CN114874879B

CN114874879B - Support frame with clamping function and biological reaction device

Info

Publication number: CN114874879B
Application number: CN202210808073.8A
Authority: CN
Inventors: 许俊; 李飙; 于搏洋; 胡福林; 易帅; 丁晨; 唐辉; 徐江运; 徐龙渤; 徐茏林
Original assignee: Zhejiang JYSS Bio Engineering Co Ltd
Current assignee: Zhejiang JYSS Bio Engineering Co Ltd
Priority date: 2022-07-11
Filing date: 2022-07-11
Publication date: 2022-09-16
Anticipated expiration: 2042-07-11
Also published as: CN114874879A

Abstract

The invention relates to a support frame with a clamping function and a biological reaction device. The support frame is arranged between the biological reaction bag and the culture tank, and comprises a support part and a clamping part, wherein the support part is provided with a support part which is raised compared with the inner wall of the culture tank, and the clamping part is connected with the support part; the clamping piece can clamp the welding edge of the biological reaction bag, or the clamping piece can be matched with the supporting piece to clamp the welding edge of the biological reaction bag; the biological reaction bag is supported by the supporting part, so that the wrinkles of the biological reaction bag can be reduced or even eliminated, on one hand, the wrinkles are prevented from influencing the scouring of the culture solution on the inner wall of the biological reaction bag, the dissolved oxygen effect in the culture process is increased, on the other hand, the wrinkles are prevented from being scoured by the cell culture solution, so that the plastic deformation of the wrinkles is caused, and white spots easy to leak are formed; simultaneously, through pressing from both sides the support frame and tightly being fixed in the welding limit, can fully strut the part of biological reaction bag near the welding limit to the fold elimination effect of maximize support frame.

Description

Support frame with clamping function and biological reaction device

Technical Field

The invention relates to the technical field related to a biological reaction device, in particular to a support frame with a clamping function and the biological reaction device.

Background

The bioreactor bag is a bag-packaging device prepared from a membrane material, is widely applied to laboratory research and commercial production operation, and can be particularly used for production operation of ready-to-use cell culture, purification, fluid treatment and the like. The biological reaction bag filled with liquid is filled into the tank body of the culture tank, and particularly the biological reaction bag with large capacity inevitably generates wrinkles on the membrane material on the bottom surface of the bag body, particularly the membrane material at the shape mutation position of the bag body, thereby bringing negative influence to the biological reaction process.

Disclosure of Invention

In view of the above, it is desirable to provide a support frame with a clamping function and a bioreactor.

The invention provides a support frame with a clamping function, which is arranged between a biological reaction bag and a culture tank and comprises a support part and a clamping part, wherein the support part is provided with a supporting part protruding than the inner wall of the culture tank; the clamping member can clamp the welding edge of the biological reaction bag, or the clamping member can be matched with the supporting member to clamp the welding edge of the biological reaction bag.

The support frame supports the biological reaction bag relative to the culture tank through the support part raised relative to the inner wall of the culture tank, so that the wrinkles of the biological reaction bag are reduced or even eliminated, on one hand, the wrinkles are prevented from influencing the washing of the culture solution on the inner wall of the biological reaction bag, the dissolved oxygen effect in the culture process is increased, on the other hand, the wrinkles at the wrinkles are prevented from being subjected to plastic deformation and forming white spots which are easy to leak due to the fact that the cell culture solution washes the wrinkles of the biological reaction bag for a long time; simultaneously, through pressing from both sides the support frame and tightly being fixed in the welding limit, can fully strut the part that is close to the welding limit of biological reaction bag to the fold elimination effect of maximize support frame.

In one embodiment, the clamping member comprises a clamping portion, the clamping portion and the supporting portion can move close to each other to clamp the welding edge of the bioreactor bag and move away from each other to release the welding edge of the bioreactor bag; the clamping part and the supporting part can clamp the welding edge of the biological reaction bag in a line contact or surface contact mode.

It can be understood that the clamping part and the supporting part can be switched between a clamping state and a loosening state according to actual use requirements, and the universality of the support frame is improved. In addition, the welding edge of the biological reaction bag is clamped in a line contact or surface contact mode, the clamping area is large, the clamping stability is good, and the welding edge is not easy to be damaged.

In one embodiment, in the clamped state, there is a gap between the clamping portion and the support portion.

It can be understood that, under the clamping state, clamping part and welding limit overlap portion roughly are banana shape, and the great part of welding limit middle part size can take place to curl, and the setting in space can be with curled partial holding in the space to avoid curled portion to influence final tight thickness of clamp, guarantee that the centre gripping is firm between clamping part and the supporting part.

In one embodiment, the void is crescent-shaped in a radial cross-section of the cage.

It can be understood that the crescent-shaped gap can accommodate the banana-shaped welding edge while avoiding the occurrence of the situation that the gap is too large to affect the normal clamping.

In one embodiment, the clamping portion has a bottom edge and two side edges at a side relatively close to the supporting portion, and the bottom edge and the two side edges are capable of clamping the welding edge of the bioreactor bag in line contact with the supporting portion.

It can be understood that the bottom edge and the two side edges of the clamping part can clamp the welding edge of the biological reaction bag in a line contact manner with the supporting part, so that a gap is formed between the clamping part and the supporting part to increase the clamping firmness of the clamping part and the supporting part, and the contact length of the clamping part and the supporting part is longer when the clamping part and the welding edge are clamped to further increase the clamping firmness.

In one embodiment, the clamping member further comprises an extending portion, the extending portion is connected to the clamping portion, and the clamping portion and the extending portion are arranged in a V shape.

It can be understood that the extension part can support the cylindrical bag body, so as to abut and support the biological reaction bag, and further reduce wrinkles on the biological reaction bag; in addition, the extension part can also block the biological reaction bag, and the biological reaction bag is prevented from touching the connecting structure between the clamping part and the supporting part, so that the clamping effect is influenced.

In one embodiment, the clamping portion is provided with a through hole.

It can be understood that the through hole can increase the deformability of the clamping portion, and the clamping portion is prevented from being plastically deformed or broken due to excessive deformation.

In one embodiment, the clamping piece still includes the installation department, the installation department is connected the clamping part and the supporting part, and is a plurality of the through-hole is rectangular shape through-hole, rectangular shape through-hole is by being close to relatively one side of installation department extends to the one side of keeping away from relatively the installation department, and is a plurality of rectangular shape through-hole is parallel to each other.

In one embodiment, the support frame further comprises a buckle, the buckle is arranged on the clamping piece or the supporting piece, and the clamping portion and the supporting portion are movably connected through the buckle to realize switching between a clamping state and a releasing state.

It can be understood that the support frame is movably connected through the buckle, the operation is simple, the switching between the clamping state and the loosening state can be realized according to the requirement, and the universality of the support frame is improved.

The invention provides a biological reaction device, which comprises a culture tank, a biological reaction bag and the support frame, wherein the culture tank comprises a cylindrical part and a circular table part, and the cylindrical part is connected to the large-diameter end of the circular table part and is positioned at the top of the circular table part; the biological reaction bag correspondingly comprises a cylindrical bag body and a circular truncated cone-shaped bag body, the cylindrical bag body and the large-diameter end of the circular truncated cone-shaped bag body are welded to form the welding edge, the cylindrical bag body is contained in the cylindrical part of the culture tank, and the circular truncated cone-shaped bag body is contained in the circular truncated part of the culture tank.

It can be understood that, the fold of biological reaction bag mainly appears near the hookup location of cylindric bag body and the round platform form bag body usually, and be near welding limit promptly, through pressing from both sides the support frame and tightly be fixed in welding limit, can fully strut the cylindric bag body of biological reaction bag and the part that the round platform form bag body is close to welding limit to the fold elimination effect of maximize support frame.

Drawings

FIG. 1 is a schematic view showing the structure of a bioreactor bag according to an embodiment;

FIG. 2 is a schematic sectional view showing the structure of the culture tank and the support frame in one embodiment;

FIG. 3 is a schematic perspective view of a support frame according to an embodiment;

FIG. 4 is a schematic view of a partial structure of a bioreactor in one embodiment;

FIG. 5 is a schematic longitudinal cross-sectional view of one embodiment of a brace in a clamped condition;

FIG. 6 is a top view of the support of FIG. 3 in a clamped condition;

FIG. 7 is a schematic cross-sectional view of the support frame A-A of FIG. 6;

FIG. 8 is a schematic top view showing a partial structure of a bioreactor in one embodiment;

FIG. 9 is a schematic diagram of the construction of a clamp according to one embodiment;

FIG. 10 is a schematic longitudinal cross-sectional view of another embodiment of a stand in an unfastened condition;

FIG. 11 is a schematic longitudinal cross-sectional view of a support stand according to another embodiment;

FIG. 12 is a schematic perspective view of a stand in another embodiment;

FIG. 13 is a perspective view of the clamping member of FIG. 12;

fig. 14 is a schematic perspective view of a support frame in another embodiment.

100. A support frame; 10. a support member; 11. a support portion; 111. a threaded member; 20. a clamping member; 21. a clamping portion; 211. a bottom edge; 212. a side edge; 213. a through hole; 214. a light hole; 22. an extension portion; 23. an installation part; 30. a void; 40. a threaded fastener; 50. an anti-slip member; 60. buckling; 200. a culture tank; 210. a cylindrical portion; 220. a circular table portion; 300. a biological reaction bag; 310. a cylindrical bag body; 320. a circular truncated cone-shaped bag body; 330. and (6) welding edges.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

It will be understood that when an element is referred to as being "connected to" another element, it can be directly connected to the other element or intervening elements may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or intervening components may also be present. When an element is referred to as being "secured to" another element, it can be directly secured to the other element or intervening elements may also be present.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "or/and" includes any and all combinations of one or more of the associated listed items.

The bioreactor device comprises a bioreactor and a bioreactor bag 300, wherein the bioreactor comprises a shaking table (not shown) and a culture tank 200, the bioreactor bag 300 is used for containing a culture solution, the bioreactor bag 300 is contained in the culture tank 200, and the shaking table can drive the culture tank 200 to move, so as to drive the culture solution to flow. However, when the bioreactor bag 300 filled with liquid is loaded into the culture tank 200, particularly, when the bioreactor bag 300 having a large capacity is loaded, wrinkles are inevitably generated on the membrane material of the bottom surface of the bioreactor bag 300, which may adversely affect the bioreactor process.

Based on this, please refer to fig. 1, fig. 2, fig. 3 and fig. 4, the present invention first provides a support frame 100 with a clamping function, the support frame 100 is disposed between a bioreactor bag 300 and a culture tank 200, the support frame 100 includes a support member 10 and a clamping member 20, the support member 10 is provided with a support portion 11 protruding from an inner wall of the culture tank 200, the clamping member 20 is connected to the support member 10; the clamping member 20 can clamp the welding rim 330 of the bioreactor bag 300, or the clamping member 20 can clamp the welding rim 330 of the bioreactor bag 300 in cooperation with the support member 10.

Since the supporting portion 11 of the supporting member 10 is protruded with respect to the inner wall of the culture tank 200, after the bioreactor bag 300 is placed on the inner wall of the culture tank 200 and the supporting member 10, the supporting member 10 can press the bioreactor bag 300 corresponding to the supporting portion 11 toward the inner direction thereof through the supporting portion 11, i.e., the bioreactor bag 300 is supported with respect to the culture tank 200, thereby achieving the effect of reducing or even eliminating wrinkles of the bioreactor bag 300.

The folds of the biological reaction bag 300 are reduced, so that the effect of the cell culture solution on washing the inner wall of the biological reaction bag 300 can be avoided from being influenced by the folds, and the dissolved oxygen effect in the culture process is increased; on the other hand, the phenomenon that plastic deformation occurs at the folds and 'white spots' are formed, which are easy to leak, due to the fact that the cell culture fluid washes the folds of the biological reaction bag 300 for a long time can be avoided.

It should be noted that, in general, the wrinkles of the bioreactor bag 300 mainly appear near the abrupt change position of the shape of the bag, i.e. near the welding edge 330, and by clamping and fixing the support frame 100 to the welding edge 330, the part of the bioreactor bag 300 close to the welding edge 330 can be sufficiently spread, thereby maximizing the wrinkle removing effect of the support frame 100.

In some embodiments, culture tank 200 is a culture tank in a torrent bioreactor.

When the torrent bioreactor is in operation, the culture tank 200 is driven by the shaking table and eccentrically moves according to a certain eccentric distance and a certain rotating speed, and the cell culture solution in the culture tank 200 generates turbulent flow similar to tidal effect.

The turbulent flow generated by the cell culture fluid continuously washes the inner wall of the biological reaction bag 300, so that the oxygen bubbles in the cell culture fluid are broken or divided into smaller bubbles by impact, thereby achieving the effect of increasing the dissolved oxygen amount and the dissolved oxygen efficiency in the cell culture fluid.

It can be understood that if there are wrinkles on the inner wall of the bioreactor bag 300, on the one hand, the effect of the cell culture fluid washing the inner wall of the bioreactor bag 300 is affected, and thus the dissolved oxygen effect is affected; on the other hand, the cell culture fluid washes the wrinkles of the bioreactor bag 300 for a long time, which causes stress accumulation at the wrinkles, eventually generates "white spots" of plastic deformation and fatigue marks accompanying the "shell type", and the wrinkles generating the "white spots" are prone to leak, thereby causing a large loss.

The traditional paddle type bioreactor has the working principle that a paddle positioned in the middle of the bioreactor rotates to drive cell culture solution to flow so as to increase the dissolved oxygen amount and dissolved oxygen efficiency of the cell culture solution, and the dissolved oxygen of the bioreactor does not depend on the inner wall of a biological reaction bag 300, so that the influence of the wrinkles on the inner wall of the biological reaction bag 300 on the work of the biological reaction bag can be almost ignored, and technicians in the field have no motivation to arrange the support frame on the basis of the paddle type bioreactor.

In some embodiments, clamp 20 is capable of individually clamping welded edges 330 of bioreactor bag 300, such as: the clamping member 20 is provided with a clamping member which can clamp the welding edge 330 of the biological reaction bag 300 by means of self elasticity, torsion spring and the like; the clamping member 20 is provided with a screw member and clamps the welding edge 330 of the bio-reaction bag 300 by the screw member.

Of course, the clamping member 20 can also clamp the welding edge 330 of the bioreactor bag 300 by other conventional fixing means, which is not specifically limited herein.

Referring to fig. 3, 4, 5 and 6, in some embodiments, the clamping member 20 includes a clamping portion 21, the clamping portion 21 and the supporting portion 11 can be moved toward each other to clamp the welding edge 330 of the bioreactor bag 300, and can be moved away from each other to release the welding edge 330 of the bioreactor bag 300; the clamping part 21 and the supporting part 11 can clamp the welding edge 330 of the bioreactor bag 300 in a line contact or a surface contact.

Here, the welding edge 330 of the bio-reaction bag 300 is clamped in a line contact or a surface contact, which means that the welding edge 330 can be clamped when the clamping part 21 and the supporting part 11 are brought close to each other. It should be noted that when the clamping portion 21 and the supporting portion 11 approach each other to the limit position, a line/surface contact state may be adopted, and a non-contact state may be adopted. For example: in the present embodiment, the clamping portion 21 and the supporting portion 11 can be in line/surface contact when the clamping portion 21 and the supporting portion 11 approach each other to the limit position, but in other embodiments, the clamping portion 21 and the supporting portion 11 may not be in contact when the clamping portion 21 and the supporting portion 11 approach each other to the limit position, and the clamping portion 21 and the supporting portion 11 can clamp the welding edge 330 as long as the distance between the clamping portion 21 and the supporting portion 11 in the limit position is smaller than the thickness of the welding edge 330.

Referring to fig. 4, 7 and 8, in some embodiments, in the clamping state, a gap 30 is formed between the clamping portion 21 and the supporting portion 11; in fig. 8, the overlapping portion (as indicated by a line p in fig. 8) of the clamping portion 21 and the welding edge 330 is the welding edge 330 of the portion clamped between the clamping portion 21 and the supporting portion 11, the welding edge 330 is substantially banana-shaped, and the area of the central portion is larger than the areas of the two ends; in the process that the clamping portion 21 and the supporting portion 11 approach each other to clamp the welding edge 330, the central portion of the welding edge 330 is easily curled, the thickness of the welding edge 330 of the curled portion increases, which affects the final clamping thickness, and the gap 30 can accommodate the curled portion, thereby ensuring the clamping firmness between the clamping portion 21 and the supporting portion 11.

In the present embodiment, the gap 30 is formed by the difference in radian between the clamping portion 21 and the support portion 11; of course, the gap 30 may be formed by other methods, such as forming a groove on the clamping portion 21 or the supporting portion 11, and the application is not limited in this respect.

Referring to fig. 3, 4 and 9, in some embodiments, the side of the holding portion 21 relatively close to the supporting portion 11 has a bottom side 211 and two side sides 212, and the bottom side 211 and the two side sides 212 can both clamp the welding edge 330 of the bioreactor bag 300 in line contact with the supporting portion 11.

It can be understood that, under the condition of the same clamping force, the smaller the stressed area is, the larger the pressure intensity of the stressed position is, and the better the clamping effect is; in this embodiment, the bottom 211 and the two sides 212 of the clamping portion 21 can clamp the welding edge 330 of the bioreactor bag 300 in line contact with the supporting portion 11, so that on one hand, the gap 30 is formed between the clamping portion 21 and the supporting portion 11 to increase the clamping firmness of the two, and on the other hand, the pressure at the clamping position when the clamping portion 330 is clamped by the two is increased to further increase the clamping firmness.

Referring to fig. 7 and 8, in some embodiments, the gap 30 is crescent-shaped in a radial cross section of the support frame 100; the area change trend of the radial section of the crescent-shaped gap 30 in the radial direction is the same as the area change trend of the banana-shaped welding edge 330 in the radial direction, so that the phenomenon that the normal clamping is affected due to the overlarge gap 30 can be avoided while the curled part welding edge 330 is accommodated.

Referring to fig. 3, 4, 5 and 6, in some embodiments, the clamping member 20 further includes an extending portion 22, the extending portion 22 is connected to the clamping portion 21, and the clamping portion 21 and the extending portion 22 are disposed in a V-shape.

The extension part 22 can also abut against the bioreactor bag 300, and in this embodiment, the extension part 22 can support the cylindrical bag body 310 so as to abut against and support the bioreactor bag 300, thereby further reducing wrinkles on the bioreactor bag 300; in addition, the extension portion 22 can block the bioreactor bag 300 from touching the connection structure (e.g., torsion spring structure, snap structure, or screw connection structure) between the clamping portion 21 and the supporting portion 11, thereby affecting the clamping effect.

Referring to fig. 3, in some embodiments, the clamping portion 21 has a through hole 213; so as to increase the deformation capacity of the clamping part 21 and avoid the situation that the clamping part 21 is plastically deformed or broken due to overlarge deformation.

Referring to fig. 3 and 5, in some embodiments, the clamping member 20 further includes a mounting portion 23, the mounting portion 23 connects the clamping portion 21 and the supporting portion 11, the through holes 213 are all elongated through holes, the elongated through holes extend from a side relatively close to the mounting portion 23 to a side relatively far from the mounting portion 23, and the elongated through holes are parallel to each other.

Of course, the through hole 213 may be an opening having another shape, or may be a groove opened on one side or both sides of the clamping portion 21, as long as the deformability of the clamping portion 21 can be increased.

Referring to fig. 10, in some embodiments, the clamping portion 21 is provided with a skid-proof member 50 on a side thereof relatively close to the supporting portion 11; and/or one side of the supporting part 11 close to the clamping part 21 is provided with an anti-slip piece 50.

The anti-slip member 50 can increase the friction between the clamping portion 21 and/or the supporting portion 11 and the welding edge 330 in the clamping state, thereby achieving the effect of increasing the clamping firmness between the clamping portion 21 and the supporting portion 11.

In some embodiments, cleats 50 are rubber or other material having a relatively high coefficient of friction.

In some embodiments, the anti-slip element 50 is a thread on the side surface of the clamping portion 21 and/or the supporting portion 11 adjacent to each other.

Referring to fig. 1 to 11, in some embodiments, the clamping member 20 is fixedly connected to the supporting member 10, at least the clamping portion 21 of the clamping member 20 has elasticity, and the clamping portion 21 and the supporting portion 11 can approach each other to elastically clamp the welding edge 330 of the bioreactor bag 300. The loss of the welding edge 330 of the bioreactor bag 300 can be reduced while the clamping force is ensured through elastic clamping, so that the service life of the bioreactor bag 300 is prolonged as much as possible. Of course, the clamping portion 21 may also have no elasticity, and the clamping portion 21 and the supporting portion 11 are pressed to contact and separate from each other by a common fixing connection structure such as a threaded structure and a clamping structure.

Specifically, in some embodiments, the clamping member 20 and the supporting member 10 are fixedly connected by bonding, welding, or the like, and the clamping member 20 and the supporting member 10 may also be integrally formed.

In some embodiments, not shown, the supporting frame 100 includes a torsion spring, and the clamping portion 21 and the supporting portion 11 are rotatably connected by the torsion spring to realize the switching between the clamping state and the releasing state.

In some embodiments, as shown in fig. 14, the supporting bracket 100 further includes a buckle 60, the buckle 60 is disposed on the clamping member 20 or the supporting member 10, and the clamping portion 21 and the supporting portion 11 are movably connected through the buckle 60 to switch between the clamping state and the releasing state.

It can be understood that the buckle 60 is movably connected, the operation is simple, and the switching between the clamping state and the releasing state can be realized as required, so that the versatility of the support frame 100 is increased.

Specifically, the clamping portion 21 is provided with a protrusion, and the supporting portion 11 is provided with a groove at a corresponding position, and the protrusion can clamp the welding edge 330 of the bioreactor bag 300 in the groove, so as to detachably connect the support frame 100 to the welding edge 330 of the bioreactor bag 300. It is understood that the latch 60 may be a plurality of protrusions and a plurality of grooves, or may be one protrusion with an elongated shape and one groove with an elongated shape.

As shown in fig. 12 and 13, in some embodiments, the supporting frame 100 further includes a threaded fastener 40, the clamping member 20 is connected to the supporting member 10 through the threaded fastener 40, and the clamping portion 21 and the supporting portion 11 are switched between the clamping state and the releasing state by screwing and unscrewing the threaded fastener 40.

In this embodiment, the screw 111 is fixedly disposed on the supporting portion 11, the clamping member 20 is provided with a light hole 214 through which the screw 111 can penetrate, and after the screw 111 penetrates through the light hole 214, the position of the screw 40 can be adjusted along the axial direction of the screw 111 by the threaded connection between the screw 40 and the screw 111, that is, the screw 40 is screwed or unscrewed, so as to switch between the clamping state and the loosening state.

The clamping part 21 and the supporting part 11 are connected through the threaded part 111 and the threaded fastener 40, on one hand, the clamping or loosening degree can be linearly adjusted, the adjustable degree is high, and the general applicability is strong; on the other hand, the clamping force provided by the threaded connection is relatively large, so that a high degree of clamping firmness between the support portion 11 and the clamping portion 21 can be ensured.

Referring to fig. 1, 2 and 4, a second aspect of the present invention provides a bioreactor, comprising a culture tank 200, a bioreactor bag 300 and the above-mentioned support frame 100 with clamping function, wherein the culture tank 200 comprises a cylindrical part 210 and a circular table part 220, the cylindrical part 210 is connected to the large diameter end of the circular table part 220 and is located at the top of the circular table part 220; the bioreactor bag 300 comprises a cylindrical bag body 310 and a circular truncated cone-shaped bag body 320 correspondingly, the cylindrical bag body 310 and the large-diameter end of the circular truncated cone-shaped bag body 320 are welded to form a welding edge 330, the cylindrical bag body 310 is accommodated in the cylindrical part 210 of the culture tank 200, the circular truncated cone-shaped bag body 320 is accommodated in the circular truncated cone part 220 of the culture tank 200, and the support frame 100 is arranged between the culture tank 200 and the bioreactor bag 300; the support frame 100 can clamp the welding edge 330 of the bioreactor bag 300 by the clamping member 20, or the clamping member 20 can clamp the welding edge 330 of the bioreactor bag 300 by cooperating with the support member 10.

It should be noted that, in general, wrinkles of the bioreactor bag 300 mainly appear near the connection position of the cylindrical bag body 310 and the truncated cone-shaped bag body 320, that is, near the welding edge 330, and by clamping and fixing the support frame 100 to the welding edge 330, the portions of the cylindrical bag body 310 and the truncated cone-shaped bag body 320 of the bioreactor bag 300 close to the welding edge 330 can be sufficiently spread, thereby maximizing the wrinkle removing effect of the support frame 100.

It is understood that one or more support members 10 may be provided, and two or more support members 10 may be uniformly arranged around the joint of the cylindrical part 210 and the circular truncated part 220 in the culture tank 200, or may be arranged according to the actual folding condition.

The features of the above-described embodiments may be arbitrarily combined, and for the sake of brevity, all possible combinations of the features in the above-described embodiments are not described, but should be construed as being within the scope of the present disclosure as long as there is no contradiction between the combinations of the features.

It should be understood by those skilled in the art that the above embodiments are only for illustrating the present invention and are not to be used as a limitation of the present invention, and that suitable changes and modifications of the above embodiments are within the scope of the claimed invention as long as they are within the spirit and scope of the present invention.

Claims

1. A support frame with a clamping function is characterized in that the support frame (100) is arranged between a bioreactor bag (300) and a culture tank (200), the support frame (100) comprises a support member (10) and a clamping member (20), a support part (11) which is raised compared with the inner wall of the culture tank (200) is arranged on the support member (10), and the clamping member (20) is connected to the support member (10);

the clamping member (20) is capable of clamping the welding edge (330) of the bioreactor bag (300), or the clamping member (20) is capable of clamping the welding edge (330) of the bioreactor bag (300) in cooperation with the support member (10).

2. The support rack with clamping function as claimed in claim 1, wherein said clamping member (20) comprises a clamping portion (21), said clamping portion (21) and said supporting portion (11) being capable of approaching each other to clamp said welding edge (330) of said bioreactor bag (300) and moving away from each other to release said welding edge (330) of said bioreactor bag (300);

the clamping part (21) and the supporting part (11) can clamp the welding edge (330) of the biological reaction bag (300) in a line contact or surface contact mode.

3. A support with clamping function according to claim 2, characterized in that in the clamped state there is a gap (30) between the clamping portion (21) and the support portion (11).

4. A support with clamping function according to claim 3, characterized in that said interspace (30) is crescent-shaped in radial section of the support (100).

5. The support with clamping function as claimed in claim 2, wherein the clamping portion (21) has a bottom edge (211) and two side edges (212) on a side relatively close to the support portion (11), and the bottom edge (211) and the two side edges (212) are capable of clamping the welding edge (330) of the bioreactor bag (300) in line contact with the support portion (11).

6. A support bracket with clamping function according to claim 2, characterized in that the clamping member (20) further comprises an extension part (22), the extension part (22) is connected to the clamping part (21), and the clamping part (21) and the extension part (22) are arranged in a V-shape.

7. A support with clamping function as claimed in claim 2, wherein said clamping portion (21) is provided with a through hole (213).

8. A support frame with clamping function as claimed in claim 7, wherein said clamping member (20) further comprises a mounting portion (23), said mounting portion (23) connects said clamping portion (21) and said supporting portion (11), said through holes (213) are all elongated through holes, said elongated through holes extend from a side relatively close to said mounting portion (23) to a side relatively far from said mounting portion (23), and said elongated through holes are parallel to each other.

9. The support with clamping function as claimed in any one of claims 2 to 8, wherein said support (100) further comprises a buckle (60), said buckle (60) is disposed on said clamping member (20) or said supporting member (10), and said clamping portion (21) and said supporting portion (11) are movably connected through said buckle (60) to realize the switching between the clamping state and the releasing state.

10. A bioreactor apparatus, comprising a culture tank (200), a bioreactor bag (300) and the support frame (100) with clamping function of any one of claims 1 to 9, wherein the culture tank (200) comprises a cylindrical part (210) and a circular table part (220), the cylindrical part (210) is connected to the large diameter end of the circular table part (220) and is positioned at the top of the circular table part (220); the biological reaction bag (300) correspondingly comprises a cylindrical bag body (310) and a truncated cone-shaped bag body (320), the cylindrical bag body (310) and the large-diameter end of the truncated cone-shaped bag body (320) are welded to form the welding edge (330), the cylindrical bag body (310) is accommodated in the cylindrical part (210) of the culture tank (200), and the truncated cone-shaped bag body (320) is accommodated in the truncated cone part (220) of the culture tank (200).