CN114873837B - Method and device for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae - Google Patents
Method and device for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F1/30—Treatment of water, waste water, or sewage by irradiation
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2866—Particular arrangements for anaerobic reactors
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
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- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/10—Photocatalysts
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- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention provides a method and a device for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae, wherein the method comprises the steps of sending sewage into a short-range denitrification-anaerobic ammoxidation reaction zone for treatment; algae are enriched in the short-range denitrification-anaerobic ammoxidation reaction zone and photocatalytic materials are added; adding acetic acid into the sewage as a carbon source; in the treatment process, visible light is used for illuminating the reaction area, and the quantity of algae is controlled by adjusting illumination parameters, so that the dissolved oxygen concentration of the reaction area is kept to be 0.9-1.5mg/L, and ammonia oxidizing bacteria are generated in situ. According to the invention, by adding the photocatalytic material into the PD/A reaction zone, the in-situ enrichment of algae in the bioreactor is promoted, and meanwhile, the means of regulating the illumination condition is adopted, so that a micro-aerobic environment is created and maintained, the in-situ growth of AOB flora is promoted, a bacteria-algae symbiotic PD/A-PN/A system is formed, the nitrogen and phosphorus removal of the system is enhanced, and the synchronous and efficient removal of nitrogen and phosphorus is realized.
Description
Technical Field
The invention belongs to the technical field of water treatment, and particularly relates to a method and a device for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae.
Background
Along with the large discharge of nitrogen and phosphorus elements in urban sewage, the urban sewage becomes a main factor of water eutrophication, so that the dephosphorization and denitrification of the sewage become important problems in sewage treatment. Anaerobic ammoxidation refers to the use of NO under anoxic conditions 2 - N as electron acceptor, NH 4 + Direct conversion of N to N 2 . The anaerobic ammonia oxidation process (ANAMMOX) can realize the effective treatment of the high ammonia nitrogen wastewater under the condition of no need of additional carbon source and aeration. However, in actual wastewater, NH is often present 4 + high-N content and NO 2 - -the source of N is unstable, thereby subjecting ANAMMOX to NO 2 - Insufficient limitation of N matrix, resulting in reduced denitrification efficiency. In recent years, short-range denitrification (PD) has been used as a relatively stable NO-capturing agent 2 - The novel pathway of N, which is often coupled to ANAMMOX to formThe short-cut denitrification-anaerobic ammonia oxidation (PD/A) system further strengthens the denitrification efficiency, saves about 40% of carbon source requirements compared with the traditional nitrification-denitrification process, and has the effects of saving aeration energy consumption and reducing sludge yield. However, the separate PD/A process cannot effectively and synchronously remove phosphorus when sewage is treated.
The traditional biological phosphorus removal device utilizes excessive phosphorus absorption of phosphorus accumulating bacteria by anaerobic/aerobic technology to achieve the aim of biological phosphorus removal of sewage. By the end of the 20 th century, it was found that a denitrification phosphorus accumulating bacterium can enrich phosphorus and nitrogen in an anaerobic/anoxic alternate operation environment, thereby creating conditions for synchronous removal of nitrogen and phosphorus. On the basis, people further screen denitrifying phosphorus removal bacteria which take nitrite as an electron acceptor, so that short-range denitrification phosphorus removal is realized. In the prior art, an SBR reactor and an anaerobic ammonia oxidation reactor are commonly used, the SBR reactor firstly performs anoxic denitrification to remove excessive nitrite in the upper period, then performs anaerobic phosphorus release, performs aerobic phosphorus absorption and partial short-range nitrification, and the effluent enters the anaerobic ammonia oxidation reactor together with sludge fermentation liquor, so that ammonia nitrogen and nitrite are removed through autotrophic denitrification of anaerobic ammonia oxidation bacteria, and the denitrification and dephosphorization of domestic sewage are realized.
In the prior art, denitrification and dephosphorization are realized by utilizing the actions of short-cut nitrification, ANAMMOX and denitrifying bacteria, but the whole process is difficult to couple in one reactor due to the difference of strain survival conditions, so that the process is difficult to popularize and apply; in the prior art, a method for realizing the integration of deep denitrification and dephosphorization and coupling denitrification and dephosphorization processes has not been proposed yet. In addition, in the above process, organic carbon sources are consumed in the denitrification process; nitrite produced by short-cut nitrification needs to provide electron donors for denitrifying phosphorus removal bacteria and ANAMMOX bacteria simultaneously, which is easy to cause the deficiency of the electron donors, and the defects can also cause the inhibition of the nitrogen and phosphorus removal effect of the process.
Disclosure of Invention
The invention solves the technical problem that the prior art lacks an integrated deep denitrification and dephosphorization method, and further provides a method and a device for realizing the deep denitrification and dephosphorization by PD/A in-situ coupling algae, which can realize synchronous and efficient removal of nitrogen and phosphorus, save organic carbon source consumption, and further realize carbon dioxide recycling of a bioreactor and remove part of organic nitrogen.
The technical scheme adopted for solving the technical problems is as follows:
a PD/A in-situ coupling algae deep denitrification and dephosphorization method comprises the steps of sending sewage into a short-range denitrification-anaerobic ammoxidation reaction zone for treatment; algae are enriched in the short-range denitrification-anaerobic ammoxidation reaction zone, and a photocatalytic material is added; adding acetic acid into the sewage as a carbon source;
in the treatment process, visible light is used for carrying out illumination on the short-range denitrification-anaerobic ammonia oxidation reaction zone, and the quantity of algae is controlled by adjusting illumination parameters, so that the dissolved oxygen concentration of the short-range denitrification-anaerobic ammonia oxidation reaction zone is kept to be 0.9-1.5mg/L, and ammonia oxidizing bacteria are generated in situ.
The photocatalytic material is C 3 N 4 A material.
The photocatalytic material is C modified by 2, 4-dihydroxypyrimidine molecules 3 N 4 A material.
The algae is generated in situ in the short-range denitrification-anaerobic ammonia oxidation reaction zone, and the relative content ratio Chl-a of the algae and the sludge is as follows: MLVSS is 0.25-0.5mg/g.
The adding amount of acetic acid in the sewage is 10mg TOC/L; the temperature in the reactor is 34.5-35.5 ℃, and the pH value of the inlet water is 7.0-7.5.
The starting process of the short-range denitrification-anaerobic ammoxidation reaction zone is as follows: inoculating activated sludge with good operation of short-cut denitrification-anaerobic ammoxidation, taking sewage containing nitrate, ammonia nitrogen, inorganic salt and microelements or simulated sewage as water inlet, adding acetic acid as a carbon source, maintaining the temperature of a reaction zone at 34.5-35.5 ℃ and the pH value of the water inlet at 7.0-7.5, and operating under stirring condition until the removal rate of nitrite and ammonia nitrogen reaches more than 85%; 150-200mg/L of C is added into the reaction zone 3 N 4 The material is used for controlling illumination parameters, and the reaction is operated until the in-situ enrichment of algae is completed and the denitrification rate reaches more than 90%.
In the starting process, the mol ratio of ammonia nitrogen to nitrate nitrogen in the sewage containing nitrate, ammonia nitrogen, inorganic salt and microelements or the simulated sewage serving as inflow water is about 1:1.
A device for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae, comprising: the reactor is provided with a water inlet and a water outlet, a short-range denitrification-anaerobic ammonia oxidation reaction zone is arranged in the reactor, algae are enriched in the short-range denitrification-anaerobic ammonia oxidation reaction zone, photocatalytic materials are added in the short-range denitrification-anaerobic ammonia oxidation reaction zone, and a stirring device is further arranged in the short-range denitrification-anaerobic ammonia oxidation reaction zone; the carbon source adding device is used for adding a carbon source into the reactor; the visible light irradiation device irradiates the short-range denitrification-anaerobic ammoxidation reaction zone; controlling the quantity of algae by adjusting illumination parameters, so that the dissolved oxygen concentration of the short-range denitrification-anaerobic ammonia oxidation reaction zone is 0.9-1.5mg/L, and generating ammonia oxidizing bacteria in situ.
The visible light irradiation device includes: the dissolved oxygen probe is positioned in the short-range denitrification-anaerobic ammoxidation reaction zone; the pulse light source component is connected with the dissolved oxygen probe through the controller, and the illumination frequency and the illumination duration are controlled by taking the detection value of the dissolved oxygen probe as an index.
A plurality of grooves are formed in the inner wall of the middle lower part of the reactor, each groove extends along the vertical direction, the inner wall surfaces of the plurality of groove rings are uniformly distributed, and the pulse light source assembly is fixed in each groove; the illumination intensity of the pulse light source component is 3000-8000lux.
The method for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae combines microalgae and bacteria to form a bacteria-algae symbiotic PD/A-PN/A (short-cut nitrification-anaerobic ammonia oxidation) system. The technical bottleneck of constructing the algae symbiotic system in the invention is that the settlement performance of sludge flocs is poor due to small cell individuals and gravity of algae, and especially the biomass is reduced due to the fact that algae easily flows out along with effluent, so that the algae symbiotic system is usually accompanied with the problem that algae is not easy to fix, and solidification of algae needs to be completed by virtue of filler carriers and the like. And the algae has low light energy utilization rate, and is difficult to carry out photosynthesis due to insufficient light capturing capabilityFor growth and propagation, cannot be enriched in situ in the bioreactor to form a natural coupling with the flora. In order to solve the problem, the application adds a photocatalytic material in the symbiotic system, and under the illumination condition, the photocatalytic material can efficiently capture light energy and generate photo-generated electrons to reduce CO 2 。
Therefore, the photocatalytic material can be used as a light trapping agent to assist algae to carry out photosynthesis so as to overcome the defect of difficult light energy trapping of the algae. At the same time for CO 2 Fixation also contributes to some extent; and the photocatalytic function of the photocatalytic material activates algae spores to quickly divide in water, so that the growth of cells is promoted, algae is in-situ enriched and attached to the outer surface of a flora and the wall of a reactor, and algae immobilization is naturally formed, so that the conventional algae immobilization program such as a fungus and algae symbiotic filling filler carrier is omitted, and the economic cost is saved. Therefore, the invention utilizes the light capturing characteristic of the photocatalytic material to induce algae to be enriched in situ and immobilized naturally in the bioreactor, and solves the technical bottlenecks of difficult coupling of a bacteria-algae symbiotic system, long starting time of the reactor, poor bacteria-algae immobilization effect and difficult capturing of algae light energy. The photocatalytic function activates rapid separation of algae spores, and is analyzed by a mechanism that photo-generated electrons generated by the photocatalytic material can pass through holes on a cell membrane to enter cells, and the metabolic activity of the cells is stimulated by participating in the electron transfer of the cells.
The invention preferably uses C 3 N 4 As the photocatalytic material and the light trapping agent, nano-sized C is more preferably used 3 N 4 。C 3 N 4 As a semiconductor photocatalysis material with a proper electronic energy band structure, the semiconductor photocatalysis material has good thermal stability and chemical stability, low price, low preparation cost and good biocompatibility, and can not produce toxic action on the growth of microorganisms. And compared with other photocatalytic materials, C 3 N 4 The system performance of the nano particles is better, and a more efficient and stable mycosis PD/A-PN/A system can be quickly constructed. Further, the present invention preferably uses C modified with 2, 4-dihydroxypyrimidine molecules 3 N 4 As a photocatalytic material, the material can further improve the efficiency of constructing a stable mycothroughout PD/A-PN/A system.
The PD/A in-situ coupling algae deep denitrification and dephosphorization method is a synchronous deep denitrification and dephosphorization process technology suitable for various water qualities:
(1) The PD/A in-situ coupling algae deep denitrification and dephosphorization method increases the dephosphorization efficiency for the reaction system, and can achieve the efficient removal of phosphorus in sewage through excessive phosphorus absorption of algae physiological metabolism. The invention takes phosphorus into the reaction system excessively through the introduction of algae, so that the phosphorus removal rate of the original PDA system, which is close to zero, is improved to about 90%, the synchronous deep denitrification and dephosphorization in a single reactor is realized, a dephosphorization unit is not required to be additionally arranged in the actual engineering, and the economic cost is greatly saved.
(2) The method for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae can strengthen the denitrification effect of a PD/A system. In the bacterial and algal symbiotic system constructed by the invention, the AOB flora (ammonia oxidizing bacteria) grows in situ under the micro-oxygen state by utilizing oxygen released by algae, and a PD/A-PN/A coupling process is gradually formed, wherein the AOB flora in the PN process can convert NH 4 + Oxidation of N to NO 2 - N, a new way of nitrous source is provided for anaerobic ammoxidation to optimize the nitrous accumulation effect of the original system, strengthen the denitrification performance of the system, and enable the system to be simultaneously applicable to the treatment of high ammonia nitrogen wastewater, high nitrate nitrogen wastewater and other wastewater with various water qualities. Compared with the denitrification of algae and the denitrification of single microbial flora, the PD/A-PN/A system based on the zoonotic algae constructed by the invention strengthens the ammonia nitrogen conversion effect and provides sufficient nitrite sources for the ANAMMOX process, and finally realizes the ammonia nitrogen removal rate and nitrate removal rate close to 100%, thereby greatly improving the total nitrogen removal effect.
In addition, the invention can realize the removal of a certain amount of organic nitrogen while strengthening the removal effect of inorganic nitrogen by constructing the algae symbiotic system. Algae in the symbiotic system of the invention can absorb inorganic nitrogen in water as nutrientIn addition to the nutrient, organic nitrogen such as urea can be consumed and utilized by assimilation, so that the present invention is realized by C 3 N 4 The PD/A-PN/A system constructed by in-situ enrichment of algae in the PD/A reactor can realize removal of organic nitrogen in water and strengthen the water quality purification effect.
(3) The PD/A in-situ coupling algae deep denitrification and dephosphorization method can save organic carbon source consumption. On one hand, AOB bacteria in PN process in the PD/A-PN/A system constructed by the invention play a role to supplement a new way of nitrite accumulation, thereby reducing the pressure of PD sections in the original PD/A system; on the other hand, the algae is digested in the dark reaction to generate a large amount of micromolecular organisms for providing carbon sources for PD, so that the amount of the additional carbon sources is reduced.
(4) The PD/A in-situ coupling algae deep denitrification and dephosphorization method can realize the carbon dioxide recycling of the bioreactor. The invention utilizes C 3 N 4 The light energy is captured efficiently, the algae is stimulated to be enriched in situ in the reactor, and CO is generated through photosynthesis of the algae 2 Fixing into various organic matters; in addition, the photo-generated electrons generated by the photo-catalytic material can activate the WLP (Wood Ljungdahl pathway) carbon fixation process in anammox bacteria, and the anammox bacteria carbon fixation and algae carbon fixation form a multiple carbon fixation path in the system, so that the fixation of carbon dioxide is effectively enhanced, and the carbon emission reduction is promoted.
In order to make the technical scheme of the method and the device for realizing the deep denitrification and dephosphorization by the PD/A in-situ coupled algae more clearly understood, the invention is further described in detail below with reference to the accompanying drawings and the specific embodiments.
Drawings
FIG. 1 is a schematic structural diagram of a PD/A in-situ coupled algae deep denitrification and dephosphorization device according to the invention;
wherein the reference numerals are as follows:
1-a carbon source water inlet barrel; 2-a sewage water inlet barrel; 3-a liquid inlet water pump; 4-stirring device; 5-time-controlled switch; 6-reactor constant temperature water jacket; 7-discharging a bucket; 8-a controller; 9-dissolved oxygen probe.
Detailed Description
The embodiment provides a device for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae, which is shown in figure 1 and comprises an intermittent reactor (PD/A-SBR reactor), wherein the effective volume of the reactor is 6L, the reactor is provided with a water inlet and a water outlet, and a reactor constant-temperature water jacket 6 is arranged on the wall of the reactor; the upstream of the reactor is provided with a carbon source adding device, the carbon source adding device comprises a carbon source water inlet barrel 1, and acetic acid carbon source is added into water in the carbon source water inlet barrel 1. The upstream of the reactor is also provided with a sewage water inlet barrel 2, the sewage water inlet barrel 2 and the carbon source water inlet barrel 1 are respectively communicated to a main water inlet pipeline through branch pipelines, the branch pipelines are provided with a liquid inlet pump 3, and a time control switch 5 is arranged on the liquid inlet pump 3 and used for automatically controlling the on-off of the water pump. The water outlet of the total water inlet pipeline is communicated with the water inlet of the reactor. The water enters the reactor to form a reaction zone, and a stirring device 4 is arranged in the reaction zone of the reactor. A water outlet barrel 7 is arranged at the downstream of the reactor, and the water discharged from the water outlet enters the water outlet barrel 7.
The device is provided with a visible light irradiation device, the visible light irradiation device comprises a pulse light source component, the pulse light source component is installed on the inner wall of the reactor, a plurality of grooves are formed in the inner wall of the middle lower portion of the reactor, each groove extends in the vertical direction, the inner wall faces of the grooves are uniformly distributed, and the pulse light source component is fixed in each groove. The visible light illumination intensity range of the pulse light source component is 3000-8000lux. The reactor is internally provided with a dissolved oxygen probe 9, the dissolved oxygen probe 9 extends to the lower part of the reactor, the pulse type light source component is connected with the dissolved oxygen probe 9 through a controller 8, and the controller 8 controls illumination frequency and duration by taking a detection value of the dissolved oxygen probe 9 as an index.
Example 1
The embodiment provides a denitrification and dephosphorization method of a device for realizing deep denitrification and dephosphorization based on PD/A in-situ coupling algae shown in figure 1. The PD/A in-situ coupled algae deep denitrification and dephosphorization method in the embodiment takes simulated wastewater as a treatment object and comprises two stages of starting and treatment.
The device for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae is started by the following steps:
inoculating PD/A activated sludge into a reactor, controlling the suspension solid concentration of the sludge mixture to be 4.5-5.5g/L, and operating parameters of the reactor are as follows: the temperature is 34.5-35.5 ℃, the pH of the inlet water is 7.0-7.5, the artificially synthesized sewage is taken as the inlet water, the hydraulic retention time is 12h, and the rotating speed of the stirring device is 700+/-5 rpm. The operation time sequence of the PD/A-SBR reactor is 10min of water inlet, 660min of stirring, 20min of precipitation, 10min of water drainage and 50% of water drainage ratio.
When the artificial synthetic sewage is prepared, water is used as a solvent, inorganic matters and 1ml of trace element solution are added into the water, wherein the inorganic matters are added into each liter of water in the following amount: 1000mg KHCO 3 The method comprises the steps of carrying out a first treatment on the surface of the 60mg MgSO 4 ·5H 2 O;60mg CaCl 2 ·2H 2 O。
The concentrations of the individual microelements in the microelement solution are as follows: EDTA,15000 mg.L -1 ；CoCl 2 ·6H 2 O，240mg·L -1 ；ZnSO 4 ·7H 2 O，430mg·L -1 ；MnCl 4 ·H 2 O，990mg·L -1 ；NaMoO 4 ·2H 2 O，220mg·L -1 ；CuSO 4 ·5H 2 O，250mg·L -1 ；NaSeO 4 ·10H 2 O,210mg·L -1 ；NiCl·6H 2 O，190mg·L -1 ；H 3 BO 4 ，14mg·L -1 。
The reactor is operated according to the operation parameters, the content ratio of ammonia nitrogen to nitrate in the feed water is kept to be 1:1, and the carbon source concentration of acetic acid in the feed water is kept to be 45mg TOC.L -1 About, namely, adding 45mg of TOC-equivalent carbon acetate source into each liter of water. The method is operated for one period every day, the operation condition is kept for a long time until the 15 th day, the nitrite conversion rate and the ammonia nitrogen removal rate of the reactor are both over 85 percent through monitoring, the nitrate of the effluent is lower than about 5mg/L, and the reactor starts to be fed with the effluentC is added according to the addition amount of 180mg/L 3 N 4 The material, namely 180mg of nano-scale C is added into each liter of water 3 N 4 The material is introduced with illumination by a visible light irradiation device in the reactor, and enters an in-situ algae enrichment stage. C added in this example 3 N 4 The material is nano-grade pure g-C prepared by a laboratory thermal polymerization method 3 N 4 The material is in powder form, and the nano-grade pure g-C is before use 3 N 4 The material is first dried in a drying oven. As an alternative embodiment, commercially available nanoscale pure g-C can also be used 3 N 4 A material. The nano-scale pure g-C 3 N 4 After the material is characterized by the technologies of an X-ray diffractometer (XRD), a Scanning Electron Microscope (SEM), a Transmission Electron Microscope (TEM), an X-ray photoelectron spectroscopy (XPS) and the like, the result shows that the prepared material has good photocatalytic performance.
Completion of g-C 3 N 4 After the material is added, the operation parameters of the reactor are kept unchanged, the pulse light source assemblies uniformly distributed at the middle and lower parts of the reactor are utilized for illumination, the light source assemblies irradiate upwards from the middle and lower parts of the reactor, the uniform and omnibearing illumination of the sludge of the reactor is achieved, and the illumination frequency and the illumination time of the pulse illumination are quantitatively adjusted through the controller. The interval of the pulse irradiation time in this embodiment is set to 1h, and the total light-dark time ratio is set to 12h: and (3) adjusting the light intensity of the light source assembly to 6000lux for 12 hours. Maintaining this mode with the two reactors running simultaneously, it was observed that the reactors reached a considerable amount of algae enrichment at day 25, relative algae content Chl-a: MLVSS rises and is maintained at about 0.35, the denitrification rate of the system reaches more than 90%, the total phosphorus removal rate reaches 70%, and the system starts to enter a pulse-type illumination mode adjusting and bacteria and algae comprehensive efficiency optimizing stage based on the condition that the concentration of bacteria and algae biomass is stable and the overall efficiency of the system is obviously improved.
The stage is along with the change of DO content in the reactor, and the frequency of pulse illumination is regulated by a controller, so that the dissolved oxygen concentration of the reactor is kept to be 0.9-1.5mg/L. In this example, the total light-dark time ratio at this stage is 6h:18h, the light intensity is 4000lux, the photosynthesis intensity is controlled in this way, the balance of biomass of algae and flora in the reactor is maintained, the reactor is always kept in a micro-aerobic state, the AOB flora is enriched and grown in situ in the micro-aerobic state, algae is successfully enriched in situ, and a stable running zoon-algae symbiotic PD/A-PN/A system is formed, so that the starting process is completed.
After the reactor completes the starting process, the method for realizing deep denitrification and dephosphorization based on PD/A in-situ coupling algae of the device comprises the following steps:
the sewage is sent into a reactor for treatment, acetic acid is also added as a carbon source, the adding amount of the acetic acid is reduced to 10mg TOC/L, and the nitrate removal rate reaches 100% under the concentration of the carbon source, which indicates that the current carbon source amount can meet the high metabolic activity of denitrifying bacteria of the system; the temperature in the reactor is still kept at 34.5-35.5 ℃, the pH of the inlet water is 7.0-7.5, the hydraulic retention time is 12h, and the rotating speed of the stirring device is 700+/-5 rpm. The PD/A-SBR reactor has the operation time sequence of 10min of water inflow, 660min of stirring, 20min of precipitation, 10min of water drainage and operation under the condition of 50% of water drainage ratio. The pulse illumination frequency is regulated by the controller, and the concentration of the dissolved oxygen in the reactor is kept to be 0.9-1.5mg/L.
The sewage treated in this example was simulated sewage, which was prepared by adding inorganic substances and 1ml of a trace element solution to water with water as a solvent in the preparation. The amount of inorganic substances added to each liter of water was: KNO (KNO) 3 ，43mg；NH 4 Cl，43mg；KH 2 PO 4 ，30mg；KHCO 3 ，1000mg；MgSO 4 ·5H 2 O，60mg；CaCl 2 ·2H 2 O，60mg。
The concentrations of the individual microelements in the microelement solution are as follows: EDTA,15000 mg.L -1 ；CoCl 2 ·6H 2 O，240mg·L -1 ；ZnSO 4 ·7H 2 O，430mg·L -1 ；MnCl 4 ·H 2 O，990mg·L -1 ；NaMoO 4 ·2H 2 O，220mg·L -1 ；CuSO 4 ·5H 2 O，250mg·L -1 ；NaSeO 4 ·10H 2 O，210mg·L -1 ；NiCl·6H 2 O，190mg·L -1 And H 3 BO 4 ，14mg·L -1 。
In 40 days of sewage treatment process operation, the effluent quality is detected every day, and the result shows that the algae symbiotic PD/A-PN/A system formed by the induction of the photocatalytic material realizes the high-efficiency sewage nitrogen and phosphorus removal effect, the ammonia nitrogen removal rate is continuously increased in the operation period and finally the complete removal of ammonia nitrogen is realized, the total nitrogen removal rate is maintained to be more than 95% in 30-40 days, and meanwhile, the total phosphorus removal rate is as high as 90%. In the method for deeply denitrifying and dephosphorizing sewage in the embodiment, the ammonia nitrogen conversion rate and the phosphorus removal efficiency of the system are obviously improved, and compared with a PD/A system, the consumption of additional carbon sources is greatly reduced from 45mg TOC/L to 10mg TOC/L. In addition, the sedimentation performance of the activated sludge in the reactor is obviously improved, and SVI (static var. Sup. I) 5 Significantly decrease from 60.2+/-1.2 mL g -1 SS was reduced to 31.1.+ -. 1.3mL g -1 And SS. The experimental results show that synchronous deep denitrification and dephosphorization can be realized by carrying out in-situ enrichment on algae by adding a photocatalyst material and pulse illumination to establish a PD/A-PN/A system, and the activated sludge has excellent characteristics and enhanced activity.
Example 2
The same PD/A in-situ coupled algae apparatus used in this example was used to achieve deep denitrification and dephosphorization as described in example 1. In the method for realizing deep denitrification and dephosphorization based on PD/A in-situ coupling algae of the device in the embodiment, simulated wastewater is also taken as a treatment object, and the method comprises two stages of starting and treatment.
The starting process of the device for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae in the embodiment is as follows:
inoculating PD/A activated sludge into a batch reactor, controlling the suspension solid concentration of the sludge mixture to be 4.5-5.5g/L, and operating parameters of the reactor are as follows: the temperature is 34.5-35.5 ℃, the pH of the inlet water is 7.0-7.5, the artificially synthesized waste water is taken as the inlet water, the hydraulic retention time is 12h, and the rotating speed of the stirring device is 700+/-5 rpm. The operation time sequence of the batch reactor is 10min of water inlet, 660min of stirring, 20min of precipitation and 10min of water discharge, and the water discharge ratio is 50%.
The said artificial workWhen the synthetic wastewater is prepared, water is used as a solvent, inorganic matters and 1ml of trace element solution are added into the water, wherein the inorganic matters are added into each liter of water in the following amounts: 1000mg KHCO 3 The method comprises the steps of carrying out a first treatment on the surface of the 60mg MgSO 4 ·5H 2 O;60mg CaCl 2 ·2H 2 O。
The concentration of each trace element in the trace element solution is as follows: EDTA,15000 mg.L -1 ；CoCl 2 ·6H 2 O，240mg·L -1 ；ZnSO 4 ·7H 2 O，430mg·L -1 ；MnCl 4 ·H 2 O，990mg·L -1 ；NaMoO 4 ·2H 2 O，220mg·L -1 ；CuSO 4 ·5H 2 O，250mg·L -1 ；NaSeO 4 ·10H 2 O,210mg·L -1 ；NiCl·6H 2 O，190mg·L -1 ；H 3 BO 4 ，14mg·L -1 。
According to the operation mode, the content ratio of ammonia nitrogen to nitrate in the feed water is kept to be 1:1, and the concentration of the carbon source of acetic acid in the feed water is 45mg TOC.L -1 And (3) operating for one period every day, keeping the operating condition for a long time until the 15 th day, wherein the nitrite conversion rate and the ammonia nitrogen removal rate of the reactor reach more than 85%, the nitrate of the effluent is lower than about 5mg/L, at the moment, adding CdS nano particles into the reactor according to the adding amount of 180mg/L, and introducing illumination by using a visible light irradiation device of the PD/A-SBR reactor to enter an in-situ algae enrichment stage. The CdS nanoparticles in this example were prepared in the laboratory using a solvothermal method, and the CdS nanoparticles were dried in a dry box prior to use. After being characterized by the technologies of an X-ray diffractometer (XRD), a Scanning Electron Microscope (SEM), a Transmission Electron Microscope (TEM), an X-ray photoelectron spectroscopy (XPS) and the like, the result shows that the prepared CdS nano particles have good photocatalytic performance.
The operation parameters of the reactor are kept unchanged, the pulse light source assemblies uniformly distributed at the middle and lower parts of the reactor are utilized for illumination, the light source assemblies irradiate upwards from the middle and lower parts of the reactor, the uniform and omnibearing illumination of the sludge in the reactor is achieved, the illumination frequency and illumination time of the pulse illumination are quantitatively adjusted through the controller, the interval of the pulse illumination time in the embodiment is set to be 1h, and the total light-dark time ratio is set to be 12h: and (3) adjusting the light intensity of the light source assembly to 6000lux for 12 hours. The two reactors are kept to run synchronously in this mode, and the reactor is observed to start to enter the stage of optimizing the comprehensive performance of the algae on the 30 th day, and the relative algae content Chl-a is shown at the moment: MLVSS reaches and stabilizes around 0.3, the total nitrogen removal rate is increased to 88%, and the total phosphorus removal rate is broken through from zero to 55%.
The stage is along with the change of DO content in the reactor, and the pulse illumination frequency is regulated by a controller to keep the dissolved oxygen concentration of the reactor at 0.9-1.5mg/L. In this embodiment, the total light-dark time ratio at this stage is 6h:18h, the light intensity is 4000lux, the photosynthesis intensity is controlled in this way, the balance of biomass of algae and flora in the reactor is maintained, the reactor is always kept in a micro-aerobic state, the AOB flora is enriched and grown in situ in the micro-aerobic state, algae is successfully enriched in situ, and a stable running zoon-algae symbiotic PD/A-PN/A system is formed, so that the starting process is completed.
The method for realizing deep denitrification and dephosphorization based on PD/A in-situ coupling algae of the device in the embodiment comprises the following steps:
when the reactor is started, the sewage is sent into the reactor for treatment, and acetic acid is also added as a carbon source, in the embodiment, in order to make the nitrate removal rate approach to 100%, 15mg TOC/L of acetic acid is needed, and the nitrate removal rate is reduced when the adding amount is lower than the adding amount. The temperature in the reactor is still kept at 34.5-35.5 ℃, the pH of the inlet water is 7.0-7.5, the hydraulic retention time is 12h, and the rotating speed of the stirring device is 700+/-5 rpm. The PD/A-SBR reactor has the operation time sequence of 10min of water inflow, 660min of stirring, 20min of precipitation, 10min of water drainage and operation under the condition of 50% of water drainage ratio. The pulse illumination frequency is regulated by the controller, and the concentration of the dissolved oxygen in the reactor is kept to be 0.9-1.5mg/L.
The sewage used in this example was simulated wastewater prepared by adding inorganic substances and 1ml of trace element solution to water in the presence of water as a solvent. The amount of inorganic substances added to each liter of water was: KNO (KNO) 3 ，43mg；NH 4 Cl，43mg；KH 2 PO 4 ，30mg；KHCO 3 ，1000mg；MgSO 4 ·5H 2 O，60mg；CaCl 2 ·2H 2 O，60mg。
The concentration of each trace element in the trace element solution is as follows: EDTA,15000 mg.L -1 ；CoCl 2 ·6H 2 O，240mg·L -1 ；ZnSO 4 ·7H 2 O，430mg·L -1 ；MnCl 4 ·H 2 O，990mg·L -1 ；NaMoO 4 ·2H 2 O，220mg·L -1 ；CuSO 4 ·5H 2 O，250mg·L -1 ；NaSeO 4 ·10H 2 O，210mg·L -1 ；NiCl·6H 2 O，190mg·L -1 And H 3 BO 4 ，14mg·L -1 。
In 40 days of sewage treatment process operation, the effluent quality is detected every day, and the result shows that the algae symbiotic PD/A-PN/A system formed by the induction of the photocatalytic material realizes the high-efficiency sewage nitrogen and phosphorus removal effect, the nitrogen removal efficiency of the system is continuously optimized in the operation period of the reactor, the ammonia nitrogen removal rate is finally kept to be more than 95%, the total nitrogen removal rate is increased and maintained to be more than 90%, and the total phosphorus removal rate reaches 90%. The consumption of the additional carbon source is greatly reduced from 45mg TOC/L to 15mg TOC/L. SVI 5 Significantly decrease from 59.5+ -0.9 mL.g -1 SS was reduced to 33.4.+ -. 1.4mL g -1 SS。
The visible light catalyst g-C in example 1 compared to the CdS nanoparticles in example 2 3 N 4 The system performance of the nano particles is better, and a more efficient and stable mycosis PD/A-PN/A system can be quickly constructed.
Example 3
The same PD/A in-situ coupled algae apparatus used in this example was used to achieve deep denitrification and dephosphorization as described in examples 1 and 2. In the method for realizing deep denitrification and dephosphorization based on PD/A in-situ coupling algae of the device in the embodiment, simulated wastewater is also taken as a treatment object, and the method comprises two stages of starting and treatment.
The starting process of the device for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae in the embodiment is as follows:
inoculating PD/A activated sludge into a batch reactor, controlling the suspension solid concentration of the sludge mixture to be 4.5-5.5g/L, and operating parameters of the reactor are as follows: the temperature is 34.5-35.5 ℃, the pH of the inlet water is 7.0-7.5, the artificially synthesized waste water is taken as the inlet water, the hydraulic retention time is 12h, and the rotating speed of the stirring device is 700+/-5 rpm. The operation time sequence of the batch reactor is 10min of water inlet, 660min of stirring, 20min of precipitation and 10min of water discharge, and the water discharge ratio is 50%.
When the artificial synthetic wastewater is prepared, water is used as a solvent, and inorganic matters and 1ml of trace element solution are added into the water to prepare the artificial synthetic wastewater. The amount of inorganic substances added to each liter of water was: 1000mg KHCO 3 The method comprises the steps of carrying out a first treatment on the surface of the 60mg MgSO 4 ·5H 2 O;60mg CaCl 2 ·2H 2 O。
The concentration of each trace element in the trace element solution is as follows: EDTA,15000 mg.L -1 ；CoCl 2 ·6H 2 O，240mg·L -1 ；ZnSO 4 ·7H 2 O，430mg·L -1 ；MnCl 4 ·H 2 O，990mg·L -1 ；NaMoO 4 ·2H 2 O，220mg·L -1 ；CuSO 4 ·5H 2 O，250mg·L -1 ；NaSeO 4 ·10H 2 O,210mg·L -1 ；NiCl·6H 2 O，190mg·L -1 ；H 3 BO 4 ，14mg·L -1 。
The reactor operates according to the parameters, the content ratio of ammonia nitrogen to nitrate in the feed water is kept to be 1:1, and the carbon source concentration of acetic acid in the feed water is kept to be 45mg TOC.L -1 About, one cycle is operated every day, the operation condition is kept for a long time until the 15 th day, the nitrite conversion rate and the ammonia nitrogen removal rate of the reactor reach more than 85%, the nitrate of the effluent is lower than about 5mg/L, and at the moment, modified g-C is added into the reactor according to the addition amount of 180mg/L 3 N 4 And (3) introducing illumination by using a light source device on the reactor to enter an in-situ algae enrichment stage. Modified g-C added in this example 3 N 4 2, which is prepared by a laboratory pyrolysis method,4-dihydroxypyrimidine molecule modified C 3 N 4 The material is prepared by the following steps: 50mg of 2, 4-dihydroxypyridine is added into 20g of urea, the mixture is transferred into a covered alumina crucible after being uniformly stirred mechanically, the crucible with the sample is placed into a muffle furnace, the temperature rising rate of 5 ℃/min is set from room temperature to 550 ℃, and the temperature is kept for 2h. After the procedure is finished, naturally cooling to room temperature, taking out the obtained light yellow massive solid sample, grinding the light yellow massive solid sample by a mortar to obtain the final light yellow powdery C modified by 2, 4-dihydroxypyrimidine molecules 3 N 4 The material, which is in the order of microns in size, is dried in a dry box prior to use.
Finishing the modification of g-C 3 N 4 After the particles are added, the operation parameters of the reactor are kept unchanged, the visible light source assemblies uniformly distributed at the middle and lower parts of the transparent constant-temperature water jacket of the reactor are utilized for illumination, the light source assemblies irradiate upwards from the middle and lower parts of the reactor, the sludge of the reactor is uniformly and comprehensively irradiated, the illumination frequency and the illumination time of pulse irradiation are quantitatively regulated through the controller, the interval of the pulse irradiation time in the embodiment is set to be 12 hours, namely, the simulated sunlight is continuously irradiated for 12 hours every day, and the sunlight-dark ratio is 12 hours: 12h, and adjusting the light intensity of the light source assembly to 6000lux; maintaining this mode of operation until day 16, a considerable enrichment of algae on the surface of the reactor flora and on the reactor walls was observed, relative content of algae Chl-a: MLVSS is 0.35, and the denitrification rate of the system is greatly improved to more than 90% compared with the previous stage, and in addition, the total phosphorus removal rate of the system is broken through from zero to 70%, and the system starts to enter a stage of adjusting the pulse illumination mode to optimize the comprehensive performance of bacteria and algae.
The stage is along with the change of DO content in the reactor, and the pulse illumination frequency is regulated by a controller to keep the dissolved oxygen concentration of the reactor at 0.9-1.5mg/L. In this embodiment, the total light-dark time ratio at this stage is 6h:18h, the light intensity is 4000lux, the photosynthesis intensity is controlled in this way, the balance of biomass of algae and flora in the reactor is maintained, the reactor is always kept in a micro-aerobic state, the AOB flora is enriched and grown in situ in the micro-aerobic state, algae is successfully enriched in situ, and a stable running zoon-algae symbiotic PD/A-PN/A system is formed, so that the starting process is completed.
The method for realizing deep denitrification and dephosphorization based on PD/A in-situ coupling algae of the device in the embodiment comprises the following steps:
after the reactor finishes the starting process, the sewage is sent into the reactor for treatment, and acetic acid is also added as a carbon source, wherein the adding amount of the acetic acid is 8mg TOC/L, and the nitrate removal rate reaches 100% under the concentration of the carbon source; the temperature in the reactor is still kept at 34.5-35.5 ℃, the pH of the inlet water is 7.0-7.5, the hydraulic retention time is 12h, and the rotating speed of the stirring device is 700+/-5 rpm. The PD/A-SBR reactor has the operation time sequence of 10min of water inflow, 660min of stirring, 20min of precipitation, 10min of water drainage and operation under the condition of 50% of water drainage ratio. The pulse illumination frequency is regulated by the controller, and the concentration of the dissolved oxygen in the reactor is kept to be 0.9-1.5mg/L.
When the simulated wastewater is prepared, water is used as a solvent, and inorganic matters and 1ml of trace element solution are added into the water to prepare the simulated wastewater. The amount of inorganic substances added to each liter of water was: KNO (KNO) 3 ，43mg；NH 4 Cl，43mg；KH 2 PO 4 ，30mg；KHCO 3 ，1000mg；MgSO 4 ·5H 2 O，60mg；CaCl 2 ·2H 2 O，60mg。
The concentration of each trace element in the trace element solution is as follows: EDTA,15000 mg.L -1 ；CoCl 2 ·6H 2 O，240mg·L -1 ；ZnSO 4 ·7H 2 O，430mg·L -1 ；MnCl 4 ·H 2 O，990mg·L -1 ；NaMoO 4 ·2H 2 O，220mg·L -1 ；CuSO 4 ·5H 2 O，250mg·L -1 ；NaSeO 4 ·10H 2 O，210mg·L -1 ；NiCl·6H 2 O，190mg·L -1 And H 3 BO 4 ，14mg·L -1 。
In 40 days of sewage treatment process operation, the effluent quality is detected every day, and the result shows that the bacterial and algal symbiotic PD/A-PN/A system formed by the induction of the photocatalytic material realizes the efficient sewage nitrogen and phosphorus removal effect, and the reactor is in operationThe nitrogen removal efficiency of the system is continuously optimized in the running period, the ammonia nitrogen removal rate is continuously increased in the running period and finally the complete removal of ammonia nitrogen is realized, the total nitrogen removal rate is increased and maintained to be more than 95%, and the total phosphorus removal rate reaches 90%. The consumption of the additional carbon source is greatly reduced from 45mg TOC/L to 8mg TOC/L. SVI 5 Significantly decrease from 61.3+ -1.1 mL.g -1 SS was reduced to 29.1.+ -. 1.2mL g -1 SS。
Example 4
The same PD/A in-situ coupled algae apparatus used in this example was used to achieve deep denitrification and dephosphorization as described in FIG. 1 and was described in examples 1-3. In the embodiment, the method for realizing deep denitrification and dephosphorization based on the PD/A in-situ coupling algae of the device takes actual industrial wastewater as a treatment object and comprises two stages of starting and treatment.
The starting process of the device for realizing deep denitrification and dephosphorization by PD/A in-situ coupling algae comprises the following steps:
PD/A activated sludge is inoculated into a reactor, the suspension fixed concentration of the sludge mixture is 4.5-5.5g/L, coal gasification industrial wastewater is taken as water inlet, meanwhile acetic acid is taken as an external carbon source, the temperature in the reactor is 34.5-35.5 ℃, the hydraulic retention time is 12h, and the rotating speed of a stirring device is 700+/-5 rpm. The PD/A-SBR reactor has the operation time sequence of 10min of water inflow, 660min of stirring, 20min of precipitation, 10min of water drainage and operation under the condition of 50% of water drainage ratio.
The industrial wastewater is derived from sewage discharged by a coal gas chemical plant, and the water quality condition, the pollutant composition and the content mainly comprise: TN,190-225mg/L; NH (NH) 4 + -N，181-224mg/L；NO 3 - -N,130-155mg/L; COD,485-513mg/L; TP,30-45mg/L; pH 8.2-8.5; suspended Solids (SS), 56-101mg/L. Filtering pretreatment is carried out on industrial wastewater before water inflow, and most suspended matters are removed through filtration.
According to the operation parameters, the reactor is operated for one period every day until the 10 th day, the ammonia nitrogen removal rate of the reactor is stable and rises to more than 80%, the total nitrogen removal rate is stable at about 70%, and the consumption of the carbon acetate source is 20mg TOC/L per period. So far, the operation parameters of the reactor are kept unchanged, and the reaction is reversed180mg/L of modified g-C is added into the reactor 3 N 4 Modified g-C added in this example 3 N 4 C modified by 2, 4-dihydroxypyrimidine molecules prepared by a laboratory pyrolysis method 3 N 4 The material is micron-sized and light yellow powder, and is dried in a drying oven before use. The visible light source components uniformly distributed at the middle and lower parts of the transparent constant temperature water jacket of the reactor are utilized for illumination, the light source irradiates upwards from the middle and lower parts of the reactor, so that the sludge of the reactor is uniformly and omnidirectionally irradiated, the illumination frequency and the illumination time of pulse irradiation are quantitatively regulated through the controller, the interval of the pulse irradiation time in the embodiment is set to be 12h, namely, the simulated sunlight is continuously irradiated for 12h every day, and the sunlight-dark ratio is 12h:12h, and adjusting the light intensity of the light source assembly to 6000lux; maintaining this mode of operation until day 20, a considerable enrichment of algae on the surface of the reactor flora and on the reactor walls, relative content of algae Chl-a, was observed: MLVSS is 0.3, and the denitrification rate of the system is greatly improved to more than 85% compared with the previous stage, and in addition, the total phosphorus removal rate of the system is broken through from zero to 55%, and the system starts to enter a stage of adjusting the pulse illumination mode to optimize the comprehensive performance of bacteria and algae.
This phase was varied with the DO level in the reactor, and the frequency of pulsed light was adjusted by the controller to maintain the Dissolved Oxygen (DO) in the reactor at 0.9-1.5mg/L. In this embodiment, the total light-dark time ratio is 6h:18h, the light intensity is 4000lux, the photosynthesis intensity is controlled in this way, the balance of biomass of algae and flora in the reactor is maintained, the reactor is always kept in a micro-aerobic state, the AOB flora is enriched and grown in situ in the micro-aerobic state, algae is successfully enriched in situ, and a stable running zoon-algae symbiotic PD/A-PN/A system is formed, so that the starting process is completed.
The method for realizing deep denitrification and dephosphorization based on PD/A in-situ coupling algae of the device in the embodiment comprises the following steps:
after the reactor finishes the starting process, sewage is sent into the reactor for treatment, and acetic acid is also added as a carbon source, wherein the adding amount of the acetic acid is 10mg TOC/L; the temperature in the reactor is still kept at 34.5-35.5 ℃, the hydraulic retention time is 12h, and the rotating speed of the stirring device is 700+/-5 rpm. The PD/A-SBR reactor has the operation time sequence of 10min of water inflow, 660min of stirring, 20min of precipitation, 10min of water drainage and operation under the condition of 50% of water drainage ratio. The pulse illumination frequency is regulated by a controller, and the Dissolved Oxygen (DO) of the reactor is kept at 0.9-1.5mg/L.
In this embodiment, the industrial wastewater is still used, and the water quality status, the pollutant composition and the content thereof mainly include: TN,190-225mg/L; NH (NH) 4 + -N，181-224mg/L；NO 3 - -N,130-155mg/L; COD,485-513mg/L; TP,30-45mg/L; pH 8.2-8.5; suspended Solids (SS), 56-101mg/L.
In 40 days of sewage treatment process operation, the effluent quality is detected, and the result shows that the ammonia nitrogen removal rate is maintained to be more than 95%, the total nitrogen removal rate is maintained to be more than 90% up to 100%, the total phosphorus removal rate is as high as 90%, the COD removal effect of the system is finally stabilized to be 98%, and the method for deep denitrification and dephosphorization of sewage in the embodiment remarkably improves the ammonia nitrogen conversion rate and the phosphorus removal efficiency of the system. The sedimentation performance of activated sludge in the reactor is obviously improved, and SVI (static var compensator) 5 From 60.2+ -1.2 mL g -1 SS was reduced to 38.9.+ -. 2.3mL g -1 And SS. From the experimental results, it is proved that the semiconductor photocatalytic material is used for modifying g-C 3 N 4 The system can enrich algae in situ and change the microenvironment of the system through photosynthetic oxygen evolution of the algae, realizes the construction of a PD/A-PN/A system and synchronous deep denitrification and dephosphorization of industrial wastewater with high pollutant concentration, and has good microbial activity and high stability.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the protection scope of the present invention is subject to the claims.
Claims (4)
1. A method for realizing deep denitrification and dephosphorization by coupling algae in situ through short-cut denitrification/anaerobic ammonia oxidation is characterized by comprising the following steps: (1) Inoculating short-cut denitrification/anaerobic ammoxidation activated sludge into a reactor, taking simulated sewage containing nitrate, ammonia nitrogen, inorganic salt and trace elements as water inlet, adding acetic acid as a carbon source, running under stirring until nitrite and ammonia nitrogen removal rates reach more than 85%, and adding 150-200mg/L of photocatalytic material into the reactor, wherein the molar ratio of ammonia nitrogen to nitrate nitrogen in the simulated sewage is 1:1, and the photocatalytic material is g-C 3 N 4 The material keeps the operation parameters of the reactor unchanged, the visible light is introduced by a pulse light source component fixed in a plurality of grooves on the inner wall of the lower part of the reactor, the illumination intensity of the pulse light source component is 3000-8000lux, the illumination parameters are controlled, the reaction is operated until the in-situ enrichment of algae is completed, and the relative content ratio of algae and sludge is Chl-a: MLVSS is 0.25-0.5mg/g, the denitrification rate reaches more than 90%, the total phosphorus removal rate reaches 70%, then the illumination parameter is regulated to control the quantity of algae, so that the dissolved oxygen concentration of a reaction zone is kept to be 0.9-1.5mg/L, ammonia oxidizing bacteria are generated in situ, namely a stable running bacteria-algae symbiotic short-cut denitrification/anaerobic ammonia oxidation-short-cut nitrification/anaerobic ammonia oxidation system is formed, and the starting of the reactor is completed; (2) After the reactor is started, the sewage is sent into the reactor for treatment, acetic acid is added as a carbon source, visible light is regulated, and the concentration of dissolved oxygen in the reactor is kept to be 0.9-1.5mg/L.
2. The method for realizing deep denitrification and dephosphorization by coupling algae in situ through short-cut denitrification/anaerobic ammonia oxidation according to claim 1, wherein the photocatalytic material is g-C modified by 2, 4-dihydroxypyrimidine molecules 3 N 4 A material.
3. The method for realizing deep denitrification and dephosphorization by coupling algae in situ through short-cut denitrification/anaerobic ammonia oxidation according to claim 2, wherein the adding amount of acetic acid in the step (2) is 10mg TOC/L, the temperature in the reactor is 34.5-35.5 ℃, and the pH value of the inlet water is 7.0-7.5.
4. The method for achieving deep denitrification and dephosphorization by coupling algae in situ through short-cut denitrification/anaerobic ammonia oxidation according to claim 3, wherein the temperature of the reactor in the step (1) is maintained at 34.5-35.5 ℃, and the pH of the inlet water is 7.0-7.5.
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