CN114964407B - Wild determination device and method for yak metabolic gas - Google Patents

Wild determination device and method for yak metabolic gas Download PDF

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Publication number
CN114964407B
CN114964407B CN202210509807.2A CN202210509807A CN114964407B CN 114964407 B CN114964407 B CN 114964407B CN 202210509807 A CN202210509807 A CN 202210509807A CN 114964407 B CN114964407 B CN 114964407B
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test
gas
yak
collected
wall
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CN114964407A (en
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崔占鸿
刘书杰
孙路
杨得玉
王迅
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Qinghai Academy of Animal Science and Veterinary Medicine
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Qinghai Academy of Animal Science and Veterinary Medicine
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/497Physical analysis of biological material of gaseous biological material, e.g. breath
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01FMEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
    • G01F22/00Methods or apparatus for measuring volume of fluids or fluent solid material, not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K29/00Other apparatus for animal husbandry
    • A01K29/005Monitoring or measuring activity, e.g. detecting heat or mating
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/497Physical analysis of biological material of gaseous biological material, e.g. breath
    • G01N33/4975Physical analysis of biological material of gaseous biological material, e.g. breath other than oxygen, carbon dioxide or alcohol, e.g. organic vapours

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Abstract

The invention discloses a device and a method for field determination of yak metabolic gas, wherein the determination device comprises a test chamber, a test mask is arranged on the outer wall of the test chamber, a breather valve is arranged on the test mask, a test host is arranged on the upper side of the test chamber, the test host is externally connected with a methane analyzer, and the test host and the methane analyzer are externally connected with an air flow controller. According to the invention, the yaks are driven into the test chamber, the activity of the yaks is limited by the test chamber, the arc-shaped plate for storing the feed is arranged to induce the yaks, the head of the yak extends into the test mask, the neck of the yak is clamped by the sealing clamp plate, and the joint of the test mask and the test chamber is sealed by the sealing clamp plate, so that the stability of the yak body is ensured, the leakage of metabolic gas is avoided, the accuracy of a test result is ensured, the oxygen consumption, the discharge amount of carbon dioxide and methane are accurately measured, and the basic data for evaluating the nutritional value and the energy demand of the feed is obtained.

Description

Device and method for field determination of yak metabolic gas
Technical Field
The invention relates to the technical field of ruminant metabolic gas measurement, in particular to a device and a method for field determination of yak metabolic gas.
Background
The respiratory heat measuring device is adopted to research that the heat energy and gas metabolism in the livestock have accumulated to a certain extent, and the research field of ruminant energy substance metabolism has indispensable theoretical basis and guiding significance for the real production in the aspects of evaluating the total nutrient (energy) value of the feed, determining the reasonable energy requirement of the ruminant, establishing the feeding standard, scientifically preparing the feed, improving the feed conversion utilization rate, improving the quality and the production efficiency of livestock products and the like.
The existing breathing metabolism environment-controlled cabin (Chamber) method needs higher infrastructure conditions, and the test animals need to be subjected to strict training adaptation to ensure that the animals can stably measure the metabolic gas, and is not suitable for measuring the metabolic gas of the yaks at different physiological stages under the field test conditions.
Disclosure of Invention
The invention aims to solve the problems in the background art and provides a device and a method for field measurement of yak metabolic gas.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides a yak metabolic gas's open-air survey device, includes the test chamber, be equipped with the test face guard on the outer wall of test chamber, be provided with the breather valve on the test face guard, the upside of test chamber is provided with the test host computer, the test host computer is external to have the methane analyzer, test host computer and methane analyzer are external to have the air current controller, the upside of test face guard is fixed with the filter tank, be equipped with rotatable rotation post in the filter tank, be fixed with two filter element posts on the rotation post, the draught fan that is located the test face guard is installed to the lower extreme of filter tank, the upside of filter tank is connected with the outlet duct, the end connection of outlet duct has an air collection bag, air collection bag passes through the pipe and is connected with the air current controller.
Preferably, the test host adopts an FMS portable animal respiratory metabolism measuring machine.
Preferably, the bottom slides and is provided with the arc in the test face guard, fixedly connected with expanding spring between the inner diapire of arc and test face guard, the lower lateral wall of test face guard slides and inserts and is equipped with the right-angle plate, the tip of right-angle plate and the lower lateral wall fixed connection of arc, the lower lateral wall of test face guard is fixed with the switch that is located right-angle plate tip upside, slide on the outer wall of filter tank and insert and be equipped with the slide, the end fixing of slide has the magnet board, be fixed with the electro-magnet that is located the magnet board outside on the outer wall of test face guard, fixed cover is equipped with the ring gear on the outer wall of rotation post, be equipped with the sawtooth with ring gear engaged with on the outer wall of slide.
Preferably, be fixed with the inlet pipe on the test face guard, the upper end of inlet pipe is fixed with the storage tank, the storage tank internal fixation has the motor, the drive end of motor is fixed with the carousel, the outer wall of carousel rotates and is connected with the connecting rod, the end fixing of connecting rod has the carriage release lever, the tip of carriage release lever slides and inserts and establish in the inlet pipe, the slide slides and inserts and establish on the inlet pipe, and has seted up the opening on the slide.
Preferably, the last lateral wall of filter tank is fixed with the recoil pipe, the intraductal high-pressure draught that lets in of recoil, the tip of recoil pipe and the filter core post sealing contact who is located the right side, the tip and the left filter core post sealing contact of outlet duct, the output and the left filter core post lower extreme sealing contact of draught fan.
Preferably, a motor is fixed on the inner wall of the test chamber, a bidirectional threaded rod is fixed at the driving end of the motor, sliders are connected to two ends of the bidirectional threaded rod in a threaded mode, sealing clamping plates are fixed at the lower ends of the sliders, and arc-shaped openings are formed in the opposite sides of the sealing clamping plates.
Preferably, a guide plate is fixed on the inner wall of the test chamber, the sealing clamping plate is sleeved on the outer wall of the end part of the guide plate in a sliding manner, and a sliding groove is formed in the joint of the sealing clamping plate and the guide plate.
A method for measuring a yak metabolic gas field measuring device comprises the following steps:
s1, connecting all circuits of a test host according to requirements, starting a methane analyzer, an airflow controller and the test host in sequence, and waiting for all displays to be normal;
s2, driving yaks into the test chamber, enabling the heads of the yaks to extend into the test face mask, driving the two-way threaded rods to rotate through the motor to enable the two sealing clamp plates to move towards the inner sides and the necks of the yaks, sealing the contact positions of the test chamber and the yaks, starting to test after the body conditions and the moods of the yaks are stable, guiding out gas exhaled by the yaks into a gas collection bag through an induced draft fan to be stored, feeding the yaks for half an hour every day, collecting the gas for one time every 2 hours, and collecting the gas for 4 times in total, wherein each time lasts for 6min;
s3, measuring and analyzing the methane generation amount, the carbon dioxide emission amount and the oxygen consumption amount:
observing curves of methane quantity, carbon dioxide quantity and oxygen quantity on an interface of a testing host, after the curves are stable, sequentially operating the gas in a plurality of gas collecting bags on a computer, leading the gas out to a methane analyzer and the testing host at a constant speed through a gas flow controller for operation testing, after the gas operation is finished, enabling the curves to operate stably, then performing next gas operation, and stopping program operation and storing gas data for data analysis of subsequent O2, CO2 and CH4 gases until all collected gases finish operation;
the analysis software ExpeData is used for analyzing the animal respiration raw data;
file-Open opens the data file to be analyzed;
2. finding an EXPORTS data output option in the OPEN option, and exporting all original data;
calculating the original data, exporting the original data, pasting the original data into Excel, obtaining the total collected gas quantity of the time according to the time of collecting gas and the processing data recorded in the test process, calculating the collected gas quantity per minute, multiplying the collected gas quantity per minute by 4h for the first wave, multiplying the collected gas quantity per minute by 2h for the second wave, multiplying the collected gas quantity per minute by 2h for the third wave, multiplying the collected gas quantity per minute by 2h for the fourth wave, summing the gas quantities collected in the four stages to 12h total gas collected quantity, and multiplying the gas quantities by 2 to one day total gas collected quantity
Compared with the prior art, the field determination device and method for yak metabolic gas have the advantages that:
according to the invention, the yaks are driven into the test chamber, the activity of the yaks is limited by the test chamber, the arc-shaped plate for storing the feed is arranged to induce the yaks, the head of the yak extends into the test mask, the neck of the yak is clamped by the sealing clamp plate, and the joint of the test mask and the test chamber is sealed by the sealing clamp plate, so that the stability of the yak body is ensured, the leakage of metabolic gas is avoided, the accuracy of a test result is ensured, the oxygen consumption, the discharge amount of carbon dioxide and methane are accurately measured, and the basic data for evaluating the nutritional value and the energy demand of the feed is obtained.
Drawings
Fig. 1 is a schematic structural diagram of a device for field determination of yak metabolic gas provided by the invention;
FIG. 2 is an enlarged view of the structure of portion A of FIG. 1;
FIG. 3 is an enlarged view of the structure of portion B of FIG. 1;
fig. 4 is a top view of a rotating column in the device for measuring the yak metabolic gas in the field;
fig. 5 is an internal side view of a testing chamber in the field testing device for yak metabolic gas provided by the invention.
In the figure: the device comprises a test room 1, a test mask 2, a filter tank 3, a rotating column 4, an induced draft fan 5, a filter core column 6, a recoil pipe 7, an air outlet pipe 8, an air collecting bag 9, an air flow controller 10, a methane analyzer 11, a test host 12, an electromagnet 13, a magnet plate 14, a sliding plate 15, a material storage tank 16, a rotary table 17, a connecting rod 18, a movable rod 19, a feeding pipe 20, an arc plate 21, a right-angle plate 22, a switch 23, a telescopic spring 24, a breather valve 25, a motor 26, a bidirectional threaded rod 27, a slider 28, a sealing clamping plate 29, a guide plate 30 and an arc opening 31.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1-5, a field testing device for yak metabolic gas comprises a testing chamber 1, a testing face mask 2 is arranged on the outer wall of the testing chamber 1, a breather valve 25 is arranged on the testing face mask 2, the breather valve 25 only enters air but does not give out air, the exhaled air is guaranteed to enter a gas collection bag 9 for collection, a motor 26 is fixed on the inner wall of the testing chamber 1, a bidirectional threaded rod 27 is fixed at the driving end of the motor 26, sliding blocks 28 are in threaded connection with the two ends of the bidirectional threaded rod 27, sealing clamping plates 29 are fixed at the lower ends of the sliding blocks 28, arc-shaped openings 31 are formed in the opposite sides of the sealing clamping plates 29, a guide plate 30 is fixed on the inner wall of the testing chamber 1, sealed splint 29 slip cover is established on the tip outer wall of deflector 30, the spout has been seted up with the junction of deflector 30 to sealed splint 29, catch up the ox into to test chamber 1 in, the activity of restriction yak in the test chamber 1, the yak is lured the tempura by the fodder on the arc 21, its head can stretch into to the test face guard 2 in, later starter motor 26 drives two-way threaded rod 27 and rotates, through the screw-thread fit between two-way threaded rod 27 and the slider 28, two sliders 28 all drive sealed splint 29 toward inboard removal and the contact of ox neck, carry out the sealing of test face guard 2, guarantee that the gas of yak exhalation can not take place to leak, the homoenergetic is derived and is collected in the gas collection bag 9.
The upside of test chamber 1 is provided with test host computer 12, test host computer 12 adopts the portable animal of FMS to breathe metabolic measurement machine, test host computer 12 is external to have methane analyzer 11, test host computer 12 and methane analyzer 11 are external to have airflow controller 10, the upside of test face guard 2 is fixed with crosses filter tank 3, it is equipped with rotatable rotation post 4 in the filter tank 3, be fixed with two filter core posts 6 on the rotation post 4, the draught fan 5 that is located test face guard 2 is installed to the lower extreme that crosses filter tank 3, the upside that crosses filter tank 3 is connected with outlet duct 8, the end connection of outlet duct 8 has air collecting bag 9, air collecting bag 9 passes through the pipe and is connected with airflow controller 10.
An arc-shaped plate 21 is arranged at the bottom in the testing face mask 2 in a sliding manner, an expansion spring 24 is fixedly connected between the arc-shaped plate 21 and the inner bottom wall of the testing face mask 2, a right-angle plate 22 is inserted into the lower side wall of the testing face mask 2 in a sliding manner, the end part of the right-angle plate 22 is fixedly connected with the lower side wall of the arc-shaped plate 21, a switch 23 positioned at the upper side of the end part of the right-angle plate 22 is fixed on the lower side wall of the testing face mask 2, a sliding plate 15 is inserted into the outer wall of the filter tank 3 in a sliding manner, a magnet plate 14 is fixed at the end part of the sliding plate 15, an electromagnet 13 positioned at the outer side of the magnet plate 14 is fixed on the outer wall of the testing face mask 2, a toothed ring is fixed on the outer wall of the rotating column 4, sawteeth meshed with the toothed ring are arranged on the outer wall of the sliding plate 15, a back-flushing pipe 7 is fixed on the upper side wall of the filter tank 3, high-pressure airflow is introduced into the back-flushing pipe 7, the end part of the back-flushing pipe 7 is in sealing contact with the filter column 6 positioned at the right side, the end of the air outlet pipe 8 is in sealing contact with the left filter element column 6, the output end of the induced draft fan 5 is in sealing contact with the lower end of the left filter element column 6, the feeding pipe 20 is fixed on the testing face mask 2, the storage tank 16 is fixed at the upper end of the feeding pipe 20, the motor is fixed in the storage tank 16, the rotary disc 17 is fixed at the driving end of the motor, the outer wall of the rotary disc 17 is rotatably connected with the connecting rod 18, the moving rod 19 is fixed at the end of the connecting rod 18, the end of the moving rod 19 is slidably inserted into the feeding pipe 20, the sliding plate 15 is provided with the through hole, the through hole and the feeding pipe 20 are in a staggered state at ordinary times, the feed in the storage tank 16 cannot be led out into the testing face mask 2, the filter element column 6 can filter the lead-out gas, the impurities are prevented from being led out, the feed in the testing face mask 2 is eaten by yaks, the switch 23 is connected with the motor, the electromagnet 13 and an external power supply through a lead wire, when the amount of feed is reduced to a certain value, the right-angle plate 22 is pressed by the reverse elasticity of the telescopic spring 24, at this time, the motor and the electromagnet 13 are powered on, under the action of the magnetic repulsion force between the electromagnet 13 and the magnet plate 14, the sliding plate 15 moves towards the right side, the rotating column 4 is driven to rotate through the meshing of the saw teeth and the toothed ring, the filter element column 6 which is filtered before is transferred to the right side, high-pressure airflow flows downwards to perform recoil work on the filter element column, impurities at the end part of the filter element column 6 are washed out, the filter element column 6 is prevented from being blocked by the impurities, when the sliding plate 15 moves towards the right side, the through hole is communicated with the feed pipe 20, feed in the storage tank 16 flows out to the arc-shaped plate 21 through the feed pipe 20, the motor drives the rotating disc 17 to rotate, the rotating disc 17 drives the moving rod 19 to move up and down in the feed pipe 20 through the connecting rod 18, the feed is prevented from blocking the feed pipe 20, the right-angle plate 22 is extruded under the action of gravity, the action, the switch 23 is pressed, at this time, the motor and the electromagnet 13 is powered on, and the sliding plate 15 moves back.
A method for measuring a yak metabolic gas field measuring device comprises the following steps:
s1, connecting all circuits of a test host 12 as required, starting a methane analyzer 11, an airflow controller 10 and the test host 12 in sequence, and waiting for all displays to be normal;
s2, driving yaks into the testing chamber 1, enabling the heads of the yaks to extend into the testing face mask 2, driving the two-way threaded rod 27 to rotate through the motor 26 to enable the two sealing clamp plates 29 to move inwards and move towards the necks of the yaks, sealing the contact part of the testing chamber 1 and the yaks, starting to perform a test after the body conditions and the moods of the yaks are stable, leading out gas exhaled by the yaks into the gas collection bag 9 through the induced draft fan 5 for storage, collecting the gas after feeding for half an hour every morning, collecting the gas every 2 hours, and collecting the gas for 4 times in total, wherein each time lasts for 6min;
s3, measuring and analyzing the methane generation amount, the carbon dioxide emission amount and the oxygen consumption amount:
observing curves of methane quantity, carbon dioxide quantity and oxygen quantity on an interface of a testing host 12, after the curves are stable, sequentially operating the gas in a plurality of gas collecting bags 9 on a computer, leading the gas out to a methane analyzer 11 and the testing host 12 at a constant speed through a gas flow controller 10 for testing, after the gas operation is finished, enabling the curves to operate stably, and then performing next gas operation until all collected gases finish operation, stopping program operation and storing gas data for subsequent data analysis of O2, CO2 and CH4 gases;
the analysis software ExpeData is used for analyzing the animal respiration raw data;
file-Open opens the data file to be analyzed;
2. finding an EXPORTS data output option in the OPEN option, and exporting all original data;
calculating the original data, then exporting and pasting the original data into Excel, obtaining the total collected gas quantity of the time according to the time of collecting gas and the processing data recorded in the test process, and calculating the collected gas quantity per minute, wherein the first wave of collected gas quantity per minute is multiplied by 4h, the second wave of collected gas quantity per minute is multiplied by 2h, the third wave of collected gas quantity per minute is multiplied by 2h, the fourth wave of collected gas quantity per minute is multiplied by 4h, the gas quantities collected in the four stages are summed up to 12h of total gas collected quantity, and then multiplied by 2 to obtain the total gas collected quantity in one day.
Further, unless otherwise specifically stated or limited, the above-described fixed connection is to be understood in a broad sense, and may be, for example, welded, glued, or integrally formed as is conventional in the art.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (6)

1. The utility model provides a yak metabolic gas's open-air survey device, includes test chamber (1), its characterized in that, be equipped with test mask (2) on the outer wall of test chamber (1), be provided with breather valve (25) on test mask (2), the upside of test chamber (1) is provided with test host (12), test host (12) are external to have methane analyzer (11), test host (12) and methane analyzer (11) are external to have air current controller (10), the upside of test mask (2) is fixed with filter tank (3), be equipped with rotatable rotation post (4) in filter tank (3), be fixed with two filter core posts (6) on rotation post (4), draught fan (5) that are located test mask (2) are installed to the lower extreme of filter tank (3), the upside of filter tank (3) is connected with outlet duct (8), the end connection of outlet duct (8) has air collection bag (9), air collection bag (9) pass through the pipe and are connected with air current controller (10);
an arc-shaped plate (21) is arranged at the bottom in the test face mask (2) in a sliding mode, a telescopic spring (24) is fixedly connected between the arc-shaped plate (21) and the inner bottom wall of the test face mask (2), a right-angle plate (22) is inserted into the lower side wall of the test face mask (2) in a sliding mode, the end portion of the right-angle plate (22) is fixedly connected with the lower side wall of the arc-shaped plate (21), a switch (23) located on the upper side of the end portion of the right-angle plate (22) is fixed to the lower side wall of the test face mask (2), a sliding plate (15) is inserted into the outer wall of the filter tank (3) in a sliding mode, a magnet plate (14) is fixed to the end portion of the sliding plate (15), an electromagnet (13) located on the outer side of the magnet plate (14) is fixed to the outer wall of the test face mask (2), a toothed ring is arranged on the outer wall of the rotating column (4), and sawteeth meshed with the toothed ring are arranged on the outer wall of the sliding plate (15);
be fixed with inlet pipe (20) on test face guard (2), the upper end of inlet pipe (20) is fixed with storage tank (16), storage tank (16) internal fixation has the motor, the drive end of motor is fixed with carousel (17), the outer wall rotation of carousel (17) is connected with connecting rod (18), the end fixing of connecting rod (18) has carriage release lever (19), the tip of carriage release lever (19) slides and inserts and establish in inlet pipe (20), slide (15) slide and insert and establish on inlet pipe (20), and seted up the opening on slide (15).
2. The yak metabolic gas field determination device according to claim 1, wherein the test host (12) adopts an FMS portable animal respiratory metabolism measuring machine.
3. The yak metabolic gas field testing device of claim 1, characterized in that, the last lateral wall of filter tank (3) is fixed with recoil pipe (7), let in high pressure draught in recoil pipe (7), the tip of recoil pipe (7) and the filter stem post (6) sealing contact that are located the right side, the tip and the left filter stem post (6) sealing contact of outlet duct (8), the output and the left filter stem post (6) lower extreme sealing contact of draught fan (5).
4. The yak metabolic gas field determination device according to claim 1, wherein a motor (26) is fixed on the inner wall of the test chamber (1), a bidirectional threaded rod (27) is fixed at the driving end of the motor (26), two ends of the bidirectional threaded rod (27) are both in threaded connection with a sliding block (28), a sealing clamping plate (29) is fixed at the lower end of the sliding block (28), and an arc-shaped opening (31) is formed in the opposite side of the sealing clamping plate (29).
5. The yak metabolic gas field testing device according to claim 4, wherein a guide plate (30) is fixed on the inner wall of the testing chamber (1), the sealing clamping plate (29) is slidably sleeved on the outer wall of the end portion of the guide plate (30), and a sliding groove is formed in the connecting portion of the sealing clamping plate (29) and the guide plate (30).
6. The method for testing the yak metabolic gas field testing device according to any one of claims 1-5, which is characterized by comprising the following steps:
s1, connecting all circuits of a test host (12) as required, starting a methane analyzer (11), an airflow controller (10) and the test host (12) in sequence, and waiting for all displays to be normal;
s2, driving the yak into the test chamber (1), extending the head of the yak into the test mask (2), driving the two-way threaded rod (27) to rotate through the motor (26) to enable the two sealing clamp plates (29) to move towards the inner side and to be connected with the cow neck, sealing the contact position of the test chamber (1) and the yak,
after the body condition and emotion of the yak are stable, the test is started, the gas exhaled by the yak is guided out into a gas collection bag (9) through an induced draft fan (5) to be stored, the yak is fed for half an hour every morning and then is collected, the gas is collected every 2 hours, and the yak is collected for 4 times every 6 minutes;
and S3, measuring and analyzing the methane generation amount, the carbon dioxide emission amount and the oxygen consumption amount: observing curves of methane quantity, carbon dioxide quantity and oxygen quantity of an interface of a testing host (12), after the curves are stabilized, sequentially operating the gas in a plurality of gas collecting bags (9), leading the gas out to a methane analyzer (11) and the testing host (12) at a constant speed through a gas flow controller (10) for operation testing, after the gas operation is finished, enabling the curves to operate stably, and then performing next gas operation until all collected gases operate completely, stopping program operation and storing gas data for subsequent O2, CO2 and CH4 gas data analysis;
the analysis software ExpeData is used for analyzing the animal respiration raw data;
file-Open opens the data file to be analyzed;
2. searching an EXPORTS data output option in the OPEN option, and exporting all original data; calculating the original data, then leading out and pasting the original data into Excel, obtaining the total collected gas quantity of the time according to the time of collecting gas and the processing data recorded in the test process, and calculating the collected gas quantity per minute, wherein the collected gas quantity per minute of the first wave is multiplied by 4h, the collected gas quantity per minute of the second wave is multiplied by 2h, the collected gas quantity per minute of the third wave is multiplied by 2h, the collected gas quantity per minute of the fourth wave is multiplied by 4h, the gas quantities collected in the four stages are added up to be the total gas collected quantity of 12h, and then multiplied by 2 to be the total gas collected quantity of one day.
CN202210509807.2A 2022-05-11 2022-05-11 Wild determination device and method for yak metabolic gas Expired - Fee Related CN114964407B (en)

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Application Number Priority Date Filing Date Title
CN202210509807.2A CN114964407B (en) 2022-05-11 2022-05-11 Wild determination device and method for yak metabolic gas
NL2034810A NL2034810A (en) 2022-05-11 2023-05-11 Apparatus and method for measuring metabolic gases in yaks in the field

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Application Number Priority Date Filing Date Title
CN202210509807.2A CN114964407B (en) 2022-05-11 2022-05-11 Wild determination device and method for yak metabolic gas

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CN114964407A CN114964407A (en) 2022-08-30
CN114964407B true CN114964407B (en) 2022-11-29

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JPH06347305A (en) * 1993-06-08 1994-12-22 Hitachi Ltd Method and device for extracting minute quantity
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