CN114835719A

CN114835719A - Substituted bicyclic aromatic heterocyclic amine inhibitor and preparation method and application thereof

Info

Publication number: CN114835719A
Application number: CN202110138300.6A
Authority: CN
Inventors: 吕彬华; 崔大为
Original assignee: Suzhou Zelgen Biopharmaceutical Co Ltd; Shanghai Zelgen Pharmatech Co Ltd
Current assignee: Suzhou Zelgen Biopharmaceutical Co Ltd; Shanghai Zelgen Pharmatech Co Ltd
Priority date: 2021-02-01
Filing date: 2021-02-01
Publication date: 2022-08-02
Also published as: WO2022161480A1

Abstract

The invention relates to a substituted bicyclic aromatic heterocyclic amine inhibitor and a preparation method and application thereof. The compound has a structure shown in a formula (I), and also discloses a preparation method of the compound and application of the compound as an SOS1 inhibitor, and the compound has a good selective inhibition effect on SOS1, better pharmacodynamics and pharmacokinetic properties and lower toxic and side effects.

Description

Substituted bicyclic aromatic heterocyclic amine inhibitor and preparation method and application thereof

Technical Field

The invention belongs to the field of medicines, and particularly relates to a substituted bicyclic aromatic heterocyclic amine inhibitor, and a preparation method and application thereof.

Background

Lung cancer is one of the leading causes of death in human cancers. Lung cancer can be divided into Small Cell Lung Cancer (SCLC) and non-small cell lung cancer (NSCLC) according to cell type, with NSCLC accounting for 85% of all lung cancer patients. The global NSCLC market was statistically estimated to be $ 209 billion in 2016, with the U.S. market taking up half, followed by Japan, Germany, and China. From the current trend, the market for non-small cell lung cancer is continuing to grow, and the global market is expected to reach $ 540 billion in 2023 (Nature, 2018; 553(7689):446- > 454).

At present, the main therapeutic drugs for NSCLC include chemotherapeutic drugs, molecular targeted drugs, tumor immunotherapy and the like. The chemotherapy drugs mainly comprise gemcitabine, paclitaxel, platinum drugs and the like, but the drugs generally have poor selectivity and high toxicity, so that relatively strong toxic and side effects are caused. In recent years, molecular targeted drugs have become research hotspots due to the obvious advantages of high selectivity, relatively small toxic and side effects, accurate treatment and the like. Existing NSCLC molecular targeted drugs include EGFR inhibitors (e.g., Afatinib, Gefitinib, Erlotinib, Lapatinib, Dacomitinib, Icotinib, Pyrotinib, Rociletinib, Osimertinib, etc.), ALK inhibitors (e.g., Ceritinib, aletinib, Brigatinib, loretinib, ocatinib, etc.), and VEGFR inhibitors (Sorafenib, regrafenib, Cabozantinib, Sunitinib, doranib, etc.) (Current Medicinal Chemistry,2019,26, 1-39).

KRAS mutations occur in 20-40% of lung adenocarcinomas, with a higher prevalence in western (vs asia) populations (26% vs 11%) and in smokers (vs non-smokers) (30% vs 10%). The most common mutations occur in codons 12 and 13, the most common mutations include G12C, G12V, and G12D. To date, no drug against KRAS mutations has been approved for marketing in the market.

Within the cell, the KRAS protein transitions between an inactive and an active state, when KRAS is bound to Guanosine Diphosphate (GDP), it is in the inactive state, when it is bound to Guanosine Triphosphate (GTP), it is in the active state, and downstream signaling pathways can be activated. The transition of KRAS between inactive and active states is regulated by two types of factors. One class is guanine nucleotide exchange factor (GEF), which catalyzes the binding of KRAS to GTP, thereby promoting the activation of KRAS, including the SOS1 protein. Another class is the Gtpase Activating Proteins (GAPs), which promote the hydrolysis of KRAS-bound GTP to GDP, thereby inhibiting KRAS activity.

To date, three major RAS-specific GEFs have been identified, with SOS proteins being primarily found in tumors. SOS proteins are widely expressed in vivo and contain two isoforms SOS1 and SOS 2. Published data indicate that SOS1 plays a key role in mutant KRAS activation and oncogenic signaling. A decrease in the level of SOS1 resulted in a decrease in the proliferation and survival of tumor cells carrying the KRAS mutation, whereas KRAS wild-type cell lines were not affected. The effect of the loss of SOS1 could not be rescued by introducing a SOS1 mutated at the catalytic site, suggesting an important role for SOS1 GEF activity in KRAS mutant cancer cells (see WO2019122129a 1).

Since KRAS binding to GTP, whether mutated or wild-type, is dependent on SOS1, selective inhibition of SOS1, whether mutated or not, prevents interaction of SOS1 with KRAS and ultimately inhibits KRAS activation.

Since the SOS1 target protein is pathologically associated with a variety of diseases, there is also a need for novel SOS1 inhibitors for clinical therapy. The SOS1 inhibitor with high selectivity and high activity can be used for more effectively treating diseases such as cancers caused by KRAS mutation and reducing the potential of off-target effect, so that the clinical demand is more urgent.

Disclosure of Invention

The invention aims to provide a novel compound with selective inhibition effect on SOS1 and/or better pharmacodynamic property and application thereof.

In a first aspect of the invention, there is provided a compound of formula (I), a stereoisomer, a tautomer, a crystalline form, a pharmaceutically acceptable salt, a hydrate, a solvate, or a prodrug thereof:

in the formula:

the same or different, each independently a single bond or a double bond;

x, Y, U and V are each independently selected from: C. CR ^x Or N, wherein R ^x Selected from: H. d, CN or halogen;

ring a is selected from the group consisting of substituted or unsubstituted: phenyl, 5-6 membered heteroaryl, C ₄ -C ₆ Cycloalkyl and 4-6 membered heterocyclyl;

ring B is selected from the group consisting of substituted or unsubstituted: c ₃ -C ₂₀ Cycloalkyl, 4-20 membered heterocyclyl, C ₆ -C ₁₄ Aryl or 5-14 membered heteroaryl;

R ¹ 、R ² the same or different, each independently selected from the group consisting of: hydrogen, deuterium, halogen, cyano, oxo, substituted or unsubstituted C ₂ -C ₁₈ Alkenyl, substituted or unsubstituted C ₂ -C ₁₈ Alkynyl, substituted or unsubstituted C ₁ -C ₁₈ Alkyl, substituted or unsubstituted C ₃ -C ₂₀ Cycloalkyl, substituted or unsubstituted 4-20 membered heterocycloalkyl, substituted or unsubstituted C ₁ -C ₁₈ Alkylene radical C ₃ -C ₂₀ Cycloalkylene, substituted or unsubstituted C ₁ -C ₁₈ Alkylene 4-20 membered heterocycloalkylene, - (CH) ₂ ) _h O(CH ₂ ) _p R ⁶ 、-(CH ₂ ) _h SR ⁶ 、-(CH ₂ ) _h COR ⁶ 、-(CH ₂ ) _h C(O)OR ⁶ 、-(CH ₂ ) _h S(O) _q R ⁶ 、-(CH ₂ ) _h NR ⁶ R ⁷ 、-(CH ₂ ) _h C(O)NR ⁶ R ⁷ 、-(CH ₂ ) _h NR ⁶ C(O)R ⁷ 、-(CH ₂ ) _h NR ⁶ C(O)NR ⁷ R ⁸ 、-(CH ₂ ) _h S(O) _q NR ⁶ R ⁷ 、-(CH ₂ ) _h NR ⁶ S(O) _q R ⁷ 、-(CH ₂ ) _h NR ⁶ S(O) _q NR ⁷ R ⁸ (ii) a Wherein, CH ₂ H in (a) may be optionally substituted; r ⁶ 、R ⁷ And R ⁸ Each independently selected from the group consisting of: hydrogen, substituted or unsubstituted C ₁ -C ₁₈ Alkyl, substituted or unsubstituted C ₃ -C ₂₀ Cycloalkyl, substituted or unsubstituted 4-20 membered heterocyclyl, substituted or unsubstituted C ₆ -C ₁₄ Aryl and substituted or unsubstituted 5-14 membered heteroaryl; or in- (CH) ₂ ) _h NR ⁶ R ⁷ 、-(CH ₂ ) _h C(O)NR ⁶ R ⁷ 、-(CH ₂ ) _h S(O) _q NR ⁶ R ⁷ In, R ⁶ And R ⁷ And the adjacent N atom thereof form a substituted or unsubstituted 4-8 membered heterocyclic group; or in- (CH) ₂ ) _h NR ⁶ C(O)R ⁷ 、-(CH ₂ ) _h NR ⁶ C(O)NR ⁷ R ⁸ 、-(CH ₂ ) _h NR ⁶ S(O) _q R ⁷ 、-(CH ₂ ) _h NR ⁶ S(O) _q NR ⁷ R ⁸ In, R ⁷ And R ⁸ Is cyclized with its adjacent N atom to form a substituted or unsubstituted 4-8 membered heterocyclic group, or R ⁶ And R ⁷ Ring-closing with its adjacent atom to form a substituted or unsubstituted 4-8 membered heterocyclic group;

R ³ selected from the group consisting of substituted or unsubstituted: c ₃ -C ₁₈ Cycloalkyl, 4-20 membered heterocyclyl, C ₆ -C ₁₄ Aryl and 5-14 membered heteroaryl;

R ⁴ 、R ⁵ independently selected from the group consisting of substituted or unsubstituted: c ₁ -C ₆ Alkyl radical, C ₃ -C ₆ Cycloalkyl and 4-6 membered heterocyclyl;

wherein, the ring A and the ring B, R ¹ 、R ² 、R ³ 、R ⁴ 、R ⁵ 、R ⁶ 、R ⁷ And R ⁸ Substituted means substituted with one or more groups selected from the group consisting of: hydrogen, deuterium, C ₁ -C ₁₈ Alkyl, deuterated C ₁ -C ₁₈ Alkyl, halo C ₁ -C ₁₈ Alkyl, halo C ₁ -C ₁₈ Alkyl hydroxy, C ₃ -C ₂₀ Cycloalkyl radical, C ₁ -C ₁₈ Alkoxy, deuterated C ₁ -C ₁₈ Alkoxy, halo C ₁ -C ₁₈ Alkoxy radical, C ₆ -C ₁₄ Aryl, 5-14 membered heteroaryl, 4-20 membered heterocyclyl, halogen, nitro, hydroxy, oxo, cyano, ester, amine, amide, sulfonamide, and urea;

m is 0,1, 2,3 or 4;

n is 0,1, 2,3, 4 or 5;

h is 0,1, 2,3, 4,5 or 6;

p is 0,1, 2,3, 4,5 or 6;

q is 1 or 2.

In a further preferred embodiment of the method,

the moiety is a substituted or unsubstituted fused, bridged or spiro ring structure.

In addition toIn a preferred embodiment, in formula I,

moieties are selected from:

in another preferred embodiment, in formula I, R ⁴ And R ⁵ Each independently selected from: halogen, C ₁ -C ₃ Alkyl, preferably, R ⁴ And R ⁵ Is methyl.

In another preferred embodiment, in formula I,

is partially made of

Wherein ring A and ring B are as defined above.

In another preferred embodiment, in formula I,

is partially made of

Wherein W is selected from: n or CR ^y ；R ^y Selected from the group consisting of: hydrogen, deuterium, halogen, cyano, substituted or unsubstituted C ₁ -C ₃ Alkyl, substituted or unsubstituted 3-6 membered cycloalkyl and substituted or unsubstituted 4-6 membered heterocycloalkyl;

wherein the above substitution means substitution with one or more groups selected from the group consisting of: hydrogen, deuterium, C ₁ -C ₁₈ Alkyl, deuterated C ₁ -C ₁₈ Alkyl, halo C ₁ -C ₁₈ Alkyl, halo C ₁ -C ₁₈ Alkyl hydroxy, C ₃ -C ₂₀ Cycloalkyl, C ₁ -C ₁₈ Alkoxy, deuterated C ₁ -C ₁₈ Alkoxy, haloC ₁ -C ₁₈ Alkoxy radical, C ₆ -C ₁₄ Aryl, 5-14 membered heteroaryl, 4-20 membered heterocyclyl, halogen, nitro, hydroxy, cyano, ester, amine, amide, sulfonamide, or ureido;

ring B is as defined above.

In another preferred embodiment, the compound, its stereoisomer, tautomer, crystal form, pharmaceutically acceptable salt, hydrate, solvate or prodrug has the structure shown in the formula (II):

wherein, ring A, ring B, R ¹ 、R ² 、R ³ 、R ⁴ 、R ⁵ M and n are as defined above.

In another preferred embodiment, the compound, its stereoisomer, tautomer, crystal form, pharmaceutically acceptable salt, hydrate, solvate or prodrug has the structure shown in the formula (III):

wherein, ring A, ring B, R ¹ 、R ² 、R ³ 、R ⁴ M and n are as defined above.

In another preferred embodiment, the compound, its stereoisomer, tautomer, crystal form, pharmaceutically acceptable salt, hydrate, solvate or prodrug has the structure shown in the formula (IV):

in the formula: r ¹ 、R ² 、R ³ Ring a, ring B, m, and n are as defined above.

In another preferred embodiment, in the formulae II to IV,

is partially made of

Wherein W and Ring B are as defined above.

In another preferred embodiment, in formula I,

moieties are selected from:

ring C is selected from the group consisting of substituted or unsubstituted: c ₃ -C ₈ Cycloalkyl, 4-8 membered heterocyclyl, C ₆ -C ₁₀ Aryl, 5-10 membered heteroaryl;

X ₁ 、X ₂ each independently selected from: o, CR ⁹ R ¹⁰ 、NR ¹¹ ；X ₃ Selected from: n or CR ⁹ ；R ⁹ 、R ¹⁰ Each independently selected from the group consisting of substituted or unsubstituted: hydrogen, deuterium, C ₁ -C ₆ Alkyl, deuterated C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl hydroxy, C ₃ -C ₁₀ Cycloalkyl radical, C ₁ -C ₆ Alkoxy, deuterated C ₁ -C ₆ Alkoxy, halo C ₁ -C ₆ Alkoxy radical, C ₆ -C ₁₄ Aryl, 5-14 membered heteroaryl, 4-10 membered heterocyclyl, halogen, nitro, hydroxy, oxo, cyano, ester, amine, amide, sulfonamide, or urea; r is ¹¹ Selected from the group consisting of substituted or unsubstituted: hydrogen, deuterium, C ₁ -C ₆ Alkyl, deuterated C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl hydroxy, C ₃ -C ₁₀ Cycloalkyl radical, C ₁ -C ₆ Alkoxy, deuterated C ₁ -C ₆ Alkoxy, halo C ₁ -C ₆ Alkoxy radical, C ₆ -C ₁₄ Aryl, 5-14 membered heteroaryl, 4-10 membered heterocyclyl;

wherein, ring C, R ⁹ 、R ¹⁰ 、R ¹¹ Wherein said substitution is by 1,2, 3 or 4 groups selected from the group consisting of: deuterium, C ₁ -C ₆ Alkyl, deuterated C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl hydroxy, C ₃ -C ₁₀ Cycloalkyl radical, C ₁ -C ₆ Alkoxy, deuterated C ₁ -C ₆ Alkoxy, halo C ₁ -C ₆ Alkoxy radical, C ₆ -C ₁₄ Aryl, 5-14 membered heteroaryl, 4-10 membered heterocyclyl, halogen, nitro, hydroxy, oxo, cyano, ester, amine, amide, sulfonamide, or urea;

n1 is 1,2 or 3;

n2 is 0,1 or 2;

n3 is 1,2, 3 or 4.

In another preferred embodiment, ring C is selected from the group consisting of substituted or unsubstituted: c ₅ -C ₆ Cycloalkyl, 5-6 membered heterocyclyl, phenyl, 5-6 membered heteroaryl, wherein said substitution means substitution by 1,2, 3 or 4 groups selected from: deuterium, C ₁ -C ₆ Alkyl, deuterated C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl hydroxy, C ₃ -C ₁₀ Cycloalkyl, C ₁ -C ₆ Alkoxy, deuterated C ₁ -C ₆ Alkoxy, halo C ₁ -C ₆ Alkoxy radical, C ₆ -C ₁₄ Aryl, 5-14 membered heteroaryl, 4-10 membered heterocyclyl, halogen, nitro, hydroxy, oxo, cyano, ester, amine, amide, sulfonamide, or urea.

In another preferred embodiment, in the formulae II to IV,

moieties are selected from:

wherein, ring C, X ₁ 、X ₂ 、X ₃ N1, n2, n3 are as defined above.

In another preferred embodiment, the compound, its stereoisomer, tautomer, crystal form, pharmaceutically acceptable salt, hydrate, solvate or prodrug has the structure shown in the formula (V):

in the formula:

w is selected from: n or CR ^y ；R ^y Selected from the group consisting of: hydrogen, deuterium, halogen, cyano, substituted or unsubstituted C ₁ -C ₃ Alkyl, substituted or unsubstituted 3-6 membered cycloalkyl and substituted or unsubstituted 4-6 membered heterocycloalkyl;

wherein the above substitution means substitution with one or more groups selected from the group consisting of: hydrogen, deuterium, C ₁ -C ₁₈ Alkyl, deuterated C ₁ -C ₁₈ Alkyl, halo C ₁ -C ₁₈ Alkyl, halo C ₁ -C ₁₈ Alkyl hydroxy, C ₃ -C ₂₀ Cycloalkyl radical, C ₁ -C ₁₈ Alkoxy, deuterated C ₁ -C ₁₈ Alkoxy, halo C ₁ -C ₁₈ Alkoxy radical, C ₆ -C ₁₄ Aryl, 5-14 membered heteroaryl, 4-20 membered heterocyclyl, halogen, nitro, hydroxyl, cyano, ester, amine, amide, sulfonamide, or urea;

R ¹ 、R ² 、R ³ ring B, m and n are as defined above.

In another preferred embodiment, the compound, its stereoisomer, tautomer, crystal form, pharmaceutically acceptable salt, hydrate, solvate or prodrug has the structure shown in the formula (VI):

in the formula (I), the compound is shown in the specification,

R ¹ 、R ² 、R ³ ring B, m, n and R ^y Is as defined above.

In another preferred embodiment, in formulas I-VI, R ¹ 、R ² Each independently selected from the group consisting of: hydrogen, deuterium, halogen, cyano, oxo, substituted or unsubstituted C ₂ -C ₄ Alkenyl, substituted or unsubstituted C ₂ -C ₄ Alkynyl, substituted or unsubstituted C ₁ -C ₆ Alkyl, substituted or unsubstituted C ₃ -C ₆ Cycloalkyl, substituted or unsubstituted 4-6 membered heterocycloalkyl, substituted or unsubstituted C ₁ -C ₆ Alkylene radical C ₃ -C ₁₃ Cycloalkylene, substituted or unsubstituted C ₁ -C ₆ Alkylene 4-12 membered heterocycloalkylene, - (CH) ₂ ) _h O(CH ₂ ) _p R ⁶ 、-(CH ₂ ) _h SR ⁶ 、-(CH ₂ ) _h COR ⁶ 、-(CH ₂ ) _h C(O)OR ⁶ 、-(CH ₂ ) _h S(O) _q R ⁶ 、-(CH ₂ ) _h NR ⁶ R ⁷ 、-(CH ₂ ) _h C(O)NR ⁶ R ⁷ 、-(CH ₂ ) _h NR ⁶ C(O)R ⁷ 、-(CH ₂ ) _h NR ⁶ C(O)NR ⁷ R ⁸ 、-(CH ₂ ) _h S(O) _q NR ⁶ R ⁷ 、-(CH ₂ ) _h NR ⁶ S(O) _q R ⁷ 、-(CH ₂ ) _h NR ⁶ S(O) _q NR ⁷ R ⁸ ；R ⁶ 、R ⁷ And R ⁸ Each independently selected from the group consisting of: hydrogen, substituted or unsubstituted C ₁ -C ₆ Alkyl, substituted or unsubstituted C ₃ -C ₆ Cycloalkyl, substituted or unsubstituted 4-6 membered heterocyclyl, substituted or unsubstituted C ₆ -C ₁₄ Aryl and substituted or unsubstituted 5-14 membered heteroaryl; or in- (CH) ₂ ) _h NR ⁶ R ⁷ 、-(CH ₂ ) _h C(O)NR ⁶ R ⁷ 、-(CH ₂ ) _h S(O) _q NR ⁶ R ⁷ In, R ⁶ And R ⁷ And the adjacent N atom thereof form a substituted or unsubstituted 4-8 membered heterocyclic group; or in- (CH) ₂ ) _h NR ⁶ C(O)R ⁷ 、-(CH ₂ ) _h NR ⁶ C(O)NR ⁷ R ⁸ 、-(CH ₂ ) _h NR ⁶ S(O) _q R ⁷ 、-(CH ₂ ) _h NR ⁶ S(O) _q NR ⁷ R ⁸ In, R ⁷ And R ⁸ Is cyclized with its adjacent N atom to form a substituted or unsubstituted 4-8 membered heterocyclic group, or R ⁶ And R ⁷ Ring-closing with its adjacent atom to form a substituted or unsubstituted 4-8 membered heterocyclic group;

h is 0,1, 2,3, 4,5 or 6;

wherein R is ¹ 、R ² 、R ⁶ 、R ⁷ And R ⁸ Substituted means substituted with one or more groups selected from the group consisting of: hydrogen, deuterium, C ₁ -C ₆ Alkyl, deuterated C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl hydroxy, C ₃ -C ₆ Cycloalkyl radical, C ₁ -C ₆ Alkoxy, deuterated C ₁ -C ₆ Alkoxy, halo C ₁ -C ₆ Alkoxy radical, C ₆ -C ₁₄ Aryl, 5-14 membered heteroaryl, 4-6 membered heterocyclyl, halogen, nitro, hydroxy, oxo, cyano, ester, amine, amide, sulfonamide, and urea.

In another preferred embodiment, in formulas I-VI, R ³ Selected from the group consisting of substituted or unsubstituted: phenyl and 5-6 membered heteroaryl, wherein said substitution means substitution by one or more groups selected from the group consisting of: c ₁ -C ₆ Alkyl, deuterated C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl hydroxy, C ₃ -C ₆ Cycloalkyl radical, C ₁ -C ₆ Alkoxy, deuterated C ₁ -C ₆ Alkoxy, halo C ₁ -C ₆ Alkoxy, halogen, nitro, hydroxyl, oxo, cyano, ester, amine, amide, sulfonamide, or urea.

In another preferred embodiment, the compound, its stereoisomer, tautomer, crystal form, pharmaceutically acceptable salt, hydrate, solvate or prodrug thereof, wherein ring B is selected from the group consisting of substituted or unsubstituted: c ₃ -C ₁₀ Cycloalkyl, 4-16 membered heterocyclyl, phenyl and 5-6 membered heteroaryl, wherein said substitution refers to substitution by one or more groups selected from the group consisting of: c ₁ -C ₆ Alkyl, deuterated C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl hydroxy, C ₃ -C ₆ Cycloalkyl radical, C ₁ -C ₆ Alkoxy, deuterated C ₁ -C ₆ Alkoxy, halo C ₁ -C ₆ Alkoxy, halogen, nitro, hydroxyl, oxo, cyano, ester, amine, amide, sulfonamide, or urea.

In another preferred embodiment, X, Y, U, V, R ¹ 、R ² 、R ³ 、R ⁴ 、R ⁵ Ring A, ring B, m, n, W and R ^y Are groups corresponding to the specific compounds in the examples.

In another preferred embodiment, the compound, its stereoisomer, tautomer, crystal form, pharmaceutically acceptable salt, hydrate, solvate or prodrug thereof is selected from the group consisting of:

in another preferred embodiment, the compound is preferably the compound prepared in the examples.

In a second aspect of the present invention, there is provided a process for the preparation of a compound of formula (III), a stereoisomer, a tautomer, a crystalline form, a pharmaceutically acceptable salt, a hydrate, a solvate, or a prodrug thereof, wherein the process comprises the steps of:

method 1

(i) Reacting the compound shown in the formula (X-1) with acetic anhydride in an inert solvent to obtain a compound shown in the formula (X-2);

(ii) in the presence of an oxidizing agent (e.g. H) ₂ O ₂ Etc.) and a base (e.g., sodium hydroxide, etc.) to obtain a compound of formula (X-3);

(iii) reacting the compound of formula (X-3) with an amine (formula X-4) in the presence of a base (e.g., TEA, DIPEA, Py, DMAP, or DBU, etc.) and a condensing agent (e.g., BOP, or PyBOP, etc.) to obtain a compound of formula (III);

method two

(iv) reacting the compound of formula (X-3) with a compound LG-X in the presence of a base (e.g., TEA, DIPEA, Py, DMAP, etc.) to give a compound of formula (X-5)

(v) Reacting the compound of formula (X-5) with an amine of formula (X-4) in the presence of a base (e.g., TEA, DIPEA, Py, or DMAP, etc.) to obtain a compound of formula (III);

in the formula (I), the compound is shown in the specification,

LG is selected from: halogen, OTs, OMs or OTf;

LG-X is selected from: SOCl ₂ 、SO ₂ Cl ₂ 、POCl ₃ 、MsCl、TsCl、TfOH、TMSOTf、Tf ₂ O or Ms ₂ O and the like;

R ¹ 、R ² 、R ³ x, ring B, m and n are as defined above.

In a third aspect of the invention, there is provided a pharmaceutical composition comprising i) one or more compounds of the first aspect, stereoisomers, tautomers, crystalline forms, pharmaceutically acceptable salts, hydrates, solvates or prodrugs thereof; and ii) a pharmaceutically acceptable carrier.

In another preferred embodiment, the pharmaceutical composition further comprises one or more therapeutic agents selected from the group consisting of: PD-1 inhibitors (e.g., nivolumab, pembrolizumab, pidilizumab, cemipimab, JS-001, SHR-120, BGB-A317, IBI-308, GLS-010, GB-226, STW204, HX008, HLX10, BAT 1306, AK105, LZM 009, or biologically similar drugs thereof), PD-L1 inhibitors (e.g., durvalumab, atezolizumab, avelumab, CS1001, KN035, HLX20, SHR-1316, BGB-A333, JS003, CS1003, A167, F520, GR1405, MSB2311, or biologically similar drugs thereof), CD20 antibodies (e.g., rituximab, OBINUzumab, OFTUUMAB, TUMAb, tosumumab, TOSITUMOMAb, 131I-TUMOMIB, Humbib 483 90, ALOMIJUSHIMAb 29-90, ALMIIJK-29, ALMIIJX 008, ESK-35, ESK-L-35, ESK-S-D21, ESK-S-D2, ESK-S-D-S-D-S-K-S-K-S-K-S-K-S-, Alectonib, Brigatinib, Lorlatinib, oxcarinib), PI3K inhibitors (e.g., Idelalisib, Duvelisib, Dactolisib, Taselisib, Bimiralisib, Omipaliib, Buparlisib, etc.), BTK inhibitors (e.g., Ibrutinib, Tirabutinib, Acalabastib, Zanbutrutinib, Vecabutinib, etc.), EGFR inhibitors (e.g., Afatinib, Gefitinib, Erlottinib, Lapatinib, Dacomitib, Icotininib, Canertinib, Sapinotinib, Naquratinib, Pyrotinib, Rocilitiniib, Osimicinib, etc.), inhibitors (e.g., Sorafenib, Parraertinib, Leutinib 6265, Lipocinib, etc.), inhibitors (e.g., Lipocinib, VEGF, Lipocinib, etc.), inhibitors (e, Lipocinib, etc.), inhibitors, Lipocinib, VEGF, etc. (e, Lipocinib, etc.), inhibitors, Lipocinib, e, etc. (e, etc.), inhibitors, e, Lipocinib, e, etc. (e, e.g., Nonocitinib, Lipocinib, e, e.g., Nonocitinib, VEGF-10-III inhibitors, VEGF-III inhibitors, e, e.g., Nonocitinib, e, e.g., Lipocinib, e.g., Nonocitinib, e, e.g., Lipocinib, VEGF-III inhibitors, e.g., Lipocinib, e, VEGF-III inhibitors (e.g., Lipocinib, VEGF-III inhibitors, e, e.g., Lipocinib, VEGF-III inhibitors, e.g., Lipocinib, e, e.g., Lipocinib, VEGF-III inhibitors, e, e.g., Lipocinib, VEGF-III inhibitors (e.g., Lipocinib, VEGF-III inhibitors, VEGF-10-III inhibitors, VEGF-III inhibitors, e, e.g., Lipocinib, III inhibitors (e, III inhibitors, III-III inhibitors, III.

In a fourth aspect of the present invention, there is provided a use of the compound according to the first aspect, or a stereoisomer, a tautomer, a crystal form, a pharmaceutically acceptable salt, a hydrate, a solvate, or a prodrug thereof, or the pharmaceutical composition according to the third aspect, for the preparation of a pharmaceutical composition for the prevention and/or treatment of a disease associated with the activity or expression amount of SOS 1.

In another preferred embodiment, the disease is cancer.

In another preferred embodiment, the cancer is selected from: lung cancer, breast cancer, prostate cancer, esophageal cancer, colorectal cancer, bone cancer, kidney cancer, stomach cancer, liver cancer, colorectal cancer, melanoma, lymphoma, leukemia, brain tumor, myeloma, soft tissue sarcoma, pancreatic cancer, and skin cancer.

In a fifth aspect of the invention, there is provided a method of non-diagnostic, non-therapeutic inhibition of SOS1, comprising the steps of: administering to a patient in need thereof an effective amount of a compound, stereoisomer, tautomer, crystalline form, pharmaceutically acceptable salt, hydrate, solvate, or prodrug thereof, as described above, or administering a pharmaceutical composition as described above.

It is to be understood that within the scope of the present invention, the above-described features of the present invention and those specifically described below (e.g., in the examples) may be combined with each other to form new or preferred embodiments. Not to be reiterated herein, but to the extent of space.

Drawings

Is free of

Detailed Description

The present inventors have conducted extensive and intensive studies for a long time and have unexpectedly found a novel class of compounds which selectively inhibit SOS1 and/or exhibit improved pharmacodynamic properties. On this basis, the inventors have completed the present invention.

Term(s) for

In the present invention, unless otherwise specified, the terms used have the ordinary meaning known to those skilled in the art.

The term "alkyl" refers to straight or branched chain or cyclic alkyl groups containing 1 to 20 carbon atoms, such as 1 to 18 carbon atoms, especially 1 to 18 carbon atoms. Typical "alkyl" groups include methyl, ethyl, propyl, isopropyl, n-butyl, t-butyl, isobutyl, n-butyl,

Pentyl, isopentyl, heptyl, 4-dimethylpentyl, octyl, 2, 4-trimethylpentyl, nonyl, decyl, undecyl, dodecyl and the like.

The term "C1-C18 alkyl" refers to straight or branched chain or cyclic alkyl groups including from 1 to 18 carbon atoms, such as methyl, ethyl, propyl, isopropyl

N-butyl, t-butyl, isobutyl (e.g. butyl, isobutyl)

) N-pentyl, isopentyl, n-hexyl, isohexyl, n-heptyl, isoheptyl. "substituted alkyl" means an alkyl group which is substituted at one or more positions, especially 1 to 4 substituents, and may be substituted at any position. Typical substitutions include, but are not limited to, one or more of the following groups: such as hydrogen, deuterium, halogen (e.g. monohalogen substituents or polyhalo substituents, the latter being trifluoromethyl or containing Cl ₃ Alkyl group of (a), nitrile group, nitro group, oxygen (e.g., ═ O), trifluoromethyl group, trifluoromethoxy group, cycloalkyl group, alkenyl group, cycloalkenyl group, alkynyl group, heterocycle, aromatic ring, OR _a 、SR _a 、S(＝O)R _e 、S(＝O) ₂ R _e 、P(＝O) ₂ R _e 、S(＝O) ₂ OR _e ,P(＝O) ₂ OR _e 、NR _b R _c 、NR _b S(＝O) ₂ R _e 、NR _b P(＝O) ₂ R _e 、S(＝O) ₂ NR _b R _c 、P(＝O) ₂ NR _b R _c 、C(＝O)OR _d 、C(＝O)R _a 、C(＝O)NR _b R _c 、OC(＝O)R _a 、OC(＝O)NR _b R _c 、NR _b C(＝O)OR _e ,NR _d C(＝O)NR _b R _c 、NR _d S(＝O) ₂ NR _b R _c 、NR _d P(＝O) ₂ NR _b R _c 、NR _b C(＝O)R _a Or NR _b P(＝O) ₂ R _e Wherein R is present therein _a May independently represent hydrogen, deuterium, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle or aromatic ring, R _b 、R _c And R _d May independently represent hydrogen, deuterium, alkyl, cycloalkyl, heterocycle or aromatic ring, or R _b And R _c Together with the N atom may form a heterocyclic ring; r _e May independently represent hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle or aromatic ring. The above-mentioned typical substituents such as alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle or aromatic ring may be optionally substituted.

The term "alkylene" refers to a group formed by removing a hydrogen atom from an "alkyl" group, such as methylene, ethylene, propylene, isopropylene (e.g.

) Butylene (e.g. butyl oxide)

) Pentylene (e.g. ethylene)

) Hexamethylene (e.g. hexamethylene)

) Heptylene (e.g. ethylene)

) And the like.

The term "cycloalkyl" refers to a fully saturated cyclic hydrocarbon group comprising 1 to 4 rings containing 3 to 8 carbon atoms in each ring. "substituted cycloalkyl" means that one or more positions in the cycloalkyl group are substituted, especially 1 to 4 substituents, which may be substituted at any position. Typical substitutions include, but are not limited to, one or more of the following groups: such as hydrogen, deuterium, halogen (e.g., monohalogen substituents or polyhalo substituents, the latter such as trifluoromethyl or containing Cl ₃ Alkyl group of (a), nitrile group, nitro group, oxygen (e.g., ═ O), trifluoromethyl group, trifluoromethoxy group, cycloalkyl group, alkenyl group, cycloalkenyl group, alkynyl group, heterocycle, aromatic ring, OR _a 、SR _a 、S(＝O)R _e 、S(＝O) ₂ R _e 、P(＝O) ₂ R _e 、S(＝O) ₂ OR _e ,P(＝O) ₂ OR _e 、NR _b R _c 、NR _b S(＝O) ₂ R _e 、NR _b P(＝O) ₂ R _e 、S(＝O) ₂ NR _b R _c 、P(＝O) ₂ NR _b R _c 、C(＝O)OR _d 、C(＝O)R _a 、C(＝O)NR _b R _c 、OC(＝O)R _a 、OC(＝O)NR _b R _c 、NR _b C(＝O)OR _e ,NR _d C(＝O)NR _b R _c 、NR _d S(＝O) ₂ NR _b R _c 、NR _d P(＝O) ₂ NR _b R _c 、NR _b C(＝O)R _a Or NR _b P(＝O) ₂ R _e Wherein R is present therein _a May independently represent hydrogen, deuterium, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle or aromatic ring, R _b 、R _c And R _d May independently represent hydrogen, deuterium, alkyl, cycloalkyl, heterocycle or aromatic ring, or R _b And R _c Together with the N atom may form a heterocyclic ring;R _e may independently represent hydrogen, deuterium, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle or aromatic ring. The above typical substituents may be optionally substituted. Typical substitutions also include spirocyclic, bridged or fused ring substituents, especially spirocycloalkyl, spirocycloalkenyl, spiroheterocyclic (excluding heteroaromatic rings), bridged cycloalkyl, bridged alkenyl, bridged heterocyclic (excluding heteroaromatic rings), fused cycloalkyl, fused alkenyl, fused heterocyclyl or fused aromatic ring groups, which may be optionally substituted. Any two or more atoms on the ring may be further joined to other cycloalkyl, heterocyclyl, aryl, and heteroaryl groups.

The term "cycloalkylene" refers to a group formed by a cycloalkyl group minus two hydrogen atoms, such as:

the term "alkylenecycloalkylene" refers to a group formed by the above-mentioned cycloalkylalkyl or alkylcycloalkyl, minus two hydrogen atoms, wherein "C1-C18 alkylene C3-C20 cycloalkylene" or "C3-C20 cycloalkylene C1-C18 alkylene" has the same meaning, preferably C1-C6 alkylene C3-C12 cycloalkylene, including but not limited to:

and the like.

The term "heterocyclyl" refers to a fully saturated or partially unsaturated cyclic group (including but not limited to, e.g., a 3-7 membered monocyclic, 6-11 membered bicyclic, or 8-16 membered tricyclic ring system) in which at least one heteroatom is present in the ring having at least one carbon atom. Each heteroatom-containing heterocyclic ring may carry 1,2, 3 or 4 heteroatoms selected from nitrogen atoms, oxygen atoms or sulfur atoms, wherein the nitrogen or sulfur atoms may be oxidized and the nitrogen atoms may also be quaternized. The heterocyclic group may be attached to any heteroatom or residue of a carbon atom of the ring or ring system molecule. Typical monocyclic heterocycles include, but are not limited to, azetidinyl, pyrrolidinyl, oxetanyl, pyrazolinyl, imidazolinyl, imidazolidinyl, oxazolidinyl, isoxazolidinyl, thiazolidinyl, isothiazolidinyl, tetrahydrofuranyl, piperidinyl, piperazinyl, 2-oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolidinyl, hexahydroazepinyl, 4-piperidyl, tetrahydropyranyl, morphinyl, thiomorpholinyl sulfoxide, thiomorpholinyl sulfone, 1, 3-dioxanyl, and tetrahydro-1, 1-dioxythiophene, and the like. Polycyclic heterocyclic groups include spiro, fused and bridged heterocyclic groups; wherein the heterocyclic groups of the spiro, fused and bridged rings are optionally linked to other groups by single bonds, or are further linked to other cycloalkyl, heterocyclic, aryl and heteroaryl groups by any two or more atoms in the ring; the heterocyclic group may be substituted or unsubstituted, and when substituted, the substituent is preferably one or more groups independently selected from alkyl, deuterated alkyl, haloalkyl, alkoxy, haloalkoxy, alkenyl, alkynyl, alkylthio, alkylamino, halogen, amino, nitro, hydroxy, mercapto, cyano, cycloalkyl, heterocyclyl, aryl, heteroaryl, cycloalkylthio, oxo, carboxy, and carboxylate groups, wherein any two or more atoms on the ring may be further ring-connected to other cycloalkyl, heterocyclyl, aryl, and heteroaryl groups, such as 5-12 membered heterocyclylphenyl, 5-6 membered heteroaryl, 5-15 membered heterocyclylphenyl, and 5-6 membered heteroaryl.

The term "heterocyclylene" refers to a group formed by removing two hydrogen atoms from the above-mentioned heterocyclic group, and includes, but is not limited to:

and the like.

The term "heterocycloalkylene alkylene" refers to a cycloalkylalkyl or alkylcycloalkyl group formed by removal of two hydrogen atoms, wherein "4-20 membered heterocycloalkylene C1-C18 alkylene" or "C1-C18 alkylene 4-20 membered heterocycloalkylene" has the same meaning, preferably 4-12 membered heterocycloalkylene C1-6 alkylene, including but not limited to:

and the like.

The term "aryl" refers to aromatic cyclic hydrocarbon groups having 1 to 5 rings, especially monocyclic and bicyclic groups such as phenyl, biphenyl or naphthyl. Where the aromatic ring contains two or more aromatic rings (bicyclic, etc.), the aromatic rings of the aryl group may be linked by a single bond (e.g., biphenyl), or fused (e.g., naphthalene, anthracene, etc.). "substituted aryl" means that one or more positions in the aryl group are substituted, especially 1 to 3 substituents, which may be substituted at any position. Typical substitutions include, but are not limited to, one or more of the following groups: such as hydrogen, deuterium, halogen (e.g. monohalogen substituents or polyhalo substituents, the latter being trifluoromethyl or containing Cl ₃ Alkyl group of (a), nitrile group, nitro group, oxygen (e.g., ═ O), trifluoromethyl group, trifluoromethoxy group, cycloalkyl group, alkenyl group, cycloalkenyl group, alkynyl group, heterocycle, aromatic ring, OR _a 、SR _a 、S(＝O)R _e 、S(＝O) ₂ R _e 、P(＝O) ₂ R _e 、S(＝O) ₂ OR _e ,P(＝O) ₂ OR _e 、NR _b R _c 、NR _b S(＝O) ₂ R _e 、NR _b P(＝O) ₂ R _e 、S(＝O) ₂ NR _b R _c 、P(＝O) ₂ NR _b R _c 、C(＝O)OR _d 、C(＝O)R _a 、C(＝O)NR _b R _c 、OC(＝O)R _a 、OC(＝O)NR _b R _c 、NR _b C(＝O)OR _e ,NR _d C(＝O)NR _b R _c 、NR _d S(＝O) ₂ NR _b R _c 、NR _d P(＝O) ₂ NR _b R _c 、NR _b C(＝O)R _a Or NR _b P(＝O) ₂ R _e Wherein R is present therein _a May independently represent hydrogen, deuterium, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle or aromatic ring, R _b 、R _c And R _d May independently represent hydrogen, deuterium, alkyl, cycloalkyl, heterocycle or aromatic ring, or R _b And R _c Together with the N atom may form a heterocyclic ring; r _e May independently represent hydrogen, deuterium, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, heterocycle or aromatic ring. The above typical substituents may be optionally substituted. Typical substitutions also include fused ring substituents, especially fused ring alkyl, fused ring alkenyl, fused ring heterocyclyl or fused ring aromatic ring groups, which cycloalkyl, cycloalkenyl, heterocyclyl and heterocyclylaryl groups may be optionally substituted.

The term "heteroaryl" refers to a heteroaromatic system comprising 1-4 heteroatoms, 5-14 ring atoms, wherein the heteroatoms are selected from oxygen, nitrogen and sulfur. The heteroaryl group is preferably a 5-to 10-membered ring, more preferably a 5-or 6-membered ring, for example, pyrrolyl, pyrazolyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, thiadiazolyl, isothiazolyl, furyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, triazolyl, tetrazolyl, and the like. "heteroaryl" may be substituted or unsubstituted, and when substituted, the substituents are preferably one or more groups independently selected from alkyl, deuterated alkyl, haloalkyl, alkoxy, haloalkoxy, alkenyl, alkynyl, alkylthio, alkylamino, halogen, amino, nitro, hydroxy, mercapto, cyano, cycloalkyl, heterocyclyl, aryl, heteroaryl, cycloalkylthio, oxo, carboxyl, and carboxylate.

The term "C1-C18 alkoxy" refers to a straight or branched chain or cyclic alkoxy group having 1 to 18 carbon atoms, including without limitation methoxy, ethoxy, propoxy, isopropoxy, butoxy, and the like. Preferably C1-C8 alkoxy, more preferably C1-C6 alkoxy.

The term "C1-C18 alkyleneoxy" refers to the group resulting from the removal of one hydrogen atom from the "C1-C18 alkoxy group".

The term "halogen" or "halo" refers to chlorine, bromine, fluorine, iodine.

The term "halo" means substituted with halogen.

The term "deuterated" refers to a substitution by deuterium.

The term "hydroxy" refers to a group with the structure OH.

The term "nitro" refers to a group with the structure NO ₂ A group of (1).

The term "cyano" refers to a group with the structure CN.

The term "ester group" refers to a group with the structure-COOR, wherein R represents hydrogen, alkyl or substituted alkyl, cycloalkyl or substituted cycloalkyl, cycloalkenyl or substituted cycloalkenyl, aryl or substituted aryl, heterocyclic or substituted heterocyclic.

The term "amine" refers to a group having the structure-NRR ', where R and R' may independently represent hydrogen, alkyl or substituted alkyl, cycloalkyl or substituted cycloalkyl, cycloalkenyl or substituted cycloalkenyl, aryl or substituted aryl, heterocyclic or substituted heterocyclic, as defined above. R and R' may be the same or different in the dialkylamine fragment.

The term "amido" refers to a group with the structure-CONRR ', where R and R' may independently represent hydrogen, alkyl or substituted alkyl, cycloalkyl or substituted cycloalkyl, cycloalkenyl or substituted cycloalkenyl, aryl or substituted aryl, heterocycle or substituted heterocycle as defined above. R and R' may be the same or different in the dialkylamine fragment.

The term "sulfonamide" refers to a sulfonamide group having the structure-SO ₂ NRR 'wherein R and R' may independently represent hydrogen, alkylA group or substituted alkyl, cycloalkyl or substituted cycloalkyl, cycloalkenyl or substituted cycloalkenyl, aryl or substituted aryl, heterocycle or substituted heterocycle as defined above. R and R' may be the same or different in the dialkylamine fragment.

The term "ureido" refers to a group having the structure — NRCONR 'R ", where R, R' and R" may independently represent hydrogen, alkyl or substituted alkyl, cycloalkyl or substituted cycloalkyl, cycloalkenyl or substituted cycloalkenyl, aryl or substituted aryl, heterocyclic or substituted heterocyclic, as defined above. R, R' and R "may be the same or different in the dialkylamine fragment.

The term "alkylaminoalkyl" refers to a group having the structure-RNHR ', wherein R and R' may independently represent hydrogen, alkyl or substituted alkyl, cycloalkyl or substituted cycloalkyl, cycloalkenyl or substituted cycloalkenyl, aryl or substituted aryl, heterocycle or substituted heterocycle as defined above. R and R' may be the same or different.

The term "dialkylaminoalkyl" refers to a group having the structure-RNHR 'R ", where R, R' and R" can independently represent alkyl or substituted alkyl, cycloalkyl or substituted cycloalkyl, cycloalkenyl or substituted cycloalkenyl, aryl or substituted aryl, a heterocycle or a substituted heterocycle, as defined above. R, R' and R "may be the same or different in the dialkylamine fragment.

The term "heterocyclylalkyl" refers to a group having the structure-RR', wherein R may independently represent alkyl or substituted alkyl, cycloalkyl or substituted cycloalkyl, cycloalkenyl or substituted cycloalkenyl, aryl or substituted aryl; r' represents a heterocycle or a substituted heterocycle.

In the present invention, the term "substituted" means that one or more hydrogen atoms on a specified group are replaced with a specified substituent. Particular substituents are those described correspondingly in the preceding text or as present in the examples. Unless otherwise specified, a certain substituted group may have one substituent selected from a specific group at any substitutable site of the group, and the substituents may be the same or different at each position. It will be understood by those skilled in the art that the combinations of substituents contemplated by the present invention are those that are stable or chemically achievable. Such substituents are for example (but not limited to): halogen, hydroxyl, cyano, carboxyl (-COOH), C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C3-C8 cycloalkyl, 3-to 12-membered heterocyclic, aryl, heteroaryl, C1-C8 aldehyde, C2-C10 acyl, C2-C10 ester, amine, C1-C6 alkoxy, C1-C10 sulfonyl, and C1-C6 ureido, and the like.

Unless otherwise stated, it is assumed that any heteroatom that is not in a valence state has sufficient hydrogen to replenish its valence state.

When the substituent is a non-terminal substituent, it is a subunit of the corresponding group, for example, alkyl corresponds to alkylene, cycloalkyl corresponds to cycloalkylene, heterocyclyl corresponds to heterocyclylene, alkoxy corresponds to alkyleneoxy, and the like.

Active ingredient

As used herein, "compounds of the invention" refers to compounds of formula I, and also includes stereoisomers or optical isomers, pharmaceutically acceptable salts, prodrugs or solvates of the compounds of formula I.

In the present invention, the compounds of formula (I) have the following structure:

wherein, X, Y, U, V, ring A, ring B, R ¹ 、R ² 、R ³ 、R ⁴ 、R ⁵ M and n are as defined above.

Preferably, in the formula I,

moieties are selected from:

preferably, the compound of formula I has the structure of formula (II):

Preferably, the compound of formula I has the structure of formula (III):

Preferably, the compound of formula I has the structure shown in formula (IV):

Preferably, the compound of formula I has the structure of formula (V):

in the formula:

wherein the above substitution means substitution with one or more groups selected from the group consisting of: hydrogen, deuterium, C ₁ -C ₁₈ Alkyl, deuterated C ₁ -C ₁₈ Alkyl, halo C ₁ -C ₁₈ Alkyl, halo C ₁ -C ₁₈ Alkyl hydroxy, C ₃ -C ₂₀ Cycloalkyl radical, C ₁ -C ₁₈ Alkoxy, deuterated C ₁ -C ₁₈ Alkoxy, halo C ₁ -C ₁₈ Alkoxy radical, C ₆ -C ₁₄ Aryl, 5-14 membered heteroaryl, 4-20 membered heterocyclyl, halogen, nitro, hydroxy, cyano, ester, amine, amide, sulfonamide, or ureido;

R ¹ 、R ² 、R ³ ring B, m and n are as defined above.

Preferably, the compound of formula I has the structure of formula (VI):

in the formula (I), the compound is shown in the specification,

R ¹ 、R ² 、R ³ ring B, m, n and R ^y Is as defined above.

Preferably, in the formulae I-VI, R ¹ 、R ² Each independently selected from the group consisting of: hydrogen, deuterium, halogen, cyano, oxo, substituted or unsubstituted C ₂ -C ₄ Alkenyl, substituted or unsubstituted C ₂ -C ₄ Alkynyl, substituted or unsubstituted C ₁ -C ₆ Alkyl, substituted or unsubstituted C ₃ -C ₆ Cycloalkyl, substituted or unsubstituted 4-6 membered heterocycloalkyl, substituted or unsubstituted C ₁ -C ₆ Alkylene radical C ₃ -C ₁₃ Cycloalkylene, substituted or unsubstituted C ₁ -C ₆ Alkylene 4-12 membered heterocycloalkylene, - (CH) ₂ ) _h O(CH ₂ ) _p R ⁶ 、-(CH ₂ ) _h SR ⁶ 、-(CH ₂ ) _h COR ⁶ 、-(CH ₂ ) _h C(O)OR ⁶ 、-(CH ₂ ) _h S(O) _q R ⁶ 、-(CH ₂ ) _h NR ⁶ R ⁷ 、-(CH ₂ ) _h C(O)NR ⁶ R ⁷ 、-(CH ₂ ) _h NR ⁶ C(O)R ⁷ 、-(CH ₂ ) _h NR ⁶ C(O)NR ⁷ R ⁸ 、-(CH ₂ ) _h S(O) _q NR ⁶ R ⁷ 、-(CH ₂ ) _h NR ⁶ S(O) _q R ⁷ 、-(CH ₂ ) _h NR ⁶ S(O) _q NR ⁷ R ⁸ ；R ⁶ 、R ⁷ And R ⁸ Each independently selected from the group consisting of: hydrogen, substituted or unsubstituted C ₁ -C ₆ Alkyl, substituted or unsubstituted C ₃ -C ₆ Cycloalkyl, substituted or unsubstituted 4-6 membered heterocyclyl, substituted or unsubstituted C ₆ -C ₁₄ Aryl and substituted or unsubstituted 5-14 membered heteroaryl; or in- (CH) ₂ ) _h NR ⁶ R ⁷ 、-(CH ₂ ) _h C(O)NR ⁶ R ⁷ 、-(CH ₂ ) _h S(O) _q NR ⁶ R ⁷ In, R ⁶ And R ⁷ And the adjacent N atom thereof form a substituted or unsubstituted 4-8 membered heterocyclic group; or in- (CH) ₂ ) _h NR ⁶ C(O)R ⁷ 、-(CH ₂ ) _h NR ⁶ C(O)NR ⁷ R ⁸ 、-(CH ₂ ) _h NR ⁶ S(O) _q R ⁷ 、-(CH ₂ ) _h NR ⁶ S(O) _q NR ⁷ R ⁸ In, R ⁷ And R ⁸ Is cyclized with its adjacent N atom to form a substituted or unsubstituted 4-8 membered heterocyclic group, or R ⁶ And R ⁷ Ring-closing with its adjacent atom to form a substituted or unsubstituted 4-8 membered heterocyclic group;

h is 0,1, 2,3, 4,5 or 6;

R ³ selected from the group consisting of substituted or unsubstituted: phenyl and 5-6 membered heteroaryl, wherein R ³ Wherein said substitution is by one or more groups selected from the group consisting of: c ₁ -C ₆ Alkyl, deuterated C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl hydroxy, C ₃ -C ₆ Cycloalkyl radical, C ₁ -C ₆ Alkoxy, deuterated C ₁ -C ₆ Alkoxy, halo C ₁ -C ₆ Alkoxy, halogen, nitro, hydroxy, oxo, cyano, ester, amine, amide, sulfonamide, or urea;

ring B is selected from the group consisting of substituted or unsubstituted: c ₃ -C ₁₀ Cycloalkyl, 4-15 membered heterocyclyl, phenyl and 5-6 membered heteroaryl, wherein said substitution in ring B means substitution by one or more groups selected from the group consisting of: c ₁ -C ₆ Alkyl, deuterated C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl hydroxy, C ₃ -C ₆ Cycloalkyl radical, C ₁ -C ₆ Alkoxy, deuterated C ₁ -C ₆ Alkoxy, halo C ₁ -C ₆ Alkoxy, halogen, nitro, hydroxyl, oxo, cyano, ester, amine, amide, sulfonamide, or urea.

The salts which the compounds of the invention may form are also within the scope of the invention. Unless otherwise indicated, the compounds of the present invention are understood to include salts thereof. The term "salt" as used herein refers to a salt formed from an inorganic or organic acid and a base in either an acidic or basic form. Furthermore, when a compound of the present invention contains a basic moiety, including but not limited to pyridine or imidazole, and an acidic moiety, including but not limited to carboxylic acid, zwitterions ("inner salts") that may form are included within the scope of the term "salt(s)". Pharmaceutically acceptable (i.e., non-toxic, physiologically acceptable) salts are preferred, although other salts are also useful, e.g., in isolation or purification steps during manufacture. The compounds of the invention may form salts, for example, by reacting compound I with an amount of acid or base, e.g. an equivalent amount, and salting out in a medium, or lyophilizing in an aqueous solution.

The compounds of the invention may contain basic moieties, including but not limited to amine or pyridine or imidazole rings, which may form salts with organic or inorganic acids. Typical acids which may form salts include acetates (e.g. with acetic acid or trihaloacetic acid such as trifluoroacetic acid), adipates, alginates, ascorbates, aspartates, benzoates, benzenesulfonates, bisulfates, borates, butyrates, citrates, camphorates, camphorsulfonates, cyclopentanepropionates, diglycolates, dodecylsulfates, ethanesulfonates, fumarates, glucoheptonates, glycerophosphates, hemisulfates, heptanoates, hexanoates, hydrochlorides, hydrobromides, hydroiodides, hydroxyethanesulfonates (e.g. 2-hydroxyethanesulfonates), lactates, maleates, methanesulfonates, naphthalenesulfonates (e.g. 2-naphthalenesulfonates), nicotinates, nitrates, oxalates, pectinates, persulfates, phenylpropionates (e.g. 3-phenylpropionates), phosphates, propionates, citrates, and the like, Picrates, pivalates, propionates, salicylates, succinates, sulfates (e.g., with sulfuric acid), sulfonates, tartrates, thiocyanates, tosylates, e.g., p-toluenesulfonate, dodecanoate, and the like

Acidic moieties that certain compounds of the present invention may contain, including but not limited to carboxylic acids, may form salts with various organic or inorganic bases. Typical salts with bases include ammonium salts, alkali metal salts such as sodium, lithium, potassium salts, alkaline earth metal salts such as calcium, magnesium salts, and salts with organic bases (e.g., organic amines) such as benzathine, dicyclohexylamine, hydrabamine (salt with N, N-bis (dehydroabietyl) ethylenediamine), N-methyl-D-glucamine, N-methyl-D-glucamide, t-butylamine, and salts with amino acids such as arginine, lysine, and the like. The basic nitrogen-containing groups may be combined with halide quaternary ammonium salts, such as small molecule alkyl halides (e.g., methyl, ethyl, propyl, and butyl chlorides, bromides, and iodides), dialkyl sulfates (e.g., dimethyl, diethyl, dibutyl, and diamyl sulfates), long chain halides (e.g., decyl, dodecyl, tetradecyl, and tetradecyl chlorides, bromides, and iodides), aralkyl halides (e.g., benzyl and phenyl bromides), and the like.

Prodrugs and solvates of the compounds of the invention are also contemplated. The term "prodrug" as used herein refers to a compound that undergoes chemical conversion by metabolic or chemical processes to yield a compound, salt, or solvate of the present invention when used in the treatment of a related disease. The compounds of the present invention include solvates, such as hydrates.

The compounds, salts or solvates of the invention may exist in tautomeric forms (e.g. amides and imino ethers). All of these tautomers are part of the present invention.

All stereoisomers of the compounds (e.g., those asymmetric carbon atoms that may exist due to various substitutions), including enantiomeric and diastereomeric forms thereof, are contemplated within the invention. The individual stereoisomers of the compounds of the invention may not be present in combination with the other isomers (e.g. as a pure or substantially pure optical isomer having a particular activity), or may be present as a mixture, e.g. as a racemate, or as a mixture with all or a portion of the other stereoisomers. The chiral center of the invention has two S or R configurations, and is defined by the International Union of theory and applied chemistry (IUPAC) proposed in 1974. The racemic forms can be resolved by physical methods such as fractional crystallization, or by separation of the crystals by derivatization into diastereomers, or by chiral column chromatography. The individual optical isomers can be obtained from the racemates by any suitable method, including, but not limited to, conventional methods such as salt formation with an optically active acid followed by crystallization.

The compounds of the present invention, obtained by preparing, isolating and purifying the compound in sequence, have a weight content of 90% or more, for example, 95% or more, 99% or more ("very pure" compounds), as set forth in the text. Such "very pure" compounds of the invention are also part of the invention herein.

All configurational isomers of the compounds of the invention are within the scope of the invention, whether in mixture, pure or very pure form. The definition of compounds in the present invention encompasses both cis (Z) and trans (E) olefin isomers, as well as cis and trans isomers of carbocyclic and heterocyclic rings.

Throughout the specification, groups and substituents may be selected to provide stable fragments and compounds.

Specific functional groups and definitions of chemical terms are detailed below. For purposes of the present invention, the chemical Elements are compatible with the Periodic Table of the Elements, CAS version, Handbook of Chemistry and Physics,75 ^th D. as defined in. The definition of a particular functional group is also described herein. In addition, the basic principles of Organic Chemistry, as well as specific functional groups and reactivities are also described in "Organic Chemistry", Thomas Sorrell, University Science Books, Sausaltito: 1999, which is incorporated by reference in its entirety.

Certain compounds of the present invention may exist in specific geometric or stereoisomeric forms. The present invention encompasses all compounds, including cis and trans isomers, R and S enantiomers, diastereomers, (D) isomer, (L) isomer, racemic mixtures and other mixtures thereof. Further the asymmetric carbon atom may represent a substituent such as an alkyl group. All isomers, as well as mixtures thereof, are encompassed by the present invention.

According to the present invention, the mixture of isomers may contain a variety of isomer ratios. For example, in a mixture of only two isomers, the following combinations are possible: 50:50, 60:40, 70:30, 80:20, 90:10, 95:5, 96:4, 97:3, 98:2, 99:1, or 100:0, all ratios of isomers are within the scope of the invention. Similar ratios, as well as ratios that are mixtures of more complex isomers, are also within the scope of the invention, as would be readily understood by one of ordinary skill in the art.

The invention also includes isotopically-labelled compounds, equivalent to the original compoundsDisclosed herein. In practice, however, it will often occur that one or more atoms are replaced by an atom having a different atomic weight or mass number. Examples of isotopes that can be listed as compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, sulfur, fluorine and chlorine, respectively ² H、 ³ H、 ¹³ C、 ¹¹ C、 ¹⁴ C、 ¹⁵ N、 ¹⁸ O、 ¹⁷ O、 ³¹ P、 ³² P、 ³⁵ S、 ¹⁸ F and ³⁶ and (4) Cl. The compounds of the present invention, or enantiomers, diastereomers, isomers, or pharmaceutically acceptable salts or solvates thereof, wherein isotopes or other isotopic atoms containing such compounds are within the scope of the present invention. Certain isotopically-labelled compounds of the invention, e.g. ³ H and ¹⁴ among these, the radioactive isotope of C is useful in tissue distribution experiments of drugs and substrates. Tritium, i.e. ³ H and carbon-14, i.e. ¹⁴ C, their preparation and detection are relatively easy. Is the first choice among isotopes. Furthermore, heavier isotopes such as deuterium are substituted, i.e. ² H, due to its good metabolic stability, may be advantageous in certain therapies, such as increased half-life in vivo or reduced dose, and therefore, may be preferred in certain circumstances. Isotopically labeled compounds can be prepared by conventional methods by substituting a readily available isotopically labeled reagent for a non-isotopically labeled reagent using the protocols disclosed in the examples.

If it is desired to design the synthesis of a particular enantiomer of a compound of the invention, it may be prepared by asymmetric synthesis or by derivatization with chiral auxiliary agents, separation of the resulting diastereomeric mixture and removal of the chiral auxiliary agent to give the pure enantiomer. Alternatively, if the molecule contains a basic functional group, such as an amino acid, or an acidic functional group, such as a carboxyl group, diastereomeric salts can be formed therewith with an appropriate optically active acid or base, and the isolated enantiomers can be obtained in pure form by conventional means such as fractional crystallization or chromatography.

As described herein, the compounds of the present invention can be substituted with any number of substituents or functional groups to extend their inclusion range. In general, the term "substituted", whether occurring before or after the term "optional", in the formula of the present invention including substituents, means that the hydrogen radical is replaced with a substituent of the indicated structure. When a plurality of the specified structures are substituted at a position with a plurality of the specified substituents, each position of the substituents may be the same or different. The term "substituted" as used herein includes all permissible substitutions of organic compounds. In a broad sense, permissible substituents include acyclic, cyclic, branched unbranched, carbocyclic and heterocyclic, aromatic and nonaromatic organic compounds. In the present invention, the heteroatom nitrogen may have a hydrogen substituent or any permissible organic compound described hereinabove to supplement its valence state. Furthermore, the present invention is not intended to be limited in any way as to the permissible substitution of organic compounds. The present invention recognizes that the combination of substituents and variable groups is excellent in the treatment of diseases, such as infectious diseases or proliferative diseases, in the form of stable compounds. The term "stable" as used herein refers to compounds that are stable enough to maintain the structural integrity of the compound when tested for a sufficient period of time, and preferably are effective for a sufficient period of time, and are used herein for the purposes described above.

Metabolites of the compounds referred to herein and pharmaceutically acceptable salts thereof, as well as prodrugs that are convertible in vivo into the structures of the compounds referred to herein and pharmaceutically acceptable salts thereof, are also encompassed by the claims herein.

Preparation method

The following describes more specifically the processes for the preparation of the compounds of formula (I) according to the invention, but these particular processes do not constitute any limitation of the invention. The compounds of the present invention may also be conveniently prepared by optionally combining various synthetic methods described in the present specification or known in the art, and such combinations may be readily carried out by those skilled in the art to which the present invention pertains.

Typically, the process for the preparation of the compounds of the present invention is as follows, wherein the starting materials and reagents used are commercially available without specific reference.

Preferably, the compounds of the present invention are prepared by the following method

Method 1

(i) Reacting the compound of the formula (X-1) with acetic anhydride to obtain a compound of a formula (X-2);

method two

(iv) reacting the compound of formula (X-3) with a compound LG-X in the presence of a base (e.g., TEA, DIPEA, Py, DMAP, etc.) to give a compound of formula (X-5);

in the formula (I), the compound is shown in the specification,

LG is selected from: halogen, OTs, OMs or OTf;

LG-X is selected from: SOCl ₂ 、SO ₂ Cl ₂ 、POCl ₃ 、MsCl、TsCl、TfOH、TMSOTf、Tf ₂ O, or Ms ₂ O and the like

R ¹ 、R ² 、R ³ X, ring B, m and n are as defined above.

Pharmaceutical compositions and methods of administration

The pharmaceutical composition of the invention is used for preventing and/or treating the following diseases: inflammation, cancer, cardiovascular disease, infection, immunological disease, and metabolic disease.

The compounds of general formula (I) may be used in combination with other drugs known to treat or ameliorate similar conditions. When administered in combination, the mode of administration and dosage of the original drug may be maintained unchanged while the compound of formula I is administered simultaneously or subsequently. When the compound of formula I is administered simultaneously with one or more other drugs, it may be preferable to use a pharmaceutical composition containing both one or more known drugs and the compound of formula I. The pharmaceutical combination also includes administration of the compound of formula I in an overlapping time period with one or more other known drugs. When a compound of formula I is administered in a pharmaceutical combination with one or more other drugs, the dose of the compound of formula I or the known drug may be lower than the dose at which they are administered alone.

Drugs or active ingredients that may be used in combination with the compounds of formula (I) include, but are not limited to: PD-1 inhibitors (e.g., nivolumab, pembrolizumab, pidilizumab, cemiplimab, JS-001, SHR-120, BGB-A317, IBI-308, GLS-010, GB-226, STW204, HX008, HLX10, BAT 1306, AK105, LZM 009, or biologically similar drugs thereof), PD-L1 inhibitors (e.g., durvalumab, atezolizumab, avelumab, CS1001, KN035, HLX20, SHR-1316, BGB-A333, JS003, CS1003, A167, F520, GR1405, MSB2311, or biologically similar drugs thereof), CD20 antibodies (e.g., rituximab, obiumumab, ofutatumab, NI, tositumomab, 131-tositumomab, moylb, MOVITAb, MOXIB 483, TSM-90, TSI-52, TSI-102, TSI-35, TSI-102, TSI-35, TSI-102, TSI-II, TSI-10, TSI-II, TSI-II, TSI-II, TSI-II, TSI-II, TSI-II, TSI-II, TSI-II, TSI-II, TSI-II, TSI-II, TSI-II, TSI-II, TSI, alectonib, Brigatinib, Lorlatinib, oxcarinib), PI3K inhibitors (e.g., Idelalisib, Duvelisib, Dactolisib, Taselisib, Bimiralisib, Omipaliib, Buparlisib, etc.), BTK inhibitors (e.g., Ibrutinib, Tirabutinib, Acalabastib, Zanbutrutinib, Vecabutinib, etc.), EGFR inhibitors (e.g., Afatinib, Gefitinib, Erlottinib, Lapatinib, Dacomitib, Icotininib, Canertinib, Sapinotinib, Naquratinib, Pyrotinib, Rocilitiniib, Osimicinib, etc.), inhibitors (e.g., Sorafenib, Parraertinib, Leutinib 6265, Lipocinib, etc.), inhibitors (e.g., Lipocinib, VEGF, Lipocinib, etc.), inhibitors (e, Lipocinib, etc.), inhibitors, Lipocinib, VEGF, etc. (e, Lipocinib, etc.), inhibitors, Lipocinib, e, etc. (e, etc.), inhibitors, e, Lipocinib, e, etc. (e, e.g., Nonocitinib, Lipocinib, e, e.g., Nonocitinib, VEGF-10-III inhibitors, VEGF-III inhibitors, e, e.g., Nonocitinib, e, e.g., Lipocinib, e.g., Nonocitinib, e, e.g., Lipocinib, VEGF-III inhibitors, e.g., Lipocinib, e, VEGF-III inhibitors (e.g., Lipocinib, VEGF-III inhibitors, e, e.g., Lipocinib, VEGF-III inhibitors, e.g., Lipocinib, e, e.g., Lipocinib, VEGF-III inhibitors, e, e.g., Lipocinib, VEGF-III inhibitors (e.g., Lipocinib, VEGF-III inhibitors, VEGF-10-III inhibitors, VEGF-III inhibitors, e, e.g., Lipocinib, III inhibitors (e, III inhibitors, III-III inhibitors, III.

Dosage forms of the pharmaceutical compositions of the present invention include (but are not limited to): injection, tablet, capsule, aerosol, suppository, pellicle, dripping pill, topical liniment, controlled release type or delayed release type or nanometer preparation.

The pharmaceutical composition of the present invention comprises the compound of the present invention or a pharmacologically acceptable salt thereof in a safe and effective amount range and a pharmacologically acceptable excipient or carrier. Wherein "safe and effective amount" means: the amount of the compound is sufficient to significantly improve the condition without causing serious side effects. Typically, the pharmaceutical composition contains 1-2000mg of a compound of the invention per dose, more preferably, 10-1000mg of a compound of the invention per dose. Preferably, said "dose" is a capsule or tablet.

"pharmaceutically acceptable carrier" refers to: one or more compatible solid or liquid fillers or gel substances which are suitable for human use and must be of sufficient purity and sufficiently low toxicity. By "compatible" is meant herein that the components of the composition are capable of intermixing with and with the compounds of the present invention without significantly diminishing the efficacy of the compounds. Examples of pharmaceutically acceptable carrier moieties are cellulose and its derivatives (e.g., sodium carboxymethylcellulose, sodium ethylcellulose, cellulose acetate, etc.), gelatin, talc, solid lubricants (e.g., stearic acid, magnesium stearate), calcium sulfate, vegetable oils (e.g., soybean oil, sesame oil, peanut oil, olive oil, etc.), polyols (e.g., propylene glycol, glycerin, mannitol, sorbitol, etc.), emulsifiers (e.g., propylene glycol, glycerin, mannitol, sorbitol, etc.), and the like

) Wetting agents (e.g., sodium lauryl sulfate), coloring agents, flavoring agents, stabilizers, antioxidants, preservatives, pyrogen-free water, and the like.

The mode of administration of the compounds or pharmaceutical compositions of the present invention is not particularly limited, and representative modes of administration include (but are not limited to): oral, intratumoral, rectal, parenteral (intravenous, intramuscular or subcutaneous), and topical administration.

Solid dosage forms for oral administration include capsules, tablets, pills, powders and granules. In these solid dosage forms, the active compound is mixed with at least one conventional inert excipient (or carrier), such as sodium citrate or dicalcium phosphate, or with the following ingredients: (a) fillers or extenders, for example, starch, lactose, sucrose, glucose, mannitol and silicic acid; (b) binders, for example, hydroxymethyl cellulose, alginates, gelatin, polyvinylpyrrolidone, sucrose, and acacia; (c) humectants, for example, glycerol; (d) disintegrating agents, for example, agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain complex silicates, and sodium carbonate; (e) slow solvents, such as paraffin; (f) absorption accelerators, e.g., quaternary ammonium compounds; (g) wetting agents, such as cetyl alcohol and glycerol monostearate; (h) adsorbents, for example, kaolin; and (i) lubricants, for example, talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, or mixtures thereof. In capsules, tablets and pills, the dosage forms may also comprise buffering agents.

Solid dosage forms such as tablets, dragees, capsules, pills, and granules can be prepared using coatings and shells such as enteric coatings and other materials well known in the art. They may contain opacifying agents and the release of the active compound or compounds in such compositions may be delayed in release in a certain part of the digestive tract. Examples of embedding components which can be used are polymeric substances and wax-like substances. If desired, the active compound may also be in microencapsulated form with one or more of the above-mentioned excipients.

Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, solutions, suspensions, syrups or tinctures. In addition to the active compounds, the liquid dosage forms may contain inert diluents commonly employed in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, propylene glycol, 1, 3-butylene glycol, dimethylformamide and oils, in particular, cottonseed, groundnut, corn germ, olive, castor and sesame oils or mixtures of such materials and the like.

In addition to these inert diluents, the compositions can also contain adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.

Suspensions, in addition to the active compounds, may contain suspending agents as, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum methoxide and agar, or mixtures of these substances, and the like.

Compositions for parenteral injection may comprise physiologically acceptable sterile aqueous or anhydrous solutions, dispersions, suspensions or emulsions, and sterile powders for reconstitution into sterile injectable solutions or dispersions. Suitable aqueous and nonaqueous carriers, diluents, solvents or vehicles include water, ethanol, polyols and suitable mixtures thereof.

Dosage forms for topical administration of the compounds of the present invention include ointments, powders, patches, sprays, and inhalants. The active ingredient is mixed under sterile conditions with a physiologically acceptable carrier and any preservatives, buffers, or propellants which may be required if necessary.

The treatment methods of the present invention can be administered alone or in combination with other therapeutic means or agents.

When the pharmaceutical composition is used, a safe and effective amount of the compound of the present invention is suitable for mammals (such as human beings) to be treated, wherein the administration dose is a pharmaceutically-considered effective administration dose, and for a human body with a weight of 60kg, the daily administration dose is usually 1 to 2000mg, preferably 50 to 1000 mg. Of course, the particular dosage will depend upon such factors as the route of administration, the health of the patient, and the like, and is within the skill of the skilled practitioner.

The invention also provides a preparation method of the pharmaceutical composition, which comprises the following steps: mixing a pharmaceutically acceptable carrier with the compound of the general formula (I) or a crystal form, a pharmaceutically acceptable salt, a hydrate or a solvate thereof to form the pharmaceutical composition.

The present invention also provides a method of treatment comprising the steps of: administering a compound of formula (I), or a crystalline form, a pharmaceutically acceptable salt, hydrate, or solvate thereof, as described herein, or a pharmaceutical composition as described herein, to a subject in need of such treatment, for selectively inhibiting SOS 1.

Compared with the prior art, the invention has the following main advantages:

(1) the compound has good selective inhibition effect on SOS 1;

(2) the compound has better in vivo and in vitro pharmacodynamics and pharmacokinetic performance and lower toxic and side effects.

The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Experimental procedures without specific conditions noted in the following examples, molecular cloning is generally performed according to conventional conditions such as Sambrook et al: the conditions described in the Laboratory Manual (New York: Cold Spring Harbor Laboratory Press,1989), or according to the manufacturer's recommendations. Unless otherwise indicated, percentages and parts are by weight.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition, any methods and materials similar or equivalent to those described herein can be used in the methods of the present invention. The preferred embodiments and materials described herein are intended to be exemplary only.

The structure of the compounds of the invention is determined by Nuclear Magnetic Resonance (NMR) and liquid mass chromatography (LC-MS).

NMR was detected using a Bruker AVANCE-400 nuclear magnetic spectrometer, and the assay solvent contained deuterated dimethyl sulfoxide (DMSO-d) ₆ ) Deuterated acetone (CD) ₃ COCD ₃ ) DeuteriumChloroform (CDCl) ₃ ) And deuterated methanol (CD) ₃ OD), and internal standards are Tetramethylsilane (TMS), chemical shifts are measured in parts per million (ppm).

Liquid chromatography-mass spectrometry (LC-MS) was detected using a Waters SQD2 mass spectrometer. HPLC measurements were performed using an Agilent1100 high pressure chromatograph (Microsorb 5micron C18100x3.0mm column).

Thin layer chromatography silica gel plate is blue island GF254 silica gel plate, TLC is 0.15-0.20mm, and preparative thin layer chromatography is 0.4-0.5 mm. Column chromatography generally uses Qingdao silica gel 200-300 mesh silica gel as a carrier.

The starting materials in the examples of the present invention are known and commercially available or may be used or synthesized according to literature reports in the art.

All reactions of the present invention are carried out under continuous magnetic stirring under the protection of a dry inert gas (e.g., nitrogen or argon) except for the specific indications, and the reaction temperatures are all in degrees centigrade.

Examples

EXAMPLE 1 preparation of (R) -N- (1- (3- (3-amino-5- (trifluoromethyl) phenyl) ethyl) -2-methyl-7, 8-dihydro- [1,4] dioxins [2,3-g ] quinazolin-4-amine

The first step is as follows: preparation of 2, 3-dihydrobenzo [ b ] [1,4] dioxin-6-carbonitrile

3, 4-Dihydroxybenzonitrile (5g,37mmol) was added to N, N-dimethylformamide (50mL), and 1, 2-dibromoethane (28g,148mmol) and potassium carbonate (26g,185mmol) were added. The resulting reaction mixture was stirred at 80 ℃ overnight, then cooled and poured into 500mL of water, followed by extraction with 100 mL of ethyl acetate 3 times. The combined organic phases were dried and filtered, the filtrate was concentrated, and the obtained residue was separated by silica gel column chromatography (PE: EA ═ 5:1) to obtain the objective product (3.5g, yield: 58%).

LC-MS：m/z 162(M+H) ⁺ 。 ¹ H NMR(400MHz,CDCl ₃ )δ7.15-7.13(m,2H),6.92-6.90(m,1H),4.33-4.31(m,2H),4.29-4.27(m,2H).

The second step is that: preparation of 7-nitro-2, 3-dihydrobenzo [ b ] [1,4] dioxin-6-carbonitrile

2, 3-Dihydrobenzo [ b ] [1,4] dioxin-6-carbonitrile (1g,6.2mmol) was added to acetic anhydride (10mL), and concentrated nitric acid (1mL,15mmol) was added. The resulting reaction solution was stirred at room temperature overnight, then poured into 50mL of water, and extracted with 50mL of ethyl acetate. The organic phase was dried and filtered, and the filtrate was concentrated, and the obtained residue was separated by silica gel column chromatography (PE: EA ═ 10:1) to obtain the objective product (750mg, yield: 58%).

¹ H NMR(400MHz,CDCl ₃ )δ7.87(s,1H),7.33(s,1H),4.41(s,4H).

The third step is the preparation of 7-amino-2, 3-dihydrobenzo [ b ] [1,4] dioxin-6-carbonitrile

7-Nitro-2, 3-dihydrobenzo [ b ] [1,4] dioxin-6-carbonitrile (500mg) was added to methanol (5mL), followed by palladium on carbon (100 mg). The resulting reaction solution was stirred at room temperature for 3 hours under a hydrogen atmosphere, and then filtered. The filtrate was concentrated to give the objective product (420 mg). The product was used in the next reaction without further purification.

LC-MS：m/z 177(M+H) ⁺ 。

The fourth step is the preparation of N- (7-cyano-2, 3-dihydrobenzo [ b ] [1,4] dioxin-6-yl) acetamide

7-amino-2, 3-dihydrobenzo [ b ] [1,4] dioxin-6-carbonitrile (427mg) was added to methylene chloride (5mL), followed by acetic anhydride (5 mL). The obtained reaction solution was stirred at 70 ℃ overnight, and then cooled and concentrated to obtain the objective product (520 mg). The product was used in the next reaction without further purification.

LC-MS：m/z 219(M+H) ⁺ 。

The fifth step is the preparation of 2-methyl-7, 8-dihydro- [1,4] dioxine [2,3-g ] quinazolin-4-ol

N- (7-cyano-2, 3-dihydrobenzo [ b ] [1,4] dioxin-6-yl) acetamide (200mg,0.9mmol) was added to ethanol (2mL), followed by hydrogen peroxide (2mL) and sodium hydroxide (44mg,1.1 mmol). The resulting reaction solution was refluxed, stirred overnight, then cooled and concentrated. The residue was adjusted to pH 2 with 2N hydrochloric acid and then filtered. The solid was collected and dried to obtain the objective product (150mg, yield: 75%). The product was used in the next reaction without further purification.

LC-MS：m/z 219(M+H) ⁺ 。 ¹ H NMR(400MHz,DMSO-d ₆ )δ7.50(s,1H),7.21(s,1H),4.43-4.37(m,4H),2.53(s,3H).

The fifth step is the preparation of (R) -N- (1- (3- (3-amino-5- (trifluoromethyl) phenyl) ethyl) -2-methyl-7, 8-dihydro- [1,4] dioxins [2,3-g ] quinazolin-4-amine

2-methyl-7, 8-dihydro- [1,4] dioxine [2,3-g ] quinazolin-4-ol (150mg,0.7mmol) was added to N, N-dimethylformamide (2mL), followed by 1, 8-diazabicycloundecen-7-ene (310mg,2.1mmol), benzotriazol-1-yloxytris (dimethylamino) phosphonium hexafluorophosphate (610mg,1.4mmol), and (R) -3- (1-aminoethyl) -5- (trifluoromethyl) aniline (180mg,0.9 mmol). The resulting reaction mixture was stirred at 80 ℃ overnight, then cooled and poured into 50mL of water, followed by extraction with 20 mL of ethyl acetate 3 times. The combined organic phases were dried and filtered, the filtrate was concentrated, and the obtained residue was separated by preparative high performance liquid chromatography to give the objective product (43mg, yield: 15%).

LC-MS：m/z 405(M+H) ⁺ 。 ¹ H NMR(400MHz,DMSO-d ₆ )δ7.97(d,J＝8Hz,1H),7.90(s,1H),6.97(s,1H),6.88(s,1H),6.83(s,1H),6.68(s,1H),5.51(s,3H),4.34(d,J＝3.2Hz,4H),2.32(s,3H),1.51(d,J＝6.8Hz,3H).

The following compounds were synthesized according to the procedure of example 1, starting from different starting materials:

EXAMPLE 2 preparation of a mixture of N- ((R) -1- (3-amino-5- (trifluoromethyl) phenyl) ethyl) -7- (methoxymethyl) -2-methyl-7, 8-dihydro- [1,4] dioxane [2,3-g ] quinazolin-4-amine and N- ((R) -1- (3-amino-5- (trifluoromethyl) phenyl) ethyl) -8- (methoxymethyl) -2-methyl-7, 8-dihydro- [1,4] dioxane [2,3-g ] quinazolin-4-amine

LC-MS：m/z 449(M+H) ⁺ 。 ¹ H NMR(400MHz,DMSO-d ₆ )δ7.98-7.91(m,2H),6.99(s,1H),6.88(s,1H),6.83(s,1H),6.68(s,1H),5.52-5.47(m,3H),4.49-4.40(m,2H),4.15-4.09(m,1H),3.64-3.62(m,2H),3.34-3.33(m,3H),2.33(s,3H),1.52-1.50(m,3H).

Example 2 Manual isolation to four isomers

Example 2A

LC-MS：m/z 449(M+H) ⁺ 。 ¹ H NMR(400MHz,DMSO)δ7.96(d,J＝7.4Hz,2H),6.99(s,1H),6.88(s,1H),6.83(s,1H),6.68(s,1H),5.63–5.41(m,3H),4.45(t,J＝9.8Hz,2H),4.10(m,1H),3.67–3.53(m,2H),3.34(s,3H),2.33(s,3H),1.50(d,J＝7.0Hz,3H).

Example 2B

LC-MS：m/z 449(M+H) ⁺ 。 ¹ H NMR(400MHz,DMSO)δ8.02–7.87(m,2H),6.99(s,1H),6.88(s,1H),6.83(s,1H),6.68(s,1H),5.59–5.42(m,3H),4.43(d,J＝9.7Hz,2H),4.13(m,1H),3.64(t,J＝8.3Hz,2H),3.34(s,3H),2.33(s,3H),1.50(d,J＝7.0Hz,3H).

Example 2C

LC-MS：m/z 449(M+H) ⁺ 。 ¹ H NMR(400MHz,DMSO)δ7.98(d,J＝8.1Hz,1H),7.91(s,1H),6.99(s,1H),6.88(s,1H),6.83(s,1H),6.68(s,1H),5.50(m,3H),4.49(d,J＝4.9Hz,1H),4.41(dd,J＝11.6,2.2Hz,1H),4.10(m,1H),3.63(d,J＝4.8Hz,2H),3.33(s,3H),2.33(s,3H),1.51(d,J＝7.0Hz,3H).

Example 2D

LC-MS：m/z 449(M+H) ⁺ 。 ¹ H NMR(400MHz,DMSO)δ7.98(d,J＝8.1Hz,1H),7.91(s,1H),6.99(s,1H),6.88(s,1H),6.83(s,1H),6.70(d,J＝16.2Hz,1H),5.50(m,3H),4.49(d,J＝4.8Hz,1H),4.41(dd,J＝11.6,2.2Hz,1H),4.11(m,1H),3.63(d,J＝4.8Hz,2H),3.33(s,3H),2.33(s,3H),1.51(d,J＝7.0Hz,3H).

EXAMPLE 3 preparation of (R) -N- (1- (5-amino-2-fluoro-3- (trifluoromethyl) phenyl) ethyl) -2-methyl-7, 8-dihydro- [1,4] dioxane [2,3-g ] quinazolin-4-amine

LC-MS：m/z 423(M+H) ⁺ 。

EXAMPLE 4 preparation of (R) -N- (1- (2-fluoro-3- (trifluoromethyl) phenyl) ethyl) -2-methyl-7, 8-dihydro- [1,4] dioxane [2,3-g ] quinazolin-4-amine

LC-MS：m/z 408(M+H) ⁺ 。

EXAMPLE 5 preparation of (R) -1, 1-difluoro-1- (2-fluoro-3- (1- ((2-methyl-7, 8-dihydro- [1,4] dioxane [2,3-g ] quinazolin-4-yl) amino) ethyl) phenyl) -2-methylpropan-2-ol

LC-MS：m/z 448(M+H) ⁺ 。

EXAMPLE 6 preparation of (R) -2, 2-difluoro-2- (2-fluoro-3- (1- ((2-methyl-7, 8-dihydro- [1,4] dioxa [2,3-g ] quinazolin-4-yl) amino) ethyl) phenyl) ethan-1-ol

LC-MS：m/z 420(M+H) ⁺ 。

EXAMPLE 7 preparation of (R) -N- (1- (3-amino-5- (trifluoromethyl) phenyl) ethyl) -2-methyl-7, 8,10,11,13, 14-hexahydro- [1,4,7,10] tetraoxacyclododecane [2,3-g ] quinazolin-4-amine

LC-MS：m/z 493(M+H) ⁺ 。 ¹ H NMR(400MHz,CDCl ₃ )δ7.27(s,1H),7.16(s,1H),7.06(s,1H),6.88(s,1H),6.78(s,1H),5.59(m,2H),4.31–4.16(m,4H),3.96–3.70(m,10H),2.57(s,3H),1.62(d,J＝6.3Hz,3H).

Example 8(R) -N- (1- (3-amino-5- (trifluoromethyl) phenyl) ethyl) -6-methyl- [1,3] dioxolan [4,5-g ] quinazolin-8-amine

LC-MS：m/z 391(M+H) ⁺ 。

Example 9(R) -N- (1- (3-amino-5- (trifluoromethyl) phenyl) ethyl) -6-methyl- [1,3] dioxolan [4,5-g ] quinazoline-2, 2-d 2-8-amine

LC-MS：m/z 393(M+H) ⁺ 。

Example 10 evaluation of biological assay

The following biological test examples further illustrate the present invention, but these examples are not meant to limit the scope of the present invention.

Compound pair KRAS ^G12C Inhibition assay for binding to SOS 1.

Experimental procedure

(1) Gradient dilution of test compound: 10mM stock solution (dissolved in 100% DMSO) was added to 384 well assay plates to a final DMSO content of 0.25%.

(2) 5ul of Tag1-SOS1 solution was added to the test plate and 5ul of dilution buffer was added to the control.

(4) 5ul of Tag2-KRAS was added to the test plate ^G12C And (3) solution.

(4) 10ul of Anti-Tag1-Tb3+ and Anti-Tag2-XL665 test solution were added to the test plate. Centrifuged at 1000rpm for 1 min and incubated at room temperature for 2 hr.

(5) Reading board

(6) Finally, IC of the compound was calculated using GraphPad Prism software ₅₀ And (5) obtaining values, and drawing a fitting curve.

In the inventionCompound pair KRAS ^G12C The inhibitory activity of the enzyme in combination with SOS1 is shown in Table 1.

Table 1 inhibitory Activity of the Compounds of the examples of the present invention

IC ₅₀	(nM)
		Example 1	35
Example 2A	40.4
		Example 2B	42.3
Example 2C	23.1
		Example 2D	41.4
Example 7	4.1

As can be seen from table 1:

example Compounds of the invention for KRAS ^G12C The combination with SOS1 showed good inhibitory activity.

Pharmacokinetic testing evaluation

Male SD rats weighing about 220g were fasted overnight and then gavaged with 10mg/kg of a solution of the compound of the present invention [ CMC/TW80 as vehicle ]. Blood was collected at 0.5, 1.0, 2.0, 4.0, 6.0, 8.0, 12, and 24 post administration of the compound of the invention, respectively, and the concentration of the compound of the invention in plasma was determined by LC/MS/MS.

The detection result shows that the compound of the invention has good pharmacokinetic property.

Evaluation of antitumor Effect

100uL of the solution containing 5x10 ⁶ The MIA PaCa-2 tumor cell suspension was subcutaneously inoculated into the right posterior abdomen of nude mice. Mice were monitored daily for health and measurements were started when tumors grew to reach. The formula for calculating the tumor volume is 0.5xLxW ² Wherein L, W represents tumor length and width, respectively. Tumor growth to 150mm ³ Mice were randomly grouped. Mice were gavaged daily with the corresponding dose (15, 50mg/Kg) of CMC-Na suspension of the compound while monitoring their general state. Tumors were measured 3 times per week and body weights were measured twice per week.

The detection result shows that the compound has good anti-tumor effect.

All documents mentioned in this application are incorporated by reference in this application as if each were individually incorporated by reference. Furthermore, it should be understood that various changes and modifications of the present invention can be made by those skilled in the art after reading the above teachings of the present invention, and these equivalents also fall within the scope of the present invention as defined by the appended claims.

Claims

1. A compound of formula (I), a stereoisomer, a tautomer, a crystalline form, a pharmaceutically acceptable salt, a hydrate, a solvate, or a prodrug thereof:

in the formula:

are the same or different and are each independently aA bond or double bond;

R ¹ 、R ² the same or different, each independently selected from the group consisting of: hydrogen, deuterium, halogen, cyano, oxo, substituted or unsubstituted C ₂ -C ₁₈ Alkenyl, substituted or unsubstituted C ₂ -C ₁₈ Alkynyl, substituted or unsubstituted C ₁ -C ₁₈ Alkyl, substituted or unsubstituted C ₃ -C ₂₀ Cycloalkyl, substituted or unsubstituted 4-20 membered heterocycloalkyl, substituted or unsubstituted C ₁ -C ₁₈ Alkylene radical C ₃ -C ₂₀ Cycloalkylene, substituted or unsubstituted C ₁ -C ₁₈ Alkylene 4-20 membered heterocycloalkylene, - (CH) ₂ ) _h O(CH ₂ ) _p R ⁶ 、-(CH ₂ ) _h SR ⁶ 、-(CH ₂ ) _h COR ⁶ 、-(CH ₂ ) _h C(O)OR ⁶ 、-(CH ₂ ) _h S(O) _q R ⁶ 、-(CH ₂ ) _h NR ⁶ R ⁷ 、-(CH ₂ ) _h C(O)NR ⁶ R ⁷ 、-(CH ₂ ) _h NR ⁶ C(O)R ⁷ 、-(CH ₂ ) _h NR ⁶ C(O)NR ⁷ R ⁸ 、-(CH ₂ ) _h S(O) _q NR ⁶ R ⁷ 、-(CH ₂ ) _h NR ⁶ S(O) _q R ⁷ 、-(CH ₂ ) _h NR ⁶ S(O) _q NR ⁷ R ⁸ (ii) a Wherein, CH ₂ H in (a) may be optionally substituted; r is ⁶ 、R ⁷ And R ⁸ Each independently selected from the group consisting of: hydrogen, substituted or notSubstituted C ₁ -C ₁₈ Alkyl, substituted or unsubstituted C ₃ -C ₂₀ Cycloalkyl, substituted or unsubstituted 4-20 membered heterocyclyl, substituted or unsubstituted C ₆ -C ₁₄ Aryl and substituted or unsubstituted 5-14 membered heteroaryl; or in- (CH) ₂ ) _h NR ⁶ R ⁷ 、-(CH ₂ ) _h C(O)NR ⁶ R ⁷ 、-(CH ₂ ) _h S(O) _q NR ⁶ R ⁷ In, R ⁶ And R ⁷ And the adjacent N atom thereof form a substituted or unsubstituted 4-8 membered heterocyclic group; or in- (CH) ₂ ) _h NR ⁶ C(O)R ⁷ 、-(CH ₂ ) _h NR ⁶ C(O)NR ⁷ R ⁸ 、-(CH ₂ ) _h NR ⁶ S(O) _q R ⁷ 、-(CH ₂ ) _h NR ⁶ S(O) _q NR ⁷ R ⁸ In, R ⁷ And R ⁸ Is cyclized with its adjacent N atom to form a substituted or unsubstituted 4-8 membered heterocyclic group, or R ⁶ And R ⁷ Ring-closing with its adjacent atom to form a substituted or unsubstituted 4-8 membered heterocyclic group;

m is 0,1, 2,3 or 4;

n is 0,1, 2,3, 4 or 5;

h is 0,1, 2,3, 4,5 or 6;

p is 0,1, 2,3, 4,5 or 6;

q is 1 or 2.

2. The compound, its stereoisomer, tautomer, crystal form, pharmaceutically acceptable salt, hydrate, solvate, or prodrug of claim 1, having the structure of formula (II):

wherein, ring A, ring B, R ¹ 、R ² 、R ³ 、R ⁴ 、R ⁵ M and n are as defined in claim 1.

3. The compound, its stereoisomers, tautomers, crystal forms, pharmaceutically acceptable salts, hydrates, solvates or prodrugs thereof according to claim 1, having the structure of formula (III):

wherein, ring A, ring B, R ¹ 、R ² 、R ³ 、R ⁴ M and n are as defined in claim 1.

4. The compound, stereoisomer, tautomer, crystal form, pharmaceutically acceptable salt, hydrate, solvate or prodrug thereof according to claim 1, characterized in that it has the structure according to formula (IV):

in the formula: r ¹ 、R ² 、R ³ Ring a, ring B, m, and n are as defined in claim 1.

5. The compound, stereoisomer, tautomer, crystal form, pharmaceutically acceptable salt, hydrate, solvate or prodrug thereof according to claim 1, characterized in that it has the structure according to formula (V):

in the formula:

R ¹ 、R ² 、R ³ ring B, m and n are as defined in claim 1.

6. The compound, stereoisomer, tautomer, crystalline form, pharmaceutically acceptable salt, hydrate, solvate or prodrug thereof according to any one of claims 1 to 5, characterized in that it has the structure according to formula (VI):

in the formula (I), the compound is shown in the specification,

R ¹ 、R ² 、R ³ ring B, m, and n are as defined in claim 1, R ^y Is defined as in claim 5.

7. The compound, its stereoisomers, tautomers, crystalline forms, pharmaceutically acceptable salts, hydrates, solvates or prodrugs thereof according to any one of claims 1-6, wherein ring B is selected from the group consisting of substituted or unsubstituted: c ₃ -C ₁₀ Cycloalkyl, 4-16 membered heterocyclyl, phenyl and 5-6 membered heteroaryl, wherein said substitution means substitution by one or more groups selected from the group consisting of: c ₁ -C ₆ Alkyl, deuterated C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl, halo C ₁ -C ₆ Alkyl hydroxy, C ₃ -C ₆ Cycloalkyl radical, C ₁ -C ₆ Alkoxy, deuterated C ₁ -C ₆ Alkoxy, halo C ₁ -C ₆ Alkoxy, halogen, nitro, hydroxyl, oxo, cyano, ester, amine, amide, sulfonamide, or urea.

8. The compound, stereoisomer, tautomer, crystalline form, pharmaceutically acceptable salt, hydrate, solvate or prodrug thereof according to any one of claims 1 to 7, characterized in that said compound is selected from the group consisting of:

9. a pharmaceutical composition comprising i) one or more compounds of any one of claims 1-8, stereoisomers, tautomers, crystalline forms, pharmaceutically acceptable salts, hydrates, solvates, or prodrugs thereof; and ii) a pharmaceutically acceptable carrier.

10. Use of a compound according to any one of claims 1 to 8, a stereoisomer, a tautomer, a crystalline form, a pharmaceutically acceptable salt, a hydrate, a solvate, or a prodrug thereof, or a pharmaceutical composition according to claim 9, for the preparation of a pharmaceutical composition for the prophylaxis and/or treatment of a disease associated with the activity or expression of SOS 1.