CN114946855A - Preparation method and application of tomato sub-low temperature resistance-inducing/resistance-increasing agent - Google Patents
Preparation method and application of tomato sub-low temperature resistance-inducing/resistance-increasing agent Download PDFInfo
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Abstract
The invention discloses a preparation method and application of a tomato sub-low temperature resistance-inducing/resistance-increasing agent, and relates to the technical field of vegetable planting, wherein the sub-low temperature resistance-inducing/resistance-increasing agent is prepared from ALA (5-aminolevulinic acid), oligosaccharide and/or CaCl with different concentrations 2 The composition of the calcium chloride is applied to the production process of facility tomatoes in winter and spring in low temperature seasons, can obviously enhance the resistance of tomato plants under the sub-low temperature condition, promotes the normal flowering, fruit setting and fruit development of the tomato plants, and improves the tomato yield under the sub-low temperature environmentAmount and quality.
Description
Technical Field
The invention relates to a composition formula for enhancing the sub-low temperature resistance of crops and application thereof, in particular to a preparation method of a tomato sub-low temperature resistance inducing/increasing agent and application thereof.
Background
Low temperature stress (cold injury and freeze injury) refers to a temperature environment lower than the lower limit of the optimal growth temperature of plants, and is an important environmental limiting factor influencing the growth, development and geographical distribution of plants. The low temperature stress affects the growth of plants and the yield and quality of crops, resulting in the yield reduction of horticultural crops such as facility vegetables, and becomes an urgent problem to be solved in actual production. The low temperature is the most important adversity obstacle affecting vegetable production in facilities in winter and spring, and the temperature in the facilities is lower than 20 ℃ in the daytime and is 6-12 ℃ or lower in the nighttime, namely the condition of sub-low temperature is often generated. When vegetable crops are in a sub-low temperature environment, physiological activity disorder, photosynthesis blockage, growth slowing and dry matter accumulation reduction are caused, and finally, the yield and the quality are seriously reduced.
The functions of the existing plant resistance inducer are mainly concentrated on plant growth promotion or pest and disease immunity, single ingredients such as salicylic acid are mostly used as core substances in the sub-low temperature or low temperature resistance increasing technology, and 5-aminolevulinic acid (ALA), oligosaccharide and calcium chloride (CaCl) are utilized to improve plant resistance, promote plant growth and improve physiological metabolism level, yield and quality of plants under the condition of not aiming at the sub-low temperature 2 ) The developed sub-low temperature synergistic inducer/resistance increasing agent technology. ALA, oligosaccharide and CaCl under the conditions of low temperature and sub-low temperature 2 Based on the effects of inducing resistance and increasing resistance to tomatoes when combined at different concentrations, ALA, oligosaccharide and CaCl are used in the invention 2 A sub-low temperature induced resistance/resistance increasing agent is developed as a material, so that the resistance of a big fruit type tomato plant under the sub-low temperature condition can be obviously improved, and the quality and the yield are improved.
Disclosure of Invention
The invention aims to provide a preparation method and application of a tomato sub-low temperature resistance inducer, so as to solve the problems in the background technology. The invention provides a preparation method of 5-aminolevulinic acid(ALA), oligosaccharide and calcium chloride (CaCl) 2 ) The resistance inducing/resistance increasing agent is a sub-low temperature inducing/resistance increasing agent of the material, and can obviously improve the resistance of tomato plants, improve the physiological metabolism level of the plants and improve the yield and quality of facility tomatoes in sub-low temperature seasons by spraying the resistance inducing/resistance increasing agent before or on time when the sub-low temperature comes.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a preparation method of a tomato sub-low temperature resistance inducing/increasing agent comprises the following steps of:
20. 30, 50 and 60mg/L ALA: dissolving 10g of ALA powder into 100mL of water at normal temperature in a dark place to prepare ALA mother liquor, respectively adding 200, 300, 500 and 600 mu L of mother liquor into 1L of water, shaking up to obtain the product, and storing in the dark place;
75. 150 and 225mg/L oligosaccharide: respectively dissolving 75, 150 and 225mg of oligosaccharide into 1L of water, and storing in dark place;
20 and 30nmol/L CaCl 2 : 2.22 and 3.33g of CaCl were weighed out separately 2 (molecular weight 110.98), or 2.94 and 4.41g CaCl 2 ·2H 2 O (molecular weight 147.0146), respectively dissolving in 1L water to obtain the final product;
the formulation of the sub-low temperature inducer/booster is as follows:
10mg/L ALA +75mg/L oligosaccharide +10nmol/L CaCl 2 :30 mg/L ALA, 225mg/L oligosaccharide and 30nmol/L CaCl 2 The three mother solutions are weighed according to the equal volume, mixed and shaken evenly, and are stored in a dark place;
10mg/LALA+10nmol/L CaCl 2 : 20mg/L ALA and 20nmol/L CaCl 2 Mixing the two mother solutions according to the equal volume, shaking up, and storing in dark place;
25mg/L ALA +75mg/L oligosaccharide: weighing two mother solutions of 50mg/L ALA and 150mg/L oligosaccharide according to equal volume, mixing, and shaking to obtain the final product, which is stored in dark place;
20mg/L ALA +50mg/L oligosaccharide +10nmol/L CaCl 2 : 60mg/L ALA, 150mg/L oligosaccharide and 30nmol/L CaCl 2 The three mother solutions are weighed according to the equal volume, mixed and shaken up to obtain the product, and the product is stored in a dark place.
As a further scheme of the invention: also included are the sub-hypothermia inducer/booster types and application times, which are as follows:
sub-low temperature inducer 1: 10mg/L ALA +75mg/L oligosaccharide +10nmol/L CaCl 2 Pre-spraying once 6-18h before the coming of the sublow temperature;
sub-low temperature inducer 2: 10mg/L ALA +10nmol/L CaCl 2 Pre-spraying once in 10-16h before the coming of the sublow temperature;
sub-low temperature inducer 3: 25mg/L ALA +75mg/L oligosaccharide, pre-spraying once before the sub-low temperature comes for 8-16h, and spraying once again when the sub-low temperature lasts for the 6 th day;
sub-low temperature resistance increasing agent 1: 20mg/L ALA +50mg/L oligosaccharide +10nmol/L CaCl 2 The spraying agent is sprayed once at a sub-low temperature.
Preferably, the sub-low temperature resistance inducer 1 is sprayed once 8 hours before the prediction of the coming of the sub-low temperature, and is matched with the sub-low temperature resistance increasing agent 1 which is sprayed once in the coming of the sub-low temperature.
Preferably, the sub-low temperature resistance inducer 2 is sprayed 12 hours before the prediction of the coming of the sub-low temperature, and is matched with the sub-low temperature resistance increasing agent 1 which is sprayed once in the coming of the sub-low temperature.
Preferably, the sub-low temperature resistance inducer 3 is sprayed 12 hours before the sub-low temperature comes, and is sprayed again when the sub-low temperature continues for the 6 th day.
Preferably, the sub-low temperature resistance increasing agent 1 is temporarily and immediately sprayed for 1 time at the sub-low temperature, and is additionally sprayed for 1 time when the sub-low temperature continuously lasts for 8 hours.
As a further scheme of the invention: the method also comprises an application mode which comprises the following steps:
the sub-low temperature resistance inducing/resistance increasing agent is applied in a mode of spraying on the leaf surfaces before the sub-low temperature comes, and the sprayed plant parts are the front and back surfaces of leaves at each leaf position of the tomatoes.
As a further scheme of the invention: when the low-temperature resistance-inducing/resistance-increasing agent is applied, the spraying dosage of the low-temperature resistance-inducing/resistance-increasing agent is that the front and back surfaces of the leaves are uniformly adhered and no liquid drops drop, 6-12 mL/plant is sprayed on each tomato seedling stage (3 leaves from 1 heart to 1 inflorescence flowering period), and 15-20 mL/plant is sprayed after the tomatoes enter a fruit setting stage (1 inflorescence flowering period).
Preferably, 8mL of the tomato seedlings are sprayed per plant, and 18mL of the tomato seedlings are sprayed per plant after the tomatoes enter the fruit setting period.
As a further scheme of the invention: when in application, the sub-low temperature inducing-resisting/resisting agent is suitable for large fruit type tomato varieties, the sub-low temperature adverse environment temperature conditions of the tomato are that the temperature range is 8-20 ℃ in the daytime, the temperature range is 6-12 ℃ at night, and the temperature naturally gradually changes all day long.
As a further scheme of the invention: in the sub-low temperature inducer/synergist, the sub-low temperature synergist 1 and any one of the sub-low temperature inducers 1, 2 or 3 can be applied in a matched mode according to the application time before the sub-low temperature comes, the sub-low temperature resistance of the tomatoes is enhanced in a synergistic mode, but the sub-low temperature inducers 1, 2 and 3 cannot be mixed and matched or used repeatedly before the sub-low temperature comes.
As a further scheme of the invention: the sub-low temperature resistance inducing/resistance increasing agent is applied in the time period of avoiding strong light irradiation in sunny days of 16:30-18:30 or at night, and the spraying time in cloudy days is not specifically specified.
Preferably, the sub-low temperature inducer/synergist is sprayed after sunset.
Compared with the prior art, the invention has the beneficial effects that:
(1) the sub-low temperature induced resistance/resistance increasing agent is applied to the production process of facility tomatoes under the sub-low temperature condition, can improve the resistance of the tomatoes to the sub-low temperature stress, improve the oxidation resistance and physiological metabolism level of plants, promote the absorption, transportation, assimilation and utilization of nutrient elements of the facility tomato plants, and promote the growth of the plants and the normal development of fruits under the sub-low temperature condition;
(2) the sub-low temperature induced resistance/resistance increasing agent is applied to the production process of facility tomatoes under the sub-low temperature condition, can efficiently induce or increase the resistance of plants to the sub-low temperature by one-time application, has small using amount, small workload and excellent effect, can effectively overcome the damage of the sub-low temperature environment to the plants, and continuously ensures the normal growth, flowering and fruit production of the plants.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the embodiment of the invention, the preparation method of the sub-low temperature inducer/synergist comprises the following steps of:
20. 30, 50 and 60mg/L ALA: dissolving 10g of ALA powder into 100mL of water at normal temperature in a dark place to prepare ALA mother liquor, respectively adding 200, 300, 500 and 600 mu L of mother liquor into 1L of water, shaking up to obtain the product, and storing in the dark place;
75. 150 and 225mg/L oligosaccharide: respectively dissolving 75, 150 and 225mg of oligosaccharide into 1L of water, and storing in dark place;
20 and 30nmol/L CaCl 2 : 2.22 and 3.33g of CaCl were weighed out separately 2 (molecular weight 110.98), or 2.94 and 4.41g CaCl 2 ·2H 2 O (molecular weight 147.0146), respectively dissolving in 1L water.
The formulation of the sub-low temperature inducer/booster is as follows:
sub-low temperature inducer 1: 10mg/L ALA +75mg/L oligosaccharide +10nmol/L CaCl 2 :30 mg/L ALA, 225mg/L oligosaccharide and 30nmol/L CaCl 2 The three mother solutions are weighed according to the equal volume, mixed and shaken evenly, and are stored in a dark place;
sub-low temperature inducer 2: 10mg/LALA +10nmol/L CaCl 2 : 20mg/L ALA and 20nmol/L CaCl 2 Mixing the two mother solutions according to the equal volume, shaking up, and storing in dark place;
sub-low temperature inducer 3: 25mg/L ALA +75mg/L oligosaccharide: weighing two mother solutions of 50mg/L ALA and 150mg/L oligosaccharide according to equal volume, mixing, and shaking to obtain the final product, which is stored in dark place;
sub-low temperature resistance increasing agent 1: 20mg/L ALA +50mg/L oligosaccharide +10nmol/L CaCl 2 : 60mg/L ALA, 150mg/L oligosaccharide and 30nmol/L CaCl 2 The three mother solutions are weighed according to the equal volume, mixed and shaken up to obtain the product, and the product is stored in a dark place.
Tomato varieties to be tested: a sub-low temperature sensitive tomato variety 'Jinpeng No. 1' and a conventional cultivation variety 'Kaideya';
seedling culture conditions are as follows: culturing at normal temperature at 22-28 deg.C in daytime and 17-19 deg.C at night;
transplanting seedlings: transplanting the seedlings to sunlight greenhouse soil when the seedlings grow to 3 leaves and 1 heart, and performing a seedling stage test or a fruiting stage test;
and (3) sub-low temperature control: the indoor temperature is regulated and controlled by adopting an electric warm air warming fan and a refrigeration air conditioner;
sub-low temperature conditions: the temperature during the sub-low temperature test is 8-12 ℃ in the daytime and 6-8 ℃ at night.
The application of the preparation method of the sub-low temperature inducer/synergist is as follows:
example 1
Tomato seedling stage test: when the tomatoes grow to 5 leaves and 1 heart under the conventional temperature condition (25-28 ℃ in the daytime and 17-20 ℃ in the nighttime), spraying of a sub-low temperature resistance inducing/resistance increasing agent and sub-low temperature treatment are carried out, and the specific implementation components and modes are as follows:
group A test: spraying a sub-low temperature resistance inducer 1 on the tomato leaf surfaces, controlling a sub-low temperature natural temperature change interval of 8-12 ℃ in the daytime and 6-8 ℃ at night by using a temperature increasing and reducing device after spraying for 12h, wherein the spraying dosage is 8 mL/plant;
group B test: spraying a sub-low temperature resistance inducer 2 on the tomato leaf surfaces, controlling a sub-low temperature natural temperature change interval of 8-12 ℃ in the daytime and 6-8 ℃ at night by using a temperature increasing and reducing device after spraying for 12h, wherein the spraying dosage is 8 mL/plant;
group C tests: spraying a sub-low temperature resistance inducer 3 on the tomato leaf surfaces, controlling a sub-low temperature natural temperature change interval of 8-12 ℃ in the daytime and 6-8 ℃ at night by using a temperature increasing and reducing device after spraying for 12h, wherein the spraying dosage is 8 mL/plant;
and D, group test: controlling a sub-low temperature natural temperature change range with the daytime temperature of 8-12 ℃ and the nighttime temperature of 6-8 ℃ by using a temperature increasing and reducing device, immediately spraying 1 time of a sub-low temperature resistance increasing agent 1 after the temperature is in the sub-low temperature, and additionally spraying 1 time when the sub-low temperature lasts for 8 hours, wherein the spraying dosage is 8 mL/plant;
low temperature control test: spraying clear water to the leaf surfaces of the tomatoes when the tomatoes grow to 5 leaves and 1 heart according to the temperature conditions in the group A, controlling a sub-low-temperature natural variable temperature range of 8-12 ℃ in the daytime and 6-8 ℃ at night by using a temperature increasing and reducing device after spraying for 12h, wherein the spraying dosage is 8mL per plant;
normal temperature control test: when the tomatoes grow to 5 leaves and 1 heart according to the temperature conditions of the group A, spraying clear water on the surfaces of the tomato leaves, and culturing at normal temperature without performing sub-low temperature treatment after spraying;
and (3) sampling at 7d after the beginning of the sub-low temperature in the seedling stage test, and determining low-temperature resistance indexes such as chlorophyll content, chlorophyll fluorescence parameter PS II maximum photochemical quantum yield (Fv/Fm), relative electrolyte permeability, Malondialdehyde (MDA) and the like in plant leaves.
Example 2
Test on the result period: when the tomato grows to the 1 st ear of fruit to turn color under the conventional temperature condition (25-28 ℃ in the daytime and 17-20 ℃ in the nighttime), the test treatment is carried out:
group A test: spraying a sub-low temperature resistance inducer 1 on the tomato leaf surfaces, controlling a sub-low temperature natural temperature change interval of 8-12 ℃ in the daytime and 6-8 ℃ at night by using a temperature increasing and reducing device after spraying for 12h, wherein the spraying dosage is 18 mL/plant;
group B test: spraying a sub-low temperature resistance inducer 2 on the tomato leaf surfaces, controlling a sub-low temperature natural temperature change interval of 8-12 ℃ in the daytime and 6-8 ℃ at night by using a temperature increasing and reducing device after spraying for 12h, wherein the spraying dosage is 18 mL/plant;
group C test: spraying a sub-low temperature resistance inducer 3 on the tomato leaf surfaces, controlling a sub-low temperature natural temperature changing range with the daytime temperature of 8-12 ℃ and the nighttime temperature of 6-8 ℃ by using a temperature increasing and reducing device after spraying for 12h, and performing supplementary spraying for 1 time after entering the sub-low temperature range for 6h, wherein the spraying doses are respectively 18 mL/plant and 15 mL/plant; (ii) a
And D, group test: controlling a sub-low temperature natural temperature change range with the daytime temperature of 8-12 ℃ and the nighttime temperature of 6-8 ℃ by using a temperature increasing and reducing device, immediately spraying 1 time of a sub-low temperature resistance increasing agent 1 after the temperature is in the sub-low temperature, and additionally spraying 1 time when the sub-low temperature lasts for 8 hours, wherein the spraying dosage is 18 mL/plant;
control test: spraying clear water on tomato leaves, controlling a sub-low temperature natural variable temperature interval of 8-12 ℃ in daytime and 6-8 ℃ at night by using a temperature increasing and reducing device after spraying for 12h, wherein the spraying dosage is 18 mL/plant;
normal temperature control test: spraying clear water on the leaves of the tomatoes, carrying out no sub-low temperature treatment after spraying, and carrying out normal temperature condition culture all the time, wherein the spraying dosage is 18 mL/plant;
and (3) simulating a natural sub-low temperature change rule to control the temperature in a result period test, recording the fruit yield in the test period, sampling until the 3 rd ear fruit turns to be colored and ripe, and measuring indexes such as the fruit yield and the fruit quality.
The test of each group is respectively provided with 3 repeated groups, each group is provided with 50 repeated plants, when each index is measured, 20 plants are randomly selected for measurement, and the effect of the sub-low temperature resistance inducing/resisting agent is represented according to the average value of the low temperature resistance index, the yield, the quality and other indexes of each group test treatment, which is specifically as follows:
TABLE 1 measurement of the Effect of sub-hypothermia elicitor/booster on chlorophyll content and Fv/Fm in tomato seedlings
According to the test results in table 1, the chlorophyll content and Fv/Fm of the plants in the sub-low temperature control are both significantly smaller than those in the normal temperature control, which indicates that under the sub-low temperature condition, the chlorophyll content and the maximum photochemical quantum yield of the psii of the plants are significantly reduced, and significant influence is exerted on the photosynthetic parameters of the plants, after the sub-low temperature resistance inducing/resistance increasing agent disclosed by the invention is sprayed, the chlorophyll content and Fv/Fm of the seedlings are both significantly higher than those in the sub-low temperature control treatment, after the sub-low temperature resistance inducing agent 2 and 3 are treated, the total chlorophyll amount of the seedlings of the two varieties is not significantly different from that in the normal temperature control, and after the sub-low temperature resistance inducing agent 3 and the resistance increasing agent 1 are treated, the Fv/Fm values of the two varieties are not significantly different from that in the normal temperature control treatment. The test result shows that under the condition of 1 time of spraying, the sub-low temperature resistance inducers 1, 2 and 3 and the sub-low temperature resistance increaser 1 obviously reduce the damage of the sub-low temperature environment to photosynthesis.
TABLE 2 measurement of the Effect of sub-hypothermia elicitor/booster on the accumulation of Biomass in tomato seedlings
According to the test results in table 2, after the treatment at the sub-low temperature for 7d, the fresh and dry matter accumulation of the tomato seedlings is significantly reduced, but compared with the sub-low temperature control, the fresh and dry matter accumulation of the overground and root systems of the seedling plants of two tomato varieties is significantly increased after the three sub-low temperature resistance inducers and the resistance increasing agents are pretreated before the sub-low temperature, and the fresh quality of the overground and root systems and the dry quality of the overground and root systems of the plants after the treatment with the sub-low temperature resistance inducers 2 (group B) and 3 (group C) have no significant difference from the normal temperature control treatment, which shows that under the condition of 1-time spraying, the sub-low temperature resistance inducers 1, 2, 3 and the sub-low temperature resistance increasing agent 1 have significant promotion effects on the growth and biomass accumulation of the tomato plants under the sub-low temperature environment.
TABLE 3 measurement of the Effect of sub-hypothermia inducer/synergist on the accumulation of tomato seedling Biomass
The relative electrolyte leakage rate of leaves and the MDA content are used as key indexes for representing the stress degree of the plants, and the electrolyte leakage rate of the leaves and the MDA content of the plants can be obviously increased under the condition of subambient temperature stress. As shown in Table 3, the relative electrolyte leakage rate and MDA content of leaves of two varieties are obviously increased under the sub-low temperature environment, and in the plants to which the sub-low temperature inducing resistance/resistance increasing agents are pre-sprayed, two indexes are obviously reduced compared with those of the plants subjected to low temperature control, so that the difference between the indexes and the physiological state of the plants under the normal temperature cultivation condition is reduced, and the test result shows that the stress damage degree of the sub-low temperature inducing resistance increasing agents disclosed by the invention on the physiological metabolism of the plants under the sub-low temperature environment can be obviously reduced by pre-spraying.
TABLE 4 results of the determination of the effect of sub-low temperature inducer/booster on tomato yield
As can be seen from Table 4, the continuous exposure to the sub-low temperature environment significantly reduces the individual plant yield of different tomato varieties, but compared with the sub-low temperature control, the leaf surface spraying of the sub-low temperature resistance-inducing/resistance-increasing agent disclosed by the invention significantly improves the individual plant yield of the tomato plants. The test result shows that the sub-low temperature induced resistance/resistance increasing agent has obvious effect on promoting the effect of protecting the harvest and the yield of the tomatoes which are arranged under the condition of the sub-low temperature environment.
TABLE 5 Effect of sub-hypothermia inducer/booster on tomato quality
According to the fruit quality determination results in table 5, the content of vitamin c (vc), free amino acids, reducing sugars, soluble sugars and organic acids in the tomato fruits is significantly reduced under the condition of the sub-low temperature environment, the fruit quality under the sub-low temperature environment is significantly improved by spraying the sub-low temperature resistance-inducing/resistance-increasing agent on the leaf surfaces, and the content of the reducing sugars, the soluble sugars and the organic acids in the fruits is even higher than that of the normal temperature control treatment, so that under the condition of the sub-low temperature environment, the sub-low temperature resistance-inducing/resistance-increasing agent provided by the invention not only can significantly improve the tomato yield, but also has a significant improvement effect on the tomato fruit quality under the condition of the sub-low temperature environment.
Meanwhile, the sub-low temperature induced resistance/resistance increasing agent is further subjected to application effect verification, and test results show that for the tomato in the seedling stage, the induced resistance technology can at least ensure higher resistance of tomato plants within 25 days of continuous sub-low temperature, and the morphological growth vigor, biomass and stress resistance of the tomato plants are remarkably improved compared with the plants which grow under the pure sub-low temperature without the technology; at low temperature, the resistance inducing technology can relieve the reduction trend of the accumulation amount of N, P, K elements in different organs of tomatoes caused by sub-low temperature, can promote the accumulation of Ca in roots, stems and leaves of the tomatoes, and promotes the accumulation of Mg in stems and leaves and the accumulation of Fe in overground parts. After the induction technology is applied, the tomato plants subjected to the sublow temperature stress grow better in the recovery period, the recovery condition of each index is better, and compared with the plants which grow under the simple sublow temperature without the application of the technology, the induction technology is obviously improved, so that the growth vigor, the biomass and the like of the plants can be recovered to be normal.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (7)
1. The preparation method of the tomato sub-low temperature resistance inducing/increasing agent is characterized in that the preparation method of the mother solution is as follows:
20. 30, 50 and 60mg/L ALA: dissolving 10g of ALA powder into 100mL of water at normal temperature in a dark place to prepare ALA mother liquor, respectively adding 200, 300, 500 and 600 mu L of mother liquor into 1L of water, shaking up to obtain the product, and storing in the dark place;
75. 150 and 225mg/L oligosaccharide: respectively dissolving 75, 100 and 225mg of oligosaccharide into 1L of water, and storing in dark place;
20 and 30nmol/L CaCl 2 : 2.22 and 3.33g of CaCl were weighed out separately 2 (molecular weight 110.98), or 2.94 and 4.41g CaCl 2 ·2H 2 O (molecular weight 147.0146), respectively dissolving in 1L water to obtain the final product;
10mg/L ALA +75mg/L oligosaccharide +10nmol/L CaCl 2 :30 mg/L ALA, 225mg/L oligosaccharide and 30nmol/L CaCl 2 The three mother solutions are weighed according to the equal volume, mixed and shaken evenly, and are stored in a dark place;
10mg/L ALA+10nmol/L CaCl 2 : 20mg/L ALA and 20nmol/L CaCl 2 Mixing the two mother solutions according to the equal volume, shaking up, and storing in dark place;
25mg/L ALA +75mg/L oligosaccharide: weighing two mother solutions of 50mg/L ALA and 150mg/L oligosaccharide according to equal volume, mixing, and shaking to obtain the final product, which is stored in dark place;
20mg/L ALA +50mg/L oligosaccharide +10nmol/L CaCl 2 : 60mg/L ALA, 150mg/L oligosaccharide and 30nmol/L CaCl 2 The three mother solutions are weighed according to the equal volume, mixed and shaken up to obtain the product, and the product is stored in a dark place.
2. The method of formulating tomato sub-low temperature inducer/potentiator according to claim 1, wherein the sub-low temperature inducer/potentiator type and application time are as follows:
sub-low temperature inducer 1: 10mg/L ALA +75mg/L oligosaccharide +10nmol/L CaCl 2 Pre-spraying once 6-18h before the coming of the sublow temperature;
sub-low temperature inducer 2: 10mg/L ALA +10nmol/L CaCl 2 Pre-spraying once in 10-16h before the coming of the sublow temperature;
sub-low temperature inducer 3: 25mg/L ALA +75mg/L oligosaccharide, pre-spraying once before the sub-low temperature comes for 8-16h, and spraying once again when the sub-low temperature lasts for the 6 th day;
sub-low temperature resistance increasing agent 1: 20mg/L ALA +50mg/L oligosaccharide +10nmol/L CaCl 2 The spraying agent is sprayed once at a sub-low temperature.
3. The use of the tomato sub-low temperature inducer/synergist in the preparation method of claim 1, wherein the sub-low temperature inducer/synergist is applied by foliar spray, and the sprayed plant parts are the front and back surfaces of each leaf position of the tomato.
4. The application of the preparation method of the tomato sub-low temperature resistance-inducing/resistance-increasing agent as claimed in any one of claims 1-3, wherein the spraying dosage of the sub-low temperature resistance-inducing/resistance-increasing agent is that the front and back surfaces of the tomato leaves are uniformly adhered without dripping, 6-12 mL/plant is sprayed on the tomato seedling (from 1 heart of 3 leaves to 1 inflorescence blooming period), and 15-20 mL/plant is sprayed after the tomato enters the fruit setting period (after the 1 inflorescence blooms).
5. The use of the method of formulating a tomato sub-low temperature inducer/synergist as claimed in any one of claims 1-2, wherein said sub-low temperature inducer/synergist is suitable for use in large fruit tomato varieties, and the conditions of sub-low temperature stress in tomato are 8-20 ℃ in the daytime, 6-12 ℃ in the nighttime, and the temperature changes naturally throughout the day.
6. The use of the method for formulating tomato sub-low temperature inducer/potentiator according to any one of claims 1-2, wherein in the sub-low temperature inducer/potentiator, sub-low temperature potentiator 1 can be administered in combination with one of sub-low temperature inducer 1, 2, or 3, optionally at each time of administration before the onset of sub-low temperature, to synergistically enhance the sub-low temperature resistance of tomato, but sub-low temperature inducer 1, 2, or 3 cannot be mixed and matched or reused before the onset of sub-low temperature.
7. The use of the tomato sub-low temperature inducer according to any one of claims 1-2, characterized in that the sub-low temperature inducer/synergist is applied avoiding the period of intense light irradiation, 16:30-18:30 in sunny days or at night, and the spraying time in cloudy days is not specifically defined.
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