CN114908014B

CN114908014B - Tea-oil tree endophyte capable of promoting dissolution of ferric phosphate and application of tea-oil tree endophyte

Info

Publication number: CN114908014B
Application number: CN202210575028.2A
Authority: CN
Inventors: 徐婷; 陈永忠; 崔坤鹏; 朱咏华; 彭映赫; 张震; 何之龙; 刘彩霞; 张英
Original assignee: Hunan Academy of Forestry
Current assignee: Hunan Academy of Forestry
Priority date: 2022-05-25
Filing date: 2022-05-25
Publication date: 2023-07-11
Anticipated expiration: 2042-05-25
Also published as: CN114908014A

Abstract

The invention discloses a tea-oil tree endophytic actinomycete for promoting dissolution of ferric phosphate and application thereof, and the strain preservation number of the tea-oil tree endophytic actinomycete is CGMCC No.23481. The tea-oil tree endophyte has excellent phosphorus dissolving capability, can be used for effectively dissolving ferric phosphate, increasing the effective phosphorus content and the iron content in soil and obviously improving the soil, and has various growth promoting functions of fixing nitrogen, producing IAA, siderophores and the like, and can further promote the growth of plants, so that the tea-oil tree endophyte can be used as a soil improver and a biological fertilizer, can be used for dissolving insoluble phosphorus in the soil, increasing the effective phosphorus content in the soil, and obviously promoting the growth of plants on the premise of reducing the using amount of chemical fertilizer, provides new resources for developing microbial fertilizer, soil improver and the like suitable for acid red soil, and has important significance for promoting the growth of plants in the acid soil and improving the yield.

Description

Tea-oil tree endophyte capable of promoting dissolution of ferric phosphate and application of tea-oil tree endophyte

Technical Field

The invention belongs to the technical field of microbial fertilizers and acid red soil crop stress protection, and relates to tea-oil tree endophyte for promoting dissolution of ferric phosphate and application thereof.

Background

The oil tea is a special woody edible oil tree species in China and is a pioneer tree species with ecological benefit and economic benefit, however, the yield of the oil tea is generally not high at present, so that great attention is paid to improving the yield of the oil tea. The phosphorus element is one of a large number of nutrient elements which participate in plant life metabolic activities and important substance composition, and particularly affects the formation and accumulation of oil-tea camellia grease, so that how to increase the content of phosphorus in soil has important significance for promoting the growth of oil-tea camellia and increasing yield and efficiency. At present, most of forest lands for planting oil tea trees are acid red soil, and the defects of large fertilizer application amount, high production cost and the like still exist, and the root cause is that the phosphorus is abundant in the red soil, but the applied phosphorus fertilizer is very easy to be adsorbed and fixed by iron and aluminum oxides to form insoluble iron phosphorus compounds, aluminum phosphorus compounds and other non-effective phosphorus states due to strong oxidation and fixation effects, so that the content of effective phosphorus in the red soil is very low, and the absorption and utilization of plant root systems are not facilitated. Thus, available phosphorus deficiency is one of the major limiting factors in the production of acidic red soil crops.

At present, the improvement and activation of soil indissolvable phosphorus by utilizing microorganisms become a necessary development trend, but the practical application effect of common soil phosphorus-dissolving bacteria is not ideal due to the buffer capacity of soil itself, the decomposition of phosphatase secreted by microorganisms, easy adsorption of soil particles by organic acid and the like, and indissolvable phosphorus in soil is still difficult to be effectively dissolved, which severely restricts the vigorous development of green agriculture, in particular the oil tea industry. In particular, for indissolvable ferric phosphate in soil, the currently reported dissolving capacity of the lysophosphoric bacteria on ferric phosphate is generally weak, so that available phosphorus required by plant growth in soil is still low, and iron elements which are scarce for plants and microorganisms are also very low, and the existence of the defects also restricts the wide application of the lysophosphoric bacteria in improving soil. Therefore, there is an urgent need to obtain a phosphorus-solubilizing bacterium capable of effectively solubilizing the poorly soluble ferric phosphate in the soil, which is of great significance for further increasing the content of phosphorus element in the soil and promoting the growth of plants in the acidic soil.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide the tea-oil tree endophyte for promoting dissolution of ferric phosphate and the application thereof, wherein the tea-oil tree endophyte has excellent phosphorus dissolution capability, can activate various insoluble phosphorus, can effectively dissolve ferric phosphate, increase the content of available phosphorus and iron in soil, can provide sufficient phosphorus source and iron source for plant growth, further promote plant and microorganism growth, can obviously improve the soil, and simultaneously has various growth promotion functions of nitrogen fixation, IAA production, siderophore production and the like, and can further promote plant growth.

In order to solve the technical problems, the invention adopts the following technical scheme:

the tea-oil tree endophytic actinomycetes for promoting dissolution of ferric phosphate are Streptomyces sp.CoH17, and the strain preservation number is CGMCC No.23481.

The tea-oil tree endophyte for promoting dissolution of ferric phosphate is further improved, and the tea-oil tree endophyte is obtained by separating and purifying the root of a tea-oil tree clone 'Xianglin 97'.

The tea-oil tree endophyte for promoting dissolution of ferric phosphate is further improved, and the tea-oil tree endophyte can activate various insoluble phosphors, and particularly can efficiently dissolve ferric phosphate.

As a general technical concept, the invention also provides a soil conditioner, which comprises the oil tea endophyte.

As a general technical concept, the invention also provides application of the soil conditioner in dissolving indissolvable ferric phosphate in soil.

The above application, further improved, comprising the steps of: and mixing the soil conditioner with the soil containing the indissolvable ferric phosphate for culture, and completing the dissolution of the ferric phosphate in the soil.

The application is further improved, and the soil is acid soil; the acid soil is acid red soil.

The invention also provides a biological fertilizer as a general technical concept, wherein the biological fertilizer comprises the oil tea endophyte.

The invention also provides application of the biological fertilizer in promoting plant growth as a general technical concept.

The above application, further improved, comprising the steps of: and (3) pouring the biofertilizer into soil where the plant roots are located, and cultivating the plants.

The above application, further improved, said biofertilizer being applied to the soil in the form of a bacterial suspension; the application amount of the bio-fertilizer bacterial suspension is 30 mL/plant-100 mL/plant; the concentration of the endophyte spores of the oil tea in the bio-fertilizer bacterial suspension is 10 ⁶ Per mL-10 ⁸ individual/mL; the soil is acid soil; the acid soil is acid red soil; the plant is a woody oil crop; the woody oil crop is camellia oleifera.

In the application, the application amount of the bio-fertilizer bacterial suspension is 50 mL/strain; the concentration of the endophyte spores of the oil tea in the bio-fertilizer bacterial suspension is 10 ⁷ And each mL.

Compared with the prior art, the invention has the advantages that:

aiming at the defect that the existing phosphorus-dissolving bacteria are difficult to dissolve indissoluble ferric phosphate, the invention creatively provides a tea-oil tree endophytic actinomycetes for promoting the dissolution of ferric phosphate, which is Streptomyces sp.CoH17 and is obtained by separating and purifying roots of tea-oil tree clone 'Xianglin 97' in a national tea-oil tree germplasm resource collection and conservation (Hunan Changsha) test forest farm. Unlike other conventional endophytes or phosphorus-dissolving bacteria, the tea-oil endophyte provided by the invention has very excellent phosphorus dissolving capability, can activate various insoluble phosphorus, especially can effectively dissolve ferric phosphate, increase the effective phosphorus content and the iron content in soil, can provide sufficient phosphorus source and iron source for plant growth, further promote plant and microorganism growth, and can obviously improve the soil. In practical application, the endophyte strain of the camellia oleifera can be used as a soil conditioner and a biological fertilizer, can dissolve indissolvable phosphorus (especially ferric phosphate) in soil (such as acid red soil), increase the content of available phosphorus and iron elements in the soil, can improve the utilization efficiency of chemical fertilizers and obviously promote the growth of plants (such as woody oil crops such as camellia oleifera) on the premise of reducing the use amount of chemical fertilizers, and has important significance for developing and developing microbial fertilizers, soil conditioners and the like suitable for the acid red soil in south of China and promoting the development and utilization of the acid soil and the growth and yield improvement of plants in the acid soil.

Drawings

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

FIG. 1 is a graph showing colony morphology of actinomycete endophyte strain in oil tea on different types of culture media in example 2 of the present invention.

FIG. 2 is a tree view of the evolution of the endophyte strain of Thermoactinomyces oleracea in example 2 of the present invention.

FIG. 3 is a graph showing the growth of actinomycete endophyte strains in example 3 of the present invention.

FIG. 4 is a graph showing the comparison of the amount of soluble phosphorus in different types by the endophyte strain of Thermoactinomyces oleosa in example 4 of the present invention.

FIG. 5 is a graph showing the effect of the endophyte strain of Thermomyces lanuginosus on the growth of camellia oleifera seedlings in example 6 of the present invention.

Detailed Description

The invention is further described below in connection with the drawings and the specific preferred embodiments, but the scope of protection of the invention is not limited thereby.

In the following examples, unless otherwise specified, the materials and equipment used were commercially available, the processes used were conventional, and the equipment used was conventional.

Example 1:

an endophytic actinomycetes of tea-oil tree for promoting dissolution of ferric phosphate is Streptomyces sp.CoH17, the strain is preserved under the CGMCC No.23481, the preservation date is 2021, 9 months and 26 days, and the strain is preserved in China general microbiological culture Collection center (CGMCC) with the address of Beijing, kogyo, national institute of microbiology, postal code 100101, telephone 010-64807355, fax 01064807288 and E-mail [email protected]. The 16S rRNA gene sequence of the tea-oil tree endophyte is shown as a sequence table SEQ ID NO.1.

The tea-oil camellia endophytic actinomycetes for promoting dissolution of ferric phosphate in the embodiment are plant endophytic actinomycetes obtained by separating and purifying roots of tea-oil camellia clone "Xianglin 97" in a forest farm where a national tea-oil camellia germplasm resource collection and conservation (Hunan Changsha) is located, and the separation, purification and screening processes of tea-oil camellia endophytic actinomycete strains are as follows: the roots of the tea-oil camellia clone 'Xianglin 97' are washed off of sediment, then the plant surface is disinfected, the roots are cut into small sections of about 1 cm after air drying, the small sections are placed in a vitamin B humic acid (HV) endophyte separation culture medium, the culture is carried out at the constant temperature of 30 ℃, and after endophyte is separated out, the endophyte is streaked on an ISP2 solid plate for purification until a pure culture is obtained. And inoculating the pure endophyte culture obtained by separation and purification on a solid plate of an NBRIP phosphorus dissolving culture medium for constant-temperature static culture, observing the generation condition of a phosphorus dissolving ring every day, and further screening to obtain the bacterial strain with good phosphorus dissolving effect.

Example 2:

morphological characteristics and molecular biological characterization of the iron phosphate solubilizing endophyte of camellia oleifera in example 1 above.

1. Morphological characteristics of strains

Morphological characteristics of the strain: the separated and purified endophyte CoH17 of the camellia oleifera is streaked and inoculated into different types of solid culture media, and is cultured at the constant temperature of 30 ℃ for 7 days, and detailed results of the endophyte mycelium and aerial mycelium of the strain are shown in figure 1. Endophytic actinomycetes CoH17 of tea-oil tree can generate endophytic hypha on the tested culture medium, and can generate aerial hypha on other test culture mediums except that the aerial hypha cannot be generated on the glucose asparaginic acid culture medium. Wherein, the strain has better growth and spore production conditions on ISP2, ISP3, ISP5, gao's No.1 and sucrose nitrate 5 solid culture mediums than other culture mediums. And CoH17 produced white or off-white aerial hyphae and white or yellowish-white endophyte in all of the 5 suitable growth media, and the strain did not produce soluble pigments in all of the media tested.

2. Molecular biological identification of strains

Spore suspension of tea-oil tree endophyte CoH17 was prepared and added to ISP2 liquid medium for 5 days at 30℃with shaking at 170 rpm. Genomic DNA of CoH17 was extracted using the Shanghai worker DNA extraction kit and following the protocol described. The 16S rRNA sequence was amplified using the Polymerase Chain Reaction (PCR), with universal primers 27f and 1492r. And (3) sequencing the purified PCR product by a company, wherein the result is shown as a sequence table SEQ ID NO.1. The 16S rRNA sequences of the strains were aligned with the model strain nucleic acid database of the EzBioCloud website, and phylogenetic trees were constructed using the MEGA 5.1 software using the adjacency method, and the results are shown in FIG. 2. As can be seen from fig. 2, the endophyte CoH17 of camellia oleifera is in an independent branch in the phylogenetic tree, and the confidence value of the closest evolutionary branch of CoH17 is only 2, which indicates that the endophyte is not identical with the most similar strain in the evolutionary direction, and the result shows that CoH17 may be a potential new species. It was thus identified as Streptomyces (Streptomyces) and named Streptomyces sp.CoH17.

Example 3:

physiological and biochemical characteristics of the endophyte of tea-oil tree promoting dissolution of iron phosphate and its growth curve in example 1 above.

1. Detection of active substance production by strains

The results of a series of physiological and biochemical assays, such as chitinase production assay, catalase production assay, gelatinase production assay, protease production assay, amylase production assay, cellulase production assay, hemicellulase production assay, casein production assay, melanin production assay, hydrogen sulfide production assay, cyanide production assay, IAA production assay, phosphorus dissolution assay, nitrogen fixation assay, potassium dissolution assay, and siderophore production assay, were performed on tea-oil endophyte CoH17, respectively, and are shown in Table 1. The CoH17 strain is capable of producing a range of enzymes, consisting essentially of: catalase, gelatinase, protease, starch hydrolase, and cellulase; no toxic substances are produced: hydrogen sulfide and hydrogen cyanide; no melanin is produced. Meanwhile, the strain has the capability of producing various growth-promoting related substances such as IAA auxin, phosphorus dissolving, nitrogen fixing, siderophore producing and the like.

TABLE 1 detection of active substance production by CoH17 Strain

Production of active substance test	CoH17
		Chitinase	-
Catalase enzyme	+
		Gelatinase enzyme	+
Protease enzyme	+
		Starch hydrolase	+
Cellulase enzymes	+
		Hemicellulase(s)	-
Casein enzyme	-
		Cyanide compounds	-
Hydrogen sulfide	-
		IAA auxin	+
Melanin pigment	-
		Dissolving phosphorus	+
Nitrogen fixation	+
		Potassium decomposing process	-
Iron carrier	+

Note that: + represents a positive result; -a negative result.

2. Growth conditions of strain and utilization conditions of different carbon sources

Inoculating spore liquid of tea-oil endophyte CoH17 into ISP2 liquid culture medium with pH value of 3-13 according to 1 per mill (v/v) proportion, culturing at 30 ℃ for 7 days, and observing growth conditions of strains in culture media with different pH values; meanwhile, the spore liquid is proportionally added into ISP2 liquid culture medium containing 1 to 10 mass percent of different sodium chloride concentrations, and after the constant temperature culture is carried out for 7 days at 30 ℃, the growth condition of the thalli is observed; spores of CoH17 were inoculated in a liquid medium containing D-fructose, D-galactose, D-mannitol, D-glucose, D-xylose, D-sucrose, L-arabinose, L-inositol and maltose as the only carbon sources in a ratio of 1%o (v/v), and incubated at a constant temperature of 30℃for 7 days to observe the growth conditions. The results are shown in Table 2, and actinomycetes CoH17 can grow well in the pH range of 5-12, and the highest concentration of sodium chloride tolerance is 9%, further explaining that the strain belongs to the high salt tolerance strain. As a result of inoculating CoH17 to a basal medium containing different carbon sources in a ratio of 1% and culturing at a constant temperature, it was found that the strain did not grow in a medium having xylose, inositol and sucrose as the sole carbon sources, and that the strain grew well in other types of media such as glucose, galactose and the like as the sole carbon sources. Therefore, from the aspect of the growth characteristics of the strain, most of carbon sources can be utilized by the strain, the strain has very high salt tolerance, the pH value range suitable for growth is very wide, the adaptability to the growth environment is high, and the strain is particularly suitable for being used as a microbial fertilizer in the planting of plants in the southern woodland.

TABLE 2 growth conditions and carbon utilization of CoH17 Strain

Detection index	CoH17
		D-glucose	+
D-galactose	+
		D-mannitol	+
L-arabinose	±
		D-fructose	±
D-xylose	-
		L-inositol	-
D-sucrose	-
		Maltose	±
pH value of	5-12
		Salinity tolerance	9％

Note that: + represents a positive result; + -indicates a weak positive result; -a negative result.

3. Growth curve of strain

Configuration of ISP2 liquidThe body culture medium is subpackaged and subjected to high-temperature sterilization treatment, and spore liquid of the tea-oil endophyte CoH17 is prepared and added into ISP2 liquid culture medium, so that the final concentration of the spore liquid in the culture medium is 1 multiplied by 10 ⁴ And each mL. Culturing at 30deg.C and 170rpm in shaker for 8 days, sampling three bottles each day, filtering, collecting thallus, oven drying at 45deg.C for 48 hr, weighing dry thallus weight, and making into figure 3. As shown in FIG. 3, the endophyte CoH17 of the camellia oleifera directly enters the logarithmic phase at the 1 st day of inoculation, the growth speed is high, the maximum value of the growth amount of the thallus is reached at the 3 rd day, and the growth period starts to enter the decay period at the 5 th day. Therefore, when the ISP2 liquid medium is used for shaking culture, the CoH17 can reach the maximum amount of the grown bacteria on the 3 rd day of growth.

Example 4:

investigating the dissolving capacity of tea-oil tree endophyte CoH17 strain on different types of insoluble phosphorus

The endophyte CoH17 of the camellia oleifera in the example 1 is respectively inoculated into a liquid phosphorus-dissolving culture medium containing 5g/L calcium phosphate, 5g/L ferric phosphate and 5g/L aluminum phosphate, shake culture is carried out at 30 ℃ and 170rpm for 7 days, centrifugation is carried out at 4000rpm for 20min after the culture is finished, a molybdenum-antimony colorimetric method is adopted for obtaining supernatant, and the effective phosphorus content in the fermentation broth is measured at the wavelength of 700 nm. As a result, as shown in FIG. 4, coH17 was able to effectively dissolve various types of poorly soluble phosphorus, in which the amount of dissolution of calcium phosphate was 313.35 mg/L at the highest, followed by 78.28mg/L for ferric phosphate and 47.49mg/L for aluminum phosphate.

The most abundant insoluble phosphorus in acid soil is iron phosphate and aluminum phosphate, which are normally difficult to dissolve by common phosphorus-dissolving bacteria. The results of phosphorus solubilizing ability comparison of CoH17 with other phosphorus solubilizing bacteria, phosphorus solubilizing actinomycetes and phosphorus solubilizing fungi are shown in table 3. For example, none of the lysophosphoric Bacillus licheniformis A3, brevibacillus borstelensis SH, 168, streptomyces thermophilus J57, streptomyces thermonitrificans NTU-88 can dissolve ferric phosphate. The amounts of dissolved phosphorus bacteria of Streptomyces sp.CoT10, amycolatopsis sp.M4 and Aspergillus fumigatus NTU-132 which are dissolved in iron phosphate are lower than those of actinomycetes CoH17. Therefore, the tea-oil camellia endophyte CoH17 obviously has the capability of dissolving various insoluble phosphorus, particularly high-efficiency ferric phosphate, so that the strain is particularly suitable for improving the problem of lack of available phosphorus in the southern acidic red soil rich in ferric phosphate.

TABLE 3 comparison of the dissolving capacities of different phosphorus-dissolving bacteria for different inorganic phosphorus

Note that: ND indicates no detection.

Example 5:

a soil conditioner, in particular to an endophyte actinomycetes of camellia oleifera.

The application of the soil conditioner in the implementation in dissolving indissolvable ferric phosphate in soil, specifically, the improvement and activation of acid soil by utilizing the tea-oil tree endophyte actinomycetes in the embodiment 1, the improvement of the effective phosphorus content and the iron element content of the soil, comprises the following steps:

inoculating the endophyte CoH17 of Camellia oleifera in example 1 onto soybean mannitol agar Medium (MS) plate, growing for 7-10 days, and making spore into 10-concentration ⁷ individual/mL spore suspension. Mixing the prepared spore suspension with soil for planting camellia oleifera seedlings, specifically, pouring the spore suspension into the soil where the roots of the camellia oleifera seedlings are located, and applying 50mL of spore liquid to each camellia oleifera seedling. All test seedlings were only normally watered during the test period and no other fertilization treatments were performed. And culturing the camellia oleifera seedlings for 30 days. Sterile water was used as a control instead of spore suspension.

And respectively collecting the camellia oleifera potting soil treated by the endophyte CoH17 of the camellia oleifera and the potting soil of the camellia oleifera of the control group which is not treated by the CoH17, and determining the element content of the soil. The soil organic matter is measured by adopting a dichromate wet burning method and a visible spectrophotometry; the determination of total phosphorus in soil adopts an ignition method; the available phosphorus was extracted using 100ml of 0.5 molar sodium bicarbonate leach and assayed for available phosphorus content using molybdenum antimony anti-colorimetry, with three replicates per test. The results are shown in Table 4, and the acid soil is treated by the tea-oil tree endophyte CoH17, so that the pH value of the soil can be improved, and the content of organic matters in the soil can be increased. Wherein, the contents of organic matters, total phosphorus and available phosphorus in the soil are respectively increased by 97.59 percent, 55.26 percent and 16.98 percent.

The method further shows that the tea-oil endophyte CoH17 can obviously improve acid red soil, efficiently activate indissoluble phosphorus in soil, increase the effective phosphorus content in the soil, provide sufficient phosphorus source for plant growth, further promote plant growth, and be used for preparing soil conditioner applicable to southern acid red soil areas. Therefore, the tea-oil camellia endophyte can be used as an improver for improving and activating acid soil, so as to promote the growth of plants.

TABLE 4 influence of CoH17 Strain on element content in soil

Soil element content	Control group	Treatment group
			pH value of	4.69±0.05	5.03±0.06*
Organic matter (g/kg)	86.13±0.02	170.18±2.25***
			Total phosphorus (g/kg)	0.76±0.02	1.18±0.03**
Available phosphorus (mg/kg)	115.80±0.13	135.46±5.07

Example 6:

a biological fertilizer, in particular to a bacterial suspension of tea-oil tree endophyte cultivated by tea-oil tree endophyte.

The application of the biofertilizer in the implementation in promoting plant growth, in particular to the application of spore liquid of tea-oil tree endophyte to promote plant growth in soil, comprising the following steps:

inoculating the endophyte CoH17 of Camellia oleifera in example 1 onto soybean mannitol agar Medium (MS) plate, growing for 7-10 days, and making spore into 10-concentration ⁷ individual/mL spore suspension. And (3) carrying out root irrigation treatment on the camellia oleifera seedlings by using the prepared spore liquid, and applying 50mL of spore liquid to each camellia oleifera seedling. All test seedlings were only normally watered during the test period and no other fertilization treatments were performed. And after the camellia oleifera seedlings grow for a period of time, determining related indexes of the camellia oleifera seedlings, and measuring growth indexes such as spring tip length, ground diameter, fresh weight, dry weight and the like of the camellia oleifera seedlings, wherein the result is shown in figure 5. As shown in fig. 5, the application of the endophyte CoH17 of the camellia oleifera can significantly promote the growth of camellia oleifera seedlings, wherein the spring tip length of the camellia oleifera seedlings treated by the CoH17 is significantly higher than that of the control group for 3 months continuously, and the average growth is 21.60%; the ground diameter of the oil tea seedling treatment group is increased by 39.97% compared with that of the control group. Meanwhile, compared with a control group, the fresh weight and the dry weight of the camellia oleifera seedlings in the CoH17 treatment group are also obviously increased, especially the fresh weight of root systems is increased by more than 2 times, which proves that after the tea-oil endophyte CoH17 is inoculated into the roots of the camellia oleifera, the tea-oil endophyte CoH17 can quickly form a symbiotic relationship with the camellia oleifera, and further promote the growth of the camellia oleifera seedlings, and the analysis is probably due to the obvious increase of the content of available phosphorus and iron elements for promoting the growth of plants in soil under the action of the tea-oil endophyte CoH17, thereby being based on plantsThe growth condition can also indicate that the tea-oil camellia endophyte CoH17 can activate various insoluble phosphorus, in particular can effectively dissolve ferric phosphate so as to increase the effective phosphorus content and the iron content in soil, thereby providing sufficient phosphorus source and iron source for the growth of plants. Therefore, the tea-oil camellia endophyte disclosed by the invention can be used as a biological fertilizer for promoting the growth of plants.

From the above results, unlike other conventional actinomycetes, the tea-oil endophyte of the invention has excellent phosphorus dissolving capability, can activate various insoluble phosphorus, particularly can dissolve ferric phosphate with high efficiency, increase the effective phosphorus content and the iron content in soil, can provide sufficient phosphorus source and iron source for plant growth, further promote plant and microorganism growth, and can obviously improve the soil. In practical application, the endophyte strain of the camellia oleifera can be used as a soil conditioner and a biological fertilizer, can dissolve indissolvable phosphorus (especially ferric phosphate) in soil (such as acid red soil), increase the content of available phosphorus and iron elements in the soil, can improve the utilization efficiency of chemical fertilizers and obviously promote the growth of plants (such as woody oil crops such as camellia oleifera) on the premise of reducing the use amount of chemical fertilizers, and has important significance for developing and developing microbial fertilizers, soil conditioners and the like suitable for the acid red soil in south of China and promoting the development and utilization of the acid soil and the growth and yield improvement of plants in the acid soil.

The above examples are only preferred embodiments of the present invention, and the scope of the present invention is not limited to the above examples. All technical schemes belonging to the concept of the invention belong to the protection scope of the invention. It should be noted that modifications and adaptations to the present invention may occur to one skilled in the art without departing from the principles of the present invention and are intended to be within the scope of the present invention.

Sequence listing

<110> Proc of forestry science in Hunan province

<120> tea-oil tree endophyte promoting dissolution of ferric phosphate and application thereof

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Claims

1. The tea-oil camellia endophytic actinomycetes for promoting dissolution of ferric phosphate is characterized in that the tea-oil camellia endophytic actinomycetes are Streptomyces sp.CoH17, and the strain preservation number is CGMCC No.23481.

2. The tea-oil tree endophyte for promoting the dissolution of ferric phosphate according to claim 1, wherein the tea-oil tree endophyte is obtained by separating and purifying the tea-oil tree endophyte from the root of a clone "Xianglin 97".

3. A soil conditioner comprising the endophyte of camellia oleifera as claimed in claim 1 or 2.

4. Use of the soil conditioner of claim 3 for dissolving poorly soluble ferric phosphate in soil.

5. The use according to claim 4, characterized in that it comprises the following steps: mixing the soil conditioner with soil containing indissolvable ferric phosphate for culture to complete the dissolution of the ferric phosphate in the soil; the soil is acid soil; the acid soil is acid red soil.

6. A biofertilizer characterized in that it comprises the endophyte of camellia oleifera as claimed in claim 1 or 2.

7. Use of the biofertilizer of claim 6 for promoting plant growth.

8. The use according to claim 7, characterized by the steps of: and (3) pouring the biofertilizer into soil where the plant roots are located, and cultivating the plants.

9. The use according to claim 8, wherein the biofertilizer is applied to the soil in the form of a bacterial suspension; the application amount of the bio-fertilizer bacterial suspension is 30 mL/plant-100 mL/plant; the concentration of the endophyte spores of the oil tea in the bio-fertilizer bacterial suspension is 10 ⁶ Per mL-10 ⁸ individual/mL; the soil is acid soil; the acid soil is acid red soil; the plant is a woody oil crop; the woody oil crop is camellia oleifera.

10. Use according to claim 9, characterized in that the bio-fertilizer bacterial suspension is applied in an amount of 50 mL/strain; the concentration of the endophyte spores of the oil tea in the bio-fertilizer bacterial suspension is 10 ⁷ And each mL.