CN1149282C - Method for improving cellulase activity by using alcohol waste liquid of sugar refinery - Google Patents
Method for improving cellulase activity by using alcohol waste liquid of sugar refinery Download PDFInfo
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- CN1149282C CN1149282C CNB001143581A CN00114358A CN1149282C CN 1149282 C CN1149282 C CN 1149282C CN B001143581 A CNB001143581 A CN B001143581A CN 00114358 A CN00114358 A CN 00114358A CN 1149282 C CN1149282 C CN 1149282C
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- China
- Prior art keywords
- substratum
- cellulase
- waste liquid
- cellulase activity
- sugar refinery
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- 108010059892 Cellulase Proteins 0.000 title claims abstract description 30
- 229940106157 cellulase Drugs 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 17
- 230000000694 effects Effects 0.000 title claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims description 29
- 239000007788 liquid Substances 0.000 title abstract description 13
- 239000002699 waste material Substances 0.000 title abstract description 13
- 239000010902 straw Substances 0.000 claims description 13
- 235000015099 wheat brans Nutrition 0.000 claims description 9
- 241000609240 Ambelania acida Species 0.000 claims description 6
- 239000010905 bagasse Substances 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 4
- 240000008042 Zea mays Species 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
- 244000144725 Amygdalus communis Species 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 2
- 210000003608 fece Anatomy 0.000 claims 1
- 239000010871 livestock manure Substances 0.000 claims 1
- 238000000855 fermentation Methods 0.000 abstract description 13
- 230000004151 fermentation Effects 0.000 abstract description 13
- 239000000835 fiber Substances 0.000 abstract description 10
- 230000001954 sterilising effect Effects 0.000 abstract description 9
- 239000003337 fertilizer Substances 0.000 abstract description 5
- 238000002360 preparation method Methods 0.000 abstract description 4
- 230000001476 alcoholic effect Effects 0.000 abstract 2
- 241000233866 Fungi Species 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- 102000004190 Enzymes Human genes 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 6
- 239000000843 powder Substances 0.000 description 6
- 238000011160 research Methods 0.000 description 5
- 239000002361 compost Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 241000223259 Trichoderma Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000010808 liquid waste Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
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- 239000011573 trace mineral Substances 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 241000228212 Aspergillus Species 0.000 description 1
- 241001225321 Aspergillus fumigatus Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241001149951 Mucor mucedo Species 0.000 description 1
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 241001123663 Penicillium expansum Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000499912 Trichoderma reesei Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
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- 229910052799 carbon Inorganic materials 0.000 description 1
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- 238000011534 incubation Methods 0.000 description 1
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- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
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- Fertilizers (AREA)
Abstract
The invention is a method for improving cellulase activity, it adds the alcoholic waste liquid of sugar refinery into culture medium, through sterilizing, insert cellulase to produce fungus fermentation culture, the product obtained can be refined into cellulase preparation, can also add into fiber directly and ferment into feed and agricultural fertilizer, said method is simple, with low costs, can improve cellulase activity greatly, and has expanded the comprehensive utilization of alcoholic waste liquid, offer the new way for disposing the waste liquid of sugar refinery.
Description
Technical field
The present invention relates to utilize the waste material from sugar refinery alcohol slops to reach the method that improves cellulase activity as the cellulase producing bacteria substratum.
Background technology
Alcohol slops is the waste liquid that agricultural-food such as molasses or starch discharge behind fermentative production alcohol, and its organic concentration height, color are dark, and oxygen-consumption is big, and difficult treatment is serious source of pollution.But alcohol slops rich in proteins, amino acid, various saccharides and trace element, it also is the resource that a kind of utmost point has the value utilized, can therefrom extract amino acid, multiple useful composition such as glycerine, or as feed, fertilizer, pigment, raw materials such as building water reducer, center on the processing and the comprehensive utilization of alcohol waste liquid for sugar refinery at present, the scientific research personnel of environmental administration and grain distillery carried out the research of many aspects, many achievements have also been obtained, as Chinese patent CN92107109,95103709,96107697, the content that 96111883 grades are announced, disclose the several different methods that alcohol slops is handled, but the alcohol slops that just is limited to that these open source literatures are reported is preparing feed, the application of fertilizer.
Cellulase can decompose vegetable fibre, in modern science, it is widely used in feed, food, papermaking medicine, industries such as weaving, present research direction mainly is the aspects such as optimization at high vigor cellulase producing bacteria strain improvement and culture condition, the optimization of culture condition comprises suitable culture base and the suitable condition selected, we see from the document of open report, many scientific research persons have chosen many kinds of substratum, long agent such as nitrogenous source are urged in adding in substratum of having, carbon source, nutritive salt and inductor, and find out bacterial classification suitable culture concentration, temperature, then wait condition, live to improve cellulase amount and enzyme, but in the content of open source literature report, the incubation time that has is long, DeGrain, the complex process that has, cost is higher.
Summary of the invention
The purpose of this invention is to provide that a kind of cost is low, effective, method is simple, can improve the cellulase activating method by a relatively large margin.
Another object of the present invention is by the use to alcohol waste liquid for sugar refinery, finds the way of sugar refinery waste liquid comprehensive utilization, accomplishes to protect environment better, thoroughly administers sugar refinery waste liquid waste water.
The inventor finds when scientific research, in the cellulase producing bacteria culturing process, alcohol slops is added to substratum, can increase substantially cellulosic enzyme and live, and show by a series of experiments that it is cultivated with general ordinary method and compare, improve enzyme live 5-10 doubly more than.
The alcohol slops that adds 3-60BX, pH3.5-6.0 in the substratum that the present invention is achieved in that is abundant at raw material sources, lower-cost vegetable fibre or crop products are formed, add-on is the 10%-95% of substratum weight content, in 25-37 ℃ temperature, cultivate, the product that obtains can be refined into cellulase preparation, also it can be inoculated in the vegetable fibre, continue to enlarge fermentation culture, make animal and fowl fodder or be fermented into fertilizer.
Above-mentioned said cellulase produces substratum and comprises existing known Mandels substratum, or the substratum of straw and crop products combination, described plant straw comprises straw, bagasse, corn stalk, corn ear, Caulis arachidis hypogaeae, cotton stalk, and described crop products comprises wheat bran, cavings, soya-bean cake.
The kind of cellulase producing bacteria has a lot, the present invention selects for use healthy and free from worry wood mould (Trichoderrna koningii), Aspergillus fumigatus (Aspergillus fmigatus), Trichodermareesei (Trichoderma reesel), lignin wood mould (Trlchodermalignaram), viride (Trichoderma vivide), terreus (Asperglllus terreus), the strong mould (Penicillium funicilosum) of rope, Penicilllum expansum (Penicillium expansum), zhizopchin (Rhlzopuschinensis), head mold (Rhlzopus sp.), crinosity mould (Pestalotlopsls westerdl jki), mucor mucedo (Mucr mrcrdo) etc.
That the cultivation of cellulase producing bacteria can divide is solid-state, liquid state or semi-solid ferment, and culture temperature is a normal temperature, and the best is 25-37 ℃, and fermentation time is 24 hours to 7 days.When selecting plant straw and crop products to be substratum, add the high slightly alcohol slops of the optional concentration of alcohol slops, as 30BX-80BX, when adopting alcohol slops to make substratum, BX is 1-40.
The inventor has analyzed the composition of alcohol slops in experiment, find to contain a certain amount of carbohydrate, organic acid in the alcohol slops.The residual body of yeast, inorganic salt and trace element, also contain higher protein and amino acid, these compositions have had additional to the nutrition of substratum, make cellulase producing bacteria obtain sufficient nutrient during the fermentation, in addition, infer in the waste liquid waste liquid and may have some inducible factors or activator, in fermentation, promoted the generation of cellulase by synergy, and in the substratum of common Madels nutritive medium additive water, growth is denser although mycelium germination reaches spore soon, and enzyme is lived and the too late substratum height that adds alcohol slops.At present the inventor to its induce because of in or the activation reason further investigate, to find out a kind of method of simpler and easy and general raising cellulase activity.
The present invention compared with prior art, its substantive distinguishing features and progress are:
1. a kind of new cellulase-producing substratum is provided, can have improved cellulase activity effectively, cost is low.
2. can handle for alcohol slops and find a new way, accomplish utilization of waste material, reduce the environmental pollution that causes because of discharging.
3. also can provide outlet for the comprehensive utilization of agricultural crop straw, present straw great majority are made compost, because fiber indigestibility under usual conditions if add the plain enzyme of small amount of fibers, adds alcohol slops again, then can the adult shorten the compost fermentation time.
4. applicable to the producing of cellulase preparation, also can join in fermention medium or feed and the fertilizer, working method is simple, can wide popularization and application.
Embodiment
Embodiments of the invention are below described:
Embodiment one
Get sugar refinery bagasse and wheat bran, weight ratio 3: 2,100 kilograms, add 200 kilograms of 20Bx, the alcohol slops of pH4.3 mixes, and feeds steam sterilizing 20 minutes, and the plain enzyme of incoming fiber produces bacterium, cultivates 4 days in the time of 28 ℃, obtains solid koji.
Embodiment two
The percentage composition ratio is got bagasse 3% by weight, wheat bran 2%, and the alcohol slops 95% of the pH4.5 of 25BX, through sterilization, and the plain enzyme generation of incoming fiber bacterium, fermentation is 72 hours in the time of 30 ℃, can be used for producing cellulase preparation directly as additive for farm animal feed.
Embodiment three
Get straw powder 10%, Caulis arachidis hypogaeae powder 10%, the alcohol slops 60% of wheat bran 20% and 60BX, pH4.8 adds after 1 times of mixing of water 100 ℃ of sterilizations 5 minutes, and the plain enzyme of incoming fiber produces bacterium, and 37 ℃ of fermentation culture of temperature 84 hours can be used as the feed or the sort of quyi.
Embodiment four
Get bagasse 10%, cavings 10%, the alcohol slops 70% of soya-bean cake 10% and 50BX, pH5 adds 2 times in water, mixes the back sterilization, and the plain enzyme of incoming fiber produces bacterial classification, and fermentation culture is 84 hours in the time of 25 ℃, obtains semi-solid bent.
Embodiment five
Substratum consists of bagasse 20%, wheat bran 10%, and the alcohol slops 70% of 30BX, pH5.0 inserts bacterial classification through sterilization, and fermentation is 84 hours in the time of 28 ℃.
Embodiment six
Substratum consists of the alcohol slops 67% of straw powder 18%, wheat bran 15%, 2.5BX, pH5.5, and bacterial classification is inserted in the sterilization back, and fermentation is 96 hours in the time of 35 ℃.
Embodiment seven
Substratum consists of milled powders of cornstalk 15%, wheat bran or cavings 10%, the alcohol slops 75% of 20BX, pH6.0, and bacterial classification is inserted in the sterilization back, and fermentation is 84 hours in the time of 30 ℃.
Embodiment eight
Substratum consists of straw powder or Caulis arachidis hypogaeae powder 15%, wheat bran 13%, and the alcohol slops of 35BX, pH4.5 72% adds one times in water, and bacterial classification is inserted in the sterilization back, and fermentation is 96 hours in the time of 32 ℃.
Embodiment nine
Compare with Mandels substratum and the alcohol slops that adds the pH5.0 of 10%40BX, method is identical with embodiment five, and measurement result shows that cellulase activity can improve nearly 10 times in the fermented product of adding alcohol slops.
Embodiment ten
In straw or grass stalk compost, per 1000 kilograms of 10 kilograms of fermented liquids and 10 kg of water that add embodiment seven, compost rot effect than common not with the time shortening of fermented liquid 15 days.
Claims (3)
1, a kind of method that improves cellulase activity is characterized in that producing the alcohol slops that adds 3-60BX, pH3.5-6.0 in the substratum at cellulase, and add-on is the 10%-95% of substratum weight content, cultivates in 25-37 ℃ temperature.
2, according to the method for the described raising cellulase activity of claim 1, it is characterized in that cellulase produces substratum and comprises existing known Mandels substratum, or the substratum of plant straw and crop products combination, described plant straw comprises straw, bagasse, corn stalk, corn ear, Caulis arachidis hypogaeae, cotton stalk, and described crop products comprises wheat bran, cavings, soya-bean cake.
3, the method for raising cellulase activity according to claim 1 is characterized in that it is applied to make in feed and the fermented manure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001143581A CN1149282C (en) | 2000-01-26 | 2000-01-26 | Method for improving cellulase activity by using alcohol waste liquid of sugar refinery |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001143581A CN1149282C (en) | 2000-01-26 | 2000-01-26 | Method for improving cellulase activity by using alcohol waste liquid of sugar refinery |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1262327A CN1262327A (en) | 2000-08-09 |
| CN1149282C true CN1149282C (en) | 2004-05-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB001143581A Expired - Fee Related CN1149282C (en) | 2000-01-26 | 2000-01-26 | Method for improving cellulase activity by using alcohol waste liquid of sugar refinery |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102108347B (en) * | 2009-12-25 | 2012-10-31 | 中国科学院生态环境研究中心 | Method for increasing enzyme activity of cellulase produced from fungal |
| CN103820422B (en) * | 2014-02-26 | 2016-02-03 | 中南林业科技大学 | A kind of method improving mould cellulase-producing |
| CN105087657A (en) * | 2014-05-07 | 2015-11-25 | 李先强 | Method for brewing alcohol by using straw forage |
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- 2000-01-26 CN CNB001143581A patent/CN1149282C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1262327A (en) | 2000-08-09 |
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