CN114916631A

CN114916631A - Ultrahigh-purity octacosanol-flavone composite deuterium-depleted water combined and co-refined high-energy beverage and preparation method thereof

Info

Publication number: CN114916631A
Application number: CN202210486849.9A
Authority: CN
Inventors: 谭卓华; 黄炜岚; 谭晓露
Original assignee: Guangzhou Lvhui New Material Research Institute Co ltd
Current assignee: Guangzhou Lvhui New Material Research Institute Co ltd
Priority date: 2022-05-06
Filing date: 2022-05-06
Publication date: 2022-08-19
Also published as: WO2023213325A1

Abstract

The invention relates to a high-energy beverage synergistically refined by ultra-high-purity octacosanol-flavone composite deuterium-removed water and a preparation method thereof, wherein the beverage comprises the following components: octacosanol, flavone, deuterium-removed water, a stabilizer, a compound emulsifier, water-soluble dietary fiber, a compound electrolyte, a preservative and an acidulant. According to the characteristics of the extracts, the extracts are crossed and integrated, the ultra-high purity extract is introduced into the beverage, the concept of passive supplementation is changed, the making concept of active conditioning beverage is created, the effects of complementation and synergy of the beverage on organism regulation are reasonably and effectively and greatly enhanced and improved, the beverage has more superior effects on the effects of fatigue resistance, health care for improving the functions of human bodies, beauty treatment, weight reduction and the like, and the development of the fields of disease prevention and the like is further expanded; according to actual needs, the material is prepared from halal authentication raw materials without religious distinction by adjusting the change of components and being used in the fields of health care, new generation sports drinks, astronauts, military development and military high-energy drinks and the like.

Description

Ultrahigh-purity octacosanol-flavone composite deuterium-depleted water combined and co-refined high-energy beverage and preparation method thereof

Technical Field

The invention relates to the technical field of food processing, and particularly designs a high-energy beverage cooperatively refined by combining ultrahigh-purity octacosanol-flavone composite deuterium-depleted water and a preparation method thereof.

Background

The octacosanol as one kind of natural high-grade fatty alcohol has excellent biodegradability and easy absorption by human body

In table i, since 1949, it took 20 years for doctor t.k.cureton, university of elilinois, and the like, and 894 test persons were subjected to 42 test studies, and the persons who participated in the experiment had swim, wrestling, track and field teams, students in sports discipline, navy divers, and the like. Summarizing the results of these experiments, octacosanol has a metabolic rate and a decreased muscle friction, thereby achieving the effects of decreasing the necessary oxygen demand, improving the reaction time, eliminating muscle pain, etc., and these effects are interrelated; the main effects of the octacosanol on promoting the physiological functions of the human body are determined as follows:

enhancing endurance, energy and physical strength;

improving muscle strength (muscle function); eliminating muscle pain and reducing muscle friction;

shortening the muscle nerve reaction time and improving the stress ability; the reaction sensitivity is improved;

fourthly, strengthening the heart function and improving the cardiac muscle function;

improving stress ability and metabolism rate;

sixth, the resistance to stress of high mountain is enhanced;

seventhly, reducing the systolic blood pressure;

improving oxygen transportation energy and organism oxygen utilization rate and reducing necessary oxygen demand;

ninthly, stimulating hormone;

reducing cholesterol in the red;

the disease with higher morbidity which threatens the health of modern human beings is prepared by the following steps

As early as 2006, Chong Tou et al have demonstrated that octacosanol has a hypolipidemic effect. The qidao et al propose that the hypolipidemic effect of octacosanol is related to dosage, and the higher dosage of the substance can obviously reduce the content of TG, TC and LDL-C in serum; whereas low doses only have an effect on the concentration of TC in serum. Singh et al, that octacosanol reduces serum cholesterol levels by phosphorylating AMP-kinase to activate enzymatic activity, thereby reducing HMG-CoA enzymatic activity and achieving the goal of controlling serum cholesterol synthesis;

the octacosanol can reduce excessive calcium and fat content in blood, and is a calcitonin formation promoter

Can be used for treating hypercalcemia, osteoporosis, hypercholesterolemia, and hyperlipoproteinemia, and can be used for treating early-stage Parkinson disease of the elderly. Proved by many parties, octacosanol is known as a compound capable of relieving physical fatigue and having good biological activity. Research shows that the substance can improve the endurance of human body, recover physical strength rapidly, and improve kinetic energy such as related physiological indexes in body fluid after activity;

fourth, the blood sugar level and the serum testosterone level of the rats with the sports fatigue can be improved, the myocardial injury can be prevented, and the reproductive disorder of human bodies can be prevented; the anti-hypoxia anti-tumor pharmaceutical composition can improve the anti-hypoxia capability of an organism, has a treatment effect on diseases, and researches show that octacosanol can relieve slight dilatation and cellular edema of blood vessels of heart and brain tissues, has a certain protection effect on the structures of tissue cells, and also has potential anti-inflammatory and anti-tumor effects;

the octacosanol has the remarkable characteristics of extremely small dosage and remarkable physiological activity, can quickly eliminate fatigue and enhance physical strength and endurance capacity, and is a worldwide recognized anti-fatigue functional substance. According to the report of the Nippon grease Co., Ltd, the goal of eliminating fatigue can be achieved by orally taking 0.2mg of octacosanol per person per day; for physical and endurance enhancement, the daily oral dose is about 0.5 mg; and 5mg for therapeutic purposes;

sixthly, the security of the sixteenth party is extremely high. Proved by oral test of mice, the LD of the octacosanol ₅₀ 18000 mg/kg; safety ratio of salt (LD) ₅₀ =3000mg/kg) is much higher; clinical practice proves that the medicine can be taken for a long time without side effect.

The capsule wall component bamboo leaf flavone belongs to one of plant flavone, and its effective components are flavone glycoside and coumarin lactone. The components of the bamboo leaf flavone, besides flavonoids, also include phenolic acid, anthraquinone compounds, aromatic components and microelements such as manganese, zinc and tin, which together form the basis of the wide physiological and pharmacological activities of the bamboo leaf flavone

First, a study conducted by the dutch expert finds: experiments with 4807 participants showed that people drinking 375 ml of green tea daily had half the probability of heart disease as those who did not drink tea; the incidence of fatal heart disease is only one third. The important reasons are the flavones contained in green tea (< us clinical nutrition > > 2002.4.25);

the research on the physiological efficacy of the bamboo leaf flavone shows that:

the Chinese medicinal composition has the effects of removing oxygen free radicals, resisting aging, preventing senile dementia, preventing angiosclerosis, resisting vascular proliferation, resisting myocardial ischemia, lowering blood pressure, reducing blood fat and protecting heart and cerebral vessels, and is used for preventing and treating cardiovascular and cerebrovascular diseases, such as reducing fragility of blood vessels, relaxing blood vessels, improving permeability of blood vessels, preventing arteriosclerosis, reducing blood fat and cholesterol, preventing and treating senile hypertension, cerebral hemorrhage, coronary heart disease, angina pectoris, dilating coronary vessels, increasing coronary flow, reducing blood sugar and the like;

apoptosis of cancer cells and tumor cells can be induced, the anti-cancer and anti-tumor effects are exerted, and the apoptosis of normal tissue cells is delayed;

pharmacological effects of radiation protection, liver toxicity relief, acute and chronic hepatitis and liver cirrhosis treatment, organism immunity improvement, purgation, cough relief, phlegm elimination, asthma relief, spasmolysis, allergy resistance, virus resistance and the like;

almost all flavonoids have varying degrees of bacteriostatic activity against many microorganisms (including gram-positive, gram-negative and fungi);

fifthly, the immunity function of the organism can be improved, and the health of the organism is promoted;

sixthly, the medicine has more medicinal effects and better stability, and the structure contains phenolic hydroxyl which is consistent with the acid environment of gastric juice and intestinal juice of a human body, so that the first pass effect can be avoided;

has the following outstanding advantages:

the structure is stable and is not easy to degrade;

secondly, the medicine can go deep into the focus part to directly exert curative effect;

the hydrophilicity is enhanced, which is beneficial to the development of medicines, foods, cosmetics and the like;

good effect of anti-fatigue

Oxidative stress has been shown to cause chronic fatigue by increasing the release of proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and IL-1 beta;

many studies have shown that flavonoid supplementation can reduce muscle fatigue in fatigued rats. In the research of flavonoids compounds, lutein-6-C-neohesperidin luteolin-6-C-neohesperidoside (LN) separated from moso bamboo has an anti-fatigue effect on mice in a forced swimming experiment of a mouse load test. 3-week rat swimming model verification proves that LN can obviously prolong the fatigue time of rats, thereby realizing the anti-fatigue effect;

anti-aging effect of the three

The human diseases are more than 70, including cancer and cardiovascular and cerebrovascular diseases, which are related to free radicals, and the bamboo leaf flavone can effectively remove the free radicals to generate the function of antioxidation, thereby achieving the purpose of delaying senility;

efficacy of weight reduction

The excessive nutrition of a human body is converted into fat, and the fat stored in the body is oxidized and denatured, which is one of the reasons that fat people are difficult to lose weight;

fifthly, is a natural anti-oxide

The content of blood lactic acid after exercise can be obviously reduced, and the activity of lactate dehydrogenase is enhanced;

secondly, the antioxidant performance can be compared favorably with VE, and as a novel antioxidant, the bamboo leaf flavone can prevent or delay food from being oxidized, improve the stability of the food and prolong the storage period;

sixthly, the food has an antibacterial function and can be applied to the food industry;

safe and nontoxic

In the active ingredients, no toxic and harmful ingredients are found so far, and the active ingredients are not harmful to human bodies unlike ginkgo leaves which contain a certain amount of ginkgolic acid;

the efficacy of bamboo leaves is documented in ancient books such as Bencao Zhenzhen and Yaohai Yi and in the traditional Chinese medicine dictionary;

in 1998, (unsalted) bamboo leaves are approved by the Ministry of health to be listed in the list of natural plants for both medicine and food. "the visible bamboo leaves themselves have safe usability;

recent research reports show that: the bamboo leaf flavone is subjected to an acute toxicity test (a Hohner method), and the tested bamboo leaf flavone has no mutation effect according to an Ames test result judgment standard;

and the Zhejiang medical academy of sciences in 1998 + 1999 performs toxicological safety evaluation on the bamboo leaf flavonoid powder and the bamboo leaf flavonoid, and proves that the bamboo leaf flavonoid has no toxic or side effect and high safety.

⒊ present situation, direction and emphasis of development

Preparation method of octacosanol

At present, the main extraction raw materials of octacosanol are cane, bran wax, sago, wheat germ oil, and the octacosanol is insoluble in water, soluble in an organic solvent, and the existing substances have complex structures, so how to extract natural substances efficiently, economically and environmentally, a large amount of high-quality substances are obtained and the problems to be solved in production are solved, at present, the raw materials are saponified and then extracted by using an organic solvent (normal hexane, acetone and the like) to obtain a white or pale yellow powdery product, the powdery crystal is a natural high-grade alkanol mixture (containing 24-34 carbon original high-grade alkanol), the octacosane content in the mixture is 50-70%, and related pharmacological experiments in data are also carried out by using the extract with the octacosanol content. The prior product technology is only that the conventional extraction purity of octacosanol can only reach 40-80 percent, such as: a sports beverage and its manufacturing method-200510036559.0 (unauthorized-unpaid annual fee) according to claim 5, wherein: the purity of the octacosanol used is more than 80%. Such as: wheat germ beverage and its preparation method-201010615889.6 (No-no-year-paid) right

The method comprises the following steps of (1) extracting octacosanol from wheat germs by a supercritical CO2 extraction method to obtain white flaky octacosanol crystals, wherein the content of octacosanol is 42.1-43.5%;

extracting method of traditional flavone

The solubility characteristics of flavonoids vary greatly due to their different structures and sources, and thus, suitable solvents should be selected for extraction according to their polarity and water solubility. With the continuous and intensive research, bamboo leaf flavone is increasingly extracted, and according to the literature, the common extraction methods of bamboo flavone compounds include a hot water extraction method, an organic solvent extraction method, a microwave extraction method, an ultrasonic extraction method, a supercritical fluid extraction method, an enzyme extraction method, a crushing extraction method and other methods;

the experimental result shows that the efficiency of microwave extraction of bamboo leaf flavone is highest, the efficiency of ultrasonic extraction is second, the water extraction efficiency is worst, the time consumption is long, and the extraction rate is low, but the stability of the bamboo leaf flavone obtained by water extraction is relatively good;

the extracting solution obtained by the single method has good stability in the pH range of 6-7, and the extracting solution is not beneficial to storage and use under the conditions of higher temperature than 50 ℃ and strong light and natural light;

the single extraction rate of flavone by the above single method is not high, generally 40-60%, and it is required to be extracted for 3 times or more, and even after 3 times or more, the purity is only 30-50%; such as: the claim 1 of a bamboo leaf flavone beverage and its preparation method 202010982466.15, a bamboo leaf flavone beverage, characterized by that, it includes the following components in weight portion: 10-20 parts of a phyllostachys pubescens leaf flavone extract with the mass fraction of more than 50%, 20-40 parts of a double-peeling apple juice, 3-5 parts of a lemon concentrated juice and 40-60 parts of water; the phyllostachys pubescens flavone extract also contains 5-7% of phyllostachys pubescens polysaccharide by mass;

in summary, although the extraction method of octacosanol and bamboo leaf flavone has certain achievements, the method comprises the following steps:

firstly, the purity of the product in the market can only be used for health care functions of resisting fatigue and improving human body functions, and if the product is to achieve the effects of effectively reducing blood fat and preventing cardiovascular diseases, the purity is at least 90 percent, so that the further development of the product in the fields of food and medicine is limited, and therefore, the purity determines the physiological activity of a medicine; but also brings much trouble to further separation, and the subsequent refining process flow is relatively complicated to operate and takes time;

secondly, a plurality of functional beverages with single octacosanol or bamboo leaf flavonoid are added in the market at present, but the application range and the effect are limited by a single system mode; there is a limitation in the industrial use of functional beverages.

⒋ the invention researches a high-energy beverage refined by the synergistic combination of ultra-high-purity octacosanol-flavone composite deuterium-removed water and a preparation method thereof, and has strong application value

At present, certain prior art attempts are made on the application of single bamboo leaf flavone or octacosanol in the beverage processing technology, but the excellent properties of the bamboo leaf flavone or octacosanol are already self-evident, and the method of combined application of the bamboo leaf flavone or octacosanol has no more successful case at present; an ultra-high-purity octacosanol-flavone composite deuterium-removed water combined and synergistically refined high-energy beverage and a preparation method thereof are not reported yet.

Disclosure of Invention

Therefore, the high-energy beverage prepared by the synergistic refining of the combination of the ultra-high-purity octacosanol-flavone composite deuterium-removed water and the preparation method thereof are expected.

In order to achieve the purpose, the invention adopts the technical scheme that:

an ultra-high purity octacosanol-flavone composite deuterium-depleted water combined and co-refined high-energy beverage comprises the following components: 0.05-0.1 part of octacosanol, 0.12-0.2 part of flavone, 900-1000 parts of deuterium-removed water, 0.1-0.3 part of stabilizer, 0.3 part of compound emulsifier, 15-25 parts of water-soluble dietary fiber, 1.97 parts of compound electrolyte, 0.03-0.6 part of preservative and 0.05-0.2 part of sour agent;

the octacosanol is prepared by extracting octacosanol with the purity of more than or equal to 98% from fresh sugarcane filter mud;

the flavone is bamboo leaf flavone with the purity of more than or equal to 95 percent;

the tritium-removed water contains 50-100 ppm of deuterium.

Preferably, the compound emulsifier is a mixture of two or more of tween60, tween 80, molecular distillation monoglyceride, sucrose fatty acid ester, glyceryl monostearate, span 60 and span 80.

Preferably, the stabilizer is one or a mixture of more than two of acacia, fish gelatin, xanthan gum, pectin, sodium carboxymethyl cellulose, cyclodextrin and konjac gum. Preferably, the compound electrolyte is a mixture of 0.65 part of sodium citrate, 0.6 part of calcium lactate, 0.37 part of potassium chloride and 0.35 part of magnesium lactate.

Preferably, the water-soluble dietary fiber is one or a mixture of two of modified polydextrose, Partially Hydrolyzed Guar Gum (PHGG), wheat starch resistant dextrin, corn starch resistant dextrin and fructo-oligosaccharide.

Preferably, the sour agent is one or a mixture of two of citric acid, malic acid, lactic acid and cubilose acid.

Preferably, the preservative is one or a mixture of two of hydroxybenzoate, potassium sorbate and nisin.

Preferably, the preparation method of the high-energy beverage with the ultra-high purity octacosanol-flavone composite deuterium-depleted water combined synergistic refining comprises the following steps:

weighing 900-1000 parts of deuterium-removed water and 15-25 parts of water-soluble dietary fiber according to the parts by weight, adding the materials into a sealed reaction kettle, starting stirring at the rotating speed of 300-500 r/min, and stirring at normal temperature until the materials are completely dissolved; adding 0.05-0.1 part of octacosanol, 0.12-0.2 part of flavone, 0.1-0.3 part of stabilizer and 0.3 part of compound emulsifier, heating to 85 ℃, and emulsifying for 25min at a high shear speed of 1.8X104 r/min; cooling to 60-70 deg.C, homogenizing twice with homogenizer under 30-35Mpa for 3-4 min at an interval of 25 min; homogenizing for 1-2 min to obtain stable base material;

cooling to 50-55 ℃, weighing 1.97 parts of compound electrolyte, 0.03-0.6 part of preservative and 0.05-0.2 part of sour additive according to the weight part ratio, adding the mixture into the base material in the step, and continuously stirring for 30min at the rotating speed of 300-500 r/min;

sterilizing a system: UHT, 105-;

fourthly, filling: the high-energy beverage which is refined by the octacosanol-flavone composite deuterium-removed water combination is obtained.

Preferably, the ultrahigh-purity octacosanol-flavone composite deuterium-removed water combined and synergistically refined high-energy beverage is suitable for the fields of health care, beauty treatment, weight reduction, new-generation sports beverages, astronauts, divers, military high-energy beverages and the like by adjusting the change of components according to actual needs.

Preferably, the high-energy beverage prepared by the synergistic refining of the ultra-high-purity octacosanol-flavone composite deuterium-depleted water combination comprises the following components in percentage by weight:

the material selected for the method does not contain mammal animal components, does not have the hidden worry of common infectious diseases of people and livestock such as mad cow disease, foot and mouth disease and the like, and has rich sources and high safety;

the novel anti-counterfeiting bottle is prepared from Islamic authentication raw materials, and is not religious.

Has the beneficial effects that:

high-energy beverage prepared from ultra-high-purity octacosanol-flavone composite deuterium-depleted water through firstly pumping

A sports beverage called magic water (also called high-energy beverage) is silently created in the world at present, namely, traditional Chinese medicine components are added on the basis of the original sports beverage, so that the sports beverage has outstanding efficacy. The initial sports drink is a drink prepared by adding corresponding components into the drink in a targeted manner to supplement a large amount of water, sugar, salt, protein, vitamins and the like lost by a human body during high-intensity sports. Obviously, this is a formulation concept that is passively complementary; from modern medical theory, sports is an extreme physical activity, and after strenuous exercise, the human body not only loses the large amount of nutrients, but also some organs are subjected to overdraft of functions due to the strenuous exercise, are in an extremely fatigued state, and are difficult to recover quickly, such as the brain, the heart, the digestive system and the immune system. At this time, athletes can sleep badly at night, and have tachycardia, anorexia, poor gastrointestinal absorption function, susceptibility to diseases and the like; these in turn delay the overall recovery of body functions, which is not solved by simple supplementation of water, salt, etc.;

the invention adopts the combination of the ultra-high purity octacosanol-flavone composite deuterium-depleted water to supplement some special nutrient substances and cooperate with the refined high-energy beverage to pertinently regulate the organs in extreme fatigue states:

the bamboo leaf flavone with the purity of not less than 95% is prepared by a preparation method (patent application number: 202210302314.1) of the company for performing enhanced extraction of the total bamboo leaf flavone through a supercritical-microwave-assisted superfine homogenization negative pressure cavitation high pressure combined one-step method, and the analysis result of main nutrient components shows that the bamboo leaf flavone contains flavonoid compounds, active polysaccharides, phenolic acid compounds, anthraquinone compounds, manganese, zinc, selenium and other trace elements. The flavonoid compounds have obvious physiological activity effects of resisting ulcer, relieving spasm, resisting bacteria, resisting inflammation, reducing blood lipid, relieving pain, etc.; the active polysaccharide is sugar chain combined by sugar-shake bond, has curative effect on esophagus, bladder, kidney, lung, liver and uterine cancer of human body, can promote wound healing, lower blood pressure, detoxify and relieve fatigue, and has the main mechanism of enhancing liquid immunity of human body and improving the activity of leukocyte phagocyte; the phenolic acid compound has effects of sterilizing, refreshing, whitening skin, clearing heat, clearing pathogenic fire, nourishing yin, caring skin, refreshing, and relieving fatigue; the anthraquinone compounds have hemostatic, antibacterial, purgative, and diuretic effects; the trace elements are one of six indispensable nutrients of the human body, have the health-care functions of clearing away heat and improving eyesight, moistening lung and relieving cough, relaxing bowels and detoxifying, relieving alcoholism and soothing nerves, promoting cell metabolism, improving blood circulation and enhancing human immunity, and have good curative effects on various diseases such as tracheitis, hypertension, hyperlipidemia, diabetes and the like;

the functional beverage is characterized in that:

firstly, is a natural antioxidant

② antiaging effect

efficacy of losing weight

The excessive nutrition of a human body is converted into fat, and the fat stored in the body can be oxidized and denatured, which is one of the reasons that fat people are difficult to lose weight;

fourthly, its multifunctionality

Besides high-efficiency lipid oxidation resistance, the yellow pigment is a natural yellow pigment and has the functions of antibiosis, bacteriostasis, deodorization and aroma enhancement;

the experimental research discovers and verifies the influence of the purity of the bamboo leaf flavone on the concentration of high-density lipoprotein cholesterol in the serum of a rat:

the conventional bamboo leaf flavone group with the market purity of 50 percent and the bamboo leaf flavone group with the market purity of more than or equal to 95 percent are adopted, the bamboo leaf flavone is dissolved by 1 percent of Arabic gum to prepare suspension with corresponding dose, and the suspension is orally administrated once a day. See 5 d. Randomly divided into 2 groups by body weight and weighed 1 time per week. After 2 weeks, the rats were not fasted and blood was collected from the eyeballs, and serum HDL-C was measured;

TABLE 1 influence of bamboo leaf flavones on the serum HDL-C concentration of rats (mean value)

At the beginning of the experiment, the concentration of HDL-C in each group of serum has no obvious difference; after administration for 2 weeks, the bamboo leaf flavone with the purity of more than or equal to 95 percent can obviously improve the serum HDL-C concentration of a rat to be tested, the bamboo leaf flavone with the purity of 50 percent in the conventional market is not obvious in improving the serum HDL-C concentration (table 1), and researches show that the high-purity bamboo leaf flavone has the effect of improving the serum HDL-C concentration;

the experimental result provides a necessary theoretical basis for the development of bamboo leaf flavone lipid-lowering products.

The invention relates to a preparation method (patent application number: 202210303916.9) for extracting octacosanol with the purity of more than or equal to 98 percent from fresh sugarcane mud by adopting the ultrasonic-microwave assisted secondary recrystallization and distillation synergistic refining combination, which is a functional substance with anti-fatigue physiological activity, has various physiological effects of resisting fatigue, enhancing endurance and physical strength, improving cardiac muscle function, reducing blood fat and the like, and is a good natural health-care functional factor:

the sports equipment has the functions of improving physical exercise, such as enhancing endurance and physical strength, improving muscle strength, relieving muscle pain, reducing muscle friction and shortening muscle and nerve reaction time;

secondly, the medicine has the function of improving the physiological function of the human body, and is mainly characterized in that: increasing energy (including reaction sensitivity, stress ability); the reaction sensitivity is improved; improving the stress ability; strengthening heart function and oxygen delivery capacity, and reducing systolic blood pressure; improving the metabolism ratio of the organism, and the like;

the blood sugar level and the blood serum testosterone level of exercise-induced fatigue organisms can be improved, and myocardial damage is prevented;

fourthly, the immunity function of the organism can be improved, the biosynthesis of cholesterol is inhibited, the tolerance of the organism under the anoxic condition is facilitated, the oxygen conveying capacity is improved, and the operation capacity of the organism under the plateau condition is improved.

The mild dilatation and the cellular edema of the blood vessels of the heart and the brain tissues are relieved, the structure of the tissue cells is protected to a certain extent, and the potential anti-inflammatory and anti-tumor effects are also realized;

sixthly, the action of hormone is promoted, and the reproductive disorder of the human body is improved;

seventhly, the calcium and fat content of the blood is reduced, and the calcium-reducing agent is a calcitonin formation promoter and can be used for preventing and treating hypercalcemia osteoporosis, high cholesterol and high lipoprotein blood type diseases;

eighty percent can be used for preventing and treating Parkinson's disease in the old;

experimental research shows and verifies that the purity of octacosanol has the influence on the weight loss biological effect of a tail suspension simulated weightless rat:

it is well known that a series of physiological and biochemical changes occur in the body of an astronaut under weightless conditions, such as increased blood viscosity, increased blood lipid, bone calcium loss and the like;

in the experiment, rats are randomly divided into 3 groups, namely a tail suspension group (hereinafter called group A), a tail suspension group added with octacosanol with the conventional market purity of 80% (hereinafter called group B) and a tail suspension group added with octacosanol with the purity of more than or equal to 98% (hereinafter called group C); 8 rats per group, with a supply of 25mg/kg body weight per rat per day, for a period of 14 days; on the end of the experiment, blood and tissue samples were collected after the rats had fasted for 12 h; collecting heart blood by heart puncture, collecting trunk blood after head breakage, picking and weighing thymus and spleen, and separating and picking left hind limb femur; observing the weight of immune organs, the fluidity of red blood cell membranes, the biomechanical properties of femurs and the like:

the weights of the thymus and the spleen are first obtained

The weights of thymus and spleen of the rats in the group B are obviously lower than those of the rats in the group A, but the weight of thymus of the rats in the group C is 0.37g and is obviously higher than that of the rats in the group B by 0.24 g;

the result shows that the octacosanol with the purity of more than or equal to 98 percent can inhibit thymus atrophy caused by suspension weight loss simulation, and the thymus and the spleen are important immune organs of an organism, which prompts that the octacosanol with the purity of more than or equal to 98 percent has a certain protective effect on immune function reduction caused by suspension weight loss simulation;

indolizia membrane fluidity

The fluidity of erythrocyte membranes is generally expressed by the fluorescence polarization degree P value of erythrocyte membranes, and the larger the P value, the smaller the activity of hydrocarbon chains, the smaller the fluidity of the membranes and the larger the micro-viscosity of blood; conversely, the greater the membrane fluidity, the less the blood microsclerosis. After 14d suspension, the average values of the P values of the erythrocyte membranes of the rats in the B group and the C group are respectively 0.28 and 0.32, which are obviously higher than 0.26 of the rats in the A group, and the viscosity of the blood of the rats in the C group is reduced compared with the rats in the B group;

biomechanical parameters of the femur

TABLE 2 femur three-point bending test mechanics parameters (mean)

Biomechanical measurements of the femur obtained by the three-point bending test are given in table 2. After 14d of tail suspension, the maximum load, elastic limit load of the femur of the rats in group C is significantly higher than those in groups A and B. The result shows that the octacosanol with the purity of more than or equal to 98 percent adopted by the invention can obviously resist the reduction of the biomechanical property of the suspended rat femur;

TABLE 3 biomechanical Properties (mean) of rat femurs

Table 3 shows the results of the biomechanical property data of the femur calculated from the biomechanical and geometric parameters of the femur, which indicates that the octacosanol group with the purity of more than or equal to 98 percent adopted by the invention can obviously improve the biomechanical property of the femur of a suspended rat;

the experiment shows that the octacosanol with the purity of more than or equal to 98 percent adopted by the invention has the following functions on the caucasian rat: the weight of the thymus is obviously increased; improving the fluidity of the erythrocyte membrane of the simulated weightless rat; remarkably improving the biomechanical property of the thighbone; the octacosanol with the purity of more than or equal to 98 percent adopted by the invention can effectively resist certain adverse effects of a tail-suspended rat; no side effects of octacosanol were observed throughout the experiment; a series of physiological and biochemical changes of astronauts in vivo under the condition of zero gravity can be illustrated, and the health-care beverage or food added with the octacosanol with the purity of more than or equal to 98 percent can improve exercise endurance, reduce blood fat, improve blood viscosity, improve anoxia endurance and prevent osteoporosis, and can possibly become the health-care beverage or food component of astronauts.

⒊ the deuterium-removed water adopted by the invention has 25 to 100ppm of deuterium content, and the application in water for disease prevention and cure and beverage

In nature, the deuterium content is 1/7000 for hydrogen, the deuterium content of natural water is generally 150ppm for 145-. In fact, the deuterium-removed water is water which reduces deuterium to 10-130ppm by using modern technology;

along with the increasing high-carbohydrate food entering into the body nowadays, unhealthy life styles such as often staying up night, drinking alcohol overnight, eating sea and drinking, lack of sports for a long time and the like cause that excessive harmful substances, namely deuterium, exist in cells of modern people. Deuterium has an accumulation effect in vivo, is difficult to metabolize out by itself, and causes a plurality of adverse effects on DNA, heredity, metabolism and enzyme systems of human bodies;

the drinking of the deuterium-depleted water has a great deal of research abroad, and the biological effect of the deuterium-depleted water is proved to be a major breakthrough of the application research of the deuterium-depleted water in the field of foreign nuclear medicine and the field of water physiology in recent years:

the research of the Japanese research on deuterium-depleted water shows that: the deuterium-removed water environment can block the metabolism of cancer cells and induce the death of the cancer cells, and meanwhile, the attack of the medicine and the immunity of the organism to the cancer cells can take effect;

experiments of the American DDCENTER deuterium removal research center show that: the rest metabolic rate can be improved and the fatigue can be relieved by drinking the deuterium-depleted water;

the significance of deuterium on the regulation of biological metabolism is demonstrated in a book entitled "biological effects of cancer prevention and deuterium depleted water" by american experts L Á SZL Ó g, bosos professor, and positive effects of deuterium depleted water in tumor prevention and treatment are demonstrated in clinical trials, and it has also been found that: the deuterium-depleted environment has a positive effect on the diabetes treatment process, helping the patient to better control the blood glucose level;

fourth, Hungary HYD cancer research and drug development institutions have obtained ethical approval of deuterium-depleted water phase 2 clinical tests, and find that deuterium-depleted water can be applied to auxiliary articles for other tumors such as prostate cancer, breast cancer, lung cancer and pancreatic cancer;

fifthly, a research report on the anti-aging effect of the deuterium-removed water is published in "toxicology report" of 2012, and the result shows that the deuterium-removed water reverses the problem of life shortening caused by manganese (Mn) exposure, and the levels of DAF-16 and SOD-3 which play an important role in life extension in the nematode are restored;

sixthly, a research report that deuterium is high and the incidence rate of depression is possibly increased is published in a behavioural brain research in 2016, and the research finds that: deuterium depleted water increases the wakefulness EEG index and REM sleep duration, eliminating typical depressive state behavior, and may provide a new preventative strategy for depression;

for example, the famous longevity village hampshasa in the area of Gaoganese hardly obtains the common diseases of modern people, such as cancer, heart disease, abnormal blood pressure and the like. The investigation shows that: the deuterium content of the local daily drinking water and crops is less than 133ppm, far less than 150ppm in plain areas and 155ppm in equatorial areas;

for people who drink raw ketone and lose weight and shape, the deuterium water is also a choice which can help to achieve the effect.

The deuterium-depleted water can be made into non-alcoholic beverages, such as drinking water, mineralized water, magnetized water, soft drinks, functional drinks and the like, and can improve human health and life quality. At present, deuterium-depleted water products are marketed in the United states, Russia, Hungary, Ukrainian, Japan, Germany, France and the like, and the deuterium-depleted water is applied to various metabolic diseases and cancers;

in a word, water is a life source, the content of deuterium in water is an important standard for measuring the quality of water, the de-deuterium water is not only applied to scientific research, but also has the functions of activating immune cells, improving the basic metabolic level of an organism, resisting cell mutation, delaying senescence and the like, is more beneficial to the survival development and the multiplication of a life body, and has important significance for the health of human beings.

⒋ the invention adopts water-soluble dietary fiber, reduces sugar and substitutes sugar

One item of data from nielsen shows that of the five major product types with increased Z generation payments in the last year, low fat/low calorie/low sugar products are ranked first. Z generation believes that food and drink should not be a burden on the body, a healthier they wish to eat;

from the explosion of 0 sugar bubble water, we have seen that the concept of sugar reduction is rapidly expanding to many categories of bread, dairy products, chocolate candies, etc. However, while sweeteners can help reduce sugar intake, product taste is still sacrificed to some extent. How to reduce sugar healthily without sacrificing taste becomes a problem that the brand must solve when making sugar-reduced products in the future;

the invention relates to a solution scheme for replacing sugar with a water-soluble dietary fiber technology

The water-soluble dietary fiber adopted by the invention is not only a high-quality water-soluble dietary fiber, but also a unique low-calorie filler; replacing sugar with dietary fiber to reduce carbohydrate content and calorie, and using plant one-to-one sugar substitute; it is not only a good source of natural fiber, but also has no masking agent, no aftertaste and the same flavor as common sugar;

secondly, according to the hobbies and perceptions of different consumers, the scheme is adjusted, so that the effects of balancing and improving the overall flavor and taste experience can be achieved;

TABLE 4

Name of fiber product Balance	Modified polydextrose	Resistant dextrins	Hydrolysis of guar gum Thing (PHGG)	Resistant dextrins
					Raw materials	Corn starch	Wheat starch	Guar bean	Corn starch
Average molecular weight	2000	5000	15000	2000
					Average degree of polymerization	12	16	-	-
Fiber content (%)	Over 90	85	More than 80	More than 90 (85-95)
					Of fibre content Standard measuring method Method of	AOAC2000.11	AOAC2001.03	AOAC985.29	AOAC2001.03
Water solubility (%, w/w)	greater than 80	Greater than 70	Less than 5	Greater than 70
					Tolerance to stress	90g/d	30-45g/d	-	100g/d (no intake limit)
Heat quantity	1Kcal/g	2Kcal/g	0.2Kcal/g	0.5Kcal/g
					Stability in processing	Pasteurizing (70 deg.C, 30min) without significant hydrolysis; ultra high temperature Sterilizing for 6-10s without obvious hydrolysis. (at pH3 and 7)	Stable at pH2.5-7, and is suitable for various patients Processing technology; excellent in thermal processing stability	100 ℃ and pH5 Good lower stability	Almost no change in pH2 at 100 ℃; 120 ℃ and 2kg cm2 with almost no change for 10 minutes.
Moisture absorption property	Height of	The moisture absorption is started when the relative humidity is 80 percent	Is low with	Is low in
					Taste of food	Neutral or slightly sweet	Neutral property	Neutral property	Low sweetness, 10% of sucrose
Viscosity of the oil	High viscosity, higher than that of sucrose or sorbitol solution with the same concentration	Low viscosityDegree of rotation	Low viscosity	Extremely low; 30% solution viscosity at 30 ℃ of 15 cps

PHGG can obviously reduce the incidence rate of nosocomial influenza

Various researches on how to prevent the influenza caused by the new coronavirus and the new coronary pneumonia emerge endlessly, and academic reference is provided for effectively controlling the epidemic situation of the new coronary pneumonia;

a Japanese Single-center retrospective queue research article newly published in 4 months in 2021, The effect of partially hydrolyzed guar gum on preventive influenza infection (observation on The effect of partially hydrolyzed guar gum on preventing influenza infection), discusses The effect of PHGG on preventing influenza infection; the results show that: PHGG can affect the intestinal microenvironment, thereby contributing to the reduction of the incidence of influenza;

the clinical benefits of PHGG are still under further study, and it is found that it can improve blood sugar, reduce cholesterol and blood lipid, enhance mineral absorption, etc. in addition to improving intestinal health, and has been used by 45 countries to replace bracken and fat to produce low-calorie, low-sugar, low-fat, low-cholesterol and low-salt foods. In asian areas, it is more used as a fiber ingredient added to fiber-fortified beverages.

(II) resistant dextrin

Has excellent physiological functions:

(1) the preparation is beneficial to keeping normal and healthy blood sugar level and insulin level, delaying and inhibiting the digestion and absorption of small intestine to saccharides, improving the sensitivity of peripheral tissues to insulin and reducing the demand of insulin;

related studies have shown that daily intake of a certain amount of resistant dextrin is effective in preventing the symptoms of diabetes;

(2) changes the secretion of digestive tract hormone and the activity of intestinal tract digestive. The low molecular weight water-soluble dietary fiber can be continuously taken to reduce the concentration of serum cholesterol and neutral fat and the fat content in vivo;

(3) can adsorb bile acid, fat, etc. to reduce its absorption rate, and can reduce blood lipid and improve lipid metabolism of patients with various types of hyperlipidemia;

(4) the short-chain fatty acid is fermented in the large intestine to generate short-chain fatty acid with acid production more than that of the same dietary fiber, and the short-chain fatty acid can prevent the growth and the reproduction of cancer cells;

(5) the water absorption expansion can increase the volume of excrement and promote the intestinal tract movement, and has good prevention effect on diseases such as constipation, hemorrhoids, colon cancer and the like;

(6) helps to prevent dental caries;

until now, the soup products of American robol soup company, the soup bases of French dyne company, Nestle company, etc., the beverages and the dairy products, etc. also use the resistant dextrin in sequence, and the healthy beverages produced by Coca Cola company and Petasel Cola company which are the biggest beverage producing companies also adopt the material;

resistant dextrins are recognized by the FDA in the united states as GRAS substances and are not limited in intake. In Japan, resistant dextrin is widely used as a nutrition enhancer, and is recognized as a government-specified health food material by the Ministry of health and labor of Japan. In China, the Ministry of health [2012]516 has proposed to publish resistant dextrin as an unlimited amount of common food to be added to various foods; ③ modified polydextrose

Only part of the heat is involved in the metabolism of human body, and the generated heat is only IkCal/g (4.18 kJ/g); when it acts as a bulking agent to replace other sugars or fats in the food material, the calories provided are 25% of the sugars and 11% of the fat, respectively. After being taken into a human body, the metabolic process of the health-care food has no relation with insulin, so that the health-care food does not cause blood sugar change and is suitable for diabetics to eat;

the results of a clinical study performed on some healthy volunteers with 10g daily polydextrose according to Tomotari Mistuoka of the university of Tokyo, indicating that polydextrose prevents reabsorption of these steroids; after polydextrose administration, the viability of the glucokinase associated with colon cancer decreased, indicating that polydextrose can reduce the risk of colon cancer; in japan, modified polydextrose is considered as an important source of dietary fiber rice. Research shows that young women continuously drink 1 bottle of beverage containing 7-10 g of modified polydextrose every day for 5 days, so that the excrement of the young women is obviously softened, and constipation is effectively relieved;

in some countries such as china, japan, korea, and the like, modified polydextrose is more regarded as an important water-soluble dietary fiber;

fourthly, fructo-oligosaccharide

Can effectively reduce the amount of serum cholesterol, triglyceride and free fatty acid, and has good improving effect on a series of cardiovascular diseases such as hypertension and arteriosclerosis caused by high blood fat. The fructo-oligosaccharide is fermented by bacteria in large intestine to produce L-lactic acid, which can dissolve minerals such as calcium, magnesium, and ferrum, and promote absorption of minerals. Inhibiting the growth of harmful bacteria and regulating the balance in intestinal tracts; can promote the absorption and utilization of trace elements of iron and calcium to prevent osteoporosis; can reduce liver toxin, can generate anticancer organic acid in intestines, and has obvious cancer prevention function; and the taste is pure, fragrant, sweet and delicious, and has the fragrance similar to fat and the smooth and greasy feeling. The low heat value, because the fructo-oligosaccharide can not be directly digested and absorbed by human body, and can only be absorbed and utilized by intestinal bacteria, the heat value is low, the obesity can not be caused, and the weight-reducing effect can be indirectly realized. It is also a good sweetener for diabetics;

experiments prove that the fructo-oligosaccharide can promote the absorption rate of calcium to reach 70.8%. Therefore, the fructo-oligosaccharide can promote growth and development and prevent osteoporosis; meanwhile, the natural formation of vitamins B1, B2, B3, B6, B12 and folic acid can be promoted, so that the metabolism level of a human body is improved, and the immunity and the disease resistance are improved. Preventing and improving skin diseases caused by body toxicity, preventing facial sore, black speck, freckle, acne, senile plaque, and relieving skin aging. After absorbing fructo-oligosaccharide, bifidobacterium proliferates rapidly, inhibits putrefying bacteria such as escherichia coli, salmonella, clostridium and the like from generating effects, reduces the generation of toxic metabolites (such as indole and nitroso ammonia), simultaneously discharges the toxic metabolites out of the body rapidly, relieves the burden of the liver, plays a role in protecting the liver, and has obvious effects of preventing various chronic diseases, cancers and the like; the fructo-oligosaccharide is rarely decomposed by gastric acid and enzyme in digestive tract and is very difficult to be absorbed by human body;

according to the measurement, the calorific value of the fructo-oligosaccharide is less than 1.5Kcal/g, and the calorific value of the sucrose is 4.6Kcal/g, so that the fructo-oligosaccharide is not fat after being taken, and is an ideal functional sweetener with low calorific value. Fructooligosaccharides cannot be utilized by the mutant streptococci to form insoluble glucans to provide a site for oral microbial deposition, acidogenesis and erosion (tartar), and thus can prevent dental caries.

⒌ alternative emulsification and stabilization solution adopted by the invention

Experimental research discovers and verifies that various factors have influence on the stability of the high-energy beverage cooperatively refined by the ultra-high-purity octacosanol-flavone composite deuterium-free water composition:

effect of emulsifiers on beverage stability

The selection of the type and the dosage of the emulsifier plays an important role in the formation process of a stable emulsion system; the proper emulsifier can obviously reduce the surface tension between two phases, change the interface state of a system and be beneficial to stabilizing the uniform dispersion of liquid drops;

testing stability of one-factor oil-soluble emulsifier on beverage

Span 80 is selected as an example of the oil-soluble emulsifier, and experiments are carried out to investigate the stabilizing effect of the dosage on the beverage;

TABLE 5 Effect of oil-soluble emulsifiers on beverage stability results

② test of stability of one-way water-soluble emulsifier to beverage

The water-soluble emulsifier selects Tween 80 as an example, experiments are carried out, and the stabilizing effect of the dosage on the beverage is inspected;

TABLE 6 Effect of Water-soluble emulsifiers on beverage stability results

Influence of compound emulsifier on beverage stability

In order to improve the emulsification effect, the combination of different levels of oil-soluble emulsifier span 80 (A) and water-soluble emulsifier Tween 80 (B) is selected to carry out double-factor repeated tests, and the like as an example, the concentration of A is 0.05 part, 0.1 part and 0.15 part, and the concentration of B is 0.10 part, 0.15 part and 0.2 part. The centrifugal precipitation rate (%) is used as a test standard, and the smaller the centrifugal precipitation rate, the better. The test results are shown in Table 3:

TABLE 7 repeated test data and calculation table for two factors

As shown in the results of Table 7, it was confirmed that the optimum combination level of 0.2 parts Tween 80 and 0.1 part span 80 was the lowest in the centrifugal precipitation rate of the beverage. Therefore, the beverage prepared by the combined formula can achieve the best emulsification;

influence of a stabilizer on stability of a beverage

(ii) one-factor test of various stabilizers

3 types of common hydrocolloids were selected in this experiment: xanthan gum, sodium carboxymethylcellulose (CMC) and Arabic gum are taken as stabilizing agents to carry out a single-factor test of body state stability as an example, the beverage is observed to be greatly precipitated from rest under the same condition, and the type of the stabilizing agent required by the final test is determined according to the stabilizing effect;

TABLE 8 Single factor test for multiple stabilizers

As can be seen from Table 8, the 3 stabilizers xanthan gum, sodium carboxymethylcellulose (CMC) and acacia gum have better effect when being independently acted on the beverage, wherein the xanthan gum has the best effect and accords with the characteristic of strongest thickening effect;

② the influence of the compounding of several stabilizers on the stability of the beverage

In the single stabilizer comparison test, various stabilizers do not achieve ideal effects, so the test is further carried out by adopting the composite stabilizer. According to the results of the single-factor test, an orthogonal test was performed using three types of xanthan gum (0.4 parts, 0.6 parts, 0.8 parts), sodium carboxymethylcellulose CMC (0.2 parts, 0.4 parts, 0.6 parts), gum arabic (0.2 parts, 0.4 parts, 0.6 parts) as the stabilizer influencing factors, and the optimum compounding ratio and amount were determined using the centrifugal precipitation rate as an index. The precipitation rate was determined by averaging 3 replicates per treatment. The results of the test design are shown in Table 9

TABLE 9 determination of Compound stabilizers orthogonal test results

As can be seen from table 9, when the optimum combination of the stabilizers is 0.6 parts of xanthan gum, 0.2 parts of sodium carboxymethylcellulose CMC, and 0.4 parts of acacia gum, the centrifugal sedimentation rate of the beverage is the smallest; therefore, the beverage prepared by the combined formula can achieve the best stability;

the xanthan gum is used as a thickening agent, an emulsifying agent and a stabilizing agent, can increase the thick feeling of the beverage due to pseudoplasticity, stabilizes the suspension property of each component, thickens but does not have a sticky feeling, and has good fragrance releasing property; arabic gum is used as an emulsifying aid in the beverage, has good water solubility and emulsifying property, can increase the efficiency of an embedding object in the embedding process, enhances the mouthfeel, prevents precipitation and maintains the fragrance of the capsule; the sodium carboxymethylcellulose (CMC) has multiple functions of thickening, suspending, emulsifying, stabilizing and the like, has better water retention, dispersibility and thixotropy, can control the size and growth of ice crystals in the transportation and storage processes, and is used as a protective agent for beverages; therefore, the combination of the three can prevent the beverage from coagulating;

effects of other factors on beverage stability

Influence of high speed shearing conditions on beverage stability

The effect of high shear emulsification rate on beverage stability is also significant. Tests show that when the stirring speed is very low, the beverage has poor emulsification effect and obvious floating layering phenomenon, the stability of the beverage is in a trend of increasing in direct proportion along with the continuous increase of the stirring speed, but the increase gradually slows down when the emulsification speed reaches 1.8x104 r/min;

combining the factors, and selecting 1.8x104r/min as the optimal emulsification speed; the emulsification time is too short, the emulsification process is not sufficient, and the floating phenomenon of the beverage is serious; when the emulsification process is basically finished, the range of improving the stability is extremely small by prolonging the stirring time, and the value is not large in the actual production, so the emulsification time is preferably 25 min;

② influence of homogenization conditions on beverage stability

Homogenization is a common physical method for preventing settling and stratification of the beverage, and the choice of homogenization temperature and pressure directly affects the homogenization effect. In the test, the drink is homogenized under the pressure of 30-35Mpa and at the temperature of 60-70 ℃, and the test shows that: when the pressure is 30-35Mpa and the temperature is 60-70 ℃, the product has no layering, floating and precipitation phenomena when being homogenized twice. It can also be seen from the experiment that the higher the pressure, the higher the temperature, the more the homogenization times, the finer the particles, but the temperature and the pressure are too high. Too many homogenizers will consume too much production cost. Therefore, homogenizing twice at the pressure of 30-35Mpa and the temperature of 60-70 ℃, wherein the first homogenization is carried out for 3-4 min at an interval of 25 min; homogenizing for 1-2 min for the second time, and detecting by an electron microscope to obtain oil-in-water type droplets with the diameter of 3-5 μm. Can produce the beverage with good quality and good and stable tissue form;

the primary and secondary sequence of the influence of various factors on the stability of the beverage is as follows: high shear emulsification time > water-soluble emulsifier dosage > oil-soluble emulsifier dosage > stabilizer dosage;

influence of product stability

(1) System sterilization

UHT, 105-; can achieve better sterilization effect and can not cause obvious change of the properties of the beverage; but the phenomenon of layered floating of solute can be generated when the sterilization is carried out in a heating mode for a long time or is carried out;

(2) storage stability

The beverage is stored at the normal temperature for 30 days under the three storage conditions of 37 ℃, 4 ℃ and the normal temperature, wherein the storage time at the temperature of 37 ℃ and 4 ℃ is 2 weeks, and the experimental result shows that no floating and precipitation appear, and no obvious change is caused in the taste, so that the beverage is stable in process formula and has better storage stability.

Structure for subversing high-energy beverage

Breaking the conventional way, the method is a direct idea of technical innovation. Through the insight of consumer's demand, the innovation changes back high energy beverage structure, when bringing new-type health solution, has also promoted consumer's experience sense:

the preparation method (patent application number: 202210303916.9) for extracting octacosanol with the purity of more than or equal to 98 percent from fresh sugarcane filter mud and the preparation method (patent application number: 202210302314.1) for extracting bamboo leaf flavone with the purity of more than or equal to 95 percent in an enhanced manner by a supercritical-microwave-assisted ultra-fine homogenate negative pressure cavitation high pressure combined one-step method are all owned by the company and similar to ultrahigh purity and technology, and the aspect is not reported;

based on the known cognition in the industry, the physiological activity of a medicament is determined by the purity, the purity of flavone and octacosanol at least reaches 90 percent, and the effects of effectively reducing blood fat, preventing cardiovascular diseases and the like can be achieved;

the invention provides a synergistic combination of ultra-high-purity octacosanol-flavone composite deuterium-free water, cross comparison and integration are carried out according to the characteristics of each extract, the complementary and synergistic effects of refined high-energy beverage on organism regulation are reasonably and effectively enhanced and improved, and absorption and intracorporeal circulation are facilitated; the ultra-high purity extract is introduced into the beverage, the concept of passive supplementation is changed, the making concept of active conditioning beverage is created, the beverage has more excellent effects on the effects of resisting fatigue, improving the functions of human bodies, protecting health, beautifying, losing weight and the like, and the development of the fields of disease prevention and the like is further expanded; according to actual needs, the beverage is used in the fields of health care, new generation sports beverages, divers, astronauts and military development of military high-energy beverages by adjusting the change of components, and the like, and the aspect is not reported; the method is novel, provides theoretical reference for better development of high-energy beverages, becomes a manufacturing concept of the high-energy beverages in the future, and is more concerned by the society with the greater scope; the beverage has important practical significance for enriching the development of new generation functional high-energy beverage in China and promoting the health career of human beings; therefore, the invention has extremely wide and practical value;

secondly, the invention solves the problem of poor water solubility of octacosanol and flavone, does not generate any flavor and appearance problems in the beverage, and has better uniformity;

thirdly, the invention adopts water-soluble dietary fiber, and an alternative innovative solution for reducing sugar and substituting sugar;

the selected material is made of halal authentication raw materials, and religious distinction is avoided; the beverage is sugar-free, pigment-free, green, pure and healthy, and quality and safety guarantee is provided for the halal high-energy beverage;

fifthly, the refining process flow is relatively simple to operate and time-saving.

Detailed Description

The invention is further illustrated by the following examples: the technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.

Example 1

An ultrahigh-purity octacosanol-flavone composite deuterium-removed water combined and refined high-energy beverage comprises the following components: 0.05-0.1 part of octacosanol, 0.12-0.2 part of flavone, 900-1000 parts of deuterium-removed water, 0.1-0.3 part of stabilizer, 0.3 part of compound emulsifier, 15-25 parts of water-soluble dietary fiber, 1.97 parts of compound electrolyte, 0.03-0.6 part of preservative and 0.05-0.2 part of sour agent;

the octacosanol is octacosanol with the purity of more than or equal to 98 percent extracted from fresh sugarcane filter mud;

the deuterium-removed water contains 25-100 ppm of deuterium.

The preparation method comprises the following steps:

weighing 100ppm1000 parts of deuterium-removed water and 15 parts of water-soluble dietary fiber and adding the starch-resistant dextrin into a sealed reaction kettle according to the weight parts, starting stirring at the rotating speed of 500r/min, and stirring at normal temperature until the starch-resistant dextrin is completely dissolved; adding octacosanol 0.1 parts, flavone 0.2 parts, stabilizer 0.6 parts xanthan gum, sodium carboxymethylcellulose CMC 0.2 parts, acacia gum 0.4 parts, compound emulsifier 0.2 parts tween 80 and span 80 0.1 parts, heating to 85 deg.C, and emulsifying at high shear rate of 1.8 × 104r/min for 25 min; cooling to 70 deg.C, homogenizing twice with homogenizer under pressure of 35Mpa for 4min at an interval of 25 min; homogenizing for 2min to obtain stable base material;

cooling to 50-55 ℃, weighing 1.97 parts of compound electrolyte, 0.25 part of preservative and 0.2 part of sour agent according to the weight part ratio, adding the mixture into the base material in the step, and continuously stirring for 30min at the rotating speed of 500 r/min;

sterilizing a system: UHT, 105-;

fourth, filling: the high-energy beverage with ultrahigh-purity octacosanol-flavone composite deuterium-removed water combined synergistic refining is obtained.

Example 2

the deuterium-removed water has a deuterium content of 25-100 ppm.

The preparation method comprises the following steps:

weighing 50ppm950 parts of deuterium-depleted water and 20 parts of water-soluble dietary fiber (PHGG) according to the weight parts, adding the mixture into a sealed reaction kettle, starting stirring at the rotating speed of 400r/min, and stirring at normal temperature until the mixture is completely dissolved; adding 0.05 part of octacosanol, 0.2 part of flavone, 0.17 part of fish gelatin, 0.03 part of konjac glucomannan, 0.15 part of compound emulsifier, and 0.1 part of glycerin monostearate, heating to 85 ℃, and emulsifying for 25min at a high shear speed of 1.8x104 r/min; cooling to 65 deg.C, homogenizing twice with homogenizer under 33Mpa for 3.5min at an interval of 25 min; homogenizing for 1.5min to obtain stable base material;

cooling to 50-55 ℃, weighing 1.97 parts of compound electrolyte, 0.3 part of preservative and 0.1 part of sour agent in parts by weight as the base materials added in the process.

Sterilizing a system: UHT,105 ℃ and 121 ℃, 8 seconds;

Example 3

the deuterium-removed water contains 25-100 ppm of deuterium.

The preparation method comprises the following steps:

weighing 25ppm900 parts of deuterium-depleted water and 25 parts of water-soluble dietary fiber according to the weight parts, adding the weighed modified polydextrose into a sealed reaction kettle, starting stirring at the rotating speed of 300r/min, and stirring at normal temperature until the modified polydextrose is completely dissolved; adding 0.15 part of octacosanol, 0.12 part of flavone, 0.3 part of stabilizing agent, 0.045 part of compound emulsifier, 0.045 part of molecular distillation monoglyceride, 0.045 part of sucrose fatty acid ester and 0.18 part of Tween60, heating to 85 ℃, and emulsifying for 25min at a high shear rate of 1.8x104 r/min; cooling to 60 deg.C, homogenizing twice with homogenizer under 30Mpa for 3min at an interval of 25 min; homogenizing for 1min to obtain stable base material;

cooling to 50-55 ℃, weighing 1.97 parts of compound electrolyte, 0.03 part of nisin and 0.05 part of sour agent according to the weight part ratio, adding the lactic acid into the base material in the step, and continuously stirring for 30min at the rotating speed of 300 r/min;

sterilizing a system: UHT, 105-;

fourthly, filling: the high-energy beverage with ultrahigh-purity octacosanol-flavone composite deuterium-removed water combined synergistic refining is obtained.

Comparative example 1-1

the flavone is bamboo leaf flavone with the purity of more than or equal to 20 percent;

the deuterium-removed water contains 25-100 ppm of deuterium.

The preparation method comprises the following steps:

weighing 100ppm1000 parts of deuterium-removed water and 15 parts of water-soluble dietary fiber and adding the starch-resistant dextrin into a sealed reaction kettle according to the weight parts, starting stirring at the rotating speed of 500r/min, and stirring at normal temperature until the starch-resistant dextrin is completely dissolved; adding octacosanol 0.1 parts, flavone 0.2 parts, stabilizer 0.6 parts xanthan gum, sodium carboxymethylcellulose CMC 0.2 parts, acacia gum 0.4 parts, compound emulsifier 0.2 parts tween 80 and span 80 0.1 parts, heating to 85 deg.C, and emulsifying at high shear rate of 1.8 × 104r/min for 25 min; cooling to 70 deg.C, homogenizing twice with homogenizer under 35Mpa for 4min at an interval of 25 min; homogenizing for 2min to obtain stable base material;

cooling to 50-55 ℃, weighing 1.97 parts of compound electrolyte, 0.25 part of preservative and 0.2 part of sour additive in parts by weight, adding the weighed materials into the base stock in the step.

Sterilizing a system: UHT,105 ℃ and 121 ℃,10 seconds;

Comparative examples 1 to 2

the octacosanol is octacosanol with the purity of more than or equal to 80 percent extracted from fresh sugarcane filter mud;

the deuterium-removed water contains 25-100 ppm of deuterium.

The preparation method comprises the following steps:

weighing 1000 parts of deuterium-removed water and 15 parts of water-soluble dietary fiber and corn starch resistant dextrin according to the weight parts, adding the weighed materials into a sealed reaction kettle, starting stirring at the rotating speed of 500r/min, and stirring at normal temperature until the materials are completely dissolved; adding octacosanol 0.1 parts, flavone 0.2 parts, stabilizer 0.6 parts xanthan gum, sodium carboxymethylcellulose CMC 0.2 parts, acacia gum 0.4 parts, compound emulsifier 0.2 parts tween 80 and span 80 0.1 parts, heating to 85 deg.C, and emulsifying at high shear rate of 1.8 × 104r/min for 25 min; cooling to 70 deg.C, homogenizing twice with homogenizer under pressure of 35Mpa for 4min at an interval of 25 min; homogenizing for 2min to obtain stable base material;

sterilizing a system: UHT, 105-;

Comparative experiment 1

In order to observe the effect of the high-energy beverage on the concentration of high-density lipoprotein cholesterol in the serum of rats, a comparative experiment is carried out between the example 1 and the comparative example 1-1, and specific data references are given for the efficacy of the high-energy beverage in increasing the concentration of the high-density lipoprotein cholesterol (HDL-C) by detecting the level index of the high-density lipoprotein cholesterol (HDL-C) in the serum of rats:

selecting rats, providing basic feed, freely drinking water and diet, keeping the room temperature of the breeding room at 23 +/-5 ℃, keeping the relative humidity at 40-70%, and naturally changing the illumination. After the animals are observed and adapted for 5 days, 80 animals are selected for experiment and randomly divided into 4 groups, and 3 groups of the animals are fed with mixed type high-fat animal model feed: 20% of sucrose, 15% of lard, 1.2% of cholesterol, 0.2% of sodium cholate, 6.6% of casein, 0.5% of calcium hydrogen phosphate and 0.3% of stone powder are added to the basal feed, and drinking water of 2 groups of mice in 3 groups is replaced by the high-energy beverage prepared in example 1 and the high-energy beverage prepared in comparative example 1-1. Weigh 1 time per week, record food intake and calculate food utilization, test for 30 days. Blood is collected without fasting the eyeball in the middle period of the experiment, and the serum HDL-C is measured. At the end of the experiment, rats were bled without fasting the eyeballs and serum HDL-C was measured. And (4) carrying out anatomical observation, keeping the liver, the spleen, the kidney, the testis, the perirenal fat and the peritesticular fat, weighing, and calculating a corresponding index. Data were statistically analyzed using SPSS16.0 software. Data are expressed as mean ± standard error (Σ ± s), the significance test adopts one-factor analysis of variance, and the data are compared by a t-test method. The results are as follows:

TABLE 10 Effect on rat serum LDL-C concentration

Group of	Test set	Administration on day one	2 weeks after administration	4 weeks after administration
					Blank control group	HDL-C	0.45±0.06	0.47±0.07	0.47±0.10
Experimental control group	HDL-C	0.47±0.05	0.48±0.07	0.51±0.04
					Comparative example 1-1 test group	HDL-C	0.52±0.06	0.56±0.04	0.58±0.07
Example 1 test group	HDL-C	0.57±0.03	0.68±0.05	0.78±0.05

From the results, the invention can obviously improve the serum HDL-C concentration of a test rat when the bamboo leaf flavone with the purity of more than or equal to 95% is adopted, and the improvement of the serum HDL-C concentration is not obvious when the bamboo leaf flavone with the purity of 20% is adopted (Table 10). Research shows that the invention has the function of remarkably increasing the concentration of serum HDL-C when the high-purity bamboo leaf flavone is added.

Comparative experiment 2

In order to observe the effect of different purities of the extract of the high-energy drink on improving the swimming time and the anti-fatigue effect of the mice, a comparative experiment is carried out on the extract of the high-energy drink in example 1 and a comparative experiment 1-2, 30 rats are taken and randomly divided into a drink prepared in example 1 (hereinafter called drink A group), a drink prepared in comparative example 1-2 (hereinafter called drink B group), a deionized water blank control group (hereinafter called C group) and 10 rats in each group, the mice are subjected to gastric lavage for 10ml each, the administration dose is equivalent to 3g/kg, the administration is carried out for 15 days continuously and is carried out 30min after the last administration:

pole climbing test and load swimming test

Respectively placing the trained and screened mice on the upper ends of the vertically suspended smooth glass rods to enable muscles of the mice to be in a static tension state, recording the time of the mice from climbing a pole until the muscles are fatigued and fall to the ground without holding the glass rods, repeating the operation for 3 times, and terminating the test after the mice fall for the third time, wherein the time accumulated for 3 times is taken as pole climbing time(s);

the intragastric administration is continued for 5d, 30min after the last administration, and lead wire is loaded at the tail root according to 8% of the body weight; placing the mouse in a normal-temperature swimming box, and recording the time from the beginning of swimming to death of the mouse as swimming time (min);

deionized water blank control experiment: feeding deionized water into the stomach of a mouse, wherein the dose of each deionized water is 10ml, the dose is equivalent to 3g/kg, continuously feeding for 5 days, carrying out load swimming 30min after the last feeding, feeding lead skin at the tail part according to 8% of the weight of the mouse with normal-temperature water, swimming until the tail part is exhausted, and recording the time until the tail part is not immersed in water for 10 s;

the effect on the exercise endurance is as follows through practical test results:

group of	Dosage form	Time to climb the pole(s)	Swimming time ((min))
				Beverage A group	3g/kg	84	18.9
Beverage B group	3g/kg	48.7	14.4
				Group C	3g/kg	43.8	11.2

TABLE 11 influence of the test groups on the endurance of the mice (mean value)

② influence on biochemical indexes after swimming

When the drug is administrated for 15 days by gastric lavage, the tail root of a mouse is loaded with lead wires according to 3 percent of the weight of the mouse 30min after the drug is administrated, the mouse swims in water at normal temperature for 30min and then is taken out, the load is removed, the supraorbital venous plexus is taken out after the mouse is quiet for 15min, and the blood lactic acid content is measured by a p-hydroxybiphenyl color development method;

and (3) continuing intragastric administration for 5d for each group of mice, 30min after the last administration, putting the mice into normal-temperature water, swimming for 60min, taking out the mice, taking blood from supraorbital venous plexus, and measuring serum urea nitrogen by using a kit method. Then immediately killing the animal, taking the liver, washing with 0.9% NaCl solution, sucking dry by filter paper, and measuring the content of hepatic glycogen by an anthrone method;

results of

TABLE 12 Effect of octacosanol extracts on the biochemical indices of swimming mice (mean)

After the mice filled with the stomach 15d swim with a load, the blood lactic acid of the group A is obviously lower than that of the group B, which indicates that the exertion of the effect is probably related to the purity of the tested object;

the content of hepatic glycogen of group A is obviously increased compared with that of group B, which shows that the accumulation capacity of glycogen in animal bodies can be improved by the octacosanol with the purity of more than or equal to 98 percent adopted by the invention;

after 20d mice are fed with the medicine by gastric lavage for swimming, the serum urea nitrogen level is measured, the group A is obviously lower than the group B, and the comparison difference is extremely obvious, which shows that the octacosanol with the purity of more than or equal to 98 percent adopted by the invention can obviously reduce the serum urea nitrogen, and improve the body endurance and the adaptability to the movement load;

the tests show that the beverage A has obvious anti-fatigue effect when the group A adopts octacosanol with the purity of more than or equal to 98 percent, which shows that the high-energy beverage has the anti-fatigue health-care effect; the beverage B adopts octacosanol with low purity, and the anti-fatigue effect is obviously lower than that of the beverage A.

All the above examples are only for illustrating the technical solutions of the present invention and do not limit the embodiments, although the present invention is described in detail with reference to the foregoing examples, those skilled in the art should understand that: the technical solutions described in the above embodiments may still be modified, or some technical features may be equivalently replaced; the present invention is not limited to the above embodiments, but various modifications, substitutions and alternatives can be made without departing from the spirit and scope of the invention.

Claims

1. A high-energy beverage prepared by ultra-high purity octacosanol-flavone composite deuterium-depleted water combined synergistic refining is characterized in that: comprises the following components: 0.05-0.1 part of octacosanol, 0.12-0.2 part of flavone, 900-1000 parts of deuterium-removed water, 0.1-0.3 part of stabilizer, 0.3 part of compound emulsifier, 15-25 parts of water-soluble dietary fiber, 1.97 parts of compound electrolyte, 0.03-0.6 part of preservative and 0.05-0.2 part of sour agent;

the deuterium-removed water contains 25-100 ppm of deuterium.

2. The ultra-high purity octacosanol-flavone composite deuterium-depleted water combined synergistic refined high-energy beverage as claimed in claim 1, wherein: the compound emulsifier is a mixture of two or more of Tween60, Tween 80, sucrose fatty acid ester, molecular distillation monoglyceride, glyceryl monostearate, span 60 and span 80.

3. The ultra-high purity octacosanol-flavone composite deuterium-depleted water combined synergistic refined high-energy beverage as claimed in claim 1, wherein: the stabilizer is one or more of acacia, fish gelatin, xanthan gum, pectin, sodium carboxymethylcellulose CMC, cyclodextrin, and konjac gum.

4. The ultra-high purity octacosanol-flavone composite deuterium-depleted water combined synergistic refined high-energy beverage as claimed in claim 1, wherein: the compound electrolyte is a mixture of 0.65 part of sodium citrate, 0.6 part of calcium lactate, 0.37 part of potassium chloride and 0.35 part of magnesium lactate.

5. The ultra-high purity octacosanol-flavone composite deuterium-depleted water combined and co-refined high-energy beverage of claim 1, wherein: the water-soluble dietary fiber is one or a mixture of two of modified polydextrose, Partially Hydrolyzed Guar Gum (PHGG), wheat starch resistant dextrin, corn starch resistant dextrin and fructo-oligosaccharide.

6. The ultra-high purity octacosanol-flavone composite deuterium-depleted water combined synergistic refined high-energy beverage as claimed in claim 4, wherein: the sour agent is one or a mixture of two of citric acid, malic acid, lactic acid and cubilose acid.

7. The ultra-high purity octacosanol-flavone composite deuterium-depleted water combined and co-refined high-energy beverage of claim 1, wherein: the preservative is one or a mixture of two of hydroxybenzoate, potassium sorbate and nisin.

8. The ultra-high purity octacosanol-flavone composite deuterium-depleted water combined synergistic refined high-energy beverage as claimed in claims 1-7, wherein: according to the actual requirement, the beverage is suitable for the fields of health care, beauty treatment, weight reduction, new generation sports beverages, divers, astronauts, military high-energy beverages and the like by adjusting the change of components.

9. The ultra-high purity octacosanol-flavone composite deuterium-depleted water combined synergistic refined high-energy beverage as claimed in claims 1-7, wherein:

the novel anti-counterfeiting product is made of Islamic certification raw materials and has no religious distinction.

10. The preparation method for preparing the ultrahigh-purity octacosanol-flavone composite deuterium-depleted water combined and synergistically refined high-energy beverage as claimed in claim 1, is characterized in that: the method comprises the following steps:

sterilizing a system: UHT, 105-;