CN114949324B - Preparation method of biocompatible antibacterial gel film - Google Patents
Preparation method of biocompatible antibacterial gel film Download PDFInfo
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- CN114949324B CN114949324B CN202210677033.4A CN202210677033A CN114949324B CN 114949324 B CN114949324 B CN 114949324B CN 202210677033 A CN202210677033 A CN 202210677033A CN 114949324 B CN114949324 B CN 114949324B
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 56
- 108010025899 gelatin film Proteins 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 229920001661 Chitosan Polymers 0.000 claims abstract description 23
- 108010010803 Gelatin Proteins 0.000 claims abstract description 19
- 239000008273 gelatin Substances 0.000 claims abstract description 19
- 229920000159 gelatin Polymers 0.000 claims abstract description 19
- 235000019322 gelatine Nutrition 0.000 claims abstract description 19
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 19
- 239000002904 solvent Substances 0.000 claims abstract description 17
- 230000005496 eutectics Effects 0.000 claims abstract description 16
- 239000000499 gel Substances 0.000 claims abstract description 11
- 239000000758 substrate Substances 0.000 claims abstract description 10
- 239000011159 matrix material Substances 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 46
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 20
- 238000010438 heat treatment Methods 0.000 claims description 17
- 238000003756 stirring Methods 0.000 claims description 17
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 10
- 239000004310 lactic acid Substances 0.000 claims description 8
- 235000014655 lactic acid Nutrition 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 7
- 235000019743 Choline chloride Nutrition 0.000 claims description 7
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 7
- 229960003178 choline chloride Drugs 0.000 claims description 7
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 claims description 6
- 229960001138 acetylsalicylic acid Drugs 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims 2
- PQLVXDKIJBQVDF-UHFFFAOYSA-N acetic acid;hydrate Chemical compound O.CC(O)=O PQLVXDKIJBQVDF-UHFFFAOYSA-N 0.000 claims 1
- 239000000017 hydrogel Substances 0.000 abstract description 13
- 239000002131 composite material Substances 0.000 abstract description 4
- 239000003242 anti bacterial agent Substances 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract description 3
- 210000000416 exudates and transudate Anatomy 0.000 abstract description 3
- 239000004014 plasticizer Substances 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 7
- 230000000845 anti-microbial effect Effects 0.000 description 6
- 229960000448 lactic acid Drugs 0.000 description 6
- 230000002000 scavenging effect Effects 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 4
- 241000191967 Staphylococcus aureus Species 0.000 description 4
- 239000004599 antimicrobial Substances 0.000 description 4
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 4
- 230000002292 Radical scavenging effect Effects 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- BSYNRYMUTXBXSQ-FOQJRBATSA-N 59096-14-9 Chemical compound CC(=O)OC1=CC=CC=C1[14C](O)=O BSYNRYMUTXBXSQ-FOQJRBATSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 2
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical class CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000001804 debridement Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 239000002082 metal nanoparticle Substances 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000001550 time effect Effects 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
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- C08J5/18—Manufacture of films or sheets
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/216—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with other specific functional groups, e.g. aldehydes, ketones, phenols, quaternary phosphonium groups
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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Abstract
The invention discloses a preparation method of a biocompatible antibacterial gel film, which comprises the following steps: preparing a natural eutectic solvent; preparing gelatin solution; preparing chitosan solution; preparing an antibacterial gel solution; an antibacterial gel film was prepared. The biocompatible antibacterial gel film has good antibacterial effect and excellent mechanical property, the gelatin/chitosan composite is used as a matrix, the natural eutectic solvent with antibacterial capability is used as an antibacterial agent and a plasticizer, the hydrogel film is prepared by using the natural eutectic solvent, and the gelatin-chitosan composite substrate has excellent hydrophilic property to provide a moist tissue contact environment; has good cell compatibility and can absorb wound exudates; can be peeled off by using a hot compress mode, and is not adhered with tissues so as to avoid secondary damage caused by dressing replacement; the hydrogel film is natural and degradable, reduces the risk of environmental pollution, widens the application scene due to the excellent property of the hydrogel film, and has convenient use and low cost.
Description
Technical Field
The invention relates to the field of antibacterial gel films, in particular to a preparation method of a biocompatible antibacterial gel film.
Background
The biomedical hydrogel dressing is a good novel wound dressing, and compared with the traditional dressing, the hydrogel dressing can provide a wet healing environment, and researches show that the wound surface can heal rapidly in a moist and sealed environment. The growth of cell factors is facilitated under the sealed hypoxia condition, and the growth and healing of extracellular matrixes and blood vessels are accelerated; in a moist environment, the wound exudates can accelerate the production of various enzymes, the activity of cytokines is enhanced, necrotic cell tissues on the wound surface can be rapidly dissolved, and the debridement effect of the enzymes is enhanced; the low-oxygen environment can inhibit the generation of arachidonic acid metabolites by macrophages, and effectively improve the symptoms of local pain. The medical dressing has wide application prospect in the field of biomedical dressings, and is widely focused by researchers at home and abroad.
The antibacterial hydrogel is prepared by loading antibacterial materials on common hydrogel by a crosslinking method, a grafting method, a blending method and the like. At present, the research direction of the antibacterial hydrogel dressing mainly focuses on the antibacterial hydrogel dressing taking chitosan, polyvinyl alcohol and derivatives thereof as matrixes and taking metal nano particles such as silver, copper, zinc and the like as antibacterial agents, and the antibacterial hydrogel dressing has ideal antibacterial and bacteriostatic effects, but has the defects of complicated production process, high cost, poor mechanical property, difficult solution of environmental pollution and the like, so that the application and popularization of the antibacterial hydrogel dressing are limited.
Disclosure of Invention
In order to solve the problems of the conventional hydrogel dressing, the invention provides a preparation method of a medical hydrogel film with good antibacterial effect and excellent mechanical property, and the gel film is prepared by taking a gelatin/chitosan compound as a matrix and taking a natural eutectic solvent with antibacterial capability as an antibacterial agent and a plasticizer, wherein the excellent attribute of the gel film widens the application scene, and is convenient to use and low in cost.
The technical scheme of the invention is as follows:
a method for preparing a biocompatible antimicrobial gel film (i.e., medical antimicrobial gel film), comprising the steps of:
(1) Preparing a natural eutectic solvent: adding natural carboxylic acid (lactic acid and acetylsalicylic acid) into choline chloride, and heating and stirring to obtain uniform solution.
(2) Preparation of gelatin solution: and heating and uniformly stirring gelatin in deionized water to obtain gelatin aqueous solution.
(3) Preparing chitosan solution: and heating and uniformly stirring chitosan in an acetic acid solution (1%) to obtain a chitosan solution.
(4) Preparing a mixed substrate solution: and (3) stirring and mixing the gelatin solution and the chitosan solution according to different proportions to obtain a mixed substrate solution.
(5) Preparing an antibacterial gel solution: adding the natural eutectic solvent into the mixed base material solution according to different proportions, and uniformly stirring to obtain the antibacterial gel solution.
(6) Preparation of an antimicrobial gel film: placing the antibacterial gel solution in a matrix (such as round culture dish), and heating and drying to obtain antibacterial gel film.
In the step (1), choline chloride in the natural eutectic solvent is taken as a hydrogen bond acceptor, lactic acid and acetylsalicylic acid in the natural carboxylic acid are taken as hydrogen bond donors, and the mass ratio of the lactic acid to the acetylsalicylic acid is (1-4): (1-2): (1-2); the heating temperature is 60-80 ℃. Preferred is choline chloride: lactic acid: the mass ratio of the acetylsalicylic acid is 35:12.5:22.5 (i.e., 14:5:9), the temperature of the heating and stirring was 60 ℃.
The addition amount of gelatin relative to ionic water in the gelatin solution in the step (2) is (4-10) g/100mL, and the heating temperature is 50-70 ℃. Preferably, the addition amount is 6g/100mL, the temperature is 60 ℃, and the heating and stirring time is 1h.
The chitosan in the chitosan solution in the step (3) is added in an amount of (1-2) g/100mL relative to the acetic acid aqueous solution (the mass percentage concentration of the acetic acid aqueous solution is 0.5-2%, most preferably, the mass percentage of the acetic acid is 1%, and the solvent is deionized water), and the heating temperature is 50-70 ℃. Preferably, the addition amount is 1.5g/100mL, and the magnetic stirring is carried out at 60 ℃ for 1h.
The ratio of the gelatin solution to the chitosan solution (40-120 mL) in the step (4) is 40mL, preferably 80mL to 40mL.
The addition amount of the natural eutectic solvent in the step (5) relative to the mixed substrate solution is 1-5 g/100mL, preferably 3 g/100mL.
The antibacterial gel solution in the step (4) is placed in a culture dish, and the addition amount is 800-1500mL/m 2 The temperature is 40-60 ℃. Preferably 1000mL/m 2 Heating and drying at 50deg.C for 12 hr.
Compared with the prior art, the invention has the following advantages:
the gelatin-chitosan composite substrate used for the medical antibacterial gel film prepared by the invention has excellent hydrophilic performance and provides a moist tissue contact environment; has good cell compatibility and can absorb wound exudates; can be peeled off by using a hot compress mode, and is not adhered with tissues so as to avoid secondary damage caused by dressing replacement; is natural and degradable, and reduces the risk of environmental pollution. The gel film increases the antibacterial and antioxidant capacities of the gel film by adding the natural eutectic solvent composed of choline chloride, lactic acid and acetylsalicylic acid, and the addition of the natural eutectic solvent system can play a role in plasticizing capacity and improve the mechanical properties of the gel film; in addition, the interaction between the natural eutectic solvent and the gelatin-chitosan composite substrate can achieve the slow release effect, prolong the antibacterial time effect, and effectively reduce the drug resistance of germs by combining the external application mode.
Drawings
FIG. 1 is a schematic diagram of the antibacterial rate of an antibacterial gel film against E.coli;
FIG. 2 is a graph showing the rate of inhibition of Staphylococcus aureus by an antimicrobial gel film;
FIG. 3 is a schematic representation of the clearance of DPPH radicals from an antimicrobial gel film;
FIG. 4 is a schematic representation of the clearance of ABTS free radicals by an antimicrobial gel film.
Detailed Description
The invention will be further described with reference to the following specific examples, but the scope of the invention is not limited thereto:
example 1:
(1) Mixing 22.5g of acetylsalicylic acid, 12.5g of lactic acid and 35g of choline chloride, and stirring at 60 ℃ for 2 hours until a uniform and stable liquid, namely a natural eutectic solvent, is obtained;
(2) Adding 6g of gelatin into 100mL of deionized water, and magnetically stirring at 60 ℃ for 1h to obtain gelatin solution;
(3) Adding 1.5g of chitosan into 100mL of acetic acid solution (1%) of deionized water, and magnetically stirring at 60 ℃ for 1h to obtain a chitosan solution;
(4) Mixing 80mL of gelatin solution with 40mL of chitosan solution to obtain a mixed substrate solution;
(5) Adding 3g of natural eutectic solvent into 100mL of mixed substrate solution, and uniformly stirring at room temperature to obtain an antibacterial gel solution;
(6) According to 1000mL/m 2 And carrying out loading, casting the antibacterial gel solution on a culture dish to form a film, and heating and drying the coated culture dish at 50 ℃ for 12 hours to obtain the antibacterial gel film.
The experimental results are shown in fig. 1 and 2. Fig. 1 and 2 show the antibacterial rate of the antibacterial gel film and the common gelatin film on escherichia coli and staphylococcus aureus, and fig. 1 and 2 show that the common gelatin film has no antibacterial effect on escherichia coli and staphylococcus aureus, and the antibacterial gel film has obvious antibacterial effect, and the antibacterial rate on escherichia coli and staphylococcus aureus reaches more than 98%.
(6) The obtained antibacterial gel film was cut into a suitable size, and the antioxidant effect of the antibacterial gel film and the common gelatin film was examined using the DPPH radical scavenging method.
FIG. 3 shows DPPH radical scavenging by antibacterial gel film and ordinary gelatin film, and FIG. 4 shows ABTS radical scavenging by antibacterial gel film and ordinary gelatin film.
As shown in FIG. 3, the common gelatin film has little effect of scavenging DPPH free radicals, while the antibacterial gel film has obvious effect of scavenging DPPH free radicals, and the scavenging rate of the antibacterial gel film reaches more than 58 percent. As can be seen from FIG. 4, the antibacterial gel film has a remarkable effect of scavenging ABTS free radicals, and the scavenging rate of the ABTS free radicals reaches more than 32% compared with the common gelatin film. The antibacterial gel film has obvious antioxidation effect.
Claims (6)
1. The preparation method of the biocompatible antibacterial gel film is characterized by comprising the following steps of:
(1) Lactic acid and acetylsalicylic acid are added into choline chloride, and the choline chloride: lactic acid: the mass ratio of the acetylsalicylic acid is (1-4): (1-2): (1-2) heating and stirring the mixture at 60-80 ℃ to fully mix the uniform solution, thereby obtaining the natural eutectic solvent;
(2) Heating gelatin in deionized water and stirring uniformly to obtain gelatin water solution;
(3) Heating chitosan in acetic acid water solution, and stirring uniformly to obtain chitosan solution;
(4) Mixing gelatin solution and chitosan solution according to different proportions to obtain mixed substrate solution;
(5) Adding natural eutectic solvent into the mixed base material solution according to different proportions, and uniformly stirring to obtain an antibacterial gel solution;
the addition amount of the natural eutectic solvent relative to the mixed substrate solution is 1-5 g/100mL;
(6) Placing the antibacterial gel solution into a matrix, and heating and drying to obtain an antibacterial gel film;
the addition amount of the antibacterial gel solution is 800-1500mL/m 2 The temperature is 40-60 ℃.
2. The method for preparing a biocompatible antibacterial gel film according to claim 1, wherein in the step (2), the addition amount of gelatin in the gelatin solution relative to deionized water is (4-10) g/100mL.
3. The method for preparing a biocompatible antibacterial gel film according to claim 1, wherein in the step (2), the temperature of heating and stirring is 50-70 ℃.
4. The method for preparing a biocompatible antibacterial gel film according to claim 1, wherein in the step (3), the addition amount of chitosan in the chitosan solution relative to the aqueous acetic acid solution is (1-2) g/100mL;
the mass percentage concentration of the acetic acid aqueous solution is 0.5-2%.
5. The method for preparing a biocompatible antibacterial gel film according to claim 1, wherein in the step (3), the temperature of heating and stirring is 50-70 ℃.
6. The method for preparing a biocompatible antibacterial gel film according to claim 1, wherein in the step (4), the ratio of the gelatin solution to the chitosan solution is 40-120 mL.
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