CN115005028A - Device and method for collecting pepper rhizosphere secretion - Google Patents
Device and method for collecting pepper rhizosphere secretion Download PDFInfo
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- CN115005028A CN115005028A CN202210730040.6A CN202210730040A CN115005028A CN 115005028 A CN115005028 A CN 115005028A CN 202210730040 A CN202210730040 A CN 202210730040A CN 115005028 A CN115005028 A CN 115005028A
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- 235000002566 Capsicum Nutrition 0.000 title claims abstract description 58
- 239000006002 Pepper Substances 0.000 title claims abstract description 58
- 235000016761 Piper aduncum Nutrition 0.000 title claims abstract description 58
- 235000017804 Piper guineense Nutrition 0.000 title claims abstract description 58
- 235000008184 Piper nigrum Nutrition 0.000 title claims abstract description 58
- 230000028327 secretion Effects 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 15
- 244000203593 Piper nigrum Species 0.000 title 1
- 239000002689 soil Substances 0.000 claims abstract description 61
- 241000722363 Piper Species 0.000 claims abstract description 58
- 230000012010 growth Effects 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 57
- 239000007788 liquid Substances 0.000 claims description 15
- 239000000243 solution Substances 0.000 claims description 14
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- 238000010521 absorption reaction Methods 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 239000012466 permeate Substances 0.000 claims description 4
- 238000013016 damping Methods 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 240000008574 Capsicum frutescens Species 0.000 description 13
- 235000002568 Capsicum frutescens Nutrition 0.000 description 12
- 230000000694 effects Effects 0.000 description 4
- 230000002093 peripheral effect Effects 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241001233242 Lontra Species 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910001410 inorganic ion Inorganic materials 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C23/00—Distributing devices specially adapted for liquid manure or other fertilising liquid, including ammonia, e.g. transport tanks or sprinkling wagons
- A01C23/02—Special arrangements for delivering the liquid directly into the soil
- A01C23/023—Special arrangements for delivering the liquid directly into the soil for liquid or gas fertilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G27/00—Self-acting watering devices, e.g. for flower-pots
- A01G27/04—Self-acting watering devices, e.g. for flower-pots using wicks or the like
- A01G27/06—Self-acting watering devices, e.g. for flower-pots using wicks or the like having a water reservoir, the main part thereof being located wholly around or directly beside the growth substrate
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/12—Supports for plants; Trellis for strawberries or the like
- A01G9/122—Stakes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention discloses a pepper rhizosphere secretion collecting device which comprises a culture box with an opening at the upper end, wherein the upper end of the culture box is of an inverted quadrangular frustum pyramid structure, four limit frames which are arranged in a rectangular manner are arranged in the culture box, each limit frame consists of two vertical rods and a plurality of transverse rods, the transverse rods are fixedly connected between the two corresponding vertical rods, and one side of each vertical rod, which is close to the adjacent inner wall of the culture box, is fixedly connected with a push rod; a method for collecting pepper rhizosphere secretion comprises the following steps: s1, pouring the cultivation soil into the cultivation box, when the cultivation soil is poured to half height, putting the pepper seeds, pushing the collection cylinder upwards to enable the collection bucket to be close to the position of the pepper seeds, and then pouring the cultivation soil. The invention can collect the secretion at different growth stages of the pepper rhizosphere, and ensures that the collected rhizosphere soil contains a large amount of rhizosphere secretion, thereby ensuring the effectiveness of the experiment.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to a device and a method for collecting pepper rhizosphere secretion.
Background
During the growth process of the pepper, the rhizosphere not only takes up nutrients and water from the environment, but also secretes protons into a growth medium, releases inorganic ions, and secretes or secretes a large amount of organic matters. These substances and root tissue casts are collectively referred to as root products, i.e., root secretions. As early as the 18-19 th century, people observed the growth promotion and inhibition of adjacent plants by rhizosphere secretion. However, until the 50 s of the 20 th century, when the mutual relationship between rhizosphere secretion and nitrogen fixation for promoting plant growth was recognized, research in this field became extremely active, and the nature of rhizosphere secretion and the inter-biological relationship between organisms were gradually recognized.
In the research work on pepper rhizosphere secretion, the traditional rhizosphere secretion collecting method is to directly take out the plant rhizosphere from the cultivated soil and then drip-wash the plant rhizosphere with distilled water, and the drip-wash solution is used as the rhizosphere secretion.
Therefore, a device and a method for collecting the pepper rhizosphere secretion are provided to solve the above problems.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a device and a method for collecting pepper rhizosphere secretion.
In order to achieve the purpose, the invention adopts the following technical scheme:
a pepper rhizosphere secretion collecting device comprises a culture box with an opening at the upper end, wherein the upper end of the culture box is of an inverted quadrangular frustum pyramid structure, four limit frames which are arranged in a rectangular manner are arranged in the culture box, each limit frame consists of two vertical rods and a plurality of horizontal rods, the horizontal rods are fixedly connected between the two corresponding vertical rods, one side of each vertical rod, which is close to the adjacent inner wall of the culture box, is fixedly connected with a push rod, the two push rods positioned on the same side penetrate through the inner wall of the corresponding culture box and are fixedly connected with a movable plate together, the peripheral outer walls of the culture box are rotatably connected with threaded rods, one ends of the four threaded rods, which are far away from the culture box, penetrate through the corresponding movable plate and are in threaded connection with the movable plate, a collecting barrel is vertically inserted in the central position of the lower end of the culture box, the collecting barrel is in damping sliding connection with the lower end of the culture box, and the upper end of the collecting barrel is communicated with a collecting hopper, the collecting hopper is located four positions between the limiting frames, the position in the collecting hopper and close to the collecting cylinder is provided with a baffle, the baffle is connected with the inner wall on the right side of the collecting hopper in a rotating mode, the upper end of the baffle and the inner wall on the right side of the collecting hopper are fixedly connected with springs together, a connecting rod is arranged in the collecting cylinder, the upper end of the connecting rod is fixedly connected with an inverted V-shaped soil pulling rod, two sliding openings which are arranged oppositely are formed in the outer peripheral surface of the collecting cylinder and close to the lower end of the collecting cylinder, a pull rod is connected with the sliding openings in a sliding mode, the pull rod is fixedly connected with the connecting rod, and a soil receiving disc is arranged below the collecting cylinder.
Preferably, the fixed frame that has cup jointed in the culture box outside, fixed frame lower extreme fixedly connected with four supporting legs.
Preferably, each threaded rod is fixedly sleeved with a rotating block.
Preferably, four water pipes which are respectively rectangular are arranged in the culture box, each water pipe is attached to the inner wall of the culture box and located between two corresponding push rods, and a water permeable net plate is arranged at one end, close to the limiting frame, of each water pipe.
Preferably, the upper end of the culture box is fixedly connected with a water storage hopper in a shape like a Chinese character 'hui', the upper end of each water pipe is communicated with the lower end of the water storage hopper, and water-absorbing sponges are filled in the water pipes and the positions of the water-permeable net plates.
Preferably, the culture box lower extreme fixedly connected with is located the ring block of collecting vessel outside position, be equipped with the ring form pipe in the ring block, the ring block upper end is equipped with a plurality of rows of water heads with the intercommunication of ring form pipe upper end, ring form pipe lower extreme intercommunication has notes liquid pipe, it runs through the interior diapire setting of culture box and is equipped with annotates the liquid head to annotate liquid pipe lower extreme, it is equipped with the check valve on the liquid pipe to annotate.
A method for collecting pepper rhizosphere secretion comprises the following steps:
s1, pouring the cultivation soil into the cultivation box, when the cultivation soil is poured to half height, putting the pepper seeds, pushing the collection cylinder upwards to enable the collection bucket to be close to the position of the pepper seeds, and then pouring the cultivation soil.
S2, after the pepper seeds are planted, the four threaded rods are sequentially rotated through the four rotating blocks, and the corresponding limiting frames are pushed to move by the aid of the moving plate and the push rods, so that the four limiting frames are arranged in a rectangular mode and surround the pepper seeds.
S3, after the pepper seeds are surrounded by the limiting frame, water can enter each water pipe by adding water into the water storage hopper and be absorbed by the water absorption sponge, so that the water can be conveniently seeped into the cultivation soil from four directions.
S4, injecting the culture solution into the annular tube through the liquid injection tube, so that the culture solution permeates into the cultivating soil from the bottom of the cultivating box, and providing growth nutrients for the pepper rhizosphere.
S5, after the pepper seeds grow into rhizosphere, the collecting cylinder is pulled downwards regularly, the connecting rod is moved upwards by the pull rod in the period, so that the soil pull rod jacks the baffle plate and enters the collecting hopper, then the pull rod is pulled downwards, the soil pull rod is moved downwards, the soil pulling collector cylinder is pushed to the inner top of the collecting hopper and falls onto the soil receiving plate below, the process can be carried out for multiple times, and a certain amount of rhizosphere soil is collected.
S6, separating the secretion in the rhizosphere soil.
Compared with the prior art, the pepper rhizosphere secretion collecting device and the pepper rhizosphere secretion collecting method have the advantages that:
1. can carry on spacingly through four spacing framves to the growth of hot pepper seed, make it can only grow along vertical direction to add the culture solution in to cultivating soil from the culture box bottom, utilize culture solution induction hot pepper rhizosphere to grow, make its growth downwards, then the convenient rhizosphere soil that contains a large amount of hot pepper rhizosphere secretion of effectual collection in rhizosphere below.
2. Through setting up surge drum, pull rod, baffle and collection fill, through the pull rod reciprocating at the sliding opening, usable pull rod jack-up baffle, compression spring to the soil of cultivating that will contain hot pepper rhizosphere secretion draws the surge drum into, and falls to the soil receiving disc of below and collect, and the collection mode is simple and convenient, and can carry out the collection work of secretion in the different stages that hot pepper rhizosphere grows.
In conclusion, the invention can collect the secretion at different growth stages of the pepper rhizosphere, and ensure that the collected rhizosphere soil contains a large amount of rhizosphere secretion, thereby ensuring the effectiveness of the experiment.
Drawings
FIG. 1 is a front perspective view of a device for collecting pepper rhizosphere secretion in accordance with the present invention;
FIG. 2 is an enlarged schematic view of the structure at A in FIG. 1;
FIG. 3 is a perspective view of the front side of a collecting barrel in the device for collecting the secretion of rhizosphere of pepper in accordance with the present invention;
fig. 4 is a schematic structural view of the front side of a limiting frame in the pepper rhizosphere secretion collecting device provided by the invention.
In the figure: the device comprises a culture box 1, a fixed frame 2, a supporting leg 3, a limiting frame 4, a vertical rod 41, a transverse rod 42, a push rod 5, a movable plate 6, a threaded rod 7, a rotating block 8, a water pipe 9, a water permeable screen plate 10, a water absorbing sponge 11, a water storage hopper 12, a collecting barrel 13, a collecting hopper 14, a baffle 15, a spring 16, a connecting rod 17, a soil pulling rod 18, a sliding opening 19, a pull rod 20, a soil receiving disc 21, an annular block 22, an annular pipe 23, a water discharge head 24, a water injection pipe 25 and a one-way valve 26.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-4, a hot pepper rhizosphere secretion collection device, includes that the upper end is open-ended cultivates box 1, cultivates box 1 upper end for the rectangular terrace with edge structure of inversion, makes things convenient for the growth of hot pepper plant, cultivates the fixed frame 2 that has cup jointed in the box 1 outside is fixed, and fixed frame 2 lower extreme fixedly connected with four supporting legs 3 play the effect of supporting cultivateing box 1.
Four limit frames 4 which are arranged in a rectangular shape are arranged in the culture box 1, each limit frame 4 is composed of two vertical rods 41 and a plurality of transverse rods 42, the transverse rods 42 are fixedly connected between the corresponding two vertical rods 41, one side of each vertical rod 41, which is close to the adjacent inner wall of the culture box 1, is fixedly connected with a push rod 5, the two push rods 5 which are positioned at the same side penetrate through the inner wall of the corresponding culture box 1 and are fixedly connected with a movable plate 6 together, threaded rods 7 are rotatably connected on the peripheral outer walls of the culture box 1, one ends of the four threaded rods 7, which are far away from the culture box 1, penetrate through the corresponding movable plate 6 and are in threaded connection with the movable plate, rotating blocks 8 are fixedly sleeved on the threaded rods 7, the threaded rods 7 can be rotated through the rotating blocks 8, so as to drive the movable plate 6 to move, and further push the corresponding limit frames 4 to move under the action of the push rods 5, so that the four limit frames 4 surround pepper seeds, and can be positioned below the limit frames 4 when growing roots, thus growing vertically without shifting.
Cultivate to be equipped with four in the box 1 and be the water pipe 9 that the rectangle is respectively, 1 inner wall setting of box is cultivateed in the equal laminating of each water pipe 9, and be located position between two push rods 5 that correspond, the one end that water pipe 9 is close to spacing 4 is equipped with the otter board 10 that permeates water, cultivate 1 upper end fixedly connected with of box and be the water storage fill 12 of returning the style of calligraphy, each water pipe 9 upper end all communicates with 12 lower extremes of water storage fill, the sponge 11 that absorbs water has all been filled in the position of otter board 10 that just permeates water in each water pipe 9, be used for to adding water in cultivateing box 1, provide the moisture of hot pepper plant growth.
A collecting cylinder 13 is vertically inserted in the center of the lower end of the culture box 1, the collecting cylinder 13 is in damping sliding connection with the lower end of the culture box 1, and the collecting cylinder 13 can move up and down and is kept stable under the condition of not being subjected to large external force. Collecting bucket 14 has been communicated to the upper end of collecting vessel 13, and collecting bucket 14 is located four spacing 4 between the position, and collecting bucket 14 is used for storing the effect that is located hot pepper rhizosphere below and cultivates soil, and the secretion on the hot pepper rhizosphere is most stored in the soil of cultivating of below. The position that just is close to collecting vessel 13 in the collection fill 14 is equipped with baffle 15, and baffle 15 rotates with collecting the inner wall of fighting 14 right side to be connected, and common fixedly connected with spring 16 between baffle 15 upper end and the collecting vessel 14 right side inner wall, baffle 15 play and block the effect that cultivates soil and get into collecting vessel 13, and spring 16 plays the supporting role. Be equipped with connecting rod 17 in the surge drum 13, connecting rod 17 upper end fixedly connected with is the pull soil rod 18 of the type of falling V, on the surge drum 13 outer peripheral face and being close to lower extreme position and offer two relative smooth mouthful 19 that set up about, equal sliding connection has pull rod 20 in each smooth mouthful 19, two pull rods 20 all with connecting rod 17 fixed connection, surge drum 13 below position is equipped with and connects soil tray 21, reciprocate at smooth mouthful 19 through pull rod 20, usable pull soil rod 18 jack-up baffle 15, compression spring 16, and draw in surge drum 13 with the cultivation soil that contains hot pepper rhizosphere secretion, and fall to the soil tray 21 that connects of below, collect.
Cultivate the annular block 22 that 1 lower extreme fixedly connected with of box is located the position of the 13 outside of collecting vessel, be equipped with annular duct 23 in the annular block 22, annular block 22 upper end is equipped with a plurality of drainage head 24 with the intercommunication of annular duct 23 upper end, annular duct 23 lower extreme intercommunication has notes liquid pipe 25, it runs through the setting of cultivateing 1 inner diapire of box and is equipped with and annotates the liquid head to annotate liquid pipe 25 lower extreme, it is equipped with check valve 26 on the liquid pipe 25 to annotate, conveniently to annotate the culture solution into cultivateing box 1, the culture solution has the effect that promotes the growth of hot pepper rhizosphere, and the induced hot pepper rhizosphere grows downwards.
The method for collecting the pepper rhizosphere secretion by adopting the upper collecting device comprises the following steps:
s1, pouring the cultivation soil into the cultivation box 1, when the cultivation soil is poured to half height, putting the pepper seeds, pushing up the collecting cylinder 13 to enable the collecting hopper 14 to be close to the position of the pepper seeds, and then pouring the cultivation soil to enable the pepper seeds to be kept at the center of the cultivation box 1, so that the pepper seeds can grow conveniently.
S2, after the pepper seeds are planted, the four threaded rods 7 are sequentially rotated through the four rotating blocks 8, the corresponding limiting frames 4 are pushed to move by the aid of the moving plate 6 and the push rod 5, the four limiting frames 4 are arranged in a rectangular mode and surround the pepper seeds, the extending of the pepper seeds is limited, and the pepper seeds can only vertically grow, so that collection of secretions is facilitated.
S3, after pepper seeds are surrounded by the limiting frame 4, water can be added into the water storage hopper 12 through the water storage hopper, and the water enters the water pipes 9 and is absorbed by the water absorption sponge 11, so that the water can seep into the cultivation soil from four directions conveniently, water can be continuously supplied into the cultivation soil conveniently, and a good pepper plant production environment can be maintained.
S4, injecting a culture solution into the annular pipe 23 through the liquid injection pipe 25, enabling the culture solution to penetrate into the cultivation soil from the bottom of the cultivation box 1 to provide growth nutrients for the pepper rhizosphere, enabling the culture solution to play a role in promoting the pepper rhizosphere to stretch, inducing the pepper rhizosphere to grow from the lower side, and enabling the pepper rhizosphere to grow downwards.
S5, after the pepper seeds grow into rhizosphere, periodically pulling the collecting cylinder 13 downwards, and during the period, using the pull rod 20 to move the connecting rod 17 upwards, so that the soil pull rod 18 jacks up the baffle 15 and enters the collecting hopper 14, and then pulling the pull rod 20 downwards, so that the soil pull rod 18 moves downwards, so that the cultivated soil in the collecting hopper 14 is pulled to the collecting cylinder 13 and falls onto the soil receiving plate 21 below, and the process can be performed for multiple times, so as to collect a certain amount of rhizosphere soil. The collection of the secretion can be carried out at different stages of the growth of the pepper rhizosphere.
S6, separating the secretion in the rhizosphere soil, wherein the separation mode is the prior art and is not described here.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. The pepper rhizosphere secretion collecting device comprises a culture box (1) with an open upper end, and is characterized in that the upper end of the culture box (1) is of an inverted quadrangular frustum pyramid structure, four limit frames (4) arranged in a rectangular shape are arranged in the culture box (1), each limit frame (4) is composed of two vertical rods (41) and a plurality of transverse rods (42), the transverse rods (42) are fixedly connected between the two corresponding vertical rods (41), one side of each vertical rod (41) close to the adjacent inner wall of the culture box (1) is fixedly connected with a push rod (5), the two push rods (5) positioned on the same side penetrate through the inner wall of the corresponding culture box (1) and are fixedly connected with a movable plate (6), threaded rods (7) are rotatably connected on the outer wall around the culture box (1), and four ends of the threaded rods (7) far away from the culture box (1) penetrate through the corresponding movable plate (6) and are in threaded connection with the movable plate, a collecting barrel (13) is vertically inserted in the center of the lower end of the culture box (1), the collecting barrel (13) is connected with the lower end of the culture box (1) in a damping sliding manner, the upper end of the collecting barrel (13) is communicated with a collecting hopper (14), the collecting hopper (14) is positioned between four limiting frames (4), a baffle (15) is arranged in the collecting hopper (14) and close to the collecting barrel (13), the baffle (15) is rotatably connected with the inner wall of the right side of the collecting hopper (14), a spring (16) is fixedly connected between the upper end of the baffle (15) and the inner wall of the right side of the collecting hopper (14) together, a connecting rod (17) is arranged in the collecting barrel (13), an inverted V-shaped soil pulling rod (18) is fixedly connected to the upper end of the connecting rod (17), two sliding ports (19) which are oppositely arranged left and right are arranged on the outer circumferential surface of the collecting barrel (13) and close to the lower end, and pull rods (20) are connected in each sliding opening (19) in a sliding manner, the two pull rods (20) are fixedly connected with a connecting rod (17), and a soil receiving disc (21) is arranged below the collecting cylinder (13).
2. The pepper rhizosphere secretion collecting device of claim 1, wherein the outer side of the culture box (1) is fixedly sleeved with a fixing frame (2), and the lower end of the fixing frame (2) is fixedly connected with four supporting legs (3).
3. The pepper rhizosphere secretion collecting device of claim 1, wherein each threaded rod (7) is fixedly sleeved with a rotating block (8).
4. The pepper rhizosphere secretion collecting device according to claim 1, wherein four rectangular water pipes (9) are arranged in the culture box (1), each water pipe (9) is attached to the inner wall of the culture box (1) and located between two corresponding push rods (5), and one end, close to the limiting frame (4), of each water pipe (9) is provided with a water permeable screen (10).
5. The pepper rhizosphere secretion collecting device according to claim 4, wherein the upper end of the culture box (1) is fixedly connected with a water storage hopper (12) shaped like a Chinese character 'hui', the upper end of each water pipe (9) is communicated with the lower end of the water storage hopper (12), and a water absorption sponge (11) is filled in the position of the water permeable screen (10) in each water pipe (9).
6. The pepper rhizosphere secretion collecting device of claim 4, wherein the lower end of the culture box (1) is fixedly connected with an annular block (22) which is positioned at the outer side of the collecting cylinder (13), an annular pipe (23) is arranged in the annular block (22), a plurality of drainage heads (24) which are communicated with the upper end of the annular pipe (23) are arranged at the upper end of the annular block (22), a liquid injection pipe (25) is communicated with the lower end of the annular pipe (23), the lower end of the liquid injection pipe (25) penetrates through the inner bottom wall of the culture box (1) and is provided with a liquid injection head, and a one-way valve (26) is arranged on the liquid injection pipe (25).
7. The method for collecting pepper rhizosphere secretion as claimed in any one of the claims 1 to 6, comprising the following steps:
s1, pouring the cultivation soil into the cultivation box (1), when the cultivation soil is poured to half height, putting pepper seeds, pushing up the collecting cylinder (13) to enable the collecting hopper (14) to be close to the position of the pepper seeds, and then pouring the cultivation soil.
S2, after the pepper seeds are planted, the four threaded rods (7) are sequentially rotated through the four rotating blocks (8), and the corresponding limiting frames (4) are pushed to move by the aid of the moving plate (6) and the push rod (5), so that the four limiting frames (4) are arranged in a rectangular mode and surround the pepper seeds.
S3, after the pepper seeds are surrounded by the limiting frame (4), water can be added into the water storage hopper (12) and enters the water pipes (9) and is absorbed by the water absorption sponge (11), so that water can seep into the cultivation soil conveniently from four directions.
S4, injecting the culture solution into the annular pipe (23) through the liquid injection pipe (25), so that the culture solution permeates into the cultivation soil from the bottom of the cultivation box (1) to provide growth nutrients for the pepper rhizosphere.
S5, after the pepper seeds grow into rhizosphere, the collecting cylinder (13) is pulled downwards regularly, the connecting rod (17) is moved upwards by the pull rod (20) in the period, so that the soil pull rod (18) jacks up the baffle (15) to enter the collecting hopper (14), then the pull rod (20) is pulled downwards, the soil pull rod (18) is moved downwards, the soil is jacked up in the collecting hopper (14) to cultivate the soil, the soil pulls the collecting cylinder (13), and the soil falls onto the soil receiving plate (21) below, and the process can be carried out for multiple times, so that a certain amount of rhizosphere soil is collected.
And S6, separating the secretion in the rhizosphere soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210730040.6A CN115005028B (en) | 2022-06-24 | 2022-06-24 | Pepper rhizosphere secretion collecting device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210730040.6A CN115005028B (en) | 2022-06-24 | 2022-06-24 | Pepper rhizosphere secretion collecting device and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115005028A true CN115005028A (en) | 2022-09-06 |
| CN115005028B CN115005028B (en) | 2023-12-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210730040.6A Active CN115005028B (en) | 2022-06-24 | 2022-06-24 | Pepper rhizosphere secretion collecting device and method |
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