-2.5: 0.3-0.8 of glyceryl monostearate, butanediol and triethanolamine.
8. The whitening emulsion containing natural plant extracts as claimed in claim 1 or 2, wherein the preparation steps of the whitening agent are as follows, the parts are all parts by weight:
s1, dissolving 0.05-0.2 part of p-hydroxycinnamic acid and 1-2 parts of vinyl acetate in 5-20 parts of tetrahydrofuran, and adding 0.1-0.5 part of 5-15 wt% sulfuric acid aqueous solution to prepare a reactant; reacting for 20-50 min in a packed bed reactor filled with lead acetate, and then transferring to a packed bed reactor filled with polypropylene resin for reacting for 5-20 min; evaporating under reduced pressure, and performing column chromatography on a stationary phase to obtain a mixture;
s2, adding 0.1-0.5 part of N, N-dimethylbenzylamine, 0.01-0.2 part of agarose, 0.01-0.05 part of glucose oxidase and 0.2-1 part of gellan gum into 1-5 parts of toluene, adding the mixture prepared in the step S1, dripping 0.4-1 part of 2-8 wt% sodium hydroxide aqueous solution at a dripping speed of 0.05-0.2 mL/min, stirring and reacting at 20-30 ℃ for 20-40 min, adding 20-35 wt% hydrochloric acid to adjust the pH value to be neutral, and dialyzing; evaporating under reduced pressure to remove solvent to obtain whitening agent.
9. The whitening emulsion containing natural plant extracts of claim 7, wherein: the stationary phase of the column chromatography in the step S1 is normal hexane and ethyl acetate according to the mass ratio of 7-10: 1 is configured.
10. The whitening emulsion containing natural plant extracts as claimed in claim 1 or 2, which is prepared by the following steps:
step 1, mixing water and a ginseng extract, a hibiscus syriacus leaf extract, a salicornia bigelovii extract and an agaric extract, and heating to 70-80 ℃ to obtain a mixed extract;
and 2, cooling the mixed extract prepared in the step 1 to 30-40 ℃, adding a whitening agent, glycerol and a humectant, mixing, homogenizing and cooling to room temperature to obtain the whitening emulsion containing the natural plant extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210786491.1A CN115025021B (en) | 2022-07-06 | 2022-07-06 | Whitening emulsion containing natural plant extracts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210786491.1A CN115025021B (en) | 2022-07-06 | 2022-07-06 | Whitening emulsion containing natural plant extracts |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115025021A true CN115025021A (en) | 2022-09-09 |
| CN115025021B CN115025021B (en) | 2023-10-27 |
Family
ID=83129032
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210786491.1A Active CN115025021B (en) | 2022-07-06 | 2022-07-06 | Whitening emulsion containing natural plant extracts |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115025021B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105997628A (en) * | 2016-05-21 | 2016-10-12 | 广州丹奇日用化工厂有限公司 | Moisturizing and anti-aging cosmetic containing agaric extract and preparation method of cosmetic |
| CN108033881A (en) * | 2017-11-30 | 2018-05-15 | 五邑大学 | A kind of tyrosinase inhibitor in half-natural source and preparation method and application |
| CN108354873A (en) * | 2018-05-22 | 2018-08-03 | 佛山云裳化妆品有限公司 | One kind is dispelled scar composition and its application |
| US20190290575A1 (en) * | 2018-03-23 | 2019-09-26 | Mary Kay Inc. | Topical compositions and methods |
| CN114159364A (en) * | 2021-12-24 | 2022-03-11 | 大通汉麻生物科技研究院(青岛)有限公司 | Cosmetic composition containing hibiscus syriacus stem powder and preparation method thereof |
-
2022
- 2022-07-06 CN CN202210786491.1A patent/CN115025021B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105997628A (en) * | 2016-05-21 | 2016-10-12 | 广州丹奇日用化工厂有限公司 | Moisturizing and anti-aging cosmetic containing agaric extract and preparation method of cosmetic |
| CN108033881A (en) * | 2017-11-30 | 2018-05-15 | 五邑大学 | A kind of tyrosinase inhibitor in half-natural source and preparation method and application |
| US20190290575A1 (en) * | 2018-03-23 | 2019-09-26 | Mary Kay Inc. | Topical compositions and methods |
| CN108354873A (en) * | 2018-05-22 | 2018-08-03 | 佛山云裳化妆品有限公司 | One kind is dispelled scar composition and its application |
| CN114159364A (en) * | 2021-12-24 | 2022-03-11 | 大通汉麻生物科技研究院(青岛)有限公司 | Cosmetic composition containing hibiscus syriacus stem powder and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 徐广华等, vol. 1, 中国环境科学出版社, pages: 155 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115025021B (en) | 2023-10-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR100997441B1 (en) | Method for preparing high purity ginsenoside rd using lactobacillus casei and cosmetic composition for anti-wrinkle comprising the high purity ginsenoside rd | |
| KR101041403B1 (en) | Cosmetic Composition for Anti-oxidation and Whitening | |
| KR20210114893A (en) | Cosmetic and OTC drug composition comprising peach fermented extract as an active ingredient | |
| KR101936294B1 (en) | Composition comprising for skin-whitening and anti-wrinkling extract of Rumex acetosella L. or extract of Hydrangea serrata | |
| KR20130090150A (en) | Antioxidizing functional cosmetic compositions for containing extracted/fermented dendropanax morbifera products and functional cosmetics produced thereby | |
| KR101981016B1 (en) | Functional moisturizing, antioxidant and wrinkle-improving functional cosmetic composition and preparing method thereof | |
| KR101189838B1 (en) | Composition for improving condition of hair and scalp containing fermented plant extracts | |
| KR20130001842A (en) | Cosmetic composition and makeup method for the skin whitening comprising the styrax obassia extracts | |
| JP2023171950A (en) | Anti-aging agent, antioxidant, anti-inflammatory agent, and whitening agent, as well as cosmetic | |
| KR102051939B1 (en) | Manufacturing method and cosmetic ingredient using Dendropanax morbifera Lev extraction fluid | |
| CN115025021B (en) | Whitening emulsion containing natural plant extracts | |
| CN110577937A (en) | SOD complex enzyme for removing freckles and beautifying skin and preparation method thereof | |
| KR101960571B1 (en) | Method for preparing apricot extract, the apricot extract prepared therefrom, and skin care or cosmetic composition comprising the apricot extract | |
| KR102288902B1 (en) | Cosmetic composition for anti-wrinkle and enhancing elasticity comprising fermented extract of Phragmites Communis | |
| KR100466379B1 (en) | Composition for skin whitening | |
| KR20130136753A (en) | Cosmetic composition comprising cercis chinensis or reynoutria japonica for. elata extract for skin whitening | |
| KR20120118946A (en) | Skin whitening complex containing trihydroxyisoflavone and glycyrrhiza uralensis extracts | |
| KR20120007170A (en) | Method for fermenting broussonetia plant or the extract thereof, the fermented extracts thereby and the cosmetic composition containing the same | |
| CN114939093B (en) | Whitening and moisturizing composition containing amino acid and application of composition in cosmetics | |
| KR100510104B1 (en) | Fermentative Extract of Rooibos and Cosmetic Compositions Comprising the Same for Preventing Skin Aging | |
| CN115368334B (en) | Method for improving extraction efficiency of total flavonoids of bamboo leaves | |
| KR102615332B1 (en) | Cosmetic composition comprising Feverfew extract | |
| KR101945527B1 (en) | The Cosmetic Composition Including The Sap Of Juglans mandshurica Maxim. As An Active Ingredient | |
| KR20230148576A (en) | Cosmetic composition comprising fermented green tea | |
| KR101207562B1 (en) | Cosmetic composition comprising the extract of Cotinus coggygria as active Ingredient |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20230825 Address after: No. n817, 3rd floor, xingguangyingjing, No. 117, Shuiyin Road, Yuexiu District, Guangzhou, Guangdong 510000 Applicant after: Zhiyueyun (Guangzhou) Digital Information Technology Co.,Ltd. Address before: No. 152, Xingang West Road, Haizhu District, Guangzhou, Guangdong 510000 Applicant before: Guangdong Industry Technical College Effective date of registration: 20230825 Address after: Room 1801, No. 17, Zhujiang West Road, Tianhe District, Guangzhou, Guangdong 510000 (office only) Applicant after: GUANGZHOU FANZHIRONG COSMETICS Co.,Ltd. Applicant after: Guangzhou Qingnong Biotechnology Co.,Ltd. Address before: No. n817, 3rd floor, xingguangyingjing, No. 117, Shuiyin Road, Yuexiu District, Guangzhou, Guangdong 510000 Applicant before: Zhiyueyun (Guangzhou) Digital Information Technology Co.,Ltd. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |