CN114945670A - 一种碱基编辑***和其使用方法 - Google Patents
一种碱基编辑***和其使用方法 Download PDFInfo
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- CN114945670A CN114945670A CN202080059623.XA CN202080059623A CN114945670A CN 114945670 A CN114945670 A CN 114945670A CN 202080059623 A CN202080059623 A CN 202080059623A CN 114945670 A CN114945670 A CN 114945670A
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Abstract
提供了一种碱基编辑融合蛋白，该碱基编辑融合蛋白包含核酸靶向结构域、胞嘧啶脱氨酶结构域和腺嘌呤脱氨酶结构域。还提供了一种碱基编辑***和产生至少一个经遗传修饰的细胞的方法。
Description
PCT国内申请，说明书已公开。
Claims (26)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910767418 | 2019-08-20 | ||
| CN2019107674188 | 2019-08-20 | ||
| PCT/CN2020/110207 WO2021032155A1 (zh) | 2019-08-20 | 2020-08-20 | 一种碱基编辑***和其使用方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114945670A true CN114945670A (zh) | 2022-08-26 |
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| CN202080059623.XA Pending CN114945670A (zh) | 2019-08-20 | 2020-08-20 | 一种碱基编辑***和其使用方法 |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN114945670A (zh) |
| WO (1) | WO2021032155A1 (zh) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115820603A (zh) * | 2022-11-15 | 2023-03-21 | 吉林大学 | 一种基于dCasRx-NSUN6单基因特异性M5C修饰编辑方法 |
| CN116751799A (zh) * | 2023-06-14 | 2023-09-15 | 江南大学 | 一种多位点双重碱基编辑器及其应用 |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113201517B (zh) * | 2021-05-12 | 2022-11-01 | 广州大学 | 一种胞嘧啶单碱基编辑器工具及其应用 |
| CN115704015A (zh) * | 2021-08-12 | 2023-02-17 | 清华大学 | 基于腺嘌呤和胞嘧啶双碱基编辑器的靶向诱变*** |
| CN117683763A (zh) * | 2022-09-09 | 2024-03-12 | 中国科学院遗传与发育生物学研究所 | 基于dna聚合酶的基因组编辑***和方法 |
| CN118086285B (zh) * | 2024-04-23 | 2024-07-19 | 天津凯莱英生物科技有限公司 | 蛋白定向进化的方法 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109517841A (zh) * | 2018-12-05 | 2019-03-26 | 华东师范大学 | 一种用于核苷酸序列修饰的组合物、方法与应用 |
| CN109957569A (zh) * | 2017-12-22 | 2019-07-02 | 中国科学院遗传与发育生物学研究所 | 基于cpf1蛋白的碱基编辑***和方法 |
| WO2019147014A1 (ko) * | 2018-01-23 | 2019-08-01 | 기초과학연구원 | 연장된 단일 가이드 rna 및 그 용도 |
| CN110835634A (zh) * | 2018-08-15 | 2020-02-25 | 华东师范大学 | 一种新型碱基转换编辑***及其应用 |
-
2020
- 2020-08-20 CN CN202080059623.XA patent/CN114945670A/zh active Pending
- 2020-08-20 WO PCT/CN2020/110207 patent/WO2021032155A1/zh active Application Filing
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109957569A (zh) * | 2017-12-22 | 2019-07-02 | 中国科学院遗传与发育生物学研究所 | 基于cpf1蛋白的碱基编辑***和方法 |
| WO2019147014A1 (ko) * | 2018-01-23 | 2019-08-01 | 기초과학연구원 | 연장된 단일 가이드 rna 및 그 용도 |
| CN110835634A (zh) * | 2018-08-15 | 2020-02-25 | 华东师范大学 | 一种新型碱基转换编辑***及其应用 |
| CN109517841A (zh) * | 2018-12-05 | 2019-03-26 | 华东师范大学 | 一种用于核苷酸序列修饰的组合物、方法与应用 |
Non-Patent Citations (2)
| Title |
|---|
| CHAO LI 等: "Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion", GENOME BIOLOGY, vol. 19, no. 1, pages 1, XP055655088, DOI: 10.1186/s13059-018-1443-z * |
| NICOLE M. GAUDELLI ET AL.: "Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage", NATURE, no. 551, 25 October 2017 (2017-10-25), pages 464 - 471 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115820603A (zh) * | 2022-11-15 | 2023-03-21 | 吉林大学 | 一种基于dCasRx-NSUN6单基因特异性M5C修饰编辑方法 |
| CN116751799A (zh) * | 2023-06-14 | 2023-09-15 | 江南大学 | 一种多位点双重碱基编辑器及其应用 |
| CN116751799B (zh) * | 2023-06-14 | 2024-01-26 | 江南大学 | 一种多位点双重碱基编辑器及其应用 |
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Effective date of registration: 20220922 Address after: Unit E598, 5th Floor, Lecheng Plaza, Phase II, Biomedical Industrial Park, No. 218, Sangtian Street, Suzhou Industrial Park, Suzhou Area, China (Jiangsu) Pilot Free Trade Zone, Suzhou City, Jiangsu Province, 215127 Applicant after: Suzhou Qihe Biotechnology Co.,Ltd. Address before: Room D340, F3, building 2, No. 2250, Pudong South Road, Pudong New Area, Shanghai 200120 Applicant before: Shanghai Blue Cross Medical Science Research Institute |