CN115006465B - Flushing fluid for treating animal urinary calculus and preparation method thereof - Google Patents

Abstract

The application provides a flushing fluid for treating animal urinary calculi and a preparation method thereof, and relates to the field of animal medical treatment. The flushing liquid for treating animal urinary stones comprises the following components in every 100 mL: 1mL-5mL of cordate houttuynia distillate, 1mL-5mL of bupleurum extract, 0.5g-5g of sodium hyaluronate, 1g-10g of acidifying buffer and the balance of water for injection. The preparation method of the flushing liquid comprises the following steps: adding an acidification buffer into water for injection to obtain a buffer solution; adding cordate houttuynia distillate, bupleurum root extract and sodium hyaluronate into the buffer solution to obtain a mixed solution, and adjusting the pH value of the mixed solution to obtain the flushing liquid for treating animal urinary stones. The flushing fluid for treating animal urinary calculus plays a good role in treating urinary calculus in an animal urinary system, and has the characteristics of high cure rate, low recurrence rate and low irritation.

Description

Flushing fluid for treating animal urinary calculus and preparation method thereof

Technical Field

The invention relates to the field of animal medical treatment, in particular to a flushing fluid for treating animal urinary calculi and a preparation method thereof.

Background

Urinary calculus, also called urinary calculus, refers to a urinary organ disease in which inorganic salt (or organic) crystalline coagulum in the urinary tract stimulates urinary mucosa to cause bleeding, inflammation and obstruction, is a generic term for kidney calculus, ureteral calculus, vesical calculus and urinary calculus, and is clinically characterized by dysuria, pain in the obstructed part and hematuria. Urinary stones can cause obstruction of ureter, bladder and urethra, cause damage to kidney tissues and kidney functions, and further cause kidney failure. In recent years, the incidence rate of urinary stones in pets is in an annual rising trend, and the quality of life is seriously influenced.

According to the clinical characteristics of urination disorders such as frequent urination, painful urination and the like, such as hematuria and the like, the position and the quantity of the stones can be determined by combining the diagnosis and palpation as well as the X-ray examination and the ultrasonic exploration. After the diagnosis of urinary calculus, the treatment method is to remove the urinary calculus, diminish inflammation of urinary tract, support therapy and other measures. Surgery is a means for removing urinary calculus, but surgery has a large damage to animals, and if the operation is not thorough in stone removal, surgery therapy is a cause of urinary calculus recurrence. Conservative therapies such as urinary tract irrigation are often used in the treatment of pet stones. In general, normal saline is used as a flushing agent for flushing the urinary tract, a catheter is inserted into the urinary tract, a large amount of normal saline is injected into the catheter, and when the pressure reaches a certain level, the catheter is suddenly released and simultaneously pressed on the abdomen to flush out small stones, and the flushing operation can be repeated for several times until no foreign matters exist in the flushing liquid. The method is simple to operate and low in cost, but the recurrence rate of urinary calculus is still high after the physiological saline washing treatment. The appearance of urolithiasis prescription food can certainly play a certain role in preventing urolithiasis recurrence, but the food therapy has long litholytic time and low efficiency. Therefore, it is necessary to find a washing liquid capable of dissolving stones to reduce the recurrence rate of urinary stones and effectively cure urinary system diseases such as urinary crystallization, urinary stones and the like.

Disclosure of Invention

The invention aims to provide a flushing fluid for treating animal urinary stones and a preparation method thereof, wherein the flushing fluid has the effects of small irritation, dissolving crystallization stones, repairing bladder urethra mucous membrane, diminishing inflammation and relieving pain, and animals subjected to flushing treatment are not easy to relapse, so that urinary system diseases such as animal urinary crystallization, urinary stones and the like can be effectively cured.

In order to achieve the above object, the technical scheme of the present application is as follows:

a rinse solution for treating urinary stones in an animal, wherein each 100mL of the rinse solution comprises: 1mL-5mL of cordate houttuynia distillate, 1mL-5mL of bupleurum extract, 0.5g-5g of sodium hyaluronate, 1g-10g of acidifying buffer and the balance of water for injection.

Preferably, each 100mL of the rinse solution comprises: 3mL-5mL of cordate houttuynia distillate, 3mL-5mL of bupleurum extract, 3g-5g of sodium hyaluronate, 1g-5g of acidifying buffer and the balance of water for injection.

Preferably, the acidifying buffer comprises any one of acetic acid-acetate, citric acid-citrate, and phosphoric acid-phosphate.

Preferably, the acidifying buffer is acetic acid-sodium acetate;

the acetic acid is 0.5g-5g in each 100mL of the flushing liquid, and the sodium acetate is 0.5g-5g in each 100mL of the flushing liquid.

Preferably, the animal comprises any one of a cat, a dog, a pig.

Preferably, the urinary calculi include magnesium ammonium phosphate calculi and calcium phosphate calculi.

The application also provides a preparation method of the flushing fluid for treating animal urolithiasis, which comprises the following steps: adding the acidification buffer into water for injection to obtain a buffer solution;

adding the cordate houttuynia distilled liquid, the bupleurum root extracting solution and the sodium hyaluronate into the buffer solution to obtain a mixed solution;

and adjusting the pH value of the mixed solution to obtain the flushing liquid for treating animal urinary stones.

Preferably, the temperature of the water for injection is 75-85 ℃.

Preferably, the pH of the mixed solution is 4.2-4.8.

Preferably, after adjusting the pH, the method further comprises the steps of filling, capping and sterilizing the mixed solution.

The beneficial effects of this application:

the acidic solution is formed by using the acidic buffer, so that the acidic solution can effectively dissolve urethra and alkaline crystals and stones in the urinary bladder, has low irritation to urinary bladder mucous membrane, is matched with the anti-inflammatory and antibacterial effects of the houttuynia cordata extract, has the antipyretic and analgesic effects of the bupleurum, has the effects of repairing the damage of the urinary bladder and urinary tract mucous membrane, has good comprehensive treatment effect on urinary stone diseases in the urinary system of animals by combining the medicines, and has the characteristics of high cure rate, low recurrence rate and low irritation.

The preparation method of the flushing fluid for treating animal urinary stones has the advantages of simple process, low manufacturing cost and high operability, and is beneficial to mass production and preparation.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope of the present invention.

FIG. 1 is a microscopic image of the product of comparative example 5 after testing;

FIG. 2 is a microscopic image of the product of comparative example 6 after testing;

FIG. 3 is a microscopic image of the rinse solution prepared in example 1 after the test.

Detailed Description

The term as used herein:

"prepared from … …" is synonymous with "comprising". The terms "comprising," "including," "having," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, step, method, article, or apparatus.

The conjunction "consisting of … …" excludes any unspecified element, step or component. If used in a claim, such phrase will cause the claim to be closed, such that it does not include materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of … …" appears in a clause of the claim body, rather than immediately following the subject, it is limited to only the elements described in that clause; other elements are not excluded from the stated claims as a whole.

When an equivalent, concentration, or other value or parameter is expressed as a range, preferred range, or a range bounded by a list of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when ranges of "1 to 5" are disclosed, the described ranges should be construed to include ranges of "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a numerical range is described herein, unless otherwise indicated, the range is intended to include its endpoints and all integers and fractions within the range.

In these examples, the parts and percentages are by mass unless otherwise indicated.

"parts by mass" means a basic unit of measurement showing the mass ratio of a plurality of components, and 1 part may be any unit mass, for example, 1g may be expressed, 2.689g may be expressed, and the like. If we say that the mass part of the a component is a part and the mass part of the B component is B part, the ratio a of the mass of the a component to the mass of the B component is represented as: b. alternatively, the mass of the A component is aK, and the mass of the B component is bK (K is an arbitrary number and represents a multiple factor). It is not misunderstood that the sum of the parts by mass of all the components is not limited to 100 parts, unlike the parts by mass.

"and/or" is used to indicate that one or both of the illustrated cases may occur, e.g., a and/or B include (a and B) and (a or B).

The present application provides a rinse solution for treating animal urolithiasis, wherein each 100mL of the rinse solution comprises: the houttuynia cordata distillate may be 1mL-5mL, for example, 1mL, 1.5mL, 2mL, 2.5mL, 3mL, 3.5mL, 4mL, 4.5mL, 5mL or 1mL-5mL, the bupleurum extract may be 1mL-5mL, for example, 1mL, 1.5mL, 2mL, 2.5mL, 3mL, 3.5mL, 4mL, 4.5mL, 5mL or 1mL-5mL, the sodium hyaluronate may be 0.5g-5g, for example, 0.5g, 1g, 1.5g, 2g, 2.5g, 3g, 3.5g, 4g, 4.5g, 5g or 0.5g-5g, the acidifying buffer may be 1g-10g, for example, 1g, 2g, 3g, 4g, 5g, 6g, 7g, 8g, 9g, 10g or 1g-10g, the balance of water may be used for injection.

The cordate houttuynia has the effects of clearing heat and detoxicating, and particularly has obvious antibacterial effect on various bacteria, fungi and the like after distillation and extraction, and also has obvious anti-inflammatory effect. The distilled extract is poured into organs such as animal kidney, so that capillary vessel can be dilated, blood flow and urine secretion can be increased, and the animal kidney has good diuretic effect.

Bupleuri radix and its exterior and interior relieving effects can be used for treating common cold with fever, chest and hypochondrium distending pain, etc., and has effects of relieving fever and pain. After the bupleurum is extracted, the volatile oil, saikosaponin and other components in the bupleurum extract can relieve fever and resist inflammation, the bupleurum polysaccharide and other components can enhance immunity, and the extracted components have good antibacterial and antiviral effects on the urinary tract.

The sodium hyaluronate can treat various pathogenic factors in the urinary system, and the disease treating factors easily cause bladder mucous membrane defect or bladder mucous membrane surface coating deficiency, so the sodium hyaluronate can promote the diseases to heal as soon as possible, thereby achieving the purpose of repairing.

The acidic buffer is usually an acidic buffer material which has small stimulation to the urinary system, and can chemically dissolve alkaline stones through an acidic environment, so that the effects of treating urinary crystallization and stones are realized. The water for injection is water without any additive, can ensure that the flushing liquid does not contain virus, bacteria, pyrogen and other impurities, and can well dissolve other additives.

Through the compounding of the substances, an acidic litholytic solution is obtained, and the alkaline litholytic solution can promote the dissolution of alkaline litholysis during perfusion and irrigation, protect the mucous membrane of the bladder and the urethra, control the bacterial infection of the urinary tract, and especially can relieve the pain caused by the litholysis by using the chemical litholytic mode.

In an alternative embodiment of the present application, each 100mL of the rinse solution comprises: 3mL-5mL of cordate houttuynia distillate, 3mL-5mL of bupleurum extract, 3g-5g of sodium hyaluronate, 1g-5g of acidifying buffer and the balance of water for injection.

Preferably, each 100mL of the rinse solution comprises: 5mL of cordate houttuynia distillate, 5mL of bupleurum extract, 5g of sodium hyaluronate, 2.25g of acidifying buffer and the balance of water for injection.

In an alternative embodiment of the present application, the acidifying buffer comprises any one of acetic acid-acetate, citric acid-citrate, and phosphoric acid-phosphate.

Preferably, the acidifying buffer may be selected from acetic acid-sodium acetate buffer substances.

Wherein acetic acid is 0.5g-5g per 100mL of the rinse solution, and may be, for example, any of 0.5g, 1g, 1.5g, 2g, 2.5g, 3g, 3.5g, 4g, 4.5g, 5g, or 0.5g-5g, and sodium acetate is 0.5g-5g per 100mL of the rinse solution, and may be, for example, any of 0.5g, 1g, 1.5g, 2g, 2.5g, 3g, 3.5g, 4g, 4.5g, 5g, or 0.5g-5g.

More preferably, acetic acid is 1.09g per 100mL of the rinse solution and sodium acetate is 1.16g per 100mL of the rinse solution.

Animals that can be treated with the rinse solution of the present application include essentially any of cats, dogs, and pigs, and in particular can be used to treat urolithiasis in pet cats and dogs.

The flushing liquid is an acidic litholytic liquid, can be used for perfusing and flushing the bladder or urethra, and can achieve the purpose of litholysis more directly. Thus, the treatment of urinary calculi herein is primarily directed to basic calculi, including magnesium ammonium phosphate and calcium phosphate calculi.

The application also provides a preparation method of the flushing fluid for treating animal urolithiasis, which comprises the following steps: adding the acidification buffer into water for injection to obtain a buffer solution; and continuously adding the cordate houttuynia distillate, the bupleurum extracting solution and the sodium hyaluronate to obtain a mixed solution, and adjusting the pH value of the mixed solution to obtain the flushing liquid for treating animal urinary stones.

In an alternative embodiment of the present application, the water for injection has a temperature of 75 ℃ to 85 ℃, for example, can be 75 ℃, 78 ℃, 80 ℃, 82 ℃, 85 ℃, or any value from 75 ℃ to 85 ℃.

Preferably, the temperature of the water for injection is selected to be 80 ℃, so that when the cordate houttuynia distillate, the bupleurum root extracting solution and the sodium hyaluronate are added into the buffer solution, the dissolution rate of the components can be greatly accelerated, and the mixed solution can be quickly prepared.

It should be noted that, in general, the cordate houttuynia distillate, the bupleurum extract, the sodium hyaluronate and the like need to be sequentially added to the buffer solution, and if these substances are simultaneously added to the buffer solution, the obtained mixed solution is easily turbid.

In an alternative embodiment of the present application, it is also desirable to readjust the pH of the solution to any value from 4.2 to 4.8, such as 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8 or 4.2 to 4.8, after the mixed solution is obtained.

Preferably, the pH is adjusted to 4.5, which is primarily to ensure that the alkaline urolithiasis is promoted to dissolve when a subsequent irrigation rinse is performed. If the pH of the mixed solution is not in the range of 4.2 to 4.8, an appropriate amount of acetic acid or sodium acetate may be added for adjustment.

In an alternative embodiment of the present application, after adjusting the pH of the mixed solution, further comprising potting, capping, and sterilizing the mixed solution. Specifically, a 10mL tube bottle can be selected for filling, sealing, capping and then autoclaving.

Embodiments of the present invention will be described in detail below with reference to specific examples, but it will be understood by those skilled in the art that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.

Experimental medicine: the cordate houttuynia distillate used in the embodiment of the application is prepared by a laboratory, and the specific preparation method comprises the following steps: weighing 100g of dried houttuynia cordata, pulverizing into fine powder, adding 500mL of deionized water, placing into a distiller, heating, collecting distilled products, repeating distillation once again, and mixing the two products to obtain houttuynia cordata distillate; the bupleurum extracting solution is prepared by a laboratory, and the specific preparation method comprises the following steps: pulverizing 100g of radix bupleuri into fine powder, adding 800g of 85% ethanol, reflux-extracting at 85deg.C for 3 times, mixing the three reflux solutions, and recovering ethanol under reduced pressure until no ethanol smell exists to obtain radix bupleuri extractive solution; sodium hyaluronate was purchased from chengdou koryoon industrial and trade company, inc; acetic acid was purchased from Chengdu Keli's industry and trade company, inc.; sodium acetate is purchased from Chengdu Keli's industry and trade company, inc.

Example 1

In this example, each 100mL of rinse solution for treating animal urolithiasis includes: 5mL of cordate houttuynia distillate, 5mL of bupleurum extract, 5g of sodium hyaluronate, 1.09g of acetic acid, 1.16g of sodium acetate and the balance of water for injection.

The preparation method comprises the following steps: heating 85mL of water for injection to 80 ℃, adding 1.09g of acetic acid and 1.16g of sodium acetate, stirring until all the materials are dissolved, sequentially adding 5mL of cordate houttuynia distillate, 5mL of bupleurum root extract and 5g of sodium hyaluronate, stirring uniformly, then fixing the volume of the mixed solution to 100mL, detecting the pH value of the mixed solution to 4.5, and then carrying out encapsulation, sterilization and the like to obtain a finished product of the flushing fluid for treating animal urolithiasis.

Example 2

The rinse solution for treating animal urolithiasis of every 100mL of this example comprises, in addition to water for injection: 5mL of cordate houttuynia distillate, 5mL of bupleurum extract, 5g of sodium hyaluronate and an acidification buffer. The acidifying buffer is sodium phosphate-phosphate. The method for preparing the rinse solution was the same as in example 1, and the amount of the acidifying buffer was mainly such that the pH of the final rinse solution was 4.5.

Example 3

This example is the same as example 2 except that the acidifying buffer is citric acid-sodium citrate.

Comparative example 1

In this comparative example, only acetic acid-sodium acetate acidifying buffer was used for each 100mL of rinse solution for treating animal urinary stones, except for water for injection, and the pH of the entire rinse solution was 4.5.

Comparative example 2

In this comparative example, 5mL of houttuynia cordata distillate and an acidulated buffer of acetic acid-sodium acetate were added to 100mL of the rinse for treating animal urolithiasis, in addition to water for injection, and the pH of the entire rinse was 4.5.

Comparative example 3

In this comparative example, 5mL of bupleurum extract and an acidulated buffer of acetic acid-sodium acetate were added to 100mL of the rinse for treating animal urolithiasis, in addition to water for injection, and the pH of the entire rinse was 4.5.

Comparative example 4

In the rinsing liquid for treating animal urinary stones per 100mL of the comparative example, in addition to the water for injection, 5mL of houttuynia cordata distillate, 5mL of bupleurum extract and an acidulated buffer of acetic acid-sodium acetate were added, and the pH of the whole rinsing liquid was 4.5.

Comparative example 5

The rinse solution of this comparative example was purchased from an existing rinse solution product on the market under the brand name Renacidin.

Comparative example 6

The rinse solution of this comparative example is also an existing rinse solution product on the market, and the product brand is sub G.

Evaluation of the effects of different types of buffer urinary tract washes

To examine the litholytic effect of different types of acidic buffers at the same pH and the irritation test on animals, the urethral irrigation treatment was performed on 18 cases of naturally occurring cat urethral crystallization by preparing an acetic acid-sodium acetate buffer, a phosphoric acid-sodium phosphate buffer, and a citric acid-sodium citrate buffer with water for injection, respectively, at pH 4.5, and evaluating the litholytic effect. The litholytic effect is classified into three grades of good grade, general grade and poor grade according to the urethra flushing frequency of the pet doctor, and the irritation is classified into three grades of large irritation, general irritation and small irritation according to the animal stress degree, and the statistical results are shown in table 1.

TABLE 1 evaluation of urethral flushing Effect of different types of acidic buffers

Group of	Stone dissolving effect	Irritation (irritation)
			Acetic acid-sodium acetate buffer	Good quality	In general
Phosphate-sodium phosphate buffer	Difference of difference	In general
			Citric acid-sodium citrate buffer	Good quality	Big size

As can be seen from table 1, the combined use effect of the acidification buffer of acetic acid-sodium acetate is better than the use effect of the phosphate buffer and the citrate buffer. That is, acetic acid-sodium acetate is preferred for use herein as the acidifying buffer in the rinse solution.

Evaluation of in vivo use Effect of acidic buffer without pH

To examine the litholytic effect of acidic buffers of different pH values and the irritation test on animals, acidic buffers of acetic acid-sodium acetate having ph=3.6, ph=4.5, and ph=6.0 were prepared, respectively, and urethral irrigation treatment was performed on 18 cases of naturally occurring cat urethral crystallization, and litholytic effect and irritation were evaluated. The litholytic effect is classified into three grades according to the good, general and differential grades mainly according to the urethra flushing times of the pet doctor, the irritation is classified into three grades of large irritation, general irritation and small irritation according to the animal stress degree, and the statistical results are shown in table 2.

TABLE 2 evaluation of urethral flushing Effect of acid buffers of different pH values

Group of	Stone dissolving effect	Irritation (irritation)
			pH＝3.6	Good quality	Big size
pH＝4.5	Good quality	In general
			pH＝6.0	In general	In general

As is clear from Table 2, the effect of the acid buffer having pH 4.5 was superior to the effect of the acid buffer having pH 3.6 and pH 6.0.

Clinical treatment effect evaluation of flushing liquid with different formulas:

the present application conducted research experiments on the different formulations of example 1 and comparative examples 1-4. Three key indexes of cure time, irritation and litholytic effect are adopted for analysis and statistics, and each index is specifically divided into three grades for integration and statistics: treatment time: prolonged (0 score), normal (1 score), shortened (2 score); irritation: big (0 min), general (1 min), small (2 min); stone dissolving effect: difference (0 min), general (1 min), good (2 min). Cats with natural onset and urethral crystallization cases were treated and counted to determine the therapeutic use of different formulations, and the results are shown in table 3.

Table 3 urinary tract rinse effect score for example 1 and comparative examples 1-4

Group of	Number of cases	Total score	Average score
				Comparative example 1	15	10	0.67
Comparative example 2	12	11	0.91
				Comparative example 3	16	14	0.88
Comparative example 4	15	18	1.2
				Example 1	21	32	1.52

As can be seen from Table 3, the formulation of acetic acid-sodium acetate buffer+houttuynia cordata+bupleurum chinense+sodium hyaluronate in example 1 of the present application has the highest score, and is superior to the other formulations of comparative examples 1 to 4 in terms of use effect.

Test of irritation of flushing fluid to mucous membrane

The 24 healthy dogs were equally divided into 3 groups and bladder perfusion was performed. The first group selected the product of comparative example 5 under the brand Renacidin, the second group selected the product of comparative example 6 under the brand sub G, and the third group selected the rinse solution prepared in example 1 of the present application.

After each group of dogs was anesthetized, the urinary bladder was drained with a catheter, and then 20ml of rinse solution was slowly injected. Bladder mucosal tissue was taken from each group of surgery 5 hours after perfusion, 10% formalin fixed, paraffin sections prepared according to conventional procedures, HE stained, and microscopic examination.

The first set of perfused rinse solutions, the results after microscopy are shown in fig. 1, the second set of microscopy results are shown in fig. 2, and the third set of microscopy results are shown in fig. 3. As can be seen from the figure: the first group and the second group have obvious mucosal blood vessel congestion, the third group has slight congestion, and the first group and the second group have unsmooth mucosal layers, which indicates that flushing liquid has certain damage to the mucosa, and the third group has smooth mucosal layers. This demonstrates that the irritancy of the irrigation solution prepared in this application to the bladder mucosa is less than that of the products of comparative examples 5 and 6.

In vitro alkaline litholytic efficacy test

The specific experimental method is as follows: 30 phosphate stones were selected, all from the same clinical case, and were divided into three groups of 10 each, with insignificant initial weight differences. The first group selected the product of comparative example 5 under the brand Renacidin, the second group selected the product of comparative example 6 under the brand sub G, and the third group selected the rinse solution prepared in example 1 of the present application.

According to the grouping, adding corresponding litholytic liquid into each group of litholytic bottles, lightly shaking the bottles to enable the stones to be fully contacted with the litholytic liquid, placing the litholytic bottles in a constant temperature incubator at 37 ℃ to dissolve the stones, taking out each group of stones after 12 hours, placing the stones in an oven for drying and weighing, recording the weight of the stones, and counting the weight reduction rate of the urinary stones of each group, wherein the formula is as follows: (weight before stone dissolution-weight after stone dissolution)/weight before stone dissolution) ×100%.

The test results are shown in Table 4, and it is evident that each test group has a better in vitro litholytic effect, but the effect of the present application is best, and the difference is significant compared with the two groups of comparative example 5 and comparative example 6.

TABLE 4 in vitro litholytic efficacy of each test group

". Times." indicates significant differences

The flushing fluid prepared by the method also carries out clinical curative effect tests, and mainly aims at the clinical curative effect tests of the magnesium ammonium phosphate urolithiasis of the pet cat.

And (3) case selection: 42 pet cats were collected in 5 pet hospitals in the city of capital, and were clinically diagnosed as cases of incomplete obstruction of urinary tract, and urinary stones were detected by urinary sediment as magnesium ammonium phosphate as the main component. These cases were divided into 2 groups of 21 cases each, and a comparative treatment trial was performed. The treatment group was subjected to flushing treatment with the flushing liquid of example 1 of the present application, and the control group was subjected to flushing treatment with physiological saline.

The treatment method comprises the following steps: all treatment groups and control groups adopt the consistent treatment mode, take anti-inflammation, anti-emetic, pain relieving, fluid supplementing and potassium reducing as treatment principles, correct electrolyte disturbance, reduce stress sources, regulate the emotion of the suffering cats, and feed cat food with balanced nutrition every day. In the treatment, after the urine in the bladder is drained, the cat is anesthetized and then the catheter is inserted into the urethra for treatment. The treatment status of different groups of pet cats is recorded, and periodic re-diagnosis is performed after the treatment is finished.

In the treatment group, the urethral catheterization needs to be slowly advanced, if the urethral catheterization is blocked, 1-2 mL of the flushing liquid is injected by a syringe, the flushing liquid and the stones are fully contacted for 2 minutes to dissolve, the catheter is pushed forward again, the urethral catheterization can be generally carried out once, if the urethral catheterization is crystalline or sand-shaped, the urethral catheterization can be dissolved, and if the urethral catheterization is small, the urethral catheterization needs to be repeated for 6-7 times, and each time is divided by 2 minutes.

In the control group, after the urinary catheter is inserted into the urethra, a large amount of physiological saline is injected into the urethra, when the pressure reaches a certain level, the urinary catheter is suddenly released and simultaneously presses the abdomen of the cat, so that stones are flushed out, and the operation is repeated for several times until no foreign matters exist in the flushing fluid.

Treatment results: the treatment group had 21 cases, 18 cases were cured, 3 cases were repeatedly developed, and the recurrent cases were followed by the urostomy treatment. The control group had 21 cases, 12 cases were cured, 9 cases were repeatedly developed, and the recurrent cases were followed by the urostomy treatment.

According to the treatment results, the cure rate of the flushing liquid prepared by the method can reach 85.71% after the experiment is carried out, and the cure rate is obviously higher than 57.14% of that of a control group. This shows that the flushing liquid prepared by the application can be used for flushing the animal urinary tract, so that the cure rate of the incomplete blockage of the animal urinary tract can be obviously improved.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.

The acetic acid-acetate acid buffer solution is compounded with components for resisting inflammation, resisting bacteria, easing pain and repairing bladder mucous membrane, and is innovative. Wherein the acetic acid-acetate buffer can dissolve stone, and herba Houttuyniae extract has antiinflammatory and antibacterial effects; the bupleurum root has the functions of relieving fever and pain, resisting inflammation and bacteria and repairing mucous membrane, and the other similar recipe has the components of relieving fever and pain, resisting inflammation and resisting bacteria, and the compound recipe is mixed with acid buffer solution to form the compound recipe with better comprehensive treatment effect.

Furthermore, those skilled in the art will appreciate that while some embodiments herein include some features but not others included in other embodiments, combinations of features of different embodiments are meant to be within the scope of the invention and form different embodiments. For example, in the claims below, any of the claimed embodiments may be used in any combination. The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.

Claims (1)

1. A rinse solution for treating urinary stones in an animal, comprising, per 100mL of the rinse solution: 5mL of cordate houttuynia distillate, 5mL of bupleurum extract, 5g of sodium hyaluronate, 1.09g of acetic acid, 1.16g of sodium acetate and the balance of water for injection;

the preparation method of the cordate houttuynia distillate comprises the following steps: weighing 100g of dried houttuynia cordata, pulverizing into fine powder, adding 500mL of deionized water, placing into a distiller, heating, collecting distilled products, repeating distillation once again, and mixing the distilled products of the two times to obtain houttuynia cordata distillate;

the preparation method of the bupleurum extracting solution comprises the following steps: pulverizing 100g of bupleurum root slices into fine powder, adding 800g of 85% ethanol, reflux-extracting for 3 times at 85 ℃, combining three reflux liquids, and recovering ethanol under reduced pressure until no alcohol smell exists, thus obtaining the bupleurum root extract;

the animal comprises any one of cat and dog;

the urinary calculus comprises magnesium ammonium phosphate calculus;

the preparation method of the flushing liquid for treating animal urinary stones comprises the following steps: heating 85mL of water for injection to 80 ℃, adding 1.09g of acetic acid and 1.16g of sodium acetate, stirring until all the materials are dissolved, sequentially adding 5mL of cordate houttuynia distillate, 5mL of bupleurum root extract and 5g of sodium hyaluronate, and stirring uniformly to obtain a mixed solution; and then the mixed solution is fixed to 100mL, the pH value of the mixed solution is detected to be 4.5, and the flushing fluid for treating animal urinary calculus is obtained through filling, capping and sterilization.

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