CN114916431B - Indoor cultivation method for promoting cotton generation-adding breeding - Google Patents
Indoor cultivation method for promoting cotton generation-adding breeding Download PDFInfo
- Publication number
- CN114916431B CN114916431B CN202210642721.7A CN202210642721A CN114916431B CN 114916431 B CN114916431 B CN 114916431B CN 202210642721 A CN202210642721 A CN 202210642721A CN 114916431 B CN114916431 B CN 114916431B
- Authority
- CN
- China
- Prior art keywords
- cotton
- seeds
- cultivation method
- indoor cultivation
- water content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
- A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- A01H1/1225—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold or salt resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
- A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention belongs to the technical field of crop breeding and cultivation, and particularly relates to an indoor cultivation method for promoting cotton generation-added breeding. On the basis of dry sowing and wetting of cotton seeds, drought stress and high-temperature stress are combined, the biological characteristics of cotton which are temperature-loving and drought-tolerant are fully utilized, the promotion effect of high temperature and drought on the growth and development of cotton is maximized, and the phenomena of bud boll shedding and sterility caused by excessive environmental stress are avoided, so that the growth and development of cotton are effectively accelerated, the breeding period of cotton is shortened, and the breeding efficiency is improved.
Description
Technical Field
The invention belongs to the technical field of crop breeding and cultivation, and particularly relates to an indoor cultivation method for promoting cotton generation-added breeding.
Background
Cotton is the most important raw material for textile work as the most important fiber crop in the world, and has an important position in economic development of various countries. The contribution rate of improvement and replacement of cotton varieties to cotton yield is about 45%. In recent years, the yield per unit of cotton is not high, and the main reason is that the new variety does not bring remarkable yield-increasing benefit, so that the breeding of the new cotton variety needs to be further accelerated. The cotton has a long growth period, generally 120-140 days, and the climate conditions in China determine that only one generation can be planted in one year, and the generation can only reach two generations in one year even if the south breeding is carried out, so that the breeding speed of new varieties is severely restricted.
Meanwhile, cotton is domesticated by human for thousands of years, and the domestication process improves important agronomic characters of the cotton and causes great reduction of genetic diversity and deletion of dominant gene resources. In the process of cotton de novo domestication based on modern biotechnology such as gene editing and the like, the method for rapidly adding generations of cotton with various functions and stable properties is required to be created as technical support. Therefore, creating a technology capable of accelerating the growth, development and iteration of cotton has become a key problem to be solved urgently.
The cotton has the biological characteristic of adapting to artificial regulation, and the artificial climate chamber can utilize the optimal environment to maximize the growth and development speed of crops due to the controllable environmental conditions, and realize indoor annual breeding. Although research is carried out on methods for accelerating the growth period of cotton at present, the problem that the growth period is too long still exists. Therefore, the exploration of an indoor cultivation method for further accelerating the growth and development of cotton and accelerating the generation turnover has important significance for shortening the breeding period of cotton, saving the breeding cost and improving the breeding efficiency.
Disclosure of Invention
The invention aims to provide an indoor cultivation method for promoting cotton generation-added breeding, which can effectively accelerate the growth and development of cotton, shorten the breeding period of the cotton, further shorten the growth period of the cotton to about 85 days, breed about 4.3 generations every year and improve the breeding efficiency.
The invention provides an indoor cultivation method for promoting cotton generation-adding breeding, which comprises the following steps: and (3) sowing the cotton seeds in a dry mode, and wetting the cotton seeds, and performing drought stress and high-temperature stress after the seeds emerge until the seeds are harvested.
Preferably, the dry seeding and wet seeding comprises the steps of seeding the cotton seeds in a dry planting matrix, supplementing water until the relative water content of the planting matrix is 75-85%, and keeping the relative water content of 75-85% until the seeds emerge.
Preferably, the drought stress comprises that the relative water content of the planting matrix is reduced to 50-60% without watering after the seeds emerge, and the relative water content of 50-60% is maintained until the seeds are harvested.
Preferably, the high-temperature stress comprises the steps that the cotyledons of the cotton seedlings are flattened to 4-6 true leaves, the culture temperature in the daytime is adjusted to 29-31 ℃, and the culture temperature at night is adjusted to 25-27 ℃; adjusting the culture temperature to 34-36 ℃ in the daytime and 29-31 ℃ at night when the seeds are harvested from 4-6 true leaves.
Preferably, during the dry sowing and wet sowing process, the Hoagland nutrient solution is used for supplementing water.
Preferably, during the drought stress, the relative water content of 50-60% is maintained by using Hoagland nutrient solution and water, and the daily supplement amount of the Hoagland nutrient solution is 50-100 mL per plant.
Preferably, the concentration of the Hoagland nutrient solution is 0.8-1.2M.
Preferably, the planting substrate comprises medium soil, vermiculite and perlite, and the volume ratio of the medium soil to the vermiculite to the perlite is 1: (0.8-1.2): (0.8-1).
Preferably, the particle size of the medium soil is 1.5-2.5 mm, the particle size of the vermiculite is 1-3 mm, and the particle size of the perlite is 3-6 mm.
Preferably, the sowing depth is 2-3 cm.
Has the advantages that:
the invention provides an indoor cultivation method for promoting cotton generation-adding breeding, which combines drought stress and high-temperature stress on the basis of dry sowing and wetting of cotton seeds, fully utilizes the biological characteristics of cotton which is temperature-loving and drought-enduring, maximizes the promotion effect of high temperature and drought on the growth and development of cotton, and simultaneously avoids the generation of boll shedding and sterility phenomena caused by excessive environmental stress, thereby effectively accelerating the growth and development of cotton, shortening the breeding period of cotton and improving the breeding efficiency.
Secondly, the cultivation method of dry sowing and wet sowing can avoid the traditional germination accelerating treatment and seedling stage transplanting, shorten the seedling revival stage and achieve the technical effect of uniform and uniform seedlings.
Detailed Description
The invention provides an indoor cultivation method for promoting cotton generation-adding breeding, which comprises the following steps: and (3) sowing the cotton seeds in a dry mode, and wetting out the cotton seeds, and performing drought stress and high-temperature stress after the seeds emerge until the seeds are harvested. The indoor cultivation method provided by the invention has no special limitation on the specific implementation area, and the technical scheme of the invention can be implemented in any area.
The dry seeding and wetting method preferably comprises the steps of seeding the cotton seeds in the dry planting substrate, supplementing water until the relative water content of the planting substrate is 75-85%, and keeping the relative water content of 75-85% until the seeds emerge.
Before the cotton seeds are sown in the dry planting matrix, the preparation of the planting matrix is preferably included. The preparation of the planting base according to the invention preferably comprises sterilization and mixing. The planting substrate preferably comprises medium soil, vermiculite and perlite, and the volume ratio of the medium soil, the vermiculite and the perlite is preferably 1: (0.8-1.2): (0.8 to 1), more preferably 1:1:1. the particle size of the medium soil is preferably 1.5-2.5 mm, and more preferably 2mm; the particle size of the vermiculite is preferably 1-3 mm, and more preferably 2mm; the perlite preferably has a particle size of 3 to 6mm, more preferably 4.5mm. The invention preferably mixes the medium soil with the vermiculite and the perlite after sterilizing; the sterilization temperature is preferably 130-170 ℃, and more preferably 150 ℃; the time for the sterilization is preferably 18 to 30 hours, more preferably 24 hours. The sterilization of the present invention can kill weed seeds and pathogenic bacteria. The medium soil has the characteristics of ventilation, water permeability, water retention and fertilizer retention.
After the preparation of the planting substrate is completed, the present invention preferably spreads the cotton seeds evenly around the center point of the planting device. The number of the cotton seeds to be sown and the planting device are not particularly limited, and those skilled in the art can adjust the number and the planting device according to the planting experience and the planting device, and the following description will be made in terms of the planting manner in the embodiment, but the embodiment cannot be regarded as the full protection scope of the invention, in the embodiment of the invention, 2.2kg of planting substrate is placed in a flowerpot with the bottom diameter of 20cm, the upper diameter of 23cm and the height of 20cm, a tray with the diameter of 25cm and the height of 2cm is placed at the bottom of the flowerpot, and 3-5 cotton seeds are sown uniformly around the center point of the flowerpot in each flowerpot. The sowing depth of the sowing of the invention is preferably 2-3 cm, more preferably 2.5cm.
After the sowing is finished, the invention preferably replenishes water until the relative water content of the planting substrate is 75-85%, and maintains the relative water content of 75-85% until the seeds emerge. The relative water content of the planting substrate of the invention is preferably 80%. In the present invention, the water supplement is preferably performed by using a Hoagland nutrient solution, and the concentration of the Hoagland nutrient solution is preferably 0.8 to 1.2M, and more preferably 1M. The calculation formula of the relative water content is preferably as follows:
wherein RWC represents the relative water content; GA represents the actual weight of substrate and water when weighed; GS represents the weight of dry substrate; GW represents the total weight of matrix and water when the dry matrix is saturated with water.
After the seeds emerge, the method carries out drought stress and high temperature stress on the cotton seedlings until the seeds are harvested. The drought stress of the invention preferably comprises that the relative water content of the planting matrix is reduced to 50-60% without watering after the seeds emerge, and the relative water content of 50-60% is maintained until the seeds are harvested. The relative water content of the drought stress of the present invention is preferably 55%. The present invention preferably employs Hoagland nutrient solution and water to maintain the relative water content of 50-60%. The daily supplement amount of the Hoagland nutrient solution is preferably 50-100 mL, and more preferably 100mL; the concentration of the Hoagland nutrient solution is the same as that in the dry sowing and wet sowing process, and the details are not repeated. The amount of water required for maintaining the relative water content of the planting substrate of 50-60% is not particularly limited, and the relative water content of the planting substrate can reach 50-60% by matching with Hoagland nutrient solution.
The method preferably further comprises the step of carrying out high-temperature stress on the cotton seedlings until the seeds are harvested while carrying out the drought stress. The high-temperature stress of the invention preferably comprises the steps that the cotyledons of the cotton seedlings are flattened to 4-6 true leaves, preferably to 5 true leaves, the culture temperature in the daytime is adjusted to 29-31 ℃, and the culture temperature at night is adjusted to 25-27 ℃; the daytime culture temperature is more preferably 30 ℃ and the nighttime culture temperature is more preferably 26 ℃. When the cotton seedlings reach 4-6 true leaf stages (bud formation begins), the invention preferably also comprises adjusting the daytime culture temperature to 34-36 ℃ and the nighttime culture temperature to 29-31 ℃; the daytime culture temperature is more preferably 35 ℃ and the nighttime culture temperature is more preferably 30 ℃.
In the present invention, the third day and night temperatures from the sowing of cotton to the flattening of the cotton seedling cotyledons to 4 to 6 are preferably 24 to 28 ℃ and 20 to 24 ℃, more preferably 26 ℃ and 22 ℃, respectively. The light quantum flux density of the invention in the whole cotton growing process is preferably 900-1000 mu mol.m -2 ·s -1 More preferably 1000. Mu. Mol. M -2 ·s -1 。
The illumination period from sowing to seed harvesting is preferably 11-12L: 13 to 12D, more preferably 12L:12D; the relative humidity of air is preferably 50 to 60%, and more preferably 55%. On the basis of dry sowing and wetting of cotton seeds, drought stress and high temperature stress are combined, the biological characteristics of cotton which are temperature-loving and drought-enduring are fully utilized, the promotion effect of high temperature and drought on the growth and development of cotton is maximized, especially under the conditions of the drought stress and the high temperature stress, the growth and development of cotton can be better promoted, the growth period of the cotton is shortened, and the annual breeding generation number is increased.
After the drought stress and the high temperature stress are finished, the method preferably performs seed harvesting. The invention preferably harvests the seed cotton 2 days after the cotton bolls are cracked, dries and ginzes, and stores for later use.
The cotton variety is not particularly limited, and cotton varieties in the field can all adopt the technical scheme of the invention to realize cotton additive breeding, for example, the technical scheme of the invention is described by Xinluzao No. 33 and Xinluzao No. 45 in the embodiment of the invention, but the technical scheme cannot be regarded as the full protection scope of the invention.
Besides the cultivation steps defined in the above technical scheme, the present invention does not specifically limit other steps in the cotton cultivation process, and a person skilled in the art can perform routine adjustment according to the planting experience, but the technical scheme derived by adding the routine adjustment steps also belongs to the protection scope of the present invention.
In order to further illustrate the present invention, the following embodiments are described in detail, but they should not be construed as limiting the scope of the present invention.
Example 1
An indoor cultivation method for promoting cotton generation-adding breeding comprises the following steps:
the cotton varieties Xinluzao No. 33 and Xinluzao No. 45 are used as test materials and are respectively cultivated in artificial climates of key laboratories of ecological agriculture of the Bingqu oasis produced and constructed in Xinjiang.
1. Preparing a planting matrix: sieving loam soil in a wheat test field of an agricultural test station of the university of river stone by using a 2mm sieve, and drying the loam soil in an oven at 150 ℃ for 24 hours to kill weed seeds and various pathogens; mixing with vermiculite (particle size of 2 mm) and perlite (particle size of 4.5 mm) at equal volume ratio to obtain planting matrix, placing 2.2kg of matrix in a flowerpot with bottom diameter of 20cm, upper diameter of 23cm and height of 20cm, and placing a tray with diameter of 25cm and height of 2cm at the bottom of the flowerpot.
2. Dry seeding and wet seeding: the pots and trays with the dry planting medium were placed in a climatic chamber, 4 seeds were evenly sown around the center point in each pot, with a sowing depth of 2.5cm. And (3) after sowing, pouring 1M Hoagland nutrient solution into a tray at the bottom of the flowerpot until the relative water content of the planting matrix in the flowerpot reaches 80%. The calculation formula of the relative water content of the planting substrate in the flowerpot is as follows:
wherein RWC represents the relative water content; GA represents the actual weight of substrate and water when weighed; GS represents the weight of dry substrate; GW represents the total weight of matrix and water when the dry matrix is saturated with water.
3. Water stress during the whole growth period: after the seeds emerge, watering is not carried out, the relative water content of the planting matrix in the flowerpot is naturally reduced to 55% of the drought state, then the relative water content in the planting matrix is calculated by a weighing method every day, and 100ml of Hoagland nutrient solution and water are supplemented into the tray to maintain the relative water content at 55% of the drought state.
4. High temperature stress during the whole growth period: after the cotton seeds emerge and the cotyledons are flattened, the temperature of the climate chamber is adjusted to 30 ℃ and 26 ℃ day and night respectively. When 5 true leaves, namely cotton, begin to bud, the temperature of the climate chamber is adjusted to 35 ℃ and 30 ℃ day and night respectively.
Day and night temperatures from cotton sowing to cotyledon flattening are respectively 26 ℃ and 22 ℃, and day and night temperatures from cotton seedling cotyledon flattening to 5 are respectively 30 ℃ and 25 ℃. The light quantum flux density of cotton in the whole growth process is 1000 mu mol.m -2 ·s -1 。
5. Harvesting seeds: and when the cotton bolls crack, harvesting the first generation seed cotton 2 days, airing and ginning, and storing for later use.
The illumination period from the cotton seedling stage to the seed harvest is 12L:12D; the relative humidity of the air was 55%.
Comparative example 1
The indoor cultivation method in example 1 was employed except that the whole-growth-period high-temperature stress in step 4 was not performed.
Comparative example 2
The indoor cultivation method in example 1 was employed except that the water stress in the whole growth period in step 3 was not performed.
Comparative example 3
The indoor cultivation method of example 1 was used, except that, in the course of performing step 3, the relative water content of the planting base from sowing to harvesting was 75%; in the process of step 4, the day and night temperatures from sowing to budding were 26 ℃ and 22 ℃, respectively, and the day and night temperatures from budding to seed harvesting were 30 ℃ and 24 ℃, respectively.
Comparative example 4
The indoor cultivation method of example 1 was employed except that the water stress during the whole growth period in step 3 was performed, the relative soil moisture content was controlled to be in a drought state of 35%.
Comparative example 5
The indoor cultivation method of example 1 was used, except that when the high temperature stress was applied during the whole growth period in step 4, the temperature in the climate chamber was adjusted to 38 ℃ and 30 ℃ day and night, respectively, after 5 true leaves and cotton began to bud.
Application example 1
The cotton growth period of example 1 and comparative examples 1-5 was counted, and the results are shown in Table 1:
TABLE 1 growth period Difference in Cotton of example 1, comparative examples 1-3
Note: the values are mean values. + -. Standard error, and the same species has different letters in the same row to indicate that the level difference of P <0.05 is obvious, as follows. Representative of this data not being available in the test.
The growing period days are important indexes reflecting cotton generation-adding efficiency, and can be seen from table 1: under the high-temperature drought interactive condition (example 1) in the technical scheme of the invention, the growth period of different varieties of cotton is obviously shorter than that of cotton under the normal growth condition (comparative example 3) in a greenhouse and is also obviously higher than that of single high-temperature condition (comparative example 2) and drought condition (comparative example 1). Thus, under high temperature drought-interactive conditions (example 1), the number of annual indoor cycles is also significantly higher than under single high temperature conditions (comparative example 2) and drought conditions (comparative example 1). Meanwhile, the too low soil moisture content in comparative example 4 causes the cotton to grow slowly and the cotton buds cannot bloom normally, and the too high environmental temperature in comparative example 5 causes the cotton to drop young bolls within 3-5 days after blooming. It is indicated that both excessive drought stress and excessive temperature can cause cotton to fail to bear bolls, and are not suitable for being used as environmental conditions for rapid growth and development of cotton. Since comparative example 4 and comparative example 5 failed to produce mature bolls, comparison of comparative example 4 and comparative example 5 was omitted below. In conclusion, the whole-growth-period high-temperature and water stress technology (example 1) used in the invention can further accelerate the growth and development of cotton on the basis of avoiding cotton bud boll shedding and sterility caused by extreme environmental stress, and can enable the cotton to breed 4.3 generations annually.
Application example 2
The cotton of example 1, comparative examples 1 to 3 was measured for differences in single boll weight, seed number and thousand kernel weight, and the results are shown in Table 2:
TABLE 2 Single boll weight, seed number and thousand kernel weight differences for the cotton of example 1, comparative examples 1-3
The single boll weight is an important index for reflecting the cotton boll development, and Table 2 shows that the cotton single boll weight and the number of single boll seeds are obviously smaller than those of the cotton under the normal growth condition (comparative example 3) and the high temperature condition (comparative example 2) under the high-temperature drought interaction condition of the two varieties (example 1), but the cotton single boll weight and the number of single boll seeds are not obviously different from those of the cotton under the drought condition (comparative example 1). The thousand seed weight under high temperature drought conditions (example 1) was significantly lower than the other three comparative examples. The weight of single boll, the number of single boll seeds and the thousand kernel weight of each two different varieties are the lowest under the high-temperature drought interactive condition (example 1), however, when the accelerated breeding is carried out, the next generation of breeding work can be carried out by only one seed, while the cotton can still produce more than 3g of seed cotton under the high-temperature drought interactive condition (example 1), and the single boll can produce 19-23 mature cotton seeds without influencing the next breeding process.
The reduction of the weight of a single boll and the thousand-kernel weight of the seeds is the negative influence brought by the rapid growth and development of cotton. After the growth rate and the yield and the quality are balanced, the number and the quality of seeds can be sacrificed to a certain extent to replace the faster growth and development rate of cotton and peanuts, so that the main purpose of quickly replacing cotton is achieved.
Application example 3
Comparing the seed development of cotton progeny of example 1, comparative examples 1-3, the results are shown in table 3:
TABLE 3 Germination Difference in seeds of cotton progeny in example 1, comparative examples 1 to 3
After the cotton seeds are harvested, the smooth cultivation of the offspring can be effectively realized only by ensuring a certain germination rate. As can be seen from table 3, the germination potential of the new yozao 33 seed does not significantly differ from that of the seed under the drought condition (comparative example 1) under the interaction condition of high temperature and water stress (example 1) of the present invention, but is significantly lower than that of the seed under the high temperature condition (comparative example 2) and the normal growth condition (comparative example 3), and the germination rate and germination index of the seed do not significantly differ from those of the drought condition (comparative example 1) and the high temperature condition (comparative example 2). New grandma No. 45 under the high temperature and water stress interaction condition (example 1), the germination potential of the seeds was significantly lower than that of the drought condition (comparative example 1), the high temperature condition (comparative example 2) and the normal growth condition (comparative example 3), while the germination rate and germination index were significantly lower than that of the high temperature condition (comparative example 2) and were not significantly different from those of the drought condition (comparative example 1) and the normal growth condition (comparative example 3). It is shown that the germination of different cotton varieties is mainly affected by drought, but the seeds can also keep a certain germination rate under the drought condition. That is to say, the germination rates of the progeny seeds generated by the high-temperature drought technology in the whole growth period are all over 80 percent, the germination rates are higher, and the requirements on the germination rates of the seeds during the breeding of the additional generations can be met.
The technical scheme provided by the invention can accelerate the development of cotton, shorten the development period of the cotton, shorten the breeding period of the cotton and improve the breeding efficiency under the condition of ensuring the basic quality of the cotton and the development of progeny seeds.
Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.
Claims (7)
1. An indoor cultivation method for promoting cotton generation-adding breeding is characterized by comprising the following steps: sowing cotton seeds in a dry mode, and wetting the cotton seeds, and performing drought stress and high-temperature stress after the seeds emerge until the seeds are harvested;
the dry seeding and wet seeding comprises the steps of seeding cotton seeds in a dry planting matrix, supplementing water until the relative water content of the planting matrix is 75-85%, and keeping the relative water content of 75-85% until the seeds emerge;
the drought stress comprises that after the seeds emerge, watering is not carried out, so that the relative water content of the planting matrix is reduced to 50-60%, and the relative water content of 50-60% is maintained until the seeds are harvested;
the high temperature stress comprises the step of flattening the cotton seedling cotyledons to 4 to 6 true leaves, adjusting the daytime culture temperature to 29 to 31 ℃, and adjusting the nighttime culture temperature to 25 to 27 ℃; and (3) harvesting the seeds in the true leaf period of 4-6 leaves, and adjusting the culture temperature in the daytime to be 34-36 ℃ and the culture temperature at night to be 29-31 ℃.
2. The indoor cultivation method according to claim 1, wherein moisture is supplemented with Hoagland's nutrient solution during the dry sowing and wet sowing.
3. The indoor cultivation method according to claim 1, wherein the relative water content of 50 to 60% is maintained by using Hoagland nutrient solution and water during the drought stress, and the supplement amount of the Hoagland nutrient solution per day is 50 to 100mL per plant.
4. The indoor cultivation method according to claim 2 or 3, wherein the concentration of the Hoagland nutrient solution is 0.8 to 1.2M.
5. The indoor cultivation method according to claim 1, wherein the planting substrate comprises medium soil, vermiculite and perlite, and the volume ratio of the medium soil, the vermiculite and the perlite is 1: (0.8 to 1.2): (0.8 to 1).
6. The indoor cultivation method as claimed in claim 5, wherein the particle size of the medium soil is 1.5 to 2.5mm, the particle size of the vermiculite is 1 to 3mm, and the particle size of the perlite is 3 to 6mm.
7. The indoor cultivation method as claimed in claim 1, wherein the seeding depth is 2 to 3cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210642721.7A CN114916431B (en) | 2022-06-08 | 2022-06-08 | Indoor cultivation method for promoting cotton generation-adding breeding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210642721.7A CN114916431B (en) | 2022-06-08 | 2022-06-08 | Indoor cultivation method for promoting cotton generation-adding breeding |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114916431A CN114916431A (en) | 2022-08-19 |
| CN114916431B true CN114916431B (en) | 2023-03-24 |
Family
ID=82812066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210642721.7A Active CN114916431B (en) | 2022-06-08 | 2022-06-08 | Indoor cultivation method for promoting cotton generation-adding breeding |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114916431B (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107135782B (en) * | 2017-05-31 | 2020-06-02 | 中国农业科学院棉花研究所 | Cultivation method of southern Xinjiang membraneless cotton |
| CN109105252A (en) * | 2018-08-25 | 2019-01-01 | 中国农业科学院棉花研究所 | A kind of method of quickly breeding new cotton variety |
-
2022
- 2022-06-08 CN CN202210642721.7A patent/CN114916431B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN114916431A (en) | 2022-08-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101715754A (en) | Method for raising small brown rice planthopper by utilizing soil-less cultured barley seedling | |
| CN109329029B (en) | Ground cover bamboo water culture cultivation method and cultivation device suitable for laboratory research | |
| CN112544422A (en) | Plant factory planting method for promoting 4-5 ripeness of cereal crops in one year | |
| JPH03195427A (en) | Rapid mass production method for artificial seed potato by tissue culture technique, and use thereof | |
| CN110839522B (en) | Method for controlling growth and development of rice | |
| CN107232061B (en) | A kind of special culture media and tissue culture method of rapid induction bletilla bulb | |
| CN105010142A (en) | Vietnamese Aquilaria agallocha Roxb tissue culture method | |
| CN114916431B (en) | Indoor cultivation method for promoting cotton generation-adding breeding | |
| CN106576788A (en) | Stress resistance breeding method of wheat | |
| CN110036716A (en) | A kind of presoaking and germinating method of corn seed | |
| CN115413578B (en) | Method for cultivating new camellia seedling by utilizing immature hybrid seeds | |
| CN102511397B (en) | Method for inducing populus calli and induction culture medium | |
| CN108934713A (en) | A kind of acer catalpifolium seed seedling-raising method | |
| CN108401907A (en) | A kind of Apples Dwarf Stocks M9T337 tissue cultures volume production method | |
| CN109479721B (en) | A kind of peanut plant regeneration method | |
| Huang et al. | Chinese gooseberry, kiwifruit (Actinidia spp.) | |
| Palee et al. | Influence of plantlet age and different soilless culture on acclimatization of Stemona curtisii Hook. f. | |
| CN113142001B (en) | Method for rapid bell formation during cotton generation-adding breeding | |
| CN111528090A (en) | Strawberry tissue culture seedling hardening matrix for improving hardening quality of strawberry tissue culture seedlings and preparation and use methods and application thereof | |
| CN112293259A (en) | Method for directly inducing adventitious buds of sandalwood tissue culture seedling leaves | |
| CN103053426A (en) | Horseradish plantlet culture method | |
| CN111448985A (en) | Tissue culture method of rosa tenuifolia | |
| CN115281158B (en) | Method for large-scale propagation of phytoseiid chile | |
| CN106577292A (en) | Rapid propagation method for tissue culture of pterocarpus santalinus seedlings | |
| CN118160591A (en) | Method for determining ethephon spraying period of cotton and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |