CN114794025A - Low-oxygen stress test device and method for grain storage insects - Google Patents
Low-oxygen stress test device and method for grain storage insects Download PDFInfo
- Publication number
- CN114794025A CN114794025A CN202210486115.0A CN202210486115A CN114794025A CN 114794025 A CN114794025 A CN 114794025A CN 202210486115 A CN202210486115 A CN 202210486115A CN 114794025 A CN114794025 A CN 114794025A
- Authority
- CN
- China
- Prior art keywords
- box
- observation
- box body
- control mechanism
- fixedly connected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000012360 testing method Methods 0.000 title claims abstract description 89
- 241000238631 Hexapoda Species 0.000 title claims abstract description 69
- 238000003860 storage Methods 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 17
- 239000001301 oxygen Substances 0.000 title claims abstract description 15
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 15
- 239000007789 gas Substances 0.000 claims abstract description 60
- 230000007246 mechanism Effects 0.000 claims abstract description 60
- 238000003825 pressing Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 206010021143 Hypoxia Diseases 0.000 claims description 20
- 230000007954 hypoxia Effects 0.000 claims description 17
- 230000005540 biological transmission Effects 0.000 claims description 12
- 238000002347 injection Methods 0.000 claims description 10
- 239000007924 injection Substances 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 10
- 229920006395 saturated elastomer Polymers 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 3
- 239000012266 salt solution Substances 0.000 claims description 2
- 241000607479 Yersinia pestis Species 0.000 abstract description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 4
- 238000004320 controlled atmosphere Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000006978 adaptation Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 208000018875 hypoxemia Diseases 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000001146 hypoxic effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/51—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture specially adapted for storing agricultural or horticultural products
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Testing Resistance To Weather, Investigating Materials By Mechanical Methods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a device and a method for testing low oxygen stress of grain storage insects, and the device comprises a test box body, wherein a transverse plate is fixedly connected in the test box body, the transverse plate divides the test box body into a treatment cavity and an operation cavity, the treatment cavity is positioned above the operation cavity, a plurality of through holes are formed in the transverse plate, the treatment cavity is communicated with the operation cavity through the through holes, a plurality of insect observation mechanisms are arranged in the treatment cavity, the insect observation mechanisms are respectively arranged corresponding to the through holes, a humidity control mechanism, a gas control mechanism and a pressure control mechanism are arranged on the test box body, the humidity control mechanism, the gas control mechanism and the pressure control mechanism are all arranged corresponding to the interior of the test box body, a box cover is hinged to the top end of the test box body, a pressing mechanism is arranged on the box cover, and the box cover is in limit fit with the test box body through the pressing mechanism. The method is suitable for researching the reaction of the grain storage pests to the low-oxygen environment under the conditions of different humidity and gas concentration under the normal pressure condition, and the samples can be stored and examined at any time, so that the low-oxygen stress test of the grain storage pests is facilitated.
Description
Technical Field
The invention relates to the technical field of insect tests, in particular to a device and a method for testing low oxygen stress of grain storage insects.
Background
The pest in stored grain is one of the important factors harming the safety of stored grain in China, and the low-oxygen controlled atmosphere technology is used for preventing and controlling the pest in stored grain, so that the pest in stored grain is gradually popularized and applied in China, but if the controlled atmosphere fails, great adverse effects are caused, the pest in stored grain is lost, the pest in stored grain generates controlled atmosphere resistance, and the difficulty of controlled atmosphere application is increased. When storing up grain insect hypoxemia among the prior art and coercing the experiment, sample access inspection needs switching device many times, and the sample is taken inconveniently, influences experimental efficiency, is not convenient for store up grain insect hypoxemia and coerces experimental insect sample and observes, and insect stress is great when experimental simultaneously, influences the accuracy of experiment. Therefore, a device and a method for testing the hypoxia stress of grain storage insects are needed to test and research the viability of different grain storage pests in a hypoxic environment.
Disclosure of Invention
The invention aims to provide a device and a method for testing hypoxia stress of grain storage insects, which are used for solving the problems in the prior art.
In order to achieve the purpose, the invention provides the following scheme: the invention provides a low oxygen stress test device for grain storage insects, which comprises a test box body, wherein a transverse plate is fixedly connected in the test box body, the transverse plate divides the test box body into a processing cavity and an operation cavity, the processing cavity is positioned above the operation cavity, the transverse plate is provided with a plurality of through holes, the processing cavity is communicated with the operation cavity through the through holes, a plurality of insect observation mechanisms are arranged in the treatment cavity and respectively correspond to the through holes, the test box body is provided with a humidity control mechanism, a gas control mechanism and a pressure control mechanism, the humidity control mechanism, the gas control mechanism and the pressure control mechanism are all arranged corresponding to the interior of the test box body, the top end of the test box body is hinged with a box cover, a pressing mechanism is arranged on the box cover, and the box cover is in limit fit with the test box body through the pressing mechanism.
Preferably, insect observation mechanism includes fixed connection and is in disc on the treatment chamber inner wall, annular spout has been seted up to the disc front end, the central point of annular spout with the crisscross setting of central point of disc, the middle part of disc is rotated and is connected with the transmission shaft, transmission shaft one end is stretched out the outer fixedly connected with crank of test box, transmission shaft other end fixedly connected with holds carrier, hold carrier with annular spout corresponds the setting, it places the frame to be provided with a plurality of observation boxes on holding carrier, it is connected with the observation box to dismantle on placing the frame to observe the box, observe the box with the through-hole corresponds the setting.
Preferably, hold carrier and include fixed connection and be in epaxial the dish that bears of transmission, bear a set central point with the coaxial setting of central point of disc, bear and coil six bracing pieces of equidistant fixedly connected with along circumference on the outer wall, the bracing piece is kept away from bear the one end of dish and rotate and be connected with the back shaft, it places frame fixed connection and is in back shaft one end to observe the box, the one end of back shaft other end fixedly connected with connecting plate, the other end of connecting plate articulates there is the slider, the slider with annular spout looks adaptation, it is provided with the connecting rod on placing the frame to observe the box, the connecting rod both ends respectively with adjacent two it is articulated to observe the box and place the frame.
Preferably, the top end of the observation box is detachably connected with a box cover, the bottom end of the observation box is slidably connected with a bottom plate, and a plurality of vent holes are formed in the box cover, the bottom plate and the side wall of the observation box.
Preferably, the humidity control mechanism comprises a humidity control box fixedly connected to the inner wall of the treatment cavity, a water injection hole is formed in the outer side wall of the test box body, the humidity control box is communicated with the water injection hole, and a sealing plug is arranged on the water injection hole.
Preferably, the gas control mechanism comprises a gas inlet and a gas outlet which are arranged on the outer side wall of the test box body, the gas inlet and the gas outlet are communicated with the treatment cavity, the gas inlet is fixedly connected with an external gas cylinder through a pipeline, the gas outlet is fixedly connected with a gas concentration detector through a pipeline, and the gas inlet and the gas outlet are both provided with gas switch valves.
Preferably, the pressure control mechanism comprises a U-shaped pressure gauge fixedly connected to the test box body, one end of the U-shaped pressure gauge is communicated with the air outlet through a pipeline, and the other end of the U-shaped pressure gauge is open.
Preferably, the pressing mechanism comprises two fixed seats fixedly connected to the front end of the box cover, the front end of the test box body is fixedly connected with two hooks, and the hooks are matched with the fixed seats.
Preferably, two holes are formed in the front end of the test box body and communicated with the operation cavity, sealing gloves are fixedly connected to the holes, a threaded opening is formed in the outer side wall of the test box body and communicated with the operation cavity, and a threaded cover plate is connected to the threaded opening in a threaded mode.
A method for testing hypoxia stress of grain storage insects is suitable for a device for testing hypoxia stress of grain storage insects, and comprises the following steps:
a. placing the insects to be tested: injecting clear water or saturated salt solution into the humidity control mechanism, and placing insects with the same growth state and number into the insect observation mechanism;
b. adjusting the gas concentration: adjusting the concentration of the gas in the processing chamber through a gas control mechanism;
c. and (3) carrying out sealed observation: volatilizing the clear water or the saturated salt water in the step a to a target value, and sealing the test box body for observation;
d. adjusting the pressure: adjusting the internal pressure of the test box body through a pressure control mechanism;
e. taking out a sample for observation: insect samples were taken from the test chamber for observation.
The invention discloses the following technical effects:
the method is suitable for researching the reaction of grain storage pests to low-oxygen environments under the conditions of different humidity and gas concentration under the normal pressure condition, samples can be stored and checked at any time, a plurality of groups of insects can be observed and tested, the stress reaction of the insects is reduced in the observation process, the accuracy of test data is guaranteed, the grain storage insect low-oxygen stress test is facilitated, the test effect is guaranteed, and the test efficiency is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic diagram of the front structure of the apparatus of the present invention;
FIG. 2 is a schematic side view of the apparatus of the present invention;
FIG. 3 is a schematic view of the internal structure of the apparatus of the present invention;
FIG. 4 is an enlarged view of a portion of FIG. 3 according to the present invention;
FIG. 5 is a schematic view of the annular chute of the present invention;
FIG. 6 is a schematic view of a carrier structure according to the present invention;
FIG. 7 is a schematic view of the observation box according to the present invention;
FIG. 8 is a schematic view of the connection state of the connecting rod according to the present invention;
wherein, 1, a test box body; 2. a transverse plate; 3. a treatment chamber; 4. an operating chamber; 5. a through hole; 6. a box cover; 7. a disc; 8. an annular chute; 9. a drive shaft; 10. a crank; 11. an observation box placing frame; 12. a carrier tray; 13. a support bar; 14. a support shaft; 15. a connecting plate; 16. a slider; 17. a box cover; 18. a base plate; 19. a humidity control box; 20. a water injection hole; 21. a sealing plug; 22. an air inlet; 23. an air outlet; 24. an outer gas cylinder; 25. a gas concentration detector; 26. a gas switching valve; 27. a U-shaped pressure gauge; 28. a fixed seat; 29. hooking; 30. a hole; 31. a threaded opening; 32. a threaded cover plate; 33. a connecting rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
The invention provides a grain storage insect hypoxia stress test device, which comprises a test box body 1, wherein a transverse plate 2 is fixedly connected in the test box body 1, the transverse plate 2 divides the test box body 1 into a treatment cavity 3 and an operation cavity 4, the treatment cavity 3 is positioned above the operation cavity 4, a plurality of through holes 5 are formed in the transverse plate 2, the treatment cavity 3 is communicated with the operation cavity 4 through the through holes 5, through hole covers are connected to the through holes 5 in a threaded manner and are used for dividing the treatment cavity 3 and the operation cavity 4 into two sealed cavities, a plurality of insect observation mechanisms are arranged in the treatment cavity 3 and respectively correspond to the through holes, a humidity control mechanism, a gas control mechanism and a pressure control mechanism are arranged on the test box body 1, the humidity control mechanism, the gas control mechanism and the pressure control mechanism are all correspondingly arranged in the test box body 1, and a box cover 6 is hinged to the top end of the test box body 1, a pressing mechanism is arranged on the box cover 6, and the box cover 6 is in limit fit with the test box body 1 through the pressing mechanism.
In order to further optimize the scheme, in order to observe insects conveniently, the insect observation mechanism comprises a disc 7 fixedly connected to the inner wall of the treatment cavity 3, the front end of the disc 7 is provided with an annular chute 8, the central point of the annular chute 8 is staggered with the central point of the disc 7, the middle part of the disc 7 is rotatably connected with a transmission shaft 9, one end of the transmission shaft 9 extends out of the test box body 1 and is fixedly connected with a crank 10, the other end of the transmission shaft 9 is fixedly connected with a bearing piece, the bearing piece is arranged corresponding to the annular chute 8, the bearing piece is provided with a plurality of observation box placing frames 11, the observation box placing frames 11 are detachably connected with observation boxes, the observation boxes are arranged corresponding to the through holes 5, simultaneously for the convenience of observation, experimental box 1, case lid 6, observation box etc. are transparent material, place the insect in observing the box, will observe the box afterwards and place on bearing the piece, are convenient for carry out experimental observation to the different group insects in later stage.
In order to realize the observation of insects in different observation boxes, the bearing part comprises a bearing disc 12 fixedly connected on a transmission shaft 9, the central point of the bearing disc 12 is coaxial with the central point of a disc 7, six support rods 13 are fixedly connected on the outer wall of the bearing disc 12 at equal intervals along the circumferential direction, one end of each support rod 13 far away from the bearing disc 12 is rotatably connected with a support shaft 14, an observation box placing frame 11 is fixedly connected at one end of the support shaft 14, the other end of the support shaft 14 is fixedly connected with one end of a connecting plate 15, the other end of the connecting plate 15 is hinged with a sliding block 16, the sliding block 16 is matched with an annular sliding chute 8, a connecting rod 33 is rotatably connected on the observation box placing frame 11, the rotating point of the connecting rod 33 is positioned at one side of the middle part of the observation box placing frame 11, the six observation box placing frames 11 are divided into three groups, two observation box placing frames 11 in each group, and a connecting rod 33 is arranged between the two observation box placing frames 11 in each group, when the slider 16 slides in the annular sliding chute 8, the connecting rod 33 between the two observation box placing frames 11 ensures that the observation box placing frames 11 are always in the horizontal direction, so that the observation box arranged above is always in the horizontal state when rotating, the stability of the observation box during observation is kept, and the stress reaction of insects is avoided.
Further optimization scheme, in order to facilitate the access insect, it can dismantle and be connected with lid 17 to observe the box top, puts into the insect through opening lid 17, observes box bottom sliding connection and has bottom plate 18, and the accessible takes off whole observation box when taking a sample also can take out inside insect through sliding bottom plate 18, and lid 17, bottom plate 18 and observation box all set up a plurality of air vents on placing frame 11 lateral wall and guarantee to observe the circulation of box and outside air.
Further optimize the scheme, in order to realize regulation and the record to inside humidity, humidity control mechanism includes humidity control box 19 of fixed connection on 3 inner walls of treatment chamber, has seted up water injection hole 20 on 1 lateral wall of experimental box, humidity control box 19 and water injection hole 20 intercommunication are provided with sealing plug 21 on the water injection hole 20, fixedly connected with humidity detector on 1 lateral wall of experimental box is used for the humidity data of record in the experimental box 1.
Further optimize the scheme, in order to realize the control to gaseous, gas control mechanism is including seting up air inlet 22 and the gas outlet 23 on experimental box 1 lateral wall, and air inlet 22 and gas outlet 23 all communicate with treatment chamber 3, and air inlet 22 passes through outside gas cylinder 24 of pipeline fixedly connected with, and gas outlet 23 passes through pipeline fixedly connected with gas concentration detector 25, all is provided with gas switch valve 26 on air inlet 22 and the gas outlet 23.
Further optimize the scheme, in order to realize the control to inside pressure, pressure control mechanism includes U type pressure gauge 27 of fixed connection on experimental box 1, and U type pressure gauge 27 one end passes through the pipeline and communicates with gas outlet 23, and U type pressure gauge 27 other end opening.
Further optimize the scheme, hold-down mechanism includes two fixing bases 28 of fixed connection at case lid 6 front end, and test box 1 front end fixedly connected with two couples 29, couple 29 and fixing base 28 looks adaptation.
Further optimize the scheme, for the convenience of experimental operation, two holes 30 have been seted up to 1 front end of experimental box, and hole 30 and operation chamber 4 intercommunication, equal fixedly connected with sealing glove on two holes 30, seted up threaded opening 31 on 1 lateral wall of experimental box, threaded opening 31 and operation chamber 4 intercommunication, threaded connection has threaded cover plate 32 on the threaded opening 31.
A method for testing hypoxia stress of grain storage insects is suitable for a device for testing hypoxia stress of grain storage insects, and comprises the following steps:
a. placing the insects to be tested: adding a certain amount of clear water or saturated salt water into the humidity control box 19, placing insects with the same growth state and number into the observation box, placing the observation box on the observation box placing frame 11, and closing the box cover 6;
b. adjusting the gas concentration: adjusting the gas concentration in the processing cavity 3 through an external gas bottle 24, and observing the value of a gas concentration detector 25 to a required value;
c. and (3) carrying out sealed observation: the inside humidity is adjusted through volatilization of clear water or saturated salt water in the humidity control box 19, the humidity value is observed through a humidity detector, the insects in the sealed test box body 1 are observed after the humidity value is adjusted to a required value, and meanwhile, the insects in different observation boxes can be observed through rotating the crank 10;
d. adjusting the pressure: the air outlet 23 is opened through the U-shaped pressure gauge 27 to adjust the internal pressure of the test box body 1;
e. taking out a sample for observation: when the sample needs to be taken out, the observation box is taken out from the observation box placing frame 11 through the sealing glove or the insect sample is directly taken out from the observation box, and the insect sample is taken out by opening the threaded cover plate 32.
The invention provides a low oxygen stress test device and method for grain storage insects, when in use, a certain amount of clear water or saturated salt water is added into a humidity control box 19, insects to be tested with the same growth state and number are placed into an observation box, the observation box is placed on an observation box placing frame 11, then a box cover 6 is covered, an external gas bottle 24 is connected, a gas inlet 22 and a gas outlet 23 are opened, the numerical value of a gas concentration detector 25 is observed, when the concentration obtains a target concentration, the gas inlet 22 and the gas outlet 23 are closed, the humidity in a test box body 1 is adjusted, the humidity in the test box body 1 is controlled by controlling the water surface area of the humidity control box 19, when the humidity gas concentration reaches a target value, a U-shaped 27 is connected, the gas outlet 23 is opened, the pressure in the test box body 1 is controlled to be equal to the atmospheric pressure, a pressure gauge is sealed for observation, when a sample needs to be taken out for observation, a cover in the middle of a transverse plate 2 is opened, the sample is taken from the treatment chamber 3 to the operation chamber 4 by means of a sealed glove and is taken out through the screw cover 32.
According to the low-oxygen stress test device and method for the grain storage insects, provided by the invention, a certain number of grain storage insects are raised, the reaction of the grain storage insects to a low-oxygen environment under the conditions of different humidity and gas concentration under the normal pressure condition can be researched, the samples can be stored and inspected at any time, the stress reaction of the insects is reduced in the observation process, the accuracy of test data is ensured, the low-oxygen stress test of the grain storage insects is facilitated, the test effect is ensured, and the test efficiency is improved.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (10)
1. The low-oxygen stress test device for the grain storage insects is characterized by comprising a test box body (1), wherein a transverse plate (2) is fixedly connected in the test box body (1), the transverse plate (2) divides the test box body (1) into a treatment cavity (3) and an operation cavity (4), the treatment cavity (3) is positioned above the operation cavity (4), a plurality of through holes (5) are formed in the transverse plate (2), the treatment cavity (3) and the operation cavity (4) are communicated through the through holes (5), a plurality of insect observation mechanisms are arranged in the treatment cavity (3), the insect observation mechanisms are respectively arranged corresponding to the through holes, a humidity control mechanism, a gas control mechanism and a pressure control mechanism are arranged on the test box body (1), the humidity control mechanism and the pressure control mechanism are all arranged corresponding to the inside of the test box body (1), the top end of the test box body (1) is hinged with a box cover (6), a pressing mechanism is arranged on the box cover (6), and the box cover (6) is in limit fit with the test box body (1) through the pressing mechanism.
2. The stored grain insect hypoxia stress test device according to claim 1, wherein: insect observation mechanism includes fixed connection disc (7) on process chamber (3) inner wall, annular spout (8) have been seted up to disc (7) front end, the central point of annular spout (8) with the crisscross setting of central point of disc (7), the middle part of disc (7) is rotated and is connected with transmission shaft (9), transmission shaft (9) one end is stretched out outer fixedly connected with crank (10) of experimental box (1), transmission shaft (9) other end fixedly connected with holds carrier, hold carrier with annular spout (8) correspond the setting, it places frame (11) to be provided with a plurality of observation boxes on holding carrier, it places to dismantle on frame (11) and is connected with the observation box to observe the box, observe the box with through-hole (5) correspond the setting.
3. The stored grain insect hypoxia stress test device according to claim 2, wherein: the bearing part comprises a bearing disc (12) fixedly connected to the transmission shaft (9), the central point of the bearing disc (12) and the central point of the disc (7) are coaxially arranged, six support rods (13) are fixedly connected to the outer wall of the bearing plate (12) at equal intervals along the circumferential direction, one end of the supporting rod (13) far away from the bearing disc (12) is rotatably connected with a supporting shaft (14), the observation box placing frame (11) is fixedly connected with one end of the supporting shaft (14), the other end of the supporting shaft (14) is fixedly connected with one end of a connecting plate (15), the other end of the connecting plate (15) is hinged with a sliding block (16), the sliding block (16) is matched with the annular sliding groove (8), the observation box placing frame (11) is provided with a connecting rod (33), and two ends of the connecting rod (33) are respectively hinged to the adjacent observation box placing frame (11).
4. The stored grain insect hypoxia stress test device according to claim 3, wherein: observe the box top and can dismantle and be connected with lid (17), observe box bottom sliding connection has bottom plate (18), lid (17) bottom plate (18) with all seted up a plurality of air vents on the observation box lateral wall.
5. The stored grain insect hypoxia stress test device according to claim 1, wherein: humidity control mechanism includes fixed connection and is in humidity control box (19) on treatment chamber (3) inner wall, water injection hole (20) have been seted up on experimental box (1) lateral wall, humidity control box (19) with water injection hole (20) intercommunication, be provided with sealing plug (21) on water injection hole (20).
6. The stored grain insect hypoxia stress test device according to claim 1, wherein: gas control mechanism is including seting up air inlet (22) and gas outlet (23) on experimental box (1) lateral wall, air inlet (22) with gas outlet (23) all with treatment chamber (3) intercommunication, air inlet (22) are through pipeline fixedly connected with outside gas cylinder (24), gas outlet (23) are through pipeline fixedly connected with gas concentration detector (25), air inlet (22) with all be provided with gas switch valve (26) on gas outlet (23).
7. The stored grain insect hypoxia stress test device according to claim 6, wherein: the pressure control mechanism comprises a U-shaped pressure gauge (27) fixedly connected to the test box body (1), one end of the U-shaped pressure gauge (27) is communicated with the air outlet (23) through a pipeline, and the other end of the U-shaped pressure gauge (27) is opened.
8. The stored grain insect hypoxia stress test device according to claim 3, wherein: the pressing mechanism comprises two fixing seats (28) fixedly connected to the front end of the box cover (6), two hooks (29) are fixedly connected to the front end of the test box body (1), and the hooks (29) are matched with the fixing seats (28).
9. The stored grain insect hypoxia stress test device according to claim 4, wherein: two holes (30) have been seted up to test box (1) front end, hole (30) with operation chamber (4) intercommunication, two equal fixedly connected with sealing gloves on hole (30), threaded opening (31) have been seted up on test box (1) lateral wall, threaded opening (31) with operation chamber (4) intercommunication, threaded connection has threaded cover board (32) on threaded opening (31).
10. A method for testing hypoxia stress of grain storage insects, which is applicable to the device for testing hypoxia stress of grain storage insects in claim 1, and is characterized in that: the method comprises the following steps:
a. placing the insects to be tested: injecting clear water or saturated salt solution into the humidity control mechanism, and placing insects with the same growth state and number into the insect observation mechanism;
b. adjusting the gas concentration: the gas concentration in the processing cavity (3) is adjusted through a gas control mechanism;
c. and (3) carrying out sealed observation: volatilizing the clear water or the saturated salt water in the step a to a target value, and sealing the test box body (1) for observation;
d. adjusting the pressure: the internal pressure of the test box body (1) is adjusted through a pressure control mechanism;
e. taking out a sample for observation: the insect sample is taken out from the test box body (1) for observation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210486115.0A CN114794025B (en) | 2022-05-06 | 2022-05-06 | Device and method for testing hypoxia stress of stored-grain insects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210486115.0A CN114794025B (en) | 2022-05-06 | 2022-05-06 | Device and method for testing hypoxia stress of stored-grain insects |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114794025A true CN114794025A (en) | 2022-07-29 |
| CN114794025B CN114794025B (en) | 2023-08-29 |
Family
ID=82511881
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210486115.0A Active CN114794025B (en) | 2022-05-06 | 2022-05-06 | Device and method for testing hypoxia stress of stored-grain insects |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114794025B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204594963U (en) * | 2015-05-08 | 2015-08-26 | 河南工业大学 | Circulating carbon dioxide detector |
| CN205093389U (en) * | 2015-10-10 | 2016-03-23 | 华南农业大学 | Observe simple and easy device that stores up grain pest horizontal proliferation activity |
| CN106258633A (en) * | 2016-10-21 | 2017-01-04 | 山西大学 | A kind of animals and plants incubator of real-time Simulation wild environment |
| CN109490364A (en) * | 2018-12-29 | 2019-03-19 | 河南工业大学 | A kind of peanut pod water transport variation test device |
| CN209931261U (en) * | 2019-05-29 | 2020-01-14 | 河南工业大学 | Device for observing motion behaviors of stored grain insects |
| CN211832469U (en) * | 2020-03-19 | 2020-11-03 | 山东省蚕业研究所 | Black soldier fly egg incubator |
| CN216314924U (en) * | 2021-10-25 | 2022-04-19 | 王健力 | Biological information observation incubator for insects |
-
2022
- 2022-05-06 CN CN202210486115.0A patent/CN114794025B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204594963U (en) * | 2015-05-08 | 2015-08-26 | 河南工业大学 | Circulating carbon dioxide detector |
| CN205093389U (en) * | 2015-10-10 | 2016-03-23 | 华南农业大学 | Observe simple and easy device that stores up grain pest horizontal proliferation activity |
| CN106258633A (en) * | 2016-10-21 | 2017-01-04 | 山西大学 | A kind of animals and plants incubator of real-time Simulation wild environment |
| CN109490364A (en) * | 2018-12-29 | 2019-03-19 | 河南工业大学 | A kind of peanut pod water transport variation test device |
| CN209931261U (en) * | 2019-05-29 | 2020-01-14 | 河南工业大学 | Device for observing motion behaviors of stored grain insects |
| CN211832469U (en) * | 2020-03-19 | 2020-11-03 | 山东省蚕业研究所 | Black soldier fly egg incubator |
| CN216314924U (en) * | 2021-10-25 | 2022-04-19 | 王健力 | Biological information observation incubator for insects |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114794025B (en) | 2023-08-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US3992265A (en) | Antibiotic susceptibility testing | |
| CA2746152C (en) | Dynamic control system and method for controlled atmosphere room | |
| US7191670B2 (en) | Automatic microbial air sampling system and method | |
| US5525512A (en) | Incubator | |
| CN107804814A (en) | The axle of cryopreservation tube three is uncapped machine | |
| US20150309038A1 (en) | Method and device for marking isotopes | |
| CN105505762A (en) | Dynamic monitoring type bacterial culture equipment | |
| CN106190840B (en) | Embryo Culture device and its bracket | |
| CN209950078U (en) | Airtight storehouse for animal experiments | |
| CN114794025A (en) | Low-oxygen stress test device and method for grain storage insects | |
| CN117343841B (en) | Sealing device and rotary disc type incubator with sealing device | |
| CN208844100U (en) | A kind of cell incubator that can monitor cell growth state | |
| CA2981275C (en) | Whole plant gas exchange chamber using pressurization and ventilation of separated canopy and root zones | |
| US20140377843A1 (en) | Automated microbiology laboratory instrument and system uses thereof | |
| CN116047026A (en) | Soil microorganism breathes with continuous measuring device | |
| CN216614678U (en) | Medical science microbial cultivation case that facilitates use | |
| JP2009118862A (en) | Incubator | |
| CN220282064U (en) | Inspection sample storing compartment | |
| CN217133128U (en) | Medicine test device | |
| CN109804751A (en) | Accelerate the method, apparatus and gained Aged Seeds of Seed Aging | |
| CN108467834B (en) | Pressure-controllable small cell culture device | |
| CN211861021U (en) | Rice seed germinating bed | |
| CN211255894U (en) | Movable illumination box convenient for observing superoxide dismutase activity reaction | |
| CN213036329U (en) | Food detection sample save set | |
| CN221139206U (en) | Food detection sample storage device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |