CN114847292B - Sterilization composition containing zoxamide and fludioxonil and application thereof - Google Patents
Sterilization composition containing zoxamide and fludioxonil and application thereof Download PDFInfo
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- CN114847292B CN114847292B CN202110156555.5A CN202110156555A CN114847292B CN 114847292 B CN114847292 B CN 114847292B CN 202110156555 A CN202110156555 A CN 202110156555A CN 114847292 B CN114847292 B CN 114847292B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to a bactericidal composition containing zoxamide and fludioxonil, which comprises the active ingredients of zoxamide and fludioxonil in a binary compound way and the balance of auxiliary materials, wherein the bactericidal composition can be prepared into various dosage forms, can be used for preventing and treating various diseases on crops such as vegetables and fruit trees, is especially suitable for preventing and treating gray mold of crops such as flowers, vegetables, rice, wheat, fruit trees, cotton, rape and the like, and preventing and treating epidemic diseases of crops such as Solanaceae, lily and cucurbitaceae, such as pepper epidemic disease, potato late blight and the like. Compared with a single dose, the synergistic bactericidal composition improves the control effect on diseases such as gray mold, epidemic disease and the like of crops, can greatly reduce the dosage, reduce environmental pollution and overcome and delay the generation of drug resistance of germs.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition containing zoxamide and fludioxonil and application thereof.
Background
Zoxamide (zoxamide) is a protective bactericide of benzamide, has a longer lasting period and excellent rain wash resistance, is the only effective medicament for oomycetes in antimitotic bactericides, is mainly used for preventing and treating oomycetes diseases such as potato late blight, tomato late blight, cucumber downy mildew, grape downy mildew and the like, and has higher prevention effect on powdery mildew and gray mold of solanaceae. Chemical name: 3, 5-dichloro-N- (3-chloro-1-ethyl-1-methyl-2-oxopropyl) -4-methylbenzamide. The chemical structural formula is as follows:
fludioxonil (fluxionoil) is a novel non-systemic phenylpyrrole fungicide which has bacteriostatic and bactericidal actions, mainly by inhibiting the metastasis associated with glucose phosphorylation, and thus inhibiting the growth of fungal mycelium, ultimately leading to the death of pathogens. Is mainly used for preventing and controlling fusarium, wheat anabaena, rhizoctonia solani and the like, and has special effect on gray mold. The sterilization mechanism for the botrytis cinerea mainly comprises the steps of dissolving the cell wall of the botrytis cinerea thalli, rapidly destroying the hydrophobic chain on the cell membrane of the botrytis cinerea, oxidizing and dissolving protein which is a main substance of the vital activity of bacteria, and destroying the synthesis of the protein and nucleic acid, thereby interfering the biosynthesis and biological oxidation process of the botrytis cinerea, and the action mechanism has uniqueness and longer lasting period. Fludioxonil has no mutual resistance with the existing bactericide, and is a high-efficiency low-toxicity contact bactericide. Chemical name: 4- (2, 2-difluoro-1, 3-benzodioxy-4-yl) pyrrole-3-carbonitrile. The chemical structural formula is as follows:
gray mold is a fungal disease caused by Botrytis cinerea infection, the fungus hosts are wide, more than 240 plants such as vegetables, fruits, flowers and the like can be infected, and the gray mold can occur in the storage and transportation processes after harvest, and is one of plant diseases with serious harm and wide disease scope in the crop planting and fruit transportation and storage processes. The plant epidemic disease mainly is caused by Phytophthora (Phytophthora) fungi, and can occur on crops such as Solanaceae, liliaceae, cucurbitaceae and the like, such as pepper epidemic disease caused by Phytophthora capsici (P.capsici), potato late blight caused by Phytophthora infestans (P.infestans), tomato late blight and the like. Epidemic diseases are widely distributed throughout the world, and are particularly rampant in high-temperature and high-humidity areas, and can cause destructive damage to plants. The application of chemical agents is the most effective means for controlling botrytis and epidemic diseases of crops. However, long-term continuous high-dose application of a single chemical bactericide is easy to cause problems such as medicament residue, environmental pollution and development of drug-resistant fungi. The reasonable chemical bactericide compound or compounding has the positive characteristics of expanding the bactericidal spectrum, improving the control effect, prolonging the application period, reducing the dosage, reducing the phytotoxicity, reducing the residue, delaying the occurrence and development of the drug resistance of fungi and the like, and the bactericide compound is one of the most effective methods for solving the problems. The price of developing new products of bactericides is continuously increased, and in contrast, the development and research of efficient, low-toxicity and low-residue compound and compound have the advantages of low investment, short development period and the like, and are valued at home and abroad, so that the development and development forces are increased in a dispute. Based on indoor screening and field experiments, the invention screens out that the zoxamide and fludioxonil are compounded, and has obvious synergy. And the bactericidal composition compounded by the zoxamide and the fludioxonil has been recently reported.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a bactericidal composition containing zoxamide and fludioxonil, wherein the active ingredients of the bactericidal composition are two-element compounding of the zoxamide and fludioxonil, and the balance is auxiliary materials.
The invention provides a sterilization composition, which comprises the following components: zoxamide, fludioxonil and auxiliary materials; the active ingredients of the composition are zoxamide and fludioxonil, the weight ratio of the zoxamide to the fludioxonil is (1-80) (80-1), for example, (1-50) (50-1), (1-40) (40-1), (1-30) (30-1), (1-20) (20-1); exemplary are 50:1, 40:1, 30:1, 20:1, 10:1, 5:1, 1:1, 1:5, 1:10, 1:20, 1:30, 1:40, 1:50.
According to an embodiment of the invention, the sum of the weight percentages of zoxamide and fludioxonil is 1-90%, e.g. 10-80%, 15-70%, 20-60%, etc., e.g. 1%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 24%, 30%, 35%, 38%, 42%, 45%, 50%, 55%, 60%, 65%, 68%, 75%, 80%, 85%, 90%, based on the total weight of the bactericidal composition.
According to an embodiment of the present invention, the auxiliary material may be selected from one, two or more of deionized water, an organic solvent, an emulsifier, a dispersant, a wetting agent, a thickener, an antifoaming agent, a stabilizer, a binder, a disintegrant, an antifreeze agent, an anticaking agent, a suspending agent, a film former, a preservative, a colorant, a polymeric wall material, a pH adjuster, a filler, and the like.
According to an embodiment of the present invention, the bactericidal composition may be a liquid formulation or a solid formulation.
According to embodiments of the present invention, the range of the sum of the weight percentages of zoxamide and fludioxonil varies among different formulation types; preferably, the total weight percentage of the zoxamide and fludioxonil in the liquid preparation can be 1-60%, preferably 5-50%; preferably, the sum of the weight percentages of the zoxamide and fludioxonil in the solid preparation may be between 5% and 80%, preferably between 10% and 70%.
The invention also provides a preparation method of the bactericidal composition, which comprises the step of mixing zoxamide, fludioxonil and auxiliary materials to obtain the bactericidal composition.
According to embodiments of the present invention, the bactericidal composition may be diluted before use by a user or used directly. The preparation can be prepared by a processing method known to a person skilled in the art, namely, the effective active ingredient is mixed with one or more of deionized water, an organic solvent, an emulsifier, a dispersing agent, a wetting agent, a thickening agent, a defoaming agent, a stabilizing agent, a binding agent, a disintegrating agent, an antifreezing agent, an anti-caking agent, a suspending agent, a film forming agent, a preservative, a coloring agent, a polymer capsule wall material, a pH regulator or a filler and the like to prepare the bactericidal composition.
According to the embodiment of the invention, the sterilization composition can be in the form of water dispersible granules, dispersible agents, wettable powder, suspending agents, aqueous emulsion, microemulsion, suspending emulsion, microcapsule suspending agents, microcapsule suspending-suspending agents, suspending seed coating agents, emulsifiable concentrates and granules.
According to an embodiment of the invention, the wettable powder comprises the following components in percentage by weight: 1-80% of zoxamide, 1-80% of fludioxonil, 1-12% of dispersing agent, 1-8% of wetting agent and the balance of filler.
According to an embodiment of the invention, the water dispersible granule comprises the following components in percentage by weight: 1 to 80 percent of zoxamide, 1 to 80 percent of fludioxonil, 1 to 12 percent of dispersing agent, 1 to 8 percent of wetting agent, 1 to 10 percent of disintegrating agent and the balance of filler.
According to an embodiment of the invention, the suspending agent comprises the following components in percentage by weight: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 12 percent of dispersing agent, 1 to 10 percent of wetting agent, 0.1 to 8 percent of thickening agent, 0.1 to 8 percent of antifreeze agent and the balance of deionized water.
According to an embodiment of the invention, the aqueous emulsion comprises the following components in percentage by weight: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 60 percent of organic solvent, 1 to 12 percent of emulsifier, 0.1 to 8 percent of antifreeze agent, 0.01 to 2 percent of defoamer, 0.1 to 2 percent of thickener and the balance of deionized water.
According to an embodiment of the invention, the suspended seed coating agent comprises the following components in percentage by weight: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 12 percent of dispersing agent, 1 to 10 percent of wetting agent, 1 to 10 percent of antifreeze agent, 0.1 to 10 percent of anticaking agent, 0.1 to 5 percent of suspending agent, 1 to 10 percent of film forming agent, 0.1 to 5 percent of preservative, 1 to 30 percent of colorant, 0.1 to 5 percent of pH regulator, 0.1 to 8 percent of thickening agent and the balance of deionized water.
According to an embodiment of the invention, the microcapsule suspension comprises the following components in percentage by weight: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 30 percent of high molecular capsule wall material, 2 to 10 percent of dispersing agent, 1 to 50 percent of organic solvent, 1 to 7 percent of emulsifying agent, 0.1 to 5 percent of pH regulator, 0.01 to 2 percent of defoaming agent, 0.1 to 8 percent of thickening agent, 0.1 to 8 percent of antifreeze agent and the balance of deionized water.
According to an embodiment of the invention, the microcapsule suspension-suspension agent comprises the following components in weight percentage: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 12 percent of high molecular capsule wall material, 1 to 12 percent of dispersing agent, 1 to 8 percent of wetting agent, 1 to 50 percent of organic solvent, 1 to 8 percent of emulsifying agent, 0.01 to 2 percent of defoaming agent, 0.1 to 8 percent of thickening agent, 0.1 to 5 percent of pH regulator, 0.1 to 8 percent of antifreeze agent and the balance of deionized water.
According to an embodiment of the invention, the cream comprises the following components in percentage by weight: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 50 percent of organic solvent, 1 to 30 percent of emulsifier, 1 to 10 percent of antifreeze agent, 0.1 to 5 percent of stabilizer and the balance of organic solvent.
According to an embodiment of the invention, the microemulsion comprises the following components in weight percent: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 50 percent of organic solvent, 1 to 30 percent of emulsifier, 1 to 10 percent of antifreeze agent, 0.1 to 5 percent of stabilizer and the balance of deionized water.
According to an embodiment of the invention, the dispersible agent comprises the following components in weight percent: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 30 percent of emulsifier, 1 to 10 percent of antifreeze agent, 0.1 to 5 percent of stabilizer and the balance of organic solvent.
According to an embodiment of the invention, the granule comprises the following components in percentage by weight: 0.5 to 10 percent of zoxamide, 0.5 to 10 percent of fludioxonil, 1 to 12 percent of dispersing agent, 1 to 10 percent of wetting agent, 0.1 to 8 percent of adhesive and the balance of filler.
According to an embodiment of the present invention, the emulsifier may be selected from one or more of sodium lignin sulfonate, agricultural milk, phenyl phenol polyoxyethylene ether phosphate, tristyrylphenol polyoxyethylene ether phosphate triethanolamine salt, benzyl dimethyl phenol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, sorbitan oleate (span-80), fatty alcohol polyoxyethylene ether, sodium alkyl naphthalene sulfonate, sodium isooctyl succinate sulfonate, nonylphenol polyoxyethylene ether phosphate, castor oil polyoxyethylene ether phosphate.
According to an embodiment of the present invention, the dispersant may be selected from one or more of glycerol fatty acid polyoxyethylene ether, polyoxyethylene alkylaryl ether, sodium lignin sulfonate, naphthalene sulfonate formaldehyde condensate, fatty alcohol polyoxyethylene ether sulfate, naphthalene sulfonate formaldehyde condensate sodium salt, nonylphenol polyoxyethylene ether, polyoxyethylene lanolin alcohol, alkylphenol polyoxyethylene ether formaldehyde condensate, fatty alcohol polyoxyethylene ether phosphate, polyoxyethylene sorbitan fatty acid ester, phosphate.
According to an embodiment of the present invention, the wetting agent may be selected from one or more of trisiloxane polyoxyethylene ether, sodium N-lauroyl glutamate, sodium dodecyl sulfate, sodium lauroyl sarcosinate, sodium methylnaphthalene sulfonate formaldehyde condensate, castor oil polyoxyethylene ether, triphenyl ethylphenol polyoxyethylene ether, sodium dodecylbenzene sulfonate, sodium alkylnaphthalene sulfonate, sodium isooctyl succinate sulfonate, polyoxyethylene alkylaryl ether, fatty alcohol polyether glycerol fatty acid polyoxyethylene ether, fatty alcohol polyoxyethylene ether.
According to an embodiment of the present invention, the binder may be selected from one or more of xanthan gum, starch, urea-formaldehyde resin, gelatin, acacia, carboxymethyl cellulose, carboxyethyl cellulose, polyvinyl alcohol.
According to an embodiment of the present invention, the disintegrant may be selected from: sodium bicarbonate, ammonium sulfate, sodium sulfate, calcium sulfate, and magnesium chloride.
According to an embodiment of the present invention, the thickener may be selected from one or more of magnesium aluminum silicate, polyvinyl acetate, xanthan gum, gelatin, acacia, polyvinyl alcohol.
According to an embodiment of the present invention, the antifoaming agent may be selected from one or more of silicone oil, n-octanol, silicone, butyl phosphate, isobutyl phosphate, and the like.
According to an embodiment of the present invention, the antifreeze agent may be selected from one or more of propylene glycol, ethylene glycol, glycerol, and the like.
According to an embodiment of the present invention, the stabilizer may be selected from one or more of triethanolamine, epichlorohydrin, butyl glycidyl ether, triphenyl phosphite, N-soybean oil-based trimethylene diamine, dialkyl succinic acid vinegar sulfonate, and the like.
According to an embodiment of the invention, the filler comprises a solid filler and a liquid filler; the solid filler can be one or more selected from kaolin, attapulgite, diatomite, white carbon black, bentonite, montmorillonite, calcium carbonate and talcum powder. The liquid filler may be selected from one or more of soybean oil, castor oil, mineral oil.
According to an embodiment of the present invention, the organic solvent may be selected from one or more of ethyl acetate, acetone, isopropyl alcohol, 2-trifluoroethanol, propylene carbonate, benzene, toluene, xylene, dimethylformamide, dimethyl sulfoxide, dichloromethane, cyclohexane, cyclohexanone, N-methylpyrrolidone, and a solvent oil (e.g., 150# solvent oil).
The invention also provides application of the bactericidal composition in preventing and controlling crop diseases, such as gray mold or epidemic disease of crops. Preferably, the plant extract is applied to the control of gray mold of crops such as flowers, vegetables, rice, wheat, fruit trees, cotton, rape and the like, and the control of epidemic diseases, late blight and the like of crops such as solanaceae, lily family, cucurbitaceae and the like, for example, the control of pepper epidemic diseases and potato late blight.
The bactericidal composition can be provided in a finished preparation form or can be provided in a single-dose form, and can be directly mixed before use, then water is added to be uniformly mixed to prepare the required concentration, and the bactericidal composition can be applied to plants or crops in any way, such as spraying, plant root irrigation, smearing and the like. In particular application, the composition can also be mixed with other medicaments such as pesticides, growth regulators, soil regulators, herbicides, nematicides and the like.
Advantageous effects
The invention provides a novel efficient pesticide sterilization composition comprising zoxamide and fludioxonil. The bactericidal composition obtained by mixing the zoxamide and the fludioxonil has obvious synergy and complementation effects, and the control effect is obviously improved; the two active ingredients have different action mechanisms, and the mixed use can delay the generation of drug resistance of pathogenic bacteria; the application amount is reduced, the use times are reduced, and the use cost is reduced. The bactericidal composition can effectively prevent and treat various diseases on crops such as vegetables, fruit trees and the like, is particularly suitable for preventing and treating gray mold of crops such as flowers, vegetables, rice, wheat, fruit trees, cotton, rape and the like, and preventing and treating epidemic diseases of crops such as Solanaceae, liliaceae, cucurbitaceae and the like, such as pepper epidemic disease, potato late blight and the like.
Detailed Description
The technical scheme of the invention will be further described in detail below with reference to specific embodiments. It is to be understood that the following examples are illustrative only and are not to be construed as limiting the scope of the invention. All techniques implemented based on the above description of the invention are intended to be included within the scope of the invention.
Unless otherwise indicated, the starting materials and reagents used in the following examples were either commercially available or may be prepared by known methods.
The invention adopts a method combining indoor bioassay and field test to perform performance test on the sterilization effect of the sterilization composition.
The embodiment of the invention adopts a method combining indoor toxicity measurement and field test. Firstly, determining the Synergy Ratio (SR) of two medicaments after being compounded according to a certain proportion through indoor toxicity measurement, wherein SR is less than or equal to 0.5 and is antagonistic, SR is less than 0.5 and is less than 1.5 and is additive, SR is more than or equal to 1.5 and is synergistic, and then carrying out field test on the basis.
Inhibition of fungal hypha growth assay-Petri dish method: refer to the national agricultural industry Standard NY/T1156.2-2006 of the people's republic of China. Firstly, 5 different concentration gradients (on the basis of preliminary experiment results, according to different medicament combinations and proportions, the concentration gradients are also different, the bacteriostasis rate is set in the range of 5% -90% according to geometric progression) are set for single agents of zoxamide and fludioxonil, and solvent comparison is set. The method is carried out by referring to pesticide laboratory bioassay test criterion bactericide, and the toxicity of the pesticide to crop pathogens is measured. After the diameter of the control colony is about 7cm, the diameter of the colony is measured by a crisscross method, and the net growth and the hypha growth inhibition rate of each group of treatments are calculated.
Net growth (mm) =measurement colony diameter (mm) -5mm
Conversion of the hypha growth inhibition rate into a mechanical value (y), conversion of the concentration of the drug solution (μg/mL) into a pair number (x), calculation of a virulence regression equation (y=a+bx) by a least square method, and calculation of EC for each drug from this 50 Values. Meanwhile, the combined Synergy Ratio (SR) of the two medicaments in different proportions is calculated according to the Wadley method, and SR is smaller than or equal to 0.5 and smaller than SR, wherein SR is antagonism, and SR is smaller than 0.5<1.5 is additive effect, and SR is more than or equal to 1.5 is synergistic effect. The calculation formula is as follows:
SR＝EC 50 (theoretical value)/EC 50 (actual measurement value)
EC 50 (theoretical value) = (a+b)/(EC of a/a) 50 ) ++ (EC of B/B) 50 )]
Wherein: a is zoxamide, and B is fludioxonil; a. b is the proportion of the zoxamide and fludioxonil in the composition respectively.
1. Indoor toxicity measurement example
Test example 1: indoor combined toxicity determination of zoxamide and fludioxonil on botrytis cinerea which is a source of botrytis cinerea
(1) Test pathogenic bacteria: botrytis cinerea (b.cinerea);
(2) Test agent: are all provided by Shandong United states of biological technology stock Co., ltd;
(3) And (3) test design: and a preliminary test is carried out to determine the effective inhibition concentration range of the mixture of the zoxamide and the fludioxonil in different weight ratios.
Toxicity measurement results:
TABLE 1 determination of Botrytis cinerea toxicity by single and mixed formulations of zoxamide and fludioxonil
As shown in the results of Table 1, when the weight ratio of the zoxamide to the fludioxonil is 1:50-50:1, the inhibition activities on the in-vitro hyphae of the botrytis cinerea are synergistic, the ratio SR is greater than 1.5, the zoxamide and the fludioxonil are mixed within the range of 1:50-50:1 to show synergistic effects, and when the weight ratio of the zoxamide to the fludioxonil is 1:20-20:1, the synergistic effects are more prominent, and the synergistic ratios are greater than 1.9209. Experiments show that the preferable ratio of the zoxamide to the fludioxonil is 1:20-20:1, wherein the synergistic ratio is the largest when the weight ratio of the zoxamide to the fludioxonil is 1:1, and the synergistic effect is the most obvious.
Test example 2: indoor combined toxicity determination of zoxamide and fludioxonil on phytophthora capsici
(1) Test pathogenic bacteria: phytophthora capsici (P.capsici);
(2) Test agent: are all provided by Shandong United states of biological technology stock Co., ltd;
(3) And (3) test design: and a preliminary test is carried out to determine the effective inhibition concentration range of the mixture of the zoxamide and the fludioxonil in different weight ratios.
Toxicity measurement results:
TABLE 2 determination of Phytophthora capsici toxicity by single and mixed dosage of zoxamide and fludioxonil
The results in Table 2 show that when the weight ratio of the zoxamide to the fludioxonil is 1:50-50:1, the inhibition activities on the isolated hyphae of phytophthora capsici are synergistic, the ratio SR is greater than 1.5, the two are mixed within the range of 1:50-50:1 to show synergistic effects, and when the weight ratio of the zoxamide to the fludioxonil is 1:20-20:1, the synergistic effects are more prominent, and the synergistic ratios are greater than 1.9257. Experiments show that the preferable ratio of the zoxamide to the fludioxonil is 1:20-20:1, wherein the synergistic ratio is the largest when the weight ratio of the zoxamide to the fludioxonil is 1:1, and the synergistic effect is the most obvious.
Test example 3: indoor combined virulence determination of zoxamide and fludioxonil on phytophthora infestans
(1) Test pathogenic bacteria: phytophthora infestans (P.infestans);
(2) Test agent: are all provided by Shandong United states of biological technology stock Co., ltd;
(3) And (3) test design: and a preliminary test is carried out to determine the effective inhibition concentration range of the mixture of the zoxamide and the fludioxonil in different weight ratios.
Toxicity measurement results:
TABLE 3 determination of the toxicity of zoxamide and fludioxonil alone and in combination to Phytophthora infestans
The results in Table 3 show that when the weight ratio of the zoxamide to the fludioxonil is 1:50-50:1, the inhibition activities on the in-vitro mycelia of phytophthora infestans are synergistic, the ratio SR is greater than 1.5, the two are mixed within the range of 1:50-50:1 to show synergistic effects, and when the weight ratio of the zoxamide to the fludioxonil is 1:20-20:1, the synergistic effects are more prominent, and the synergistic ratios are greater than 1.9438. Experiments show that the preferable ratio of the zoxamide to the fludioxonil is 1:20-20:1, wherein the synergistic ratio is the largest when the weight ratio of the zoxamide to the fludioxonil is 1:1, and the synergistic effect is the most obvious.
2. Formulation examples
Example 1:40% zoxamide-fludioxonil wettable powder
The materials are coarsely crushed according to the proportion, then are mixed uniformly in a mixer, and are crushed by air flow to obtain the finished product.
Comparative example 1.1:40% zoxamide wettable powder
Other operations were carried out as in example 1, except that 40% of zoxamide was added and fludioxonil was not added.
Comparative example 1.2:40% fludioxonil wettable powder
Other operations were carried out as in example 1, except that 40% fludioxonil was added and that zoxamide was not added.
Example 2:30% zoxamide-fludioxonil water dispersible granule
Uniformly mixing the effective active ingredients, various auxiliary agents and the like according to the proportion of the formula, obtaining powder after jet milling, adding water accounting for 15% of the total mass of the mixture, mixing, extruding and granulating, and obtaining a finished product after drying and screening.
Comparative example 2.1:30% zoxamide water dispersible granule
Other operations were carried out as in example 2, except that 30% of zoxamide was added and fludioxonil was not added.
Comparative example 2.2:30% fludioxonil water dispersible granule
Other operations were carried out as in example 2, except that 30% fludioxonil was added and that zoxamide was not added.
Example 3:20% zoxamide-fludioxonil suspension
The effective active ingredients and various auxiliary agents are uniformly mixed according to the proportion of the formula, sheared at high speed and sanded to obtain the finished product.
Comparative example 3.1:20% zoxamide suspending agent
Other operations were carried out as in example 3, except that 20% of zoxamide was added and fludioxonil was not added.
Comparative example 3.2:20% fludioxonil suspension
Other operations were carried out as in example 3, except that 20% fludioxonil was added and that zoxamide was not added.
Example 4:50% zoxamide-fludioxonil emulsifiable concentrate
Mixing the raw materials in proportion to dissolve the raw materials into a uniform oil phase; and (5) after the medicament content, the pH range, the stability and the like are checked to be qualified, metering and split charging are carried out, and the finished product is obtained.
Comparative example 4.1:50% zoxamide emulsifiable concentrate
The other operations are the same as in example 4, except that 50% zoxamide is added and fludioxonil is not added.
Comparative example 4.2:50% fludioxonil emulsifiable concentrate
The other procedure is as in example 4, except that 50% fludioxonil is added and zoxamide is not added.
Example 5:25% zoxamide-fludioxonil aqueous emulsion
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According to the formula requirement, the raw materials are added into a batching kettle, uniformly mixed by a high-speed shearing machine to prepare the aqueous emulsion, and after the medicament content, the pH range, the stability and the like are checked to be qualified, the metering and the sub-packaging are carried out to obtain the finished product.
Comparative example 5.1:25% zoxamide aqueous emulsion
Other operations were carried out as in example 5, except that 25% of zoxamide was added and fludioxonil was not added.
Comparative example 5.2:25% fludioxonil water emulsion
Other operations were carried out as in example 5, except that 25% fludioxonil was added and that zoxamide was not added.
The field control effect test implementation method comprises the following steps: the single component preparation is used as a control medicament of the compound preparation, gray mold is used as a target, and the control effects on gray mold and epidemic disease of different crops are respectively measured in 5 groups of examples. For example, 40% zoxamide suspension and 40% fludioxonil suspension are used as control agents for 40% zoxamide fludioxonil suspension (example 1), and so on. The test is carried out according to the method of drug administration prescribed in national Standard of the people's republic of China GB/T17980.28-2000, drug administration is carried out when the disease spots or the disease fruits are seen at first, the drug administration is carried out 2 nd time after 7 days, the drug administration is carried out twice in total, and the times of repetition are 4 times. The disease pre-drug base numbers of different crops are investigated before the drug is applied, the control effect is investigated according to the disease condition after the drug is applied, the drug is applied by adopting a spraying method, the disease condition is recorded according to national standard or row standard grading standard, and the control effect is calculated.
Table 4 field efficacy test of zoxamide and fludioxonil compounded on cucumber gray mold
Note that: the different letters after the same column of data represent significant differences at P <0.05 level as tested by Duncan's new complex polar error method, the same applies below.
From table 4, it can be seen that after the zoxamide and fludioxonil are compounded, the cucumber gray mold can be effectively prevented and treated, and the preventing and treating effects are obviously better than those of single dosage, which indicates that the compound of the zoxamide and fludioxonil can improve the preventing and treating effects, reduce the dosage of pesticides, reduce the influence of pesticides on the environment and reduce the cost of pesticides. And has no adverse effect on target crops in the experimental application range.
Table 5 field efficacy test of zoxamide and fludioxonil compounded on strawberry gray mold
From table 5, it can be seen that the compound of the zoxamide and the fludioxonil can effectively prevent and treat the gray mold of the strawberry, and the preventing and treating effects are obviously better than those of single dosage, which indicates that the compound of the zoxamide and the fludioxonil can improve the preventing and treating effects, reduce the dosage of pesticides, reduce the influence of the pesticides on the environment and reduce the cost of the pesticides. And has no adverse effect on target crops in the experimental application range.
Table 6 zoxamide and fludioxonil compounded field efficacy test against pepper epidemic disease
As can be seen from table 6, the compound of the zoxamide and the fludioxonil can effectively prevent and treat the pepper epidemic disease, and the preventing and treating effects are obviously better than those of single dosage, which indicates that the compound of the zoxamide and the fludioxonil can improve the preventing and treating effects, reduce the dosage of pesticides, reduce the influence of the pesticides on the environment and reduce the cost of the pesticides. And has no adverse effect on target crops in the experimental application range.
Table 7 zoxamide and fludioxonil compounded potato late blight field efficacy test
As can be seen from table 7, after the zoxamide and fludioxonil are compounded, the potato late blight can be effectively prevented and treated, and the prevention and treatment effects are obviously better than those of a single dose, which indicates that the zoxamide and fludioxonil compounded can improve the prevention and treatment effects, reduce the dosage of pesticides, reduce the influence of the pesticides on the environment and reduce the medication cost. And has no adverse effect on target crops in the experimental application range.
The embodiments of the present invention have been described above. However, the present invention is not limited to the above embodiment. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (14)
1. A bactericidal composition comprising the following components: zoxamide, fludioxonil and auxiliary materials; the active ingredients of the composition are zoxamide and fludioxonil, and the weight ratio of the zoxamide to the fludioxonil is (1-50) (50-1);
the sum of the weight percentages of the zoxamide and the fludioxonil is 1-90% based on the total weight of the bactericidal composition.
2. The fungicidal composition of claim 1, wherein the weight ratio of zoxamide to fludioxonil is (1-40): 40-1.
3. The fungicidal composition of claim 1, wherein the weight ratio of zoxamide to fludioxonil is (1-30): 30-1.
4. The bactericidal composition of claim 1, wherein the weight ratio of zoxamide to fludioxonil is (1-20): 20-1.
5. The bactericidal composition of claim 1, wherein the sum of the weight percentages of zoxamide and fludioxonil is 10 to 80%, based on the total weight of the bactericidal composition.
6. The bactericidal composition of claim 1, wherein the sum of the weight percentages of zoxamide and fludioxonil is 15 to 70%, based on the total weight of the bactericidal composition.
7. The bactericidal composition of claim 1, wherein the sum of the weight percentages of zoxamide and fludioxonil is 20 to 60%, based on the total weight of the bactericidal composition.
8. The bactericidal composition of claim 1, wherein the bactericidal composition is a liquid formulation or a solid formulation.
9. The bactericidal composition of claim 8, wherein the sum of the weight percentages of zoxamide and fludioxonil in the liquid formulation is 1% to 60%;
in the solid preparation, the sum of the weight percentages of the zoxamide and the fludioxonil is 5-80%.
10. The bactericidal composition of claim 8, wherein the sum of the weight percentages of zoxamide and fludioxonil in the liquid formulation is between 5% and 50%;
in the solid preparation, the sum of the weight percentages of the zoxamide and the fludioxonil is 10-70%.
11. A bactericidal composition according to claim 1,
when the bactericidal composition is a suspending agent, the bactericidal composition comprises the following components in percentage by weight: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 12 percent of dispersing agent, 1 to 10 percent of wetting agent, 0.1 to 8 percent of thickening agent, 0.1 to 8 percent of antifreeze agent and the balance of deionized water;
when the bactericidal composition is an aqueous emulsion, the bactericidal composition comprises the following components in percentage by weight: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 60 percent of organic solvent, 1 to 12 percent of emulsifier, 0.1 to 8 percent of antifreeze agent, 0.01 to 2 percent of defoamer, 0.1 to 2 percent of thickener and the balance of deionized water;
when the bactericidal composition is a suspended seed coating agent, the bactericidal composition comprises the following components in percentage by weight: 1-50% of zoxamide, 1-50% of fludioxonil, 1-12% of dispersing agent, 1-10% of wetting agent, 1-10% of antifreeze agent, 0.1-10% of anticaking agent, 0.1-5% of suspending agent, 1-10% of film forming agent, 0.1-5% of preservative, 1-30% of colorant, 0.1-5% of pH regulator, 0.1-8% of thickener and the balance of deionized water;
when the bactericidal composition is a microcapsule suspending agent, the bactericidal composition comprises the following components in percentage by weight: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 30 percent of high molecular capsule wall material, 2 to 10 percent of dispersing agent, 1 to 50 percent of organic solvent, 1 to 7 percent of emulsifying agent, 0.1 to 5 percent of pH regulator, 0.01 to 2 percent of defoaming agent, 0.1 to 8 percent of thickening agent, 0.1 to 8 percent of antifreeze agent and the balance of deionized water;
when the bactericidal composition is a microcapsule suspension-suspending agent, the bactericidal composition comprises the following components in percentage by weight: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 12 percent of high molecular capsule wall material, 1 to 12 percent of dispersing agent, 1 to 8 percent of wetting agent, 1 to 50 percent of organic solvent, 1 to 8 percent of emulsifying agent, 0.01 to 2 percent of defoaming agent, 0.1 to 8 percent of thickening agent, 0.1 to 5 percent of pH regulator, 0.1 to 8 percent of antifreeze agent and the balance of deionized water;
when the bactericidal composition is missible oil, the bactericidal composition comprises the following components in percentage by weight: 1 to 50 percent of zoxamide, 1 to 50 percent of fludioxonil, 1 to 50 percent of organic solvent, 1 to 30 percent of emulsifier, 1 to 10 percent of antifreeze agent, 0.1 to 5 percent of stabilizer and the balance of organic solvent;
when the bactericidal composition is a microemulsion, the bactericidal composition comprises the following components in percentage by weight: 1-50% of zoxamide, 1-50% of fludioxonil, 1-50% of organic solvent, 1-30% of emulsifier, 1-10% of antifreeze agent, 0.1-5% of stabilizer and the balance of deionized water;
when the bactericidal composition is a dispersible agent, the bactericidal composition comprises the following components in percentage by weight: 1-50% of zoxamide, 1-50% of fludioxonil, 1-30% of emulsifier, 1-10% of antifreeze agent, 0.1-5% of stabilizer and the balance of organic solvent;
when the bactericidal composition is granule, the bactericidal composition comprises the following components in percentage by weight: 0.5 to 10 percent of zoxamide, 0.5 to 10 percent of fludioxonil, 1 to 12 percent of dispersing agent, 1 to 10 percent of wetting agent, 0.1 to 8 percent of adhesive and the balance of filler.
12. The method for preparing the bactericidal composition as set forth in any one of claims 1 to 11, comprising the step of mixing zoxamide, fludioxonil and an auxiliary material to obtain the bactericidal composition.
13. Use of the fungicidal composition according to any one of claims 1-11 for controlling crop gray mold or epidemic disease caused by Botrytis cinerea (Botrytis cinerea), phytophthora capsici (Phytophthora capsici) or phytophthora infestans (Phytophthora infestans).
14. Use according to claim 13, of a bactericidal composition according to any of claims 1-11 for controlling botrytis cinerea, pepper blight, potato late blight.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1444449A (en) * | 2000-07-04 | 2003-09-24 | 辛根塔参与股份公司 | Fungicidal compositions based on fludioxonil |
| CN101653118A (en) * | 2009-07-31 | 2010-02-24 | 深圳诺普信农化股份有限公司 | Compound synergistic bactericidal composition |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1444449A (en) * | 2000-07-04 | 2003-09-24 | 辛根塔参与股份公司 | Fungicidal compositions based on fludioxonil |
| CN101653118A (en) * | 2009-07-31 | 2010-02-24 | 深圳诺普信农化股份有限公司 | Compound synergistic bactericidal composition |
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