CN114788806A

CN114788806A - Traditional Chinese medicine composition fermented primary pulp with antioxidant and whitening integrated effects and preparation method and application thereof

Info

Publication number: CN114788806A
Application number: CN202210537341.7A
Authority: CN
Inventors: 刘青; 周娟; 闫利娟
Original assignee: Calais Jinan Life Technology Co ltd
Current assignee: Calais Jinan Life Technology Co ltd
Priority date: 2022-05-18
Filing date: 2022-05-18
Publication date: 2022-07-26

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a traditional Chinese medicine composition fermented raw pulp with antioxidant and whitening integrated effects, and a preparation method and application thereof. The fermentation raw stock is prepared by the following method: mixing a traditional Chinese medicine composition, a bacterial liquid and water to obtain an initial system, performing fermentation culture on the initial system to obtain a fermentation broth, and sterilizing and centrifuging the fermentation broth to obtain a supernatant, namely fermentation raw stock, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 20-50 parts of angelica, 20-50 parts of liquorice and 20-50 parts of aloe. According to the invention, the traditional Chinese medicine composition is fermented by the bacterial liquid, so that the efficacy and activity of the traditional Chinese medicine components are retained, the loss of the active components caused by the traditional extraction mode is avoided, the preparation process is simple, the condition is mild, the industrial large-scale production can be realized, good economic benefits can be brought to enterprises, and the method has a wide prospect.

Description

Traditional Chinese medicine composition fermented raw juice with antioxidant and whitening integrated effects and preparation method and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a traditional Chinese medicine composition fermented raw pulp with antioxidant and whitening integrated effects, and a preparation method and application thereof.

Background

In recent years, with the rapid development of the cosmetic industry, the green concept is widely applied. The plant extract is natural and safe, and has small toxic and side effects, so that the plant extract is widely applied to the anti-aging whitening cosmetics as an active raw material. Along with consumer demand, there is an increasing spectrum of natural herbal skin care in the cosmetics industry. The extraction of functional effective substances from natural plants is a hot spot in the research of cosmetic ingredients at present. The Chinese herbal medicine serving as a natural antioxidant, a whitening agent and a preservative has wide development and application prospects in the field of cosmetics.

The Chinese herbal medicine contains rich biological components such as vitamins, amino acids, minerals, polysaccharides, flavones and polyphenols, and has the effects of moisturizing, whitening, soothing allergy, delaying aging and the like. The effective components can be enriched by utilizing the microbial fermentation technology, the molecular weight is reduced, the effective components are easy to be absorbed by the skin, and meanwhile, the toxic and side effects are greatly reduced by reconstruction.

By adopting a microbial fermentation technology, the structure of active matters in plant raw materials can be modified and reformed by utilizing microbial cells or an enzyme catalytic reaction system in the cells, so that valuable new products are obtained. In addition, fermentation is also a process of converting carbohydrates and sugars into skin-preferred enzymes and amino acids for our skin through the metabolic activity of probiotics such as lactic acid bacteria. For example, the yeast fermentation product filtrate is not added with a drop of water, the components of the yeast fermentation product filtrate are close to skin cells, and sensitive muscles can also accept the yeast fermentation product filtrate. Thus, unlike the manufacturing process of conventional skin care products in which actives and other costs are mixed at high temperatures, bio-fermented skin care products are manufactured by a slow fermentation process that lasts for several weeks in a clean, oxygen-free environment. The obtained active substance has higher content and better regulation effect on the skin micro-ecology.

The angelica has the main functions of a skin conditioner and an antioxidant in cosmetics and skin care products. Based on the effects of the angelica on promoting blood circulation, removing blood stasis and improving skin microcirculation, the Chinese angelica can be used for a plurality of cosmetics for promoting blood circulation, such as hair growth, regulating skin secretion, supplementing nutrition, relieving black eye and the like; the proliferation of the angelica on vascular endothelial cells shows that the angelica has the function of solidifying capillary vessels while promoting blood circulation; angelica has good SOD-like activity, inhibits membrane lipid peroxidation and free radical reaction caused by superoxide radicals, protects the damage of membrane lipid by combining with biomembrane phospholipid and other mechanisms to antagonize free radicals to tissues, and has special effects on dark complexion or sore and swelling caused by free radical retardation; has anti-aging effect on the activation rate of fluorescent enzyme and the proliferation effect on stem cells.

The Glycyrrhrizae radix extract has antioxidant, skin whitening, speckle removing, and antiinflammatory effects in cosmetics. The Glycyrrhrizae radix contains licoflavone, glycyrrhizic acid, etc. Licoflavone in Glycyrrhrizae radix is whitening component, and has certain whitening and sunscreen effects. Licoflavone is a natural whitening agent extracted from specific variety of licorice, can inhibit the activity of tyrosinase, inhibit the activity of dopachrome interconversion and DHICA oxidase, and is a fast, efficient and green cosmetic additive for whitening and removing spots.

Aloe extract has been widely used in cosmetics industry, and contains various components for eliminating superoxide free radical, such as superoxide dismutase and catalase, and has effects of caring skin, improving skin elasticity, preventing corrosion and delaying aging. The aloe gel is a natural sunscreen component, and can effectively inhibit ultraviolet rays in sunlight, prevent pigmentation and keep the skin white. The study finds that the aloe has the effects of astringing, softening and softening the skin, moisturizing, diminishing inflammation, relieving sclerosis and keratinization, improving scars and the like. The polysaccharide is the main saccharide compound of aloe, is also the main physiological active substance of aloe, and is rich in anthraquinone compounds (aloin, aloe-emodin, isoaloin, emodin, etc.), various trace elements, amino acids, vitamins, phenols, etc.

The invention screens natural traditional Chinese medicine components with the skin repairing effect, provides a safe and effective cosmetic from the perspective of Chinese herbal medicine fermentation, oxidation resistance and whitening, and has development prospect and economic value.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides the traditional Chinese medicine composition fermentation raw stock with the integrated antioxidant and whitening effects and the preparation method and application thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

one of the purposes of the invention is to provide a preparation method of a traditional Chinese medicine composition fermented raw stock with antioxidant and whitening integrated effects, which comprises the following steps: mixing the traditional Chinese medicine composition, the bacterial liquid and water to obtain an initial system, performing fermentation culture on the initial system to obtain fermentation liquor, and sterilizing and centrifuging the fermentation liquor to obtain supernatant, namely the fermentation protoplasm.

The traditional Chinese medicine composition comprises the following components in parts by mass: 20-50 parts of angelica, 20-50 parts of liquorice and 20-50 parts of aloe.

According to the invention, the traditional Chinese medicine composition is fermented by the bacterial liquid, so that the efficacy and activity of the traditional Chinese medicine components are retained, the loss of the active components caused by the traditional extraction mode is avoided, the preparation process is simple, the condition is mild, the industrial large-scale production can be realized, the good economic benefit can be brought to enterprises, and the method has a wide prospect.

The preparation method comprises the following specific steps:

(1) stock preparation

Removing impurities from radix Angelicae sinensis, Glycyrrhrizae radix and Aloe, cleaning, oven drying, and pulverizing to 100 mesh;

(2) strain culture

Activating strains: selecting a bacterial colony I, placing the bacterial colony I in a liquid culture medium, and activating the strain in a shaking table to obtain activated bacterial liquid;

and (3) strain purification: diluting and plating the activated bacteria liquid in a gradient manner so as to obtain a single bacterial colony and obtain a purified strain;

and (3) strain amplification culture: will be pureInoculating the strain into MRS culture medium (pH 7.0), culturing in shaking table at 25 deg.C, and obtaining zymocyte liquid when OD = 0.6-1.0, wherein the strain is in log phase and has concentration of (10) ⁶ ～10 ⁸ CFU/mL)；

And (3) strain amplification culture: inoculating the purified strain into MRS culture medium (pH value of 7.0), standing and culturing in 37 deg.C incubator, and obtaining zymocyte liquid when OD value = 0.6-1.0, wherein the strain is in logarithmic phase and has concentration of (10) ⁶ ～10 ⁸ CFU/mL)。

The preparation concentration is 10 ⁶ ～10 ⁸ Adjusting the pH value of the cfu/mL zymocyte liquid to 6.5-7.5;

(3) fermentation of

Mixing the bacterial liquid, the traditional Chinese medicine composition and water according to the proportion, and fermenting for 40 hours in a constant-temperature incubator at 37 ℃ to obtain fermentation liquid;

(4) purification of

Filtering the obtained fermentation liquor, sterilizing the filtrate at 110 ℃ for 30min under high pressure to inactivate strains, centrifuging the sterilized fermentation filtrate for 30min under the conditions of 12000r/min and the centrifugal radius of 9cm, removing the precipitate, and collecting the supernatant, namely the traditional Chinese medicine composition fermentation raw stock;

preferably, the usage ratio of the zymophyte liquid, the traditional Chinese medicine composition and water in the traditional Chinese medicine composition fermentation protoplasm is 0-30 mL: 10 g-80: 50-600 g.

Preferably, in the zymocyte liquid, the strain is saccharomycetes, more specifically saccharomyces cerevisiae (c)Saccharomyces cerevisiae). The strain preservation information is as follows: guangdong province microbial culture Collection (GDMCC), accession number: the preservation number of building No. 59 of Ji No. 100 of the prefecture Zhonglu city is one of the following: GDMCC2.71, GDMCC2.132, GDMCC2.74, GDMCC strain corresponding to the date of deposit: 2016, 1 month, 1 day, with classification name: saccharomyces cerevisiaeSaccharomyces cerevisiae Hansen。

Preferably, in the zymocyte liquid, the strain is lactobacillus, more specifically lactobacillus delbrueckii subsp bulgaricus (subsp. bulgaricus)Lactobacillus delbrueckii subsp.Bulgaricus). The strain has preservation information ofName of Tibetan unit: china center for Industrial microbial culture Collection (CICC), the storage unit address: no. 6 building of No. 24 Hospital of the Korean Xianqiao in Beijing city, the preservation number is one of the following: CICC6103, CICC6097 and CICC6101, wherein the corresponding preservation date of strains is as follows: 31/2006, 1/2007, with the corresponding taxonomic designations: lactobacillus delbrueckii subspecies bulgaricusLactobacillus delbrueckii subsp. bulgaricus。

The strains are all the strains existing in the prior art.

Preferably, the Chinese medicinal raw materials of the angelica, the liquorice and the aloe are dry powder, and the particle size of the dry powder is 80-200 meshes, particularly 100 meshes.

The second purpose of the invention is to provide the Chinese herbal medicine fermented raw stock with the functions of resisting oxidation and whitening prepared by the method.

The third purpose of the invention is to provide an application of the fermented protoplasm of the traditional Chinese medicine composition in preparing cosmetics.

The cosmetic contains the fermented protoplasm of the traditional Chinese medicine composition, and the dosage of the fermented protoplasm is 5%.

Preferably, the cosmetic comprises a lotion, an emulsion, a cream, a mask, a cleanser or a BB cream.

Advantageous effects

The invention discloses a traditional Chinese medicine composition fermented raw stock with antioxidant and whitening integrated effects, and compared with the prior art, the traditional Chinese medicine composition fermented raw stock has the following advantages:

(1) the traditional Chinese medicines are reasonably compounded according to the principle of 'monarch, minister, assistant and guide' prescription, can realize synergistic interaction, maximally exert the whitening and beautifying effects of the raw materials, and have more comprehensive effects than single traditional Chinese medicine.

(2) The preparation process provided by the invention has high extraction rate, and the fermentation extraction method has the advantages that the production process can be effectively simplified, so that the reaction process is carried out under mild conditions. Meanwhile, the fermentation extraction method can also change the molecular structure of the components of the raw materials, and after microbial fermentation, active macromolecular substances in the Chinese herbal medicines are converted into small molecular substances which can be directly absorbed by intestinal tracts of animals, so that the raw material effect is improved, the toxicity and the irritation are reduced, the color, the taste and other advantages are optimized, and the method is an ideal novel process for extracting the traditional Chinese medicine and is favorable for popularization and application.

(3) The fermented raw juice of the traditional Chinese medicine composition can effectively brighten skin color when being used in cosmetics, has obvious and effective antioxidant effect, has good whitening effect and has huge market value.

Detailed Description

Hereinafter, the present invention will be described in detail. Before the description is made, it should be understood that the terms used in the present specification and the appended claims should not be construed as limited to general and dictionary meanings, but interpreted based on the meanings and concepts corresponding to technical aspects of the present invention on the basis of the principle that the inventor is allowed to define terms appropriately for the best explanation. Accordingly, the description proposed herein is just a preferable example for the purpose of illustrations only, not intended to limit the scope of the invention, so it should be understood that other equivalents and modifications could be made thereto without departing from the spirit and scope of the invention.

The following examples are given by way of illustration of embodiments of the invention and are not to be construed as limiting the invention, as those skilled in the art will appreciate that various modifications may be made without departing from the spirit and scope of the invention. Unless otherwise specified, reagents and equipment used in the following examples are commercially available products.

Example 1

A lactobacillus delbrueckii Chinese herbal medicine fermented raw pulp is prepared by the following method:

(1) stock preparation

Removing impurities from 20 parts of angelica, 20 parts of liquorice and 50 parts of aloe, cleaning, drying and crushing to 100 meshes;

(2) culture of bacterial species

Bacterial activation, namely picking bacterial colonies to be encircled in a liquid culture medium, and putting the bacterial colonies into a shaking table to activate the bacterial colonies to obtain activated bacterial liquid;

and (3) strain purification, namely diluting and paving the activated bacterium liquid in a gradient manner so as to obtain a single bacterial colony and obtain the purified strain.

And (3) strain amplification culture: and inoculating the purified strain into an MRS culture medium (with the pH value of 7.0), culturing in a shaker at 25 ℃, and obtaining zymophyte liquid when the OD value is = 0.6-1.0.

The preparation concentration is 10 ⁶ Adjusting the pH value of the cfu/mL zymocyte liquid to 6.5;

(3) fermentation of

Mixing the bacteria solution, radix Angelicae sinensis, Glycyrrhrizae radix, Aloe composition and water at a certain ratio, and fermenting in a constant temperature incubator at 37 deg.C for 40 hr to obtain fermentation liquid; the dosage ratio of the zymophyte liquid to the traditional Chinese medicine composition to the water is 20 mL: 80 g: 600 g.

(4) Purification of

Filtering the obtained fermentation liquor, carrying out autoclaving on the filtrate at the temperature of 110 ℃ for 30min to inactivate strains, centrifuging the sterilized fermentation filtrate for 30min under the conditions of 12000r/min and a centrifugation radius of 9cm, removing the precipitate, and collecting supernatant, namely the lactobacillus delbrueckii Chinese herbal medicine fermentation raw stock.

The strain is Lactobacillus delbrueckii subspecies bulgaricus (B.delbrueckii)Lactobacillus delbrueckii subsp.Bulgaricus). The strain preservation information is that the name of a preservation unit is as follows: china center for Industrial microbial culture Collection (CICC), the storage unit address: no. 6 building of No. 24 Hospital of the Chaoyang district wine, Beijing, with a preservation number of one of the following: CICC6103, CICC6097 and CICC6101, wherein the corresponding preservation date of strains is as follows: 31/2006, 1/2007, with the corresponding taxonomic designations: lactobacillus delbrueckii subspecies bulgaricusLactobacillus delbrueckii subsp. bulgaricus。

The strain is the strain existing in the prior art.

The lactobacillus delbrueckii is used for fermenting the traditional Chinese medicine composition, so that the efficacy and activity of the traditional Chinese medicine composition are kept, the loss of active ingredients caused by the traditional extraction mode is avoided, the preparation process is simple, the condition is mild, the industrial large-scale production can be realized, good economic benefits can be brought to enterprises, and the method has wide prospects.

Example 2

A saccharomyces cerevisiae Chinese herbal medicine fermentation raw pulp is prepared by the following method:

(1) stock preparation

50 parts of angelica, 50 parts of liquorice and 20 parts of aloe are subjected to impurity removal, cleaning, drying and crushing to 200 meshes;

(2) strain culture

Activating the strain, namely picking a bacterial colony to be encircled in a liquid culture medium, and putting the bacterial colony into a shaking table to activate the strain to obtain activated bacterial liquid;

and (4) strain purification, namely, diluting and paving the activated bacteria liquid in a gradient manner so as to obtain a single bacterial colony and obtain a purified strain.

And (3) strain amplification culture, namely inoculating the purified strain into a YM culture medium (with the pH value of 7.0), and performing static culture in an incubator at 37 ℃, wherein when the OD value = 0.6-1.0, a zymocyte liquid is obtained, and the strain is in a logarithmic phase.

The preparation concentration is 10 ⁸ Adjusting the pH value of the cfu/mL zymocyte liquid to 7.5;

(3) fermentation of

Mixing the bacteria solution, radix Angelicae sinensis, Glycyrrhrizae radix, Aloe composition and water at a certain ratio, and fermenting in a constant temperature incubator at 37 deg.C for 40 hr to obtain fermentation liquid; the dosage ratio of the zymophyte liquid, the traditional Chinese medicine composition and water is 30 mL: 10 g: 50 g.

(4) Purification of

The fermentation strain is saccharomyces cerevisiae (Saccharomyces cerevisiae). The strain preservation information is as follows: guangdong province microbial culture Collection (GDMCC), accession number: the preservation number of the Guangzhou city Pieli Zhongluo No. 100 large yard No. 59 is one of the following: GDMCC2.71, GDMCC2.132, GDMCC2.74, GDMCC strain corresponding to the date of deposit: 2016, 1 month, 1 day, with classification name: saccharomyces cerevisiaeSaccharomyces cerevisiae Hansen。

The strain is the strain existing in the prior art.

EXAMPLE 3 fermentation Effect test of different strains

In order to prove the effect of the Chinese herbal medicine fermented raw stock, the following effect experiment is carried out.

1. Free radical (DPPH) scavenging experiments

(1) The experimental method comprises the following steps:

the positive control was dissolved in 95% ethanol and diluted to: the test system was verified by using a series of concentration gradients of 0.08mg/mL, 0.04mg/mL, 0.02mg/mL, and 0.01 mg/mL.

Treating a test object: the water-soluble test substance is diluted with water to obtain a multi-concentration sample, and the oil-soluble test substance is diluted with 95% ethanol to obtain a multi-concentration sample.

Referring to Table 3, a 10mL test tube was used to set up the sample tube (T), sample background (T) ₀ ) DPPH tube (C) and solvent background (C) ₀ ) For each sample concentration to be tested, 3 parallel tubes are required for each sample tube (T), and 3 parallel tubes are required for DPPH tube (C).

In the sample tube (T) and sample background (T) ₀ ) 1mL of the same concentration of each sample solution was added.

In all test tubes (T, T) ₀ C, C), adding solvent, adding water to the water-soluble sample, adding 95% ethanol to the oil-soluble sample, adding 3mL of ethanol, and mixing.

1mL of DPPH ethanol solution was added to the sample tubes (T) and DPPH tube (C), with sample background (T) ₀ ) And solvent background (C) ₀ ) Replace with 95% ethanol, shake gently, and stand at room temperature for 5 minutes. Each reaction solution was transferred to a 1cm cuvette and absorbance was measured at 517 nm.

TABLE 3 sample addition requirements

Calculating DPPH free radical clearance rate:

clearance (100%) = f

）×100%

2. Tyrosinase activity inhibition assay

(1) The experimental method comprises the following steps:

the positive control was diluted with disodium phosphate-citric acid buffer ph6.8 to: a series of concentration gradients of 1mg/mL, 0.2mg/mL, 0.04mg/mL, 0.008mg/mL were used to validate the assay system.

Treating a test object: the test substance is diluted into a multi-concentration sample by using a disodium hydrogen phosphate-citric acid buffer solution.

Referring to Table 4, a 10mL test tube was used to set up the sample tube (T), sample background (T) ₀ ) Enzyme reaction tube (C) and solvent background (C) ₀ ) For each sample, 3 parallel tubes are required to be set for each sample tube (T) of each concentration to be tested, and 3 parallel tubes are required to be set for the enzyme reaction tube (C).

In the sample tube (T) and sample background (T) ₀ ) 1mL of the same concentration of each of the sample solutions, an enzyme reaction tube (C) and a solvent background (C) ₀ ) 1mL of disodium hydrogen phosphate-citric acid buffer was added.

Adding 0.5mL of tyrosinase solution into each of the sample tube (T) and the enzyme reaction tube (C), and obtaining a sample background (T) ₀ ) With background of solvent (C) ₀ ) Replacing with 0.5mL disodium hydrogen phosphate-citric acid buffer solution, mixing the sample and tyrosinase thoroughly, and incubating in a 37 ℃ water bath for 10 minutes.

And sequentially adding 2mL of levodopa solution into each tube, controlling the reaction time of each tube to be 5 minutes, immediately transferring each tube of reaction solution into a cuvette, and measuring the light absorption value at 475 nm.

TABLE 4 sample addition requirements

Calculation of tyrosinase inhibition:

clearance (100%) = (c) =

）×100%

3. Results of the experiment

TABLE 1 influence of different strains and order of addition on fermentation broth

The Chinese herbal medicine antioxidant fermented protoplasm of the liquorice, the angelica and the aloe is characterized by having certain whitening and antioxidant effects and better antioxidant effect. As can be seen from Table 1, the whitening and antioxidant effects of the Chinese herbal medicine mixture fermented by the Lactobacillus delbrueckii are better than those of 3 groups of fermentation of Saccharomyces cerevisiae alone, simultaneous addition and mixed fermentation of Saccharomyces cerevisiae and Lactobacillus delbrueckii, fermentation of Saccharomyces cerevisiae first, fermentation of Lactobacillus delbrueckii after 20h, and the like. Therefore, lactobacillus delbrueckii is selected to ferment the Chinese herbal medicine antioxidant whitening integrated fermented raw stock of liquorice, angelica and aloe.

TABLE 2 comparison of the Sterilization and non-Sterilization effects before fermentation of Lactobacillus delbrueckii Chinese herbal medicine fermentation raw stock

From table 2, the lactobacillus delbrueckii fermented raw stock solution sterilized before fermentation has better whitening effect and antioxidant effect, especially antioxidant effect, the Chinese herbal medicines of the angelica, the liquorice and the aloe after the lactobacillus delbrueckii fermented sterilization obviously improve the whitening effect of the angelica, the liquorice and the aloe, and compared with a positive control, the Chinese herbal medicine of the angelica, the liquorice and the aloe has the effect of 0.04mg/mLV mg/ _E The whitening effect is equivalent. Therefore, the lactobacillus delbrueckii angelica, liquorice and aloe fermented raw juice greatly improve the whitening effect of the angelica, the liquorice and the aloe. Meanwhile, the tyrosinase inhibition rate is equivalent to the effect of Chinese herbal medicine raw stock, is higher than that of a group which is not sterilized before fermentation, and is equivalent to the effect of the tyrosinase inhibition rate of 0.04mg/ml kojic acid. Therefore, the lactobacillus delbrueckii angelica sinensis, the liquorice and the aloe fermented primary pulp integrate whitening and oxidation resistance.

Example 4

Antioxidant double-layer base essence added with angelica sinensis, liquorice and aloe fermented primary pulp

Comprises the following components:

group A

10 portions of white Potentilla seed oil

8.075 parts of caprylic/capric triglyceride

Isononyl isononanoate 7.5 parts

1 portion of glycerin

0.6 part of jojoba seed oil

Group B

56.805 parts of deionized water

EDTA disodium 0.05 part

0.03 part of xanthan gum

2 portions of propylene glycol

1 part of glycerol

Group C

1 part of sodium hydroxide

1 part of deionized water

Group D

0.15 portion of sodium citrate

0.19 part of citric acid

1 part of deionized water

Group E

Glycolic acid 3.4 parts

0.2 part of panthenol

Spectrastat PHL 1 part

Chinese herbal medicine fermented raw stock 5 parts

The preparation method of the antioxidant double-layer substrate essence comprises the following steps:

(1) mixing group A uniformly in advance for later use;

(2) scattering the group B powder into water, uniformly stirring, heating to 80-82 ℃, completely dissolving uniformly, homogenizing for 1-2 minutes, and defoaming for later use;

(3) dissolving group C and D in advance for later use;

(4) cooling group B to below 40 deg.C, sequentially adding groups C, D and E, and stirring;

(5) group A was added slowly to (4).

Example 5

Antioxidant cream containing radix Angelicae sinensis, Glycyrrhrizae radix, and Aloe fermented stock

Comprises the following components:

group A

47.9 parts of deionized water

Allantoin 0.1 part

10 portions of glycerin

1 part of sodium chloride

1 part of stimulus inhibitory factor

Group B

0.6 part of PEG-3 polydimethylsiloxane

PEG-10 polydimethylsiloxane 0.4 part

21.5 parts of cyclopentadimethylsiloxane and cyclohexasiloxane

2.5 parts of polydimethylsiloxane

6 parts of squalane

Group C

Chinese herbal medicine fermented raw stock 8 parts

Group D

Spectrastat PHL 1 part

The preparation method of the antioxidant cream comprises the following steps:

(1) mixing and heating the group A to 80-85 ℃, stirring until the solid is completely melted into liquid, and keeping the temperature for later use;

(2) mixing and heating the group B to 80-85 ℃, stirring until the solid is completely melted into liquid, and keeping the temperature for later use;

(3) slowly adding the group A into the group B under weak and medium speed stirring, and homogenizing at medium speed for 1-3 minutes;

(4) add group C, D to (3) and stir at low speed for 5 minutes.

The above examples are only intended to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.

Claims

1. A preparation method of a traditional Chinese medicine composition fermented raw stock with antioxidant and whitening integrated effects is characterized by comprising the following steps: mixing a traditional Chinese medicine composition, a bacterial liquid and water to obtain an initial system, performing fermentation culture on the initial system to obtain a fermentation broth, and sterilizing and centrifuging the fermentation broth to obtain a supernatant, namely fermentation protoplasm, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 20-50 parts of angelica, 20-50 parts of liquorice and 20-50 parts of aloe.

2. The preparation method according to claim 1, which is specifically as follows:

(1) preparing materials: removing impurities from the raw materials of the Chinese medicinal composition, cleaning, oven drying, and pulverizing;

(2) and (3) strain culture: activating, purifying and enlarging culturing the strain, and then preparing the concentration to be 10 ⁶ ～10 ⁸ Adjusting the pH value of cfu/mL zymocyte liquid to 6.5-7.5;

(3) fermentation: mixing the bacterial liquid, the traditional Chinese medicine composition and water according to the proportion, and fermenting for 40 hours in a constant-temperature incubator at 37 ℃ to obtain fermentation liquid;

(4) and (3) purification: filtering the obtained fermentation liquor, sterilizing the filtrate under high pressure to inactivate the strains, centrifuging the sterilized fermentation filtrate, removing the precipitate, and collecting the supernatant to obtain the Chinese medicinal composition fermentation raw stock.

3. The preparation method according to claim 2, wherein in the step (1), the raw materials of the traditional Chinese medicine composition are pulverized to 80-200 meshes.

4. The method according to claim 2, wherein the step (2) of culturing the bacterial species specifically comprises:

activating strains: selecting a colony, namely, putting the colony in a liquid culture medium, and activating a strain in a shaking table to obtain activated bacterial liquid;

and (3) strain amplification culture: inoculating the purified strain into an MRS culture medium (the pH value is 7.0), culturing in a shaker at 25 ℃, and obtaining zymophyte liquid when the OD value is = 0.6-1.0; or inoculating the purified strain into an MRS culture medium (with the pH value of 7.0), and standing and culturing in an incubator at 37 ℃ to obtain a zymocyte liquid when the OD value is = 0.6-1.0.

5. The method according to claim 4, wherein in the step (2), the bacterial species in the fermented bacterial liquid is yeast or lactobacillus.

6. The traditional Chinese medicine composition fermented raw pulp with the integral antioxidant and whitening effects according to claim 2, characterized in that in the step (3), the usage ratio of the fermented bacterial liquid, the traditional Chinese medicine composition and water is 0-30 mL: 10-80 g: 50-600 g.

7. A Chinese medicinal composition fermented raw stock with antioxidant and whitening effects prepared by the method of any one of claims 1-6.

8. Use of the fermented raw juice of the traditional Chinese medicine composition with the antioxidant and whitening integrated effects prepared by the method of any one of claims 1 to 6 in preparation of cosmetics.

9. The use of claim 8, wherein the cosmetic comprises 5% of the fermented puree of the herbal composition.

10. Use according to claim 9, wherein the cosmetic product comprises a lotion, an emulsion, a cream, a mask, a cleanser or a BB cream.