CN115024251A - Rapid acquisition method for full-male Pseudobagrus ussuriensis - Google Patents
Rapid acquisition method for full-male Pseudobagrus ussuriensis Download PDFInfo
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- CN115024251A CN115024251A CN202210778113.9A CN202210778113A CN115024251A CN 115024251 A CN115024251 A CN 115024251A CN 202210778113 A CN202210778113 A CN 202210778113A CN 115024251 A CN115024251 A CN 115024251A
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- 241001663722 Tachysurus ussuriensis Species 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000003098 androgen Substances 0.000 claims abstract description 65
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 46
- 241000251468 Actinopterygii Species 0.000 claims abstract description 43
- 239000011259 mixed solution Substances 0.000 claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- GCKMFJBGXUYNAG-UHFFFAOYSA-N 17alpha-methyltestosterone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C)(O)C1(C)CC2 GCKMFJBGXUYNAG-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229940122815 Aromatase inhibitor Drugs 0.000 claims abstract description 15
- GCKMFJBGXUYNAG-HLXURNFRSA-N Methyltestosterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@](C)(O)[C@@]1(C)CC2 GCKMFJBGXUYNAG-HLXURNFRSA-N 0.000 claims abstract description 15
- 239000003886 aromatase inhibitor Substances 0.000 claims abstract description 15
- 229960001566 methyltestosterone Drugs 0.000 claims abstract description 15
- 230000012447 hatching Effects 0.000 claims abstract description 11
- 235000016709 nutrition Nutrition 0.000 claims abstract description 10
- 230000035764 nutrition Effects 0.000 claims abstract description 10
- 238000003287 bathing Methods 0.000 claims abstract description 9
- 241000595940 Notostraca Species 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 16
- 150000001875 compounds Chemical class 0.000 claims description 14
- 230000000366 juvenile effect Effects 0.000 claims description 13
- 239000000243 solution Substances 0.000 claims description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 230000032678 sex differentiation Effects 0.000 claims description 3
- 238000007598 dipping method Methods 0.000 claims description 2
- 238000012423 maintenance Methods 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims 2
- 238000011534 incubation Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 230000006872 improvement Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- 229940088597 hormone Drugs 0.000 description 7
- 239000005556 hormone Substances 0.000 description 7
- 210000004907 gland Anatomy 0.000 description 6
- 210000002149 gonad Anatomy 0.000 description 6
- 230000006698 induction Effects 0.000 description 5
- 238000002224 dissection Methods 0.000 description 4
- 238000007667 floating Methods 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 231100001076 gonad atrophy Toxicity 0.000 description 2
- 206010003694 Atrophy Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003163 gonadal steroid hormone Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000001776 parthenogenetic effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000008142 sex development Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/184—Hormones
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Polymers & Plastics (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Marine Sciences & Fisheries (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
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Abstract
The invention relates to the technical field of manual control of other people in aquaculture, and discloses a rapid acquisition method of pseudobagrus ussuriensis, which comprises the following steps: selecting fish fries, wherein the fish fries are pseudobagrus ussuriensis fries hatched from 5 to 6 ten days of the year, and enter a mixed nutrition stage at 3 days after hatching; preparing androgen mixed solution, namely mixing methyltestosterone dissolved by ethanol and an aromatase inhibitor; androgen treatment, feeding with bait treated by the androgen mixed solution, and bathing with the androgen mixed solution with low concentration; maintaining the treatment condition, controlling the water temperature at 28-30 deg.C, and maintaining for 65-75 days. The method can efficiently obtain the all-male pseudobagrus ussuriensis fries for breeding production, greatly shortens the time required for obtaining the all-male fries by the traditional method, effectively reduces the labor intensity, improves the working efficiency, and powerfully promotes the improvement of the breeding yield and the economic benefit of the pseudobagrus ussuriensis.
Description
Technical Field
The invention relates to the technical field of manual control of other people in aquaculture, in particular to a rapid acquisition method of pseudobagrus ussuriensis.
Background
The sex of the fish has strong plasticity, and the growth difference between the sexes can be fully utilized through the artificial control of the sex of the fish, so that the breeding purpose of breeding the all-male or all-female high-yield strain is achieved. Researchers research in various fishes and find that sex hormone induction has important influence on fish sex development, genetic sex of the fishes can be changed by hormone induction before sex differentiation of the fishes, so that sex reversion is generated, and some fishes can be subjected to sex reversion under the action of hormone even when the fishes are mature. Therefore, hormone induction is an important means for obtaining a parthenogenetic population in fish, but the sensitivity of different fish to different hormones varies greatly, and the induction effect varies.
Pseudobagrus ussuriensis (Pseudobagrus ussuriensis)Pseudobagrus ussuriensis) Is a famous and excellent cultured fish with obvious growth advantageThe male type (the weight of the male can reach more than 3 times of that of the female of the same age), has wide market prospect due to tender meat, no muscle prick, rich nutrition and delicious taste, is deeply loved by consumers, and is bred in more than ten provinces nationwide at present. In view of the great growth advantage of pseudobagrus ussuriensis in male, if full-male cultivation can be realized, the improvement of the cultivation yield of the pseudobagrus ussuriensis can be greatly promoted.
Disclosure of Invention
The purpose of the invention is as follows: aiming at the problems in the prior art, the invention provides a rapid acquisition method for pseudobagrus ussuriensis, which can realize the rapid acquisition of pseudobagrus ussuriensis and powerfully promote the improvement of the pseudobagrus ussuriensis culture yield.
The technical scheme is as follows: the invention provides a rapid acquisition method of pseudobagrus ussuriensis, which comprises the following steps: selecting fish fries, wherein the fish fries are pseudobagrus ussuriensis fries hatched from 5 to 6 ten days of the year, and enter a mixed nutrition stage at 3 days after hatching; preparing androgen mixed solution, namely mixing methyltestosterone dissolved by ethanol and an aromatase inhibitor; androgen treatment, feeding with bait treated by the androgen mixed solution, and dipping with the low-concentration androgen mixed solution; maintaining the treatment condition, controlling the water temperature at 28-30 deg.C, and maintaining for 65-75 days.
Preferably, in the fry selecting step, the fry must be selected for fry that have not been subjected to a sex differentiation stage.
Preferably, the fry is fry which starts to be taken from the outside immediately 3 days after hatching and is in a mixed nutrition stage.
Preferably, in the step of preparing the androgen mixture, the mass ratio of methyltestosterone to aromatase inhibitor in the androgen mixture is 1: 2.
Preferably, in the step of preparing the androgen mixture solution, the specific method is as follows: respectively dissolving 60-120 mg of methyltestosterone and 120-240 mg of aromatase inhibitor in a certain volume of 95% ethanol, and then mixing according to equal volume to prepare the compound.
Preferably, in the step of androgen treatment, bait feeding after treatment with the androgen mixture solution comprises two stages: the feeding stage of the fairy shrimp live baits treated by the androgen mixed liquor and the feeding stage of the compound feed treated by the androgen mixed liquor.
Preferably, in the feeding stage of the live fairy shrimp bait treated by the androgen mixed solution, the method for treating fairy shrimp by the androgen mixed solution is that the androgen mixed solution is added into the fairy shrimp hatching water body according to the volume fraction of 100-.
Preferably, in the feeding stage of the compound feed treated by the androgen mixed solution, according to the proportion of 60-120 mg of methyltestosterone and 120-240 mg of aromatase inhibitor in each kilogram of feed, the androgen mixed solution and the feed are mixed uniformly and dried at low temperature for feeding, and the fish fry is fed for 3-4 times every day on the 35-75 days after the fish fry are hatched.
Preferably, in the androgen treatment step, a specific method of using the androgen mixture solution immersion bath at a low concentration is: and adding the androgen mixed solution into a fry culture tank according to the volume fraction of 100-200 mu L/L after the fry is hatched 15-35 days so that the fry is in a bathing environment.
Preferably, in the step of maintaining the treatment conditions, the water temperature of the culture tank is controlled at 28-30 ℃ through an intelligent automatic temperature control system, the water temperature is checked at least 3 times every day, and the dissolved oxygen is controlled to be more than 5-8 mg/L and is maintained for 65-75 days.
Has the advantages that: the method adopts the androgen mixed solution direct induction technology to realize the production of the all-male pseudobagrus ussuriensis, greatly shortens the culture period of the all-male fishes, effectively reduces the labor intensity and improves the working efficiency compared with the traditional genetic operation means.
In the invention, the androgen treatment adopts a technical mode of combining feeding, bathing and high-temperature cultivation environment control, so that the technical defect of a single treatment mode can be effectively overcome, and the full male colony is ensured to be obtained.
In conclusion, the rapid acquisition method for the all-male pseudobagrus ussuriensis can realize the efficient acquisition of the all-male pseudobagrus ussuriensis, greatly shortens the time required by the traditional genetic operation technology for acquiring all-male groups, obviously reduces the labor intensity, improves the working efficiency and has a powerful promoting effect on the improvement of the pseudobagrus ussuriensis culture yield.
Detailed Description
The present invention will now be described in detail with reference to specific embodiments.
Embodiment 1:
a rapid acquisition method for pseudobagrus ussuriensis all-male includes the following steps:
step one, fry selection: the pseudobagrus ussuriensis young fish which is hatched in 25 days in 5 months in 2020 is raised in a culture tank with 100L water body, and when the fish fry reaches 28 days in 5 months, the fish fry starts to eat in an opening and enters a mixed nutrition stage.
Step two, preparing androgen mixed liquor: dissolving 65 mg of methyltestosterone into 200 mL of 95% ethanol, dissolving 130 mg of aromatase inhibitor into 200 mL of 95% ethanol, and mixing 200 mL of ethanol solutions of the two hormones to prepare an androgen mixed solution with the mass ratio of the methyltestosterone to the aromatase inhibitor being 1: 2.
Step three, androgen treatment: adding 150 mu L of the androgen mixed solution prepared in the second step into 1L of fairy shrimp hatching water, feeding the filtered fairy shrimp to juvenile fish after the fairy shrimp is hatched for 12 h, respectively feeding the juvenile fish in the morning, the noon and the evening every day, and feeding the juvenile fish from the 3 rd day to the 35 th day after the fry is hatched, wherein the 15 th to 35 th days, adding 10 mL of the androgen mixed solution prepared in the second step into a culture tank to ensure that the fry is in a bathing environment; after 35 days, 400 mL of the androgen mixed solution prepared in the second step is mixed with 1 kg of floating compound feed and stirred sufficiently and uniformly, ethanol is volatilized by a low-temperature air dryer, and the mixture is fed with the treated feed from 36 th to 65 th at the frequency of 4 times per day.
Step four, maintaining the treatment conditions: the water temperature in the culture tank is controlled at 28-30 ℃ by an intelligent automatic temperature control system, the dissolved oxygen is controlled at more than 5-8 mg/L, androgen treatment is not carried out after 65 days of maintenance, and normal compound feed is used for feeding instead. On the 65 th day, 100 individuals are randomly selected, sex is judged by observing the gonads through a stereoscopic microscope after dissection, 93 individuals are found to be spermary gonads, 5 individuals cannot judge sex due to gonad atrophy, and 2 individuals are facultative gonads, so that the pseudobagrus ussuriensis production efficiency of the embodiment is 93%.
Embodiment 2:
a rapid acquisition method for pseudobagrus ussuriensis all-male includes the following steps:
step one, fry selection: the pseudobagrus ussuriensis young fish which is hatched in 25 days in 5 months in 2020 is raised in a culture tank with 100L water body, and when the fish fry reaches 28 days in 5 months, the fish fry starts to eat in an opening and enters a mixed nutrition stage.
Step two, preparing androgen mixed liquor: respectively dissolving 75 mg of methyltestosterone into 200 mL of 95% ethanol, dissolving 150 mg of aromatase inhibitor into 200 mL of 95% ethanol, and mixing 200 mL of ethanol solutions of the two hormones to prepare an androgen mixed solution with the mass ratio of the methyltestosterone to the aromatase inhibitor being 1: 2.
Step three, androgen treatment: adding 160 mu L of the androgen mixed solution prepared in the second step into 1L of fairy shrimp hatching water, feeding the filtered fairy shrimp to juvenile fish after the fairy shrimp is hatched for 12 h, respectively feeding the juvenile fish in the morning, the noon and the evening once a day, and feeding the juvenile fish from the 3 rd day to the 35 th day after the fry is hatched, wherein the 15 th to 35 th days, adding 12 mL of the androgen mixed solution prepared in the second step into a culture tank to ensure that the fry is in a bathing environment; after 35 days, 400 mL of the androgen mixture prepared in the second step is mixed with 1 kg of floating compound feed and stirred sufficiently and uniformly, ethanol is volatilized by a low-temperature air dryer, and the mixture is fed with the treated feed from 36 th to 75 th days according to the frequency of feeding 4 times per day.
Step four, maintaining the treatment conditions: the water temperature in the culture tank is controlled at 28-30 ℃ by an intelligent automatic temperature control system, the dissolved oxygen is controlled at more than 5-8 mg/L, and the androgen treatment is not carried out after the culture tank is maintained for 75 days, and the culture is carried out by using normal compound feed instead. On 75 th day, 100 individuals are randomly selected, the sex is judged by observing the gonads through a stereoscopic microscope after dissection, 95 individuals with gonads as spermary, 1 individual with gonad atrophy and no judgment on sex are found, and 4 individuals with facultative gonads, so that the pseudobagrus ussuriensis production efficiency of the embodiment is 95%.
Embodiment 3:
a rapid acquisition method for pseudobagrus ussuriensis all-male includes the following steps:
step one, fry selection: the pseudobagrus ussuriensis young fish which is hatched in 25 days in 5 months in 2020 is raised in a culture tank with 100L water body, and when the fish fry reaches 28 days in 5 months, the fish fry starts to eat in an opening and enters a mixed nutrition stage.
Step two, preparing androgen mixed liquor: respectively dissolving 80 mg of methyltestosterone into 200 mL of 95% ethanol, dissolving 160 mg of aromatase inhibitor into 200 mL of 95% ethanol, and mixing 200 mL of ethanol solutions of the two hormones to prepare an androgen mixed solution with the mass ratio of the methyltestosterone to the aromatase inhibitor being 1: 2.
Step three, androgen treatment: adding 165 mu L of the androgen mixed liquor prepared in the second step into 1L of fairy shrimp hatching water, after the fairy shrimp hatches for 15h, feeding the filtered fairy shrimp to juvenile fish, respectively feeding the juvenile fish in the morning, the noon and the evening once a day, and feeding the juvenile fish from the 3 rd day to the 35 th day after the fry hatches, wherein 15 mL of the androgen mixed liquor prepared in the second step is added into a culture tank for keeping the fry in a bathing environment for 15 to 35 days; after 35 days, 400 mL of the androgen mixture prepared in the second step is mixed with 1 kg of floating compound feed and stirred sufficiently and uniformly, ethanol is volatilized by a low-temperature air dryer, and the mixture is fed with the treated feed from 36 th to 75 th days according to the frequency of feeding 4 times per day.
Step four, maintaining the treatment conditions: the water temperature in the culture tank is controlled at 28-30 ℃ by an intelligent automatic temperature control system, the dissolved oxygen is controlled at more than 5-8 mg/L, and the androgen treatment is not carried out after the culture tank is maintained for 75 days, and the culture is carried out by using normal compound feed instead. On 75 th day, 100 individuals are randomly selected, the sex glands are observed by using a stereoscopic microscope after dissection to judge the sex, the sex glands of 92 individuals are found to be spermary, the sex gland atrophy of 3 individuals cannot be judged to be the sex, and the sex gland of 5 individuals is facultative, so that the pseudobagrus ussuriensis production efficiency is 92%. Comparative example:
a rapid acquisition method for pseudobagrus ussuriensis all-male includes the following steps:
step one, fry selection: the pseudobagrus ussuriensis young fish which is hatched in 25 days in 5 months in 2020 is raised in a culture tank with 100L water body, and when the fish fry reaches 28 days in 5 months, the fish fry starts to eat in an opening and enters a mixed nutrition stage.
Step two, 400 mL of 95% ethanol was prepared.
Step three, test treatment: adding 165 mu L of the ethanol obtained in the second step into 1L of fairy shrimp hatching water, after the fairy shrimp hatches for 15h, feeding the filtered fairy shrimp to juvenile fish, respectively feeding the juvenile fish in the morning, the noon and the evening once a day, and feeding the juvenile fish from 3 days to 35 days after the fry hatches, wherein 15 mL of the ethanol obtained in the second step is added into a culture tank to enable the fry to be in a bathing environment after 15-35 days; after 35 days, 400 mL of the ethanol obtained in the second step was mixed with 1 kg of the floating compound feed and sufficiently stirred, the ethanol was volatilized by a low-temperature air dryer, and the feed was fed with the treated feed from 36 th to 75 th days at a frequency of 4 times per day.
Step four, maintaining the treatment conditions: the water temperature in the culture tank is controlled at 28-30 ℃ by an intelligent automatic temperature control system, the dissolved oxygen is controlled at more than 5-8 mg/L, ethanol treatment is not carried out after the water temperature is maintained for 75 days, and normal compound feed is used for feeding instead. On 75 days, 100 individuals are randomly selected, sex glands are observed by using a stereo microscope after dissection to judge the sex, and the sex glands of 52 individuals are found to be spermary and 48 individuals are found to be ovary, so that the production efficiency of the pseudobagrus ussuriensis male in the comparative example is 52%.
The above embodiments are merely illustrative of the technical concepts and features of the present invention, and the purpose of the embodiments is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (10)
1. A rapid acquisition method for pseudobagrus ussuriensis is characterized by comprising the following steps:
selecting fries, wherein the fries are pseudobagrus ussuriensis juvenile fishes hatched from middle ten days to 6 months of each year, and enter a mixed nutrition stage on the 3 rd day after hatching;
preparing androgen mixed solution, namely mixing methyltestosterone dissolved by ethanol and an aromatase inhibitor;
androgen treatment, feeding with bait treated by the androgen mixed solution, and bathing with the low-concentration androgen mixed solution;
the treatment condition is maintained, the water temperature is controlled at 28-30 ℃, and the treatment condition is maintained for 65-75 days.
2. The rapid acquisition method of pseudobagrus ussuriensis according to claim 1, wherein said fry selection step is performed by selecting fry which have not been subjected to a sex differentiation stage.
3. The rapid acquisition method of pseudobagrus ussuriensis according to claim 2, wherein said fries are fries which start to be taken from the outside immediately 3 days after hatching and are in a mixed nutrition phase.
4. A rapid acquisition method of pseudobagrus ussuriensis according to claim 1, wherein the weight ratio of methyltestosterone to aromatase inhibitor in said androgen mixture is 1:2 in said preparation step of said androgen mixture.
5. The rapid full pseudobagrus ussuriensis acquisition method according to claim 1, wherein the preparation of said androgen mixture is carried out by the following steps:
respectively dissolving 60-120 mg of methyltestosterone and 120-240 mg of aromatase inhibitor in a certain volume of 95% ethanol, and then mixing according to equal volume to prepare the compound.
6. A rapid acquisition method of pseudobagrus ussuriensis as claimed in claim 1, wherein said step of androgen treatment, bait feeding after treatment with said androgen mixture comprises two phases: the feeding stage of the fairy shrimp live baits treated by the androgen mixed liquor and the feeding stage of the compound feed treated by the androgen mixed liquor.
7. The method for rapidly acquiring Pseudobagrus ussuriensis's whole male Pseudobagrus ussuriensis' as claimed in claim 6, wherein in the feeding stage of said androgen mixed solution treatment for live bait, the method for treating Pseudobagrus ussuriensis's by androgen mixed solution is that said androgen mixed solution is added into the incubation water of Pseudobagrus ussuriensis's in a volume fraction of 100-.
8. The rapid acquisition method for Pseudobagrus ussuriensis as claimed in claim 6, wherein in the feeding stage of the androgen mixed solution treatment compound feed, the mixture of androgen and feed is mixed and stirred evenly and air-dried at low temperature according to the proportion of methyltestosterone 60-120 mg and aromatase inhibitor 120-240 mg per kg of feed, and the feed is fed 3-4 times a day 35-75 days after fry hatching.
9. A rapid acquisition method of pseudobagrus ussuriensis as claimed in claim 1, wherein said androgen treatment step, the dipping with said androgen mixture solution at low concentration is carried out by: and adding the androgen mixed solution into a fry culture tank according to the volume fraction of 100-200 mu L/L after the fry is hatched 15-35 days so that the fry is in a bathing environment.
10. The rapid acquisition method for pseudobagrus ussuriensis according to any one of claims 1 to 9, wherein in the step of maintaining the processing conditions, the water temperature in the culture tank is controlled at 28-30 ℃ by an intelligent automatic temperature control system, the water temperature is checked at least 3 times a day, the dissolved oxygen is controlled at 5-8 mg/L or more, and the maintenance time is 65-75 days.
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