CN114945670A - 一种碱基编辑***和其使用方法 - Google Patents
一种碱基编辑***和其使用方法 Download PDFInfo
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- CN114945670A CN114945670A CN202080059623.XA CN202080059623A CN114945670A CN 114945670 A CN114945670 A CN 114945670A CN 202080059623 A CN202080059623 A CN 202080059623A CN 114945670 A CN114945670 A CN 114945670A
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Abstract
提供了一种碱基编辑融合蛋白,该碱基编辑融合蛋白包含核酸靶向结构域、胞嘧啶脱氨酶结构域和腺嘌呤脱氨酶结构域。还提供了一种碱基编辑***和产生至少一个经遗传修饰的细胞的方法。
Description
PCT国内申请,说明书已公开。
Claims (26)
- PCT国内申请,权利要求书已公开。
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CN2019107674188 | 2019-08-20 | ||
PCT/CN2020/110207 WO2021032155A1 (zh) | 2019-08-20 | 2020-08-20 | 一种碱基编辑***和其使用方法 |
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CN115820603A (zh) * | 2022-11-15 | 2023-03-21 | 吉林大学 | 一种基于dCasRx-NSUN6单基因特异性M5C修饰编辑方法 |
CN116751799A (zh) * | 2023-06-14 | 2023-09-15 | 江南大学 | 一种多位点双重碱基编辑器及其应用 |
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CN113201517B (zh) * | 2021-05-12 | 2022-11-01 | 广州大学 | 一种胞嘧啶单碱基编辑器工具及其应用 |
CN115704015A (zh) * | 2021-08-12 | 2023-02-17 | 清华大学 | 基于腺嘌呤和胞嘧啶双碱基编辑器的靶向诱变*** |
CN117683763A (zh) * | 2022-09-09 | 2024-03-12 | 中国科学院遗传与发育生物学研究所 | 基于dna聚合酶的基因组编辑***和方法 |
CN118086285B (zh) * | 2024-04-23 | 2024-07-19 | 天津凯莱英生物科技有限公司 | 蛋白定向进化的方法 |
Citations (4)
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CN109517841A (zh) * | 2018-12-05 | 2019-03-26 | 华东师范大学 | 一种用于核苷酸序列修饰的组合物、方法与应用 |
CN109957569A (zh) * | 2017-12-22 | 2019-07-02 | 中国科学院遗传与发育生物学研究所 | 基于cpf1蛋白的碱基编辑***和方法 |
WO2019147014A1 (ko) * | 2018-01-23 | 2019-08-01 | 기초과학연구원 | 연장된 단일 가이드 rna 및 그 용도 |
CN110835634A (zh) * | 2018-08-15 | 2020-02-25 | 华东师范大学 | 一种新型碱基转换编辑***及其应用 |
-
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- 2020-08-20 WO PCT/CN2020/110207 patent/WO2021032155A1/zh active Application Filing
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CN109957569A (zh) * | 2017-12-22 | 2019-07-02 | 中国科学院遗传与发育生物学研究所 | 基于cpf1蛋白的碱基编辑***和方法 |
WO2019147014A1 (ko) * | 2018-01-23 | 2019-08-01 | 기초과학연구원 | 연장된 단일 가이드 rna 및 그 용도 |
CN110835634A (zh) * | 2018-08-15 | 2020-02-25 | 华东师范大学 | 一种新型碱基转换编辑***及其应用 |
CN109517841A (zh) * | 2018-12-05 | 2019-03-26 | 华东师范大学 | 一种用于核苷酸序列修饰的组合物、方法与应用 |
Non-Patent Citations (2)
Title |
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CHAO LI 等: "Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion", GENOME BIOLOGY, vol. 19, no. 1, pages 1, XP055655088, DOI: 10.1186/s13059-018-1443-z * |
NICOLE M. GAUDELLI ET AL.: "Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage", NATURE, no. 551, 25 October 2017 (2017-10-25), pages 464 - 471 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115820603A (zh) * | 2022-11-15 | 2023-03-21 | 吉林大学 | 一种基于dCasRx-NSUN6单基因特异性M5C修饰编辑方法 |
CN116751799A (zh) * | 2023-06-14 | 2023-09-15 | 江南大学 | 一种多位点双重碱基编辑器及其应用 |
CN116751799B (zh) * | 2023-06-14 | 2024-01-26 | 江南大学 | 一种多位点双重碱基编辑器及其应用 |
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