CN114920357B - Method for relieving photo-inhibition of anaerobic ammonium oxidation sludge in short term - Google Patents

Method for relieving photo-inhibition of anaerobic ammonium oxidation sludge in short term Download PDF

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CN114920357B
CN114920357B CN202210697665.7A CN202210697665A CN114920357B CN 114920357 B CN114920357 B CN 114920357B CN 202210697665 A CN202210697665 A CN 202210697665A CN 114920357 B CN114920357 B CN 114920357B
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sludge
selenium
carbon quantum
doped carbon
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CN114920357A (en
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黄宝成
任之琪
张丽格
杜雪宁
金仁村
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Hangzhou Normal University
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/28—Anaerobic digestion processes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/30—Treatment of water, waste water, or sewage by irradiation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Abstract

The invention discloses a method for relieving photo-inhibition of anammox sludge in a short period, which comprises the following steps: the method comprises the steps of preparing selenium-doped carbon quantum dot solution by a hydrothermal carbonization method, adding the selenium-doped carbon quantum dot solution into anaerobic ammonium oxidation sludge matrix solution in different proportions, measuring the activity of the anaerobic ammonium oxidation sludge through batch experiments, measuring the contents of intracellular active oxygen and malonaldehyde, and finally quantitatively measuring and analyzing functional genes of the anaerobic ammonium oxidation sludge. According to the invention, the selenium-doped carbon quantum dots are utilized to remove active oxygen and absorb light with longer ultraviolet emission wavelength so as to resist the damage of ultraviolet light to bacteria, stable denitrification of the anaerobic ammonia oxidation reactor is realized under a high illumination condition, a solution is provided for the problem of photoinhibition of anaerobic ammonia oxidation sludge under the illumination condition, and the activity of the anaerobic ammonia oxidation sludge can be recovered to a dark level after the selenium-doped carbon quantum dots are added. Has the advantages of simple operation, low cost and the like, and provides a new method and thought for the stable operation of the anaerobic ammonia oxidation process.

Description

Method for relieving photo-inhibition of anaerobic ammonium oxidation sludge in short term
Technical Field
The invention relates to the technical field of water treatment, in particular to a method for relieving photo-inhibition of anaerobic ammonium oxidation sludge in a short period.
Background
Anammox is a biological reaction that produces nitrogen and nitrate under anoxic conditions with ammonia as an electron donor and nitrite as an electron acceptor. In recent years, the anaerobic ammonium oxidation autotrophic nitrogen removal process is gradually emphasized and widely applied because the anaerobic ammonium oxidation reaction does not need an additional carbon source or aeration. However, the long growth cycle of anammox bacteria for 10-12 days and their sensitivity to environmental conditions have hindered the development of this technology in practical applications.
Since the anaerobic ammonia oxidizing bacteria grow slowly, the change of environmental factors such as illumination, temperature, pH, dissolved oxygen and the like can obviously influence the sludge activity, thereby causing the deterioration of the performance of the reactor. In the anammox process, the light conditions are an important control parameter, and since anammox bacteria are photosensitive microorganisms, the growth activity of the anammox bacteria is inhibited by the light. Therefore, in the actual operation of the reactor, shading measures are usually adopted to avoid the negative influence of light on the anaerobic ammonia oxidation sludge.
High intensity light can generate active oxygen in living cells, and if the generation rate of the active oxygen exceeds the degradation rate of the active oxygen, the excessive active oxygen can attack biomacromolecules such as proteins, nucleic acids and lipids, thereby causing oxidative damage. In order to maintain redox balance, microorganisms form a complete set of protection mechanisms. The antioxidant system consists of non-enzymatic systems (such as ascorbic acid and flavonoids) and enzymatic systems (such as glutathione peroxidase, superoxide dismutase, catalase).
Patent specification with publication number CN108504619B discloses a method for relieving photo-inhibition of haematococcus pluvialis, wherein when the haematococcus pluvialis cell culture reaches a plateau stage, the illumination intensity is changed to strong light. The photoinhibition of algae cells is relieved by adding glycerol or sodium acetate to the culture medium. However, small molecular organic substances need to be added once or multiple times during the switching of the culture stage, the steps are complicated, and the requirements on operating conditions are high.
The prior art does not solve the problem of how to relieve the photoinhibition of photosensitive anammox bacteria in the aspect of oxidative stress of the anammox reactor under the illumination condition. When the excess of active oxygen is generated due to the change of external conditions such as light, it is necessary to add an external additive such as an antioxidant or an active oxygen scavenger to the system to relieve the oxidative stress to which the anammox sludge is subjected. Therefore, the effect of adding the selenium-doped carbon quantum dots to relieve the light inhibition of the active oxygen of the anaerobic ammonia oxidizing bacteria under the illumination condition has important significance.
Disclosure of Invention
The invention aims to provide a method for relieving the photoinhibition of anammox sludge in a short time aiming at the defects of anammox reaction. The method has the advantages that the selenium-doped carbon quantum dots are utilized to eliminate active oxygen and absorb ultraviolet light and emit light with longer wavelength to resist the damage of the ultraviolet light to bacteria, a solution is provided for the problem of photoinhibition of the anaerobic ammonia oxidation sludge under the illumination condition, and the activity of the anaerobic ammonia oxidation sludge can be recovered to the dark level after the selenium-doped carbon quantum dots are added. The method is simple and convenient to operate, has low cost, can improve the activity of the anaerobic ammonium oxidation sludge reactor under illumination, and is favorable for stable operation of the anaerobic ammonium oxidation reactor.
A method for relieving the photoinhibition of anaerobic ammonium oxidation sludge in a short period comprises the following processing steps:
(1) Preparing selenium-doped carbon quantum dots: the method comprises the steps of preparing selenium-doped carbon quantum dots by taking selenocysteine as a synthetic raw material through a hydrothermal carbonization method, dissolving the selenium-doped carbon quantum dots in ultrapure water, adjusting the pH to be alkaline to promote the dissolution of the selenocysteine, transferring the solution to a reaction kettle to react at 55-65 ℃, and centrifuging after the reaction is finished to obtain a supernatant, namely the selenium-doped carbon quantum dot solution.
(2) And adding the prepared selenium-doped carbon quantum dots into an anaerobic ammonia oxidation sludge system cleaned by an inorganic salt solution, and maintaining the activity of the anaerobic ammonia oxidation sludge under the illumination condition so as to realize the stable operation of the reactor.
Preferably, in the step (1), the selenocysteine solution prepared by using ultrapure water has a mass content of 1.5-2.0%.
Preferably, in step (1), in order to completely dissolve selenocysteine, the pH value of the solution in step (1) is adjusted to 10-11 by using a sodium hydroxide solution.
Preferably, the reaction condition of the hydrothermal carbonization method is that the reaction is carried out for 23 to 25 hours at the temperature of 58 to 62 ℃.
Preferably, the centrifugation parameters of step (1): rotating at 13000-14000 Xg, and centrifuging for 14-16 minutes.
Preferably, in the step (2), the anaerobic ammonia oxidation sludge reaction system is 100ml, wherein the sludge amount is 1.5-2.5g/L VSS.
Preferably, the anaerobic ammonia oxidation sludge system in the step (2) is formed by mixing anaerobic ammonia oxidation granular sludge and a matrix solution, wherein the matrix solution consists of 90-110mg/L of ammonia nitrogen, 90-110mg/L of nitrite nitrogen, an inorganic salt solution and trace elements, and the pH value of the matrix solution is adjusted to 7.45-7.55 by using a hydrochloric acid solution.
Preferably, the amount of the selenium-doped carbon quantum dots used in step (2) is 0.25-0.5mL/g VSS, more preferably 0.42mL/g VSS.
Preferably, the inorganic salt solution in step (2) is prepared from NaH 2 PO 4 、CaCl 2 ·2H 2 O、MgSO 4 ·7H 2 O and NaHCO 3 And (4) forming.
Preferably, the trace elements in step (2) are EDTA and FeSO 4 ·7H 2 O、H 3 BO 4 、MnCl 2 ·4H 2 O、CuSO 4 ·5H 2 O、ZnSO 4 ·7H 2 O、NiCl 2 ·6H 2 O、NaMoO 4 ·2H 2 O and CoCl 2 ·6H 2 And (C) O.
Preferably, in step (2), in order to avoid the inhibition of the production of the anammox bacteria by the dissolved oxygen, nitrogen or argon is aerated for at least 10 minutes.
Preferably, the light intensity in step (2) is 50,000Lux.
Preferably, the water bath reaction temperature in the step (2) is 34-36 ℃, and the reaction is carried out for 4 hours.
And determining the anaerobic ammonia oxidation activity of the sludge according to the matrix consumption rate and the concentration of the volatilizable suspended solid in the sludge in the experiment.
Preferably, the dominant bacteria in the anammox sludge in step (2) is Candidatus Kuenenia.
The anaerobic ammonia oxidation sludge activity in the selenium-doped carbon quantum system can be recovered to a dark level. In the step (2), the activity of the anaerobic ammonia oxidation sludge is reduced to 71.9 +/-5.2% under the condition of dark only under the condition of light. When 0.42mL/g VSS of selenium-doped carbon quantum dots are added in the step (2), the activity of the anaerobic ammonium oxidation sludge is recovered to 102.9 +/-4.8% under the dark condition.
Under the method, the activity of the anaerobic ammonia oxidation sludge under the illumination condition is improved in a short time. The application of the selenium-doped carbon quantum dots in an anaerobic ammonia oxidation sludge system can generate excessive active oxygen in anaerobic ammonia oxidation sludge under the illumination condition, thereby causing damage to bacteria. The selenium-doped carbon quantum dots can be combined with microorganisms to emit light with longer wavelength after absorbing ultraviolet light, so that the damage of the unfavorable ultraviolet light to bacteria is prevented. Meanwhile, the selenium-doped carbon quantum dots have the capacity of removing active oxygen hydroxyl radicals, and can help the anaerobic ammonium oxidation sludge to resist the damage of oxidative stress, so that the activity of the anaerobic ammonium oxidation sludge is maintained, and the stable denitrification effect of the anaerobic ammonium oxidation reactor under the illumination condition is realized.
Compared with the prior art, the invention has the main advantages that:
1. the problem of photoinhibition of the anaerobic ammonium oxidation sludge is solved, the inhibitory effect of an illumination system on anaerobic ammonium oxidation bacteria is effectively relieved, and the activity of the anaerobic ammonium oxidation sludge under illumination conditions reaches the dark level.
2. The selenium-doped carbon quantum dots have the advantages of good biocompatibility, low cost, low biological toxicity and the like, and are suitable for solving the problem of photoinhibition of the anaerobic ammonium oxidation sludge.
Drawings
FIG. 1 is a diagram showing the result of activity measurement of anammox sludge in a reaction system in which selenium-doped carbon quantum dots with different concentrations are added;
FIG. 2 is a graph showing the results of measuring the activity of anammox sludge under different wave bands of illumination;
FIG. 3 is a graph showing the results of measuring the intracellular active oxygen and malonaldehyde content of sludge in a reaction system in which selenium-doped carbon quantum dots with different concentrations are added;
FIG. 4 is a diagram showing the quantitative determination result of the functional genes of the anaerobic ammonia oxidation sludge in a reaction system in which selenium-doped carbon quantum dots with different concentrations are added.
Detailed Description
As described above, in view of the deficiencies of the prior art, the present inventors have made extensive studies and extensive practices, and propose a technical solution of the present invention, which is mainly based on at least: the invention provides application of selenium-doped carbon quantum dots in an anaerobic ammonia oxidation sludge system, wherein the anaerobic ammonia oxidation sludge can generate excessive active oxygen under the illumination condition, so that bacteria are damaged. The selenium-doped carbon quantum dots can be combined with microorganisms to emit light with longer wavelength after absorbing ultraviolet light, so that the damage of the unfavorable ultraviolet light to bacteria is prevented. Meanwhile, the selenium-doped carbon quantum dots have the capacity of removing active oxygen hydroxyl radicals, and can help the anaerobic ammonium oxidation sludge to resist the damage of oxidative stress, so that the activity of the anaerobic ammonium oxidation sludge is maintained, and the stable denitrification effect of the anaerobic ammonium oxidation reactor under the illumination condition is realized. The problem of photoinhibition of the anaerobic ammonium oxidation sludge is solved, the inhibitory effect of an illumination system on anaerobic ammonium oxidation bacteria is effectively relieved, and the activity of the anaerobic ammonium oxidation sludge under illumination conditions reaches the dark level.
The invention provides a method for relieving photo-inhibition of anammox sludge in a short period, which comprises the following treatment steps:
(1) Preparing selenium-doped carbon quantum dots: the method comprises the steps of preparing selenium-doped carbon quantum dots by taking selenocysteine as a synthetic raw material through a hydrothermal carbonization method, dissolving the selenium-doped carbon quantum dots in ultrapure water, adjusting the pH to be alkaline to promote the dissolution of the selenocysteine, transferring the solution to a reaction kettle to react at 55-65 ℃, and centrifuging after the reaction is finished to obtain a supernatant, namely the selenium-doped carbon quantum dot solution.
(2) And adding the prepared selenium-doped carbon quantum dots into an anaerobic ammonia oxidation sludge system cleaned by an inorganic salt solution, and maintaining the activity of the anaerobic ammonia oxidation sludge under the illumination condition so as to realize the stable operation of the reactor.
Preferably, in the step (1), the selenocysteine solution prepared by ultrapure water has a mass content of 1.5-2.0%.
Preferably, in step (1), in order to completely dissolve selenocysteine, the pH value of the solution in step (1) is adjusted to 10-11 by using a sodium hydroxide solution.
Preferably, the hydrothermal carbonization reaction is carried out for 23-25h under the environment of 58-62 ℃.
Preferably, the centrifugation parameters of step (1): rotating at 13000-14000 Xg, and centrifuging for 14-16 minutes.
Preferably, in the step (2), the anaerobic ammonia oxidation sludge reaction system is 100ml, wherein the sludge amount is 1.5-2.5g/L VSS.
Preferably, the anaerobic ammonia oxidation sludge system in the step (2) is formed by mixing anaerobic ammonia oxidation granular sludge and a matrix solution, wherein the matrix solution consists of 90-110mg/L of ammonia nitrogen, 90-110mg/L of nitrite nitrogen, an inorganic salt solution and trace elements, and the pH value of the matrix solution is adjusted to 7.45-7.55 by using a hydrochloric acid solution.
Preferably, the amount of the selenium-doped carbon quantum dots used in step (2) is 0.25-0.5mL/g VSS, more preferably 0.42mL/g VSS.
Preferably, the inorganic salt solution in step (2) is prepared from NaH 2 PO 4 、CaCl 2 ·2H 2 O、MgSO 4 ·7H 2 O and NaHCO 3 And (4) forming.
Preferably, the trace elements in step (2) are EDTA and FeSO 4 ·7H 2 O、H 3 BO 4 、MnCl 2 ·4H 2 O、CuSO 4 ·5H 2 O、ZnSO 4 ·7H 2 O、NiCl 2 ·6H 2 O、NaMoO 4 ·2H 2 O and CoCl 2 ·6H 2 And (C) O.
Preferably, in step (2), in order to avoid the inhibition of the production of the anammox bacteria by the dissolved oxygen, nitrogen or argon is aerated for at least 10 minutes.
Preferably, the light intensity in step (2) is 50,000Lux.
Preferably, the water bath reaction temperature in the step (2) is 34-36 ℃, and the reaction is carried out for 4 hours.
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. In addition, the technical features involved in the respective embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The invention is further described with reference to the following drawings and specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The following examples are conducted under conditions not specified, usually according to conventional conditions, or according to conditions recommended by the manufacturer.
Example 1
(1) The selenium-doped carbon quantum dots are prepared by a hydrothermal carbonization method, a 15% selenocysteine solution is prepared by ultrapure water, and the pH of the solution is adjusted to 10 by a sodium hydroxide solution. And transferring the solution into a reaction kettle, reacting for 24 hours at the temperature of 60 ℃, centrifuging for 15 minutes at the rotating speed of 13682 Xg after the reaction is finished, and obtaining supernate, namely the required selenium-doped carbon quantum dot solution.
(2) A certain amount of anaerobic ammonium oxidation sludge is taken, washed with an inorganic salt solution for three times, and then washed with pure water for three times. The inorganic salt solution is prepared from NaH 2 PO 4 、CaCl 2 ·2H 2 O、MgSO 4 ·7H 2 O and NaHCO 3 And (4) forming.
(3) Anaerobic ammonia oxidation granular sludge is taken to be put into 100mL of matrix liquid, and the matrix liquid consists of 100mg/L of ammonia nitrogen, 100mg/L of nitrite nitrogen, inorganic salt solution and trace elements. Wherein the ammonia nitrogen and the nitrite nitrogen consist of (NH) 4 ) 2 SO 4 With NaNO 2 Preparing the stock solution. The trace elements are EDTA and FeSO 4 ·7H 2 O、H 3 BO 4 、MnCl 2 ·4H 2 O、CuSO 4 ·5H 2 O、ZnSO 4 ·7H 2 O、NiCl 2 ·6H 2 O、NaMoO 4 ·2H 2 O and CoCl 2 ·6H 2 And (C) O.
(4) Respectively adding 0.42mL/g selenium-doped carbon quantum dot solution -1 VSS in the matrix solution to perform batch test to determine the activity of the anaerobic ammonia oxidation sludge.
(5) The batch experiment was carried out in a quartz water bath circulating flask with a temperature controlled at 35 + -1 deg.C and the illumination conditions set to 50,000Lux illumination intensity simulated by a xenon lamp. Meanwhile, the sludge activity of different wave bands is measured (selenium-doped carbon quantum dots are added under the conditions of >420nm, <420nm and <420 nm). And (3) carrying out experiment co-culture for 4 hours, taking 2mL of supernatant every 1 hour to determine the concentration of ammonia nitrogen and nitrite nitrogen in the sample, and determining the concentration of the volatilizable suspended solid in the anaerobic ammonia oxidation sludge after the experiment is finished.
(6) After the reaction is finished, active oxygen and malondialdehyde in the sludge sample measuring bag are measured, and the anaerobic ammonium oxidation sludge functional genes hzsA, hdh and nirS are quantitatively measured.
Comparative example 1
In comparison with example 1, comparative example 1 was performed for 4 hours under dark conditions for the reaction system of the anammox sludge. As shown in FIG. 1, the results of the activity measurement of anammox sludge showed that 0.42mL g was added in example 1 -1 VSS selenium-doped carbon quantum dot solution enables the activity of the anaerobic ammonia oxidation sludge to reach 102.9 +/-4.8% in the dark.
Comparative example 2
In comparison with example 1, comparative example 1 was conducted under a xenon lamp simulated sunlight 50,000 illumination for 4 hours for the reaction system of the anammox sludge. The results of the anammox sludge activity measurement show that the light conditions allow the anammox sludge activity to reach 71.9 ± 5.2% of that in the dark, as shown in fig. 1. The anammox sludge activity shown in FIG. 2 indicates that the photoinhibition of the sludge is mainly due to UV light, in<Under the illumination condition of 420nm, the sludge activity can reach 342.1 +/-0.3 mg N.g by adding the selenium-doped carbon quantum dots -1 VSS·d -1 。
The measurement results of active oxygen and malondialdehyde are shown in figure 3, the active oxygen level in the sludge cells under the illumination condition is 234.8 +/-4.1% under the dark condition compared with the active oxygen in the sludge cells under the dark condition, but the addition of the selenium-doped carbon quantum dots can obviously reduce the active oxygen level in the cells, and the addition of 0.42mL/g -1 The active oxygen in the VSS selenium-doped carbon quantum dot system cell is only 127 +/-4.7% under the dark condition. The excessive reactive oxygen species in the bacteria cells trigger oxidative stress that results in bacterial damage. Upper level of malondialdehyde in lighting systemThe conclusion is proved by the rise that the content of the malondialdehyde in the sludge is increased to 112.7 +/-18.8 percent under the dark condition under the high illumination condition, and 0.42mL/g is added -1 After the VSS selenium-doped carbon quantum dots, the content of malondialdehyde is reduced by 28.4 +/-2.7 percent compared with the illumination condition.
The quantitative results of the anammox sludge functional genes hzsA, hdh and nirS are shown in FIG. 4. The absolute abundance of hzsA in light conditions was from 4.64X 10 compared to dark conditions 7 ±2.77×10 6 Down to 2.93X 10 7 ±5.10×10 5 . The absolute abundance of hdh compared to dark conditions is from 1.07X 10 7 ±2.81×10 5 Down to 5.8X 10 6 ±3.75×10 5 . nirS is from 1.65X 10 7 ±8.17×10 5 Down to 9.37X 10 6 ±5.20×10 5 . For the addition of 0.42 mL-g -1 For the VSS selenium-doped carbon quantum dot system, the absolute abundances of hzsA, hdh and nirS can reach 4.21 × 10 7 ±7.08×10 5 ，9.04×10 6 ±5.86×10 5 And 1.73X 10 7 ±4.10×10 4 。
Example 2
The difference from example 1 is that the concentration of the added selenium-doped carbon quantum dots is 0.25 mL-g -1 VSS, the rest conditions are the same.
Example 3
The difference from example 1 is that the concentration of the added selenium-doped carbon quantum dots is 0.32 mL-g -1 VSS, the rest conditions are the same.
Example 4
The difference from example 1 is that the concentration of the added selenium-doped carbon quantum dots is 0.38 mL-g -1 VSS, the rest conditions are the same.
Example 5
The difference from example 1 is that the concentration of the added selenium-doped carbon quantum dots is 0.5 mL-g -1 VSS, the rest conditions are the same.

Claims (10)

1. A method for relieving the photoinhibition of anaerobic ammonium oxidation sludge in a short time is characterized by comprising the following processing steps:
(1) Preparing the selenium-doped carbon quantum dots:
dissolving selenocysteine in ultrapure water, adjusting the pH to be alkaline, and promoting the dissolution of the selenocysteine to obtain a selenocysteine solution; transferring the selenocysteine solution into a reaction kettle for reaction at the temperature of 55-65 ℃, and centrifuging after the reaction is finished to obtain supernatant, namely selenium-doped carbon quantum dot solution;
(2) And adding the prepared selenium-doped carbon quantum dots into an anaerobic ammonia oxidation sludge system cleaned by an inorganic salt solution, and maintaining the activity of the anaerobic ammonia oxidation sludge under the illumination condition so as to realize the stable operation of the reactor.
2. The method for relieving the photo-inhibition of the anammox sludge in a short term according to claim 1, wherein in the step (1), the selenocysteine solution has a mass content of 1.5-2.0% and a pH value of 10-11.
3. The method for alleviating the photo-inhibition of the anammox sludge in a short period of time according to claim 1, wherein in the step (1), the reaction condition of the reaction kettle is 58-62 ℃ for 23-25h.
4. The method for alleviating the photoinhibition of the anammox sludge in a short period of time according to claim 1, wherein in the step (2), the reaction system of the anammox sludge is 100ml, and the amount of the sludge is 1.5-2.5g/L VSS.
5. The method for relieving the photo-inhibition of the anaerobic ammonia oxidation sludge in a short term according to claim 1, wherein the anaerobic ammonia oxidation sludge system in the step (2) is formed by mixing anaerobic ammonia oxidation granular sludge and a matrix solution, wherein the matrix solution consists of 90-110mg/L of ammonia nitrogen, 90-110mg/L of nitrite nitrogen, an inorganic salt solution and trace elements, and the pH value is 7.45-7.55.
6. The method for alleviating photo-suppression of anammox sludge in a short term as claimed in claim 1, wherein the amount of selenium-doped carbon quantum dots used in step (2) is 0.25-0.5mL/g VSS.
7. The method of claim 1, wherein nitrogen or argon is sparged for at least 10 minutes prior to step (2) to avoid inhibition of anammox bacteria production by dissolved oxygen.
8. The method for alleviating the photo-inhibition of the anammox sludge in a short period of time according to claim 1, wherein the irradiation of the xenon lamp simulated sunlight in the step (2) is performed at an intensity of 50,000Lux.
9. The method for alleviating the photoinhibition of the anaerobic ammonium oxidation sludge in a short time according to claim 1, wherein the water bath reaction temperature in the step (2) is 34-36 ℃ and the reaction is carried out for 4 hours.
10. The method for alleviating photo-suppression of anammox sludge in a short period of time according to claim 1, wherein the dominant bacteria in the anammox sludge in step (2) is Candidatus Kuenenia.
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