CN114796270B - Wula grass nano silver, preparation and application thereof, nano silver gel and preparation and application thereof - Google Patents
Wula grass nano silver, preparation and application thereof, nano silver gel and preparation and application thereof Download PDFInfo
- Publication number
- CN114796270B CN114796270B CN202210250958.0A CN202210250958A CN114796270B CN 114796270 B CN114796270 B CN 114796270B CN 202210250958 A CN202210250958 A CN 202210250958A CN 114796270 B CN114796270 B CN 114796270B
- Authority
- CN
- China
- Prior art keywords
- silver
- nano
- nano silver
- preparation
- uraria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/38—Silver; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/89—Cyperaceae (Sedge family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/46—Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides a black-grass nano silver, a preparation method and an application thereof, a nano silver gel, and a preparation method and an application thereof, and belongs to the technical field of medical materials. According to the invention, the wula sedge is used as a stabilizer and a reducing agent, and contains active ingredients such as polysaccharide, flavone and alkaloid, so that silver nano-particles are formed, and the wula sedge splits and wraps the nano-silver particles in a reducing process, so that nano-silver agglomeration is prevented, the formed wula sedge nano-silver has good stability in a solution, and the formed wula sedge nano-silver can be kept from being obviously aggregated within two months; the prepared Wula grass nano silver has excellent antibacterial activity on gram-positive bacteria and gram-negative bacteria, can avoid the use of antibiotics, and has wide prospect in the aspect of wound healing.
Description
Technical Field
The invention relates to the technical field of medical materials, in particular to glaucescent fissistigma root nano silver, preparation and application thereof, nano silver gel and preparation and application thereof.
Background
The nano silver has the advantages of unique optical, electronic, catalytic, mechanical and other characteristics, good antibacterial activity, biocompatibility, easy surface modification and the like, becomes the most important and commercialized nano material, and is generally applied to the fields of biological pesticides, novel biological sensors, water purification and the like.
The skin acts as a protective barrier between the external environment and the human body, and excessive heat and supercooling can destroy the skin barrier, resulting in trauma. Under natural conditions, the skin has some ability to repair itself, but slow repair processes can cause the skin to enter an inflammatory state. Therefore, it is highly necessary to promote wound healing by means of external forces. The wound treatment requires a slightly moist environment, and the hydrogel is a net-like polymer having excellent water absorption and retention properties, and the gel swells in the presence of water, the degree of swelling being related to the ratio of hydrophobic and hydrophilic groups. In addition, the hydrogel has good elasticity and biocompatibility, can give a moist environment to the wound, and is beneficial to the healing of the wound. However, the moist environment of hydrogels can lead to bacterial growth, and the current approach to inhibit bacterial growth is to add antibiotics, but antibiotic treatment methods can develop resistance.
Disclosure of Invention
The invention aims to provide uraria nano silver, preparation and application thereof, nano silver gel and preparation and application thereof, wherein the uraria nano silver has excellent antibacterial activity, can be prepared into antibacterial gel, and avoids the use of antibiotics.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a preparation method of wula sedge nano silver, which comprises the following steps:
mixing the wula sedge powder, silver salt and water, and carrying out reduction reaction on the obtained mixed solution to obtain the wula sedge nano silver.
Preferably, the concentration of the uraria powder in the mixed solution is 0.5-2 mg/mL, and the concentration of the silver salt in the mixed solution is 0.1-1 mmol/L.
Preferably, the temperature of the reduction reaction is 50-90 ℃ and the time is 1-4 h.
The invention provides the black-bone grass nano silver prepared by the preparation method of the technical scheme, which comprises nano silver particles and black-bone grass extract coated on the surfaces of the nano silver particles; the average particle diameter of the nano silver particles is 64.17nm.
The invention provides application of the uraria nano-silver in antibacterial materials.
The invention provides a nano silver gel which is prepared from uraria nano silver, wherein the uraria nano silver is prepared from uraria nano silver according to the technical scheme.
The invention provides a preparation method of the nano silver gel, which comprises the following steps:
heating and mixing the adhesive aqueous solution and the uraria nano-silver, and repeatedly freezing and thawing to obtain the nano-silver gel, wherein the uraria nano-silver is prepared by the technical scheme.
Preferably, the binder in the aqueous binder solution comprises polyvinyl alcohol; the mass concentration of the adhesive aqueous solution is 7-10%.
Preferably, the content of the uraria nanometer silver in the nanometer silver gel is 4-8 mg/kg.
The invention provides application of the nano silver gel prepared by the technical scheme or the preparation method of the technical scheme in preparing a wound healing material.
The invention provides a preparation method of wula sedge nano silver, which comprises the following steps: mixing the wula sedge powder, silver salt and water, and carrying out reduction reaction on the obtained mixed solution to obtain the wula sedge nano silver. According to the invention, the wula sedge is used as a stabilizer and a reducing agent, and contains active ingredients such as polysaccharide, flavone and alkaloid, so that silver nano-particles are formed, and the wula sedge splits and wraps the nano-silver particles in a reducing process, so that nano-silver agglomeration is prevented, the formed wula sedge nano-silver has good stability in a solution, and the formed wula sedge nano-silver can be kept from being obviously aggregated within two months; the prepared Wula grass nano silver has excellent antibacterial activity on gram-positive bacteria and gram-negative bacteria, can avoid the use of antibiotics, and has wide prospect in the aspect of wound healing.
The invention provides a preparation method of wula sedge nano silver, which takes wula sedge as a reducing agent, and the wula sedge extract synthetic material has low price, wide sources, safety, environmental protection and high synthetic speed.
The prepared wula sedge nano silver is prepared into nano silver gel, and the nano silver gel has the advantages of stability and difficulty in generating drug resistance, and can freely enter cell membranes of bacteria at a very low concentration due to small nano silver particle size (average particle size is 64.17 nm), and the nano silver particles and the cell membranes have very strong binding capacity to lose activity, so that the nano silver gel can well inhibit the growth of bacteria and viruses, does not generate drug resistance and has very good market application prospect.
Drawings
FIG. 1 is a transmission electron microscope image of the Wula sedge nano-silver prepared in example 10;
FIG. 2 is an ultraviolet-visible absorption spectrum of the wula sedge nano-silver prepared in examples 1 to 4 and the product of comparative example 1;
FIG. 3 is an ultraviolet-visible absorption spectrum of the wula sedge nano-silver prepared in examples 4 to 6 and the product of comparative example 2;
FIG. 4 is a photograph of the wula sedge nano silver solution prepared in example 10;
FIG. 5 is an ultraviolet-visible absorption spectrum of the Wula-grass nano-silver prepared in examples 4 and 7-9;
FIG. 6 is a photograph of the wula sedge nano silver solution prepared in example 10 left at room temperature for two months;
FIG. 7 is an ultraviolet-visible absorption spectrum of the wula sedge nano-silver prepared in examples 4, 10-11 and the products of comparative examples 3-4;
FIG. 8 is a graph showing the diameter of the inhibition zone of the prepared wula sedge nano-silver and wula sedge powder of example 10;
FIG. 9 shows the results of the antibacterial rate of the nano silver of Wula grass prepared in example 10 and the nano silver gel prepared in example 12;
FIG. 10 is a Markov particle size analysis result of the wula sedge nano silver prepared in example 10;
FIG. 11 is a Zate potential diagram of the wula sedge nano silver prepared in example 10.
Detailed Description
The invention provides a preparation method of wula sedge nano silver, which comprises the following steps:
mixing the wula sedge powder, silver salt and water, and carrying out reduction reaction on the obtained mixed solution to obtain the wula sedge nano silver.
In the present invention, the preparation materials are commercially available as known to those skilled in the art unless otherwise specified.
The source of the wula sedge powder is not particularly limited, and the wula sedge powder is commercially available and well known in the art.
In the present invention, the silver salt preferably includes silver nitrate; the concentration of the silver salt in the mixed solution is preferably 0.1 to 1mmol/L, more preferably 0.5mmol/L.
In the invention, the concentration of the uraria powder in the mixed solution is preferably 0.5-2 mg/mL, more preferably 1.0-1.5 mg/mL; according to the invention, the wula sedge is used as a stabilizer and a reducer, and contains active ingredients such as polysaccharide, flavone, alkaloid and the like, so that nano-particles are formed, and nano-silver particles are wrapped by wula sedge through self-splitting, so that nano-silver agglomeration is prevented, and the nano-silver has better stability.
The process of mixing the wula sedge powder, the silver salt and the water is not particularly limited, and the materials are uniformly mixed according to the process well known in the art, for example, the wula sedge powder is dissolved in the water and the silver salt is added, or the wula sedge powder and the silver salt are respectively dissolved in the water and then mixed.
In the present invention, the temperature of the reduction reaction is preferably 50 to 90 ℃, more preferably 60 to 80 ℃, still more preferably 70 ℃; the time is preferably 1 to 4 hours, more preferably 60 to 90 minutes, still more preferably 70 to 80 minutes.
After the reduction reaction is completed, the method is preferably used for directly obtaining the Wula grass nano-silver solution without any treatment. The uraria nano silver exists in a solution form; the concentration of the uraria nano silver solution is not particularly limited, and the uraria nano silver solution can be adjusted according to actual requirements.
The invention provides the black-bone grass nano silver prepared by the preparation method of the technical scheme, which comprises nano silver particles and black-bone grass extract coated on the surfaces of the nano silver particles; the average particle diameter of the nano silver particles is 64.17nm.
The invention provides application of the uraria nano-silver in antibacterial materials.
The invention provides a nano silver gel which is prepared from uraria nano silver, wherein the uraria nano silver is prepared from uraria nano silver according to the technical scheme.
The invention provides a preparation method of the nano silver gel, which comprises the following steps:
heating and mixing the adhesive aqueous solution and the uraria nano-silver, and repeatedly freezing and thawing to obtain the nano-silver gel, wherein the uraria nano-silver is prepared by the technical scheme.
In the present invention, the binder in the aqueous binder solution preferably includes polyvinyl alcohol; the mass concentration of the aqueous binder solution is preferably 7 to 10%. The invention uses the adhesive to play the roles of adhering gel and preserving moisture.
In the invention, the heating and mixing process of the adhesive aqueous solution and the uraria nano-silver is preferably to heat the adhesive solution for 5 hours, then add the uraria nano-silver solution, and continuously heat and stir for 30 minutes; the heating temperature is preferably 80-90 ℃; the invention utilizes the heating process to improve the reaction rate, and the silver nitrate and the uraria plant powder fully react.
The present invention is not particularly limited to the freeze-thawing process, and a gel may be obtained according to a process well known in the art; in the examples of the present invention, the number of times of repeated freeze-thawing was three times, frozen in a refrigerator at-18℃for 2 hours.
In the invention, the content of the uraria nano silver in the nano silver gel is preferably 4-8 mg/kg, more preferably 8mg/kg; the dosage of the adhesive aqueous solution and the uraria nano silver can meet the content range of the uraria nano silver.
The invention provides application of the nano silver gel prepared by the technical scheme or the preparation method of the technical scheme in preparing a wound healing material. The method of application of the present invention is not particularly limited, and may be applied according to methods well known in the art. The nano silver gel prevents wound infection by inhibiting pathogenic microorganisms.
The technical solutions of the present invention will be clearly and completely described in the following in connection with the embodiments of the present invention. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Dissolving Lawsonia inermis powder in water to form Lawsonia inermis solution with concentration of 0.5mg/mL, adding AgNO 1mM 3 And (3) carrying out a reduction reaction on the aqueous solution at 80 ℃ for 90min to obtain the wula sedge nano silver solution.
Example 2
The only difference from example 1 is that: the concentration of the uraria solution is 1mg/mL.
Example 3
The only difference from example 1 is that: the concentration of the uraria solution is 1.5mg/mL.
Example 4
The only difference from example 1 is that: the concentration of the uraria solution is 2mg/mL.
Example 5
To a solution of Ula plant at a concentration of 2mg/mL, 0.1mM AgNO was added 3 Aqueous solution at 80Reacting at the temperature of 80min to obtain the Wula grass nano silver solution.
Example 6
The only difference from example 5 is that: agNO 3 The concentration of the aqueous solution was 0.5mM.
Example 7
To a solution of Ula grass at a concentration of 2mg/mL was added 1mM AgNO 3 And (3) carrying out a reduction reaction on the aqueous solution at 70 ℃ for 90min to obtain the wula sedge nano-silver solution.
Example 8
The only difference from example 7 is that: the temperature of the reduction reaction was 60 ℃.
Example 9
The only difference from example 7 is that: the temperature of the reduction reaction was 50 ℃.
Example 10
To a solution of Ula grass at a concentration of 2mg/mL was added 1mM AgNO 3 And (3) carrying out reduction reaction on the aqueous solution at 80 ℃ for 80min to obtain the wula sedge nano silver solution.
Example 11
The only difference from example 10 is that: the time for the reduction reaction was 60min.
Example 12
Preparing 10% polyvinyl alcohol aqueous solution, heating at 90 ℃ for 5 hours, adding the glaucescent fissistigma root nano silver solution prepared in the example 10, continuously stirring for 30 minutes, standing at room temperature, freezing for 2 hours in a refrigerator at-18 ℃, thawing, and repeatedly freezing and thawing for three times to obtain nano silver gel, wherein the content of glaucescent fissistigma root nano silver in the nano silver gel is 8mg/kg.
Comparative example 1
The only difference from example 1 is that: the concentration of the uraria solution is 0mg/mL.
Comparative example 2
The only difference from example 5 is that: agNO 3 The concentration of the aqueous solution was 0mM.
Comparative example 3
The only difference from example 10 is that: the time for the reduction reaction was 40min.
Comparative example 4
The only difference from example 10 is that: the time for the reduction reaction was 20min.
Characterization and performance testing
1) The result of transmission electron microscope test of the black-grass nano silver prepared in example 10 is shown in fig. 1; as can be seen from FIG. 1, the silver nanoparticles were mostly spherical, with a size between 37 and 122nm and an average particle diameter of 64.17nm.
2) Ultraviolet testing is carried out on the uraria nano-silver prepared in examples 1-4 and the product of comparative example 1, and the obtained ultraviolet visible absorption spectrum is shown in figure 2; as can be seen from fig. 2, the sample of comparative example 1, to which no wula sedge was added, had no absorption peak; the addition of the uraria at different concentrations can generate obvious absorption peaks, which proves that the uraria can induce the formation of nano silver.
3) Ultraviolet testing is carried out on the wula sedge nano silver prepared in examples 4-6 and the product of comparative example 2, and the obtained ultraviolet visible absorption spectrum is shown in figure 3; as can be seen from FIG. 3, agNO was added at various concentrations 3 Absorption peaks were observed, demonstrating AgNO at 0.1mM, 0.5mM, and 1mM 3 The aqueous solution can synthesize nano silver.
4) Fig. 4 is a photograph of the nano silver solution of wula sedge prepared in example 10, which is reddish brown, illustrating the synthesis of nano silver.
5) Ultraviolet testing is carried out on the uraria nano silver prepared in the examples 4 and 7-9, and the obtained ultraviolet visible absorption spectrum is shown in figure 5; as can be seen from fig. 5, absorption peaks appear at different temperatures, indicating that nano silver can be generated at different temperatures.
6) Fig. 6 is a photograph of the nano silver solution of wula sedge prepared in example 10, which was left at room temperature for two months, and as can be seen from fig. 6, the nano silver solution of wula sedge is reddish brown, and the synthesized nano silver can be kept stable, and no significant agglomeration occurs for two months at room temperature.
7) Ultraviolet testing is carried out on the wula sedge nano silver prepared in the examples 4 and 10-11 and the products of the comparative examples 3-4, and the obtained ultraviolet visible absorption spectrum is shown in figure 7; as can be seen from fig. 7, as the reaction time is prolonged, the ultraviolet absorbance of the nano silver is continuously increased, and the nano silver has no significant change after 90min, which indicates that the synthesis amount of the nano silver is maximum at 90 min.
8) Biological activity of a, nano silver
The antibacterial activity of the prepared wula sedge nano silver of example 10 on the selected strain was measured by using escherichia coli and bacillus subtilis as pathogen models and using a paper sheet diffusion method (DDM): commercial LB agar medium is sterilized at 121 ℃ for 15min under high pressure, the test strain is inoculated on the medium for culturing for 12h, the culture solution is taken for antibacterial detection, finally, a inhibition zone is observed by a microscope, three or more antibacterial activity detection is carried out, and the result is shown in figure 8 by comparing with Wula grass powder. As shown in fig. 8, on the solid medium, the nano silver particles of the wula sedge have remarkable antibacterial effects on gram-positive bacteria (bacillus subtilis) and gram-negative bacteria (escherichia coli), and the sizes of antibacterial rings of the nano silver particles of the wula sedge on the escherichia coli and the bacillus subtilis are respectively 10.48+/-1 mm and 11.59 +/-0.25 mm; whereas wula sedge powder does not have a zone of inhibition, indicating no antibacterial ability.
b. Biological Activity of nanosilver gel
Coli and bacillus subtilis were inoculated into LB medium and placed on a shaker at 37 ℃ for 12 hours. Subsequently diluting the two bacteria to 108CFU/mL with a culture medium; the nano silver gel or the uraria nano silver with the diameter of 1cm is placed in a conical flask, cultured for 6 hours at 37 ℃, 100 mu L of the gel-treated bacterial solution is taken and coated on LB solid culture medium, placed in a 37 ℃ incubator, cultured for 12 hours, the growth condition of the strain is observed, and the strain is compared with polyvinyl alcohol hydrogel, and the obtained result is shown in figure 9. Fig. 9 shows the results of the antibacterial rate of the nano silver of the wula sedge prepared in example 10 and the nano silver gel prepared in example 12 on bacillus subtilis and escherichia coli, and as can be seen from fig. 9, the antibacterial rate of the nano silver of the wula sedge and the nano silver gel on gram-positive bacteria and gram-negative bacteria can reach 99.9%, which shows that the nano silver gel has an obvious effect of inhibiting the growth of bacteria.
c. The particle size of the wula sedge nano silver prepared in example 10 was detected by using a malvern particle sizer, and the experimental result is shown in fig. 10; as is clear from FIG. 10, 1mM of the prepared wula sedge nano silver having an average particle size of 64.17nm and 25% of the wula sedge nano silver having a particle size distribution of 60nm was measured by a Markov particle size analyzer.
d. The result of the potential analysis of the wula sedge nano silver prepared in example 10 is shown in fig. 11, and it is apparent from fig. 11 that the potential analysis is at-19.2 mV, indicating that the nano silver particles have excellent stability.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (9)
1. A preparation method of wula sedge nano silver comprises the following steps:
mixing the wula sedge powder, silver salt and water, and carrying out reduction reaction on the obtained mixed solution to obtain wula sedge nano silver;
the concentration of the uraria powder in the mixed solution is 0.5-2 mg/mL, and the concentration of the silver salt in the mixed solution is 0.1-1 mmol/L.
2. The method according to claim 1, wherein the reduction reaction is carried out at a temperature of 50 to 90 ℃ for a time of 1 to 4 hours.
3. The wula sedge nano silver prepared by the preparation method of any one of claims 1-2, which comprises nano silver particles and wula sedge extract coated on the surfaces of the nano silver particles; the average particle diameter of the nano silver particles is 64.17nm.
4. Use of the uraria nanosilver of claim 3 in the preparation of a bacteriostatic material.
5. A nano silver gel, which is characterized by being prepared from uraria nano silver, wherein the uraria nano silver is the uraria nano silver in claim 3.
6. The method for preparing nano-silver gel according to claim 5, comprising the steps of:
heating and mixing the adhesive aqueous solution and the uraria nano-silver, and repeatedly freezing and thawing to obtain the nano-silver gel, wherein the uraria nano-silver is the uraria nano-silver according to claim 4.
7. The method of claim 6, wherein the binder in the aqueous binder solution comprises polyvinyl alcohol; the mass concentration of the adhesive aqueous solution is 7-10%.
8. The preparation method of claim 6, wherein the content of the wula sedge nano silver in the nano silver gel is 4-8 mg/kg.
9. Use of the nano-silver gel according to claim 5 or the nano-silver gel prepared by the preparation method according to any one of claims 6 to 8 in the preparation of a material for treating wound healing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210250958.0A CN114796270B (en) | 2022-03-15 | 2022-03-15 | Wula grass nano silver, preparation and application thereof, nano silver gel and preparation and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210250958.0A CN114796270B (en) | 2022-03-15 | 2022-03-15 | Wula grass nano silver, preparation and application thereof, nano silver gel and preparation and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114796270A CN114796270A (en) | 2022-07-29 |
| CN114796270B true CN114796270B (en) | 2023-04-25 |
Family
ID=82528315
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210250958.0A Active CN114796270B (en) | 2022-03-15 | 2022-03-15 | Wula grass nano silver, preparation and application thereof, nano silver gel and preparation and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114796270B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115282329A (en) * | 2022-08-19 | 2022-11-04 | 河北农业大学 | Antibacterial repair material and preparation method and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101305983A (en) * | 2008-05-26 | 2008-11-19 | 钱爱民 | Nanometer Ag antibiotic gent for women and its preparation method |
| CN101305737A (en) * | 2008-07-11 | 2008-11-19 | 刘柏生 | Preparation method of nano-silver sterilization gel |
| CN112998351B (en) * | 2021-02-23 | 2022-09-20 | 温州天望电子科技有限公司 | Meyer sedge health-care bacteriostatic shoes |
-
2022
- 2022-03-15 CN CN202210250958.0A patent/CN114796270B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN114796270A (en) | 2022-07-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Vimala et al. | Fabrication of curcumin encapsulated chitosan-PVA silver nanocomposite films for improved antimicrobial activity | |
| Elbarbary et al. | Radiation synthesis and characterization of polyvinyl alcohol/chitosan/silver nanocomposite membranes: antimicrobial and blood compatibility studies | |
| Mohamed et al. | Synthesis and characterization of antimicrobial crosslinked carboxymethyl chitosan nanoparticles loaded with silver | |
| CN108042565B (en) | Black phosphorus nano material with antibacterial effect and preparation method thereof | |
| Dacrory et al. | Preparation and characterization of eco-friendly carboxymethyl cellulose antimicrobial nanocomposite hydrogels | |
| Dai et al. | High antibacterial activity of chitosan films with covalent organic frameworks immobilized silver nanoparticles | |
| CN111202091A (en) | Nano-silver loaded mesoporous silica antibacterial material and preparation method and application thereof | |
| Anagha et al. | Biomass derived antimicrobial hybrid cellulose hydrogel with green ZnO nanoparticles for curcumin delivery and its kinetic modelling | |
| Silva et al. | In vitro activity of water-soluble quaternary chitosan chloride salt against E. coli | |
| CN107281541A (en) | A kind of preparation method of medical temperature-sensitive hydrogel | |
| Walke et al. | Physicochemical and functional characterization of chitosan prepared from shrimp shells and investigation of its antibacterial, antioxidant and tetanus toxoid entrapment efficiency | |
| CN107398562B (en) | The preparation method of fulvic acid nano silver gel | |
| CN114796270B (en) | Wula grass nano silver, preparation and application thereof, nano silver gel and preparation and application thereof | |
| CN113773525B (en) | Preparation method and application of molybdenum disulfide-loaded nano-silver antibacterial hydrogel | |
| Yang et al. | Antibacterial aerogels with nano‑silver reduced in situ by carboxymethyl cellulose for fresh meat preservation | |
| Li et al. | Ecofriendly synthesis of silver–carboxy methyl cellulose nanocomposites and their antibacterial activity | |
| Chen et al. | Highly absorbent antibacterial chitosan-based aerogels for shelf-life extension of fresh pork | |
| Zhu et al. | Preparation, characterization, and antibacterial effect of bio-based modified starch films | |
| Khalil et al. | Bimetallic hydrogels based on chitosan and carrageenan as promising materials for biological applications | |
| CN108213459B (en) | Preparation method of glucan/nano gold-silver alloy compound | |
| RU2474471C2 (en) | Colloidal solution of silver nanoparticles, metal-polymer nanocomposite film material, methods for production thereof, bactericidal composition based on colloidal solution and bactericidal film made from metal-polymer material | |
| Wang et al. | κ− Carrageenan− Based flexible antibacterial and pH− Sensitive hydrogels with phenanthroline covalent conjugation groups | |
| CN101407586B (en) | Nano silver / dextran gel hybridization material, and preparation and use thereof | |
| CN109988255B (en) | Preparation method and application of water-soluble thiolated chitosan | |
| CN105878280B (en) | Preparation method with the medical temperature-sensitive hydrogel of bacteriostasis efficacy |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Yan Inventor after: Zhou Xinyu Inventor after: Zhu Henan Inventor after: Shen Qihui Inventor after: Jiang Tienan Inventor after: Lv Mo Inventor after: Chen Yuxi Inventor after: Zhu Zihao Inventor after: Cai Yanyan Inventor after: Han Yutong Inventor before: Zhu Henan Inventor before: Liu Yan Inventor before: Jiang Tienan Inventor before: Lv Mo Inventor before: Chen Yuxi Inventor before: Zhu Zihao Inventor before: Cai Yanyan Inventor before: Han Yutong Inventor before: Zhou Xinyu Inventor before: Shen Qihui |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |