CN114957349A - Pretreatment and filtration method of acarbose fermentation liquor - Google Patents
Pretreatment and filtration method of acarbose fermentation liquor Download PDFInfo
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- XUFXOAAUWZOOIT-SXARVLRPSA-N (2R,3R,4R,5S,6R)-5-[[(2R,3R,4R,5S,6R)-5-[[(2R,3R,4S,5S,6R)-3,4-dihydroxy-6-methyl-5-[[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)-1-cyclohex-2-enyl]amino]-2-oxanyl]oxy]-3,4-dihydroxy-6-(hydroxymethyl)-2-oxanyl]oxy]-6-(hydroxymethyl)oxane-2,3,4-triol Chemical compound O([C@H]1O[C@H](CO)[C@H]([C@@H]([C@H]1O)O)O[C@H]1O[C@@H]([C@H]([C@H](O)[C@H]1O)N[C@@H]1[C@@H]([C@@H](O)[C@H](O)C(CO)=C1)O)C)[C@@H]1[C@@H](CO)O[C@@H](O)[C@H](O)[C@H]1O XUFXOAAUWZOOIT-SXARVLRPSA-N 0.000 title claims abstract description 51
- 229960002632 acarbose Drugs 0.000 title claims abstract description 51
- XUFXOAAUWZOOIT-UHFFFAOYSA-N acarviostatin I01 Natural products OC1C(O)C(NC2C(C(O)C(O)C(CO)=C2)O)C(C)OC1OC(C(C1O)O)C(CO)OC1OC1C(CO)OC(O)C(O)C1O XUFXOAAUWZOOIT-UHFFFAOYSA-N 0.000 title claims abstract description 51
- 238000000855 fermentation Methods 0.000 title claims abstract description 48
- 230000004151 fermentation Effects 0.000 title claims abstract description 48
- 238000001914 filtration Methods 0.000 title claims abstract description 28
- 238000002203 pretreatment Methods 0.000 title claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 31
- 239000000706 filtrate Substances 0.000 claims abstract description 21
- 238000003825 pressing Methods 0.000 claims abstract description 13
- 238000003756 stirring Methods 0.000 claims description 20
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 18
- 238000005406 washing Methods 0.000 claims description 13
- 239000000276 potassium ferrocyanide Substances 0.000 claims description 7
- XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 claims description 7
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 7
- 229960001763 zinc sulfate Drugs 0.000 claims description 7
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 150000007522 mineralic acids Chemical class 0.000 claims description 6
- 150000007524 organic acids Chemical class 0.000 claims description 6
- 235000006408 oxalic acid Nutrition 0.000 claims description 6
- 239000003651 drinking water Substances 0.000 claims description 5
- 235000020188 drinking water Nutrition 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 239000000264 sodium ferrocyanide Substances 0.000 claims description 4
- GTSHREYGKSITGK-UHFFFAOYSA-N sodium ferrocyanide Chemical compound [Na+].[Na+].[Na+].[Na+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] GTSHREYGKSITGK-UHFFFAOYSA-N 0.000 claims description 4
- 235000012247 sodium ferrocyanide Nutrition 0.000 claims description 4
- 239000010451 perlite Substances 0.000 claims description 3
- 235000019362 perlite Nutrition 0.000 claims description 3
- 229920002401 polyacrylamide Polymers 0.000 claims description 3
- 239000008394 flocculating agent Substances 0.000 claims description 2
- 238000001471 micro-filtration Methods 0.000 abstract description 11
- 239000000919 ceramic Substances 0.000 abstract description 10
- 239000012528 membrane Substances 0.000 abstract description 10
- 239000007788 liquid Substances 0.000 abstract description 9
- 238000005265 energy consumption Methods 0.000 abstract description 6
- 102000004169 proteins and genes Human genes 0.000 abstract description 4
- 108090000623 proteins and genes Proteins 0.000 abstract description 4
- 239000012535 impurity Substances 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 6
- 229920001542 oligosaccharide Polymers 0.000 description 4
- 150000002482 oligosaccharides Chemical class 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 108010028144 alpha-Glucosidases Proteins 0.000 description 3
- 239000012065 filter cake Substances 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 102000016679 alpha-Glucosidases Human genes 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- DBTMGCOVALSLOR-UHFFFAOYSA-N 32-alpha-galactosyl-3-alpha-galactosyl-galactose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(OC2C(C(CO)OC(O)C2O)O)OC(CO)C1O DBTMGCOVALSLOR-UHFFFAOYSA-N 0.000 description 1
- 229940077274 Alpha glucosidase inhibitor Drugs 0.000 description 1
- RXVWSYJTUUKTEA-UHFFFAOYSA-N D-maltotriose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 RXVWSYJTUUKTEA-UHFFFAOYSA-N 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 102100022624 Glucoamylase Human genes 0.000 description 1
- 102400000471 Isomaltase Human genes 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- 102100024295 Maltase-glucoamylase Human genes 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 108010026867 Oligo-1,6-Glucosidase Proteins 0.000 description 1
- 101710184309 Probable sucrose-6-phosphate hydrolase Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 102400000472 Sucrase Human genes 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 101710112652 Sucrose-6-phosphate hydrolase Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 239000003888 alpha glucosidase inhibitor Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 1
- 210000000110 microvilli Anatomy 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/203—Monocyclic carbocyclic rings other than cyclohexane rings; Bicyclic carbocyclic ring systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
Abstract
The invention provides a pretreatment and filtration method of acarbose fermentation liquor, through a proper pretreatment method, impurities affecting filtration such as water-soluble protein in the acarbose fermentation liquor are firstly precipitated, then other proteins and hyphae are flocculated, filtrate is obtained through a plate-and-frame filter pressing mode, as the plate-and-frame filter pressing process basically does not generate heat, feed liquid does not need to be cooled in the whole process, and the pump power used for plate-and-frame filter pressing is much smaller than that used for ceramic membrane microfiltration, the comprehensive energy consumption can be greatly reduced and is reduced by more than 70% compared with the ceramic membrane microfiltration method; meanwhile, the method can solve the problem of collecting fermentation filter residues while obtaining acarbose fermentation filtrate; the method is simple and easy to operate, has low requirement on equipment, is environment-friendly and is convenient for industrial production.
Description
Technical Field
The invention relates to the technical field of bioengineering, in particular to a pretreatment and filtration method of acarbose fermentation liquor.
Background
Acarbose is an alpha-glucosidase inhibitor, a complex oligosaccharide.
Acarbose, an important API, is mainly used for lowering blood sugar, and it competes with oligosaccharides at the brush border of upper cells of the small intestine to reversibly bind to α -glucosidase, inhibiting the activity of various α -glucosidases such as maltase, isomaltase, glucoamylase and sucrase, and decomposing starch into oligosaccharides such as maltose (disaccharide), maltotriose and dextrin (oligosaccharide) and then into glucose, and reducing the decomposition rate of sucrose into glucose and fructose, thereby causing the absorption of glucose in the intestinal tract to be slowed down, thereby relieving postprandial hyperglycemia and achieving the effect of lowering blood sugar.
Acarbose is a product produced by a fermentation method, because acarbose fermentation liquor is viscous, hyphae are very fine, and the filter pressing is not easy to be carried out by traditional methods such as plate and frame methods, the current common pretreatment and filtration method is a microfiltration method, the fermentation liquor is subjected to microfiltration by a ceramic membrane to obtain filtrate, and the filtrate is separated and purified to obtain the high-purity acarbose.
Because the power of a pump motor used by the ceramic membrane is very high, a large amount of heat is generated in the microfiltration process, acarbose is unstable to heat and needs to be cooled in the whole process, and therefore the energy consumption of the whole microfiltration process is very high. The filter residue obtained by ceramic membrane treatment is thick slurry liquid, the solid content is about 50 percent (wet solid content), and dry filter residue cannot be obtained, so the filter residue needs to be further subjected to filter pressing treatment to obtain dry filter residue for further treatment.
Therefore, a pretreatment and filtration method of acarbose fermentation liquor is needed, which can solve the problems of high energy consumption and poor filtration effect of the existing ceramic membrane microfiltration method for treating the acarbose fermentation liquor.
Disclosure of Invention
The invention aims to provide a pretreatment and filtration method of acarbose fermentation liquor, and aims to solve the problems of high energy consumption and poor filtration effect of the existing ceramic membrane microfiltration method for treating the acarbose fermentation liquor.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a pretreatment and filtration method of acarbose fermentation liquor, which comprises the following steps:
s1, cooling acarbose fermentation liquor to 5-20 ℃;
s2, adjusting the pH value of the acarbose fermentation liquor obtained in the step S1 to 2-5 by using organic acid or inorganic acid;
s3, adding 0.1-1 wt% of potassium ferrocyanide or sodium ferrocyanide into the acarbose fermentation liquor obtained in the step S2, and stirring for dissolving;
s4, adding 0.1-1 wt% of zinc sulfate into the acarbose fermentation liquor obtained in the step S3, and stirring for dissolving;
s5, adding 0.5-5 wt% of diatomite or perlite into the acarbose fermentation liquor obtained in the step S4, and uniformly stirring;
s6, adding 0.01-0.1 wt% of flocculating agent into the acarbose fermentation liquor obtained in the step S5, and uniformly stirring;
s7, carrying out filter pressing on the acarbose fermentation liquor obtained in the step S6 by using a plate frame to obtain a filtrate;
and S8, carrying out top washing on the plate frame by using drinking water to obtain top washing filtrate.
Preferably, in step S1, cooling is performed to 10-15 ℃.
Preferably, in step S2, oxalic acid, citric acid, sulfuric acid, hydrochloric acid are used as the organic acid or inorganic acid.
More preferably, in step S2, oxalic acid is used as the organic acid or the inorganic acid.
Preferably, in step S2, the pH is adjusted to 3-4.
Preferably, in step S3, 0.2 to 0.3% by weight of potassium ferrocyanide or sodium ferrocyanide is added.
Preferably, in step S4, 0.1-0.3 wt% of zinc sulfate is added.
Preferably, in step S6, polyaluminium chloride or polyacrylamide is used as the flocculant.
More preferably, in step S6, the flocculant is polyaluminum chloride.
Preferably, in step S6, 0.02-0.04 wt% of flocculant is added.
Compared with the prior art, the invention has the following beneficial technical effects:
according to the pretreatment and filtration method of the acarbose fermentation liquor, impurities affecting filtration such as water-soluble protein in the acarbose fermentation liquor are firstly precipitated through a proper pretreatment method, other proteins and hyphae are flocculated, filtrate is obtained through a plate-and-frame filter pressing mode, heat is basically not generated in the plate-and-frame filter pressing process, feed liquid does not need to be cooled in the whole process, and the pump power used for plate-and-frame filter pressing is much smaller than that used for ceramic membrane microfiltration, so that the comprehensive energy consumption is greatly reduced and is reduced by more than 70% compared with that of a ceramic membrane microfiltration method; meanwhile, the method can solve the problem of collecting fermentation filter residues while obtaining acarbose fermentation filtrate; the method is simple and easy to operate, has low requirement on equipment, is environment-friendly and is convenient for industrial production.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention aims to provide a pretreatment and filtration method of acarbose fermentation liquor, which can solve the problems of high energy consumption and poor filtration effect of the existing ceramic membrane microfiltration method for treating the acarbose fermentation liquor.
The present invention will be described in further detail with reference to specific embodiments in order to make the above objects, features and advantages more apparent and understandable.
Example 1:
taking 50L acarbose fermentation liquor, cooling to 6 deg.C, adjusting pH to 3.05 with oxalic acid, adding 100g potassium ferrocyanide, stirring for 30min, adding 100g zinc sulfate, stirring for 30min, adding 1000g diatomaceous earth, stirring for 15min, adding 20g polyaluminium chloride, and stirring for 30 min. And (3) carrying out filter pressing on the pretreatment solution through a diaphragm type plate-and-frame filter press to obtain 38L of filtrate, then carrying out top washing on the filter cake through drinking water to obtain 30L of top washing liquid, combining the top washing liquid with the filtrate to obtain 68L of filtrate and 14kg of filter residue, detecting the content of the acarbose in the filtrate through a liquid phase, and obtaining the filtration yield of 96.2%.
Example 2:
taking 100L acarbose fermentation liquor, cooling to 5 ℃, adjusting pH to 3.12 with oxalic acid, adding 180g potassium ferrocyanide, stirring for 30min, adding 180g zinc sulfate, stirring for 30min, adding 2000g diatomite, stirring for 15min, adding 40g polyaluminium chloride, and stirring for 30 min. And (3) carrying out filter pressing on the pretreatment solution through a diaphragm type plate-and-frame filter press to obtain 79L of filtrate, then carrying out top washing on the filter cake through drinking water to obtain 60L of top washing liquid, combining the top washing liquid with the filtrate to obtain 139L of filtrate and 29kg of filter residue, and detecting the content of the acarbose in the filtrate through a liquid phase, wherein the filtration yield is 95.3%.
Example 3:
taking 100L acarbose fermentation liquor, cooling to 5 deg.C, adjusting pH to 3.11 with hydrochloric acid, adding 180g potassium ferrocyanide, stirring for 30min, adding 180g zinc sulfate, stirring for 30min, adding 2000g perlite, stirring for 15min, adding 40g polyacrylamide, and stirring for 30 min. And (3) carrying out filter pressing on the pretreatment solution through a diaphragm type plate-and-frame filter press to obtain 72L of filtrate, then carrying out top washing on the filter cake with drinking water to obtain 70L of top washing liquid, combining the top washing liquid with the filtrate to obtain 142L of filtrate and 32kg of filter residue, wherein the filtration yield is 91.7%.
The principle and the implementation mode of the invention are explained by applying specific examples, and the description of the above examples is only used for helping understanding the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In summary, this summary should not be construed to limit the present invention.
Claims (10)
1. A pretreatment and filtration method of acarbose fermentation liquor is characterized in that: the method comprises the following steps:
s1, cooling acarbose fermentation liquor to 5-20 ℃;
s2, adjusting the pH value of the acarbose fermentation liquor obtained in the step S1 to 2-5 by using organic acid or inorganic acid;
s3, adding 0.1-1 wt% of potassium ferrocyanide or sodium ferrocyanide into the acarbose fermentation liquor obtained in the step S2, and stirring for dissolving;
s4, adding 0.1-1 wt% of zinc sulfate into the acarbose fermentation liquor obtained in the step S3, and stirring for dissolving;
s5, adding 0.5-5 wt% of diatomite or perlite into the acarbose fermentation liquor obtained in the step S4, and uniformly stirring;
s6, adding 0.01-0.1 wt% of flocculating agent into the acarbose fermentation liquor obtained in the step S5, and uniformly stirring;
s7, carrying out filter pressing on the acarbose fermentation liquor obtained in the step S6 by using a plate frame to obtain a filtrate;
and S8, carrying out top washing on the plate frame by using drinking water to obtain top washing filtrate.
2. The method for pretreating and filtering an acarbose fermentation broth according to claim 1, wherein: in step S1, the temperature is cooled to 10-15 ℃.
3. The method for the pretreatment and filtration of an acarbose fermentation broth according to claim 1, wherein: in step S2, oxalic acid, citric acid, sulfuric acid, or hydrochloric acid is used as the organic acid or the inorganic acid.
4. The method for pretreating and filtering an acarbose fermentation broth according to claim 3, wherein: in step S2, oxalic acid is used as the organic acid or the inorganic acid.
5. The method for pretreating and filtering an acarbose fermentation broth according to claim 1, wherein: in step S2, the pH is adjusted to 3-4.
6. The method for pretreating and filtering an acarbose fermentation broth according to claim 1, wherein: in step S3, 0.2-0.3 wt% of potassium ferrocyanide or sodium ferrocyanide is added.
7. The method for pretreating and filtering an acarbose fermentation broth according to claim 1, wherein: in step S4, 0.1-0.3 wt% of zinc sulfate is added.
8. The method for pretreating and filtering an acarbose fermentation broth according to claim 1, wherein: in step S6, polyaluminium chloride and polyacrylamide are used as the flocculant.
9. The process for the pretreatment and filtration of acarbose fermentation broth according to claim 8, wherein: in step S6, polyaluminum chloride is used as the flocculant.
10. The method for pretreating and filtering an acarbose fermentation broth according to claim 1, wherein: in step S6, 0.02-0.04 wt% of a flocculant is added.
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2022
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