CN114950581B - Multi-track spiral microfluidic chip and manufacturing method thereof - Google Patents
Multi-track spiral microfluidic chip and manufacturing method thereof Download PDFInfo
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- CN114950581B CN114950581B CN202210353905.1A CN202210353905A CN114950581B CN 114950581 B CN114950581 B CN 114950581B CN 202210353905 A CN202210353905 A CN 202210353905A CN 114950581 B CN114950581 B CN 114950581B
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 19
- 238000003698 laser cutting Methods 0.000 claims abstract description 4
- 238000004806 packaging method and process Methods 0.000 claims abstract description 4
- 238000005516 engineering process Methods 0.000 claims description 7
- 238000005192 partition Methods 0.000 claims description 5
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 4
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims description 4
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 4
- 238000005452 bending Methods 0.000 claims description 3
- 238000005530 etching Methods 0.000 claims description 3
- 238000005459 micromachining Methods 0.000 claims description 3
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims description 3
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims description 3
- 238000002174 soft lithography Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 9
- 238000001514 detection method Methods 0.000 abstract description 4
- 238000009434 installation Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 description 7
- 238000000465 moulding Methods 0.000 description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000007731 hot pressing Methods 0.000 description 2
- 238000001259 photo etching Methods 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005323 electroforming Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229920002120 photoresistant polymer Polymers 0.000 description 1
- -1 polydimethylsiloxane Polymers 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micromachines (AREA)
Abstract
The invention discloses a multi-track spiral micro-fluidic chip and a manufacturing method thereof, comprising the following steps: cover plate, interior track panel and bottom plate. The inner track panel is provided with a complete straight micro-channel, a channel inlet and two channel outlets, and the straight track is made into a spiral track through rotation after installation; the bottom plate and other plates are provided with positioning holes. The manufacturing method comprises the following steps: laser cutting and forming of the plate; microfluidic chip assembly and packaging methods. The microfluidic chip provided by the invention has the advantages of novel structure, small occupied space and low manufacturing cost, and is suitable for large-flow commercial detection.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a multi-track spiral microfluidic chip and a manufacturing method thereof.
Background
The microfluidic chip can be replicated in a large scale by a molding method and a hot-pressing method, so that the manufacturing efficiency is greatly improved, and the manufacturing cost is reduced, but a silicon mold or a metal mold used by the molding method or the hot-pressing method is manufactured by adopting a silicon process and a photoetching electroforming injection molding process, and the mold manufacturing process is complex and has high cost. The micro-fluidic chip is manufactured by taking the photoresist structure as a mould and polydimethylsiloxane as a chip material through a molding method, and has low cost and short manufacturing period.
At present, the existing microfluidic chip has low sorting and enriching speed due to the self structure, and cannot meet the large-scale use requirement, and the increase of the speed by simply stacking layers can cause the occupied volume of the chip to be too large, so that the efficiency and portability cannot be achieved. Meanwhile, the flow velocity of each flow channel cannot be controlled by improving the enrichment and separation efficiency through overlapping the layers, so that the enrichment and separation accuracy is reduced.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a multi-track spiral micro-fluidic chip and a manufacturing method thereof, which solve the problem that the sorting and enrichment efficiency and the portability of the chip cannot be achieved, improve the efficiency and ensure the accuracy.
In order to solve the technical problems, the invention provides the following technical scheme:
the utility model provides a multichannel spiral micro-fluidic chip which characterized in that: including apron, interior track panel and the bottom plate that stacks in proper order and set up, apron, interior track panel and bottom plate are as a whole to be bent into helical structure, are provided with runner entry and runner export at the both ends of interior track panel, are provided with a plurality of micro-channels at the middle part of interior track panel, pass through between runner entry and the runner export micro-channel intercommunication each other.
Further, the number of the micro flow channels is n, n is greater than 3, the micro flow channels are arranged on the inner track panel in parallel, and the flow channel inlets are communicated with the micro flow channels through the tree-shaped bifurcation flow channels.
Further, a partition plate is arranged at the runner outlet, the partition plate divides the runner outlet into a first runner outlet and a second runner outlet, and the first runner outlet and the second runner outlet are respectively positioned at two sides of the inner runner panel.
Further, the cover plate, the inner track panel and the bottom plate are sequentially arranged from outside to inside on the spiral structure.
Further, the cover plate, the inner track panel and the bottom plate are respectively provided with a first positioning hole, a second positioning hole and a third positioning hole, and the first positioning holes, the second positioning holes and the third positioning holes are mutually connected.
The manufacturing method of the multi-track spiral microfluidic chip comprises the following steps:
step 1: etching and exposing by a laser micro-machining technology ultraviolet laser cutting or a PDMS soft lithography technology, manufacturing a cover plate, an inner track surface plate and a bottom plate, and placing the bottom plate in a tool;
step 2: bonding the inner track panel to the base plate;
step 3: the cover plate is bonded with the inner channel panel to complete chip packaging, and a complete channel inlet, n straight micro channels, a first channel outlet and a second channel outlet are formed;
step 4: and (3) rotating and bending the whole chip to form the final multi-track spiral microfluidic core.
Compared with the prior art, the invention has the beneficial effects that: 1. compared with the superposition of multiple chips, the multi-track spiral microfluidic chip provided by the invention has the advantages that the distance between tracks is greatly reduced, so that the whole chip volume is reduced, the simultaneous separation and enrichment of multiple tracks can be realized, and the detection efficiency is greatly improved. 2. The flow distribution technology applied in the invention solves the problem of non-uniform flow velocity among the flow channels, the flow velocity of each flow channel can be controlled to be consistent through the tree-shaped branched flow channels, and the flow velocity of the micro flow channel is directly controlled through controlling the flow velocity of the inlet of the flow channel, so that the enrichment and separation accuracy is improved, the enrichment and separation operation is easier to control, and the detection accuracy is improved compared with that of a stacked chip. 3. The linear chip is manufactured firstly, then the spiral is bent after being laminated, and compared with the method for directly manufacturing the spiral chip, the method has the advantages that the photoetching difficulty is greatly reduced, and the manufacturing period is shortened. 4. The number of flow channels n (n > 3) of the chip can be determined according to the requirements, so that the chip can be manufactured more flexibly, and more possible requirements can be met.
Drawings
FIG. 1 is a schematic diagram of a three-dimensional structure of a multi-track spiral microfluidic chip according to the present invention;
FIG. 2 is a top view of a multi-track spiral microfluidic chip of the present invention;
FIG. 3 is a schematic view of a planar unfolding structure of the multi-track spiral microfluidic chip before being bent into a spiral shape;
FIG. 4 is a schematic diagram of the explosive structure of FIG. 3;
fig. 5 is a schematic view of the inner track panel of fig. 4 on the side near the bottom plate.
Fig. 6 is a schematic view of the inner track panel of fig. 4 on the side of the cover.
Wherein: the device comprises a cover plate 1, an inner track panel 2, a bottom plate 3, a runner inlet 4, a micro runner 5, a tree-shaped branched runner 6, a partition plate 7, a first runner outlet 8, a second runner outlet 9, a first positioning hole 10, a second positioning hole 11 and a third positioning hole 12.
Detailed Description
In order to enhance the understanding of the present invention, the present invention will be further described in detail with reference to the drawings, which are provided for the purpose of illustrating the present invention only and are not to be construed as limiting the scope of the present invention.
Fig. 1-6 show a specific embodiment of a multi-track spiral microfluidic chip, which comprises a cover plate 1, an inner track panel 2 and a bottom plate 3 which are sequentially stacked, wherein the cover plate 1, the inner track panel 2 and the bottom plate 3 are folded into a spiral structure as a whole, a runner inlet 4 and a runner outlet are arranged at two ends of the inner track panel 2, a plurality of micro runners 5 are arranged in the middle of the inner track panel 2, and the runner inlet 4 and the runner outlet are mutually communicated through the micro runners 5.
Preferably, the number of the micro-channels 5 is 4, each micro-channel 5 is arranged on the inner track panel 2 in parallel, the channel inlets 4 and the micro-channels 5 are communicated through the tree-shaped branching channels 6, and 1 channel inlet 4 is branched into 4 parallel micro-channels 5 through the tree-shaped branching channels 6. The flow velocity of each micro-channel 5 is consistent, the flow velocity of the micro-channel 5 is directly controlled by controlling the flow velocity of the inlet 4 of the flow channel, so that the enrichment and separation accuracy is improved, the enrichment and separation operation is easier to control, and the detection accuracy is improved compared with that of a stacked chip. The flow channel outlet is provided with a baffle 7, the baffle 7 divides the flow channel outlet into a first flow channel outlet 8 and a second flow channel outlet 9, the first flow channel outlet 8 and the second flow channel outlet 9 are respectively positioned on two sides of the inner track panel 2, the first flow channel outlet 8 is positioned on one side of the cover plate 1, and the second flow channel outlet 9 is positioned on one side of the bottom plate 3. The cover plate 1, the inner track panel 2 and the bottom plate 3 are sequentially arranged from outside to inside on a spiral structure. The cover plate 1, the inner rail surface plate 2 and the bottom plate 3 are respectively provided with a first positioning hole 10, a second positioning hole 11 and a third positioning hole 12, and the first positioning hole 10, the second positioning hole 11 and the third positioning hole 12 are mutually connected.
The manufacturing method of the above embodiment adopts the following steps:
step 1: etching and exposing by a laser micro-machining technology ultraviolet laser cutting or a PDMS soft lithography technology, manufacturing a cover plate 1, an inner track panel 2 and a bottom plate 3, and placing the bottom plate 3 in a tool;
step 2: accurately bonding the inner rail panel 2 to the bottom plate 3 according to the positions of the second positioning holes 11 and the third positioning holes 12;
step 3: bonding the cover plate 1 with the inner track panel 2 according to the positions of the first positioning hole 10 and the second positioning hole 11 to complete chip packaging, and forming a complete flow channel inlet 4, 4 linear micro flow channels 5, a first flow channel outlet 8 and a second flow channel outlet 9;
step 4: and (3) rotating and bending the whole chip to form the final multi-track spiral microfluidic core.
The foregoing detailed description will set forth only for the purposes of illustrating the general principles and features of the invention, and is not meant to limit the scope of the invention in any way, but rather should be construed in view of the appended claims.
Claims (6)
1. The utility model provides a multichannel spiral micro-fluidic chip which characterized in that: including apron (1), interior track panel (2) and bottom plate (3) that stack in proper order and press from both sides the setting, apron (1), interior track panel (2) and bottom plate (3) are as a whole to be bent into heliciform structure, are provided with runner entry (4) and runner export at the both ends of interior track panel (2), are provided with a plurality of micro-channels (5) at the middle part of interior track panel (2), pass through between runner entry (4) and the runner export micro-channel (5) intercommunication each other.
2. The multi-track spiral microfluidic chip according to claim 1, wherein: the micro-channels (5) are provided with n, n is larger than 3, the micro-channels are arranged on the inner channel panel (2) in parallel, and the channel inlets (4) are communicated with the micro-channels (5) through tree-shaped bifurcation channels (6).
3. The multi-track spiral microfluidic chip according to claim 2, wherein: the flow channel outlet is provided with a partition plate (7), the partition plate (7) divides the flow channel outlet into a first flow channel outlet (8) and a second flow channel outlet (9), and the first flow channel outlet (8) and the second flow channel outlet (9) are respectively positioned on two sides of the inner track panel (2).
4. A multi-track spiral microfluidic chip according to claim 3, wherein: the cover plate (1), the inner track panel (2) and the bottom plate (3) are sequentially arranged from outside to inside on the spiral structure.
5. The multi-track spiral microfluidic chip according to claim 4, wherein: the cover plate (1), the inner track panel (2) and the bottom plate (3) are respectively provided with a first positioning hole (10), a second positioning hole (11) and a third positioning hole (12), and the first positioning holes (10), the second positioning holes (11) and the third positioning holes (12) are mutually connected.
6. A method of manufacturing a multi-track spiral microfluidic chip according to claim 4 or 5, comprising the steps of:
step 1: etching and exposing by a laser micro-machining technology ultraviolet laser cutting or a PDMS soft lithography technology, manufacturing a cover plate (1), an inner track surface plate (2) and a bottom plate (3), and placing the bottom plate (3) in a tool;
step 2: bonding the inner track panel (2) to the base plate (3);
step 3: the cover plate (1) is bonded with the inner channel panel (2) to complete chip packaging, and a complete channel inlet (4), n linear micro channels (5), a first channel outlet (8) and a second channel outlet (9) are formed;
step 4: and (3) rotating and bending the whole chip to form the final multi-track spiral microfluidic core.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210353905.1A CN114950581B (en) | 2022-04-06 | 2022-04-06 | Multi-track spiral microfluidic chip and manufacturing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210353905.1A CN114950581B (en) | 2022-04-06 | 2022-04-06 | Multi-track spiral microfluidic chip and manufacturing method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN114950581A CN114950581A (en) | 2022-08-30 |
| CN114950581B true CN114950581B (en) | 2024-02-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210353905.1A Active CN114950581B (en) | 2022-04-06 | 2022-04-06 | Multi-track spiral microfluidic chip and manufacturing method thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10106008A1 (en) * | 2000-02-11 | 2001-09-06 | Agilent Technologies Inc | Microreactor for performing polymerase chain reactions, requires only very small sample and is made of material stable during temperature cycling |
| CN103127971A (en) * | 2011-12-02 | 2013-06-05 | 国家纳米科学中心 | Micro-flow pipe and preparation method |
| CN103394384A (en) * | 2013-08-12 | 2013-11-20 | 广东顺德西安交通大学研究院 | Paper-based microfluidic chip and preparation method thereof |
| CN212417987U (en) * | 2020-06-23 | 2021-01-29 | 华北科技学院 | Liquid metal micro-fluidic mixing arrangement |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102007376B1 (en) * | 2016-11-10 | 2019-08-05 | 울산대학교 산학협력단 | A method for culturing cell using the same and a method for evaluating activity of physiologically active substances using the same |
-
2022
- 2022-04-06 CN CN202210353905.1A patent/CN114950581B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10106008A1 (en) * | 2000-02-11 | 2001-09-06 | Agilent Technologies Inc | Microreactor for performing polymerase chain reactions, requires only very small sample and is made of material stable during temperature cycling |
| CN103127971A (en) * | 2011-12-02 | 2013-06-05 | 国家纳米科学中心 | Micro-flow pipe and preparation method |
| CN103394384A (en) * | 2013-08-12 | 2013-11-20 | 广东顺德西安交通大学研究院 | Paper-based microfluidic chip and preparation method thereof |
| CN212417987U (en) * | 2020-06-23 | 2021-01-29 | 华北科技学院 | Liquid metal micro-fluidic mixing arrangement |
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| Publication number | Publication date |
|---|---|
| CN114950581A (en) | 2022-08-30 |
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