CN114869818B

CN114869818B - Composition with repairing effect and preparation method thereof

Info

Publication number: CN114869818B
Application number: CN202210522935.0A
Authority: CN
Inventors: 曹华; 夏念民
Original assignee: Guangdong Pharmaceutical University Testing Technology Service Co ltd; Miaofen Pharmaceutical Technology Guangzhou Co ltd; Guangdong Pharmaceutical University
Current assignee: Guangdong Pharmaceutical University Testing Technology Service Co ltd; Miaofen Pharmaceutical Technology Guangzhou Co ltd; Guangdong Pharmaceutical University
Priority date: 2022-05-13
Filing date: 2022-05-13
Publication date: 2023-06-20
Anticipated expiration: 2042-05-13
Also published as: CN114869818A

Abstract

The invention discloses a composition with repairing effect and a preparation method thereof, and belongs to the technical field of cosmetics. The composition comprises the following components: purslane extract, stephania root extract and acteoside extract. The purslane extract, the stephania root extract and the calycoma extract are adopted for compounding, the formula composition is simple, the safety is high, the product stability and the corrosion resistance are good, the repairing effect is good, and the market application prospect is good.

Description

Composition with repairing effect and preparation method thereof

Technical Field

The invention relates to the technical field of cosmetics, in particular to a composition with repairing effect and a preparation method thereof.

Background

With the development of society, the skin pressure increases due to the reduction of working pressure and environmental quality, so that the skin barrier is damaged, the skin becomes sensitive, further skin percutaneous water loss is increased, the skin is easy to dry, dry itching is caused, and aging is accelerated. Human skin contains abundant sensory nerve fibers that allow the central nervous system to perceive the condition of the skin at any time and respond to various chemical reactions, heat, and physiological stimuli. When the skin barrier function is damaged, the transdermal water loss can be increased, so that external stimulus is easy to permeate, the signal transmission of the skin sensory nerve is enhanced, the tolerance threshold is reduced, the skin immune response is activated due to weak external stimulus overreaction, the vascular reactivity is enhanced, the vascular expansion and inflammatory cell infiltration are caused, the skin is red and swollen, telangiectasia is caused, and various skin discomfort symptoms such as tightness, burning, itching and the like are perceived. Therefore, cosmetic demands with modified efficacy are increasing.

In the cosmetic market, the Chinese herbal medicine cosmetics account for about 30-40%, so that people can more comprehensively demand the skin care, and cosmetics with naturalness, safety and small side effect are favored by consumers. Because the traditional pure compound has the advantages of high development difficulty, long period, high cost and easy generation of larger irritation, the natural Chinese herbal medicine has the advantages of safer and easier preparation, etc. In addition, since one Chinese medicinal plant contains multiple components, extraction is generally required, and different extraction processes can also lead to large differences in the composition and content of the extract.

For example, chinese patent application CN201910618816.3 discloses a composition with anti-aging and repairing effects, and a preparation method and application thereof. The composition comprises the following components in percentage by weight: apple fruit cell coating 0.1-10.0%, rose extract 0.01-1.0%, littoral celery callus culture filtrate 0.01-5.0%, hexapeptide-11.003-0.006%, palmitoyl tripeptide-1.01-0.5%, palmitoyl tetrapeptide-7.01-0.5%, dipeptide diamino Ding Xianbian-ylamide diacetate 0.01-0.5% and emulsifying agent 0.1-1%. The apple fruit cell wrapper consists of an apple fruit cell active substance, a quince leaf polyphenol extract and a rose extract which permeate into the apple fruit cell active substance, wherein the outer surface of the apple fruit cell active substance is coated with a phospholipid bilayer, and the composition has the functions of resisting aging and repairing and has the characteristics of stable and lasting functions.

At present, the traditional Chinese medicine plant extract is used for preparing cosmetics, which is usually added by taking a traditional Chinese medicine as a synergistic component, not taken as a main functional component, and has limited effect; or multiple traditional Chinese medicine extracts with the same efficacy are overlapped and used, and are generally selected from traditional Chinese medicine components with better effects, and the selection range is limited.

In view of the above, the present application provides a new composition with repairing effect and its preparation method, which simplifies the formulation composition, and uses the combination of traditional Chinese medicine extract to obtain better repairing effect and product characteristics.

Disclosure of Invention

Purslane, alias Ma Xian, wuxing grass, changshui, etc., stems lying flat, spread on the ground, branches light green or dark red. She Husheng the leaves are flat, thick and similar to a dent shape, the upper part is dark green, and the lower part is light green or dark red, and the petioles are thick and short. The plant polysaccharide and vitamins can nourish and lubricate skin, and reduce dead skin and horny layer generation caused by dryness; the amino acid can shrink vascular smooth muscle, has central and peripheral effects, and can relieve skin and inhibit skin itching caused by dryness; the flavone and saponin can remove free radicals, resist oxidation, and delay skin aging.

Radix Stephaniae Japonicae, also called herba Hedyotidis Diffusae, herba Euphorbiae Humifusae, etc. is plant of Stephania of Stephaniae of Fangkeiaceae, grass, fallen leaves, herba Pileae Scriptae, and is usually 1-2 m or more; root clusters or near cones; the small branches are purple red and fine. Leaf paper, triangle oblate to near circle. The polysaccharide component and alkaloid stephanine in radix Stephaniae Cepharanthae have antiinflammatory, relieving and after-sun repairing effects in skin care field.

The verbasc is also named as the tremella, the hairy leaves, the inula root, the tiger tail penis, the king penis and the like, the biennial herb is up to 1.5 meters, and the whole plant is dense and thick light gray yellow star hair. The basal leaves and the lower stem leaves are inverted and cape needle-shaped oblong, the basal parts are gradually narrowed to be short-handled, the basal parts are as long as 15 cm and the basal parts are as wide as 6 cm, the edges are provided with shallow circular teeth, the upper stem leaves are gradually reduced and gradually changed into oblong to oval oblong, and the basal parts are downwards extended to be narrow wings. The extract contains various functional components, has the effects of diminishing inflammation, stopping bleeding, clearing heat, removing toxicity and the like, is used in daily chemical products, has the effects of astringing pores and moistening skin, and can keep the skin in a young state.

The invention aims to provide a novel composition with repairing effect and a preparation method thereof, wherein a purslane (PORTULACA OLERACEA) extract, a stephania root (STEPHANIA CEPHALANTHA) extract and a verbascum (VERBASCUM THAPSUS) extract are compounded to obtain the composition with better repairing effect, and the composition has the advantages of simple formula composition, high safety, good product stability and good corrosion resistance.

In order to achieve the above object, the present invention has the following technical scheme:

first, the present invention provides a composition having a repairing effect, comprising the following components: purslane extract, stephania root extract and acteoside extract.

Preferably, the weight ratio of the purslane extract, the stephania root extract and the verbascum extract is 1-6:0.1-0.3:1-2, further preferably 4:0.2:2.

preferably, the composition with repair efficacy comprises the following ingredients: purslane extract, stephania root extract, acteoside extract, calycosin, polyalcohol, glycerin and water.

Further preferably, the composition with repairing effect comprises the following components in parts by weight:

1-10 parts of purslane extract, 0.05-0.5 part of stephania root extract, 1-10 parts of calycosin extract, 0.1-3 parts of hyaluronic acid, 15-40 parts of polyalcohol, 5-25 parts of glycerin and water.

Still more preferably, the composition with repairing effect comprises the following components in parts by weight:

1-6 parts of purslane extract, 0.1-0.3 part of stephania root extract, 1-2 parts of calycosin extract, 0.5-1 part of hyaluronic acid, 20-35 parts of polyalcohol, 10-20 parts of glycerin and water.

Most preferably, the composition with repairing effect comprises the following components in parts by weight: 4 parts of purslane extract, 0.2 part of white drug extract, 2 parts of verbascum extract, 1 part of hyaluronic acid, 23 parts of polyalcohol, 15 parts of glycerin and water.

Preferably, the preparation method of the purslane extract comprises the following steps: adding ethanol into herba Portulacae, heating for reflux, vacuum filtering, and heating and evaporating supernatant to obtain herba Portulacae extract.

Further preferably, the volume fraction of the ethanol is 50-60%.

Further preferably, the feed liquid ratio of the purslane to the ethanol is 1:5-8, more preferably 1:6.

further preferably, the temperature of the heating reflux is 80-90 ℃ and the time is 1-3h.

Further preferably, the temperature of the heating evaporation is 55 ℃ to 60 ℃.

Further preferably, the heating evaporates to a volume of 1/2 to 1/3 of the original volume.

Preferably, the preparation method of the stephania root extract comprises the following steps: adding ethanol into the radix Stephaniae Cepharanthae powder for extraction, and heating and concentrating to obtain radix Stephaniae Cepharanthae extract.

Further preferably, the volume fraction of the ethanol is 55-65%.

Further preferably, the feed liquid ratio of the white powder to the ethanol is 1:18-22, more preferably 1:20.

further preferably, the extraction time is 3-5 hours.

Further preferably, the temperature of the heat concentration is 50-70 ℃.

Further preferably, the concentration is performed to 1/5 to 1/6 of the original volume.

Preferably, the preparation method of the verbascum extract comprises the following steps: adding methanol into herba Verbasci Thunb for extraction, and concentrating under heating to obtain herba Verbasci Thunb extract.

Further preferably, the volume fraction of methanol is 45-55%.

Further preferably, the feed liquid ratio of the verbascum to the methanol is 1:8-12, more preferably 1:10.

further preferably, the concentration is performed to 1/3 to 1/4 of the original volume.

Preferably, the polyol is at least one selected from 1, 2-pentanediol, butanediol and aminomethylpropanol, more preferably a mixture of 1, 2-pentanediol, butanediol and aminomethylpropanol, and even more preferably, the weight ratio of 1, 2-pentanediol, butanediol and aminomethylpropanol is 10:10:3.

secondly, the invention provides a preparation method of the composition with repairing effect, which comprises the following steps:

(1) Placing the polyol with the formula dosage into a mixing stirrer, heating to 50-70 ℃ while stirring, and preserving heat for 10-20 minutes to obtain an oil phase;

(2) Placing herba Portulacae extract, radix Ardisiae Japonicae extract, herba Verbasci Thunbergii extract, hyaluronic acid and glycerol in a mixing stirrer with deionized water, stirring, and slowly heating to 50-70deg.C to obtain water phase;

(3) And (3) adding the oil phase obtained in the step (1) into the water phase obtained in the step (2) under the high-speed shearing condition, and shearing until the particle size is smaller than 5 mu m to obtain the composition.

Finally, the invention provides application of the composition with the repairing effect in preparing skin care products.

The skin care product is at least one of toner, skin softening water, skin tightening water, nutrition toning water, nutrition cream, massage cream, essence, eye cream, skin cleaning foam, skin cleaning water, facial mask, powder cake, body lotion, body cream, body oil and body milk.

The beneficial effects of the invention are as follows:

the purslane extract, the stephania root extract and the calycoma extract are adopted for compounding, the formula composition is simple, the safety is high, the product stability and the corrosion resistance are good, and the repairing effect is good.

Detailed Description

In order to make the technical means, the creation features, the achievement of the purpose and the effect of the present invention easy to understand, the present invention will be further elucidated with reference to the specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all of them. Based on the examples in the embodiments, those skilled in the art can obtain other examples without making any inventive effort, which fall within the scope of the invention. In the following examples, unless otherwise specified, the methods of operation used were conventional, the equipment used was conventional, and the materials used in the examples were the same.

In the following examples, the radix Stephaniae Cepharanthae powder is obtained by pulverizing radix Stephaniae Cepharanthae with an air jet mill and sieving with 100 mesh sieve, wherein the radix Stephaniae Cepharanthae is YB0028; hyaluronic acid was purchased from the Guangzhou city, yabao chemical Co., ltd, with the product number YBJC018; the water is deionized water.

Example 1 preparation of extract

(1) Taking purslane, adding 55% ethanol, refluxing the purslane and the ethanol at a feed liquid ratio of 1:6 at 85 ℃, filtering, discarding impurities, evaporating supernatant at 55-60 ℃ until the volume of the supernatant is half of the original volume, and obtaining a purslane extract for later use;

(2) Taking white medicinal herb root powder, taking 60% ethanol as an extraction solvent, wherein the feed liquid ratio is 1:20, heating in water bath at 60deg.C for 4 hr, concentrating to obtain extractive solution with volume of 1/5 of original volume, and collecting radix Stephaniae Cepharanthae extractive solution;

(3) Extracting the calycosin with 50% methanol, wherein the feed liquid ratio is 1:10, heating the extract in water bath at 60 ℃, concentrating until the volume of the extract is 1/3 of the original volume, and obtaining the verbascum extract for standby.

Example 2 preparation of the composition

(1) Placing the polyol with the formula dosage into a mixing stirrer, heating to 60 ℃ while stirring, and preserving heat for 15 minutes to obtain an oil phase;

(3) Adding the oil phase into the water phase under high-speed shearing condition, and shearing until the particle size is smaller than 5 mu m to obtain the composition.

Formulations a-Q were as follows (the following formulations were all made up to 100 parts with balance water):

table 1.

Result detection

1. Safety test

The human skin closed patch test was performed according to the human skin patch test specified in cosmetic safety technical Specification (2015 edition). 35 volunteers are selected, and no suspicious reaction and no positive reaction are caused to the formula product of the invention, so that the product of the invention is proved to be safe and has no adverse reaction.

2. Stability detection:

the detection method comprises the following steps: and (3) carrying out cold and hot alternating test, sequentially sealing and preserving the obtained product at 45 ℃,25 ℃ and-15 ℃ for 24 hours, circulating for 5 times, standing at 25 ℃ for 12 hours, thin coating the product and the corresponding product prepared newly by the same method on a black observation plate, and comparing the appearance, wherein the grades are as follows:

table 2.

	Color (transparency)	Precipitation	Layering	Uniformity of application (compared with the original product)
					0	No change	Without any means for	Without any means for	Identical to
1	Slight change	Small amount, difficult discrimination	Slight delamination	Slightly uneven application
					2	Obvious change	Obvious precipitation	Obvious delamination	Obviously difficult to uniformly spread

The test results are shown in the following table:

table 3.

The results in Table 3 show that the formula A-M, P has good stability, and the problems of precipitation, color change, layering and the like are avoided after cold-hot alternating experiments. The white drug extract and the calycosin extract in the formula N, O, Q have high content and poor stability.

3. Antibacterial property detection

The detection method comprises the following steps: sterilizing filter paper at high temperature, preparing round sterile filter paper sheets with diameter of 6mm by using a puncher, respectively placing products prepared by the formulas A-Q into a beaker, and respectively placing the round sterile filter paper sheets (5 sheets) for full soaking. Sterile water dips were also set as controls. A total concentration of about 1.0X10X 10 was aspirated at 0.1ml ⁸ cfu/ml of staphylococcus aureus suspension was spread evenly on the petri dish. And flatly and uniformly attaching the fully soaked round sterile filter paper sheets to a culture dish, placing the filter paper sheets in a constant temperature incubator at 37 ℃ for culturing for 24 hours in an inverted plate mode, and taking out the filter paper sheets to measure the diameter of a bacteria inhibition zone.

D zone of inhibition = D outer ring-D inner ring (sample)

The test results are shown in the following table:

table 4.

As can be seen from the results in table 4, the product prepared by the formulation M, N, O only shows weak antibacterial property, but the antibacterial property is obviously improved after the three components are compounded. Therefore, the composite composition has better antibacterial performance, can reduce the use of cosmetic preservatives and improve the use safety when being used in cosmetics.

4. Repair effect test

The detection method comprises the following steps: percutaneous moisture loss assay

170 volunteers were selected, and women aged 18-40 and having skin dryness problems were randomized into 17 groups of 10. The products prepared by the formulas A-Q are respectively used in each group, when in use, the products are uniformly smeared on the face and are beaten to be completely absorbed, and the products are used once in the morning and evening, 1.5mL each time, and are continuously used for one week without using other skin care products in the period. The percutaneous moisture loss rate was measured horizontally by placing a percutaneous moisture loss meter (Tewameter) probe at the same site on the buccal skin before and after one week of use of the product. Testing of the same subject is performed by the same instrument, the same tester. Before each measurement, the probe was cleaned, while the human subject was allowed to sit still for 10min while washing the face with clear water, and was subjected to the test.

Improved value of percutaneous moisture loss = percutaneous moisture loss before test-percutaneous moisture loss after test the improved value of percutaneous moisture loss was measured for each person and averaged. The detection results are as follows:

table 5.

From the above table, the purslane extract prepared by the invention has a certain repairing effect, but the improving effect is limited, and the white drug extract and the calycosin extract basically have no obvious repairing function under the addition of the invention. But the overall performance of the three materials is obviously improved after the three materials are compounded. The skin barrier water retention capacity of the subjects using the formula A-L is improved, and the skin barrier water retention capacity of the subjects using the formula A-L is obviously reduced, so that the skin barrier water retention agent has good repairing effect.

The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.

Claims

1. A composition having skin rejuvenation effect, characterized by the following composition: 1-6 parts of purslane extract, 0.1-0.3 part of stephania root extract, 1-2 parts of verbascum extract, 0.5-1 part of hyaluronic acid, 20-35 parts of polyalcohol, 10-20 parts of glycerin and water;

the preparation method of the purslane extract comprises the following steps: adding ethanol into herba Portulacae, heating for reflux, filtering, and heating and evaporating supernatant to obtain herba Portulacae extract;

the preparation method of the stephania root extract comprises the following steps: adding ethanol into the radix Stephaniae Cepharanthae powder for extraction, and heating and concentrating to obtain radix Stephaniae Cepharanthae extract;

the preparation method of the verbascum extract comprises the following steps: adding methanol into herba Verbasci Thunb for extraction, and heating and concentrating to obtain herba Verbasci Thunb extract;

the polyalcohol is at least one selected from 1, 2-pentanediol, butanediol and aminomethylpropanol.

2. The composition with skin care effect according to claim 1, wherein in the preparation method of the purslane extract, the volume fraction of ethanol is 50-60%, and the ratio of purslane to ethanol is 1:5-8, heating and refluxing at 80-90 ℃ for 1-3h until the volume is 1/2-1/3 of the original volume;

in the preparation method of the white drug seed extract, the volume fraction of ethanol is 55-65%, and the feed liquid ratio of white drug seed powder to ethanol is 1:18-22, extracting for 3-5h, concentrating to 1/5-1/6 of the original volume;

in the preparation method of the verbascum extract, the volume fraction of methanol is 45-55%, and the feed liquid ratio of the verbascum to the methanol is 1:8-12, concentrating to 1/3-1/4 of the original volume.

3. The skin care composition according to claim 1, wherein the polyhydric alcohol is a mixture of 1, 2-pentanediol, butanediol, and aminomethylpropanol.

4. A composition with skin care efficacy according to claim 3, characterized in that the weight ratio of 1, 2-pentanediol, butanediol, aminomethylpropanol is 10:10:3.

5. use of the composition having skin care effect according to any one of claims 1 to 4 for preparing a skin care product, wherein the skin care product is at least one of a toner, a tightening lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing foam, a cleansing lotion, a mask, a foundation, a body lotion, a body cream, a body oil and a body cream.