CN114885944B - Active organic acid Flukon disinfectant and application method thereof - Google Patents
Active organic acid Flukon disinfectant and application method thereof Download PDFInfo
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- CN114885944B CN114885944B CN202210577218.8A CN202210577218A CN114885944B CN 114885944 B CN114885944 B CN 114885944B CN 202210577218 A CN202210577218 A CN 202210577218A CN 114885944 B CN114885944 B CN 114885944B
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- 239000000645 desinfectant Substances 0.000 title claims abstract description 105
- 150000007524 organic acids Chemical class 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- VTIIJXUACCWYHX-UHFFFAOYSA-L disodium;carboxylatooxy carbonate Chemical compound [Na+].[Na+].[O-]C(=O)OOC([O-])=O VTIIJXUACCWYHX-UHFFFAOYSA-L 0.000 claims description 14
- 229940045872 sodium percarbonate Drugs 0.000 claims description 14
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 claims description 12
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 claims description 12
- 239000000174 gluconic acid Substances 0.000 claims description 12
- 235000012208 gluconic acid Nutrition 0.000 claims description 12
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 10
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 10
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 10
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 10
- 239000001632 sodium acetate Substances 0.000 claims description 10
- 235000017281 sodium acetate Nutrition 0.000 claims description 10
- 235000005979 Citrus limon Nutrition 0.000 claims description 9
- 244000131522 Citrus pyriformis Species 0.000 claims description 9
- 240000000111 Saccharum officinarum Species 0.000 claims description 9
- 235000007201 Saccharum officinarum Nutrition 0.000 claims description 9
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 9
- 240000006394 Sorghum bicolor Species 0.000 claims description 9
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 9
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 7
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 36
- 241000700605 Viruses Species 0.000 abstract description 30
- 230000001954 sterilising effect Effects 0.000 abstract description 30
- 241000894006 Bacteria Species 0.000 abstract description 18
- 230000001580 bacterial effect Effects 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 11
- 239000004094 surface-active agent Substances 0.000 abstract description 9
- 238000004140 cleaning Methods 0.000 abstract description 7
- 108090000623 proteins and genes Proteins 0.000 abstract description 7
- 239000000872 buffer Substances 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 6
- 230000035699 permeability Effects 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 239000007800 oxidant agent Substances 0.000 abstract description 4
- 230000001590 oxidative effect Effects 0.000 abstract description 4
- 150000002978 peroxides Chemical class 0.000 abstract description 4
- 241000233866 Fungi Species 0.000 abstract description 3
- 239000007853 buffer solution Substances 0.000 abstract description 3
- 210000000170 cell membrane Anatomy 0.000 abstract description 3
- 239000001301 oxygen Substances 0.000 abstract description 3
- 229910052760 oxygen Inorganic materials 0.000 abstract description 3
- 231100000419 toxicity Toxicity 0.000 abstract description 3
- 230000001988 toxicity Effects 0.000 abstract description 3
- 102000018697 Membrane Proteins Human genes 0.000 abstract description 2
- 108010052285 Membrane Proteins Proteins 0.000 abstract description 2
- 241000204031 Mycoplasma Species 0.000 abstract description 2
- 241001052560 Thallis Species 0.000 abstract description 2
- 230000003042 antagnostic effect Effects 0.000 abstract description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 2
- 230000008859 change Effects 0.000 abstract description 2
- 238000010494 dissociation reaction Methods 0.000 abstract description 2
- 230000005593 dissociations Effects 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 20
- 239000007787 solid Substances 0.000 description 13
- 239000000047 product Substances 0.000 description 12
- 239000007788 liquid Substances 0.000 description 11
- 239000000243 solution Substances 0.000 description 11
- 239000000843 powder Substances 0.000 description 10
- 239000000725 suspension Substances 0.000 description 10
- 238000005303 weighing Methods 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 4
- 241000709661 Enterovirus Species 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 239000002274 desiccant Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 239000011259 mixed solution Substances 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 239000012091 fetal bovine serum Substances 0.000 description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 3
- 230000005180 public health Effects 0.000 description 3
- 150000003242 quaternary ammonium salts Chemical class 0.000 description 3
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 241000222122 Candida albicans Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 2
- -1 alkalis Chemical class 0.000 description 2
- 229940095731 candida albicans Drugs 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 229910017053 inorganic salt Inorganic materials 0.000 description 2
- 150000004694 iodide salts Chemical class 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 108010077805 Bacterial Proteins Proteins 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000003876 biosurfactant Substances 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 150000004678 hydrides Chemical class 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 150000004965 peroxy acids Chemical class 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- KKCBUQHMOMHUOY-UHFFFAOYSA-N sodium oxide Chemical compound [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 1
- 229910001948 sodium oxide Inorganic materials 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- MWNQXXOSWHCCOZ-UHFFFAOYSA-L sodium;oxido carbonate Chemical compound [Na+].[O-]OC([O-])=O MWNQXXOSWHCCOZ-UHFFFAOYSA-L 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to an active organic acid Flukon disinfectant and a use method thereof, wherein the disinfectant comprises the following components in parts by mass: 20-30 parts of active organic acid, 20-30 parts of oxidant, 1-2 parts of surfactant and 30-50 parts of buffer component. The sterilization effect of the active organic acid disinfectant of the invention is that the high concentration of H + Can denature and hydrolyze thallus protein, and has low concentration of H + The dissociation degree of the amphoteric substances of the bacterial body surface proteins can be changed; peroxide destroys active genes of thalli, and a buffer system releases OH-free radicals and active oxygen free radicals to destroy RNA and DNA of viruses and bacteria; the surfactant can reduce the surface tension, emulsify to remove greasy dirt, play a cleaning role, and can change the permeability of cell membranes of bacteria so as to denature the proteins of the bacteria; has excellent antagonistic effect on bacteria, mold, mycoplasma and fungi, and has the advantages of ultralow toxicity, no corrosiveness, no environmental residue, broad spectrum, high efficiency and the like.
Description
Technical Field
The invention relates to a novel active organic acid Flukon disinfectant which is widely applied to a plurality of industries such as medical treatment, sanitation, cultivation, food and the like.
Background
The increased awareness of public household disinfection has also prompted a great need for sterile products. The improvement of people's safety and sanitation consciousness puts higher and higher demands on the quantity and quality of disinfectant in many aspects such as pets, public health safety, cultivation, food, public health and the like.
Currently, commonly used disinfectants are peroxides, alkalis, iodides and iodides, chlorine-containing compounds, quaternary ammonium salts, and the like. However, the traditional disinfectant has the defects of low efficiency, high cost, secondary pollution and the like, can not meet the current social demands, and the market improvement of the disinfectant is urgent. At present, disinfectant enterprises in China are fewer, the disinfectant yield is far from meeting the social demands, most of disinfectant products in China are relatively simple in production process, market consumer products mainly use middle and low ends, and the disinfectant is a disinfectant which is short in broad spectrum, efficient, stable in performance, easy to dissolve in water, quick in action, long in duration, good in permeability, not easy to be influenced by various physical and chemical factors, harmless to people, livestock and articles, economical and convenient to use.
Patents on disinfectants have also been reported, for example: CN103004875A discloses a kitchen sterilizing disinfectant and a preparation method thereof, CN113367155A discloses a potassium hydrogen persulfate compound disinfectant and application thereof, and CN114208828A discloses a disinfectant containing a biosurfactant and a preparation method and application thereof. However, the existing disinfectant has the defects of high toxicity, easiness in causing secondary pollution to the environment, long sterilization time, low effective components of the product, incapability of being used for emergency use and the like, and influences the popularization of the product.
Disclosure of Invention
Aiming at the defects of the prior art, especially the production process of the disinfectant product in the prior art is relatively simple, the market consumer product takes the middle and low end as the main stream, and the disinfectant which has low toxicity, does not cause secondary pollution to the environment, is easy to dissolve in water, has rapid action and is not easily influenced by various physical and chemical factors is lacking. The invention aims to provide the Flukon disinfectant which is safe and free of toxic and side effects, environment-friendly, pollution-free, rapid in sterilization, safe in use, small in influence of physical and chemical factors on the food-grade raw material, good in permeability and low in toxicity, and the product is finally degraded into inorganic salt.
The technical scheme of the invention is as follows:
an active organic acid Flukon disinfectant comprises the following components in parts by mass:
20-30 parts of active organic acid, 20-30 parts of oxidant, 1-2 parts of surfactant and 30-50 parts of buffer component.
According to the invention, the active organic acid Flukon disinfectant is a solid powder, preferably, the active organic acid Flukon disinfectant further comprises 5-15 parts of drying agent. The desiccant can protect the solid components from moisture.
According to the invention, preferably, the active organic acid Flukon disinfectant also comprises 1-2 parts of lemon essence, so that the smell of the disinfectant is pleasant and comfortable.
According to the invention, preferably, the active organic acid Flukon disinfectant further comprises 0.5 to 1 part of an indicator. When the disinfectant agent is oxidizing, it dissolves in water to develop a color, and when no longer develops a color to indicate that the disinfectant is non-oxidizing, the disinfectant fails.
According to the invention, preferably, the active organic acid is gluconic acid, and the gluconic acid has the advantages of low cost and good sterilization effect.
According to the invention, preferably, the oxidizing agent is sodium percarbonate.
According to the present invention, preferably, the surfactant is sodium dodecyl sulfate.
According to the present invention, preferably, the buffer component is sodium acetate and potassium dihydrogen phosphate.
According to the present invention, preferably, the desiccant is anhydrous sodium sulfate.
According to the invention, the indicator is preferably sorghum red or sugar cane red.
According to the invention, preferably, the active organic acid Flukon disinfectant comprises the following components in parts by mass:
20-30 parts of gluconic acid, 20-30 parts of sodium percarbonate, 1-2 parts of sodium dodecyl sulfate, 0.5-1 part of sorghum red or sugarcane red, 15-25 parts of sodium acetate, 15-25 parts of monopotassium phosphate, 1-2 parts of lemon essence and 5-15 parts of anhydrous sodium sulfate.
The Flukon disinfectant disclosed by the invention is a stable synergistic system, has green and environment-friendly components, contains a surfactant, active organic acid and peroxide, and greatly improves the disinfection effect of the disinfectant. The gluconic acid is used as a main sterilization component; sodium percarbonate is an oxidizing agent, which oxidizes gluconic acid to polygluconic acid; the sodium dodecyl sulfate is used as a surfactant, so that the effective components of the disinfectant can smoothly destroy RNA and DNA of viruses and bacteria through bacterial cell walls and virus protein shells, and the effect of permanent killing is achieved; sorghum red or sugarcane red is used as an indicator, the indicator shows red when the disinfectant reagent has oxidability, and the indicator shows no oxidability when the indicator shows no red any more, so that the disinfectant fails; sodium acetate and potassium dihydrogen phosphate are used as buffer components, so that the disinfectant is prepared in various water environments, and the pH is still in the applicable range; lemon essence, so that the smell of the disinfectant is pleasant and comfortable; the anhydrous sodium sulfate drying agent protects the solid component and prevents the solid component from absorbing moisture.
The sterilization effect of the active organic acid disinfectant is as follows: high concentration of H + (hydride) can denature and hydrolyze the bacterial protein, and has low concentration of H + The dissociation degree of the amphoteric substances of the bacterial body surface proteins can be changed; peroxide destroys active genes of thalli, and a buffer system releases OH - (hydroxy)Free radicals and reactive oxygen radicals destroy RNA, DNA of viruses and bacteria; the surfactant can reduce the surface tension, emulsify to remove greasy dirt, play a cleaning role, and can change the permeability of cell membranes of bacteria so as to denature the proteins of the bacteria; has excellent antagonistic effect on bacteria, mold, mycoplasma and fungi, and has the advantages of ultralow toxicity, no corrosiveness, no environmental residue, broad spectrum, high efficiency and the like.
According to the invention, the method for using the active organic acid Flukon disinfectant comprises the following steps:
uniformly mixing disinfectant and water, soaking a target object, evaporating, and spraying by a sprayer; preferably, the mass ratio of disinfectant to water is 1: (50-200), further preferably 1: (80-120), most preferably 1:100.
According to the invention, the disinfectant has the following application ranges: (1) disinfection of various pathogens carried by animals; (2) disinfection of a wide range of sudden public health events; (3) Sterilizing stations, ships and various transportation vehicles of a common poplar station of a conventional high-density crowd; (4) disinfection of aquaculture; (5) disinfection of hospitals and various medical instruments; (6) disinfection of drinking water equipment and drinking water; (7) air disinfection.
When the disinfectant is used for disinfecting public places and pet environments, the disinfectant is uniformly mixed with water, then the target objects are soaked, evaporated and sprayed by a sprayer for use, so that common pathogens carried by the public places and the pets can be effectively killed. The active organic acid disinfectant is a powdery solid, is dissolved into pink liquid when meeting water, and is invalid when the liquid color is eliminated.
The invention has the following effective results:
1. the disinfectant disclosed by the invention is stable in performance, easy to dissolve in water, not easy to be influenced by various physical and chemical factors, is a solid disinfectant, has high concentration of active ingredients, and can be used for emergency use; the buffer component is added to ensure that the disinfectant can be prepared with various water qualities, and the requirement on the water quality is low. Ensure the preparation of disinfectant in various water environments, and the pH value is still in the applicable range.
2. The disinfectant has rapid action, can kill viruses for 1min, and glutaraldehyde is more than 10min, and quaternary ammonium salt is more than 30 min.
3. The disinfectant disclosed by the invention is convenient to use, compared with quaternary ammonium salt disinfectants, the sodium percarbonate composite powder is not interfered by organic matters, compared with aldehydes, the sodium percarbonate composite powder is not affected by temperature, and the sterilization time is shortened; the spray application is more convenient, and the product itself has red color as indicator, and the red color disappears.
4. The disinfectant food-grade raw material is safe and has no toxic or side effect; the product is finally degraded into inorganic salt, is environment-friendly and pollution-free, and belongs to environment-friendly disinfectants.
Drawings
FIG. 1 shows the results of the E.coli killing test in test example 1.
FIG. 2 shows the result of the test example 1 for killing Staphylococcus aureus.
FIG. 3 shows the result of the test for killing Bacillus subtilis in test example 1.
FIG. 4 shows the results of the Candida albicans killing test in test example 1.
Detailed description of the preferred embodiments
The present invention will be described in detail below. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
1. Preparation of novel active organic acid Flukon disinfectant:
weighing the following components in parts by mass: 20 parts of gluconic acid, 20 parts of sodium percarbonate, 1 part of sodium dodecyl sulfate, 1 part of sorghum red or sugarcane red, 18 parts of sodium acetate, 18 parts of monopotassium phosphate, 1 part of lemon essence and 5 parts of anhydrous sodium sulfate, and uniformly mixing the above components to prepare the solid powder active organic acid disinfectant.
Weighing 10 parts by mass of water in a container, slowly pouring 1 part by mass of the prepared solid powder active organic acid disinfectant into the container, uniformly stirring, and then adding water to dilute the mixture until the mass ratio of the cleaning disinfectant to the water is 1:100, thereby preparing the novel active organic acid Flukon disinfectant solution.
2. Laboratory sterilization test:
1) Preparation of E.coli bacterial liquid
Viable count is carried out on the prepared bacterial liquid, and then the bacterial liquid is diluted into a sterile 10 containing solution with 0.9 percent of sterile sodium chloride 6 ～10 7 cfu/mL of test bacterial liquid.
2) Sucking 0.5mL of the bacterial liquid into 4.5mL of the active organic acid disinfectant solution prepared in the embodiment, and uniformly mixing; and (5) standing.
3) 0.5mL of the mixed bacterial liquid was sucked up and added to 4.5mL of a neutralization solution (0.5% sodium thiosulfate solution) and mixed well for neutralization for 10 minutes. Taking out 0.1mL of the mixed solution of the fungus and the medicine, putting the mixed solution into a culture dish added with a nutrient agar culture medium, and uniformly coating the mixed solution; the dishes were incubated at 37℃for 24 hours, and the number of colonies was counted to calculate the sterilization rate. The results are shown in Table 1.
Sucking 0.5mL of the bacterial drug mixed solution prepared in 2) when the standing time is 30 minutes, repeating 3), counting the colony number and calculating the sterilization rate. The results are shown in Table 1.
3. Laboratory comparative sterilization test:
1) The steps of the laboratory sterilization test of 2 were repeated, wherein sterile water was used instead of the active organic acid disinfectant used as a positive control group. The results are shown in Table 1.
2) The steps of the laboratory sterilization test of 2 were repeated except that no bacteria liquid and no active organic acid disinfectant were added, and the test group was used as a negative control. The results are shown in Table 1.
Example 2
1. Preparation of solid powder active organic acid Flukon disinfectant:
weighing the following components in parts by mass: 25 parts of gluconic acid, 25 parts of sodium percarbonate, 1 part of sodium dodecyl sulfate, 1 part of sorghum red or sugarcane red, 18 parts of sodium acetate, 18 parts of monopotassium phosphate, 1 part of lemon essence and 5 parts of anhydrous sodium sulfate, and uniformly mixing the above components to prepare the novel active organic acid Flukon disinfectant.
Weighing 10 parts by mass of water in a container, slowly pouring 1 part by mass of the prepared novel active organic acid Flukon disinfectant into the container, uniformly stirring, and then adding water to dilute the mixture until the mass ratio of the cleaning disinfectant to the water is 1:100, thereby preparing the novel active organic acid Flukon disinfectant.
2. Laboratory sterilization tests were performed in the same manner as in example 1, and the results are shown in Table 1.
3. Laboratory comparative sterilization tests were performed in the same manner as in example 1: the results are shown in Table 1
Example 3
1. Preparation of solid powder active organic acid Flukon disinfectant:
weighing the following components in parts by mass: 20 parts of gluconic acid, 20 parts of sodium percarbonate, 1 part of sodium dodecyl sulfate, 1 part of sorghum red or sugarcane red, 20 parts of sodium acetate, 20 parts of monopotassium phosphate, 1 part of lemon essence and 5 parts of anhydrous sodium sulfate, and uniformly mixing the above components to prepare the novel active organic acid Flukon disinfectant.
Weighing 10 parts by mass of water in a container, slowly pouring 1 part by mass of the prepared novel active organic acid Flukon disinfectant into the container, uniformly stirring, and then adding water to dilute the solution until the mass ratio of the cleaning disinfectant to the water is 1:100, thereby preparing the Flukon disinfectant solution.
2. Laboratory sterilization tests were performed in the same manner as in example 1, and the results are shown in Table 1.
3. Laboratory comparative sterilization tests were performed in the same manner as in example 1: the results are shown in Table 1.
Example 4
1. Preparation of novel active organic acid Flukon disinfectant:
weighing the following components in parts by mass: 25 parts of gluconic acid, 25 parts of sodium percarbonate, 1 part of sodium dodecyl sulfate, 1 part of sorghum red or sugarcane red, 20 parts of sodium acetate, 20 parts of monopotassium phosphate, 1 part of lemon essence and 5 parts of anhydrous sodium sulfate, and uniformly mixing the above components to prepare the novel active organic acid Flukon disinfectant.
Weighing 10 parts by mass of water in a container, slowly pouring 1 part by mass of the prepared novel active organic acid Flukon disinfectant into the container, uniformly stirring, and then adding water to dilute the mixture until the mass ratio of the cleaning disinfectant to the water is 1:100, thereby preparing the novel active organic acid Flukon disinfectant.
2. Laboratory sterilization tests were performed in the same manner as in example 1, and the results are shown in Table 1.
3. Laboratory comparative sterilization tests were performed in the same manner as in example 1: the results are shown in Table 1.
TABLE 1
As can be seen from table 1: example 2 achieves a full bactericidal effect at a concentration of 1:100 for each example.
In various embodiments of the invention, laboratory sterilization tests were performed using E.coli. The invention is characterized in that sodium percarbonate oxidizes gluconic acid to be peroxy acid, so that the sterilization capability is greatly enhanced. Sodium dodecyl sulfate is used as a surfactant, the surfactant can be adsorbed on the surface of bacteria, the permeability of cell membranes of bacteria is changed, enzymes, coenzyme and intermediate metabolites in the bacteria are escaped, and the respiration and glycolysis processes of the bacteria are blocked, so that the bacteria proteins are denatured, and the sterilization effect is presented; sodium acetate and potassium dihydrogen phosphate are used as buffer components, and a buffer system is used for releasing OH- (hydroxyl) free radicals and active oxygen free radicals to destroy RNA and DNA of viruses and bacteria, so as to achieve the effect of permanent killing.
Test example 1
The results of the experiments of the high-efficiency solid powder active organic acid Flukon disinfectant of example 2 on the sterilization of different bacteria are shown in fig. 1-4. Fig. 1 is a result of an escherichia coli killing experiment, fig. 2 is a result of a staphylococcus aureus killing experiment, fig. 3 is a result of a bacillus subtilis killing experiment, and fig. 4 is a result of a candida albicans killing experiment; in FIGS. 1-4, a. Shows the distribution of four bacterial colonies under a microscope without the addition of the active organic acid Flukon disinfectant, b. Shows the distribution of four bacterial colonies under a microscope with the addition of the active organic acid Flukon disinfectant, c. Shows the distribution of four bacterial colonies in a culture dish without the addition of the active organic acid Flukon disinfectant, d. Shows the distribution of four bacterial colonies in a culture dish with the addition of the active organic acid Flukon disinfectant.
As can be seen from FIGS. 1-4, the organic acid Flukon disinfectant of the present invention has good bactericidal effect against different bacteria.
Test example 2
The product of the invention is tested for killing viruses for 1min
1. The purpose of the experiment is as follows: the disinfectant has the advantages of rapid action and killing of various viruses within 1 min.
2. The test steps are as follows:
2.1 cell culture:
after resuscitating the frozen RD cells, placing the cells in a carbon dioxide incubator for culture, observing the cell state every day, periodically changing the liquid to keep the good cell state, pouring out the old culture solution in the flask after the cells in the flask grow to be full of a single layer, adding a proper amount of cell digestive solution Trypsin (the proper liquid level covers the cells), immediately pouring out the digestive solution when the cells are rounded and scattered, adding a proper amount of fresh MEM culture medium containing 10% fetal bovine serum, blowing and mixing until the cells fall off from the wall of the flask, preparing a cell suspension, and then placing the cell suspension in a 37 ℃ 5% carbon dioxide incubator for culture.
2.2 Virus amplification
(1) The inoculated virus is taken out from the refrigerator at 4 ℃ and the preserved enterovirus virus seeds (preserved in the refrigerator at-80 ℃ for a long time) are inoculated after the enterovirus seeds are melted. Firstly, the monolayer cells which grow in an adherence way need to be full of about 70% -80%, old culture medium is discarded, a proper amount of MEM culture medium containing 2% fetal bovine serum is replaced, 100 mu L of virus suspension is needed to be put into a large cell culture bottle, then the cell culture bottle is cultured in a 5% carbon dioxide incubator at 37 ℃, and cytopathy is observed day by day.
(2) When the pathological changes of the harvested viruses are observed under a microscope to be moderate, a centrifuge tube is used for collecting suspension in a culture bottle, the suspension is centrifuged for 5min under the condition of 5000r/min, or the suspension is centrifuged for 10min at 3000r/min, and then the supernatant is taken and split-packed into 50mL centrifuge tubes and placed in a refrigerator at 4 ℃ or at minus 20 ℃ for standby. Before use, the virus titer is measured, and if the virus titer is not used for a long time, the virus titer is reduced, and the virus needs to be re-amplified at the moment so as not to influence the experimental effect.
2.3 solid powder active organic acid Flukon disinfectant inactivated enterovirus
A suspension of virus of a certain titer was prepared and the virus was diluted to the desired titer with 10% phosphate buffer (pH 7.2). The same volume of virus suspension is taken and added into two culture dishes respectively, then the culture dishes are placed on a magnetic stirrer to be slightly stirred, and if the depth of the virus suspension in the culture dishes is less than 2mm, the culture dishes can be not stirred. An appropriate amount of the novel active organic acid Flukon disinfectant disclosed by the invention is added into one part of a culture dish, a sterilization experiment is carried out for 1 minute, after 1 minute is finished, a sample is wrapped by tinfoil, and the coated sample is placed in a refrigerator at 4 ℃ to prepare for detecting the titer. And (3) adding no disinfectant into the other culture dish, staying for 1 minute, taking a sample after 1 minute is finished, wrapping with tinfoil, and placing in a refrigerator at 4 ℃ to prepare for detecting titer.
2.4 detection of enterovirus titres
(1) The cell concentration was first calculated using a cell counting plate, then the cells were diluted to a concentration of 1X 105 cells/mL, 100. Mu.L was added to a 96-well plate using a row gun, 7000 cells per well, and 1:100 antibiotics were added.
(2) 100 mu L of the virus suspension added with the disinfectant and 100 mu L of the virus suspension without the disinfectant are respectively taken by an EP tube, 900 mu L of MEM culture medium containing 2% of fetal bovine serum is respectively added and is marked as-1, after the mixture is fully mixed, 100 mu L of the two virus suspensions are respectively taken and added into the next EP tube, and the viruses are serially diluted by the same method.
(3) Labeling was performed on 96-well plates corresponding to the EP tube according to dilution.
(4) In 96-well plates, 50 μl of virus dilution was added per well. Then, the 96-well plate containing the virus dilution was sealed with a sealing film, placed in an incubator containing 5% carbon dioxide at 37℃and the titer was calculated after 3-4 days. Finally, the normal RD cell number and the lesion RD cell number in the two virus dilutions are counted under a microscope.
3. Test results: the number of pathological cells in the virus diluent added with the disinfectant is zero, and almost all cells in the virus diluent without the disinfectant are pathological cells. The test effect proves that the active organic acid Flukon disinfectant has rapid action, can kill viruses within 1min, and has the killing rate of 100%.
Test example 3
Comparative test of the product of example 2 with or without sodium oxide percarbonate
1. The purpose of the experiment is as follows: as can be seen from Table 1, example 2 achieves a complete bactericidal effect, and a comparative experiment with example 2, in which sodium percarbonate oxide is present, demonstrates that the principle of the present invention is that the oxide acts upon oxidation of the organic acid.
2. Test step one:
1) Preparation of novel active organic acid Flukon disinfectant:
weighing the following components in parts by mass: 25 parts of gluconic acid, 0 part of sodium percarbonate, 1 part of sodium dodecyl sulfate, 1 part of sorghum red or sugarcane red, 18 parts of sodium acetate, 18 parts of monopotassium phosphate, 1 part of lemon essence and 5 parts of anhydrous sodium sulfate, and uniformly mixing the above components to prepare the novel active organic acid Flukon disinfectant.
Weighing 10 parts by mass of water in a container, slowly pouring 1 part by mass of the prepared solid powder active organic acid disinfectant into the container, uniformly stirring, and then adding water to dilute the mixture until the mass ratio of the cleaning disinfectant to the water is 1:100, thereby preparing the novel active organic acid Flukon disinfectant solution.
2) Laboratory sterilization tests were performed in the same manner as in example 1, and the results are shown in Table 2.
3) Laboratory comparative sterilization tests were performed in the same manner as in example 1: the results are shown in Table 2.
3. Test results: the sterilization rate is 12% when the sodium percarbonate which is an oxidation component is absent, and the sterilization capability of the organic acid can be increased only when the oxide oxidizes the organic acid.
TABLE 2
Claims (2)
1. The active organic acid Flukon disinfectant is characterized by comprising the following components in parts by mass:
20-30 parts of gluconic acid, 20-30 parts of sodium percarbonate, 1-2 parts of sodium dodecyl sulfate, 0.5-1 part of sorghum red or sugarcane red, 15-25 parts of sodium acetate, 15-25 parts of monopotassium phosphate, 1-2 parts of lemon essence and 5-15 parts of anhydrous sodium sulfate.
2. A method of using the active organic acid Flukon disinfectant of claim 1 comprising the steps of:
uniformly mixing disinfectant and water, and then soaking or evaporating a target object, or spraying the mixture of the disinfectant and the water by using a sprayer;
the mass ratio of disinfectant to water is 1: (50-200).
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| US9701931B2 (en) * | 2013-09-30 | 2017-07-11 | Chemlink Laboratories, Llc | Environmentally preferred antimicrobial compositions |
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| JPH11148098A (en) * | 1997-11-14 | 1999-06-02 | Kao Corp | Solid sterilizing detergent for hard body |
| JP2001354997A (en) * | 2000-06-12 | 2001-12-25 | Matsumura Sekiyu Kenkyusho:Kk | Gluconic acid detergent |
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